Lag0001453 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0001453
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionU6 small nuclear RNA (adenine-(43)-N(6))-methyltransferase
Locationchr4: 31510816 .. 31514227 (+)
RNA-Seq ExpressionLag0001453
SyntenyLag0001453
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACGAGAAAGAAGAGGAAGACGAGAGAGCCACGAACAATCCACCCCAGAAACAAATACGCTGAAAATCCCCCAGATTTCTCTCTCTTAGCCTCTCTATACCCTTCTTTTCAACCCTTCGTCTTTCTCGATCCCCGACCCAGAATCGACTGGACCGACTTCAATGCCACCCGAGAGCTTACCCGGGTTTTACTTCTCCACGACCATGCCCTTCATTGGTACTATTCTAGTTTTCTCTTTCCCCATAGCGGAAGCTTAGAAATTCTGTTGCTGTTTTGCTTTCTTTTACTTCTCTTTTCTGCTGCAACTGTGTCAGCTAAGTTAAATTTGGACTCCCATTATTGCAAAACTCTGATGGAAACTAGGGGGGGTTGGCTTATTTTTTTAGGATTTCATAGGATTATGGCTAGGCTTGTTTTATCCCTTTTGAAGTGATTAGGAGATATGCTAACCCAGGTCATTGTTTTTAAGAGGATCAACACTGTATACCGTATGTGGGTCATTGTTATTTTTCAAGTTTGAACTTGTTTCAAATTGTGTCTCTTGATTTGAGGAATCATGTGTTTTGACAGTTCTTTCATTGTGTTTCGCTTGTTGGTTTGTTGGCGATGTGAAAAGGCTTTGTTTGTCAAATTAGGATGGCTTTGTGATTGAAGTGTAAGTAGATCTCTACATTTCAGGTGGATTCCTGATGGGCAGCTCTGCCCAACGGTACCTAATAGATCAAATTATATCCATTGGCTTGAAGATCTTTTGTCATCTGATATCATTGCAAAGGCAAATAGTGATAGAGGTCGTGTAAGAGGTTTTGATATAGGAACTGGAGCAAATTGCATTTATCCACTTCTTGGAGCCTCTCTTCTCGGGTGGAGTTTCGTTGGGACAGGTGACTTCCTTACCATATAGTTGGTTTTGTTCTCCAAATTCTATATTACCCATTGATTTGTATGTTTAACAATGACCAGGTTTTGGGTTCCGCTCTGCTTGGTGCAGATGTGACTGATGTAGCACTAGAGTGGGCTGAAAGAAATGTTAAAAGTAATCCACACGTTTCTGAACTTATTGAAATTAGAAAGGTAGATAGTACCTTGGACGGTCTTACTACAGAATTACATAATGGTGTAGCCGTTGAAAGTGAAAGCAAGAATTTAAATGATATAGATGGTAAGGAGGTACGGCTGCCGTTGCCCTCTTCCTCATTAAAGGAGACAGTCTACTTGGAGAAGAGATATCATGGTCCTCCCATACTTCTTGGTGTTGTGAAAGATGGCGAGGAGTTTGACTTTTGCATGTGCAATCCTCCCTTTTTTGAGAGTATGGATGAAGCAGGGTTGAATCCGAAGACTTCTTGTGGCGGAACTCCTCAAGAGATGGTTTGTCCAGGTGGGGAGAGGGCGTTTATTAGCCGTATGATTGAAGACAGCACTGTCTTGAAGCAGACTTTCCGGTATTCCTTGCTCTTACAACTGCAAAGTTGCCTCTGATTCTTCTCTATACTTTTTAGTAATCTTATCCTTTTATCTTCATTGATATTTGCAGGTGGTATACTTCAATGATTGGGAAGAAATCAAACCTCAGCTTCCTGATATCTAAACTTTGGAAAGTTGGAGTGACAGTCGTCAAGACTACAGAATTTGTCCAAGGCCAAACTTGCCGATGGGGACTTGCTTGGTCTTTTATGCCTGCAGCACGGAAGATTATATCTCCTCATGTTACTAACAAGAGTGTCTTGTCTTTCATGCTCGAGGTGGATAACTGATTGAGCTTCTTAAATTTTTTTTTGTTTTTTGTATTGGGCTTACACAGACCTCAAATTCGTTGATCAAATCTTTTCTATGTTTGTTGAGATATTAATCCCTTTATGTAGATATGTAGCTCAGTTTTGATATCTTTCTATTGCTCAGGGCCTCCTTCCACAAGTCAGTGCCATGAGTATACTGCAATCAGTTGAACTTTTTCTGAGCACCGGTGGAGCTTCTTGTAAACTGAACACTTCTTCATTTACAGTAGATGTATGGTTGTTCAATTGGCTTCTTTTATATCTTTGCAAGTGATGATCATGCTAACAAGTTACATTACAACAAATGTTTTTGTAGATTACTGCATCTGAGGATCATTGCCAAGCAATTCTCAAGAATGGGGCATTCGAGGTGGACAAAGGTGCTGGTTGTGTAACTGTGGAAAAATCAGAGCCTCAACTCTCGAGTGATCTGTGTTTTCGAGTTTCGGTATTTTCCTGCTTGTCCATTTATGGTCGTGGAACTTCATTACTAATGCTTTGCCCATACTTTTGACTATCGCAATTTTCTTTGTTATAGGTATTTCAGCAAATCCCTGGCACGCTTCTTGTGAAAGGCTCCTTGCATCATAGGAGTAGTCCTTCTGCAGGTACTACTCGCATATGCATTTCAACTTTCTTTTGATTGTAATTTATGTGGACTTCCACAAAAACTATAGAAATGAATTTTTGTTTTTTAAATATTATTTTAAAAGAAACTATTGATTAGTATTACCAACAAATTGAACTAAATGAAAGCTCAGTTTCTCAAAGAAGAAAAAAACAAAACAGATTACAAACAAGAATACAAGAGATTTCCTGGGAGTGAAAGAGCTTTTAATCTATTTTCAACACTCTCTTTCACGCATTTGACTCAAATATTTGACAAGGATCAACACTGGAACTGTTATTAGGAAATAGAGAGAAAATAACCTCCTACTCTTGTATGGCTAAGCTTTTGGCTCTTTTAATTTTTTCAATATTATATTCTCAAGAGTTACAAGAAGAAACCCAACTGCCAAGAATTAAAATGCGATTTTGTATATATCTAATATGCATGATGCAACGAAATAGCAATATGACCACGGTGTATATAATTCCCCTTGCAGGCTTATTCTCACTGATTTTCCACCGATTAGAGGAATTCCTGAAAGCTGAATCTTGTAAAGAAAAGGTGTTATAGAGATCCCTCTGCCTCATCCAACAATACTTTTTATGTAGAAGCTCCACAACAGGGCAACTGTATTGCTTGCTATGCAGGGGCAGAGCGAAGAAAGGAGGGAGCAATGTTGTAATTCAAAATCTATATAGTTTTGTAGTTTTCCTCTAATTTTCTCTTTTAATTTTTGGAAGTTAAAAGATTATTATTTTTGGATCTTTTGAAGCTTGAATTCTAGTCTTCTGCAAGAACAGTCACAGTTTACAGTAGGTAAGCCTTGAAACTACCATTGTATTTTGTTTGTTTCCAATACATAGCCCATGAACTATGCGCTGGACCCGCAATTGTACACTTTATGTCATAGAGCAATGAAGGATCTTGATCATATTTTGAGAGGCACAGGTGCTGGAAATCCTTTGGGATTAGTTGGATACGAAGCAGAGGTGTTGTTTCTCTGTTCGAAGAGATTCTTATGA

mRNA sequence

ATGACGAGAAAGAAGAGGAAGACGAGAGAGCCACGAACAATCCACCCCAGAAACAAATACGCTGAAAATCCCCCAGATTTCTCTCTCTTAGCCTCTCTATACCCTTCTTTTCAACCCTTCGTCTTTCTCGATCCCCGACCCAGAATCGACTGGACCGACTTCAATGCCACCCGAGAGCTTACCCGGGTTTTACTTCTCCACGACCATGCCCTTCATTGGTGGATTCCTGATGGGCAGCTCTGCCCAACGGTACCTAATAGATCAAATTATATCCATTGGCTTGAAGATCTTTTGTCATCTGATATCATTGCAAAGGCAAATAGTGATAGAGGTCGTGTAAGAGGTTTTGATATAGGAACTGGAGCAAATTGCATTTATCCACTTCTTGGAGCCTCTCTTCTCGGGTGGAGTTTCGTTGGGACAGATGTGACTGATGTAGCACTAGAGTGGGCTGAAAGAAATGTTAAAAGTAATCCACACGTTTCTGAACTTATTGAAATTAGAAAGGTAGATAGTACCTTGGACGGTCTTACTACAGAATTACATAATGGTGTAGCCGTTGAAAGTGAAAGCAAGAATTTAAATGATATAGATGGTAAGGAGGTACGGCTGCCGTTGCCCTCTTCCTCATTAAAGGAGACAGTCTACTTGGAGAAGAGATATCATGGTCCTCCCATACTTCTTGGTGTTGTGAAAGATGGCGAGGAGTTTGACTTTTGCATGTGCAATCCTCCCTTTTTTGAGAGTATGGATGAAGCAGGGTTGAATCCGAAGACTTCTTGTGGCGGAACTCCTCAAGAGATGGTTTGTCCAGGTGGGGAGAGGGCGTTTATTAGCCGTATGATTGAAGACAGCACTGTCTTGAAGCAGACTTTCCGGTGGTATACTTCAATGATTGGGAAGAAATCAAACCTCAGCTTCCTGATATCTAAACTTTGGAAAGTTGGAGTGACAGTCGTCAAGACTACAGAATTTGTCCAAGGCCAAACTTGCCGATGGGGACTTGCTTGGTCTTTTATGCCTGCAGCACGGAAGATTATATCTCCTCATGTTACTAACAAGAGTGTCTTGTCTTTCATGCTCGAGGGCCTCCTTCCACAAGTCAGTGCCATGAGTATACTGCAATCAGTTGAACTTTTTCTGAGCACCGGTGGAGCTTCTTGTAAACTGAACACTTCTTCATTTACAGTAGATATTACTGCATCTGAGGATCATTGCCAAGCAATTCTCAAGAATGGGGCATTCGAGGTGGACAAAGGTGCTGGTTGTGTAACTGTGGAAAAATCAGAGCCTCAACTCTCGAGTGATCTGTGTTTTCGAGTTTCGGTATTTCAGCAAATCCCTGGCACGCTTCTTGTGAAAGGCTCCTTGCATCATAGGAGTAGTCCTTCTGCAGGCTTATTCTCACTGATTTTCCACCGATTAGAGGAATTCCTGAAAGCTGAATCTTGTAAAGAAAAGCCCATGAACTATGCGCTGGACCCGCAATTGTACACTTTATGTCATAGAGCAATGAAGGATCTTGATCATATTTTGAGAGGCACAGGTGCTGGAAATCCTTTGGGATTAGTTGGATACGAAGCAGAGGTGTTGTTTCTCTGTTCGAAGAGATTCTTATGA

Coding sequence (CDS)

ATGACGAGAAAGAAGAGGAAGACGAGAGAGCCACGAACAATCCACCCCAGAAACAAATACGCTGAAAATCCCCCAGATTTCTCTCTCTTAGCCTCTCTATACCCTTCTTTTCAACCCTTCGTCTTTCTCGATCCCCGACCCAGAATCGACTGGACCGACTTCAATGCCACCCGAGAGCTTACCCGGGTTTTACTTCTCCACGACCATGCCCTTCATTGGTGGATTCCTGATGGGCAGCTCTGCCCAACGGTACCTAATAGATCAAATTATATCCATTGGCTTGAAGATCTTTTGTCATCTGATATCATTGCAAAGGCAAATAGTGATAGAGGTCGTGTAAGAGGTTTTGATATAGGAACTGGAGCAAATTGCATTTATCCACTTCTTGGAGCCTCTCTTCTCGGGTGGAGTTTCGTTGGGACAGATGTGACTGATGTAGCACTAGAGTGGGCTGAAAGAAATGTTAAAAGTAATCCACACGTTTCTGAACTTATTGAAATTAGAAAGGTAGATAGTACCTTGGACGGTCTTACTACAGAATTACATAATGGTGTAGCCGTTGAAAGTGAAAGCAAGAATTTAAATGATATAGATGGTAAGGAGGTACGGCTGCCGTTGCCCTCTTCCTCATTAAAGGAGACAGTCTACTTGGAGAAGAGATATCATGGTCCTCCCATACTTCTTGGTGTTGTGAAAGATGGCGAGGAGTTTGACTTTTGCATGTGCAATCCTCCCTTTTTTGAGAGTATGGATGAAGCAGGGTTGAATCCGAAGACTTCTTGTGGCGGAACTCCTCAAGAGATGGTTTGTCCAGGTGGGGAGAGGGCGTTTATTAGCCGTATGATTGAAGACAGCACTGTCTTGAAGCAGACTTTCCGGTGGTATACTTCAATGATTGGGAAGAAATCAAACCTCAGCTTCCTGATATCTAAACTTTGGAAAGTTGGAGTGACAGTCGTCAAGACTACAGAATTTGTCCAAGGCCAAACTTGCCGATGGGGACTTGCTTGGTCTTTTATGCCTGCAGCACGGAAGATTATATCTCCTCATGTTACTAACAAGAGTGTCTTGTCTTTCATGCTCGAGGGCCTCCTTCCACAAGTCAGTGCCATGAGTATACTGCAATCAGTTGAACTTTTTCTGAGCACCGGTGGAGCTTCTTGTAAACTGAACACTTCTTCATTTACAGTAGATATTACTGCATCTGAGGATCATTGCCAAGCAATTCTCAAGAATGGGGCATTCGAGGTGGACAAAGGTGCTGGTTGTGTAACTGTGGAAAAATCAGAGCCTCAACTCTCGAGTGATCTGTGTTTTCGAGTTTCGGTATTTCAGCAAATCCCTGGCACGCTTCTTGTGAAAGGCTCCTTGCATCATAGGAGTAGTCCTTCTGCAGGCTTATTCTCACTGATTTTCCACCGATTAGAGGAATTCCTGAAAGCTGAATCTTGTAAAGAAAAGCCCATGAACTATGCGCTGGACCCGCAATTGTACACTTTATGTCATAGAGCAATGAAGGATCTTGATCATATTTTGAGAGGCACAGGTGCTGGAAATCCTTTGGGATTAGTTGGATACGAAGCAGAGGTGTTGTTTCTCTGTTCGAAGAGATTCTTATGA

Protein sequence

MTRKKRKTREPRTIHPRNKYAENPPDFSLLASLYPSFQPFVFLDPRPRIDWTDFNATRELTRVLLLHDHALHWWIPDGQLCPTVPNRSNYIHWLEDLLSSDIIAKANSDRGRVRGFDIGTGANCIYPLLGASLLGWSFVGTDVTDVALEWAERNVKSNPHVSELIEIRKVDSTLDGLTTELHNGVAVESESKNLNDIDGKEVRLPLPSSSLKETVYLEKRYHGPPILLGVVKDGEEFDFCMCNPPFFESMDEAGLNPKTSCGGTPQEMVCPGGERAFISRMIEDSTVLKQTFRWYTSMIGKKSNLSFLISKLWKVGVTVVKTTEFVQGQTCRWGLAWSFMPAARKIISPHVTNKSVLSFMLEGLLPQVSAMSILQSVELFLSTGGASCKLNTSSFTVDITASEDHCQAILKNGAFEVDKGAGCVTVEKSEPQLSSDLCFRVSVFQQIPGTLLVKGSLHHRSSPSAGLFSLIFHRLEEFLKAESCKEKPMNYALDPQLYTLCHRAMKDLDHILRGTGAGNPLGLVGYEAEVLFLCSKRFL
Homology
BLAST of Lag0001453 vs. NCBI nr
Match: XP_022969493.1 (U6 small nuclear RNA (adenine-(43)-N(6))-methyltransferase [Cucurbita maxima])

HSP 1 Score: 901.7 bits (2329), Expect = 3.0e-258
Identity = 439/488 (89.96%), Postives = 459/488 (94.06%), Query Frame = 0

Query: 1   MTRKKRKTREPRTIHPRNKYAENPPDFSLLASLYPSFQPFVFLDPRPRIDWTDFNATREL 60
           MTRKKRKTRE  TIHPRNKYAENPPDF+LLASLYPSFQPFVFL+PRPRIDWTDFNATREL
Sbjct: 1   MTRKKRKTREAATIHPRNKYAENPPDFALLASLYPSFQPFVFLEPRPRIDWTDFNATREL 60

Query: 61  TRVLLLHDHALHWWIPDGQLCPTVPNRSNYIHWLEDLLSSDIIAKANSDRG-RVRGFDIG 120
           TRVLLLHDHALHWWIPDGQLCPTVPNRSNYIHWLEDLLSSD++AKAN DRG  VRGFDIG
Sbjct: 61  TRVLLLHDHALHWWIPDGQLCPTVPNRSNYIHWLEDLLSSDVVAKANRDRGSNVRGFDIG 120

Query: 121 TGANCIYPLLGASLLGWSFVGTDVTDVALEWAERNVKSNPHVSELIEIRKVDSTLDGLTT 180
           TGANCIYPLLGASLLGWSFVGTDVTDVALEWAERNVKSNPHVSELIEIRKVD TLDGLTT
Sbjct: 121 TGANCIYPLLGASLLGWSFVGTDVTDVALEWAERNVKSNPHVSELIEIRKVDDTLDGLTT 180

Query: 181 ELHNGVAVESESKNLNDIDGKEVRLPLPSSSLKETVYLEKRYHGPPILLGVVKDGEEFDF 240
           EL + VAV++E K +N +DG+EV    PSSS KET++ EKRYHGPPILLGVVKDGEEFDF
Sbjct: 181 ELRSNVAVDNERKIMNGMDGREVGPSPPSSSSKETIHSEKRYHGPPILLGVVKDGEEFDF 240

Query: 241 CMCNPPFFESMDEAGLNPKTSCGGTPQEMVCPGGERAFISRMIEDSTVLKQTFRWYTSMI 300
           CMCNPPFFESMDEAGLNPKT+CGGTPQEMVCPGGERAFISRMIEDS VLKQTFRWYTSMI
Sbjct: 241 CMCNPPFFESMDEAGLNPKTACGGTPQEMVCPGGERAFISRMIEDSIVLKQTFRWYTSMI 300

Query: 301 GKKSNLSFLISKLWKVGVTVVKTTEFVQGQTCRWGLAWSFMPAARKIISPHVTNKSVLSF 360
           GKKSNLSFLISKLWKVGVTVVKTTEFVQGQTCRWGLAWSFMPA RKIISPHVTNKSV SF
Sbjct: 301 GKKSNLSFLISKLWKVGVTVVKTTEFVQGQTCRWGLAWSFMPAGRKIISPHVTNKSVSSF 360

Query: 361 MLEGLLPQVSAMSILQSVELFLSTGGASCKLNTSSFTVDITASEDHCQAILKNGAFEVDK 420
           MLEGLLPQVSAMSILQSVE FLS GGASCKLNTSSFTVD+TAS DHC+AIL+NGA+EVDK
Sbjct: 361 MLEGLLPQVSAMSILQSVERFLSAGGASCKLNTSSFTVDVTASNDHCRAILENGAYEVDK 420

Query: 421 GAGCVTVEKSEPQLSSDLCFRVSVFQQIPGTLLVKGSLHHRSSPSAGLFSLIFHRLEEFL 480
           GA  V VEKSEPQ S+DLCFRVSVFQQIPGTLLVKGSLHH+SSPS+G FS IFHRLEEFL
Sbjct: 421 GASGVPVEKSEPQPSTDLCFRVSVFQQIPGTLLVKGSLHHKSSPSSGSFSQIFHRLEEFL 480

Query: 481 KAESCKEK 488
           KAESCKEK
Sbjct: 481 KAESCKEK 488

BLAST of Lag0001453 vs. NCBI nr
Match: XP_038886852.1 (U6 small nuclear RNA (adenine-(43)-N(6))-methyltransferase isoform X2 [Benincasa hispida])

HSP 1 Score: 898.3 bits (2320), Expect = 3.3e-257
Identity = 441/487 (90.55%), Postives = 452/487 (92.81%), Query Frame = 0

Query: 1   MTRKKRKTREPRTIHPRNKYAENPPDFSLLASLYPSFQPFVFLDPRPRIDWTDFNATREL 60
           MTRKKRKTRE  TIHPRNKYAENPPDF+LLASLYPSFQPFVFL PRPRIDWTDFNATREL
Sbjct: 1   MTRKKRKTREAATIHPRNKYAENPPDFALLASLYPSFQPFVFLHPRPRIDWTDFNATREL 60

Query: 61  TRVLLLHDHALHWWIPDGQLCPTVPNRSNYIHWLEDLLSSDIIAKANSDRGRVRGFDIGT 120
           TRVLLLHDHALHWWIPDGQLCPTVPNRSNYIHWLEDLLSSDI+AK NSD G VRG DIGT
Sbjct: 61  TRVLLLHDHALHWWIPDGQLCPTVPNRSNYIHWLEDLLSSDIVAKKNSDSGPVRGLDIGT 120

Query: 121 GANCIYPLLGASLLGWSFVGTDVTDVALEWAERNVKSNPHVSELIEIRKVDSTLDGLTTE 180
           GANCIYPLLGASLLGWSFVGTDVTDVALEWAERNVKSNPHVSELIEIRKVD   D L+TE
Sbjct: 121 GANCIYPLLGASLLGWSFVGTDVTDVALEWAERNVKSNPHVSELIEIRKVDDISDDLSTE 180

Query: 181 LHNGVAVESESKNLNDIDGKEVRLPLPSSSLKETVYLEKRYHGPPILLGVVKDGEEFDFC 240
           LHN VAV+SE K LN+ DG EV  PLPSS  KETV+LEKRYHGPPILLGVVKDGEEFDFC
Sbjct: 181 LHNSVAVDSEFKILNEKDGMEVAPPLPSSLSKETVHLEKRYHGPPILLGVVKDGEEFDFC 240

Query: 241 MCNPPFFESMDEAGLNPKTSCGGTPQEMVCPGGERAFISRMIEDSTVLKQTFRWYTSMIG 300
           MCNPPFFESMDEAGLNPKTSCGGTPQEMVCPGGERAFISRMIEDS VL+QTFRWYTSMIG
Sbjct: 241 MCNPPFFESMDEAGLNPKTSCGGTPQEMVCPGGERAFISRMIEDSIVLRQTFRWYTSMIG 300

Query: 301 KKSNLSFLISKLWKVGVTVVKTTEFVQGQTCRWGLAWSFMPAARKIISPHVTNKSVLSFM 360
           KKSNLSFLISKLWKVGVTVVKTTEFVQGQ CRWGLAWSFMP ARKIISPHVTNKSV+SFM
Sbjct: 301 KKSNLSFLISKLWKVGVTVVKTTEFVQGQICRWGLAWSFMPTARKIISPHVTNKSVMSFM 360

Query: 361 LEGLLPQVSAMSILQSVELFLSTGGASCKLNTSSFTVDITASEDHCQAILKNGAFEVDKG 420
           LEGLLPQVSA SILQSVE FLS  GASCKLNTSSFTVDITASEDHC+AILKN A EVDKG
Sbjct: 361 LEGLLPQVSATSILQSVERFLSASGASCKLNTSSFTVDITASEDHCRAILKNRACEVDKG 420

Query: 421 AGCVTVEKSEPQLSSDLCFRVSVFQQIPGTLLVKGSLHHRSSPSAGLFSLIFHRLEEFLK 480
           A   TVEKSEPQ SSDLCFRVSVFQQIPG+LLVKGSLHH+SSPSAG FSLIFH LEEFLK
Sbjct: 421 ASSATVEKSEPQPSSDLCFRVSVFQQIPGSLLVKGSLHHKSSPSAGSFSLIFHGLEEFLK 480

Query: 481 AESCKEK 488
           AESCKEK
Sbjct: 481 AESCKEK 487

BLAST of Lag0001453 vs. NCBI nr
Match: KAG6572103.1 (U6 small nuclear RNA (adenine-(43)-N(6))-methyltransferase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 897.9 bits (2319), Expect = 4.3e-257
Identity = 437/488 (89.55%), Postives = 458/488 (93.85%), Query Frame = 0

Query: 1   MTRKKRKTREPRTIHPRNKYAENPPDFSLLASLYPSFQPFVFLDPRPRIDWTDFNATREL 60
           MTRKKRKTRE  TIHPRNKYAENPPDF+LLASLYPSFQPFVFL+PRPRIDWTDFNATREL
Sbjct: 1   MTRKKRKTREAATIHPRNKYAENPPDFALLASLYPSFQPFVFLEPRPRIDWTDFNATREL 60

Query: 61  TRVLLLHDHALHWWIPDGQLCPTVPNRSNYIHWLEDLLSSDIIAKANSDRG-RVRGFDIG 120
           TRVLLLHDHALHWWIPDGQLCPTVPNRSNYIHWLEDLLSSD++AKANSDRG  VRGFDIG
Sbjct: 61  TRVLLLHDHALHWWIPDGQLCPTVPNRSNYIHWLEDLLSSDVVAKANSDRGSNVRGFDIG 120

Query: 121 TGANCIYPLLGASLLGWSFVGTDVTDVALEWAERNVKSNPHVSELIEIRKVDSTLDGLTT 180
           TGANCIYPLLGASLLGWSFVGTDVTDVALEWAERNVKSNPHVSELIEIRKVD TLDGLTT
Sbjct: 121 TGANCIYPLLGASLLGWSFVGTDVTDVALEWAERNVKSNPHVSELIEIRKVDDTLDGLTT 180

Query: 181 ELHNGVAVESESKNLNDIDGKEVRLPLPSSSLKETVYLEKRYHGPPILLGVVKDGEEFDF 240
           EL + VAV++E K +N +DG+EV    PSSS KET++ EKRYHGPPILLGVVKDGEEFDF
Sbjct: 181 ELRSNVAVDNECKIMNGMDGREVAPSPPSSSSKETIHSEKRYHGPPILLGVVKDGEEFDF 240

Query: 241 CMCNPPFFESMDEAGLNPKTSCGGTPQEMVCPGGERAFISRMIEDSTVLKQTFRWYTSMI 300
           CMCNPPFFESMDEAGLNPKT+CGGTPQEMVCPGGERAFISRMIEDS VL+QTFRWYTSMI
Sbjct: 241 CMCNPPFFESMDEAGLNPKTACGGTPQEMVCPGGERAFISRMIEDSIVLRQTFRWYTSMI 300

Query: 301 GKKSNLSFLISKLWKVGVTVVKTTEFVQGQTCRWGLAWSFMPAARKIISPHVTNKSVLSF 360
           GKKSNLSFLISKLWKVGVTVVKTTEFVQGQTCRWGLAWSFMPA RKIISPHVTNKSV SF
Sbjct: 301 GKKSNLSFLISKLWKVGVTVVKTTEFVQGQTCRWGLAWSFMPAGRKIISPHVTNKSVSSF 360

Query: 361 MLEGLLPQVSAMSILQSVELFLSTGGASCKLNTSSFTVDITASEDHCQAILKNGAFEVDK 420
           MLEGLLPQVSA SILQSVE FLS GGASCKLNTSSFTVD+TAS  HC+AIL+NGA+EVDK
Sbjct: 361 MLEGLLPQVSATSILQSVERFLSVGGASCKLNTSSFTVDVTASNAHCRAILENGAYEVDK 420

Query: 421 GAGCVTVEKSEPQLSSDLCFRVSVFQQIPGTLLVKGSLHHRSSPSAGLFSLIFHRLEEFL 480
           GA  V VEKSEPQ S+DLCFRVSVFQQIPGTLLVKGSLHH+SSPS+G FS IFHRLEEFL
Sbjct: 421 GASGVAVEKSEPQPSTDLCFRVSVFQQIPGTLLVKGSLHHKSSPSSGSFSQIFHRLEEFL 480

Query: 481 KAESCKEK 488
           KAESCKEK
Sbjct: 481 KAESCKEK 488

BLAST of Lag0001453 vs. NCBI nr
Match: XP_022952458.1 (U6 small nuclear RNA (adenine-(43)-N(6))-methyltransferase [Cucurbita moschata])

HSP 1 Score: 897.5 bits (2318), Expect = 5.6e-257
Identity = 437/488 (89.55%), Postives = 458/488 (93.85%), Query Frame = 0

Query: 1   MTRKKRKTREPRTIHPRNKYAENPPDFSLLASLYPSFQPFVFLDPRPRIDWTDFNATREL 60
           MTRKKRKTRE  TIHPRNKYAENPPDF+LLASLYPSFQPFVFL+PRPRIDWTDFNATREL
Sbjct: 1   MTRKKRKTREAATIHPRNKYAENPPDFALLASLYPSFQPFVFLEPRPRIDWTDFNATREL 60

Query: 61  TRVLLLHDHALHWWIPDGQLCPTVPNRSNYIHWLEDLLSSDIIAKANSDRG-RVRGFDIG 120
           TRVLLLHDHALHWWIPDGQLCPTVPNRSNYIHWLEDLLSSD++AKANSDRG  VRGFDIG
Sbjct: 61  TRVLLLHDHALHWWIPDGQLCPTVPNRSNYIHWLEDLLSSDVVAKANSDRGSNVRGFDIG 120

Query: 121 TGANCIYPLLGASLLGWSFVGTDVTDVALEWAERNVKSNPHVSELIEIRKVDSTLDGLTT 180
           TGANCIYPLLGASLLGWSFVGTDVT+VALEWAERNVKSNPHVSELIEIRKVD TLDGLT 
Sbjct: 121 TGANCIYPLLGASLLGWSFVGTDVTNVALEWAERNVKSNPHVSELIEIRKVDDTLDGLTI 180

Query: 181 ELHNGVAVESESKNLNDIDGKEVRLPLPSSSLKETVYLEKRYHGPPILLGVVKDGEEFDF 240
           EL + VAV++E K +N +DG+EV    PSSS KET++ EKRYHGPPILLGVVKDGEEFDF
Sbjct: 181 ELRSNVAVDNECKIMNGMDGREVGPSPPSSSSKETIHSEKRYHGPPILLGVVKDGEEFDF 240

Query: 241 CMCNPPFFESMDEAGLNPKTSCGGTPQEMVCPGGERAFISRMIEDSTVLKQTFRWYTSMI 300
           CMCNPPFFESMDEAGLNPKT+CGGTPQEMVCPGGERAFISRMIEDS VLKQTFRWYTSMI
Sbjct: 241 CMCNPPFFESMDEAGLNPKTACGGTPQEMVCPGGERAFISRMIEDSIVLKQTFRWYTSMI 300

Query: 301 GKKSNLSFLISKLWKVGVTVVKTTEFVQGQTCRWGLAWSFMPAARKIISPHVTNKSVLSF 360
           GKKSNLSFLISKLWKVGVTVVKTTEFVQGQTCRWGLAWSFMPA RKIISPHVTNKSV SF
Sbjct: 301 GKKSNLSFLISKLWKVGVTVVKTTEFVQGQTCRWGLAWSFMPAGRKIISPHVTNKSVSSF 360

Query: 361 MLEGLLPQVSAMSILQSVELFLSTGGASCKLNTSSFTVDITASEDHCQAILKNGAFEVDK 420
           MLEGLLPQVSA SILQSVE FLS GGASCKLNTSSFTVD+TAS DHC+AIL+NGA+EVDK
Sbjct: 361 MLEGLLPQVSATSILQSVERFLSVGGASCKLNTSSFTVDVTASNDHCRAILENGAYEVDK 420

Query: 421 GAGCVTVEKSEPQLSSDLCFRVSVFQQIPGTLLVKGSLHHRSSPSAGLFSLIFHRLEEFL 480
           GA  V VEKSEPQ S+DLCFRVSVFQQIPGTLLVKGSLHH+SSPS+G FS IFHRLEEFL
Sbjct: 421 GASGVAVEKSEPQPSADLCFRVSVFQQIPGTLLVKGSLHHKSSPSSGSFSQIFHRLEEFL 480

Query: 481 KAESCKEK 488
           KAESCKEK
Sbjct: 481 KAESCKEK 488

BLAST of Lag0001453 vs. NCBI nr
Match: XP_023554274.1 (U6 small nuclear RNA (adenine-(43)-N(6))-methyltransferase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 895.6 bits (2313), Expect = 2.1e-256
Identity = 436/488 (89.34%), Postives = 457/488 (93.65%), Query Frame = 0

Query: 1   MTRKKRKTREPRTIHPRNKYAENPPDFSLLASLYPSFQPFVFLDPRPRIDWTDFNATREL 60
           MTRKKRKTRE  TIHPRNKYAENPPDF+LLASLYPSFQPFVFL+PRPRIDWTDFNATREL
Sbjct: 1   MTRKKRKTREAATIHPRNKYAENPPDFALLASLYPSFQPFVFLEPRPRIDWTDFNATREL 60

Query: 61  TRVLLLHDHALHWWIPDGQLCPTVPNRSNYIHWLEDLLSSDIIAKANSDRG-RVRGFDIG 120
           TRVLLLHDHALHWWIPDGQLCPTVPNRSNYIHWLEDLLSSD++AKANSDRG  VRGFDIG
Sbjct: 61  TRVLLLHDHALHWWIPDGQLCPTVPNRSNYIHWLEDLLSSDVVAKANSDRGSNVRGFDIG 120

Query: 121 TGANCIYPLLGASLLGWSFVGTDVTDVALEWAERNVKSNPHVSELIEIRKVDSTLDGLTT 180
           TGANCIYPLLGASLLGWSFVGTDVTDVALEWAERNVKSNPHVSELIEIRKVD TLDGLTT
Sbjct: 121 TGANCIYPLLGASLLGWSFVGTDVTDVALEWAERNVKSNPHVSELIEIRKVDDTLDGLTT 180

Query: 181 ELHNGVAVESESKNLNDIDGKEVRLPLPSSSLKETVYLEKRYHGPPILLGVVKDGEEFDF 240
           EL + VAV++E K +N +DG+EV    PSSS KET++ EKRYHGPPILLGVVKDGEEFDF
Sbjct: 181 ELRSNVAVDNECKIMNGMDGREVGPSPPSSSSKETIHSEKRYHGPPILLGVVKDGEEFDF 240

Query: 241 CMCNPPFFESMDEAGLNPKTSCGGTPQEMVCPGGERAFISRMIEDSTVLKQTFRWYTSMI 300
           CMCNPPFFESMDEAGLNPKT+CGGTPQEMVCPGGERAFISRMIEDS VLKQTFRWYTSMI
Sbjct: 241 CMCNPPFFESMDEAGLNPKTACGGTPQEMVCPGGERAFISRMIEDSIVLKQTFRWYTSMI 300

Query: 301 GKKSNLSFLISKLWKVGVTVVKTTEFVQGQTCRWGLAWSFMPAARKIISPHVTNKSVLSF 360
           GKKSNLSFLISKLWKVGVTVVKTTEFVQGQTCRWGLAWSFMPAARKIISPHVTNKSV SF
Sbjct: 301 GKKSNLSFLISKLWKVGVTVVKTTEFVQGQTCRWGLAWSFMPAARKIISPHVTNKSVSSF 360

Query: 361 MLEGLLPQVSAMSILQSVELFLSTGGASCKLNTSSFTVDITASEDHCQAILKNGAFEVDK 420
           MLEGLLPQVSA SILQSVE FLS GGASCKLN SSFTVD+TAS DHC+ IL+NGA+EVDK
Sbjct: 361 MLEGLLPQVSASSILQSVERFLSAGGASCKLNISSFTVDVTASNDHCREILENGAYEVDK 420

Query: 421 GAGCVTVEKSEPQLSSDLCFRVSVFQQIPGTLLVKGSLHHRSSPSAGLFSLIFHRLEEFL 480
           GA  V VE SEPQ S+DLCFRVSVFQQIPGTLLVKGSLHH+S+PS+G FS IFHRLEEFL
Sbjct: 421 GASGVAVEMSEPQPSADLCFRVSVFQQIPGTLLVKGSLHHKSTPSSGSFSQIFHRLEEFL 480

Query: 481 KAESCKEK 488
           KAESCKEK
Sbjct: 481 KAESCKEK 488

BLAST of Lag0001453 vs. ExPASy Swiss-Prot
Match: Q554C9 (U6 small nuclear RNA (adenine-(43)-N(6))-methyltransferase OS=Dictyostelium discoideum OX=44689 GN=DDB_G0275203 PE=3 SV=1)

HSP 1 Score: 228.0 bits (580), Expect = 2.5e-58
Identity = 138/353 (39.09%), Postives = 198/353 (56.09%), Query Frame = 0

Query: 2   TRKKRKTREPRTIHPRNKYAENPPDFSLLASLYPSFQPFVF--LDPRPRIDWTDFNATRE 61
           T+KKRK R   ++H  N Y  NPP+F LLAS YP+F  ++    +    IDW D NAT+E
Sbjct: 31  TKKKRK-RSNDSMHINNFYRYNPPNFKLLASKYPTFDKYIINKTEKIYNIDWKDSNATKE 90

Query: 62  LTRVLLLHDHALHWWIPDGQLCPTVPNRSNYIHWLEDLLSS--DIIAKANSDRGRVRGFD 121
           LTRVLL HD  L   +PD  LCPT+  R NY++W+ D L +   I+   ++D   ++G D
Sbjct: 91  LTRVLLDHDFGLRIELPDNYLCPTLTLRINYLYWISDQLKNLKIILNDNDNDNKIIKGID 150

Query: 122 IGTGANCIYPLLGASLL-GWSFVGTDVTDVALEWAERNVKSNPHVSELIEIRKVDSTLDG 181
           IGTG +CI+PLLGA L   WSF+G D+ D  LE+A+ N+  N                  
Sbjct: 151 IGTGTSCIFPLLGAKLFNNWSFIGIDIDDKVLEYAQNNITIN------------------ 210

Query: 182 LTTELHNGVAVESESKNLNDIDGKEVRLPLPSSSLKETVYLEKRYHGPPILLGVVKDGEE 241
               L++ + +    KN +DI  K +     S++   +    +  H          D EE
Sbjct: 211 ---SLNSKITLFKNEKN-SDILLKLLNYKEGSNTFNSSNDDHQDNHDDD------DDDEE 270

Query: 242 F--DFCMCNPPFFESMDE----AGLNPKTSCGGTPQEMVCPGGERAFISRMIEDSTVLKQ 301
           +  DFC+CNPPFF+ ++E       NPK++C G+  EMV  GGE  F+ R+I++S  LK 
Sbjct: 271 YFADFCLCNPPFFKDLNENNNNKNNNPKSNCTGSVNEMVTDGGEFEFVKRIIKESFQLKC 330

Query: 302 TFRWYTSMIGKKSNLSFLIS---KLWKVGVTVVKTTEFVQGQTCRWGLAWSFM 341
             R+YT+MIG+K NL+ LI+   K + +    ++TTE VQG T RW L+W F+
Sbjct: 331 KIRFYTTMIGRKVNLNPLINILIKQYYLPKNQIQTTELVQGNTSRWVLSWYFL 354

BLAST of Lag0001453 vs. ExPASy Swiss-Prot
Match: Q6DC64 (RNA N6-adenosine-methyltransferase mettl16 OS=Danio rerio OX=7955 GN=mettl16 PE=2 SV=1)

HSP 1 Score: 220.7 bits (561), Expect = 4.0e-56
Identity = 133/339 (39.23%), Postives = 180/339 (53.10%), Query Frame = 0

Query: 12  RTIHPRNKYAENPPDFSLLASLYPSFQPFV--FLDPRPRIDWTDFNATRELTRVLLLHDH 71
           +++HPRN+Y + PPDF  LAS YP FQ  V   L  R  +++ D  A R LT  LL  D 
Sbjct: 5   KSMHPRNRYKDKPPDFVYLASKYPEFQKHVQTTLTGRVTLNFKDPEAVRALTCTLLKEDF 64

Query: 72  ALHWWIPDGQLCPTVPNRSNYIHWLEDLLSSDIIAKANSDRGRVRGFDIGTGANCIYPLL 131
            L   IP  +L PTVP R NYIHW+EDL+      + N      RG DIGTGA+CIYPLL
Sbjct: 65  GLTIEIPLERLIPTVPLRLNYIHWVEDLIG----GQGNPQ----RGIDIGTGASCIYPLL 124

Query: 132 GASLLGWSFVGTDVTDVALEWAERNVKSNPHVSELIEIRKVDSTLDGLTTELHNGVAVES 191
           GA++ GW F+ T+V D+   +A++NV+ N H++ELI++ KV                   
Sbjct: 125 GATMNGWFFLATEVDDICFNYAKKNVEQN-HLAELIKVVKV------------------- 184

Query: 192 ESKNLNDIDGKEVRLPLPSSSLKETVYLEKRYHGPPILLGVVKDGEEFDFCMCNPPFFES 251
                            P  +L              ++  + ++   +DFCMCNPPFF +
Sbjct: 185 -----------------PQKTL--------------LMDALKEESIVYDFCMCNPPFFAN 244

Query: 252 MDEA-GLNPKTSCGGTPQ--------EMVCPGGERAFISRMIEDSTVLKQTFRWYTSMIG 311
             EA G+N + S    P         E++  GGE  F+ R+I DS  LK+  RWY+ M+G
Sbjct: 245 QLEAKGVNSRNSRRPPPSSINTGGVTEIMAEGGELEFVKRVIHDSLQLKKRLRWYSCMLG 284

Query: 312 KKSNLSFLISKLWKVGVTVVKTTEFVQGQTCRWGLAWSF 340
           KK +L+ L  +L K GV  V  TEF QG+T RW LAWSF
Sbjct: 305 KKCSLAPLKDELRKQGVAKVTHTEFCQGRTMRWALAWSF 284

BLAST of Lag0001453 vs. ExPASy Swiss-Prot
Match: Q6GR37 (RNA N6-adenosine-methyltransferase mettl16 OS=Xenopus laevis OX=8355 GN=mettl16 PE=2 SV=1)

HSP 1 Score: 216.5 bits (550), Expect = 7.5e-55
Identity = 139/360 (38.61%), Postives = 192/360 (53.33%), Query Frame = 0

Query: 12  RTIHPRNKYAENPPDFSLLASLYPSFQPF--VFLDPRPRIDWTDFNATRELTRVLLLHDH 71
           +++HPRN+Y + PPDF+ LAS YP F+    V L  R  +++ D  A R LT  LL  D 
Sbjct: 5   KSMHPRNRYKDKPPDFAYLASKYPEFKQHVNVNLAGRVSLNFKDPCAVRALTCTLLKEDF 64

Query: 72  ALHWWIPDGQLCPTVPNRSNYIHWLEDLLSSDIIAKANSDRGRV-RGFDIGTGANCIYPL 131
            L   IP  +L PTVP R NYIHW+EDL++       +SD+  + RG DIGTGA+CIYPL
Sbjct: 65  GLTIDIPLERLIPTVPLRLNYIHWVEDLINYH-----DSDKTALRRGIDIGTGASCIYPL 124

Query: 132 LGASLLGWSFVGTDVTDVALEWAERNVKSNPHVSELIEIRKVDSTLDGLTTELHNGVAVE 191
           LGA+L GW F+ T+V D+   +A++NV+ N ++S+LI++ KV                  
Sbjct: 125 LGATLNGWYFLATEVDDICYNYAKKNVEQN-NLSDLIKVVKV------------------ 184

Query: 192 SESKNLNDIDGKEVRLPLPSSSLKETVYLEKRYHGPPILLGVVKDGEE--FDFCMCNPPF 251
                             P  +L               L+  +K+  E  +DFCMCNPPF
Sbjct: 185 ------------------PQKTL---------------LMDALKEESEIIYDFCMCNPPF 244

Query: 252 FESMDEA-GLN--------PKTSCGGTPQEMVCPGGERAFISRMIEDSTVLKQTFRWYTS 311
           F +  EA G+N        P +   G   E++  GGE  F+ R+I DS  LK+  RWY+ 
Sbjct: 245 FANQLEAQGVNSRNPHRSPPSSVNTGGITEIMAEGGELEFVKRIIHDSLKLKKRLRWYSC 304

Query: 312 MIGKKSNLSFLISKLWKVGVTVVKTTEFVQGQTCRWGLAWSFMPAARKIISPHVTNKSVL 358
           M+GKK +L+ L  +L   GV  V  TEF QG+T RW LAWSF     ++I P+   K  L
Sbjct: 305 MLGKKCSLAPLKEELRLQGVPKVAHTEFYQGRTMRWALAWSFY---EEVIIPNPPKKRKL 304

BLAST of Lag0001453 vs. ExPASy Swiss-Prot
Match: Q9CQG2 (RNA N6-adenosine-methyltransferase METTL16 OS=Mus musculus OX=10090 GN=Mettl16 PE=1 SV=1)

HSP 1 Score: 216.1 bits (549), Expect = 9.9e-55
Identity = 142/381 (37.27%), Postives = 201/381 (52.76%), Query Frame = 0

Query: 12  RTIHPRNKYAENPPDFSLLASLYPSFQPF--VFLDPRPRIDWTDFNATRELTRVLLLHDH 71
           +++H RN+Y + PPDF+ LAS YP F+    + L+ R  +++ D  A R LT  LL  D 
Sbjct: 5   KSMHARNRYKDKPPDFAYLASKYPDFKQHIQINLNGRVSLNFKDPEAVRALTCTLLREDF 64

Query: 72  ALHWWIPDGQLCPTVPNRSNYIHWLEDLLSSDIIAKANSDRGRV-RGFDIGTGANCIYPL 131
            L   IP  +L PTVP R NYIHW+EDL     I   +SD+  + RG DIGTGA+CIYPL
Sbjct: 65  GLSIDIPLERLIPTVPLRLNYIHWVEDL-----IGHQDSDKTTLRRGIDIGTGASCIYPL 124

Query: 132 LGASLLGWSFVGTDVTDVALEWAERNVKSNPHVSELIEIRKVDSTLDGLTTELHNGVAVE 191
           LGA+L GW F+ T+V D+   +A++NV+ N ++S+LI++ KV                  
Sbjct: 125 LGATLNGWYFLATEVDDMCFNYAKKNVEQN-NLSDLIKVVKV------------------ 184

Query: 192 SESKNLNDIDGKEVRLPLPSSSLKETVYLEKRYHGPPILLGVVKDGEE--FDFCMCNPPF 251
                             P  +L               L+  +K+  E  +DFCMCNPPF
Sbjct: 185 ------------------PQKTL---------------LMDALKEESEIVYDFCMCNPPF 244

Query: 252 FESMDEA-GLNPKTSCGGTPQ--------EMVCPGGERAFISRMIEDSTVLKQTFRWYTS 311
           F +  EA G+N + S    P         E++  GGE  F+ R+I DS  LK+  RWY+ 
Sbjct: 245 FANQLEAKGVNSRNSRRPPPSSVNTGGITEIMAEGGELEFVKRIIHDSLQLKKRLRWYSC 304

Query: 312 MIGKKSNLSFLISKLWKVGVTVVKTTEFVQGQTCRWGLAWSF-----MPA----ARKIIS 370
           M+GKK +L+ L  +L   GV  V  TEF QG+T RW LAWSF     +P+     RK+  
Sbjct: 305 MLGKKCSLAPLKEELRIQGVPKVTFTEFCQGRTMRWALAWSFYDDVTVPSPPSKRRKLEK 325

BLAST of Lag0001453 vs. ExPASy Swiss-Prot
Match: Q86W50 (RNA N6-adenosine-methyltransferase METTL16 OS=Homo sapiens OX=9606 GN=METTL16 PE=1 SV=2)

HSP 1 Score: 215.7 bits (548), Expect = 1.3e-54
Identity = 135/342 (39.47%), Postives = 184/342 (53.80%), Query Frame = 0

Query: 12  RTIHPRNKYAENPPDFSLLASLYPSFQPFV--FLDPRPRIDWTDFNATRELTRVLLLHDH 71
           +++H RN+Y + PPDF+ LAS YP F+  V   L+ R  +++ D  A R LT  LL  D 
Sbjct: 5   KSMHARNRYKDKPPDFAYLASKYPDFKQHVQINLNGRVSLNFKDPEAVRALTCTLLREDF 64

Query: 72  ALHWWIPDGQLCPTVPNRSNYIHWLEDLLSSDIIAKANSDRGRV-RGFDIGTGANCIYPL 131
            L   IP  +L PTVP R NYIHW+EDL     I   +SD+  + RG DIGTGA+CIYPL
Sbjct: 65  GLSIDIPLERLIPTVPLRLNYIHWVEDL-----IGHQDSDKSTLRRGIDIGTGASCIYPL 124

Query: 132 LGASLLGWSFVGTDVTDVALEWAERNVKSNPHVSELIEIRKVDSTLDGLTTELHNGVAVE 191
           LGA+L GW F+ T+V D+   +A++NV+ N ++S+LI++ KV                  
Sbjct: 125 LGATLNGWYFLATEVDDMCFNYAKKNVEQN-NLSDLIKVVKV------------------ 184

Query: 192 SESKNLNDIDGKEVRLPLPSSSLKETVYLEKRYHGPPILLGVVKDGEE--FDFCMCNPPF 251
                             P  +L               L+  +K+  E  +DFCMCNPPF
Sbjct: 185 ------------------PQKTL---------------LMDALKEESEIIYDFCMCNPPF 244

Query: 252 FESMDEA-GLN--------PKTSCGGTPQEMVCPGGERAFISRMIEDSTVLKQTFRWYTS 311
           F +  EA G+N        P +   G   E++  GGE  F+ R+I DS  LK+  RWY+ 
Sbjct: 245 FANQLEAKGVNSRNPRRPPPSSVNTGGITEIMAEGGELEFVKRIIHDSLQLKKRLRWYSC 289

Query: 312 MIGKKSNLSFLISKLWKVGVTVVKTTEFVQGQTCRWGLAWSF 340
           M+GKK +L+ L  +L   GV  V  TEF QG+T RW LAWSF
Sbjct: 305 MLGKKCSLAPLKEELRIQGVPKVTYTEFCQGRTMRWALAWSF 289

BLAST of Lag0001453 vs. ExPASy TrEMBL
Match: A0A6J1I146 (U6 small nuclear RNA (adenine-(43)-N(6))-methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111468473 PE=3 SV=1)

HSP 1 Score: 901.7 bits (2329), Expect = 1.4e-258
Identity = 439/488 (89.96%), Postives = 459/488 (94.06%), Query Frame = 0

Query: 1   MTRKKRKTREPRTIHPRNKYAENPPDFSLLASLYPSFQPFVFLDPRPRIDWTDFNATREL 60
           MTRKKRKTRE  TIHPRNKYAENPPDF+LLASLYPSFQPFVFL+PRPRIDWTDFNATREL
Sbjct: 1   MTRKKRKTREAATIHPRNKYAENPPDFALLASLYPSFQPFVFLEPRPRIDWTDFNATREL 60

Query: 61  TRVLLLHDHALHWWIPDGQLCPTVPNRSNYIHWLEDLLSSDIIAKANSDRG-RVRGFDIG 120
           TRVLLLHDHALHWWIPDGQLCPTVPNRSNYIHWLEDLLSSD++AKAN DRG  VRGFDIG
Sbjct: 61  TRVLLLHDHALHWWIPDGQLCPTVPNRSNYIHWLEDLLSSDVVAKANRDRGSNVRGFDIG 120

Query: 121 TGANCIYPLLGASLLGWSFVGTDVTDVALEWAERNVKSNPHVSELIEIRKVDSTLDGLTT 180
           TGANCIYPLLGASLLGWSFVGTDVTDVALEWAERNVKSNPHVSELIEIRKVD TLDGLTT
Sbjct: 121 TGANCIYPLLGASLLGWSFVGTDVTDVALEWAERNVKSNPHVSELIEIRKVDDTLDGLTT 180

Query: 181 ELHNGVAVESESKNLNDIDGKEVRLPLPSSSLKETVYLEKRYHGPPILLGVVKDGEEFDF 240
           EL + VAV++E K +N +DG+EV    PSSS KET++ EKRYHGPPILLGVVKDGEEFDF
Sbjct: 181 ELRSNVAVDNERKIMNGMDGREVGPSPPSSSSKETIHSEKRYHGPPILLGVVKDGEEFDF 240

Query: 241 CMCNPPFFESMDEAGLNPKTSCGGTPQEMVCPGGERAFISRMIEDSTVLKQTFRWYTSMI 300
           CMCNPPFFESMDEAGLNPKT+CGGTPQEMVCPGGERAFISRMIEDS VLKQTFRWYTSMI
Sbjct: 241 CMCNPPFFESMDEAGLNPKTACGGTPQEMVCPGGERAFISRMIEDSIVLKQTFRWYTSMI 300

Query: 301 GKKSNLSFLISKLWKVGVTVVKTTEFVQGQTCRWGLAWSFMPAARKIISPHVTNKSVLSF 360
           GKKSNLSFLISKLWKVGVTVVKTTEFVQGQTCRWGLAWSFMPA RKIISPHVTNKSV SF
Sbjct: 301 GKKSNLSFLISKLWKVGVTVVKTTEFVQGQTCRWGLAWSFMPAGRKIISPHVTNKSVSSF 360

Query: 361 MLEGLLPQVSAMSILQSVELFLSTGGASCKLNTSSFTVDITASEDHCQAILKNGAFEVDK 420
           MLEGLLPQVSAMSILQSVE FLS GGASCKLNTSSFTVD+TAS DHC+AIL+NGA+EVDK
Sbjct: 361 MLEGLLPQVSAMSILQSVERFLSAGGASCKLNTSSFTVDVTASNDHCRAILENGAYEVDK 420

Query: 421 GAGCVTVEKSEPQLSSDLCFRVSVFQQIPGTLLVKGSLHHRSSPSAGLFSLIFHRLEEFL 480
           GA  V VEKSEPQ S+DLCFRVSVFQQIPGTLLVKGSLHH+SSPS+G FS IFHRLEEFL
Sbjct: 421 GASGVPVEKSEPQPSTDLCFRVSVFQQIPGTLLVKGSLHHKSSPSSGSFSQIFHRLEEFL 480

Query: 481 KAESCKEK 488
           KAESCKEK
Sbjct: 481 KAESCKEK 488

BLAST of Lag0001453 vs. ExPASy TrEMBL
Match: A0A6J1GKM3 (U6 small nuclear RNA (adenine-(43)-N(6))-methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111455141 PE=3 SV=1)

HSP 1 Score: 897.5 bits (2318), Expect = 2.7e-257
Identity = 437/488 (89.55%), Postives = 458/488 (93.85%), Query Frame = 0

Query: 1   MTRKKRKTREPRTIHPRNKYAENPPDFSLLASLYPSFQPFVFLDPRPRIDWTDFNATREL 60
           MTRKKRKTRE  TIHPRNKYAENPPDF+LLASLYPSFQPFVFL+PRPRIDWTDFNATREL
Sbjct: 1   MTRKKRKTREAATIHPRNKYAENPPDFALLASLYPSFQPFVFLEPRPRIDWTDFNATREL 60

Query: 61  TRVLLLHDHALHWWIPDGQLCPTVPNRSNYIHWLEDLLSSDIIAKANSDRG-RVRGFDIG 120
           TRVLLLHDHALHWWIPDGQLCPTVPNRSNYIHWLEDLLSSD++AKANSDRG  VRGFDIG
Sbjct: 61  TRVLLLHDHALHWWIPDGQLCPTVPNRSNYIHWLEDLLSSDVVAKANSDRGSNVRGFDIG 120

Query: 121 TGANCIYPLLGASLLGWSFVGTDVTDVALEWAERNVKSNPHVSELIEIRKVDSTLDGLTT 180
           TGANCIYPLLGASLLGWSFVGTDVT+VALEWAERNVKSNPHVSELIEIRKVD TLDGLT 
Sbjct: 121 TGANCIYPLLGASLLGWSFVGTDVTNVALEWAERNVKSNPHVSELIEIRKVDDTLDGLTI 180

Query: 181 ELHNGVAVESESKNLNDIDGKEVRLPLPSSSLKETVYLEKRYHGPPILLGVVKDGEEFDF 240
           EL + VAV++E K +N +DG+EV    PSSS KET++ EKRYHGPPILLGVVKDGEEFDF
Sbjct: 181 ELRSNVAVDNECKIMNGMDGREVGPSPPSSSSKETIHSEKRYHGPPILLGVVKDGEEFDF 240

Query: 241 CMCNPPFFESMDEAGLNPKTSCGGTPQEMVCPGGERAFISRMIEDSTVLKQTFRWYTSMI 300
           CMCNPPFFESMDEAGLNPKT+CGGTPQEMVCPGGERAFISRMIEDS VLKQTFRWYTSMI
Sbjct: 241 CMCNPPFFESMDEAGLNPKTACGGTPQEMVCPGGERAFISRMIEDSIVLKQTFRWYTSMI 300

Query: 301 GKKSNLSFLISKLWKVGVTVVKTTEFVQGQTCRWGLAWSFMPAARKIISPHVTNKSVLSF 360
           GKKSNLSFLISKLWKVGVTVVKTTEFVQGQTCRWGLAWSFMPA RKIISPHVTNKSV SF
Sbjct: 301 GKKSNLSFLISKLWKVGVTVVKTTEFVQGQTCRWGLAWSFMPAGRKIISPHVTNKSVSSF 360

Query: 361 MLEGLLPQVSAMSILQSVELFLSTGGASCKLNTSSFTVDITASEDHCQAILKNGAFEVDK 420
           MLEGLLPQVSA SILQSVE FLS GGASCKLNTSSFTVD+TAS DHC+AIL+NGA+EVDK
Sbjct: 361 MLEGLLPQVSATSILQSVERFLSVGGASCKLNTSSFTVDVTASNDHCRAILENGAYEVDK 420

Query: 421 GAGCVTVEKSEPQLSSDLCFRVSVFQQIPGTLLVKGSLHHRSSPSAGLFSLIFHRLEEFL 480
           GA  V VEKSEPQ S+DLCFRVSVFQQIPGTLLVKGSLHH+SSPS+G FS IFHRLEEFL
Sbjct: 421 GASGVAVEKSEPQPSADLCFRVSVFQQIPGTLLVKGSLHHKSSPSSGSFSQIFHRLEEFL 480

Query: 481 KAESCKEK 488
           KAESCKEK
Sbjct: 481 KAESCKEK 488

BLAST of Lag0001453 vs. ExPASy TrEMBL
Match: A0A6J1C2I0 (U6 small nuclear RNA (adenine-(43)-N(6))-methyltransferase OS=Momordica charantia OX=3673 GN=LOC111007785 PE=3 SV=1)

HSP 1 Score: 894.8 bits (2311), Expect = 1.8e-256
Identity = 437/487 (89.73%), Postives = 459/487 (94.25%), Query Frame = 0

Query: 1   MTRKKRKTREPRTIHPRNKYAENPPDFSLLASLYPSFQPFVFLDPRPRIDWTDFNATREL 60
           MTR KRKTRE  TIHPRNKYAENPPDF+LLASLYPSFQPFVFL PRPRI+WTDFNATREL
Sbjct: 1   MTRNKRKTREALTIHPRNKYAENPPDFALLASLYPSFQPFVFLHPRPRIEWTDFNATREL 60

Query: 61  TRVLLLHDHALHWWIPDGQLCPTVPNRSNYIHWLEDLLSSDIIAKANSDRGRVRGFDIGT 120
           TRVLLLHDH L+WWIPDGQLCPTVPNRSNYIHWLEDLLSSDIIA  NSDRG VRGFDIGT
Sbjct: 61  TRVLLLHDHGLNWWIPDGQLCPTVPNRSNYIHWLEDLLSSDIIANTNSDRGCVRGFDIGT 120

Query: 121 GANCIYPLLGASLLGWSFVGTDVTDVALEWAERNVKSNPHVSELIEIRKVDSTLDGLTTE 180
           GANCIYPLLGASLLGWSFVGTDVTDVALEWAE NVKSNPHVSEL+EIRKVDST + L+TE
Sbjct: 121 GANCIYPLLGASLLGWSFVGTDVTDVALEWAETNVKSNPHVSELVEIRKVDSTSNTLSTE 180

Query: 181 LHNGVAVESESKNLNDIDGKEVRLPLPSSSLKETVYLEKRYHGPPILLGVVKDGEEFDFC 240
           LH+GVAV SESKN +D+DG+ V  P PSSSL ETVYLEKRYHGPPILL VVKDGE+FDFC
Sbjct: 181 LHDGVAVNSESKNSDDMDGRAVGPPSPSSSLTETVYLEKRYHGPPILLDVVKDGEKFDFC 240

Query: 241 MCNPPFFESMDEAGLNPKTSCGGTPQEMVCPGGERAFISRMIEDSTVLKQTFRWYTSMIG 300
           MCNPPFF SMDEAGLNPKTSCGGT QEMVCPGGERAFISRMIEDSTVLKQTFRWYTSMIG
Sbjct: 241 MCNPPFFASMDEAGLNPKTSCGGTLQEMVCPGGERAFISRMIEDSTVLKQTFRWYTSMIG 300

Query: 301 KKSNLSFLISKLWKVGVTVVKTTEFVQGQTCRWGLAWSFMPAARKIISPHVTNKSVLSFM 360
           KKSNLSFLISKLWKVGVTVVKTTEFVQGQT RWGLAWSFMPAARKIIS HVTNKSVLSFM
Sbjct: 301 KKSNLSFLISKLWKVGVTVVKTTEFVQGQTSRWGLAWSFMPAARKIISTHVTNKSVLSFM 360

Query: 361 LEGLLPQVSAMSILQSVELFLSTGGASCKLNTSSFTVDITASEDHCQAILKNGAFEVDKG 420
           LEGLLP+V+AMSILQS+E FLSTGGASCKLNTSSFTVDITASEDHCQAILKNGA++VDKG
Sbjct: 361 LEGLLPRVNAMSILQSIEHFLSTGGASCKLNTSSFTVDITASEDHCQAILKNGAYQVDKG 420

Query: 421 AGCVTVEKSEPQLSSDLCFRVSVFQQIPGTLLVKGSLHHRSSPSAGLFSLIFHRLEEFLK 480
           AGCVTV++SE Q S++LCFRVSVFQQIPGTLLVKGSLHH SS SAG+FSLIFHRLEEFLK
Sbjct: 421 AGCVTVKESESQPSNNLCFRVSVFQQIPGTLLVKGSLHHSSSASAGMFSLIFHRLEEFLK 480

Query: 481 AESCKEK 488
           AESC+EK
Sbjct: 481 AESCREK 487

BLAST of Lag0001453 vs. ExPASy TrEMBL
Match: A0A1S3BIM5 (U6 small nuclear RNA (adenine-(43)-N(6))-methyltransferase OS=Cucumis melo OX=3656 GN=LOC103490299 PE=3 SV=1)

HSP 1 Score: 888.3 bits (2294), Expect = 1.6e-254
Identity = 432/485 (89.07%), Postives = 453/485 (93.40%), Query Frame = 0

Query: 1   MTRKKRKTREPRTIHPRNKYAENPPDFSLLASLYPSFQPFVFLDPRPRIDWTDFNATREL 60
           MTRKKRKTRE  TIHPRNKYAENPPDF+LLASLYPSFQPFVFL PRPRIDWTDFNATREL
Sbjct: 1   MTRKKRKTREAATIHPRNKYAENPPDFALLASLYPSFQPFVFLHPRPRIDWTDFNATREL 60

Query: 61  TRVLLLHDHALHWWIPDGQLCPTVPNRSNYIHWLEDLLSSDIIAKANSDRGRVRGFDIGT 120
           TRVLLLHDHALHWWIPDGQLCPTVPNRSNYIHWLEDLL+SDI+AK NSD GRVRGFDIGT
Sbjct: 61  TRVLLLHDHALHWWIPDGQLCPTVPNRSNYIHWLEDLLASDIVAKKNSDCGRVRGFDIGT 120

Query: 121 GANCIYPLLGASLLGWSFVGTDVTDVALEWAERNVKSNPHVSELIEIRKVDSTLDGLTTE 180
           GANCIYPLLGASLLGWSFVGTDVTDVALEWAE+NVKSNPHVSELIEIRKVD T D L+TE
Sbjct: 121 GANCIYPLLGASLLGWSFVGTDVTDVALEWAEKNVKSNPHVSELIEIRKVDDTSDNLSTE 180

Query: 181 LHNGVAVESESKNLNDIDGKEVRLPLPSSSLKETVYLEKRYHGPPILLGVVKDGEEFDFC 240
           LH+ VAV+SE K  N++ G+EV  PLPS  LKE V+LEK+YHGPPILLGVVKDGEEFDFC
Sbjct: 181 LHDSVAVDSECKIFNEMGGREVGPPLPSFLLKEAVHLEKQYHGPPILLGVVKDGEEFDFC 240

Query: 241 MCNPPFFESMDEAGLNPKTSCGGTPQEMVCPGGERAFISRMIEDSTVLKQTFRWYTSMIG 300
           MCNPPFFESMDEAGLNPKTSCGGT QEMVCPGGERAFISRMIEDS VLKQTFRWYTSMIG
Sbjct: 241 MCNPPFFESMDEAGLNPKTSCGGTSQEMVCPGGERAFISRMIEDSIVLKQTFRWYTSMIG 300

Query: 301 KKSNLSFLISKLWKVGVTVVKTTEFVQGQTCRWGLAWSFMPAARKIISPHVTNKSVLSFM 360
           KKSNLSFLISKLWKVGVTVVKTTEFVQGQTCRWGLAWSFM  ARKIISPHVTNKS+LSFM
Sbjct: 301 KKSNLSFLISKLWKVGVTVVKTTEFVQGQTCRWGLAWSFMSTARKIISPHVTNKSILSFM 360

Query: 361 LEGLLPQVSAMSILQSVELFLSTGGASCKLNTSSFTVDITASEDHCQAILKNGAFEVDKG 420
           LEGLLPQVSAMSILQSVE FLSTGGASCKLNTSSFTVDITAS DHC+AI++NGA + DK 
Sbjct: 361 LEGLLPQVSAMSILQSVERFLSTGGASCKLNTSSFTVDITASVDHCRAIIENGACQEDKV 420

Query: 421 AGCVTVEKSEPQLSSDLCFRVSVFQQIPGTLLVKGSLHHRSSPSAGLFSLIFHRLEEFLK 480
           A CV +EKSE QLS+DLCFRVSVFQQIPGTLLVKGSLH +SSPSAGLFSLIFHRLEEFLK
Sbjct: 421 ASCVALEKSETQLSTDLCFRVSVFQQIPGTLLVKGSLHQKSSPSAGLFSLIFHRLEEFLK 480

Query: 481 AESCK 486
           A+ CK
Sbjct: 481 ADFCK 485

BLAST of Lag0001453 vs. ExPASy TrEMBL
Match: A0A0A0K5X1 (U6 small nuclear RNA (adenine-(43)-N(6))-methyltransferase OS=Cucumis sativus OX=3659 GN=Csa_7G009800 PE=3 SV=1)

HSP 1 Score: 880.9 bits (2275), Expect = 2.6e-252
Identity = 428/485 (88.25%), Postives = 448/485 (92.37%), Query Frame = 0

Query: 1   MTRKKRKTREPRTIHPRNKYAENPPDFSLLASLYPSFQPFVFLDPRPRIDWTDFNATREL 60
           MTRKKRKTRE  TIHPRNKYAENPPDF+LLASLYPSFQPFV L PRPRIDWTDFNATREL
Sbjct: 1   MTRKKRKTREAATIHPRNKYAENPPDFALLASLYPSFQPFVSLHPRPRIDWTDFNATREL 60

Query: 61  TRVLLLHDHALHWWIPDGQLCPTVPNRSNYIHWLEDLLSSDIIAKANSDRGRVRGFDIGT 120
           TRVLLLHDHALHWWIPDGQLCPTVPNRSNYIHWLEDLLSSD++AK NSD GRVRGFDIGT
Sbjct: 61  TRVLLLHDHALHWWIPDGQLCPTVPNRSNYIHWLEDLLSSDVVAKKNSDCGRVRGFDIGT 120

Query: 121 GANCIYPLLGASLLGWSFVGTDVTDVALEWAERNVKSNPHVSELIEIRKVDSTLDGLTTE 180
           GANCIYPLLGASLLGWSFVGTDVTDVALEWAERNV SNPH+SELIEIRKVD T D L+TE
Sbjct: 121 GANCIYPLLGASLLGWSFVGTDVTDVALEWAERNVTSNPHISELIEIRKVDDTSDDLSTE 180

Query: 181 LHNGVAVESESKNLNDIDGKEVRLPLPSSSLKETVYLEKRYHGPPILLGVVKDGEEFDFC 240
           LH+ VAV+SE K  N++DG+E+  PLPS   KE ++LE +YHGPPILLGVVKDGE FDFC
Sbjct: 181 LHDSVAVDSECKIFNEMDGREIGPPLPSFLSKEAIHLETQYHGPPILLGVVKDGERFDFC 240

Query: 241 MCNPPFFESMDEAGLNPKTSCGGTPQEMVCPGGERAFISRMIEDSTVLKQTFRWYTSMIG 300
           MCNPPFFESMDEAGLNPKTSCGGTPQEMVCPGGERAFISRMIEDS VLKQTFRWYTSMIG
Sbjct: 241 MCNPPFFESMDEAGLNPKTSCGGTPQEMVCPGGERAFISRMIEDSIVLKQTFRWYTSMIG 300

Query: 301 KKSNLSFLISKLWKVGVTVVKTTEFVQGQTCRWGLAWSFMPAARKIISPHVTNKSVLSFM 360
           KKSNLSFLISKLWKVGVTVVKTTEFVQGQTCRWGLAWSFM  ARKIISPHVTNKS+LSFM
Sbjct: 301 KKSNLSFLISKLWKVGVTVVKTTEFVQGQTCRWGLAWSFMSTARKIISPHVTNKSILSFM 360

Query: 361 LEGLLPQVSAMSILQSVELFLSTGGASCKLNTSSFTVDITASEDHCQAILKNGAFEVDKG 420
           LEGLL QVSAMSILQSVE FLSTGGASCKLN SSFTVDITASEDHCQAIL+NGA E  K 
Sbjct: 361 LEGLLSQVSAMSILQSVERFLSTGGASCKLNASSFTVDITASEDHCQAILENGACEEVKD 420

Query: 421 AGCVTVEKSEPQLSSDLCFRVSVFQQIPGTLLVKGSLHHRSSPSAGLFSLIFHRLEEFLK 480
           A CV +EKSE QLS+DLCFRVSVFQQIPGTLLVKGSLH +S+PSAGLFSLIFHRLEEFLK
Sbjct: 421 ASCVALEKSETQLSTDLCFRVSVFQQIPGTLLVKGSLHQKSNPSAGLFSLIFHRLEEFLK 480

Query: 481 AESCK 486
           AE CK
Sbjct: 481 AEFCK 485

BLAST of Lag0001453 vs. TAIR 10
Match: AT2G21070.1 (methyltransferases )

HSP 1 Score: 597.0 bits (1538), Expect = 1.5e-170
Identity = 304/489 (62.17%), Postives = 368/489 (75.26%), Query Frame = 0

Query: 4   KKRKTREP-RTIHPRNKYAENPPDFSLLASLYPSFQPFVFLD-PRPRIDWTDFNATRELT 63
           KKR   EP  T  PR+KY++NPPDF+ LASLYPSF+PFVF    R RIDWTD+NATRELT
Sbjct: 5   KKRARSEPGSTKQPRSKYSDNPPDFASLASLYPSFKPFVFFSGSRARIDWTDYNATRELT 64

Query: 64  RVLLLHDHALHWWIPDGQLCPTVPNRSNYIHWLEDLLSSDIIAKANSDRGRVRGFDIGTG 123
           RVLLLHDH ++WWIPDGQLCPTVPNRSNYIHW+ DLLSS II     D  +V+GFDIGTG
Sbjct: 65  RVLLLHDHGVNWWIPDGQLCPTVPNRSNYIHWINDLLSSGIIQSLGGDGSKVKGFDIGTG 124

Query: 124 ANCIYPLLGASLLGWSFVGTDVTDVALEWAERNVKSNPHVSELIEIRKVDSTLDGLTTEL 183
           ANCIYPLLGASL GWSFVG+D T VALEWAE+NV+SNPH S+LIEIR  DS +    + +
Sbjct: 125 ANCIYPLLGASLFGWSFVGSDFTVVALEWAEKNVQSNPHFSDLIEIR--DSKVPPQCSSV 184

Query: 184 HNGVAVESESKNLNDIDGKEVRLPLPSSSLKETVYLEKRYHGPPILLGVVKDGEEFDFCM 243
                VE+  +     +  E+     S+++K   +  K +  P +LLGVVK+ E FDFCM
Sbjct: 185 PE---VENTEREKTIQEEAEI-----SATVKSDYHDNKSFIEPAVLLGVVKENETFDFCM 244

Query: 244 CNPPFFESMDEAGLNPKTSCGGTPQEMVCPGGERAFISRMIEDSTVLKQTFRWYTSMIGK 303
            NPPFFE+ +EAGLNPKTSCGGTP+EMVC GGE+AF+SR+I+DS VL+Q FRWYTSM+GK
Sbjct: 245 SNPPFFETFEEAGLNPKTSCGGTPEEMVCNGGEQAFVSRIIKDSAVLRQRFRWYTSMLGK 304

Query: 304 KSNLSFLISKLWKVGVTVVKTTEFVQGQTCRWGLAWSFMPAARKIISPHVTNKSVLSFML 363
           K+NL  LISKLW+VGVT+VKTTEFVQGQT RWGLAWSFMP ARKII+P V  KSVLSFML
Sbjct: 305 KANLKLLISKLWEVGVTIVKTTEFVQGQTSRWGLAWSFMPIARKIIAPPVVKKSVLSFML 364

Query: 364 EGLLPQVSAMSILQSVELFLSTGGASCKLNTSSFTVDITASEDHCQAILKNGAFEVDK-- 423
           E +  Q SA+ +LQSVE F  + GASCKLN+S+F+VDI AS D C  I KN   +VD   
Sbjct: 365 ECIKRQYSAVDVLQSVEEFFKSCGASCKLNSSTFSVDIVASNDQCNTISKNDIADVDSVR 424

Query: 424 --GAGCVTVEKSEPQLSS-DLCFRVSVFQQIPGTLLVKGSLHHRSSPSAGLFSLIFHRLE 483
             G    +++ S  Q+   +L FR+ VFQQ+PGTLL+KGSL  + SP +GLFS +F  LE
Sbjct: 425 SHGYEKQSLDGSSLQVPEYNLSFRILVFQQMPGTLLIKGSLQQKDSPLSGLFSRVFGSLE 483

Query: 484 EFLKAESCK 486
           E LK++ C+
Sbjct: 485 ESLKSKFCR 483

BLAST of Lag0001453 vs. TAIR 10
Match: AT2G21070.3 (methyltransferases )

HSP 1 Score: 597.0 bits (1538), Expect = 1.5e-170
Identity = 304/489 (62.17%), Postives = 368/489 (75.26%), Query Frame = 0

Query: 4   KKRKTREP-RTIHPRNKYAENPPDFSLLASLYPSFQPFVFLD-PRPRIDWTDFNATRELT 63
           KKR   EP  T  PR+KY++NPPDF+ LASLYPSF+PFVF    R RIDWTD+NATRELT
Sbjct: 5   KKRARSEPGSTKQPRSKYSDNPPDFASLASLYPSFKPFVFFSGSRARIDWTDYNATRELT 64

Query: 64  RVLLLHDHALHWWIPDGQLCPTVPNRSNYIHWLEDLLSSDIIAKANSDRGRVRGFDIGTG 123
           RVLLLHDH ++WWIPDGQLCPTVPNRSNYIHW+ DLLSS II     D  +V+GFDIGTG
Sbjct: 65  RVLLLHDHGVNWWIPDGQLCPTVPNRSNYIHWINDLLSSGIIQSLGGDGSKVKGFDIGTG 124

Query: 124 ANCIYPLLGASLLGWSFVGTDVTDVALEWAERNVKSNPHVSELIEIRKVDSTLDGLTTEL 183
           ANCIYPLLGASL GWSFVG+D T VALEWAE+NV+SNPH S+LIEIR  DS +    + +
Sbjct: 125 ANCIYPLLGASLFGWSFVGSDFTVVALEWAEKNVQSNPHFSDLIEIR--DSKVPPQCSSV 184

Query: 184 HNGVAVESESKNLNDIDGKEVRLPLPSSSLKETVYLEKRYHGPPILLGVVKDGEEFDFCM 243
                VE+  +     +  E+     S+++K   +  K +  P +LLGVVK+ E FDFCM
Sbjct: 185 PE---VENTEREKTIQEEAEI-----SATVKSDYHDNKSFIEPAVLLGVVKENETFDFCM 244

Query: 244 CNPPFFESMDEAGLNPKTSCGGTPQEMVCPGGERAFISRMIEDSTVLKQTFRWYTSMIGK 303
            NPPFFE+ +EAGLNPKTSCGGTP+EMVC GGE+AF+SR+I+DS VL+Q FRWYTSM+GK
Sbjct: 245 SNPPFFETFEEAGLNPKTSCGGTPEEMVCNGGEQAFVSRIIKDSAVLRQRFRWYTSMLGK 304

Query: 304 KSNLSFLISKLWKVGVTVVKTTEFVQGQTCRWGLAWSFMPAARKIISPHVTNKSVLSFML 363
           K+NL  LISKLW+VGVT+VKTTEFVQGQT RWGLAWSFMP ARKII+P V  KSVLSFML
Sbjct: 305 KANLKLLISKLWEVGVTIVKTTEFVQGQTSRWGLAWSFMPIARKIIAPPVVKKSVLSFML 364

Query: 364 EGLLPQVSAMSILQSVELFLSTGGASCKLNTSSFTVDITASEDHCQAILKNGAFEVDK-- 423
           E +  Q SA+ +LQSVE F  + GASCKLN+S+F+VDI AS D C  I KN   +VD   
Sbjct: 365 ECIKRQYSAVDVLQSVEEFFKSCGASCKLNSSTFSVDIVASNDQCNTISKNDIADVDSVR 424

Query: 424 --GAGCVTVEKSEPQLSS-DLCFRVSVFQQIPGTLLVKGSLHHRSSPSAGLFSLIFHRLE 483
             G    +++ S  Q+   +L FR+ VFQQ+PGTLL+KGSL  + SP +GLFS +F  LE
Sbjct: 425 SHGYEKQSLDGSSLQVPEYNLSFRILVFQQMPGTLLIKGSLQQKDSPLSGLFSRVFGSLE 483

Query: 484 EFLKAESCK 486
           E LK++ C+
Sbjct: 485 ESLKSKFCR 483

BLAST of Lag0001453 vs. TAIR 10
Match: AT2G21070.2 (methyltransferases )

HSP 1 Score: 505.4 bits (1300), Expect = 5.8e-143
Identity = 251/382 (65.71%), Postives = 297/382 (77.75%), Query Frame = 0

Query: 4   KKRKTREP-RTIHPRNKYAENPPDFSLLASLYPSFQPFVFLD-PRPRIDWTDFNATRELT 63
           KKR   EP  T  PR+KY++NPPDF+ LASLYPSF+PFVF    R RIDWTD+NATRELT
Sbjct: 5   KKRARSEPGSTKQPRSKYSDNPPDFASLASLYPSFKPFVFFSGSRARIDWTDYNATRELT 64

Query: 64  RVLLLHDHALHWWIPDGQLCPTVPNRSNYIHWLEDLLSSDIIAKANSDRGRVRGFDIGTG 123
           RVLLLHDH ++WWIPDGQLCPTVPNRSNYIHW+ DLLSS II     D  +V+GFDIGTG
Sbjct: 65  RVLLLHDHGVNWWIPDGQLCPTVPNRSNYIHWINDLLSSGIIQSLGGDGSKVKGFDIGTG 124

Query: 124 ANCIYPLLGASLLGWSFVGTDVTDVALEWAERNVKSNPHVSELIEIRKVDSTLDGLTTEL 183
           ANCIYPLLGASL GWSFVG+D T VALEWAE+NV+SNPH S+LIEIR  DS +    + +
Sbjct: 125 ANCIYPLLGASLFGWSFVGSDFTVVALEWAEKNVQSNPHFSDLIEIR--DSKVPPQCSSV 184

Query: 184 HNGVAVESESKNLNDIDGKEVRLPLPSSSLKETVYLEKRYHGPPILLGVVKDGEEFDFCM 243
                VE+  +     +  E+     S+++K   +  K +  P +LLGVVK+ E FDFCM
Sbjct: 185 PE---VENTEREKTIQEEAEI-----SATVKSDYHDNKSFIEPAVLLGVVKENETFDFCM 244

Query: 244 CNPPFFESMDEAGLNPKTSCGGTPQEMVCPGGERAFISRMIEDSTVLKQTFRWYTSMIGK 303
            NPPFFE+ +EAGLNPKTSCGGTP+EMVC GGE+AF+SR+I+DS VL+Q FRWYTSM+GK
Sbjct: 245 SNPPFFETFEEAGLNPKTSCGGTPEEMVCNGGEQAFVSRIIKDSAVLRQRFRWYTSMLGK 304

Query: 304 KSNLSFLISKLWKVGVTVVKTTEFVQGQTCRWGLAWSFMPAARKIISPHVTNKSVLSFML 363
           K+NL  LISKLW+VGVT+VKTTEFVQGQT RWGLAWSFMP ARKII+P V  KSVLSFML
Sbjct: 305 KANLKLLISKLWEVGVTIVKTTEFVQGQTSRWGLAWSFMPIARKIIAPPVVKKSVLSFML 364

Query: 364 EGLLPQVSAMSILQSVELFLST 384
           E +  Q SA+ +LQSVE F  +
Sbjct: 365 ECIKRQYSAVDVLQSVEEFFKS 376

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022969493.13.0e-25889.96U6 small nuclear RNA (adenine-(43)-N(6))-methyltransferase [Cucurbita maxima][more]
XP_038886852.13.3e-25790.55U6 small nuclear RNA (adenine-(43)-N(6))-methyltransferase isoform X2 [Benincasa... [more]
KAG6572103.14.3e-25789.55U6 small nuclear RNA (adenine-(43)-N(6))-methyltransferase, partial [Cucurbita a... [more]
XP_022952458.15.6e-25789.55U6 small nuclear RNA (adenine-(43)-N(6))-methyltransferase [Cucurbita moschata][more]
XP_023554274.12.1e-25689.34U6 small nuclear RNA (adenine-(43)-N(6))-methyltransferase [Cucurbita pepo subsp... [more]
Match NameE-valueIdentityDescription
Q554C92.5e-5839.09U6 small nuclear RNA (adenine-(43)-N(6))-methyltransferase OS=Dictyostelium disc... [more]
Q6DC644.0e-5639.23RNA N6-adenosine-methyltransferase mettl16 OS=Danio rerio OX=7955 GN=mettl16 PE=... [more]
Q6GR377.5e-5538.61RNA N6-adenosine-methyltransferase mettl16 OS=Xenopus laevis OX=8355 GN=mettl16 ... [more]
Q9CQG29.9e-5537.27RNA N6-adenosine-methyltransferase METTL16 OS=Mus musculus OX=10090 GN=Mettl16 P... [more]
Q86W501.3e-5439.47RNA N6-adenosine-methyltransferase METTL16 OS=Homo sapiens OX=9606 GN=METTL16 PE... [more]
Match NameE-valueIdentityDescription
A0A6J1I1461.4e-25889.96U6 small nuclear RNA (adenine-(43)-N(6))-methyltransferase OS=Cucurbita maxima O... [more]
A0A6J1GKM32.7e-25789.55U6 small nuclear RNA (adenine-(43)-N(6))-methyltransferase OS=Cucurbita moschata... [more]
A0A6J1C2I01.8e-25689.73U6 small nuclear RNA (adenine-(43)-N(6))-methyltransferase OS=Momordica charanti... [more]
A0A1S3BIM51.6e-25489.07U6 small nuclear RNA (adenine-(43)-N(6))-methyltransferase OS=Cucumis melo OX=36... [more]
A0A0A0K5X12.6e-25288.25U6 small nuclear RNA (adenine-(43)-N(6))-methyltransferase OS=Cucumis sativus OX... [more]
Match NameE-valueIdentityDescription
AT2G21070.11.5e-17062.17methyltransferases [more]
AT2G21070.31.5e-17062.17methyltransferases [more]
AT2G21070.25.8e-14365.71methyltransferases [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 45..340
e-value: 2.4E-85
score: 287.9
NoneNo IPR availablePIRSRPIRSR037350-1PIRSR037350-1coord: 45..174
e-value: 1.3E-43
score: 147.0
coord: 229..345
e-value: 5.1E-42
score: 141.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..21
IPR010286METTL16/RlmF familyPFAMPF05971Methyltransf_10coord: 210..340
e-value: 1.7E-30
score: 106.4
coord: 12..172
e-value: 4.2E-48
score: 164.2
IPR010286METTL16/RlmF familyPANTHERPTHR13393SAM-DEPENDENT METHYLTRANSFERASEcoord: 3..475
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 57..339

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0001453.1Lag0001453.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032259 methylation
molecular_function GO:0008168 methyltransferase activity