Lag0001432 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0001432 vs. NCBI nr
Match: XP_023529731.1 (uncharacterized protein LOC111792465 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 650.6 bits (1677), Expect = 8.4e-183
Identity = 348/384 (90.62%), Postives = 359/384 (93.49%), Query Frame = 0

Query: 1   MDRLLYSPSPSPPSLKFNPNPSLLLPRFARIDSLKLNFAFPMRHGLRPLGFFSFKDEKPS 60
           MDRLLYSPSP+  S K NPNP LLLPRF RIDS KLNF  P RHGLRPLG FS K EKPS
Sbjct: 1   MDRLLYSPSPT--SFKINPNPPLLLPRFRRIDSAKLNFPLPTRHGLRPLGLFSSKTEKPS 60

Query: 61  MSSIRGLSSSNSSSGSICPADSRSGSPKPDLLEHFAGKEKVKKSLFVLSALALILIQPVF 120
           M  I  LSSSNSSS SI  +DSR+GSPKPDLLEHFAGK+KVK+SLFVLSALALILIQPVF
Sbjct: 61  MPLIHCLSSSNSSSDSISTSDSRAGSPKPDLLEHFAGKKKVKQSLFVLSALALILIQPVF 120

Query: 121 APAAFASFQNAAKTGGPAAAAVGRRLIQSELLSSAWTGFFAGCLHTLSGPDHLAALAPLS 180
           APAAFASFQNAAKTGGPAAAAVGRRLIQSELLSSAWTGFFAGCLHTLSGPDHLAALAPLS
Sbjct: 121 APAAFASFQNAAKTGGPAAAAVGRRLIQSELLSSAWTGFFAGCLHTLSGPDHLAALAPLS 180

Query: 181 IGCNRMESAAVGALWGCGHDAGQVLFGLLFLLLKDKLHIEILRTWGTIVVGVTLFVIGVM 240
           IGC+RMESAAVGALWGCGHDAGQV+FGLLFLLLKDKLHIEILRTWGTIVVGVTLFVIGV+
Sbjct: 181 IGCSRMESAAVGALWGCGHDAGQVIFGLLFLLLKDKLHIEILRTWGTIVVGVTLFVIGVL 240

Query: 241 GIREASEVRTPC-VALENGECDVGIYETLENPMVVG--NKKKKKIGFATFATGVVHGLQP 300
           GIREASEVRTPC V LENGECDVGIY+TLE PMV+G  NKKKKKIGFATFATGVVHGLQP
Sbjct: 241 GIREASEVRTPCVVGLENGECDVGIYKTLEKPMVIGEKNKKKKKIGFATFATGVVHGLQP 300

Query: 301 DALMIVLPALALPSRVAGAAFLVMFLVGTVISMGSYTAFIGSFSEALKDRVPRITERLTW 360
           DALMIVLPALALPSRVAGAAFLVMFLVGTVISMGSYTAFIGSFSEALKDRVPRITE+LTW
Sbjct: 301 DALMIVLPALALPSRVAGAAFLVMFLVGTVISMGSYTAFIGSFSEALKDRVPRITEKLTW 360

Query: 361 AASFVAIALGLAIIISQFLGYSLY 382
           AASFVAIALGLAIIISQFLGYSLY
Sbjct: 361 AASFVAIALGLAIIISQFLGYSLY 382

BLAST of Lag0001432 vs. NCBI nr
Match: KAG6589014.1 (hypothetical protein SDJN03_17579, partial [Cucurbita argyrosperma subsp. sororia] >KAG7015277.1 ureH [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 646.4 bits (1666), Expect = 1.6e-181
Identity = 346/384 (90.10%), Postives = 359/384 (93.49%), Query Frame = 0

Query: 1   MDRLLYSPSPSPPSLKFNPNPSLLLPRFARIDSLKLNFAFPMRHGLRPLGFFSFKDEKPS 60
           MDRLLYSPSP+  S K NPNP LLLPRF RIDS KLNF  P RHGLRPLG FS K +KPS
Sbjct: 1   MDRLLYSPSPT--SFKINPNPPLLLPRFRRIDSAKLNFPLPTRHGLRPLGLFSSKVDKPS 60

Query: 61  MSSIRGLSSSNSSSGSICPADSRSGSPKPDLLEHFAGKEKVKKSLFVLSALALILIQPVF 120
           M  I  LSSSNSSS SI  +DSR+ SPKPDLLEHFAGK+KVK+SLFVLSALALILIQPVF
Sbjct: 61  MPLIHCLSSSNSSSDSISTSDSRAVSPKPDLLEHFAGKKKVKQSLFVLSALALILIQPVF 120

Query: 121 APAAFASFQNAAKTGGPAAAAVGRRLIQSELLSSAWTGFFAGCLHTLSGPDHLAALAPLS 180
           APAAFASFQNAAKTGGPAAAAVGRRLIQSELLSSAWTGFFAGCLHTLSGPDHLAALAPLS
Sbjct: 121 APAAFASFQNAAKTGGPAAAAVGRRLIQSELLSSAWTGFFAGCLHTLSGPDHLAALAPLS 180

Query: 181 IGCNRMESAAVGALWGCGHDAGQVLFGLLFLLLKDKLHIEILRTWGTIVVGVTLFVIGVM 240
           IGC+RMESAAVGALWGCGHDAGQ++FGLLFLLLKDKLHIEILRTWGTIVVGVTLFVIGV+
Sbjct: 181 IGCSRMESAAVGALWGCGHDAGQLIFGLLFLLLKDKLHIEILRTWGTIVVGVTLFVIGVL 240

Query: 241 GIREASEVRTPC-VALENGECDVGIYETLENPMVVG--NKKKKKIGFATFATGVVHGLQP 300
           GIREASEVRTPC V LENGECDVGIY+TLENPMV+G  NKKKKKIGFATFATGVVHGLQP
Sbjct: 241 GIREASEVRTPCVVGLENGECDVGIYKTLENPMVIGEKNKKKKKIGFATFATGVVHGLQP 300

Query: 301 DALMIVLPALALPSRVAGAAFLVMFLVGTVISMGSYTAFIGSFSEALKDRVPRITERLTW 360
           DALMIVLPALALPSRVAGAAFLVMFLVGTVISMGSYTAFIGSFSEALKDRVPRITE+LTW
Sbjct: 301 DALMIVLPALALPSRVAGAAFLVMFLVGTVISMGSYTAFIGSFSEALKDRVPRITEKLTW 360

Query: 361 AASFVAIALGLAIIISQFLGYSLY 382
           AASFVAIALGLAIIISQFLGYSLY
Sbjct: 361 AASFVAIALGLAIIISQFLGYSLY 382

BLAST of Lag0001432 vs. NCBI nr
Match: XP_022989236.1 (uncharacterized protein LOC111486376 [Cucurbita maxima])

HSP 1 Score: 646.0 bits (1665), Expect = 2.1e-181
Identity = 346/384 (90.10%), Postives = 358/384 (93.23%), Query Frame = 0

Query: 1   MDRLLYSPSPSPPSLKFNPNPSLLLPRFARIDSLKLNFAFPMRHGLRPLGFFSFKDEKPS 60
           MDRLLYSPSP+  S K NPNP LLLPRF RIDS+KLNF  P RHGLRPLG  S K EKPS
Sbjct: 1   MDRLLYSPSPT--SFKINPNPPLLLPRFRRIDSVKLNFPLPTRHGLRPLGLLSSKVEKPS 60

Query: 61  MSSIRGLSSSNSSSGSICPADSRSGSPKPDLLEHFAGKEKVKKSLFVLSALALILIQPVF 120
           M  I  LSSSNSSS SI  +DSR+ SPKPDLLEHFAGK+KVK+SLFVLSALALILIQPVF
Sbjct: 61  MPLIHCLSSSNSSSDSISTSDSRASSPKPDLLEHFAGKKKVKQSLFVLSALALILIQPVF 120

Query: 121 APAAFASFQNAAKTGGPAAAAVGRRLIQSELLSSAWTGFFAGCLHTLSGPDHLAALAPLS 180
           APAAFASFQNAAKTGGPAAAAVGRRLIQSELLSSAWTGFFAGCLHTLSGPDHLAALAPLS
Sbjct: 121 APAAFASFQNAAKTGGPAAAAVGRRLIQSELLSSAWTGFFAGCLHTLSGPDHLAALAPLS 180

Query: 181 IGCNRMESAAVGALWGCGHDAGQVLFGLLFLLLKDKLHIEILRTWGTIVVGVTLFVIGVM 240
           IGC+RMESAAVGALWGCGHDAGQV+FGLLFLLLKDKLHIEILRTWGTIVVGVTLFVIGV+
Sbjct: 181 IGCSRMESAAVGALWGCGHDAGQVIFGLLFLLLKDKLHIEILRTWGTIVVGVTLFVIGVL 240

Query: 241 GIREASEVRTPC-VALENGECDVGIYETLENPMVVG--NKKKKKIGFATFATGVVHGLQP 300
           GIREASEVRTPC V LENGECDVGIY+TLE PMV+G  NKKKKKIGFATFATGVVHGLQP
Sbjct: 241 GIREASEVRTPCVVGLENGECDVGIYKTLEKPMVIGEKNKKKKKIGFATFATGVVHGLQP 300

Query: 301 DALMIVLPALALPSRVAGAAFLVMFLVGTVISMGSYTAFIGSFSEALKDRVPRITERLTW 360
           DALMIVLPALALPSRVAGAAFLVMFLVGTVISMGSYTAFIGSFSEALKDRVPRITE+LTW
Sbjct: 301 DALMIVLPALALPSRVAGAAFLVMFLVGTVISMGSYTAFIGSFSEALKDRVPRITEKLTW 360

Query: 361 AASFVAIALGLAIIISQFLGYSLY 382
           AASFVAIALGLAIIISQFLGYSLY
Sbjct: 361 AASFVAIALGLAIIISQFLGYSLY 382

BLAST of Lag0001432 vs. NCBI nr
Match: XP_022928346.1 (uncharacterized protein LOC111435202 [Cucurbita moschata])

HSP 1 Score: 642.9 bits (1657), Expect = 1.8e-180
Identity = 344/384 (89.58%), Postives = 358/384 (93.23%), Query Frame = 0

Query: 1   MDRLLYSPSPSPPSLKFNPNPSLLLPRFARIDSLKLNFAFPMRHGLRPLGFFSFKDEKPS 60
           MDRLLYSPSP+  S K NPNP LLLPRF RIDS KLNF  P RHGLRPLG FS K +KPS
Sbjct: 1   MDRLLYSPSPT--SFKINPNPPLLLPRFRRIDSAKLNFPLPTRHGLRPLGLFSSKVDKPS 60

Query: 61  MSSIRGLSSSNSSSGSICPADSRSGSPKPDLLEHFAGKEKVKKSLFVLSALALILIQPVF 120
           M  I  LSSSNS S SI  ++SR+ SPKPDLLEHFAGK+KVK+SLFVLSALALILIQPVF
Sbjct: 61  MPLIHCLSSSNSLSDSISTSNSRAVSPKPDLLEHFAGKKKVKQSLFVLSALALILIQPVF 120

Query: 121 APAAFASFQNAAKTGGPAAAAVGRRLIQSELLSSAWTGFFAGCLHTLSGPDHLAALAPLS 180
           APAAFASFQNAAKTGGPAAAAVGRRLIQSELLSSAWTGFFAGCLHTLSGPDHLAALAPLS
Sbjct: 121 APAAFASFQNAAKTGGPAAAAVGRRLIQSELLSSAWTGFFAGCLHTLSGPDHLAALAPLS 180

Query: 181 IGCNRMESAAVGALWGCGHDAGQVLFGLLFLLLKDKLHIEILRTWGTIVVGVTLFVIGVM 240
           IGC+RMESAAVGALWGCGHDAGQV+FGLLFLLLKDKLHIEILRTWGTIVVGVTLFVIG++
Sbjct: 181 IGCSRMESAAVGALWGCGHDAGQVIFGLLFLLLKDKLHIEILRTWGTIVVGVTLFVIGIL 240

Query: 241 GIREASEVRTPC-VALENGECDVGIYETLENPMVVG--NKKKKKIGFATFATGVVHGLQP 300
           GIREASEVRTPC V LENGECDVGIY+TLENPMV+G  NKKKKKIGFATFATGVVHGLQP
Sbjct: 241 GIREASEVRTPCVVGLENGECDVGIYKTLENPMVIGEKNKKKKKIGFATFATGVVHGLQP 300

Query: 301 DALMIVLPALALPSRVAGAAFLVMFLVGTVISMGSYTAFIGSFSEALKDRVPRITERLTW 360
           DALMIVLPALALPSRVAGAAFLVMFLVGTVISMGSYTAFIGSFSEALKDRVPRITE+LTW
Sbjct: 301 DALMIVLPALALPSRVAGAAFLVMFLVGTVISMGSYTAFIGSFSEALKDRVPRITEKLTW 360

Query: 361 AASFVAIALGLAIIISQFLGYSLY 382
           AASFVAIALGLAIIISQFLGYSLY
Sbjct: 361 AASFVAIALGLAIIISQFLGYSLY 382

BLAST of Lag0001432 vs. NCBI nr
Match: KAG7011768.1 (ureH [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 626.3 bits (1614), Expect = 1.7e-175
Identity = 335/382 (87.70%), Postives = 350/382 (91.62%), Query Frame = 0

Query: 1   MDRLLYSPSPSPPSLKFNPNPSLLLPRFARIDSLKLNFAFPMRHGLRPLGFFSFKDEKPS 60
           M+RLL SPSP+  SLK N N SL LP F RIDSLKLNF  P RHGL+PLG    K+EKPS
Sbjct: 1   MERLLCSPSPT--SLKINLNSSLTLPWFCRIDSLKLNFPLPTRHGLKPLGLTFCKNEKPS 60

Query: 61  MSSIRGLSSSNSSSGSICPADSRSGSPKPDLLEHFAGKEKVKKSLFVLSALALILIQPVF 120
            SS   LSSSNSSS  I P+DS +GSPKPD LEHFAGKEKVK+SL VLSAL LILIQPVF
Sbjct: 61  RSSFCCLSSSNSSSDLIFPSDSLAGSPKPDFLEHFAGKEKVKRSLCVLSALVLILIQPVF 120

Query: 121 APAAFASFQN-AAKTGGPAAAAVGRRLIQSELLSSAWTGFFAGCLHTLSGPDHLAALAPL 180
           APAAFASFQN AAKTGGPAAA VGRRLIQSELLSSAWTGFFAGCLHTLSGPDHLAALAPL
Sbjct: 121 APAAFASFQNAAAKTGGPAAAVVGRRLIQSELLSSAWTGFFAGCLHTLSGPDHLAALAPL 180

Query: 181 SIGCNRMESAAVGALWGCGHDAGQVLFGLLFLLLKDKLHIEILRTWGTIVVGVTLFVIGV 240
           SIGCNRMESAAVGALWGCGHDAGQV+FGLLFLLLKDKLHIEILRTWGTIVVG+TLF+IGV
Sbjct: 181 SIGCNRMESAAVGALWGCGHDAGQVIFGLLFLLLKDKLHIEILRTWGTIVVGMTLFIIGV 240

Query: 241 MGIREASEVRTPCVALENGECDVGIYETLENPMVVGNKKKKKIGFATFATGVVHGLQPDA 300
           MGIRE SEVRTPCVALENGECDVGIYETLE PMVVG K+KKKIGFATFATGVVHGLQPDA
Sbjct: 241 MGIREVSEVRTPCVALENGECDVGIYETLEKPMVVGKKRKKKIGFATFATGVVHGLQPDA 300

Query: 301 LMIVLPALALPSRVAGAAFLVMFLVGTVISMGSYTAFIGSFSEALKDRVPRITERLTWAA 360
           LMIVLPALALPSRVAGAAFL+MFLVGTV+SMGSYTAFIGSFSEALKDRVPRITERLTWAA
Sbjct: 301 LMIVLPALALPSRVAGAAFLLMFLVGTVLSMGSYTAFIGSFSEALKDRVPRITERLTWAA 360

Query: 361 SFVAIALGLAIIISQFLGYSLY 382
           SFVAIALGLAIII+QFLG+SLY
Sbjct: 361 SFVAIALGLAIIITQFLGFSLY 380

BLAST of Lag0001432 vs. ExPASy Swiss-Prot
Match: Q07404 (Urease accessory protein UreH OS=Bacillus sp. (strain TB-90) OX=36824 GN=ureH PE=3 SV=1)

HSP 1 Score: 67.4 bits (163), Expect = 4.0e-10
Identity = 58/225 (25.78%), Postives = 94/225 (41.78%), Query Frame = 0

Query: 148 QSELLSSAWTGFFAGCLHTLSGPDHLAALAPLSIGCNRM-ESAAVGALWGCGHDAGQVLF 207
           Q +LLS    GF  G  H +  PDH+ A++ +     ++  S+  G  WG GH +  ++F
Sbjct: 3   QVDLLSILTLGFVLGIKHAME-PDHVIAVSTIVCQSKKLWRSSLAGVFWGIGHTSTLLIF 62

Query: 208 GLLFLLLKDKLHIEILRTWG---TIVVGVTLFVIGVMGIREASEVRTPCVALENGECDVG 267
           G+  +L+K K+  E    W      +VG+ L   G+  I    +      +  +   D  
Sbjct: 63  GMTIILMKKKISQE----WSMSLEFLVGIILVYFGISAILSLKKTHEHSHSRLHLHTDHP 122

Query: 268 IYETLENPMVVGNKKKKKIGFATFATGVVHGLQPDALMIVLPALALPSRVAGAAFLVMFL 327
           IY     P V            +   G++HGL   A M++L    +     G  +++ F 
Sbjct: 123 IYTYKGIPYV-----------KSLFIGIIHGLAGSAAMVLLTMSTVEKAWEGLLYILFFG 182

Query: 328 VGTVISMGSYTAFIGSFSEALKDRVPRITERLTWAASFVAIALGL 369
            GTV+ M S+T  IG     L  R  RI         F++   G+
Sbjct: 183 AGTVLGMLSFTTLIG-IPFTLSARKIRIHNAFIQITGFISTVFGI 210

BLAST of Lag0001432 vs. ExPASy TrEMBL
Match: A0A6J1JJH7 (uncharacterized protein LOC111486376 OS=Cucurbita maxima OX=3661 GN=LOC111486376 PE=4 SV=1)

HSP 1 Score: 646.0 bits (1665), Expect = 1.0e-181
Identity = 346/384 (90.10%), Postives = 358/384 (93.23%), Query Frame = 0

Query: 1   MDRLLYSPSPSPPSLKFNPNPSLLLPRFARIDSLKLNFAFPMRHGLRPLGFFSFKDEKPS 60
           MDRLLYSPSP+  S K NPNP LLLPRF RIDS+KLNF  P RHGLRPLG  S K EKPS
Sbjct: 1   MDRLLYSPSPT--SFKINPNPPLLLPRFRRIDSVKLNFPLPTRHGLRPLGLLSSKVEKPS 60

Query: 61  MSSIRGLSSSNSSSGSICPADSRSGSPKPDLLEHFAGKEKVKKSLFVLSALALILIQPVF 120
           M  I  LSSSNSSS SI  +DSR+ SPKPDLLEHFAGK+KVK+SLFVLSALALILIQPVF
Sbjct: 61  MPLIHCLSSSNSSSDSISTSDSRASSPKPDLLEHFAGKKKVKQSLFVLSALALILIQPVF 120

Query: 121 APAAFASFQNAAKTGGPAAAAVGRRLIQSELLSSAWTGFFAGCLHTLSGPDHLAALAPLS 180
           APAAFASFQNAAKTGGPAAAAVGRRLIQSELLSSAWTGFFAGCLHTLSGPDHLAALAPLS
Sbjct: 121 APAAFASFQNAAKTGGPAAAAVGRRLIQSELLSSAWTGFFAGCLHTLSGPDHLAALAPLS 180

Query: 181 IGCNRMESAAVGALWGCGHDAGQVLFGLLFLLLKDKLHIEILRTWGTIVVGVTLFVIGVM 240
           IGC+RMESAAVGALWGCGHDAGQV+FGLLFLLLKDKLHIEILRTWGTIVVGVTLFVIGV+
Sbjct: 181 IGCSRMESAAVGALWGCGHDAGQVIFGLLFLLLKDKLHIEILRTWGTIVVGVTLFVIGVL 240

Query: 241 GIREASEVRTPC-VALENGECDVGIYETLENPMVVG--NKKKKKIGFATFATGVVHGLQP 300
           GIREASEVRTPC V LENGECDVGIY+TLE PMV+G  NKKKKKIGFATFATGVVHGLQP
Sbjct: 241 GIREASEVRTPCVVGLENGECDVGIYKTLEKPMVIGEKNKKKKKIGFATFATGVVHGLQP 300

Query: 301 DALMIVLPALALPSRVAGAAFLVMFLVGTVISMGSYTAFIGSFSEALKDRVPRITERLTW 360
           DALMIVLPALALPSRVAGAAFLVMFLVGTVISMGSYTAFIGSFSEALKDRVPRITE+LTW
Sbjct: 301 DALMIVLPALALPSRVAGAAFLVMFLVGTVISMGSYTAFIGSFSEALKDRVPRITEKLTW 360

Query: 361 AASFVAIALGLAIIISQFLGYSLY 382
           AASFVAIALGLAIIISQFLGYSLY
Sbjct: 361 AASFVAIALGLAIIISQFLGYSLY 382

BLAST of Lag0001432 vs. ExPASy TrEMBL
Match: A0A6J1EK24 (uncharacterized protein LOC111435202 OS=Cucurbita moschata OX=3662 GN=LOC111435202 PE=4 SV=1)

HSP 1 Score: 642.9 bits (1657), Expect = 8.5e-181
Identity = 344/384 (89.58%), Postives = 358/384 (93.23%), Query Frame = 0

Query: 1   MDRLLYSPSPSPPSLKFNPNPSLLLPRFARIDSLKLNFAFPMRHGLRPLGFFSFKDEKPS 60
           MDRLLYSPSP+  S K NPNP LLLPRF RIDS KLNF  P RHGLRPLG FS K +KPS
Sbjct: 1   MDRLLYSPSPT--SFKINPNPPLLLPRFRRIDSAKLNFPLPTRHGLRPLGLFSSKVDKPS 60

Query: 61  MSSIRGLSSSNSSSGSICPADSRSGSPKPDLLEHFAGKEKVKKSLFVLSALALILIQPVF 120
           M  I  LSSSNS S SI  ++SR+ SPKPDLLEHFAGK+KVK+SLFVLSALALILIQPVF
Sbjct: 61  MPLIHCLSSSNSLSDSISTSNSRAVSPKPDLLEHFAGKKKVKQSLFVLSALALILIQPVF 120

Query: 121 APAAFASFQNAAKTGGPAAAAVGRRLIQSELLSSAWTGFFAGCLHTLSGPDHLAALAPLS 180
           APAAFASFQNAAKTGGPAAAAVGRRLIQSELLSSAWTGFFAGCLHTLSGPDHLAALAPLS
Sbjct: 121 APAAFASFQNAAKTGGPAAAAVGRRLIQSELLSSAWTGFFAGCLHTLSGPDHLAALAPLS 180

Query: 181 IGCNRMESAAVGALWGCGHDAGQVLFGLLFLLLKDKLHIEILRTWGTIVVGVTLFVIGVM 240
           IGC+RMESAAVGALWGCGHDAGQV+FGLLFLLLKDKLHIEILRTWGTIVVGVTLFVIG++
Sbjct: 181 IGCSRMESAAVGALWGCGHDAGQVIFGLLFLLLKDKLHIEILRTWGTIVVGVTLFVIGIL 240

Query: 241 GIREASEVRTPC-VALENGECDVGIYETLENPMVVG--NKKKKKIGFATFATGVVHGLQP 300
           GIREASEVRTPC V LENGECDVGIY+TLENPMV+G  NKKKKKIGFATFATGVVHGLQP
Sbjct: 241 GIREASEVRTPCVVGLENGECDVGIYKTLENPMVIGEKNKKKKKIGFATFATGVVHGLQP 300

Query: 301 DALMIVLPALALPSRVAGAAFLVMFLVGTVISMGSYTAFIGSFSEALKDRVPRITERLTW 360
           DALMIVLPALALPSRVAGAAFLVMFLVGTVISMGSYTAFIGSFSEALKDRVPRITE+LTW
Sbjct: 301 DALMIVLPALALPSRVAGAAFLVMFLVGTVISMGSYTAFIGSFSEALKDRVPRITEKLTW 360

Query: 361 AASFVAIALGLAIIISQFLGYSLY 382
           AASFVAIALGLAIIISQFLGYSLY
Sbjct: 361 AASFVAIALGLAIIISQFLGYSLY 382

BLAST of Lag0001432 vs. ExPASy TrEMBL
Match: A0A6J1C3Z9 (uncharacterized protein LOC111007714 OS=Momordica charantia OX=3673 GN=LOC111007714 PE=4 SV=1)

HSP 1 Score: 625.2 bits (1611), Expect = 1.8e-175
Identity = 332/381 (87.14%), Postives = 349/381 (91.60%), Query Frame = 0

Query: 1   MDRLLYSPSPSPPSLKFNPNPSLLLPRFARIDSLKLNFAFPMRHGLRPLGFFSFKDEKPS 60
           M+RLLYSPSP+  SLK NP+ SL LPRF  I S KLNF  P RHGLR +G  S K+EKP 
Sbjct: 1   MERLLYSPSPT--SLKINPSRSLFLPRFRLIGSAKLNFPIPTRHGLRSIGLISCKNEKPP 60

Query: 61  MSSIRGLSSSNSSSGSICPADSRSGSPKPDLLEHFAGKEKVKKSLFVLSALALILIQPVF 120
           +SSIR LS  NSSS SICP+DSR+ SPK DLLEHF GKEKVK+SLFVLSALALILIQPVF
Sbjct: 61  LSSIRSLSLFNSSSDSICPSDSRAASPKLDLLEHFGGKEKVKRSLFVLSALALILIQPVF 120

Query: 121 APAAFASFQNAAKTGGPAAAAVGRRLIQSELLSSAWTGFFAGCLHTLSGPDHLAALAPLS 180
           APAA ASF NAAKTG PAAAAVGRRLIQSELL+SAWTGFFAGCLHTLSGPDHLAALAPLS
Sbjct: 121 APAASASFPNAAKTGAPAAAAVGRRLIQSELLNSAWTGFFAGCLHTLSGPDHLAALAPLS 180

Query: 181 IGCNRMESAAVGALWGCGHDAGQVLFGLLFLLLKDKLHIEILRTWGTIVVGVTLFVIGVM 240
           IGCNRMESAAVGALWGCGHDAGQV+FGLLFLLLKDKLHIEILRTWGT+VVGVTLF+IGVM
Sbjct: 181 IGCNRMESAAVGALWGCGHDAGQVIFGLLFLLLKDKLHIEILRTWGTVVVGVTLFIIGVM 240

Query: 241 GIREASEVRTPCVALENGECDVGIYETLENPMVVGNKKKKKIGFATFATGVVHGLQPDAL 300
           GIREASEVRTPCVALENGECDVGI+ETL+ PMV    KKKK+GFATFATGVVHGLQPDAL
Sbjct: 241 GIREASEVRTPCVALENGECDVGIFETLDKPMV---GKKKKLGFATFATGVVHGLQPDAL 300

Query: 301 MIVLPALALPSRVAGAAFLVMFLVGTVISMGSYTAFIGSFSEALKDRVPRITERLTWAAS 360
           MIVLPALALPSR+AGAAFLVMFLVGTVISMGSYTAFIGSFSEALKDRVPRITERLTWAAS
Sbjct: 301 MIVLPALALPSRIAGAAFLVMFLVGTVISMGSYTAFIGSFSEALKDRVPRITERLTWAAS 360

Query: 361 FVAIALGLAIIISQFLGYSLY 382
           FVAIALGLAIIISQFLGYSLY
Sbjct: 361 FVAIALGLAIIISQFLGYSLY 376

BLAST of Lag0001432 vs. ExPASy TrEMBL
Match: A0A6J1I158 (uncharacterized protein LOC111468477 OS=Cucurbita maxima OX=3661 GN=LOC111468477 PE=4 SV=1)

HSP 1 Score: 614.0 bits (1582), Expect = 4.2e-172
Identity = 331/382 (86.65%), Postives = 345/382 (90.31%), Query Frame = 0

Query: 1   MDRLLYSPSPSPPSLKFNPNPSLLLPRFARIDSLKLNFAFPMRHGLRPLGFFSFKDEKPS 60
           M+RLLYSPSP+  SLK N N SL L    RIDSLKLNF  P  HGL+PLG    K+EKPS
Sbjct: 1   MERLLYSPSPT--SLKINLNSSLTLLWSGRIDSLKLNFPLPTLHGLKPLGLTFCKNEKPS 60

Query: 61  MSSIRGLSSSNSSSGSICPADSRSGSPKPDLLEHFAGKEKVKKSLFVLSALALILIQPVF 120
           MSS   LSSSN  S SI P+DS +GSPKPD LEHFAGKEKVK+SL VLSAL LILIQP  
Sbjct: 61  MSSFCCLSSSNWLSDSIFPSDSLAGSPKPDFLEHFAGKEKVKRSLCVLSALVLILIQPGL 120

Query: 121 APAAFASFQN-AAKTGGPAAAAVGRRLIQSELLSSAWTGFFAGCLHTLSGPDHLAALAPL 180
           APAAFASFQN AAKTGGPAAA VGRRLIQSELLSSAWTGFFAGCLHTLSGPDHLAALAPL
Sbjct: 121 APAAFASFQNAAAKTGGPAAAVVGRRLIQSELLSSAWTGFFAGCLHTLSGPDHLAALAPL 180

Query: 181 SIGCNRMESAAVGALWGCGHDAGQVLFGLLFLLLKDKLHIEILRTWGTIVVGVTLFVIGV 240
           SIGCNRMESAAVGALWGCGHDAGQV+FGLLFLLLKDKLHIEILRTWGTIVVG+TLF+IGV
Sbjct: 181 SIGCNRMESAAVGALWGCGHDAGQVIFGLLFLLLKDKLHIEILRTWGTIVVGMTLFIIGV 240

Query: 241 MGIREASEVRTPCVALENGECDVGIYETLENPMVVGNKKKKKIGFATFATGVVHGLQPDA 300
           MGIRE SEVRTPCVALENGECDVGIYETLE PMVVG KKKKKIGFATFATGVVHGLQPDA
Sbjct: 241 MGIREVSEVRTPCVALENGECDVGIYETLEKPMVVGKKKKKKIGFATFATGVVHGLQPDA 300

Query: 301 LMIVLPALALPSRVAGAAFLVMFLVGTVISMGSYTAFIGSFSEALKDRVPRITERLTWAA 360
           LMIVLPALALPSRVAGAAFL+MFLVGTV+SMGSYTAFIGSFSEALKDRVPRITERLTWAA
Sbjct: 301 LMIVLPALALPSRVAGAAFLLMFLVGTVLSMGSYTAFIGSFSEALKDRVPRITERLTWAA 360

Query: 361 SFVAIALGLAIIISQFLGYSLY 382
           SFVAIALGLAIII+ FLG+SLY
Sbjct: 361 SFVAIALGLAIIITHFLGFSLY 380

BLAST of Lag0001432 vs. ExPASy TrEMBL
Match: A0A1S3BJI9 (uncharacterized protein LOC103490284 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103490284 PE=4 SV=1)

HSP 1 Score: 607.4 bits (1565), Expect = 4.0e-170
Identity = 327/383 (85.38%), Postives = 345/383 (90.08%), Query Frame = 0

Query: 1   MDRLLYSPSPSPPSLKFNPNPSLLLPRFAR-IDSLKLNFAFPMRHGLRPLGFFSFKDEKP 60
           M+RLL   S SP SL  NPN SL+LPRFAR I SL+ NF  P RHG  PLGF SFK++KP
Sbjct: 1   MERLLLY-SSSPTSLNINPNSSLVLPRFARIIHSLRPNFPLPTRHGFHPLGFLSFKNDKP 60

Query: 61  SMSSIRGLSSSNSSSGSICPADSRSGSPKPDLLEHFAGKEKVKKSLFVLSALALILIQPV 120
           S SSI   SS NSS+ SI  +DS   SPKPD L+ FA KEKVK SLFVLSALALILIQPV
Sbjct: 61  SFSSIPCFSSPNSSADSIFQSDSPPDSPKPDFLKPFARKEKVKSSLFVLSALALILIQPV 120

Query: 121 FAPAAFASFQNAAKTGGPAAAAVGRRLIQSELLSSAWTGFFAGCLHTLSGPDHLAALAPL 180
           FAPAAFASFQNAAKTGGPAA AVGRRLIQSELL+SAWTGFFAGCLHTLSGPDHLAALAPL
Sbjct: 121 FAPAAFASFQNAAKTGGPAAVAVGRRLIQSELLNSAWTGFFAGCLHTLSGPDHLAALAPL 180

Query: 181 SIGCNRMESAAVGALWGCGHDAGQVLFGLLFLLLKDKLHIEILRTWGTIVVGVTLFVIGV 240
           SIGC+RMESAAVGALWGCGHDAGQV+FGLLFLLLKDKLHIEILRTWGTI+VGVTLFVIG+
Sbjct: 181 SIGCSRMESAAVGALWGCGHDAGQVIFGLLFLLLKDKLHIEILRTWGTIIVGVTLFVIGI 240

Query: 241 MGIREASEVRTP-CVALENGECDVGIYETLENPMVVGNKKKKKIGFATFATGVVHGLQPD 300
           MGIREASE+RTP  VAL+NGECDVGIYETLE PMVVGNKK KK+GFATFATGVVHGLQPD
Sbjct: 241 MGIREASEIRTPFVVALDNGECDVGIYETLEKPMVVGNKKSKKLGFATFATGVVHGLQPD 300

Query: 301 ALMIVLPALALPSRVAGAAFLVMFLVGTVISMGSYTAFIGSFSEALKDRVPRITERLTWA 360
           ALMIVLPALALPSRVAGAAFL+MFLVGTVISMGSYTAFIG+FSEALKDRVPRITERLTW 
Sbjct: 301 ALMIVLPALALPSRVAGAAFLLMFLVGTVISMGSYTAFIGTFSEALKDRVPRITERLTWV 360

Query: 361 ASFVAIALGLAIIISQFLGYSLY 382
           ASFVAIALGLAIIISQFLGYSLY
Sbjct: 361 ASFVAIALGLAIIISQFLGYSLY 382

BLAST of Lag0001432 vs. TAIR 10
Match: AT2G16800.1 (high-affinity nickel-transport family protein )

HSP 1 Score: 395.6 bits (1015), Expect = 4.6e-110
Identity = 231/391 (59.08%), Postives = 281/391 (71.87%), Query Frame = 0

Query: 1   MDRLLYSPSP-SPPSLKFNPNPSLLLPRFARIDSLKLNFAFPMRHGL---RPLGFFSFKD 60
           MDRLL  PS  S    KF   PS LL    R+ S  L+  FP    L   R      F  
Sbjct: 1   MDRLLQPPSSHSIAPSKFQSRPSPLL--LHRLHSTNLS-TFPSSRRLESRRISSISCFFR 60

Query: 61  EKPSMSSIRGLSSSNSSSGSICPADSRSGSPKPDLLEHFAGKEKVKKSL-----FVLSAL 120
           + PS  +  GL  + SS+  I  + + +  P P  ++      + +K++      ++SA+
Sbjct: 61  QNPSPDTSPGL--NQSSNFLIASSQTDASKPNPGFIQRIVSSFEQRKTISAGMVILVSAI 120

Query: 121 ALILIQPVFAPAAFASFQNAAKTGGPAAAAVGRRLIQSELLSSAWTGFFAGCLHTLSGPD 180
           A +L+ P+  P AFASFQ A  +GG  AA VG +L+++E+L+SAWTGFFAGCLHTLSGPD
Sbjct: 121 AALLLNPILVPPAFASFQTATNSGG--AAVVGGKLLRTEVLTSAWTGFFAGCLHTLSGPD 180

Query: 181 HLAALAPLSIGCNRMESAAVGALWGCGHDAGQVLFGLLFLLLKDKLHIEILRTWGTIVVG 240
           HLAALAPLSIG  RMESAAVGALWGCGHDAGQ++FGLLFLLLKD+LHIE++RTWGT VVG
Sbjct: 181 HLAALAPLSIGRTRMESAAVGALWGCGHDAGQLIFGLLFLLLKDRLHIEVIRTWGTRVVG 240

Query: 241 VTLFVIGVMGIREASEVRTPC-VALENGECDVGIYETLENPMVVGNKKKKKIGFATFATG 300
           +TL VIG MGI+EASE+  PC V LENGE D              + KKKKIGFATFATG
Sbjct: 241 LTLLVIGAMGIKEASEMPEPCVVTLENGETD------------EKSLKKKKIGFATFATG 300

Query: 301 VVHGLQPDALMIVLPALALPSRVAGAAFLVMFLVGTVISMGSYTAFIGSFSEALKDRVPR 360
           +VHGLQPDALM+VLPALALPSR+AGA+FL+MFL+GTVI+MGSYT FIGS SEALK++VPR
Sbjct: 301 IVHGLQPDALMMVLPALALPSRIAGASFLIMFLIGTVIAMGSYTVFIGSCSEALKEKVPR 360

Query: 361 ITERLTWAASFVAIALGLAIIISQFLGYSLY 382
           ITE+LTWA+S VAI LGLAII+SQF G+SLY
Sbjct: 361 ITEKLTWASSLVAIGLGLAIIVSQFFGFSLY 372

BLAST of Lag0001432 vs. TAIR 10
Match: AT4G35080.1 (high-affinity nickel-transport family protein )

HSP 1 Score: 375.2 bits (962), Expect = 6.4e-104
Identity = 220/388 (56.70%), Postives = 273/388 (70.36%), Query Frame = 0

Query: 1   MDRLLYSPSPSPPSLKFNPNPSLLLPRFARIDSLKLNFAFPMRHGLRPLGFFSFKDEK-P 60
           M+RLL  PS S  S+  +  PS   P   R+ S  L+F    R   R +   S    + P
Sbjct: 1   MERLL-QPSSSSSSISPSKFPSRTSPFLPRLRSSGLSFVSTHRPESRRVSSISCNSSQIP 60

Query: 61  SMSSIRGLSSSNSSSGSICPADSRSGSPKPDLLEHFAGKEKVKK------SLFVLSALAL 120
           S+ +  G +++N+S      +D     P P  L   A     ++      ++ ++SA+A+
Sbjct: 61  SLYTPIGSNTTNNSFNGSPKSD--ESKPNPGFLTRIATSASEQRKTLSTGTVILISAVAV 120

Query: 121 ILIQPVFAPAAFASFQNAAKTGGPAAAAVGRRLIQSELLSSAWTGFFAGCLHTLSGPDHL 180
           +L+ P+ AP AFASFQ AAK+G                L+SAWTGF AGCLHTLSGPDHL
Sbjct: 121 LLLNPLLAPPAFASFQTAAKSG---------------WLTSAWTGFLAGCLHTLSGPDHL 180

Query: 181 AALAPLSIGCNRMESAAVGALWGCGHDAGQVLFGLLFLLLKDKLHIEILRTWGTIVVGVT 240
           AALAPLSIG ++MESAAVGALWGCGHDAGQV+FGLLFLLLKD+LHIE+L+TWGT +VG+T
Sbjct: 181 AALAPLSIGRSKMESAAVGALWGCGHDAGQVIFGLLFLLLKDRLHIEVLQTWGTRIVGLT 240

Query: 241 LFVIGVMGIREASEVRTPCVALENGECDVGIYETLENPMVVGNKKKKKIGFATFATGVVH 300
           L +IG MGI+EASE+  PCVALE    D+ +  T +  + +   KKKKIGFATFATGVVH
Sbjct: 241 LVIIGAMGIKEASEIPEPCVALET---DISMVSTEKEALPL--PKKKKIGFATFATGVVH 300

Query: 301 GLQPDALMIVLPALALPSRVAGAAFLVMFLVGTVISMGSYTAFIGSFSEALKDRVPRITE 360
           GLQPDALMIVLPALALPSR+AG+AFL+MFLVGTVI+MGSYTAFIGS SEALK++VPRITE
Sbjct: 301 GLQPDALMIVLPALALPSRLAGSAFLIMFLVGTVIAMGSYTAFIGSCSEALKEKVPRITE 360

Query: 361 RLTWAASFVAIALGLAIIISQFLGYSLY 382
           +LTW +S VAI LGL I+IS F G+SLY
Sbjct: 361 KLTWVSSLVAIGLGLGIVISPFFGFSLY 365

BLAST of Lag0001432 vs. TAIR 10
Match: AT4G35080.3 (high-affinity nickel-transport family protein )

HSP 1 Score: 367.1 bits (941), Expect = 1.7e-101
Identity = 223/412 (54.13%), Postives = 275/412 (66.75%), Query Frame = 0

Query: 1   MDRLLYSPSPSPPSLKFNPNPSLLLPRFARIDSLKLNFAFPMRHGLRPLGFFSFKDEK-P 60
           M+RLL  PS S  S+  +  PS   P   R+ S  L+F    R   R +   S    + P
Sbjct: 1   MERLL-QPSSSSSSISPSKFPSRTSPFLPRLRSSGLSFVSTHRPESRRVSSISCNSSQIP 60

Query: 61  SMSSIRGLSSSNSSSGSICPADSRSGSPKPDLLEHFA------GKEKVKK---------- 120
           S+ +  G +++N+S      +D     P P  L   A       KE  +K          
Sbjct: 61  SLYTPIGSNTTNNSFNGSPKSD--ESKPNPGFLTRIATSASEQRKETYEKFVYSLLEKKI 120

Query: 121 --------------SLFVLSALALILIQPVFAPAAFASFQNAAKTGGPAAAAVGRRLIQS 180
                         ++ ++SA+A++L+ P+ AP AFASFQ AAK+G              
Sbjct: 121 PRITKSSNFTLSTGTVILISAVAVLLLNPLLAPPAFASFQTAAKSG-------------- 180

Query: 181 ELLSSAWTGFFAGCLHTLSGPDHLAALAPLSIGCNRMESAAVGALWGCGHDAGQVLFGLL 240
             L+SAWTGF AGCLHTLSGPDHLAALAPLSIG ++MESAAVGALWGCGHDAGQV+FGLL
Sbjct: 181 -WLTSAWTGFLAGCLHTLSGPDHLAALAPLSIGRSKMESAAVGALWGCGHDAGQVIFGLL 240

Query: 241 FLLLKDKLHIEILRTWGTIVVGVTLFVIGVMGIREASEVRTPCVALENGECDVGIYETLE 300
           FLLLKD+LHIE+L+TWGT +VG+TL +IG MGI+EASE+  PCVALE    D+ +  T +
Sbjct: 241 FLLLKDRLHIEVLQTWGTRIVGLTLVIIGAMGIKEASEIPEPCVALET---DISMVSTEK 300

Query: 301 NPMVVGNKKKKKIGFATFATGVVHGLQPDALMIVLPALALPSRVAGAAFLVMFLVGTVIS 360
             + +   KKKKIGFATFATGVVHGLQPDALMIVLPALALPSR+AG+AFL+MFLVGTVI+
Sbjct: 301 EALPL--PKKKKIGFATFATGVVHGLQPDALMIVLPALALPSRLAGSAFLIMFLVGTVIA 360

Query: 361 MGSYTAFIGSFSEALKDRVPRITERLTWAASFVAIALGLAIIISQFLGYSLY 382
           MGSYTAFIGS SEALK++VPRITE+LTW +S VAI LGL I+IS F G+SLY
Sbjct: 361 MGSYTAFIGSCSEALKEKVPRITEKLTWVSSLVAIGLGLGIVISPFFGFSLY 389

BLAST of Lag0001432 vs. TAIR 10
Match: AT4G35080.2 (high-affinity nickel-transport family protein )

HSP 1 Score: 316.6 bits (810), Expect = 2.7e-86
Identity = 196/388 (50.52%), Postives = 245/388 (63.14%), Query Frame = 0

Query: 1   MDRLLYSPSPSPPSLKFNPNPSLLLPRFARIDSLKLNFAFPMRHGLRPLGFFSFKDEK-P 60
           M+RLL  PS S  S+  +  PS   P   R+ S  L+F    R   R +   S    + P
Sbjct: 1   MERLL-QPSSSSSSISPSKFPSRTSPFLPRLRSSGLSFVSTHRPESRRVSSISCNSSQIP 60

Query: 61  SMSSIRGLSSSNSSSGSICPADSRSGSPKPDLLEHFAGKEKVKK------SLFVLSALAL 120
           S+ +  G +++N+S      +D     P P  L   A     ++      ++ ++SA+A+
Sbjct: 61  SLYTPIGSNTTNNSFNGSPKSD--ESKPNPGFLTRIATSASEQRKTLSTGTVILISAVAV 120

Query: 121 ILIQPVFAPAAFASFQNAAKTGGPAAAAVGRRLIQSELLSSAWTGFFAGCLHTLSGPDHL 180
           +L+ P+ AP AFASFQ AAK+G                L+SAWTGF AGCLHTLSGPDHL
Sbjct: 121 LLLNPLLAPPAFASFQTAAKSG---------------WLTSAWTGFLAGCLHTLSGPDHL 180

Query: 181 AALAPLSIGCNRMESAAVGALWGCGHDAGQVLFGLLFLLLKDKLHIEILRTWGTIVVGVT 240
           AALAPLSIG ++MESAAVGALWGCGHDAGQV+FGLLFLLLKD+LHIE+L+TWGT +VG+T
Sbjct: 181 AALAPLSIGRSKMESAAVGALWGCGHDAGQVIFGLLFLLLKDRLHIEVLQTWGTRIVGLT 240

Query: 241 LFVIGVMGIREASEVRTPCVALENGECDVGIYETLENPMVVGNKKKKKIGFATFATGVVH 300
           L +IG MGI+EASE+  PCVALE    D+ +  T +  + +   KKKKIGFATFATGVVH
Sbjct: 241 LVIIGAMGIKEASEIPEPCVALET---DISMVSTEKEALPL--PKKKKIGFATFATGVVH 300

Query: 301 GLQPDALMIVLPALALPSRVAGAAFLVMFLVGTVISMGSYTAFIGSFSEALKDRVPRITE 360
           GLQPDALMIVLPALALPSR                             EALK++VPRITE
Sbjct: 301 GLQPDALMIVLPALALPSR-----------------------------EALKEKVPRITE 336

Query: 361 RLTWAASFVAIALGLAIIISQFLGYSLY 382
           +LTW +S VAI LGL I+IS F G+SLY
Sbjct: 361 KLTWVSSLVAIGLGLGIVISPFFGFSLY 336