Lag0001513 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0001513
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionPolyprenol reductase
Locationchr4: 32182975 .. 32186475 (+)
RNA-Seq ExpressionLag0001513
SyntenyLag0001513
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGCTCGGAATTGTTTGGCTTCTCAGAGCTGCTTGGATTGCAGCAATATTGCCTATACTTGTAGCTTTCTTACCGTTCTCTAGACTGAATTGGTTTCGTGAAATTGTGTTGGGGTTTGCGAAGAGAGGGAAGATCCTGCAATCGTCGTCTCAGGTAAGTGATCGAATTGTCTATTGTCGATGTACTTGACTATCTTTATTCCCCGTGGCTCTCTGATTAGAATGTCCGTTTGGTGGCTGAGAGAATTGAATTGCGGTGAGGAGAGTTGCAGAGCTTTTGAATTATGAGTTGCGTCATGGTTATTGGATGGAGGGTATCTATGTATTTTGCCTTTTTCTTTCAACAAATCACTTTTTATACGAAGAGGAGGATTGGAAATTTGAAATATGGTAGGGGACTCCAATCTGCTTAGTAATTAGACCATTAATCTTGGGTATTTCAGCTCTAATTTTCCTTAGAAGTCAATACATATTCTCACCTTAAAAAGTATTAATCATCTCAGTTAAACTTTTGCTAAAGTTAAATCACTAGATTCGTCCTTTGGTGAAAGTTTGAATTTTGTCCTTATAATTTGATAAAACCTCTATTATGAGAGTATTAGATGCCACTAATAGATTCTGTACATGGTGATTAGAAGCTACTAACAGAACTGCCACACGCTTAATCCGTTAAGGGGCTTCTAACTGTAATGACTATTTGTGAAAGTTTATCAAATCATAGGGTCAAAATCCTACCTTTGAAACTATGAGGATTAAGTTCTATTTTTTTTTTAAAACCATACCGACCAAATTTGTATTACCTTCATTCTAATAATCGATTGATCACTTAGCAATTGGTAATTAATGACATAAGGTTGTATGCCTATTTTTTTAGTTAGTCGTTAGTCCACCCATATTTTATCCTATTGACATCTTCTCTTGTACTACTCATTGCCACGTCATATGAAAATTATTTTTGCTAGTCGCTTGCATCGACTTATTAGGATCAAAATTGAAAATTCTTAGATGTAGGTGAAAAATTCTGAACATGATAAGATGTTCGTGAATTTTTCTGAAGTGCAGGGATAAATTAACACAACCATCAAAGTTCAATTTATAATTTAACCTTTGCTGACATTGTATAATCTTATTTACTCATTCTCCAATTTCAGAAATTCACTGTACCTCAGAAATTCTTCTGCCACTTCTATGTGTTTGCTGTTATCTGGACAACATTGTTACTAGGAACAAGTTGGATATATGCATATGTATCGACCCCAGAAATCCCAGAGCCATATAATTTTCCTGCCATTACCAGCCAGTTGACTGGAGGTTCAAGTTTGTTTTCATGGCAGAAATCTCATTCATCTCGGAGGGAGCAAACATTCCTGGTCTGGAAAGCTGTTTTTCTGCTTTTATTAATGGAAGTTCAAGTTTTGAGACGTCTTTATGAAACAATATTCGTTTTCAACTATAGTCCCTCGGCTCGGATGCACATCTTTGGCTATTTAACTGGCTTTTTGTAAGTACCATTTTGAAGCTCTCTTTAGCAGGTTTTATTGCAATATATATCCACTGCTTCTACTATGGTTAGTTTTTGGTATTTTGGGCCCTGCTTTAAGATGCTGTGGAAAGAATTAATACTAGAATCGAGATAGAAGATCTTGGTGTTTCAATCACGAATGCAAGTTTATACACTTGTACTACAAAAATTGCATTCTACATCCAAAATAGCCATAAGAAGTCATAGCTTTTGGTTTGGCATCCTGTTCTTGATTGTCTTATCAACGGAAAAAATGTTTACCTTTCTGGCTGATTGTGGGCAAGAAGCCCTAAACGCCATGGTTTGTAGCTACATGCTCTAATCTTTGTGTGGGATATGTTAAATCAATTTGTTTACCGTTTGCTTCTCAGGATGGAAAATACTTTTTGGGTGAATTTTCTTTTTCCAATTAATTTGTATTTATTTTGTTTTTACATATGTAAGAATGTGGTATGCTGTATTCTGTTGCTGGGTTCTAAAAACTGTCATAGATAAATGCTTTGGTGATTCATTGTTATTTGTTATAGTTGAATCCTTACGTCTGGAGGTTTGGTTTGTTGATTTCTATTATTTTCAATGGTTAGTTTCTACACAGCAGCTCCTCTATCACTTTGTAGCAATTGTCTTCCAGAAGTTTATCACTTTGCCGCAGATGGTCTAGCTCAGTTCGTTTTTAAAAGCAAAATGCAAATGCCAGATGTTGAAGTTAGTCTGTTGGATGTTGTGATTCCTTTAACAAATTTAGGGTGGCGTCAGTGGATTGGGGCAGCTTTATTCTTTTGGGGTTGGATTCATCAGCAGCGTTGTCATCTAATTCTGGTACGATACTCCTCTTCCATCGTAAATTGTTTATTCAGATATTGGATTTATGATGGAATTTGAATTAGTTTTCAGTTCACATTGCCTTCTGATCCTTCATTATTAACAGATTCTTCAGAATTGAAAAATGTTTTGTATCTAGAACAGAACTTTTAATTCTCGAAGGCGTTAAAAAATTTTCTCATCAGACAGATTGAAAATAAAATGATTGAAAAACCGTTGGAGTTTGAAGATTTTAGTATAGTATGGAACCATTTTTTCATTAGAATCTAAAAATGTGCTTAATTGGTTAATTGTGTGTGAACTTTTTCTATGAATGCCATGCCACAATATGAGTCCCTTACACGAGGGCGCTGATACCAGTTTTGGTGTAAACCAAAGAATGAAAAAAATAAACTTCACAATCGAACTGCCCTTGAATAAAATAAGGCTGATTATTTATACAACCTATAGGCTGTTAACAACCCAGCCAAGAATCCAAGATATCTAACTAACTAAATTACAAAAGTAATAGTCTAGTAACTAAAGTAAAACAAAACAGTAAAATAGAAGAATAACCCAAGGCTTTTACCAAAAGCCTACATCACAATATATTTAAACACAAGTGCTGGATTCATTTGACTGATACACAGCAAGGTTAATTTGCTTAGGGCTCGCTGCGGGTACGTGGAGGACGAACCGAAGAATATAGAATTCCTCATGGTGATTGGTTCGAAATTGTGTCTTCTCCACACTATCTCGCTGAGATCGTAAGTTTCTCCCTCATGAACTTGCAATGGACCAACTGCAAATTTTCTTGTCACTAATTCTTAGCTATTACTATTTTCCTTTAAAAGGTTATATATGCTGGATTTGTGGTTGCTAGTGGAGGAGAAGACTTCACAATATGGCTACTTTTTGGATTTGTGGTGAGATTCTTATTTTTTCTGAGAGTTAAAATGCAGTTTTCTCTTTTCTTCCATTTCTTTATAATTACTTACTGTTTCAACCTCCATAGTCATGTGTGGGACTAAAGATGGAGAAGGGGAATAATCTTCTGTTGGGTGTTTGCAGGTGGCAAATCTGGCCTTTGCAGCAGCAGAAACTCATAGGTGGTATTTTCGTAAATTCGATAATTATCCGAGAAACCGGTTTGCAGTTATACCATATATCTTTTGA

mRNA sequence

ATGGAGCTCGGAATTGTTTGGCTTCTCAGAGCTGCTTGGATTGCAGCAATATTGCCTATACTTGTAGCTTTCTTACCGTTCTCTAGACTGAATTGGTTTCGTGAAATTGTGTTGGGGTTTGCGAAGAGAGGGAAGATCCTGCAATCGTCGTCTCAGAAATTCACTGTACCTCAGAAATTCTTCTGCCACTTCTATGTGTTTGCTGTTATCTGGACAACATTGTTACTAGGAACAAGTTGGATATATGCATATGTATCGACCCCAGAAATCCCAGAGCCATATAATTTTCCTGCCATTACCAGCCAGTTGACTGGAGGTTCAAGTTTGTTTTCATGGCAGAAATCTCATTCATCTCGGAGGGAGCAAACATTCCTGGTCTGGAAAGCTGTTTTTCTGCTTTTATTAATGGAAGTTCAAGTTTTGAGACGTCTTTATGAAACAATATTCGTTTTCAACTATAGTCCCTCGGCTCGGATGCACATCTTTGGCTATTTAACTGGCTTTTTTTTCTACACAGCAGCTCCTCTATCACTTTGTAGCAATTGTCTTCCAGAAGTTTATCACTTTGCCGCAGATGGTCTAGCTCAGTTCGTTTTTAAAAGCAAAATGCAAATGCCAGATGTTGAAGTTAGTCTGTTGGATGTTGTGATTCCTTTAACAAATTTAGGGTGGCGTCAGTGGATTGGGGCAGCTTTATTCTTTTGGGGTTGGATTCATCAGCAGCGTTGTCATCTAATTCTGGGCTCGCTGCGGGTACGTGGAGGACGAACCGAAGAATATAGAATTCCTCATGGTGATTGGTTCGAAATTGTGTCTTCTCCACACTATCTCGCTGAGATCGTTATATATGCTGGATTTGTGGTTGCTAGTGGAGGAGAAGACTTCACAATATGGCTACTTTTTGGATTTGTGGTGGCAAATCTGGCCTTTGCAGCAGCAGAAACTCATAGGTGGTATTTTCGTAAATTCGATAATTATCCGAGAAACCGGTTTGCAGTTATACCATATATCTTTTGA

Coding sequence (CDS)

ATGGAGCTCGGAATTGTTTGGCTTCTCAGAGCTGCTTGGATTGCAGCAATATTGCCTATACTTGTAGCTTTCTTACCGTTCTCTAGACTGAATTGGTTTCGTGAAATTGTGTTGGGGTTTGCGAAGAGAGGGAAGATCCTGCAATCGTCGTCTCAGAAATTCACTGTACCTCAGAAATTCTTCTGCCACTTCTATGTGTTTGCTGTTATCTGGACAACATTGTTACTAGGAACAAGTTGGATATATGCATATGTATCGACCCCAGAAATCCCAGAGCCATATAATTTTCCTGCCATTACCAGCCAGTTGACTGGAGGTTCAAGTTTGTTTTCATGGCAGAAATCTCATTCATCTCGGAGGGAGCAAACATTCCTGGTCTGGAAAGCTGTTTTTCTGCTTTTATTAATGGAAGTTCAAGTTTTGAGACGTCTTTATGAAACAATATTCGTTTTCAACTATAGTCCCTCGGCTCGGATGCACATCTTTGGCTATTTAACTGGCTTTTTTTTCTACACAGCAGCTCCTCTATCACTTTGTAGCAATTGTCTTCCAGAAGTTTATCACTTTGCCGCAGATGGTCTAGCTCAGTTCGTTTTTAAAAGCAAAATGCAAATGCCAGATGTTGAAGTTAGTCTGTTGGATGTTGTGATTCCTTTAACAAATTTAGGGTGGCGTCAGTGGATTGGGGCAGCTTTATTCTTTTGGGGTTGGATTCATCAGCAGCGTTGTCATCTAATTCTGGGCTCGCTGCGGGTACGTGGAGGACGAACCGAAGAATATAGAATTCCTCATGGTGATTGGTTCGAAATTGTGTCTTCTCCACACTATCTCGCTGAGATCGTTATATATGCTGGATTTGTGGTTGCTAGTGGAGGAGAAGACTTCACAATATGGCTACTTTTTGGATTTGTGGTGGCAAATCTGGCCTTTGCAGCAGCAGAAACTCATAGGTGGTATTTTCGTAAATTCGATAATTATCCGAGAAACCGGTTTGCAGTTATACCATATATCTTTTGA

Protein sequence

MELGIVWLLRAAWIAAILPILVAFLPFSRLNWFREIVLGFAKRGKILQSSSQKFTVPQKFFCHFYVFAVIWTTLLLGTSWIYAYVSTPEIPEPYNFPAITSQLTGGSSLFSWQKSHSSRREQTFLVWKAVFLLLLMEVQVLRRLYETIFVFNYSPSARMHIFGYLTGFFFYTAAPLSLCSNCLPEVYHFAADGLAQFVFKSKMQMPDVEVSLLDVVIPLTNLGWRQWIGAALFFWGWIHQQRCHLILGSLRVRGGRTEEYRIPHGDWFEIVSSPHYLAEIVIYAGFVVASGGEDFTIWLLFGFVVANLAFAAAETHRWYFRKFDNYPRNRFAVIPYIF
Homology
BLAST of Lag0001513 vs. NCBI nr
Match: XP_004148545.1 (polyprenol reductase 2 [Cucumis sativus] >KGN43186.1 hypothetical protein Csa_020422 [Cucumis sativus])

HSP 1 Score: 619.0 bits (1595), Expect = 2.4e-173
Identity = 295/338 (87.28%), Postives = 313/338 (92.60%), Query Frame = 0

Query: 1   MELGIVWLLRAAWIAAILPILVAFLPFSRLNWFREIVLGFAKRGKILQSSSQKFTVPQKF 60
           MELGIVWLLRAAW A ILPI++AFLPFSRLNWFR I+LGFAKRGKILQSSSQKFTVPQKF
Sbjct: 1   MELGIVWLLRAAWFAGILPIIIAFLPFSRLNWFRAILLGFAKRGKILQSSSQKFTVPQKF 60

Query: 61  FCHFYVFAVIWTTLLLGTSWIYAYVSTPEIPEPYNFPAITSQLTGGSSLFSWQKSHSSRR 120
           FCHFYV AVIWTTLLLGT+W YAYVSTPEI EP+NFP ITSQLTGGSSLF WQKSHSSRR
Sbjct: 61  FCHFYVLAVIWTTLLLGTTWTYAYVSTPEISEPFNFPGITSQLTGGSSLFPWQKSHSSRR 120

Query: 121 EQTFLVWKAVFLLLLMEVQVLRRLYETIFVFNYSPSARMHIFGYLTGFFFYTAAPLSLCS 180
           E  FLVWKAVFLLLLMEVQVLRRLYETI+VFNYSPSARMHIFGYLTG FFYTAAPLSLCS
Sbjct: 121 EHGFLVWKAVFLLLLMEVQVLRRLYETIYVFNYSPSARMHIFGYLTGLFFYTAAPLSLCS 180

Query: 181 NCLPEVYHFAADGLAQFVFKSKMQMPDVEVSLLDVVIPLTNLGWRQWIGAALFFWGWIHQ 240
           +C+PEVYHFAA GLAQF+ + K  MPDVE+S LD VIPL+ LGWRQWIGAALFFWGWIHQ
Sbjct: 181 SCIPEVYHFAASGLAQFIVQGKRPMPDVEISPLDAVIPLSYLGWRQWIGAALFFWGWIHQ 240

Query: 241 QRCHLILGSLRVRGGRTEEYRIPHGDWFEIVSSPHYLAEIVIYAGFVVASGGEDFTIWLL 300
           QRCH ILGSLRVR  ++EEYRIPHGDWFE+VSSPHYLAEIVIY G VVASGGEDFTIWLL
Sbjct: 241 QRCHQILGSLRVRREQSEEYRIPHGDWFEVVSSPHYLAEIVIYGGLVVASGGEDFTIWLL 300

Query: 301 FGFVVANLAFAAAETHRWYFRKFDNYPRNRFAVIPYIF 339
           FGFVV NLAFAAAETHRWYFRKFDNYPRNRFA+IPY++
Sbjct: 301 FGFVVVNLAFAAAETHRWYFRKFDNYPRNRFAIIPYVY 338

BLAST of Lag0001513 vs. NCBI nr
Match: XP_038888381.1 (polyprenol reductase 2 isoform X2 [Benincasa hispida])

HSP 1 Score: 607.8 bits (1566), Expect = 5.6e-170
Identity = 294/338 (86.98%), Postives = 311/338 (92.01%), Query Frame = 0

Query: 1   MELGIVWLLRAAWIAAILPILVAFLPFSRLNWFREIVLGFAKRGKILQSSSQKFTVPQKF 60
           MELGIVWLLRAAWIA ILP++VAFLPFSRLNWFR I+LGFAKRGKILQSSS+KFTVPQKF
Sbjct: 1   MELGIVWLLRAAWIAGILPVVVAFLPFSRLNWFRGILLGFAKRGKILQSSSKKFTVPQKF 60

Query: 61  FCHFYVFAVIWTTLLLGTSWIYAYVSTPEIPEPYNFPAITSQLTGGSSLFSWQKSHSSRR 120
           FCHFYV AVIWTTLLLGT+W+YAYVSTPEI EP+NFPAITSQLTGGSSLFSWQKSHSSRR
Sbjct: 61  FCHFYVLAVIWTTLLLGTTWMYAYVSTPEISEPFNFPAITSQLTGGSSLFSWQKSHSSRR 120

Query: 121 EQTFLVWKAVFLLLLMEVQVLRRLYETIFVFNYSPSARMHIFGYLTGFFFYTAAPLSLCS 180
           E  FLVWKAVFLLLLMEVQVLRRLYETI+VF YS SARMHI GYLTG FFYTAAPLSLCS
Sbjct: 121 EHRFLVWKAVFLLLLMEVQVLRRLYETIYVFKYSLSARMHILGYLTGLFFYTAAPLSLCS 180

Query: 181 NCLPEVYHFAADGLAQFVFKSKMQMPDVEVSLLDVVIPLTNLGWRQWIGAALFFWGWIHQ 240
           NC+PEVYHFAA GLAQFV + K  MPDVEVSLLD VIPL+NLGW QWIGAALFFWGWIHQ
Sbjct: 181 NCIPEVYHFAASGLAQFVVQGKRHMPDVEVSLLDAVIPLSNLGWCQWIGAALFFWGWIHQ 240

Query: 241 QRCHLILGSLRVRGGRTEEYRIPHGDWFEIVSSPHYLAEIVIYAGFVVASGGEDFTIWLL 300
            RCH ILGSLRVR  ++EEYRIPHGDWF IVSSPHYLAE+VIY G VVASGGED TIWLL
Sbjct: 241 CRCHQILGSLRVRREQSEEYRIPHGDWFGIVSSPHYLAEMVIYGGLVVASGGEDITIWLL 300

Query: 301 FGFVVANLAFAAAETHRWYFRKFDNYPRNRFAVIPYIF 339
           FGFVV NLAFAAAETHRWYF KF+NYPRNRFA+IPYI+
Sbjct: 301 FGFVVVNLAFAAAETHRWYFHKFNNYPRNRFAIIPYIY 338

BLAST of Lag0001513 vs. NCBI nr
Match: XP_008444886.1 (PREDICTED: polyprenol reductase 2 [Cucumis melo])

HSP 1 Score: 606.7 bits (1563), Expect = 1.2e-169
Identity = 289/338 (85.50%), Postives = 309/338 (91.42%), Query Frame = 0

Query: 1   MELGIVWLLRAAWIAAILPILVAFLPFSRLNWFREIVLGFAKRGKILQSSSQKFTVPQKF 60
           MELGIVWLLRAAW+A ILPI+VAFLPFS+LNWFR I+L FAKRGKILQSSSQKFTVPQKF
Sbjct: 1   MELGIVWLLRAAWVAGILPIIVAFLPFSKLNWFRGILLRFAKRGKILQSSSQKFTVPQKF 60

Query: 61  FCHFYVFAVIWTTLLLGTSWIYAYVSTPEIPEPYNFPAITSQLTGGSSLFSWQKSHSSRR 120
           FCHFYV AVIWTT LLGT+WIYAYVSTPEI EP+NFPAITSQLTGGSSLF WQKSHSS R
Sbjct: 61  FCHFYVLAVIWTTFLLGTTWIYAYVSTPEISEPFNFPAITSQLTGGSSLFPWQKSHSSWR 120

Query: 121 EQTFLVWKAVFLLLLMEVQVLRRLYETIFVFNYSPSARMHIFGYLTGFFFYTAAPLSLCS 180
           E  FLVWKAVFL+LLMEVQVLRRLYETI+VFNYSPSARMHIFGYLTG FFYTAAPLSLCS
Sbjct: 121 EHGFLVWKAVFLILLMEVQVLRRLYETIYVFNYSPSARMHIFGYLTGLFFYTAAPLSLCS 180

Query: 181 NCLPEVYHFAADGLAQFVFKSKMQMPDVEVSLLDVVIPLTNLGWRQWIGAALFFWGWIHQ 240
           NC+PEVYHFAA GLAQF+ + +  MPDVE+S LD VIPL+NLGW QWIGA LFFWGWIHQ
Sbjct: 181 NCIPEVYHFAASGLAQFIVQGRRHMPDVEISPLDAVIPLSNLGWCQWIGATLFFWGWIHQ 240

Query: 241 QRCHLILGSLRVRGGRTEEYRIPHGDWFEIVSSPHYLAEIVIYAGFVVASGGEDFTIWLL 300
            RCH ILGSLRVR  ++EEYRIPHGDWFE+VSSPHYLAEIVIY G VVASGG+D TIWLL
Sbjct: 241 HRCHQILGSLRVRREQSEEYRIPHGDWFEVVSSPHYLAEIVIYGGLVVASGGKDLTIWLL 300

Query: 301 FGFVVANLAFAAAETHRWYFRKFDNYPRNRFAVIPYIF 339
           FGFVV NLAF AAETHRWYFRKFDNYPRNRFA+IPYI+
Sbjct: 301 FGFVVVNLAFVAAETHRWYFRKFDNYPRNRFAIIPYIY 338

BLAST of Lag0001513 vs. NCBI nr
Match: KAG7022255.1 (Polyprenol reductase 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 592.8 bits (1527), Expect = 1.9e-165
Identity = 283/338 (83.73%), Postives = 307/338 (90.83%), Query Frame = 0

Query: 1   MELGIVWLLRAAWIAAILPILVAFLPFSRLNWFREIVLGFAKRGKILQSSSQKFTVPQKF 60
           ME+GIVWL+RAAWIA ILP+L+A LPFSRL+W+R  +L FAKRGKILQSSS+KFTVPQKF
Sbjct: 1   MEIGIVWLIRAAWIAGILPLLLALLPFSRLSWYRRTILEFAKRGKILQSSSKKFTVPQKF 60

Query: 61  FCHFYVFAVIWTTLLLGTSWIYAYVSTPEIPEPYNFPAITSQLTGGSSLFSWQKSHSSRR 120
           FCHFYV+AVIWT LLLGT+W+YAYVSTP I EP+NFPAITSQLTGGSSLFSWQKSHSS R
Sbjct: 61  FCHFYVWAVIWTALLLGTTWMYAYVSTPLISEPFNFPAITSQLTGGSSLFSWQKSHSSGR 120

Query: 121 EQTFLVWKAVFLLLLMEVQVLRRLYETIFVFNYSPSARMHIFGYLTGFFFYTAAPLSLCS 180
           E+ FLVWKAVFLLLLMEVQVLRRLYETI+ FNYSPSARMH FGYLTG FFYTAAPLSLCS
Sbjct: 121 ERRFLVWKAVFLLLLMEVQVLRRLYETIYTFNYSPSARMHFFGYLTGLFFYTAAPLSLCS 180

Query: 181 NCLPEVYHFAADGLAQFVFKSKMQMPDVEVSLLDVVIPLTNLGWRQWIGAALFFWGWIHQ 240
           NC+PEVYHFAA+G+AQF+ K K QM DVEVSLLD VIPL NLGWRQWIGAALFFWGWIHQ
Sbjct: 181 NCIPEVYHFAANGIAQFIVKGKGQMLDVEVSLLDAVIPLANLGWRQWIGAALFFWGWIHQ 240

Query: 241 QRCHLILGSLRVRGGRTEEYRIPHGDWFEIVSSPHYLAEIVIYAGFVVASGGEDFTIWLL 300
            RCH IL SLRV G + EEY+IPHGDWFEIVSSPHYL+EIVIY G VVASGGED TIWL+
Sbjct: 241 FRCHQILASLRVPGEQGEEYKIPHGDWFEIVSSPHYLSEIVIYGGLVVASGGEDLTIWLI 300

Query: 301 FGFVVANLAFAAAETHRWYFRKFDNYPRNRFAVIPYIF 339
           FGFVV NLA AAAETHRWY RKFDNYPRNRFA+IPYI+
Sbjct: 301 FGFVVVNLALAAAETHRWYVRKFDNYPRNRFAIIPYIY 338

BLAST of Lag0001513 vs. NCBI nr
Match: XP_023530646.1 (polyprenol reductase 2-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 592.0 bits (1525), Expect = 3.2e-165
Identity = 285/337 (84.57%), Postives = 305/337 (90.50%), Query Frame = 0

Query: 1   MELGIVWLLRAAWIAAILPILVAFLPFSRLNWFREIVLGFAKRGKILQSSSQKFTVPQKF 60
           ME+GIVWLLRAAWIA ILPIL+A LPFSRL+W+R  +L FAKRGKILQSSS+KFTVPQKF
Sbjct: 1   MEIGIVWLLRAAWIAGILPILLALLPFSRLSWYRRTILEFAKRGKILQSSSKKFTVPQKF 60

Query: 61  FCHFYVFAVIWTTLLLGTSWIYAYVSTPEIPEPYNFPAITSQLTGGSSLFSWQKSHSSRR 120
           FCHFYV+AVIWT LLLGT+W+YAYVSTP I EP+NFPAITSQLTGGSSLFSWQKSHSSRR
Sbjct: 61  FCHFYVWAVIWTALLLGTTWMYAYVSTPLISEPFNFPAITSQLTGGSSLFSWQKSHSSRR 120

Query: 121 EQTFLVWKAVFLLLLMEVQVLRRLYETIFVFNYSPSARMHIFGYLTGFFFYTAAPLSLCS 180
           E  FLVWKAVFLLLLMEVQVLRRLYETI+ FNYSPSARMH FGYLTGFFFYTAAPLSLCS
Sbjct: 121 EHRFLVWKAVFLLLLMEVQVLRRLYETIYTFNYSPSARMHFFGYLTGFFFYTAAPLSLCS 180

Query: 181 NCLPEVYHFAADGLAQFVFKSKMQMPDVEVSLLDVVIPLTNLGWRQWIGAALFFWGWIHQ 240
           NC+PEVYHFAA+G+AQF+ + K QM  VEVSLLD VIPL NLGWRQWIGAALFFWGWIHQ
Sbjct: 181 NCIPEVYHFAANGIAQFIVEGKGQMLVVEVSLLDAVIPLANLGWRQWIGAALFFWGWIHQ 240

Query: 241 QRCHLILGSLRVRGGRTEEYRIPHGDWFEIVSSPHYLAEIVIYAGFVVASGGEDFTIWLL 300
            RCH IL SLRV G +  EY+IPHGDWFEIVSSPHYLAEIVIY G VVASGGED TIWL+
Sbjct: 241 FRCHQILASLRVPGEQGAEYKIPHGDWFEIVSSPHYLAEIVIYGGLVVASGGEDLTIWLI 300

Query: 301 FGFVVANLAFAAAETHRWYFRKFDNYPRNRFAVIPYI 338
           FGFVV NLA AAAETHRWY RKFDNYPRNRFA+IPYI
Sbjct: 301 FGFVVVNLALAAAETHRWYVRKFDNYPRNRFAIIPYI 337

BLAST of Lag0001513 vs. ExPASy Swiss-Prot
Match: Q9SI62 (Polyprenol reductase 2 OS=Arabidopsis thaliana OX=3702 GN=PPRD2 PE=1 SV=2)

HSP 1 Score: 413.3 bits (1061), Expect = 2.6e-114
Identity = 202/342 (59.06%), Postives = 245/342 (71.64%), Query Frame = 0

Query: 1   MELGIVWLLRAA----WIAAILPILVAFLPFSRLNWFREIVLGFAKRGKILQSSSQKFTV 60
           +EL IVWL+R A    WI +ILP+++A +P S+LN FRE+VL FA RGKIL  SSQKFT+
Sbjct: 2   VELEIVWLVRGAWITVWIVSILPLVIASIPTSKLNSFRELVLSFAGRGKILHPSSQKFTI 61

Query: 61  PQKFFCHFYVFAVIWTTLLLGTSWIYAYVSTPEIPEPYNFPAITSQLTGGSSLFSWQKSH 120
           PQK F HFYV  V+WTTLLL  +W+YA    P   E +    I S+L GGS +FS  KS+
Sbjct: 62  PQKCFAHFYVIGVVWTTLLLAATWMYACKMAPLSSEEFQLSDIASRLAGGSDVFSVHKSN 121

Query: 121 SSRREQTFLVWKAVFLLLLMEVQVLRRLYETIFVFNYSPSARMHIFGYLTGFFFYTAAPL 180
            +  E  F VW+AVFLLLLME+ VLRRL E+ +VF YSPSARMHI GY  G FFY  APL
Sbjct: 122 MTPVEHRFKVWRAVFLLLLMEIHVLRRLIESFYVFKYSPSARMHILGYFAGLFFYVTAPL 181

Query: 181 SLCSNCLPEVYHFAADGLAQFVFKSKMQMPDVEVSLLDVVIPLTNLGWRQWIGAALFFWG 240
           SLCSN  PEV  F  + +A+F+   K      E +LL  + PL  LG  QWIG A+F WG
Sbjct: 182 SLCSNIAPEVAGFVGNQVAEFIANGKSHTSAPEFNLLSSISPLMKLGSLQWIGGAIFLWG 241

Query: 241 WIHQQRCHLILGSLRVRGGRTEEYRIPHGDWFEIVSSPHYLAEIVIYAGFVVASGGEDFT 300
           WIHQ+RCH ILGSLR    + +EY IP+GDWF +VSSPH+LAEIV+YAG ++ASGG D T
Sbjct: 242 WIHQRRCHAILGSLRENPSQAKEYIIPYGDWFGMVSSPHFLAEIVLYAGLLIASGGTDIT 301

Query: 301 IWLLFGFVVANLAFAAAETHRWYFRKFDNYPRNRFAVIPYIF 339
           IWLLFGFV ANL +AA ETHRWY RKF+NYP NR A+ PY++
Sbjct: 302 IWLLFGFVAANLTYAAGETHRWYLRKFENYPANRHAIFPYVY 343

BLAST of Lag0001513 vs. ExPASy Swiss-Prot
Match: Q9CAH5 (Polyprenol reductase 1 OS=Arabidopsis thaliana OX=3702 GN=PPRD1 PE=1 SV=1)

HSP 1 Score: 368.6 bits (945), Expect = 7.4e-101
Identity = 187/342 (54.68%), Postives = 237/342 (69.30%), Query Frame = 0

Query: 1   MELGIVWLLRAA----WIAAILPILVAFLPFSRLNWFREIVLGFAKRGKILQSSSQKFTV 60
           ME+ IVWL++AA    WI +ILP+++A +P S+LN FRE+VL FA RGKIL  SSQKFTV
Sbjct: 1   MEVEIVWLVKAAWITVWIVSILPLVIASIPSSKLNSFRELVLSFAGRGKILHPSSQKFTV 60

Query: 61  PQKFFCHFYVFAVIWTTLLLGTSWIYAYVSTPEIPEPYNFPAITSQLTGGSSLFSWQKSH 120
           PQKFF HFYV  V+WTTLLL  +W+YA                  ++ GGS +FS+  +H
Sbjct: 61  PQKFFGHFYVVGVVWTTLLLAATWMYA-----------------CKMAGGSHVFSFHMTH 120

Query: 121 SSRREQTFLVWKAVFLLLLMEVQVLRRLYETIFVFNYSPSARMHIFGYLTGFFFYTAAPL 180
              R   F V +AVFLLLLME+ VLRR+ E+ +VF YS SARMHI  Y+   F+Y AAPL
Sbjct: 121 VEHR---FKVGRAVFLLLLMEIHVLRRVIESFYVFKYSTSARMHILAYVGALFYYVAAPL 180

Query: 181 SLCSNCLPEVYHFAADGLAQFVFKSKMQMPDVEVSLLDVVIPLTNLGWRQWIGAALFFWG 240
           SLCSN  PEV  F    +A+F+   K    D   +LL  + PL  LG  QWIG A+F WG
Sbjct: 181 SLCSNIAPEVARFVGSQVAEFIASGKSHSHD--FNLLLSISPLMKLGSLQWIGGAIFLWG 240

Query: 241 WIHQQRCHLILGSLRVRGGRTEEYRIPHGDWFEIVSSPHYLAEIVIYAGFVVASGGEDFT 300
           WIHQ+RCH ILGSLR    + +EY IP+GDWFE+VS PH+LAEIV+Y G +++SGG D +
Sbjct: 241 WIHQRRCHAILGSLREYPSQAKEYIIPYGDWFEMVSCPHFLAEIVLYLGLLISSGGTDIS 300

Query: 301 IWLLFGFVVANLAFAAAETHRWYFRKFDNYPRNRFAVIPYIF 339
           IWLLFGFV ANL +AA ETHRWY +KF+NYP +R A+ P+++
Sbjct: 301 IWLLFGFVAANLTYAAGETHRWYLQKFENYPASRHAIFPHVY 320

BLAST of Lag0001513 vs. ExPASy Swiss-Prot
Match: A2XWN6 (Polyprenol reductase 1 OS=Oryza sativa subsp. indica OX=39946 GN=OsI_17065 PE=3 SV=1)

HSP 1 Score: 352.4 bits (903), Expect = 5.5e-96
Identity = 180/344 (52.33%), Postives = 234/344 (68.02%), Query Frame = 0

Query: 8   LLRAAWIAAILPILVAFLPFSRL---NWFREIVLGFAKRGKILQ----------SSSQKF 67
           LL  AWIA  LPI+VA LP       +  R ++  F+ RGK ++          SS  KF
Sbjct: 12  LLCLAWIATTLPIIVAALPIPAAAGGHLLRRLLSAFSSRGKTVRPSPASSSGSSSSKAKF 71

Query: 68  TVPQKFFCHFYVFAVIWTTLLLGTSWIYAYVS-TPEIPEPYNFPAITSQLTGGSSLFSWQ 127
           TVPQK+F HFYV  V+ TT+LL   W YAY+  TP +PE  ++  I S L G +S FS+ 
Sbjct: 72  TVPQKYFMHFYVVGVLATTILLLAIWFYAYMKMTPLLPESSSYSTIASHLVGSNS-FSFG 131

Query: 128 KSHSSRREQTFLVWKAVFLLLLMEVQVLRRLYETIFVFNYSPSARMHIFGYLTGFFFYTA 187
           + HS      + VW+ VF+LLLME+QVLRRLYET  VF+YSPSARMHI GYLTG F+Y A
Sbjct: 132 RVHSRTMGHKYHVWRTVFVLLLMEIQVLRRLYETEHVFHYSPSARMHIVGYLTGLFYYVA 191

Query: 188 APLSLCSNCLPEVYHFAADGLAQFVFKSKMQMPDVEVSLLDVVIPLTNLGWRQWIGAALF 247
           APLSL S+C+PE   +    +A+F+ K + +MPD+ +    ++ PL  LGW QWIGA +F
Sbjct: 192 APLSLASSCIPEAAEYLQGQVAEFIVKGRARMPDLVIDSSSLLQPLLKLGWTQWIGAVIF 251

Query: 248 FWGWIHQQRCHLILGSLRVRGGRTEEYRIPHGDWFEIVSSPHYLAEIVIYAGFVVASGGE 307
            WG +HQ RCH ILG+LR     ++EY IP GDWF  VS PHYLAE+VIY G +VASGGE
Sbjct: 252 IWGSLHQIRCHAILGTLREHKD-SDEYVIPCGDWFNRVSCPHYLAELVIYFGMLVASGGE 311

Query: 308 DFTIWLLFGFVVANLAFAAAETHRWYFRKFDNYPRNRFAVIPYI 338
           D  +W LF FV+ NL+FAA ETH+WY +KF++YPR+R+A+IP++
Sbjct: 312 DIPVWFLFVFVITNLSFAAVETHKWYLQKFEDYPRSRYAIIPFV 353

BLAST of Lag0001513 vs. ExPASy Swiss-Prot
Match: Q7XUH5 (Polyprenol reductase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0576800 PE=2 SV=2)

HSP 1 Score: 352.4 bits (903), Expect = 5.5e-96
Identity = 180/344 (52.33%), Postives = 234/344 (68.02%), Query Frame = 0

Query: 8   LLRAAWIAAILPILVAFLPFSRL---NWFREIVLGFAKRGKILQ----------SSSQKF 67
           LL  AWIA  LPI+VA LP       +  R ++  F+ RGK ++          SS  KF
Sbjct: 12  LLCLAWIATTLPIIVAALPIPAAAGGHLLRRLLSAFSSRGKTVRPSPASSSGSSSSKAKF 71

Query: 68  TVPQKFFCHFYVFAVIWTTLLLGTSWIYAYVS-TPEIPEPYNFPAITSQLTGGSSLFSWQ 127
           TVPQK+F HFYV  V+ TT+LL   W YAY+  TP +PE  ++  I S L G +S FS+ 
Sbjct: 72  TVPQKYFMHFYVVGVLATTILLLAIWFYAYMKMTPLLPESSSYSTIASHLVGSNS-FSFG 131

Query: 128 KSHSSRREQTFLVWKAVFLLLLMEVQVLRRLYETIFVFNYSPSARMHIFGYLTGFFFYTA 187
           + HS      + VW+ VF+LLLME+QVLRRLYET  VF+YSPSARMHI GYLTG F+Y A
Sbjct: 132 RVHSRTMGHKYHVWRTVFVLLLMEIQVLRRLYETEHVFHYSPSARMHIVGYLTGLFYYVA 191

Query: 188 APLSLCSNCLPEVYHFAADGLAQFVFKSKMQMPDVEVSLLDVVIPLTNLGWRQWIGAALF 247
           APLSL S+C+PE   +    +A+F+ K + +MPD+ +    ++ PL  LGW QWIGA +F
Sbjct: 192 APLSLASSCIPEAAEYLQGQVAEFIVKGRARMPDLVIDSSSLLQPLLKLGWTQWIGAVIF 251

Query: 248 FWGWIHQQRCHLILGSLRVRGGRTEEYRIPHGDWFEIVSSPHYLAEIVIYAGFVVASGGE 307
            WG +HQ RCH ILG+LR     ++EY IP GDWF  VS PHYLAE+VIY G +VASGGE
Sbjct: 252 IWGSLHQIRCHAILGTLREHKD-SDEYVIPCGDWFNRVSCPHYLAELVIYFGMLVASGGE 311

Query: 308 DFTIWLLFGFVVANLAFAAAETHRWYFRKFDNYPRNRFAVIPYI 338
           D  +W LF FV+ NL+FAA ETH+WY +KF++YPR+R+A+IP++
Sbjct: 312 DIPVWFLFVFVITNLSFAAVETHKWYLQKFEDYPRSRYAIIPFV 353

BLAST of Lag0001513 vs. ExPASy Swiss-Prot
Match: Q7F0Q2 (Polyprenol reductase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os07g0493400 PE=2 SV=1)

HSP 1 Score: 325.1 bits (832), Expect = 9.4e-88
Identity = 176/344 (51.16%), Postives = 225/344 (65.41%), Query Frame = 0

Query: 8   LLRAAWIAAILPILVAFLPFSRL---NWFREIVLGFAKRGKILQ----------SSSQKF 67
           LL  AWIAA LPI+ A LP       +  R ++  F+ RGK ++          SS  KF
Sbjct: 12  LLCFAWIAATLPIIAAALPIPTAVGGHLLRRLLSAFSSRGKTVRPSPASSSGSSSSKAKF 71

Query: 68  TVPQKFFCHFYVFAVIWTTLLLGTSWIYAYVS-TPEIPEPYNFPAITSQLTGGSSLFSWQ 127
           TVPQK+F HFYV  V+ TT+LL   W YAY+  TP + E  ++  I S L G +S FS+ 
Sbjct: 72  TVPQKYFMHFYVVGVLATTILLLAIWFYAYMKLTPLLLESSSYSTIFSHLVGSNS-FSFG 131

Query: 128 KSHSSRREQTFLVWKAVFLLLLMEVQVLRRLYETIFVFNYSPSARMHIFGYLTGFFFYTA 187
           +  S      + VW+ VF LLLMEVQVLRRLYET  VF+YSP ARMHI GYLTG F+Y A
Sbjct: 132 RVRSRTMGHKYRVWRTVFALLLMEVQVLRRLYETEHVFHYSP-ARMHIVGYLTGLFYYVA 191

Query: 188 APLSLCSNCLPEVYHFAADGLAQFVFKSKMQMPDVEVSLLDVVIPLTNLGWRQWIGAALF 247
           APLSL S+C+PE   +    + +FV K + +MPD+ +    ++ PL  LGW QWIGA +F
Sbjct: 192 APLSLASSCIPEAAEYFQGQVPEFVVKGRARMPDLVIDSSSLLQPLLKLGWTQWIGAVIF 251

Query: 248 FWGWIHQQRCHLILGSLRVRGGRTEEYRIPHGDWFEIVSSPHYLAEIVIYAGFVVASGGE 307
            WG +HQ RCH ILGSLR      +EY IP GD F  VS PHYLAE+VIY G +VASG E
Sbjct: 252 IWGSLHQIRCHAILGSLREHKD-YDEYVIPCGDCFNRVSCPHYLAELVIYFGMLVASGAE 311

Query: 308 DFTIWLLFGFVVANLAFAAAETHRWYFRKFDNYPRNRFAVIPYI 338
           D  +W LF FV+ NL+FAA ET+ WY +KF++YPR+R+A+IP++
Sbjct: 312 DIPVWFLFIFVITNLSFAAVETYNWYLQKFEDYPRSRYAIIPFV 352

BLAST of Lag0001513 vs. ExPASy TrEMBL
Match: A0A0A0K5R7 (Polyprenol reductase OS=Cucumis sativus OX=3659 GN=Csa_7G007840 PE=3 SV=1)

HSP 1 Score: 619.0 bits (1595), Expect = 1.2e-173
Identity = 295/338 (87.28%), Postives = 313/338 (92.60%), Query Frame = 0

Query: 1   MELGIVWLLRAAWIAAILPILVAFLPFSRLNWFREIVLGFAKRGKILQSSSQKFTVPQKF 60
           MELGIVWLLRAAW A ILPI++AFLPFSRLNWFR I+LGFAKRGKILQSSSQKFTVPQKF
Sbjct: 1   MELGIVWLLRAAWFAGILPIIIAFLPFSRLNWFRAILLGFAKRGKILQSSSQKFTVPQKF 60

Query: 61  FCHFYVFAVIWTTLLLGTSWIYAYVSTPEIPEPYNFPAITSQLTGGSSLFSWQKSHSSRR 120
           FCHFYV AVIWTTLLLGT+W YAYVSTPEI EP+NFP ITSQLTGGSSLF WQKSHSSRR
Sbjct: 61  FCHFYVLAVIWTTLLLGTTWTYAYVSTPEISEPFNFPGITSQLTGGSSLFPWQKSHSSRR 120

Query: 121 EQTFLVWKAVFLLLLMEVQVLRRLYETIFVFNYSPSARMHIFGYLTGFFFYTAAPLSLCS 180
           E  FLVWKAVFLLLLMEVQVLRRLYETI+VFNYSPSARMHIFGYLTG FFYTAAPLSLCS
Sbjct: 121 EHGFLVWKAVFLLLLMEVQVLRRLYETIYVFNYSPSARMHIFGYLTGLFFYTAAPLSLCS 180

Query: 181 NCLPEVYHFAADGLAQFVFKSKMQMPDVEVSLLDVVIPLTNLGWRQWIGAALFFWGWIHQ 240
           +C+PEVYHFAA GLAQF+ + K  MPDVE+S LD VIPL+ LGWRQWIGAALFFWGWIHQ
Sbjct: 181 SCIPEVYHFAASGLAQFIVQGKRPMPDVEISPLDAVIPLSYLGWRQWIGAALFFWGWIHQ 240

Query: 241 QRCHLILGSLRVRGGRTEEYRIPHGDWFEIVSSPHYLAEIVIYAGFVVASGGEDFTIWLL 300
           QRCH ILGSLRVR  ++EEYRIPHGDWFE+VSSPHYLAEIVIY G VVASGGEDFTIWLL
Sbjct: 241 QRCHQILGSLRVRREQSEEYRIPHGDWFEVVSSPHYLAEIVIYGGLVVASGGEDFTIWLL 300

Query: 301 FGFVVANLAFAAAETHRWYFRKFDNYPRNRFAVIPYIF 339
           FGFVV NLAFAAAETHRWYFRKFDNYPRNRFA+IPY++
Sbjct: 301 FGFVVVNLAFAAAETHRWYFRKFDNYPRNRFAIIPYVY 338

BLAST of Lag0001513 vs. ExPASy TrEMBL
Match: A0A1S3BBF0 (Polyprenol reductase OS=Cucumis melo OX=3656 GN=LOC103488097 PE=3 SV=1)

HSP 1 Score: 606.7 bits (1563), Expect = 6.0e-170
Identity = 289/338 (85.50%), Postives = 309/338 (91.42%), Query Frame = 0

Query: 1   MELGIVWLLRAAWIAAILPILVAFLPFSRLNWFREIVLGFAKRGKILQSSSQKFTVPQKF 60
           MELGIVWLLRAAW+A ILPI+VAFLPFS+LNWFR I+L FAKRGKILQSSSQKFTVPQKF
Sbjct: 1   MELGIVWLLRAAWVAGILPIIVAFLPFSKLNWFRGILLRFAKRGKILQSSSQKFTVPQKF 60

Query: 61  FCHFYVFAVIWTTLLLGTSWIYAYVSTPEIPEPYNFPAITSQLTGGSSLFSWQKSHSSRR 120
           FCHFYV AVIWTT LLGT+WIYAYVSTPEI EP+NFPAITSQLTGGSSLF WQKSHSS R
Sbjct: 61  FCHFYVLAVIWTTFLLGTTWIYAYVSTPEISEPFNFPAITSQLTGGSSLFPWQKSHSSWR 120

Query: 121 EQTFLVWKAVFLLLLMEVQVLRRLYETIFVFNYSPSARMHIFGYLTGFFFYTAAPLSLCS 180
           E  FLVWKAVFL+LLMEVQVLRRLYETI+VFNYSPSARMHIFGYLTG FFYTAAPLSLCS
Sbjct: 121 EHGFLVWKAVFLILLMEVQVLRRLYETIYVFNYSPSARMHIFGYLTGLFFYTAAPLSLCS 180

Query: 181 NCLPEVYHFAADGLAQFVFKSKMQMPDVEVSLLDVVIPLTNLGWRQWIGAALFFWGWIHQ 240
           NC+PEVYHFAA GLAQF+ + +  MPDVE+S LD VIPL+NLGW QWIGA LFFWGWIHQ
Sbjct: 181 NCIPEVYHFAASGLAQFIVQGRRHMPDVEISPLDAVIPLSNLGWCQWIGATLFFWGWIHQ 240

Query: 241 QRCHLILGSLRVRGGRTEEYRIPHGDWFEIVSSPHYLAEIVIYAGFVVASGGEDFTIWLL 300
            RCH ILGSLRVR  ++EEYRIPHGDWFE+VSSPHYLAEIVIY G VVASGG+D TIWLL
Sbjct: 241 HRCHQILGSLRVRREQSEEYRIPHGDWFEVVSSPHYLAEIVIYGGLVVASGGKDLTIWLL 300

Query: 301 FGFVVANLAFAAAETHRWYFRKFDNYPRNRFAVIPYIF 339
           FGFVV NLAF AAETHRWYFRKFDNYPRNRFA+IPYI+
Sbjct: 301 FGFVVVNLAFVAAETHRWYFRKFDNYPRNRFAIIPYIY 338

BLAST of Lag0001513 vs. ExPASy TrEMBL
Match: A0A6J1I5R2 (Polyprenol reductase OS=Cucurbita maxima OX=3661 GN=LOC111469837 PE=3 SV=1)

HSP 1 Score: 591.3 bits (1523), Expect = 2.6e-165
Identity = 283/338 (83.73%), Postives = 305/338 (90.24%), Query Frame = 0

Query: 1   MELGIVWLLRAAWIAAILPILVAFLPFSRLNWFREIVLGFAKRGKILQSSSQKFTVPQKF 60
           ME+GIVWLLRAAWIA ILPIL+A LPFSRL+W+R  +L FAKRGKILQSS++KFTVPQKF
Sbjct: 1   MEIGIVWLLRAAWIAGILPILLALLPFSRLSWYRRTILEFAKRGKILQSSTKKFTVPQKF 60

Query: 61  FCHFYVFAVIWTTLLLGTSWIYAYVSTPEIPEPYNFPAITSQLTGGSSLFSWQKSHSSRR 120
           FCHFYV+AVIWT LLLGT+W+YAYVSTP I EP+NFPAITSQLTGGSSLFSWQKSHSSRR
Sbjct: 61  FCHFYVWAVIWTALLLGTTWMYAYVSTPLISEPFNFPAITSQLTGGSSLFSWQKSHSSRR 120

Query: 121 EQTFLVWKAVFLLLLMEVQVLRRLYETIFVFNYSPSARMHIFGYLTGFFFYTAAPLSLCS 180
           E  FLVWKAVFLLLLMEVQVLRRLYETI+ FNYSPSARMH FGYLTG FFYTAAPLSLCS
Sbjct: 121 EHRFLVWKAVFLLLLMEVQVLRRLYETIYTFNYSPSARMHFFGYLTGLFFYTAAPLSLCS 180

Query: 181 NCLPEVYHFAADGLAQFVFKSKMQMPDVEVSLLDVVIPLTNLGWRQWIGAALFFWGWIHQ 240
           NC+ EVYHFAA+G+AQF+ K K QM DVEVSLLD VIPL NLGWRQWIGAALFFWGWIHQ
Sbjct: 181 NCITEVYHFAANGIAQFIVKGKGQMLDVEVSLLDAVIPLANLGWRQWIGAALFFWGWIHQ 240

Query: 241 QRCHLILGSLRVRGGRTEEYRIPHGDWFEIVSSPHYLAEIVIYAGFVVASGGEDFTIWLL 300
             CH IL SLR+ G + EEY+IPHGDWFEIVSSPHYLAEIVIY G VVASGGED TIWL+
Sbjct: 241 CHCHQILASLRIPGEQGEEYKIPHGDWFEIVSSPHYLAEIVIYGGLVVASGGEDLTIWLI 300

Query: 301 FGFVVANLAFAAAETHRWYFRKFDNYPRNRFAVIPYIF 339
           FGFVV NLA AAAETHRWY RKFDNYPRNRFA+IPYI+
Sbjct: 301 FGFVVVNLALAAAETHRWYVRKFDNYPRNRFAIIPYIY 338

BLAST of Lag0001513 vs. ExPASy TrEMBL
Match: A0A6J1F6C0 (Polyprenol reductase OS=Cucurbita moschata OX=3662 GN=LOC111441221 PE=3 SV=1)

HSP 1 Score: 588.2 bits (1515), Expect = 2.2e-164
Identity = 282/338 (83.43%), Postives = 304/338 (89.94%), Query Frame = 0

Query: 1   MELGIVWLLRAAWIAAILPILVAFLPFSRLNWFREIVLGFAKRGKILQSSSQKFTVPQKF 60
           ME+GIVWL+RAAWIA ILPIL+A LPFSRL+W+R  +L FAKRGKILQSSS+KFTVPQKF
Sbjct: 1   MEIGIVWLIRAAWIAGILPILLALLPFSRLSWYRRTILEFAKRGKILQSSSKKFTVPQKF 60

Query: 61  FCHFYVFAVIWTTLLLGTSWIYAYVSTPEIPEPYNFPAITSQLTGGSSLFSWQKSHSSRR 120
           FCHFYV+AVIWT LLLGT+W+YAYVSTP I EP+NFPAITSQLTGGSSLFSWQKSHSS R
Sbjct: 61  FCHFYVWAVIWTALLLGTTWMYAYVSTPLISEPFNFPAITSQLTGGSSLFSWQKSHSSGR 120

Query: 121 EQTFLVWKAVFLLLLMEVQVLRRLYETIFVFNYSPSARMHIFGYLTGFFFYTAAPLSLCS 180
           E  FLVWKAVFLLLLMEVQVLRRLYETI+ F YSPSARMH FGYLTG FFYTAAPLSLCS
Sbjct: 121 EHRFLVWKAVFLLLLMEVQVLRRLYETIYTFIYSPSARMHFFGYLTGLFFYTAAPLSLCS 180

Query: 181 NCLPEVYHFAADGLAQFVFKSKMQMPDVEVSLLDVVIPLTNLGWRQWIGAALFFWGWIHQ 240
           NC+PEVYHFA +G+AQF+ K K QM DVEVSLLD VIPL NLGWRQWIGAALFFWGWIHQ
Sbjct: 181 NCIPEVYHFATNGIAQFIVKGKGQMLDVEVSLLDAVIPLANLGWRQWIGAALFFWGWIHQ 240

Query: 241 QRCHLILGSLRVRGGRTEEYRIPHGDWFEIVSSPHYLAEIVIYAGFVVASGGEDFTIWLL 300
            RCH IL SLRV G + EEY+IPHGDWFEIVSSPHYL+EIVIY G VVASGGED TIWL+
Sbjct: 241 FRCHQILASLRVPGEQGEEYKIPHGDWFEIVSSPHYLSEIVIYGGLVVASGGEDLTIWLI 300

Query: 301 FGFVVANLAFAAAETHRWYFRKFDNYPRNRFAVIPYIF 339
           FGFVV NLA AAAETHRWY RKFDNYPRNRFA+IPYI+
Sbjct: 301 FGFVVVNLALAAAETHRWYVRKFDNYPRNRFAIIPYIY 338

BLAST of Lag0001513 vs. ExPASy TrEMBL
Match: A0A6J1C196 (Polyprenol reductase OS=Momordica charantia OX=3673 GN=LOC111007527 PE=3 SV=1)

HSP 1 Score: 563.1 bits (1450), Expect = 7.6e-157
Identity = 276/338 (81.66%), Postives = 297/338 (87.87%), Query Frame = 0

Query: 1   MELGIVWLLRAAWIAAILPILVAFLPFSRLNWFREIVLGFAKRGKILQSSSQKFTVPQKF 60
           MELGI  LLRAAWIA  LPILVAFLPFS L+WFR ++LGFA+RGKILQSSSQKFTVPQK+
Sbjct: 1   MELGIDCLLRAAWIAVALPILVAFLPFSSLDWFRGVLLGFARRGKILQSSSQKFTVPQKY 60

Query: 61  FCHFYVFAVIWTTLLLGTSWIYAYVSTPEIPEPYNFPAITSQLTGGSSLFSWQKSHSSRR 120
           FCHFYV AVIWTTLLLGT+WIYAY  TPEI EP+NFPAITSQLTGGSSLFSW KS SSRR
Sbjct: 61  FCHFYVLAVIWTTLLLGTTWIYAYALTPEISEPFNFPAITSQLTGGSSLFSWGKSLSSRR 120

Query: 121 EQTFLVWKAVFLLLLMEVQVLRRLYETIFVFNYSPSARMHIFGYLTGFFFYTAAPLSLCS 180
           E  FLVW+ VFLLLLMEVQVLRRLYETI+VFNYS SARMHI GYLTGFFFYTAAPLSLCS
Sbjct: 121 ETRFLVWRTVFLLLLMEVQVLRRLYETIYVFNYSRSARMHICGYLTGFFFYTAAPLSLCS 180

Query: 181 NCLPEVYHFAADGLAQFVFKSKMQMPDVEVSLLDVVIPLTNLGWRQWIGAALFFWGWIHQ 240
           N +PEVYHFA +GL +F+ + K  MPDVEV   D VIPLTNLGW Q IGAALFFWGWIHQ
Sbjct: 181 NFIPEVYHFATNGLVKFIVEGKRHMPDVEV--FDFVIPLTNLGWHQCIGAALFFWGWIHQ 240

Query: 241 QRCHLILGSLRVRGGRTEEYRIPHGDWFEIVSSPHYLAEIVIYAGFVVASGGEDFTIWLL 300
             CH ILGSLRV G ++EEY+IPHGDWFEIVSSPHYLAEIVIYAG VVASGGED TIWL 
Sbjct: 241 HHCHQILGSLRVPGQQSEEYKIPHGDWFEIVSSPHYLAEIVIYAGLVVASGGEDLTIWLA 300

Query: 301 FGFVVANLAFAAAETHRWYFRKFDNYPRNRFAVIPYIF 339
           FGFVV NLAFAA +THRWY RKFD+YPRNRFA+IPYI+
Sbjct: 301 FGFVVVNLAFAAVDTHRWYLRKFDDYPRNRFAIIPYIY 336

BLAST of Lag0001513 vs. TAIR 10
Match: AT2G16530.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 413.3 bits (1061), Expect = 1.9e-115
Identity = 202/342 (59.06%), Postives = 245/342 (71.64%), Query Frame = 0

Query: 1   MELGIVWLLRAA----WIAAILPILVAFLPFSRLNWFREIVLGFAKRGKILQSSSQKFTV 60
           +EL IVWL+R A    WI +ILP+++A +P S+LN FRE+VL FA RGKIL  SSQKFT+
Sbjct: 2   VELEIVWLVRGAWITVWIVSILPLVIASIPTSKLNSFRELVLSFAGRGKILHPSSQKFTI 61

Query: 61  PQKFFCHFYVFAVIWTTLLLGTSWIYAYVSTPEIPEPYNFPAITSQLTGGSSLFSWQKSH 120
           PQK F HFYV  V+WTTLLL  +W+YA    P   E +    I S+L GGS +FS  KS+
Sbjct: 62  PQKCFAHFYVIGVVWTTLLLAATWMYACKMAPLSSEEFQLSDIASRLAGGSDVFSVHKSN 121

Query: 121 SSRREQTFLVWKAVFLLLLMEVQVLRRLYETIFVFNYSPSARMHIFGYLTGFFFYTAAPL 180
            +  E  F VW+AVFLLLLME+ VLRRL E+ +VF YSPSARMHI GY  G FFY  APL
Sbjct: 122 MTPVEHRFKVWRAVFLLLLMEIHVLRRLIESFYVFKYSPSARMHILGYFAGLFFYVTAPL 181

Query: 181 SLCSNCLPEVYHFAADGLAQFVFKSKMQMPDVEVSLLDVVIPLTNLGWRQWIGAALFFWG 240
           SLCSN  PEV  F  + +A+F+   K      E +LL  + PL  LG  QWIG A+F WG
Sbjct: 182 SLCSNIAPEVAGFVGNQVAEFIANGKSHTSAPEFNLLSSISPLMKLGSLQWIGGAIFLWG 241

Query: 241 WIHQQRCHLILGSLRVRGGRTEEYRIPHGDWFEIVSSPHYLAEIVIYAGFVVASGGEDFT 300
           WIHQ+RCH ILGSLR    + +EY IP+GDWF +VSSPH+LAEIV+YAG ++ASGG D T
Sbjct: 242 WIHQRRCHAILGSLRENPSQAKEYIIPYGDWFGMVSSPHFLAEIVLYAGLLIASGGTDIT 301

Query: 301 IWLLFGFVVANLAFAAAETHRWYFRKFDNYPRNRFAVIPYIF 339
           IWLLFGFV ANL +AA ETHRWY RKF+NYP NR A+ PY++
Sbjct: 302 IWLLFGFVAANLTYAAGETHRWYLRKFENYPANRHAIFPYVY 343

BLAST of Lag0001513 vs. TAIR 10
Match: AT2G16530.2 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 406.4 bits (1043), Expect = 2.3e-113
Identity = 201/342 (58.77%), Postives = 244/342 (71.35%), Query Frame = 0

Query: 1   MELGIVWLLRAA----WIAAILPILVAFLPFSRLNWFREIVLGFAKRGKILQSSSQKFTV 60
           +EL IVWL+R A    WI +ILP+++A +P S+LN FRE+VL FA RGKIL  SSQ FT+
Sbjct: 2   VELEIVWLVRGAWITVWIVSILPLVIASIPTSKLNSFRELVLSFAGRGKILHPSSQ-FTI 61

Query: 61  PQKFFCHFYVFAVIWTTLLLGTSWIYAYVSTPEIPEPYNFPAITSQLTGGSSLFSWQKSH 120
           PQK F HFYV  V+WTTLLL  +W+YA    P   E +    I S+L GGS +FS  KS+
Sbjct: 62  PQKCFAHFYVIGVVWTTLLLAATWMYACKMAPLSSEEFQLSDIASRLAGGSDVFSVHKSN 121

Query: 121 SSRREQTFLVWKAVFLLLLMEVQVLRRLYETIFVFNYSPSARMHIFGYLTGFFFYTAAPL 180
            +  E  F VW+AVFLLLLME+ VLRRL E+ +VF YSPSARMHI GY  G FFY  APL
Sbjct: 122 MTPVEHRFKVWRAVFLLLLMEIHVLRRLIESFYVFKYSPSARMHILGYFAGLFFYVTAPL 181

Query: 181 SLCSNCLPEVYHFAADGLAQFVFKSKMQMPDVEVSLLDVVIPLTNLGWRQWIGAALFFWG 240
           SLCSN  PEV  F  + +A+F+   K      E +LL  + PL  LG  QWIG A+F WG
Sbjct: 182 SLCSNIAPEVAGFVGNQVAEFIANGKSHTSAPEFNLLSSISPLMKLGSLQWIGGAIFLWG 241

Query: 241 WIHQQRCHLILGSLRVRGGRTEEYRIPHGDWFEIVSSPHYLAEIVIYAGFVVASGGEDFT 300
           WIHQ+RCH ILGSLR    + +EY IP+GDWF +VSSPH+LAEIV+YAG ++ASGG D T
Sbjct: 242 WIHQRRCHAILGSLRENPSQAKEYIIPYGDWFGMVSSPHFLAEIVLYAGLLIASGGTDIT 301

Query: 301 IWLLFGFVVANLAFAAAETHRWYFRKFDNYPRNRFAVIPYIF 339
           IWLLFGFV ANL +AA ETHRWY RKF+NYP NR A+ PY++
Sbjct: 302 IWLLFGFVAANLTYAAGETHRWYLRKFENYPANRHAIFPYVY 342

BLAST of Lag0001513 vs. TAIR 10
Match: AT1G72590.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 368.6 bits (945), Expect = 5.3e-102
Identity = 187/342 (54.68%), Postives = 237/342 (69.30%), Query Frame = 0

Query: 1   MELGIVWLLRAA----WIAAILPILVAFLPFSRLNWFREIVLGFAKRGKILQSSSQKFTV 60
           ME+ IVWL++AA    WI +ILP+++A +P S+LN FRE+VL FA RGKIL  SSQKFTV
Sbjct: 1   MEVEIVWLVKAAWITVWIVSILPLVIASIPSSKLNSFRELVLSFAGRGKILHPSSQKFTV 60

Query: 61  PQKFFCHFYVFAVIWTTLLLGTSWIYAYVSTPEIPEPYNFPAITSQLTGGSSLFSWQKSH 120
           PQKFF HFYV  V+WTTLLL  +W+YA                  ++ GGS +FS+  +H
Sbjct: 61  PQKFFGHFYVVGVVWTTLLLAATWMYA-----------------CKMAGGSHVFSFHMTH 120

Query: 121 SSRREQTFLVWKAVFLLLLMEVQVLRRLYETIFVFNYSPSARMHIFGYLTGFFFYTAAPL 180
              R   F V +AVFLLLLME+ VLRR+ E+ +VF YS SARMHI  Y+   F+Y AAPL
Sbjct: 121 VEHR---FKVGRAVFLLLLMEIHVLRRVIESFYVFKYSTSARMHILAYVGALFYYVAAPL 180

Query: 181 SLCSNCLPEVYHFAADGLAQFVFKSKMQMPDVEVSLLDVVIPLTNLGWRQWIGAALFFWG 240
           SLCSN  PEV  F    +A+F+   K    D   +LL  + PL  LG  QWIG A+F WG
Sbjct: 181 SLCSNIAPEVARFVGSQVAEFIASGKSHSHD--FNLLLSISPLMKLGSLQWIGGAIFLWG 240

Query: 241 WIHQQRCHLILGSLRVRGGRTEEYRIPHGDWFEIVSSPHYLAEIVIYAGFVVASGGEDFT 300
           WIHQ+RCH ILGSLR    + +EY IP+GDWFE+VS PH+LAEIV+Y G +++SGG D +
Sbjct: 241 WIHQRRCHAILGSLREYPSQAKEYIIPYGDWFEMVSCPHFLAEIVLYLGLLISSGGTDIS 300

Query: 301 IWLLFGFVVANLAFAAAETHRWYFRKFDNYPRNRFAVIPYIF 339
           IWLLFGFV ANL +AA ETHRWY +KF+NYP +R A+ P+++
Sbjct: 301 IWLLFGFVAANLTYAAGETHRWYLQKFENYPASRHAIFPHVY 320

BLAST of Lag0001513 vs. TAIR 10
Match: AT3G43840.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 73.2 bits (178), Expect = 4.6e-13
Identity = 35/74 (47.30%), Postives = 46/74 (62.16%), Query Frame = 0

Query: 270 IVSSPHYLAEIVIYAGFVVASGGE-----DFTIWLLFGFVVANLAFAAAETHRWYFRKFD 329
           +VS  + + + V    F+ A   E     +  IWLLFGFV ANL +AA ETHRWY  KF+
Sbjct: 11  VVSETNCVVKFVTLPNFLSAFKYEVKKSANLPIWLLFGFVAANLTYAAGETHRWYLAKFE 70

Query: 330 NYPRNRFAVIPYIF 339
           NYP NR A+ PY++
Sbjct: 71  NYPANRHAIFPYVY 84

BLAST of Lag0001513 vs. TAIR 10
Match: AT5G16010.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 67.4 bits (163), Expect = 2.5e-11
Identity = 38/113 (33.63%), Postives = 62/113 (54.87%), Query Frame = 0

Query: 229 GAALFFWGWIHQQRCHLILGSLRVRGGRTEEYRIPHGDWFEIVSSPHYLAEIVIYAGFVV 288
           G  +F  G +     H++L  LR   G+ +EY+IP G  F+I+  PHYL EI+++  F +
Sbjct: 162 GVVMFVVGIVGNLYHHVLLAKLRKEDGK-KEYKIPKGGLFDIIICPHYLFEILVFWSFFL 221

Query: 289 ASGGEDFTIWLLFGFVVANLAFAAAE---THRWYFRKFDNYPRNRFAVIPYIF 339
            S     TI+  F F +  + +       T  WY  KFD++P++  A+IP++F
Sbjct: 222 ISQ----TIY-SFSFAMGTMLYLIGRSYATRTWYLSKFDDFPKHIKALIPFVF 268

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_004148545.12.4e-17387.28polyprenol reductase 2 [Cucumis sativus] >KGN43186.1 hypothetical protein Csa_02... [more]
XP_038888381.15.6e-17086.98polyprenol reductase 2 isoform X2 [Benincasa hispida][more]
XP_008444886.11.2e-16985.50PREDICTED: polyprenol reductase 2 [Cucumis melo][more]
KAG7022255.11.9e-16583.73Polyprenol reductase 2 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_023530646.13.2e-16584.57polyprenol reductase 2-like isoform X1 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q9SI622.6e-11459.06Polyprenol reductase 2 OS=Arabidopsis thaliana OX=3702 GN=PPRD2 PE=1 SV=2[more]
Q9CAH57.4e-10154.68Polyprenol reductase 1 OS=Arabidopsis thaliana OX=3702 GN=PPRD1 PE=1 SV=1[more]
A2XWN65.5e-9652.33Polyprenol reductase 1 OS=Oryza sativa subsp. indica OX=39946 GN=OsI_17065 PE=3 ... [more]
Q7XUH55.5e-9652.33Polyprenol reductase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0576800 ... [more]
Q7F0Q29.4e-8851.16Polyprenol reductase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os07g0493400 ... [more]
Match NameE-valueIdentityDescription
A0A0A0K5R71.2e-17387.28Polyprenol reductase OS=Cucumis sativus OX=3659 GN=Csa_7G007840 PE=3 SV=1[more]
A0A1S3BBF06.0e-17085.50Polyprenol reductase OS=Cucumis melo OX=3656 GN=LOC103488097 PE=3 SV=1[more]
A0A6J1I5R22.6e-16583.73Polyprenol reductase OS=Cucurbita maxima OX=3661 GN=LOC111469837 PE=3 SV=1[more]
A0A6J1F6C02.2e-16483.43Polyprenol reductase OS=Cucurbita moschata OX=3662 GN=LOC111441221 PE=3 SV=1[more]
A0A6J1C1967.6e-15781.66Polyprenol reductase OS=Momordica charantia OX=3673 GN=LOC111007527 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT2G16530.11.9e-11559.063-oxo-5-alpha-steroid 4-dehydrogenase family protein [more]
AT2G16530.22.3e-11358.773-oxo-5-alpha-steroid 4-dehydrogenase family protein [more]
AT1G72590.15.3e-10254.683-oxo-5-alpha-steroid 4-dehydrogenase family protein [more]
AT3G43840.14.6e-1347.303-oxo-5-alpha-steroid 4-dehydrogenase family protein [more]
AT5G16010.12.5e-1133.633-oxo-5-alpha-steroid 4-dehydrogenase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR0011043-oxo-5-alpha-steroid 4-dehydrogenase, C-terminalPFAMPF02544Steroid_dhcoord: 224..338
e-value: 1.2E-26
score: 93.5
IPR0011043-oxo-5-alpha-steroid 4-dehydrogenase, C-terminalPROSITEPS50244S5A_REDUCTASEcoord: 229..313
score: 20.89065
NoneNo IPR availablePANTHERPTHR14624:SF2POLYPRENOL REDUCTASE 2-LIKEcoord: 1..338
IPR039698Polyprenol reductasePANTHERPTHR14624DFG10 PROTEINcoord: 1..338

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0001513.1Lag0001513.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0019408 dolichol biosynthetic process
biological_process GO:0006488 dolichol-linked oligosaccharide biosynthetic process
biological_process GO:0016095 polyprenol catabolic process
biological_process GO:0006629 lipid metabolic process
cellular_component GO:0005789 endoplasmic reticulum membrane
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0003865 3-oxo-5-alpha-steroid 4-dehydrogenase activity
molecular_function GO:0102389 polyprenol reductase activity
molecular_function GO:0016627 oxidoreductase activity, acting on the CH-CH group of donors