Lag0001460 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0001460
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionProtein of unknown function (DUF803)
Locationchr4: 31594082 .. 31597041 (-)
RNA-Seq ExpressionLag0001460
SyntenyLag0001460
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTGATTTCGGAGAACACGAAGGGTCTGATTCTGGCTGTGGCGTCCAGTGCCTTTATTGGGTCGAGTTTTATTTTGAAGAAGAAAGGACTTAAGCGTGCCGGAGCCACAGGGGCGAGAGCAGGTCTGTGATTGCGATTCGTGAATGGTTTTTTTTTATTTGTTGGTTCTGGTTTTGGTTTTGCCTCCTTGTTGAATTGTTCTACCGATTATGCAATGATTGATTTCTGTGCTGTTGAATGTATTTGTTGGTTGGAATTTGGAGGGCAATCTGATCCATTTTGGATTTGTTCTTTCTGTATTTTGCTTTATATCCAAGGAAAGGGAAGTTTCTTTCAGAACTTTTGGGTTCTTTGACGTGGGAATGCTGTAGTAGTTTGTTCCGTGTGGACTTTTCTTCTTGATTTGTTTATAATTGGTGGCATTTATTCATGAATGGATCATAGCAGACAGGGCAAATTGCTTGTAAATGGATGGAAGCTTTAAGGGTTGGGATTGGATTCTTCAATAGATCATGGATTCAGAACACCATGAGCCACCTTTTGGATGTCAGATGGAAGCTTTAAGGGGGTTGGGATTTGAGGTTCTTTGCAATGTTAAGCTGTAGATAGAGTTAATATCATCTTAATGAACTGACAACATTAAAGATCTTCAATGAATGAATTTATTTCTTCATCTTCTTTGTTATTGCTTCTAATGGACCTTTACTTCAAGAGTTTTCTAGGCAATATTCATGTTCTCTTACTGGTTCATGCTTATAATCTCTTTCTTAAAACAGGTGTTGGCGGTTATACATACCTGTTAGAGCCACTTTGGTGGGCAGGCATGGTTACAAGTATGTATAATAATACTATTTAGGCAACCAACCCCCTTCCCTCCAAGCCCCCTATTATGTCTGATTGTTATTATCTAAGAGATGAAGTTGATCTTGATCTTGAGTTTTCTTCTTGTTCAATAGTGATAATTGGAGAGATTGCGAACTTTGTAGCATATATTTATGCCCCAGCAGTTTTAGTCACACCCCTTGGTGCGCTGAGCATAATTATCAGGTAAGTCCATTTCTTTTGAGATTCCTGTGCTATTTGTGTCTAAAATATTGCTCATGAGTTCATGGGGGATCTCACATTTTCTCTGGATTTGTGCAGTGCTGTTTTAGCCCACTTCTTATTGAAGGAGAGGCTACAGAAAATGGGTATTGTAGGGTGTTTATCCTGTATTGTAGGATCAGTTATAATAGTTGTCCATGCACCTCAGGAACATACTCCTGATTCTGTTGAAGAAATTTGGGATTTAGCAACCCAACCAGGTCAGAGTTTATTCTTATGAATATCATTTAATGAAGCCTCATCAAATTTAGTACTTAATTTCCTTTTTCTTAATCTTTGAACAGCTTTTCTAGTCTACATAGCTGCCACAGCTTCGTTAGTATTGGCTCTTATGTTGTATTTTGAACCTCGATATGGACATGTAAACATACTAGTCTACTTGGGAATCTGTTCTTTAATGGGTTCATTGACGGTAATTATACCATTTCATTTCTGCTAGATCAGTACGTATGTTTGCTGATGTGTCTATCTTACAATCATGATTTGAATAGCTATTTTCTAAACTTTTGTGATTTATTCAGGTTATGAGTATTAAAGCTATTGGAATAGCGATAAAGCTTACACTTGAAGGTGTAAGCCAGGTAGCCTATCCTCAGACTTGGCTTTTTGTCACAGTTGCAGTAGTCTGCGTCATCACACAACTGAATTACTTGAATAAGGTTGGTGTGATTTAATTGTGGAAAAAGTTGTTTCCTACTCCTTTCTTAACCAAATGCTTGAACAGGATGGTGTATTTATGTTGACTGATATGTTCTCTTTTATTTCAGGCATTGGATACATTCAACGCAGCACTTGTATCTCCAGTCTATTATGCCATGTTCACGACATTGACTATTATAGCTAGTGCCATCATGTTTAAGGTAAATTACTTGACCATATGGTTCCTCGGATCTTGGATATAATAATATCCATACAAGTGTAAAAAAAAATGTAACTTTTGAAAAATAGAAAATAAATCGATGAATGTGAATCGGTTGAGTTGGTCTATGCTGCAGGACTGGTCAGACCAGGATGCGAGCACCATAGTCTCTGAATTATGTGGATTCGTCACTGTCCTTTCTGGAACTATTATTCTTCACTCAACCAGAGAACAGCAAACAGTCTCCTCACAAGGTATGATATCTAAACATCTCTTTTGCTTTTCCCTGTTGTCCATGAATTGAGAAATCTTGGTATGGTTTGAAGCTAATCAATTATTGTGTGATCTGGTGAACTCGAAATGATTGTGTATGCGTGCACGTATAAACTACATAGCATCAAATTCAAGAACGGTGCTTAAGACATCTGTTTAGTGTATCTGAATCTGTGATCTGTTCTTAGAAACACAGCTGAACTATCAACCCTCTTTTATATGGTACCATTTTGCAAAATTTGGTCTTAATACACGGGGCTTAGAAGGAATAAGTTCCAAGTCATGGTGCCACCTATCTAGGATTTAATATCCAACAAGTTATCTTGGCAACCAAATGTAGGAAGATCAAACGGCTGTCTCATTATAATAGTCAATGTGGACACAAACTAGCCGATAAAAAAAAAAAAAAAAAAACTATGGTCTTATACACACCTCTCCCACGCTCCCTGCCCTAACAAGAAGGGGTTAAATAATAGTAACAGAACTTTGGTTTCTGCTGAGTGAATATGAATTTCATTTACCTATTTCTTTAATAATGTGGCAGTGATTAATGTTTCATAGACCTAATTTAACTTGTAAATGGACAATAGAACTTGTAGTGTACACATAAGTTAATACTTCTCTTTGCAGGATCTGTGGCATGGTATATAACTGGGGATTCAATGAAAGGTATTGAAGAACATTTGATCACCATAAGCAATTCACATTACACTGAAGAATGA

mRNA sequence

ATGGTGATTTCGGAGAACACGAAGGGTCTGATTCTGGCTGTGGCGTCCAGTGCCTTTATTGGGTCGAGTTTTATTTTGAAGAAGAAAGGACTTAAGCGTGCCGGAGCCACAGGGGCGAGAGCAGGTGTTGGCGGTTATACATACCTGTTAGAGCCACTTTGGTGGGCAGGCATGGTTACAATGATAATTGGAGAGATTGCGAACTTTGTAGCATATATTTATGCCCCAGCAGTTTTAGTCACACCCCTTGGTGCGCTGAGCATAATTATCAGTGCTGTTTTAGCCCACTTCTTATTGAAGGAGAGGCTACAGAAAATGGGTATTGTAGGGTGTTTATCCTGTATTGTAGGATCAGTTATAATAGTTGTCCATGCACCTCAGGAACATACTCCTGATTCTGTTGAAGAAATTTGGGATTTAGCAACCCAACCAGCTTTTCTAGTCTACATAGCTGCCACAGCTTCGTTAGTATTGGCTCTTATGTTGTATTTTGAACCTCGATATGGACATGTAAACATACTAGTCTACTTGGGAATCTGTTCTTTAATGGGTTCATTGACGGTTATGAGTATTAAAGCTATTGGAATAGCGATAAAGCTTACACTTGAAGGTGTAAGCCAGGTAGCCTATCCTCAGACTTGGCTTTTTGTCACAGTTGCAGTAGTCTGCGTCATCACACAACTGAATTACTTGAATAAGGCATTGGATACATTCAACGCAGCACTTGTATCTCCAGTCTATTATGCCATGTTCACGACATTGACTATTATAGCTAGTGCCATCATGTTTAAGGACTGGTCAGACCAGGATGCGAGCACCATAGTCTCTGAATTATGTGGATTCGTCACTGTCCTTTCTGGAACTATTATTCTTCACTCAACCAGAGAACAGCAAACAGTCTCCTCACAAGGATCTGTGGCATGGTATATAACTGGGGATTCAATGAAAGGTATTGAAGAACATTTGATCACCATAAGCAATTCACATTACACTGAAGAATGA

Coding sequence (CDS)

ATGGTGATTTCGGAGAACACGAAGGGTCTGATTCTGGCTGTGGCGTCCAGTGCCTTTATTGGGTCGAGTTTTATTTTGAAGAAGAAAGGACTTAAGCGTGCCGGAGCCACAGGGGCGAGAGCAGGTGTTGGCGGTTATACATACCTGTTAGAGCCACTTTGGTGGGCAGGCATGGTTACAATGATAATTGGAGAGATTGCGAACTTTGTAGCATATATTTATGCCCCAGCAGTTTTAGTCACACCCCTTGGTGCGCTGAGCATAATTATCAGTGCTGTTTTAGCCCACTTCTTATTGAAGGAGAGGCTACAGAAAATGGGTATTGTAGGGTGTTTATCCTGTATTGTAGGATCAGTTATAATAGTTGTCCATGCACCTCAGGAACATACTCCTGATTCTGTTGAAGAAATTTGGGATTTAGCAACCCAACCAGCTTTTCTAGTCTACATAGCTGCCACAGCTTCGTTAGTATTGGCTCTTATGTTGTATTTTGAACCTCGATATGGACATGTAAACATACTAGTCTACTTGGGAATCTGTTCTTTAATGGGTTCATTGACGGTTATGAGTATTAAAGCTATTGGAATAGCGATAAAGCTTACACTTGAAGGTGTAAGCCAGGTAGCCTATCCTCAGACTTGGCTTTTTGTCACAGTTGCAGTAGTCTGCGTCATCACACAACTGAATTACTTGAATAAGGCATTGGATACATTCAACGCAGCACTTGTATCTCCAGTCTATTATGCCATGTTCACGACATTGACTATTATAGCTAGTGCCATCATGTTTAAGGACTGGTCAGACCAGGATGCGAGCACCATAGTCTCTGAATTATGTGGATTCGTCACTGTCCTTTCTGGAACTATTATTCTTCACTCAACCAGAGAACAGCAAACAGTCTCCTCACAAGGATCTGTGGCATGGTATATAACTGGGGATTCAATGAAAGGTATTGAAGAACATTTGATCACCATAAGCAATTCACATTACACTGAAGAATGA

Protein sequence

MVISENTKGLILAVASSAFIGSSFILKKKGLKRAGATGARAGVGGYTYLLEPLWWAGMVTMIIGEIANFVAYIYAPAVLVTPLGALSIIISAVLAHFLLKERLQKMGIVGCLSCIVGSVIIVVHAPQEHTPDSVEEIWDLATQPAFLVYIAATASLVLALMLYFEPRYGHVNILVYLGICSLMGSLTVMSIKAIGIAIKLTLEGVSQVAYPQTWLFVTVAVVCVITQLNYLNKALDTFNAALVSPVYYAMFTTLTIIASAIMFKDWSDQDASTIVSELCGFVTVLSGTIILHSTREQQTVSSQGSVAWYITGDSMKGIEEHLITISNSHYTEE
Homology
BLAST of Lag0001460 vs. NCBI nr
Match: XP_008447976.1 (PREDICTED: probable magnesium transporter NIPA6 isoform X1 [Cucumis melo] >XP_008447977.1 PREDICTED: probable magnesium transporter NIPA6 isoform X1 [Cucumis melo])

HSP 1 Score: 609.8 bits (1571), Expect = 1.4e-170
Identity = 318/333 (95.50%), Postives = 328/333 (98.50%), Query Frame = 0

Query: 1   MVISENTKGLILAVASSAFIGSSFILKKKGLKRAGATGARAGVGGYTYLLEPLWWAGMVT 60
           M ISENTKGLILA+ASSAFIGSSFILKKKGLKRAGATGARAGVGGYTYLLEPLWWAGM+T
Sbjct: 1   MTISENTKGLILAMASSAFIGSSFILKKKGLKRAGATGARAGVGGYTYLLEPLWWAGMIT 60

Query: 61  MIIGEIANFVAYIYAPAVLVTPLGALSIIISAVLAHFLLKERLQKMGIVGCLSCIVGSVI 120
           MIIGEIANFVAYIYAPAVLVTPLGALSII+SAVLAHFLLKERLQKMG+VGCLSCIVGSVI
Sbjct: 61  MIIGEIANFVAYIYAPAVLVTPLGALSIIVSAVLAHFLLKERLQKMGVVGCLSCIVGSVI 120

Query: 121 IVVHAPQEHTPDSVEEIWDLATQPAFLVYIAATASLVLALMLYFEPRYGHVNILVYLGIC 180
           IV+HAPQEHTPDSVEEIWDLATQPAFLVYIAA ASLVLALMLYFEPRYGHVNILVYLGIC
Sbjct: 121 IVIHAPQEHTPDSVEEIWDLATQPAFLVYIAAIASLVLALMLYFEPRYGHVNILVYLGIC 180

Query: 181 SLMGSLTVMSIKAIGIAIKLTLEGVSQVAYPQTWLFVTVAVVCVITQLNYLNKALDTFNA 240
           SLMGSLTVMSIKAIGIAI+LTLEG+SQVAYPQTWLFVTVAVVCVITQLNYLNKALDTFNA
Sbjct: 181 SLMGSLTVMSIKAIGIAIRLTLEGISQVAYPQTWLFVTVAVVCVITQLNYLNKALDTFNA 240

Query: 241 ALVSPVYYAMFTTLTIIASAIMFKDWSDQDASTIVSELCGFVTVLSGTIILHSTREQQTV 300
           ALVSPVYYAMFTTLTIIASAIMFKDWS Q+ASTIVSELCGF+TVLSGTIILHSTREQQ V
Sbjct: 241 ALVSPVYYAMFTTLTIIASAIMFKDWSGQNASTIVSELCGFITVLSGTIILHSTREQQPV 300

Query: 301 SSQGSVAWYITGDSMKGIEEHLITISNSHYTEE 334
           SSQGSVAWYI+GDSMK IEEHLITISNSHYTEE
Sbjct: 301 SSQGSVAWYISGDSMKSIEEHLITISNSHYTEE 333

BLAST of Lag0001460 vs. NCBI nr
Match: XP_022952466.1 (probable magnesium transporter NIPA6 isoform X1 [Cucurbita moschata] >KAG6572100.1 putative magnesium transporter NIPA6, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 607.4 bits (1565), Expect = 7.2e-170
Identity = 316/332 (95.18%), Postives = 327/332 (98.49%), Query Frame = 0

Query: 1   MVISENTKGLILAVASSAFIGSSFILKKKGLKRAGATGARAGVGGYTYLLEPLWWAGMVT 60
           M ISENTKGL+LA+ASSAFIGSSFILKKKGLKRAGATGARAGVGGYTYLLEPLWWAGMVT
Sbjct: 1   MTISENTKGLVLAMASSAFIGSSFILKKKGLKRAGATGARAGVGGYTYLLEPLWWAGMVT 60

Query: 61  MIIGEIANFVAYIYAPAVLVTPLGALSIIISAVLAHFLLKERLQKMGIVGCLSCIVGSVI 120
           MIIGEIANF+AYIYAPAVLVTPLGALSIIISAVLAHFLLKERLQKMG++GCLSCIVGSVI
Sbjct: 61  MIIGEIANFIAYIYAPAVLVTPLGALSIIISAVLAHFLLKERLQKMGVLGCLSCIVGSVI 120

Query: 121 IVVHAPQEHTPDSVEEIWDLATQPAFLVYIAATASLVLALMLYFEPRYGHVNILVYLGIC 180
           IV+HAPQEHTPDSV+EIWDLATQPAFLVYIA  ASLVLALMLYFEPRYGHVNILVYLGIC
Sbjct: 121 IVIHAPQEHTPDSVQEIWDLATQPAFLVYIATIASLVLALMLYFEPRYGHVNILVYLGIC 180

Query: 181 SLMGSLTVMSIKAIGIAIKLTLEGVSQVAYPQTWLFVTVAVVCVITQLNYLNKALDTFNA 240
           SLMGSLTVMSIKAIGIAI+LTLEGVSQVAYPQTWLFVTVAVVCVITQLNYLNKALDTFNA
Sbjct: 181 SLMGSLTVMSIKAIGIAIRLTLEGVSQVAYPQTWLFVTVAVVCVITQLNYLNKALDTFNA 240

Query: 241 ALVSPVYYAMFTTLTIIASAIMFKDWSDQDASTIVSELCGFVTVLSGTIILHSTREQQTV 300
           ALVSPVYYAMFTTLTIIASAIMFKDWS Q+A+TIVSELCGF+TVLSGTIILHSTREQQ V
Sbjct: 241 ALVSPVYYAMFTTLTIIASAIMFKDWSGQNANTIVSELCGFITVLSGTIILHSTREQQPV 300

Query: 301 SSQGSVAWYITGDSMKGIEEHLITISNSHYTE 333
           SSQGSVAWYITGDSMKGIEEHLITISNSHYTE
Sbjct: 301 SSQGSVAWYITGDSMKGIEEHLITISNSHYTE 332

BLAST of Lag0001460 vs. NCBI nr
Match: XP_004144837.3 (probable magnesium transporter NIPA6 isoform X1 [Cucumis sativus] >KGN43231.1 hypothetical protein Csa_020367 [Cucumis sativus])

HSP 1 Score: 607.1 bits (1564), Expect = 9.3e-170
Identity = 317/333 (95.20%), Postives = 327/333 (98.20%), Query Frame = 0

Query: 1   MVISENTKGLILAVASSAFIGSSFILKKKGLKRAGATGARAGVGGYTYLLEPLWWAGMVT 60
           M ISEN+KGLILA+ASSAFIGSSFILKKKGLKRAGATGARAGVGGYTYLLEPLWWAGM+T
Sbjct: 1   MTISENSKGLILAMASSAFIGSSFILKKKGLKRAGATGARAGVGGYTYLLEPLWWAGMIT 60

Query: 61  MIIGEIANFVAYIYAPAVLVTPLGALSIIISAVLAHFLLKERLQKMGIVGCLSCIVGSVI 120
           MIIGEIANFVAYIYAPAVLVTPLGALSII+SAVLAHFLLKERLQKMG+VGCLSCIVGSVI
Sbjct: 61  MIIGEIANFVAYIYAPAVLVTPLGALSIIVSAVLAHFLLKERLQKMGVVGCLSCIVGSVI 120

Query: 121 IVVHAPQEHTPDSVEEIWDLATQPAFLVYIAATASLVLALMLYFEPRYGHVNILVYLGIC 180
           IV+HAPQEHTPDSVEEIWDLATQPAFLVYIAA ASLVLALMLYFEPRYGHVNILVYLGIC
Sbjct: 121 IVIHAPQEHTPDSVEEIWDLATQPAFLVYIAAIASLVLALMLYFEPRYGHVNILVYLGIC 180

Query: 181 SLMGSLTVMSIKAIGIAIKLTLEGVSQVAYPQTWLFVTVAVVCVITQLNYLNKALDTFNA 240
           SLMGSLTVMSIKAIGIAI+LTLEG+SQVAYPQTWLFVTVAVVCVITQLNYLNKALDTFNA
Sbjct: 181 SLMGSLTVMSIKAIGIAIRLTLEGISQVAYPQTWLFVTVAVVCVITQLNYLNKALDTFNA 240

Query: 241 ALVSPVYYAMFTTLTIIASAIMFKDWSDQDASTIVSELCGFVTVLSGTIILHSTREQQTV 300
           ALVSPVYYAMFTTLTIIASAIMFKDWS Q+ASTIVSELCGFVTVLSGTIILHSTREQQ V
Sbjct: 241 ALVSPVYYAMFTTLTIIASAIMFKDWSGQNASTIVSELCGFVTVLSGTIILHSTREQQPV 300

Query: 301 SSQGSVAWYITGDSMKGIEEHLITISNSHYTEE 334
           SSQGSVAWYI+GDSMK  EEHLITISNSHYTEE
Sbjct: 301 SSQGSVAWYISGDSMKSFEEHLITISNSHYTEE 333

BLAST of Lag0001460 vs. NCBI nr
Match: KAG7011752.1 (putative magnesium transporter NIPA6, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 607.1 bits (1564), Expect = 9.3e-170
Identity = 315/332 (94.88%), Postives = 327/332 (98.49%), Query Frame = 0

Query: 1   MVISENTKGLILAVASSAFIGSSFILKKKGLKRAGATGARAGVGGYTYLLEPLWWAGMVT 60
           M ISENTKGL+LA+ASSAFIGSSFILKKKGLKRAGATGARAGVGGYTYLLEPLWWAGMVT
Sbjct: 1   MTISENTKGLVLAMASSAFIGSSFILKKKGLKRAGATGARAGVGGYTYLLEPLWWAGMVT 60

Query: 61  MIIGEIANFVAYIYAPAVLVTPLGALSIIISAVLAHFLLKERLQKMGIVGCLSCIVGSVI 120
           MIIGEIANF+AYIYAPAVLVTPLGALSIIISAVLAHFLLKERLQKMG++GCLSCIVGSVI
Sbjct: 61  MIIGEIANFIAYIYAPAVLVTPLGALSIIISAVLAHFLLKERLQKMGVLGCLSCIVGSVI 120

Query: 121 IVVHAPQEHTPDSVEEIWDLATQPAFLVYIAATASLVLALMLYFEPRYGHVNILVYLGIC 180
           IV+HAPQEHTPDSV+EIWDLATQPAFLVYIA  ASLVLALMLYFEPRYGHVNIL+YLGIC
Sbjct: 121 IVIHAPQEHTPDSVQEIWDLATQPAFLVYIATIASLVLALMLYFEPRYGHVNILIYLGIC 180

Query: 181 SLMGSLTVMSIKAIGIAIKLTLEGVSQVAYPQTWLFVTVAVVCVITQLNYLNKALDTFNA 240
           SLMGSLTVMSIKAIGIAI+LTLEGVSQVAYPQTWLFVTVAVVCVITQLNYLNKALDTFNA
Sbjct: 181 SLMGSLTVMSIKAIGIAIRLTLEGVSQVAYPQTWLFVTVAVVCVITQLNYLNKALDTFNA 240

Query: 241 ALVSPVYYAMFTTLTIIASAIMFKDWSDQDASTIVSELCGFVTVLSGTIILHSTREQQTV 300
           ALVSPVYYAMFTTLTIIASAIMFKDWS Q+A+TIVSELCGF+TVLSGTIILHSTREQQ V
Sbjct: 241 ALVSPVYYAMFTTLTIIASAIMFKDWSGQNANTIVSELCGFITVLSGTIILHSTREQQPV 300

Query: 301 SSQGSVAWYITGDSMKGIEEHLITISNSHYTE 333
           SSQGSVAWYITGDSMKGIEEHLITISNSHYTE
Sbjct: 301 SSQGSVAWYITGDSMKGIEEHLITISNSHYTE 332

BLAST of Lag0001460 vs. NCBI nr
Match: XP_038886953.1 (probable magnesium transporter NIPA6 isoform X1 [Benincasa hispida])

HSP 1 Score: 606.7 bits (1563), Expect = 1.2e-169
Identity = 316/333 (94.89%), Postives = 328/333 (98.50%), Query Frame = 0

Query: 1   MVISENTKGLILAVASSAFIGSSFILKKKGLKRAGATGARAGVGGYTYLLEPLWWAGMVT 60
           M ISENTKGL+LA+ASSAFIGSSFILKKKGLKRAGATGARAGVGGYTYLLEPLWWAGM+T
Sbjct: 1   MTISENTKGLVLAMASSAFIGSSFILKKKGLKRAGATGARAGVGGYTYLLEPLWWAGMIT 60

Query: 61  MIIGEIANFVAYIYAPAVLVTPLGALSIIISAVLAHFLLKERLQKMGIVGCLSCIVGSVI 120
           MIIGEIANFVAYIYAPAVLVTPLGALSII+SAVLAHFLL+ERLQKMG+VGCLSCIVGSVI
Sbjct: 61  MIIGEIANFVAYIYAPAVLVTPLGALSIIVSAVLAHFLLRERLQKMGVVGCLSCIVGSVI 120

Query: 121 IVVHAPQEHTPDSVEEIWDLATQPAFLVYIAATASLVLALMLYFEPRYGHVNILVYLGIC 180
           IV+HAPQE TPDSVEEIWDLATQPAFLVYIAA ASLVLALMLYFEPRYGHVNILVYLGIC
Sbjct: 121 IVIHAPQERTPDSVEEIWDLATQPAFLVYIAAIASLVLALMLYFEPRYGHVNILVYLGIC 180

Query: 181 SLMGSLTVMSIKAIGIAIKLTLEGVSQVAYPQTWLFVTVAVVCVITQLNYLNKALDTFNA 240
           SLMGSLTVMSIKAIGIAI+LTL+GVSQVAYPQTWLFVTVAVVCVITQLNYLNKALDTFNA
Sbjct: 181 SLMGSLTVMSIKAIGIAIRLTLDGVSQVAYPQTWLFVTVAVVCVITQLNYLNKALDTFNA 240

Query: 241 ALVSPVYYAMFTTLTIIASAIMFKDWSDQDASTIVSELCGFVTVLSGTIILHSTREQQTV 300
           ALVSPVYYAMFTTLTIIASAIMFKDWS Q+ASTIVSELCGF+TVLSGTIILHSTREQQ V
Sbjct: 241 ALVSPVYYAMFTTLTIIASAIMFKDWSGQNASTIVSELCGFITVLSGTIILHSTREQQPV 300

Query: 301 SSQGSVAWYITGDSMKGIEEHLITISNSHYTEE 334
           SSQGSVAWYI+GDSMKGIEEHLITISNSHYTEE
Sbjct: 301 SSQGSVAWYISGDSMKGIEEHLITISNSHYTEE 333

BLAST of Lag0001460 vs. ExPASy Swiss-Prot
Match: Q8GWX2 (Probable magnesium transporter NIPA6 OS=Arabidopsis thaliana OX=3702 GN=At2g21120 PE=2 SV=1)

HSP 1 Score: 490.7 bits (1262), Expect = 1.3e-137
Identity = 253/328 (77.13%), Postives = 291/328 (88.72%), Query Frame = 0

Query: 4   SENTKGLILAVASSAFIGSSFILKKKGLKRAGATGARAGVGGYTYLLEPLWWAGMVTMII 63
           ++N KGLILAVASS FIGSSFILKKKGLKRAGA G RAG GGYTYLLEPLWWAGMVTMI+
Sbjct: 3   TDNGKGLILAVASSVFIGSSFILKKKGLKRAGAIGTRAGYGGYTYLLEPLWWAGMVTMIV 62

Query: 64  GEIANFVAYIYAPAVLVTPLGALSIIISAVLAHFLLKERLQKMGIVGCLSCIVGSVIIVV 123
           GE ANFVAYIYAPAVLVTPLGALSIIISAVLAHFLLKE+L+KMG++GC+SCIVGSV+IV+
Sbjct: 63  GEAANFVAYIYAPAVLVTPLGALSIIISAVLAHFLLKEKLKKMGVLGCVSCIVGSVVIVI 122

Query: 124 HAPQEHTPDSVEEIWDLATQPAFLVYIAATASLVLALMLYFEPRYGHVNILVYLGICSLM 183
           HAP+E TP+SVEEIW+LATQPAFL+Y+A T S+VLAL+L+FEP  G  NILVY+GICSLM
Sbjct: 123 HAPKEQTPNSVEEIWNLATQPAFLIYVAITMSIVLALILHFEPLCGQTNILVYIGICSLM 182

Query: 184 GSLTVMSIKAIGIAIKLTLEGVSQVAYPQTWLFVTVAVVCVITQLNYLNKALDTFNAALV 243
           G+LTVMSIKAIGIAIKLT+EGVSQ+ YPQTWLFV VAV CV+TQL YLNKALDTFNAA+V
Sbjct: 183 GALTVMSIKAIGIAIKLTMEGVSQIGYPQTWLFVMVAVTCVVTQLIYLNKALDTFNAAIV 242

Query: 244 SPVYYAMFTTLTIIASAIMFKDWSDQDASTIVSELCGFVTVLSGTIILHSTR-EQQTVSS 303
           SPVYY MFTTLTI+ASAIMFKDWS QDA+++ SELCGF+TVL+GT+ILH TR E+Q  +S
Sbjct: 243 SPVYYVMFTTLTIVASAIMFKDWSGQDAASVASELCGFITVLTGTMILHGTREEEQQQAS 302

Query: 304 QGSVAWYITGDSMKGIEEHLITISNSHY 331
              V WY +  SM   EEHL+++ +  Y
Sbjct: 303 SEHVRWYDSRKSMN--EEHLVSLYSPEY 328

BLAST of Lag0001460 vs. ExPASy Swiss-Prot
Match: Q8GYS1 (Probable magnesium transporter NIPA7 OS=Arabidopsis thaliana OX=3702 GN=At4g38730 PE=2 SV=1)

HSP 1 Score: 453.8 bits (1166), Expect = 1.7e-126
Identity = 230/329 (69.91%), Postives = 276/329 (83.89%), Query Frame = 0

Query: 2   VISENTKGLILAVASSAFIGSSFILKKKGLKRAGATGARAGVGGYTYLLEPLWWAGMVTM 61
           ++S+N  GL+LAV+SS FIGSSFILKKKGLKRA A G RAG GGYTYLLEPLWW G+VTM
Sbjct: 1   MVSDNEMGLVLAVSSSVFIGSSFILKKKGLKRAAANGTRAGFGGYTYLLEPLWWVGLVTM 60

Query: 62  IIGEIANFVAYIYAPAVLVTPLGALSIIISAVLAHFLLKERLQKMGIVGCLSCIVGSVII 121
             GEIANFVAY+YAPAVLVTPLGALSIIISAVLAHFLL E+L+KMG+ GC+ CIVGSV+I
Sbjct: 61  TFGEIANFVAYVYAPAVLVTPLGALSIIISAVLAHFLLDEKLRKMGVWGCVCCIVGSVMI 120

Query: 122 VVHAPQEHTPDSVEEIWDLATQPAFLVYIAATASLVLALMLYFEPRYGHVNILVYLGICS 181
           V+HAPQE TP+SVEEIW LA QPAFL+Y+A + S+VLAL+LY EP  G  NILVY+GICS
Sbjct: 121 VIHAPQEQTPNSVEEIWKLAMQPAFLIYVAISMSIVLALILYCEPLCGQTNILVYIGICS 180

Query: 182 LMGSLTVMSIKAIGIAIKLTLEGVSQVAYPQTWLFVTVAVVCVITQLNYLNKALDTFNAA 241
           LMGSLTVMSIKA+GIAIKLT EG++Q+ YP+TW F  VA +CV+ Q+ YLNKALDTFNAA
Sbjct: 181 LMGSLTVMSIKAVGIAIKLTFEGINQIWYPETWFFAMVAAICVVMQMIYLNKALDTFNAA 240

Query: 242 LVSPVYYAMFTTLTIIASAIMFKDWSDQDASTIVSELCGFVTVLSGTIILHSTREQQTVS 301
           +VSP+YY MFTTLTI+ASAIMFKDW+ Q+  +I SE+CGF+TVL+GT+ILHSTRE++  S
Sbjct: 241 IVSPIYYVMFTTLTIVASAIMFKDWNGQNTDSIASEICGFITVLTGTVILHSTREEEQAS 300

Query: 302 SQGSVAWYITGDSMKGIEEHLITISNSHY 331
            +  + W  +G S    EEHL ++ +  Y
Sbjct: 301 PR-RMRWQDSGKSFD--EEHLTSLYSPEY 326

BLAST of Lag0001460 vs. ExPASy Swiss-Prot
Match: Q9LNK7 (Probable magnesium transporter NIPA3 OS=Arabidopsis thaliana OX=3702 GN=At1g34470 PE=2 SV=1)

HSP 1 Score: 401.7 bits (1031), Expect = 7.8e-111
Identity = 193/299 (64.55%), Postives = 249/299 (83.28%), Query Frame = 0

Query: 4   SENTKGLILAVASSAFIGSSFILKKKGLKRAGATGARAGVGGYTYLLEPLWWAGMVTMII 63
           S+N KGL+LA++SS FIG+SFI+KKKGLKRAGA+G RAG GGY+YLLEPLWW GM+TMI+
Sbjct: 17  SDNIKGLVLALSSSLFIGASFIVKKKGLKRAGASGLRAGSGGYSYLLEPLWWVGMITMIV 76

Query: 64  GEIANFVAYIYAPAVLVTPLGALSIIISAVLAHFLLKERLQKMGIVGCLSCIVGSVIIVV 123
           GEIANF AY +APA+LVTPLGALSIIISA LAH +L E+L   G++GC+ C+VGS+ IV+
Sbjct: 77  GEIANFAAYAFAPAILVTPLGALSIIISAALAHVILHEKLHTFGLLGCVLCVVGSITIVL 136

Query: 124 HAPQEHTPDSVEEIWDLATQPAFLVYIAATASLVLALMLYFEPRYGHVNILVYLGICSLM 183
           HAPQE   DSV ++W+LAT+PAFL+Y AA     + L++ F P+YG  +++VY+G+CSL+
Sbjct: 137 HAPQEQEIDSVLQVWNLATEPAFLLYAAAVVGAAIILIVQFVPQYGQSHVMVYIGVCSLV 196

Query: 184 GSLTVMSIKAIGIAIKLTLEGVSQVAYPQTWLFVTVAVVCVITQLNYLNKALDTFNAALV 243
           GSL+VMS+KA+GIA+KLT  G++Q+ YPQTW+F  + + CVITQ+NYLNKALDTFN A+V
Sbjct: 197 GSLSVMSVKALGIALKLTFSGMNQLIYPQTWVFTLIVLTCVITQMNYLNKALDTFNTAVV 256

Query: 244 SPVYYAMFTTLTIIASAIMFKDWSDQDASTIVSELCGFVTVLSGTIILHSTREQQTVSS 303
           SP+YY MFT+LTI+AS IMFKDW  QD + IV+ELCGFVT+LSGT +LH T++    SS
Sbjct: 257 SPIYYVMFTSLTILASVIMFKDWDRQDGTQIVTELCGFVTILSGTFLLHKTKDMVDGSS 315

BLAST of Lag0001460 vs. ExPASy Swiss-Prot
Match: F4JKQ7 (Probable magnesium transporter NIPA5 OS=Arabidopsis thaliana OX=3702 GN=At4g09640 PE=2 SV=1)

HSP 1 Score: 395.6 bits (1015), Expect = 5.6e-109
Identity = 193/304 (63.49%), Postives = 248/304 (81.58%), Query Frame = 0

Query: 4   SENTKGLILAVASSAFIGSSFILKKKGLKRAGATGARAGVGGYTYLLEPLWWAGMVTMII 63
           S+N KGL+LA++SS FIG+SFI+KKKGLK+AGA+G RAG GGY+YLLEPLWW GM+TMI+
Sbjct: 17  SDNVKGLVLALSSSIFIGASFIVKKKGLKKAGASGLRAGSGGYSYLLEPLWWIGMITMIV 76

Query: 64  GEIANFVAYIYAPAVLVTPLGALSIIISAVLAHFLLKERLQKMGIVGCLSCIVGSVIIVV 123
           GEIANF AY +APA+LVTPLGALSIIISA LAH +L+E+L   GI+GC  CIVGSV IV+
Sbjct: 77  GEIANFAAYAFAPAILVTPLGALSIIISASLAHIILQEKLHTFGILGCALCIVGSVTIVL 136

Query: 124 HAPQEHTPDSVEEIWDLATQPAFLVYIAATASLVLALMLYFEPRYGHVNILVYLGICSLM 183
           HAPQE    SV E+W+LAT+PAFL Y AA     + L++ F P YG  +++VY+G+CSL+
Sbjct: 137 HAPQEQDIVSVLEVWNLATEPAFLFYAAAVVGAAIVLIVQFIPLYGQSHVMVYIGVCSLI 196

Query: 184 GSLTVMSIKAIGIAIKLTLEGVSQVAYPQTWLFVTVAVVCVITQLNYLNKALDTFNAALV 243
           GSL+VMS+KA+GIA+KLT  G +Q+ YPQTW+F  + + CVITQ+NYLNKALDTFN A+V
Sbjct: 197 GSLSVMSVKALGIALKLTFSGTNQLGYPQTWVFTVIVLFCVITQMNYLNKALDTFNTAVV 256

Query: 244 SPVYYAMFTTLTIIASAIMFKDWSDQDASTIVSELCGFVTVLSGTIILHSTREQQTVSSQ 303
           SP+YY MFT+LTI+AS IMFKDW  Q  + I++ELCGFVT+LSGT +LH+T +     S+
Sbjct: 257 SPIYYVMFTSLTILASVIMFKDWDRQSGTQIMTELCGFVTILSGTFLLHTTTDMVDGESK 316

Query: 304 GSVA 308
           G+++
Sbjct: 317 GNLS 320

BLAST of Lag0001460 vs. ExPASy Swiss-Prot
Match: Q94AH3 (Probable magnesium transporter NIPA4 OS=Arabidopsis thaliana OX=3702 GN=At1g71900 PE=2 SV=1)

HSP 1 Score: 392.9 bits (1008), Expect = 3.6e-108
Identity = 190/299 (63.55%), Postives = 244/299 (81.61%), Query Frame = 0

Query: 4   SENTKGLILAVASSAFIGSSFILKKKGLKRAGATGARAGVGGYTYLLEPLWWAGMVTMII 63
           S+N KGL+LA++SS FIG+SFI+KKKGLK+A +TG RAGVGGY+YL EPLWW GM TM++
Sbjct: 17  SDNIKGLVLALSSSLFIGASFIVKKKGLKKAASTGTRAGVGGYSYLYEPLWWIGMTTMLL 76

Query: 64  GEIANFVAYIYAPAVLVTPLGALSIIISAVLAHFLLKERLQKMGIVGCLSCIVGSVIIVV 123
           GEIANF AY +APA+LVTPLGA+SIIISAVLAH +L+E+L   GI+GC  C+VGS  IV+
Sbjct: 77  GEIANFAAYAFAPAILVTPLGAVSIIISAVLAHIILREKLHIFGILGCALCVVGSTTIVL 136

Query: 124 HAPQEHTPDSVEEIWDLATQPAFLVYIAATASLVLALMLYFEPRYGHVNILVYLGICSLM 183
           HAPQE   DSV E+W+LAT+PAF+ Y +      + L++ F P+YG  N++VY+GICSL+
Sbjct: 137 HAPQEREIDSVIEVWNLATEPAFMFYASLVIGAAVFLIIRFVPQYGQTNVMVYIGICSLV 196

Query: 184 GSLTVMSIKAIGIAIKLTLEGVSQVAYPQTWLFVTVAVVCVITQLNYLNKALDTFNAALV 243
           GSL+VMS+KA+GIA+KLT  G +Q+ YPQTW+F  V + CV+TQLNYLNKALDTFN A+V
Sbjct: 197 GSLSVMSVKALGIALKLTFSGTNQLFYPQTWIFTLVVLTCVVTQLNYLNKALDTFNTAIV 256

Query: 244 SPVYYAMFTTLTIIASAIMFKDWSDQDASTIVSELCGFVTVLSGTIILHSTREQQTVSS 303
           SP+YY MFT+LTI+AS IMFKDW  Q+ + IV+E+CGFVT+LSGT +LH T++    SS
Sbjct: 257 SPIYYVMFTSLTILASVIMFKDWDRQNGTQIVTEICGFVTILSGTFLLHRTKDMVEGSS 315

BLAST of Lag0001460 vs. ExPASy TrEMBL
Match: A0A1S3BJK8 (Probable magnesium transporter OS=Cucumis melo OX=3656 GN=LOC103490304 PE=3 SV=1)

HSP 1 Score: 609.8 bits (1571), Expect = 7.0e-171
Identity = 318/333 (95.50%), Postives = 328/333 (98.50%), Query Frame = 0

Query: 1   MVISENTKGLILAVASSAFIGSSFILKKKGLKRAGATGARAGVGGYTYLLEPLWWAGMVT 60
           M ISENTKGLILA+ASSAFIGSSFILKKKGLKRAGATGARAGVGGYTYLLEPLWWAGM+T
Sbjct: 1   MTISENTKGLILAMASSAFIGSSFILKKKGLKRAGATGARAGVGGYTYLLEPLWWAGMIT 60

Query: 61  MIIGEIANFVAYIYAPAVLVTPLGALSIIISAVLAHFLLKERLQKMGIVGCLSCIVGSVI 120
           MIIGEIANFVAYIYAPAVLVTPLGALSII+SAVLAHFLLKERLQKMG+VGCLSCIVGSVI
Sbjct: 61  MIIGEIANFVAYIYAPAVLVTPLGALSIIVSAVLAHFLLKERLQKMGVVGCLSCIVGSVI 120

Query: 121 IVVHAPQEHTPDSVEEIWDLATQPAFLVYIAATASLVLALMLYFEPRYGHVNILVYLGIC 180
           IV+HAPQEHTPDSVEEIWDLATQPAFLVYIAA ASLVLALMLYFEPRYGHVNILVYLGIC
Sbjct: 121 IVIHAPQEHTPDSVEEIWDLATQPAFLVYIAAIASLVLALMLYFEPRYGHVNILVYLGIC 180

Query: 181 SLMGSLTVMSIKAIGIAIKLTLEGVSQVAYPQTWLFVTVAVVCVITQLNYLNKALDTFNA 240
           SLMGSLTVMSIKAIGIAI+LTLEG+SQVAYPQTWLFVTVAVVCVITQLNYLNKALDTFNA
Sbjct: 181 SLMGSLTVMSIKAIGIAIRLTLEGISQVAYPQTWLFVTVAVVCVITQLNYLNKALDTFNA 240

Query: 241 ALVSPVYYAMFTTLTIIASAIMFKDWSDQDASTIVSELCGFVTVLSGTIILHSTREQQTV 300
           ALVSPVYYAMFTTLTIIASAIMFKDWS Q+ASTIVSELCGF+TVLSGTIILHSTREQQ V
Sbjct: 241 ALVSPVYYAMFTTLTIIASAIMFKDWSGQNASTIVSELCGFITVLSGTIILHSTREQQPV 300

Query: 301 SSQGSVAWYITGDSMKGIEEHLITISNSHYTEE 334
           SSQGSVAWYI+GDSMK IEEHLITISNSHYTEE
Sbjct: 301 SSQGSVAWYISGDSMKSIEEHLITISNSHYTEE 333

BLAST of Lag0001460 vs. ExPASy TrEMBL
Match: A0A6J1GKB0 (Probable magnesium transporter OS=Cucurbita moschata OX=3662 GN=LOC111455145 PE=3 SV=1)

HSP 1 Score: 607.4 bits (1565), Expect = 3.5e-170
Identity = 316/332 (95.18%), Postives = 327/332 (98.49%), Query Frame = 0

Query: 1   MVISENTKGLILAVASSAFIGSSFILKKKGLKRAGATGARAGVGGYTYLLEPLWWAGMVT 60
           M ISENTKGL+LA+ASSAFIGSSFILKKKGLKRAGATGARAGVGGYTYLLEPLWWAGMVT
Sbjct: 1   MTISENTKGLVLAMASSAFIGSSFILKKKGLKRAGATGARAGVGGYTYLLEPLWWAGMVT 60

Query: 61  MIIGEIANFVAYIYAPAVLVTPLGALSIIISAVLAHFLLKERLQKMGIVGCLSCIVGSVI 120
           MIIGEIANF+AYIYAPAVLVTPLGALSIIISAVLAHFLLKERLQKMG++GCLSCIVGSVI
Sbjct: 61  MIIGEIANFIAYIYAPAVLVTPLGALSIIISAVLAHFLLKERLQKMGVLGCLSCIVGSVI 120

Query: 121 IVVHAPQEHTPDSVEEIWDLATQPAFLVYIAATASLVLALMLYFEPRYGHVNILVYLGIC 180
           IV+HAPQEHTPDSV+EIWDLATQPAFLVYIA  ASLVLALMLYFEPRYGHVNILVYLGIC
Sbjct: 121 IVIHAPQEHTPDSVQEIWDLATQPAFLVYIATIASLVLALMLYFEPRYGHVNILVYLGIC 180

Query: 181 SLMGSLTVMSIKAIGIAIKLTLEGVSQVAYPQTWLFVTVAVVCVITQLNYLNKALDTFNA 240
           SLMGSLTVMSIKAIGIAI+LTLEGVSQVAYPQTWLFVTVAVVCVITQLNYLNKALDTFNA
Sbjct: 181 SLMGSLTVMSIKAIGIAIRLTLEGVSQVAYPQTWLFVTVAVVCVITQLNYLNKALDTFNA 240

Query: 241 ALVSPVYYAMFTTLTIIASAIMFKDWSDQDASTIVSELCGFVTVLSGTIILHSTREQQTV 300
           ALVSPVYYAMFTTLTIIASAIMFKDWS Q+A+TIVSELCGF+TVLSGTIILHSTREQQ V
Sbjct: 241 ALVSPVYYAMFTTLTIIASAIMFKDWSGQNANTIVSELCGFITVLSGTIILHSTREQQPV 300

Query: 301 SSQGSVAWYITGDSMKGIEEHLITISNSHYTE 333
           SSQGSVAWYITGDSMKGIEEHLITISNSHYTE
Sbjct: 301 SSQGSVAWYITGDSMKGIEEHLITISNSHYTE 332

BLAST of Lag0001460 vs. ExPASy TrEMBL
Match: A0A0A0K5W6 (Probable magnesium transporter OS=Cucumis sativus OX=3659 GN=Csa_7G009750 PE=3 SV=1)

HSP 1 Score: 607.1 bits (1564), Expect = 4.5e-170
Identity = 317/333 (95.20%), Postives = 327/333 (98.20%), Query Frame = 0

Query: 1   MVISENTKGLILAVASSAFIGSSFILKKKGLKRAGATGARAGVGGYTYLLEPLWWAGMVT 60
           M ISEN+KGLILA+ASSAFIGSSFILKKKGLKRAGATGARAGVGGYTYLLEPLWWAGM+T
Sbjct: 1   MTISENSKGLILAMASSAFIGSSFILKKKGLKRAGATGARAGVGGYTYLLEPLWWAGMIT 60

Query: 61  MIIGEIANFVAYIYAPAVLVTPLGALSIIISAVLAHFLLKERLQKMGIVGCLSCIVGSVI 120
           MIIGEIANFVAYIYAPAVLVTPLGALSII+SAVLAHFLLKERLQKMG+VGCLSCIVGSVI
Sbjct: 61  MIIGEIANFVAYIYAPAVLVTPLGALSIIVSAVLAHFLLKERLQKMGVVGCLSCIVGSVI 120

Query: 121 IVVHAPQEHTPDSVEEIWDLATQPAFLVYIAATASLVLALMLYFEPRYGHVNILVYLGIC 180
           IV+HAPQEHTPDSVEEIWDLATQPAFLVYIAA ASLVLALMLYFEPRYGHVNILVYLGIC
Sbjct: 121 IVIHAPQEHTPDSVEEIWDLATQPAFLVYIAAIASLVLALMLYFEPRYGHVNILVYLGIC 180

Query: 181 SLMGSLTVMSIKAIGIAIKLTLEGVSQVAYPQTWLFVTVAVVCVITQLNYLNKALDTFNA 240
           SLMGSLTVMSIKAIGIAI+LTLEG+SQVAYPQTWLFVTVAVVCVITQLNYLNKALDTFNA
Sbjct: 181 SLMGSLTVMSIKAIGIAIRLTLEGISQVAYPQTWLFVTVAVVCVITQLNYLNKALDTFNA 240

Query: 241 ALVSPVYYAMFTTLTIIASAIMFKDWSDQDASTIVSELCGFVTVLSGTIILHSTREQQTV 300
           ALVSPVYYAMFTTLTIIASAIMFKDWS Q+ASTIVSELCGFVTVLSGTIILHSTREQQ V
Sbjct: 241 ALVSPVYYAMFTTLTIIASAIMFKDWSGQNASTIVSELCGFVTVLSGTIILHSTREQQPV 300

Query: 301 SSQGSVAWYITGDSMKGIEEHLITISNSHYTEE 334
           SSQGSVAWYI+GDSMK  EEHLITISNSHYTEE
Sbjct: 301 SSQGSVAWYISGDSMKSFEEHLITISNSHYTEE 333

BLAST of Lag0001460 vs. ExPASy TrEMBL
Match: A0A6J1HXZ6 (Probable magnesium transporter OS=Cucurbita maxima OX=3661 GN=LOC111468479 PE=3 SV=1)

HSP 1 Score: 603.6 bits (1555), Expect = 5.0e-169
Identity = 314/332 (94.58%), Postives = 325/332 (97.89%), Query Frame = 0

Query: 1   MVISENTKGLILAVASSAFIGSSFILKKKGLKRAGATGARAGVGGYTYLLEPLWWAGMVT 60
           M ISENTKGL+LA+ASSAFIGSSFILKKKGLKRAGATG RAGVGGYTYLLEPLWWAGMVT
Sbjct: 1   MTISENTKGLVLAMASSAFIGSSFILKKKGLKRAGATGVRAGVGGYTYLLEPLWWAGMVT 60

Query: 61  MIIGEIANFVAYIYAPAVLVTPLGALSIIISAVLAHFLLKERLQKMGIVGCLSCIVGSVI 120
           MIIGEIANF+AYIYAPAVLVTPLGALSIIISAVLAHFLLKERLQKMG++GCLSCIVGSVI
Sbjct: 61  MIIGEIANFIAYIYAPAVLVTPLGALSIIISAVLAHFLLKERLQKMGVLGCLSCIVGSVI 120

Query: 121 IVVHAPQEHTPDSVEEIWDLATQPAFLVYIAATASLVLALMLYFEPRYGHVNILVYLGIC 180
           IV+HAPQEHTPDSV+EIWDLATQPAFLVYIA  A LVLALMLYFEPRYGHVNILVYLGIC
Sbjct: 121 IVIHAPQEHTPDSVQEIWDLATQPAFLVYIATIALLVLALMLYFEPRYGHVNILVYLGIC 180

Query: 181 SLMGSLTVMSIKAIGIAIKLTLEGVSQVAYPQTWLFVTVAVVCVITQLNYLNKALDTFNA 240
           SLMGSLTVMSIKAIGIAI+LTLEGVSQVAYPQTWLFVTVAVVCVITQLNYLNKALDTFNA
Sbjct: 181 SLMGSLTVMSIKAIGIAIRLTLEGVSQVAYPQTWLFVTVAVVCVITQLNYLNKALDTFNA 240

Query: 241 ALVSPVYYAMFTTLTIIASAIMFKDWSDQDASTIVSELCGFVTVLSGTIILHSTREQQTV 300
           ALVSPVYYAMFTTLTIIASAIMFKDWS Q+A+TIVSELCGF+TVLSGTIILHSTREQQ V
Sbjct: 241 ALVSPVYYAMFTTLTIIASAIMFKDWSGQNANTIVSELCGFITVLSGTIILHSTREQQPV 300

Query: 301 SSQGSVAWYITGDSMKGIEEHLITISNSHYTE 333
           SSQGSVAWYITGDSMKGIEEHLITISNSHYTE
Sbjct: 301 SSQGSVAWYITGDSMKGIEEHLITISNSHYTE 332

BLAST of Lag0001460 vs. ExPASy TrEMBL
Match: A0A6J1EK14 (Probable magnesium transporter OS=Cucurbita moschata OX=3662 GN=LOC111435194 PE=3 SV=1)

HSP 1 Score: 603.2 bits (1554), Expect = 6.5e-169
Identity = 314/333 (94.29%), Postives = 327/333 (98.20%), Query Frame = 0

Query: 1   MVISENTKGLILAVASSAFIGSSFILKKKGLKRAGATGARAGVGGYTYLLEPLWWAGMVT 60
           M ISENTKGLILA+ASSAFIGSSFILKKKGLKRAGATGARAGVGGYTYLLEPLWWAGMV 
Sbjct: 1   MTISENTKGLILAMASSAFIGSSFILKKKGLKRAGATGARAGVGGYTYLLEPLWWAGMVI 60

Query: 61  MIIGEIANFVAYIYAPAVLVTPLGALSIIISAVLAHFLLKERLQKMGIVGCLSCIVGSVI 120
           MIIGE+ANFVAYIYAPAVLVTPLGALSII+SAVLAHFLLKERLQKMG+VGCLSCIVGSVI
Sbjct: 61  MIIGEVANFVAYIYAPAVLVTPLGALSIIVSAVLAHFLLKERLQKMGVVGCLSCIVGSVI 120

Query: 121 IVVHAPQEHTPDSVEEIWDLATQPAFLVYIAATASLVLALMLYFEPRYGHVNILVYLGIC 180
           IV+HAPQE TPDSVEEIW+LATQPAFLVY+AATASLVLALMLYFEPRYGHVNILVYLGIC
Sbjct: 121 IVIHAPQERTPDSVEEIWNLATQPAFLVYLAATASLVLALMLYFEPRYGHVNILVYLGIC 180

Query: 181 SLMGSLTVMSIKAIGIAIKLTLEGVSQVAYPQTWLFVTVAVVCVITQLNYLNKALDTFNA 240
           SLMGSLTVMSIKAIGIAI+LTLEGVSQVAYPQTWLFVTVAVVCV+TQLNYLNKALDTFNA
Sbjct: 181 SLMGSLTVMSIKAIGIAIRLTLEGVSQVAYPQTWLFVTVAVVCVVTQLNYLNKALDTFNA 240

Query: 241 ALVSPVYYAMFTTLTIIASAIMFKDWSDQDASTIVSELCGFVTVLSGTIILHSTREQQTV 300
           ALVSPVYYAMFTTLTIIASAIMFKDWS Q+ASTIVSELCGF+TVLSGT+ILHSTREQQ V
Sbjct: 241 ALVSPVYYAMFTTLTIIASAIMFKDWSGQNASTIVSELCGFITVLSGTMILHSTREQQPV 300

Query: 301 SSQGSVAWYITGDSMKGIEEHLITISNSHYTEE 334
            SQGSVAWYI+GDSMKGIEEHLITISNSHYTEE
Sbjct: 301 PSQGSVAWYISGDSMKGIEEHLITISNSHYTEE 333

BLAST of Lag0001460 vs. TAIR 10
Match: AT2G21120.1 (Protein of unknown function (DUF803) )

HSP 1 Score: 490.7 bits (1262), Expect = 9.1e-139
Identity = 253/328 (77.13%), Postives = 291/328 (88.72%), Query Frame = 0

Query: 4   SENTKGLILAVASSAFIGSSFILKKKGLKRAGATGARAGVGGYTYLLEPLWWAGMVTMII 63
           ++N KGLILAVASS FIGSSFILKKKGLKRAGA G RAG GGYTYLLEPLWWAGMVTMI+
Sbjct: 3   TDNGKGLILAVASSVFIGSSFILKKKGLKRAGAIGTRAGYGGYTYLLEPLWWAGMVTMIV 62

Query: 64  GEIANFVAYIYAPAVLVTPLGALSIIISAVLAHFLLKERLQKMGIVGCLSCIVGSVIIVV 123
           GE ANFVAYIYAPAVLVTPLGALSIIISAVLAHFLLKE+L+KMG++GC+SCIVGSV+IV+
Sbjct: 63  GEAANFVAYIYAPAVLVTPLGALSIIISAVLAHFLLKEKLKKMGVLGCVSCIVGSVVIVI 122

Query: 124 HAPQEHTPDSVEEIWDLATQPAFLVYIAATASLVLALMLYFEPRYGHVNILVYLGICSLM 183
           HAP+E TP+SVEEIW+LATQPAFL+Y+A T S+VLAL+L+FEP  G  NILVY+GICSLM
Sbjct: 123 HAPKEQTPNSVEEIWNLATQPAFLIYVAITMSIVLALILHFEPLCGQTNILVYIGICSLM 182

Query: 184 GSLTVMSIKAIGIAIKLTLEGVSQVAYPQTWLFVTVAVVCVITQLNYLNKALDTFNAALV 243
           G+LTVMSIKAIGIAIKLT+EGVSQ+ YPQTWLFV VAV CV+TQL YLNKALDTFNAA+V
Sbjct: 183 GALTVMSIKAIGIAIKLTMEGVSQIGYPQTWLFVMVAVTCVVTQLIYLNKALDTFNAAIV 242

Query: 244 SPVYYAMFTTLTIIASAIMFKDWSDQDASTIVSELCGFVTVLSGTIILHSTR-EQQTVSS 303
           SPVYY MFTTLTI+ASAIMFKDWS QDA+++ SELCGF+TVL+GT+ILH TR E+Q  +S
Sbjct: 243 SPVYYVMFTTLTIVASAIMFKDWSGQDAASVASELCGFITVLTGTMILHGTREEEQQQAS 302

Query: 304 QGSVAWYITGDSMKGIEEHLITISNSHY 331
              V WY +  SM   EEHL+++ +  Y
Sbjct: 303 SEHVRWYDSRKSMN--EEHLVSLYSPEY 328

BLAST of Lag0001460 vs. TAIR 10
Match: AT4G38730.1 (Protein of unknown function (DUF803) )

HSP 1 Score: 453.8 bits (1166), Expect = 1.2e-127
Identity = 230/329 (69.91%), Postives = 276/329 (83.89%), Query Frame = 0

Query: 2   VISENTKGLILAVASSAFIGSSFILKKKGLKRAGATGARAGVGGYTYLLEPLWWAGMVTM 61
           ++S+N  GL+LAV+SS FIGSSFILKKKGLKRA A G RAG GGYTYLLEPLWW G+VTM
Sbjct: 1   MVSDNEMGLVLAVSSSVFIGSSFILKKKGLKRAAANGTRAGFGGYTYLLEPLWWVGLVTM 60

Query: 62  IIGEIANFVAYIYAPAVLVTPLGALSIIISAVLAHFLLKERLQKMGIVGCLSCIVGSVII 121
             GEIANFVAY+YAPAVLVTPLGALSIIISAVLAHFLL E+L+KMG+ GC+ CIVGSV+I
Sbjct: 61  TFGEIANFVAYVYAPAVLVTPLGALSIIISAVLAHFLLDEKLRKMGVWGCVCCIVGSVMI 120

Query: 122 VVHAPQEHTPDSVEEIWDLATQPAFLVYIAATASLVLALMLYFEPRYGHVNILVYLGICS 181
           V+HAPQE TP+SVEEIW LA QPAFL+Y+A + S+VLAL+LY EP  G  NILVY+GICS
Sbjct: 121 VIHAPQEQTPNSVEEIWKLAMQPAFLIYVAISMSIVLALILYCEPLCGQTNILVYIGICS 180

Query: 182 LMGSLTVMSIKAIGIAIKLTLEGVSQVAYPQTWLFVTVAVVCVITQLNYLNKALDTFNAA 241
           LMGSLTVMSIKA+GIAIKLT EG++Q+ YP+TW F  VA +CV+ Q+ YLNKALDTFNAA
Sbjct: 181 LMGSLTVMSIKAVGIAIKLTFEGINQIWYPETWFFAMVAAICVVMQMIYLNKALDTFNAA 240

Query: 242 LVSPVYYAMFTTLTIIASAIMFKDWSDQDASTIVSELCGFVTVLSGTIILHSTREQQTVS 301
           +VSP+YY MFTTLTI+ASAIMFKDW+ Q+  +I SE+CGF+TVL+GT+ILHSTRE++  S
Sbjct: 241 IVSPIYYVMFTTLTIVASAIMFKDWNGQNTDSIASEICGFITVLTGTVILHSTREEEQAS 300

Query: 302 SQGSVAWYITGDSMKGIEEHLITISNSHY 331
            +  + W  +G S    EEHL ++ +  Y
Sbjct: 301 PR-RMRWQDSGKSFD--EEHLTSLYSPEY 326

BLAST of Lag0001460 vs. TAIR 10
Match: AT1G34470.1 (Protein of unknown function (DUF803) )

HSP 1 Score: 401.7 bits (1031), Expect = 5.6e-112
Identity = 193/299 (64.55%), Postives = 249/299 (83.28%), Query Frame = 0

Query: 4   SENTKGLILAVASSAFIGSSFILKKKGLKRAGATGARAGVGGYTYLLEPLWWAGMVTMII 63
           S+N KGL+LA++SS FIG+SFI+KKKGLKRAGA+G RAG GGY+YLLEPLWW GM+TMI+
Sbjct: 17  SDNIKGLVLALSSSLFIGASFIVKKKGLKRAGASGLRAGSGGYSYLLEPLWWVGMITMIV 76

Query: 64  GEIANFVAYIYAPAVLVTPLGALSIIISAVLAHFLLKERLQKMGIVGCLSCIVGSVIIVV 123
           GEIANF AY +APA+LVTPLGALSIIISA LAH +L E+L   G++GC+ C+VGS+ IV+
Sbjct: 77  GEIANFAAYAFAPAILVTPLGALSIIISAALAHVILHEKLHTFGLLGCVLCVVGSITIVL 136

Query: 124 HAPQEHTPDSVEEIWDLATQPAFLVYIAATASLVLALMLYFEPRYGHVNILVYLGICSLM 183
           HAPQE   DSV ++W+LAT+PAFL+Y AA     + L++ F P+YG  +++VY+G+CSL+
Sbjct: 137 HAPQEQEIDSVLQVWNLATEPAFLLYAAAVVGAAIILIVQFVPQYGQSHVMVYIGVCSLV 196

Query: 184 GSLTVMSIKAIGIAIKLTLEGVSQVAYPQTWLFVTVAVVCVITQLNYLNKALDTFNAALV 243
           GSL+VMS+KA+GIA+KLT  G++Q+ YPQTW+F  + + CVITQ+NYLNKALDTFN A+V
Sbjct: 197 GSLSVMSVKALGIALKLTFSGMNQLIYPQTWVFTLIVLTCVITQMNYLNKALDTFNTAVV 256

Query: 244 SPVYYAMFTTLTIIASAIMFKDWSDQDASTIVSELCGFVTVLSGTIILHSTREQQTVSS 303
           SP+YY MFT+LTI+AS IMFKDW  QD + IV+ELCGFVT+LSGT +LH T++    SS
Sbjct: 257 SPIYYVMFTSLTILASVIMFKDWDRQDGTQIVTELCGFVTILSGTFLLHKTKDMVDGSS 315

BLAST of Lag0001460 vs. TAIR 10
Match: AT4G09640.1 (Protein of unknown function (DUF803) )

HSP 1 Score: 395.6 bits (1015), Expect = 4.0e-110
Identity = 193/304 (63.49%), Postives = 248/304 (81.58%), Query Frame = 0

Query: 4   SENTKGLILAVASSAFIGSSFILKKKGLKRAGATGARAGVGGYTYLLEPLWWAGMVTMII 63
           S+N KGL+LA++SS FIG+SFI+KKKGLK+AGA+G RAG GGY+YLLEPLWW GM+TMI+
Sbjct: 17  SDNVKGLVLALSSSIFIGASFIVKKKGLKKAGASGLRAGSGGYSYLLEPLWWIGMITMIV 76

Query: 64  GEIANFVAYIYAPAVLVTPLGALSIIISAVLAHFLLKERLQKMGIVGCLSCIVGSVIIVV 123
           GEIANF AY +APA+LVTPLGALSIIISA LAH +L+E+L   GI+GC  CIVGSV IV+
Sbjct: 77  GEIANFAAYAFAPAILVTPLGALSIIISASLAHIILQEKLHTFGILGCALCIVGSVTIVL 136

Query: 124 HAPQEHTPDSVEEIWDLATQPAFLVYIAATASLVLALMLYFEPRYGHVNILVYLGICSLM 183
           HAPQE    SV E+W+LAT+PAFL Y AA     + L++ F P YG  +++VY+G+CSL+
Sbjct: 137 HAPQEQDIVSVLEVWNLATEPAFLFYAAAVVGAAIVLIVQFIPLYGQSHVMVYIGVCSLI 196

Query: 184 GSLTVMSIKAIGIAIKLTLEGVSQVAYPQTWLFVTVAVVCVITQLNYLNKALDTFNAALV 243
           GSL+VMS+KA+GIA+KLT  G +Q+ YPQTW+F  + + CVITQ+NYLNKALDTFN A+V
Sbjct: 197 GSLSVMSVKALGIALKLTFSGTNQLGYPQTWVFTVIVLFCVITQMNYLNKALDTFNTAVV 256

Query: 244 SPVYYAMFTTLTIIASAIMFKDWSDQDASTIVSELCGFVTVLSGTIILHSTREQQTVSSQ 303
           SP+YY MFT+LTI+AS IMFKDW  Q  + I++ELCGFVT+LSGT +LH+T +     S+
Sbjct: 257 SPIYYVMFTSLTILASVIMFKDWDRQSGTQIMTELCGFVTILSGTFLLHTTTDMVDGESK 316

Query: 304 GSVA 308
           G+++
Sbjct: 317 GNLS 320

BLAST of Lag0001460 vs. TAIR 10
Match: AT1G71900.1 (Protein of unknown function (DUF803) )

HSP 1 Score: 392.9 bits (1008), Expect = 2.6e-109
Identity = 190/299 (63.55%), Postives = 244/299 (81.61%), Query Frame = 0

Query: 4   SENTKGLILAVASSAFIGSSFILKKKGLKRAGATGARAGVGGYTYLLEPLWWAGMVTMII 63
           S+N KGL+LA++SS FIG+SFI+KKKGLK+A +TG RAGVGGY+YL EPLWW GM TM++
Sbjct: 17  SDNIKGLVLALSSSLFIGASFIVKKKGLKKAASTGTRAGVGGYSYLYEPLWWIGMTTMLL 76

Query: 64  GEIANFVAYIYAPAVLVTPLGALSIIISAVLAHFLLKERLQKMGIVGCLSCIVGSVIIVV 123
           GEIANF AY +APA+LVTPLGA+SIIISAVLAH +L+E+L   GI+GC  C+VGS  IV+
Sbjct: 77  GEIANFAAYAFAPAILVTPLGAVSIIISAVLAHIILREKLHIFGILGCALCVVGSTTIVL 136

Query: 124 HAPQEHTPDSVEEIWDLATQPAFLVYIAATASLVLALMLYFEPRYGHVNILVYLGICSLM 183
           HAPQE   DSV E+W+LAT+PAF+ Y +      + L++ F P+YG  N++VY+GICSL+
Sbjct: 137 HAPQEREIDSVIEVWNLATEPAFMFYASLVIGAAVFLIIRFVPQYGQTNVMVYIGICSLV 196

Query: 184 GSLTVMSIKAIGIAIKLTLEGVSQVAYPQTWLFVTVAVVCVITQLNYLNKALDTFNAALV 243
           GSL+VMS+KA+GIA+KLT  G +Q+ YPQTW+F  V + CV+TQLNYLNKALDTFN A+V
Sbjct: 197 GSLSVMSVKALGIALKLTFSGTNQLFYPQTWIFTLVVLTCVVTQLNYLNKALDTFNTAIV 256

Query: 244 SPVYYAMFTTLTIIASAIMFKDWSDQDASTIVSELCGFVTVLSGTIILHSTREQQTVSS 303
           SP+YY MFT+LTI+AS IMFKDW  Q+ + IV+E+CGFVT+LSGT +LH T++    SS
Sbjct: 257 SPIYYVMFTSLTILASVIMFKDWDRQNGTQIVTEICGFVTILSGTFLLHRTKDMVEGSS 315

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008447976.11.4e-17095.50PREDICTED: probable magnesium transporter NIPA6 isoform X1 [Cucumis melo] >XP_00... [more]
XP_022952466.17.2e-17095.18probable magnesium transporter NIPA6 isoform X1 [Cucurbita moschata] >KAG6572100... [more]
XP_004144837.39.3e-17095.20probable magnesium transporter NIPA6 isoform X1 [Cucumis sativus] >KGN43231.1 hy... [more]
KAG7011752.19.3e-17094.88putative magnesium transporter NIPA6, partial [Cucurbita argyrosperma subsp. arg... [more]
XP_038886953.11.2e-16994.89probable magnesium transporter NIPA6 isoform X1 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q8GWX21.3e-13777.13Probable magnesium transporter NIPA6 OS=Arabidopsis thaliana OX=3702 GN=At2g2112... [more]
Q8GYS11.7e-12669.91Probable magnesium transporter NIPA7 OS=Arabidopsis thaliana OX=3702 GN=At4g3873... [more]
Q9LNK77.8e-11164.55Probable magnesium transporter NIPA3 OS=Arabidopsis thaliana OX=3702 GN=At1g3447... [more]
F4JKQ75.6e-10963.49Probable magnesium transporter NIPA5 OS=Arabidopsis thaliana OX=3702 GN=At4g0964... [more]
Q94AH33.6e-10863.55Probable magnesium transporter NIPA4 OS=Arabidopsis thaliana OX=3702 GN=At1g7190... [more]
Match NameE-valueIdentityDescription
A0A1S3BJK87.0e-17195.50Probable magnesium transporter OS=Cucumis melo OX=3656 GN=LOC103490304 PE=3 SV=1[more]
A0A6J1GKB03.5e-17095.18Probable magnesium transporter OS=Cucurbita moschata OX=3662 GN=LOC111455145 PE=... [more]
A0A0A0K5W64.5e-17095.20Probable magnesium transporter OS=Cucumis sativus OX=3659 GN=Csa_7G009750 PE=3 S... [more]
A0A6J1HXZ65.0e-16994.58Probable magnesium transporter OS=Cucurbita maxima OX=3661 GN=LOC111468479 PE=3 ... [more]
A0A6J1EK146.5e-16994.29Probable magnesium transporter OS=Cucurbita moschata OX=3662 GN=LOC111435194 PE=... [more]
Match NameE-valueIdentityDescription
AT2G21120.19.1e-13977.13Protein of unknown function (DUF803) [more]
AT4G38730.11.2e-12769.91Protein of unknown function (DUF803) [more]
AT1G34470.15.6e-11264.55Protein of unknown function (DUF803) [more]
AT4G09640.14.0e-11063.49Protein of unknown function (DUF803) [more]
AT1G71900.12.6e-10963.55Protein of unknown function (DUF803) [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008521Magnesium transporter NIPAPFAMPF05653Mg_trans_NIPAcoord: 6..296
e-value: 5.9E-138
score: 459.0
IPR008521Magnesium transporter NIPAPANTHERPTHR12570UNCHARACTERIZEDcoord: 3..332
NoneNo IPR availablePANTHERPTHR12570:SF11MAGNESIUM TRANSPORTER NIPA6-RELATEDcoord: 3..332
NoneNo IPR availableSUPERFAMILY103481Multidrug resistance efflux transporter EmrEcoord: 52..124

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0001460.1Lag0001460.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:1903830 magnesium ion transmembrane transport
biological_process GO:0015693 magnesium ion transport
cellular_component GO:0005769 early endosome
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
molecular_function GO:0015095 magnesium ion transmembrane transporter activity