Lag0001384 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0001384 vs. NCBI nr
Match: XP_022952337.1 (trans-resveratrol di-O-methyltransferase-like [Cucurbita moschata])

HSP 1 Score: 562.8 bits (1449), Expect = 2.2e-156
Identity = 278/370 (75.14%), Postives = 318/370 (85.95%), Query Frame = 0

Query: 1   MDLENGNITIGASDELLDAQTHIWNHFLSFINSMSLKCAVQLGIADAIHRHGPHPMPLSQ 60
           M+LE G +   A +ELL+AQ HIWNH  +FINSM+LKCA+QLGI DAIHRHGPHPMPLS+
Sbjct: 1   MNLEGGKMAAIAGNELLEAQAHIWNHLFNFINSMTLKCAIQLGIPDAIHRHGPHPMPLSR 60

Query: 61  LVSALKIHPQKSQFIYRLMRILTHSGFFLTQKV-----TTKEKQEEGYMLTNSSRLLLKH 120
           LVSAL++HP K+QFIYRLMR+LTHSGFF+ QK+       +E++EEGY+LTNSSRLLLKH
Sbjct: 61  LVSALQLHPNKTQFIYRLMRLLTHSGFFVAQKLPKNSGLEEEEEEEGYVLTNSSRLLLKH 120

Query: 121 NPLAVTPFLLSMLDAPLVEPWQFLSVWFRSEDRTPYDTAHGTSFWEYMMDHNKSKDGEVF 180
           NP AVTPFLLSML   LVEPWQ LS WF+SEDRTP+ TAHG  FWE++   NK KDGE F
Sbjct: 121 NPCAVTPFLLSMLQPALVEPWQLLSTWFQSEDRTPFHTAHGMPFWEFL--GNKVKDGEGF 180

Query: 181 NAGMASDARLVMSVLLGKYRGVFEGLGSLVDVGGGTGTMAKAIAEAFPEIECIVFDLPQV 240
           NAGMASDARLVMSVLL K++GVF+G+ SLVDVGGGTGT+ KAIAEAFP+IEC VFDLPQV
Sbjct: 181 NAGMASDARLVMSVLLDKHKGVFDGVASLVDVGGGTGTVGKAIAEAFPQIECTVFDLPQV 240

Query: 241 VAGLKGKGNLKYVEGDMFKAIPPADALLLKWILHDWSDEECVKILKKCKTAITSKCNKGK 300
           V  LKG+GNLKYVEGDMFKAIPPADALLLKWILHDWSD+ECVKILK CK AITS   KGK
Sbjct: 241 VGELKGEGNLKYVEGDMFKAIPPADALLLKWILHDWSDQECVKILKNCKAAITSNGYKGK 300

Query: 301 VIVIDMVVGNQ-KKDDSIETQLLFDMLMMILVSGREREEKEWAQLIKKAGFSGYKIFPIM 360
           V+VIDMVVG + K+ DS ETQL ++MLMMILV G+EREEKEWA+LI++AGFSGY I PI+
Sbjct: 301 VMVIDMVVGYKGKESDSYETQLFYEMLMMILVGGKEREEKEWAKLIEEAGFSGYNILPIV 360

Query: 361 GLRSLIEIYP 365
           GLRSLIEIYP
Sbjct: 361 GLRSLIEIYP 368

BLAST of Lag0001384 vs. NCBI nr
Match: KAG6572273.1 (Trans-resveratrol di-O-methyltransferase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 554.7 bits (1428), Expect = 6.0e-154
Identity = 276/371 (74.39%), Postives = 315/371 (84.91%), Query Frame = 0

Query: 1   MDLENGNITIGASDELLDAQTHIWNHFLSFINSMSLKCAVQLGIADAIHRHGPHPMPLSQ 60
           M+LE G +   A +ELL+AQ HIWNH  +FINSM+LKCA+QLGI DAIHRHGP+PMPLS+
Sbjct: 1   MNLEGGKMAAIAGNELLEAQAHIWNHLFNFINSMTLKCAIQLGIPDAIHRHGPNPMPLSR 60

Query: 61  LVSALKIHPQKSQFIYRLMRILTHSGFFLTQK------VTTKEKQEEGYMLTNSSRLLLK 120
           LVSAL++HP K+QFIYRLMR+LTHSGFF+ QK      +  +E++EEGY+LTNSSRLLLK
Sbjct: 61  LVSALQLHPNKTQFIYRLMRLLTHSGFFVAQKLPKNSGLEEEEEEEEGYVLTNSSRLLLK 120

Query: 121 HNPLAVTPFLLSMLDAPLVEPWQFLSVWFRSEDRTPYDTAHGTSFWEYMMDHNKSKDGEV 180
           HNP  VTPFLLSML   LVEPWQ LS WF+SEDRTP+ TAHG   WE++   NK KDGE 
Sbjct: 121 HNPCGVTPFLLSMLQPALVEPWQILSTWFQSEDRTPFHTAHGMPLWEFL--GNKVKDGEG 180

Query: 181 FNAGMASDARLVMSVLLGKYRGVFEGLGSLVDVGGGTGTMAKAIAEAFPEIECIVFDLPQ 240
           FN GMASDARLVMSVLL K++GVF+G+ SLVDVGGGTGT+AKAIA+AFP+IEC V DLPQ
Sbjct: 181 FNEGMASDARLVMSVLLDKHKGVFDGVASLVDVGGGTGTVAKAIAQAFPQIECTVLDLPQ 240

Query: 241 VVAGLKGKGNLKYVEGDMFKAIPPADALLLKWILHDWSDEECVKILKKCKTAITSKCNKG 300
           VVA LKG+GNLKYVEGDMFKAIP ADALLLKWILHDWSDEECVKILK CK AITS   KG
Sbjct: 241 VVAELKGEGNLKYVEGDMFKAIPSADALLLKWILHDWSDEECVKILKNCKAAITSNGYKG 300

Query: 301 KVIVIDMVVGNQ-KKDDSIETQLLFDMLMMILVSGREREEKEWAQLIKKAGFSGYKIFPI 360
           KV+VIDMVVG + K+ DS ETQL +DMLMMILV G+EREEKEWA+LIK+AGFSGY I PI
Sbjct: 301 KVMVIDMVVGYKGKESDSYETQLFYDMLMMILVGGKEREEKEWAKLIKEAGFSGYNILPI 360

Query: 361 MGLRSLIEIYP 365
           +GLRSLIEIYP
Sbjct: 361 VGLRSLIEIYP 369

BLAST of Lag0001384 vs. NCBI nr
Match: XP_022969140.1 (trans-resveratrol di-O-methyltransferase-like [Cucurbita maxima])

HSP 1 Score: 552.4 bits (1422), Expect = 3.0e-153
Identity = 274/370 (74.05%), Postives = 313/370 (84.59%), Query Frame = 0

Query: 1   MDLENGNITIGASDELLDAQTHIWNHFLSFINSMSLKCAVQLGIADAIHRHGPHPMPLSQ 60
           M+LE G +   A +ELL+AQ HIWNH  +FINSM+LKCA+QLGI DAIHRH PHPMPLS+
Sbjct: 1   MNLEGGKMAAIAGNELLEAQAHIWNHLFNFINSMTLKCAIQLGIPDAIHRHEPHPMPLSR 60

Query: 61  LVSALKIHPQKSQFIYRLMRILTHSGFFLTQKV-----TTKEKQEEGYMLTNSSRLLLKH 120
           LVSAL++HP K+QFIYRLMR+LTHSGFF+ QKV       +E++EEGY+LTNSSRLLLKH
Sbjct: 61  LVSALQLHPNKTQFIYRLMRLLTHSGFFVAQKVPKNSGKEEEEEEEGYVLTNSSRLLLKH 120

Query: 121 NPLAVTPFLLSMLDAPLVEPWQFLSVWFRSEDRTPYDTAHGTSFWEYMMDHNKSKDGEVF 180
           NP   TPFLLSML   LVEPWQ LS+WF+SEDRTP+ TAHG   WE++   NK KDGE F
Sbjct: 121 NPCGATPFLLSMLQPALVEPWQLLSIWFQSEDRTPFHTAHGMPLWEFL--GNKVKDGEGF 180

Query: 181 NAGMASDARLVMSVLLGKYRGVFEGLGSLVDVGGGTGTMAKAIAEAFPEIECIVFDLPQV 240
           NAGMASDARLVMSVLL K++GVF+G+ SLVDVGGGTGT+AKAIAEAFP+IEC V DLPQV
Sbjct: 181 NAGMASDARLVMSVLLDKHKGVFDGVASLVDVGGGTGTVAKAIAEAFPQIECTVLDLPQV 240

Query: 241 VAGLKGKGNLKYVEGDMFKAIPPADALLLKWILHDWSDEECVKILKKCKTAITSKCNKGK 300
           V  LKG+GNLKYVEGDMFKAIP ADALLLKWILHDWSD ECV+ILK CK AITS   KGK
Sbjct: 241 VGELKGEGNLKYVEGDMFKAIPSADALLLKWILHDWSDPECVRILKNCKAAITSHGYKGK 300

Query: 301 VIVIDMVVGNQ-KKDDSIETQLLFDMLMMILVSGREREEKEWAQLIKKAGFSGYKIFPIM 360
           V+VIDMVVG + K+ DS ETQL +DMLMMILV G+EREEKEWA+LI++AGFSGY I PI+
Sbjct: 301 VMVIDMVVGYKGKESDSYETQLFYDMLMMILVGGKEREEKEWAKLIREAGFSGYNILPIL 360

Query: 361 GLRSLIEIYP 365
           GLRSLIEIYP
Sbjct: 361 GLRSLIEIYP 368

BLAST of Lag0001384 vs. NCBI nr
Match: XP_022952338.1 (probable O-methyltransferase 3 [Cucurbita moschata])

HSP 1 Score: 551.6 bits (1420), Expect = 5.1e-153
Identity = 270/370 (72.97%), Postives = 313/370 (84.59%), Query Frame = 0

Query: 1   MDLENGNITIGASDELLDAQTHIWNHFLSFINSMSLKCAVQLGIADAIHRHGPHPMPLSQ 60
           M+ E   + I A DE+L+AQ HIWNH  +FINSM+LKCAV+LGI D IHRH PHPMPLS 
Sbjct: 1   MNFEGEKMPILAGDEMLEAQAHIWNHLFNFINSMTLKCAVELGIPDTIHRHAPHPMPLSH 60

Query: 61  LVSALKIHPQKSQFIYRLMRILTHSGFFLTQKV-----TTKEKQEEGYMLTNSSRLLLKH 120
           LVSAL++H  K+QFIYRLMR+LTHSGFF+ QK+       +E++E+GY+LTNSSRLLLKH
Sbjct: 61  LVSALQLHSNKTQFIYRLMRLLTHSGFFVVQKLPKNNGKQEEEEEDGYVLTNSSRLLLKH 120

Query: 121 NPLAVTPFLLSMLDAPLVEPWQFLSVWFRSEDRTPYDTAHGTSFWEYMMDHNKSKDGEVF 180
           NP AVTPFLLSML   LVEPWQ LS+WF+SEDRTP+ TAHG  FWE++   NK KDGE F
Sbjct: 121 NPCAVTPFLLSMLQPALVEPWQLLSIWFQSEDRTPFHTAHGMPFWEFL--GNKVKDGEGF 180

Query: 181 NAGMASDARLVMSVLLGKYRGVFEGLGSLVDVGGGTGTMAKAIAEAFPEIECIVFDLPQV 240
           N GMASDARLVMSVLL K++GVF+G+ SLVDVGGGTGT+AKAIAEAFP+IEC VFDLPQV
Sbjct: 181 NEGMASDARLVMSVLLDKHKGVFDGVASLVDVGGGTGTVAKAIAEAFPQIECTVFDLPQV 240

Query: 241 VAGLKGKGNLKYVEGDMFKAIPPADALLLKWILHDWSDEECVKILKKCKTAITSKCNKGK 300
           V  LKG+GNLKYVEGDMFKAIPPAD L+LKWILHDW+D+EC KILKKCK AI S   KGK
Sbjct: 241 VGELKGEGNLKYVEGDMFKAIPPADVLVLKWILHDWNDDECAKILKKCKEAIRSNGKKGK 300

Query: 301 VIVIDMVVGNQKKDDS-IETQLLFDMLMMILVSGREREEKEWAQLIKKAGFSGYKIFPIM 360
           VIVIDMVVGN K+DD+ I++QL FDMLMM LVSG+ER+EKEW++L K+AGF GYKIFPI+
Sbjct: 301 VIVIDMVVGNNKRDDTFIQSQLFFDMLMMGLVSGKERDEKEWSELFKEAGFGGYKIFPIL 360

Query: 361 GLRSLIEIYP 365
           G RSLIEIYP
Sbjct: 361 GSRSLIEIYP 368

BLAST of Lag0001384 vs. NCBI nr
Match: XP_022135671.1 (probable O-methyltransferase 3 [Momordica charantia])

HSP 1 Score: 550.8 bits (1418), Expect = 8.7e-153
Identity = 269/364 (73.90%), Postives = 310/364 (85.16%), Query Frame = 0

Query: 1   MDLENGNITIGASDELLDAQTHIWNHFLSFINSMSLKCAVQLGIADAIHRHGPHPMPLSQ 60
           M++E G      S ELL+AQ HIWNH  +FINSM+LKCAVQLGI DAIHRHGP+PMPLSQ
Sbjct: 1   MNMELGGDIAVPSHELLEAQAHIWNHIFNFINSMALKCAVQLGIPDAIHRHGPNPMPLSQ 60

Query: 61  LVSALKIHPQKSQFIYRLMRILTHSGFFLTQKVTTKEKQEEGYMLTNSSRLLLKHNPLAV 120
           LVSAL++ PQK+Q+IYRLMR+LTHS FF+T K+ TK  +EE Y+LTNSSRLLL  NP AV
Sbjct: 61  LVSALQLSPQKTQYIYRLMRLLTHSSFFVTHKMMTKNGEEEAYVLTNSSRLLLNDNPSAV 120

Query: 121 TPFLLSMLDAPLVEPWQFLSVWFRSEDRTPYDTAHGTSFWEYMMDHNKSKDGEVFNAGMA 180
           TPFLLSML+  LVEPWQ L+ W +SEDRTP+ T HG  FWE+  +  K KDG+VFN  MA
Sbjct: 121 TPFLLSMLEPALVEPWQLLAAWLQSEDRTPFHTTHGMPFWEFFSE--KPKDGDVFNVAMA 180

Query: 181 SDARLVMSVLLGKYRGVFEGLGSLVDVGGGTGTMAKAIAEAFPEIECIVFDLPQVVAGLK 240
           SDARLVMS+LL KY+GVFEG+GSLVDVGGGTGT+AKAIA+AFP+IECIVFDL QVV  LK
Sbjct: 181 SDARLVMSILLDKYKGVFEGVGSLVDVGGGTGTVAKAIAKAFPQIECIVFDLHQVVGNLK 240

Query: 241 GKGNLKYVEGDMFKAIPPADALLLKWILHDWSDEECVKILKKCKTAITSKCNKGKVIVID 300
           G+ NLKYVEG+MF+A+P ADALLLKWILHDW+DEECV+ILKKCK AIT+   KGK+IVID
Sbjct: 241 GEDNLKYVEGNMFEAVPSADALLLKWILHDWNDEECVEILKKCKEAITNNGKKGKMIVID 300

Query: 301 MVVGNQKKDDSIETQLLFDMLMMILVSGREREEKEWAQLIKKAGFSGYKIFPIMGLRSLI 360
           MVVGN++ DDSIETQL FDMLMM L  G+ER+EKEWAQLIK+AG SGYKIFPIMGLRSLI
Sbjct: 301 MVVGNKQGDDSIETQLFFDMLMMNLAGGKERDEKEWAQLIKEAGLSGYKIFPIMGLRSLI 360

Query: 361 EIYP 365
           EIYP
Sbjct: 361 EIYP 362

BLAST of Lag0001384 vs. ExPASy Swiss-Prot
Match: B0ZB57 (Probable O-methyltransferase 3 OS=Humulus lupulus OX=3486 GN=OMT3 PE=2 SV=1)

HSP 1 Score: 432.6 bits (1111), Expect = 4.5e-120
Identity = 225/359 (62.67%), Postives = 273/359 (76.04%), Query Frame = 0

Query: 13  SDELLDAQTHIWNHFLSFINSMSLKCAVQLGIADAIHRHGPHPMPLSQLVSALKIHPQKS 72
           S E+L AQ HIWN   +FINSMSLKCA+QLGI D I+ HG  PM +SQL  AL I+ +KS
Sbjct: 23  STEILGAQAHIWNQIFNFINSMSLKCAIQLGIPDIINNHG-KPMTISQLTLALPINRKKS 82

Query: 73  QFIYRLMRILTHSGFFLTQKVTTKEK---QEEGYMLTNSSRLLLKHNPLAVTPFLLSMLD 132
             +YRLMRIL HSGFF  QK    E+   +EEGY++T++S+LLLK NP++VTPFLL+MLD
Sbjct: 83  PCVYRLMRILIHSGFFALQKAEVGEEGGGEEEGYVITDASKLLLKDNPMSVTPFLLAMLD 142

Query: 133 APLVEPWQFLSVWFRSEDRTPYDTAHGTSFWEYMMDHNKSKDGEVFNAGMASDARLVMSV 192
             + +PW FLS WF++ D TP+DTA+G +FW+Y    ++ K    FN  MASDARLV SV
Sbjct: 143 PVMTKPWDFLSNWFQNGDPTPFDTANGMAFWDY--GSHEPKLARFFNDAMASDARLVTSV 202

Query: 193 LLGKYRGVFEGLGSLVDVGGGTGTMAKAIAEAFPEIECIVFDLPQVVAGLKGKGNLKYVE 252
           ++ K +GVFEG+ SLVDVGGGTGT+A +IA AFP I+C VFDLP VVA L+G  NL +V 
Sbjct: 203 VIEKCKGVFEGVESLVDVGGGTGTVASSIAAAFPHIQCTVFDLPHVVADLQGGNNLNFVG 262

Query: 253 GDMFKAIPPADALLLKWILHDWSDEECVKILKKCKTAITSKCNK--GKVIVIDMVVGNQK 312
           GDMF  +P  + +LLKWILHDW+DEE VKILKKCK AI SK NK  GKVI+IDM V N+K
Sbjct: 263 GDMFVDVPATEVVLLKWILHDWNDEESVKILKKCKEAI-SKSNKKGGKVIIIDMKVENEK 322

Query: 313 --KDDSIETQLLFDMLMMILVSGREREEKEWAQLIKKAGFSGYKIFPIMGLRSLIEIYP 365
              D+S ETQL FDMLMM LV+GRER EKEWA+L K AGFS YKI PI+GLRSLIE+YP
Sbjct: 323 DEDDESYETQLFFDMLMMALVTGRERNEKEWAKLFKDAGFSNYKITPILGLRSLIEVYP 377

BLAST of Lag0001384 vs. ExPASy Swiss-Prot
Match: B6VJS4 (Trans-resveratrol di-O-methyltransferase OS=Vitis vinifera OX=29760 GN=ROMT PE=1 SV=2)

HSP 1 Score: 428.7 bits (1101), Expect = 6.5e-119
Identity = 221/365 (60.55%), Postives = 270/365 (73.97%), Query Frame = 0

Query: 1   MDLENGNITIGASDELLDAQTHIWNHFLSFINSMSLKCAVQLGIADAIHRHGPHPMPLSQ 60
           MDL NG I    S ELL AQ H+WNH  +FI SMSLKCA+QLGI D IH HG  PM L +
Sbjct: 1   MDLANGVI----SAELLHAQAHVWNHIFNFIKSMSLKCAIQLGIPDIIHNHG-KPMTLPE 60

Query: 61  LVSALKIHPQKSQFIYRLMRILTHSGFFLTQKVTTKEKQEEGYMLTNSSRLLLKHNPLAV 120
           LV+ L +HP++SQ +YRLMRIL HSGF   Q+V  + K+EEGY+LT++SRLLL  + L++
Sbjct: 61  LVAKLPVHPKRSQCVYRLMRILVHSGFLAAQRV-QQGKEEEGYVLTDASRLLLMDDSLSI 120

Query: 121 TPFLLSMLDAPLVEPWQFLSVWFRSEDRTPYDTAHGTSFWEYMMDHNKSKDGEVFNAGMA 180
            P +L+MLD  L +PW +LS WF+++D TP+ TA+  SFW+Y    ++ +    FN  MA
Sbjct: 121 RPLVLAMLDPILTKPWHYLSAWFQNDDPTPFHTAYERSFWDYA--GHEPQLNNSFNEAMA 180

Query: 181 SDARLVMSVLLGKYRGVFEGLGSLVDVGGGTGTMAKAIAEAFPEIECIVFDLPQVVAGLK 240
           SDARL+ SVLL + +GVF GL SLVDVGGGTG +AKAIA AFP + C V DL  VVAGL+
Sbjct: 181 SDARLLTSVLLKEGQGVFAGLNSLVDVGGGTGKVAKAIANAFPHLNCTVLDLSHVVAGLQ 240

Query: 241 GKGNLKYVEGDMFKAIPPADALLLKWILHDWSDEECVKILKKCKTAITSKCNKGKVIVID 300
           G  NL Y  GDMF+AIPPADA+LLKWILHDWS+EECVKILK+C+ AI SK N GKVI+ID
Sbjct: 241 GSKNLNYFAGDMFEAIPPADAILLKWILHDWSNEECVKILKRCREAIPSKENGGKVIIID 300

Query: 301 MV-VGNQKKDDSIETQLLFDMLMMILVSGREREEKEWAQLIKKAGFSGYKIFPIMGLRSL 360
           M+ + NQ    S ETQL FDM MMI   GRER+E EW +L   AGFS YKI PI+GLRSL
Sbjct: 301 MIMMKNQGDYKSTETQLFFDMTMMIFAPGRERDENEWEKLFLDAGFSHYKITPILGLRSL 357

Query: 361 IEIYP 365
           IE+YP
Sbjct: 361 IEVYP 357

BLAST of Lag0001384 vs. ExPASy Swiss-Prot
Match: A0A0N9HMN6 (Pluviatolide O-methyltransferase OS=Sinopodophyllum hexandrum OX=93608 GN=OMT3 PE=1 SV=1)

HSP 1 Score: 386.7 bits (992), Expect = 2.8e-106
Identity = 194/369 (52.57%), Postives = 261/369 (70.73%), Query Frame = 0

Query: 1   MDLE--NGNITIGASDELLDAQTHIWNHFLSFINSMSLKCAVQLGIADAIHRHGPHPMPL 60
           MDLE  NGN    + +ELL AQ HI+NH  +FI+SM+LKCAV+L I + +H H P  + L
Sbjct: 7   MDLEIRNGNGYGDSGEELLAAQAHIYNHIFNFISSMALKCAVELNIPEILHNHQPKAVTL 66

Query: 61  SQLVSALKIHPQKSQFIYRLMRILTHSGFFLTQKVTTKEKQEEGYMLTNSSRLLLKHNPL 120
           S+LV AL+I   KS  +YRL+RIL HSGFF   K+ + E  EEGY+ T SS+LLLK++P+
Sbjct: 67  SELVQALQIPQAKSACLYRLLRILVHSGFFAITKIQS-EGDEEGYLPTLSSKLLLKNHPM 126

Query: 121 AVTPFLLSMLDAPLVEPWQFLSVWF-RSEDRTPYDTAHGTSFWEYMMDHNKSKDGEVFNA 180
           +++P LL +++  +V P  F S WF RS+D TP++  HG S W+Y  +       E+FN 
Sbjct: 127 SMSPCLLGLVNPTMVAPMHFFSDWFKRSDDMTPFEATHGASLWKYFGE--TPHMAEIFNE 186

Query: 181 GMASDARLVMSVLLGKYRGVFEGLGSLVDVGGGTGTMAKAIAEAFPEIECIVFDLPQVVA 240
            M  + RL MSV+L + +G  EG+ SLVDVGGGTG + +AIAEAFP ++C V DLPQVV 
Sbjct: 187 AMGCETRLAMSVVLKECKGKLEGISSLVDVGGGTGNVGRAIAEAFPNVKCTVLDLPQVVG 246

Query: 241 GLKGKGNLKYVEGDMFKAIPPADALLLKWILHDWSDEECVKILKKCKTAITSKCNKGKVI 300
            LKG  NL++V GDMF+ IPPAD + LKWILHDW+DEEC+KILK+CK AI SK   GK+I
Sbjct: 247 NLKGSNNLEFVSGDMFQFIPPADVVFLKWILHDWNDEECIKILKRCKEAIPSKEEGGKLI 306

Query: 301 VIDMVVGNQKKD--DSIETQLLFDMLMMILVSGREREEKEWAQLIKKAGFSGYKIFPIMG 360
           +IDMVV +  K   +S ETQL +D+ +M L++G ER E EW +L   AGF+ Y I P++G
Sbjct: 307 IIDMVVNDHNKGSYESTETQLFYDLTLMALLTGTERTETEWKKLFVAAGFTSYIISPVLG 366

Query: 361 LRSLIEIYP 365
           L+S+IE++P
Sbjct: 367 LKSIIEVFP 372

BLAST of Lag0001384 vs. ExPASy Swiss-Prot
Match: Q6VMW0 (8-hydroxyquercetin 8-O-methyltransferase OS=Mentha piperita OX=34256 GN=OMT2 PE=1 SV=1)

HSP 1 Score: 373.2 bits (957), Expect = 3.3e-102
Identity = 195/372 (52.42%), Postives = 258/372 (69.35%), Query Frame = 0

Query: 1   MDLENGNITIGASDELLDAQTHIWNHFLSFINSMSLKCAVQLGIADAIHRHGPHPMPLSQ 60
           M L NG   I +  ELL+AQ H+WNH  S+INSMSLKCA+QLGI DAIH+HG +P+ LSQ
Sbjct: 1   MALPNG---ISSKQELLEAQAHVWNHIYSYINSMSLKCAIQLGIPDAIHKHG-NPITLSQ 60

Query: 61  LVSALKIHPQKSQFIYRLMRILTHSGFFLTQKVTTK------EKQEEGYMLTNSSRLLLK 120
           L  AL I+  KS  ++RLMRIL HSGFF   KV  K      E++E+ Y LT +SRLLL+
Sbjct: 61  LADALNINKAKSHGLFRLMRILVHSGFFDKVKVKVKVEGEDEEEEEDAYSLTPASRLLLR 120

Query: 121 HNPLAVTPFLLSMLDAPLVEPWQFLSVWFRSEDRTPYDTAHGTSFWEYMMDHNKSKDGEV 180
             PL+V PF L+M D    E W  LS WFR++    +DT +G +F EY +  ++     +
Sbjct: 121 SEPLSVAPFALAMSDPVYTETWHHLSEWFRNDAVAAFDTKYGMTFPEYAVADDRL--NVL 180

Query: 181 FNAGMASDARLVMSVLLGKYRGVFEGLGSLVDVGGGTGTMAKAIAEAFPEIECIVFDLPQ 240
           FN  MA DA  V S+L  + R +F+GL S+VDVGGGTG  AK IA AFP +EC V DLP 
Sbjct: 181 FNEAMACDAGFVNSILTTECREIFDGLESMVDVGGGTGATAKGIAAAFPGMECTVLDLPN 240

Query: 241 VVAGLKGKGNLKYVEGDMFKAIPPADALLLKWILHDWSDEECVKILKKCKTAITSKCNK- 300
           VV GLKG  NL +V GDMF  IP ADA+ +K+ILHDW+DEECVKILKKCK AI+   N  
Sbjct: 241 VVGGLKGSENLSFVSGDMFDFIPHADAIFMKFILHDWNDEECVKILKKCKEAISRSNNSC 300

Query: 301 GKVIVIDMVVGNQKK-DDSIETQLLFDMLMMILVSGREREEKEWAQLIKKAGFSGYKIFP 360
            K+I++++V+ ++K+  ++ ET+L FDM M+ +++G+ER EKEW +L   AGF+ YKI  
Sbjct: 301 RKIILVEIVMEDEKETHEATETKLFFDMQMLAIITGKERSEKEWGKLFFDAGFTNYKITR 360

Query: 361 IMGLRSLIEIYP 365
           ++GLRS+IE++P
Sbjct: 361 VLGLRSVIEVFP 366

BLAST of Lag0001384 vs. ExPASy Swiss-Prot
Match: B0EXJ8 (Tabersonine 16-O-methyltransferase OS=Catharanthus roseus OX=4058 GN=16OMT PE=1 SV=1)

HSP 1 Score: 354.4 bits (908), Expect = 1.6e-96
Identity = 187/358 (52.23%), Postives = 253/358 (70.67%), Query Frame = 0

Query: 13  SDELLDAQTHIWNHFLSFINSMSLKCAVQLGIADAIHRHGPHPMPLSQLVSAL--KIHPQ 72
           S+E   AQ  IW+   SFI S SLKCAV+LGI D I  HG  P+ LS+L +AL   +HP 
Sbjct: 5   SEEFRGAQAQIWSQSCSFITSASLKCAVKLGIPDTIDNHG-KPITLSELTNALVPPVHPS 64

Query: 73  KSQFIYRLMRILTHSGFFLTQKVTTKEKQEEGYMLTNSSRLLLKHNPLAVTPFLLSMLDA 132
           K+ FIYRLMR+L  +GF   +++    + E  Y LT SSR+LLK  PL +   +L+M D 
Sbjct: 65  KAPFIYRLMRVLAKNGFCSEEQL--DGETEPLYSLTPSSRILLKKEPLNLRGIVLTMADP 124

Query: 133 PLVEPWQFLSVWFRSED--RTPYDTAHGTSFWEYMMDHNKSKDGEVFNAGMASDARLVMS 192
             ++ W+ LS W+++ED   T ++TAHG +FW Y  +H   +  E FN  MASD++L+  
Sbjct: 125 VQLKAWESLSDWYQNEDDSSTAFETAHGKNFWGYSSEH--MEHAEFFNEAMASDSQLISK 184

Query: 193 VLLGKYRGVFEGLGSLVDVGGGTGTMAKAIAEAFPEIECIVFDLPQVVAGLKGKGNLKYV 252
           +L+G+Y+ +FEGL SLVD+GGGTGT+AKAIA+ FP+++C VFDLP VVA L+ K N+++V
Sbjct: 185 LLIGEYKFLFEGLASLVDIGGGTGTIAKAIAKNFPQLKCTVFDLPHVVANLESKENVEFV 244

Query: 253 EGDMFKAIPPADALLLKWILHDWSDEECVKILKKCKTAITSKCNKGKVIVIDMVVGNQKK 312
            GDMF+ IP A+A+ LKWILHDW+DE+CVKILK CK AI +K   GKVI+IDMV+ + KK
Sbjct: 245 AGDMFEKIPSANAIFLKWILHDWNDEDCVKILKSCKKAIPAK--GGKVIIIDMVMYSDKK 304

Query: 313 DDS-IETQLLFDMLMMILVSGREREEKEWAQLIKKAGFSGYKIFPIMGL-RSLIEIYP 365
           DD  ++TQ   DM M++  + +ER EKEWA L K+AGFS YKI+P +   RSLIE+YP
Sbjct: 305 DDHLVKTQTSMDMAMLVNFAAKERCEKEWAFLFKEAGFSDYKIYPKLDFTRSLIEVYP 355

BLAST of Lag0001384 vs. ExPASy TrEMBL
Match: A0A6J1GK49 (trans-resveratrol di-O-methyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111455045 PE=3 SV=1)

HSP 1 Score: 562.8 bits (1449), Expect = 1.1e-156
Identity = 278/370 (75.14%), Postives = 318/370 (85.95%), Query Frame = 0

Query: 1   MDLENGNITIGASDELLDAQTHIWNHFLSFINSMSLKCAVQLGIADAIHRHGPHPMPLSQ 60
           M+LE G +   A +ELL+AQ HIWNH  +FINSM+LKCA+QLGI DAIHRHGPHPMPLS+
Sbjct: 1   MNLEGGKMAAIAGNELLEAQAHIWNHLFNFINSMTLKCAIQLGIPDAIHRHGPHPMPLSR 60

Query: 61  LVSALKIHPQKSQFIYRLMRILTHSGFFLTQKV-----TTKEKQEEGYMLTNSSRLLLKH 120
           LVSAL++HP K+QFIYRLMR+LTHSGFF+ QK+       +E++EEGY+LTNSSRLLLKH
Sbjct: 61  LVSALQLHPNKTQFIYRLMRLLTHSGFFVAQKLPKNSGLEEEEEEEGYVLTNSSRLLLKH 120

Query: 121 NPLAVTPFLLSMLDAPLVEPWQFLSVWFRSEDRTPYDTAHGTSFWEYMMDHNKSKDGEVF 180
           NP AVTPFLLSML   LVEPWQ LS WF+SEDRTP+ TAHG  FWE++   NK KDGE F
Sbjct: 121 NPCAVTPFLLSMLQPALVEPWQLLSTWFQSEDRTPFHTAHGMPFWEFL--GNKVKDGEGF 180

Query: 181 NAGMASDARLVMSVLLGKYRGVFEGLGSLVDVGGGTGTMAKAIAEAFPEIECIVFDLPQV 240
           NAGMASDARLVMSVLL K++GVF+G+ SLVDVGGGTGT+ KAIAEAFP+IEC VFDLPQV
Sbjct: 181 NAGMASDARLVMSVLLDKHKGVFDGVASLVDVGGGTGTVGKAIAEAFPQIECTVFDLPQV 240

Query: 241 VAGLKGKGNLKYVEGDMFKAIPPADALLLKWILHDWSDEECVKILKKCKTAITSKCNKGK 300
           V  LKG+GNLKYVEGDMFKAIPPADALLLKWILHDWSD+ECVKILK CK AITS   KGK
Sbjct: 241 VGELKGEGNLKYVEGDMFKAIPPADALLLKWILHDWSDQECVKILKNCKAAITSNGYKGK 300

Query: 301 VIVIDMVVGNQ-KKDDSIETQLLFDMLMMILVSGREREEKEWAQLIKKAGFSGYKIFPIM 360
           V+VIDMVVG + K+ DS ETQL ++MLMMILV G+EREEKEWA+LI++AGFSGY I PI+
Sbjct: 301 VMVIDMVVGYKGKESDSYETQLFYEMLMMILVGGKEREEKEWAKLIEEAGFSGYNILPIV 360

Query: 361 GLRSLIEIYP 365
           GLRSLIEIYP
Sbjct: 361 GLRSLIEIYP 368

BLAST of Lag0001384 vs. ExPASy TrEMBL
Match: A0A6J1HZ45 (trans-resveratrol di-O-methyltransferase-like OS=Cucurbita maxima OX=3661 GN=LOC111468224 PE=3 SV=1)

HSP 1 Score: 552.4 bits (1422), Expect = 1.4e-153
Identity = 274/370 (74.05%), Postives = 313/370 (84.59%), Query Frame = 0

Query: 1   MDLENGNITIGASDELLDAQTHIWNHFLSFINSMSLKCAVQLGIADAIHRHGPHPMPLSQ 60
           M+LE G +   A +ELL+AQ HIWNH  +FINSM+LKCA+QLGI DAIHRH PHPMPLS+
Sbjct: 1   MNLEGGKMAAIAGNELLEAQAHIWNHLFNFINSMTLKCAIQLGIPDAIHRHEPHPMPLSR 60

Query: 61  LVSALKIHPQKSQFIYRLMRILTHSGFFLTQKV-----TTKEKQEEGYMLTNSSRLLLKH 120
           LVSAL++HP K+QFIYRLMR+LTHSGFF+ QKV       +E++EEGY+LTNSSRLLLKH
Sbjct: 61  LVSALQLHPNKTQFIYRLMRLLTHSGFFVAQKVPKNSGKEEEEEEEGYVLTNSSRLLLKH 120

Query: 121 NPLAVTPFLLSMLDAPLVEPWQFLSVWFRSEDRTPYDTAHGTSFWEYMMDHNKSKDGEVF 180
           NP   TPFLLSML   LVEPWQ LS+WF+SEDRTP+ TAHG   WE++   NK KDGE F
Sbjct: 121 NPCGATPFLLSMLQPALVEPWQLLSIWFQSEDRTPFHTAHGMPLWEFL--GNKVKDGEGF 180

Query: 181 NAGMASDARLVMSVLLGKYRGVFEGLGSLVDVGGGTGTMAKAIAEAFPEIECIVFDLPQV 240
           NAGMASDARLVMSVLL K++GVF+G+ SLVDVGGGTGT+AKAIAEAFP+IEC V DLPQV
Sbjct: 181 NAGMASDARLVMSVLLDKHKGVFDGVASLVDVGGGTGTVAKAIAEAFPQIECTVLDLPQV 240

Query: 241 VAGLKGKGNLKYVEGDMFKAIPPADALLLKWILHDWSDEECVKILKKCKTAITSKCNKGK 300
           V  LKG+GNLKYVEGDMFKAIP ADALLLKWILHDWSD ECV+ILK CK AITS   KGK
Sbjct: 241 VGELKGEGNLKYVEGDMFKAIPSADALLLKWILHDWSDPECVRILKNCKAAITSHGYKGK 300

Query: 301 VIVIDMVVGNQ-KKDDSIETQLLFDMLMMILVSGREREEKEWAQLIKKAGFSGYKIFPIM 360
           V+VIDMVVG + K+ DS ETQL +DMLMMILV G+EREEKEWA+LI++AGFSGY I PI+
Sbjct: 301 VMVIDMVVGYKGKESDSYETQLFYDMLMMILVGGKEREEKEWAKLIREAGFSGYNILPIL 360

Query: 361 GLRSLIEIYP 365
           GLRSLIEIYP
Sbjct: 361 GLRSLIEIYP 368

BLAST of Lag0001384 vs. ExPASy TrEMBL
Match: A0A6J1GKA3 (probable O-methyltransferase 3 OS=Cucurbita moschata OX=3662 GN=LOC111455047 PE=3 SV=1)

HSP 1 Score: 551.6 bits (1420), Expect = 2.5e-153
Identity = 270/370 (72.97%), Postives = 313/370 (84.59%), Query Frame = 0

Query: 1   MDLENGNITIGASDELLDAQTHIWNHFLSFINSMSLKCAVQLGIADAIHRHGPHPMPLSQ 60
           M+ E   + I A DE+L+AQ HIWNH  +FINSM+LKCAV+LGI D IHRH PHPMPLS 
Sbjct: 1   MNFEGEKMPILAGDEMLEAQAHIWNHLFNFINSMTLKCAVELGIPDTIHRHAPHPMPLSH 60

Query: 61  LVSALKIHPQKSQFIYRLMRILTHSGFFLTQKV-----TTKEKQEEGYMLTNSSRLLLKH 120
           LVSAL++H  K+QFIYRLMR+LTHSGFF+ QK+       +E++E+GY+LTNSSRLLLKH
Sbjct: 61  LVSALQLHSNKTQFIYRLMRLLTHSGFFVVQKLPKNNGKQEEEEEDGYVLTNSSRLLLKH 120

Query: 121 NPLAVTPFLLSMLDAPLVEPWQFLSVWFRSEDRTPYDTAHGTSFWEYMMDHNKSKDGEVF 180
           NP AVTPFLLSML   LVEPWQ LS+WF+SEDRTP+ TAHG  FWE++   NK KDGE F
Sbjct: 121 NPCAVTPFLLSMLQPALVEPWQLLSIWFQSEDRTPFHTAHGMPFWEFL--GNKVKDGEGF 180

Query: 181 NAGMASDARLVMSVLLGKYRGVFEGLGSLVDVGGGTGTMAKAIAEAFPEIECIVFDLPQV 240
           N GMASDARLVMSVLL K++GVF+G+ SLVDVGGGTGT+AKAIAEAFP+IEC VFDLPQV
Sbjct: 181 NEGMASDARLVMSVLLDKHKGVFDGVASLVDVGGGTGTVAKAIAEAFPQIECTVFDLPQV 240

Query: 241 VAGLKGKGNLKYVEGDMFKAIPPADALLLKWILHDWSDEECVKILKKCKTAITSKCNKGK 300
           V  LKG+GNLKYVEGDMFKAIPPAD L+LKWILHDW+D+EC KILKKCK AI S   KGK
Sbjct: 241 VGELKGEGNLKYVEGDMFKAIPPADVLVLKWILHDWNDDECAKILKKCKEAIRSNGKKGK 300

Query: 301 VIVIDMVVGNQKKDDS-IETQLLFDMLMMILVSGREREEKEWAQLIKKAGFSGYKIFPIM 360
           VIVIDMVVGN K+DD+ I++QL FDMLMM LVSG+ER+EKEW++L K+AGF GYKIFPI+
Sbjct: 301 VIVIDMVVGNNKRDDTFIQSQLFFDMLMMGLVSGKERDEKEWSELFKEAGFGGYKIFPIL 360

Query: 361 GLRSLIEIYP 365
           G RSLIEIYP
Sbjct: 361 GSRSLIEIYP 368

BLAST of Lag0001384 vs. ExPASy TrEMBL
Match: A0A6J1C1F3 (probable O-methyltransferase 3 OS=Momordica charantia OX=3673 GN=LOC111007572 PE=3 SV=1)

HSP 1 Score: 550.8 bits (1418), Expect = 4.2e-153
Identity = 269/364 (73.90%), Postives = 310/364 (85.16%), Query Frame = 0

Query: 1   MDLENGNITIGASDELLDAQTHIWNHFLSFINSMSLKCAVQLGIADAIHRHGPHPMPLSQ 60
           M++E G      S ELL+AQ HIWNH  +FINSM+LKCAVQLGI DAIHRHGP+PMPLSQ
Sbjct: 1   MNMELGGDIAVPSHELLEAQAHIWNHIFNFINSMALKCAVQLGIPDAIHRHGPNPMPLSQ 60

Query: 61  LVSALKIHPQKSQFIYRLMRILTHSGFFLTQKVTTKEKQEEGYMLTNSSRLLLKHNPLAV 120
           LVSAL++ PQK+Q+IYRLMR+LTHS FF+T K+ TK  +EE Y+LTNSSRLLL  NP AV
Sbjct: 61  LVSALQLSPQKTQYIYRLMRLLTHSSFFVTHKMMTKNGEEEAYVLTNSSRLLLNDNPSAV 120

Query: 121 TPFLLSMLDAPLVEPWQFLSVWFRSEDRTPYDTAHGTSFWEYMMDHNKSKDGEVFNAGMA 180
           TPFLLSML+  LVEPWQ L+ W +SEDRTP+ T HG  FWE+  +  K KDG+VFN  MA
Sbjct: 121 TPFLLSMLEPALVEPWQLLAAWLQSEDRTPFHTTHGMPFWEFFSE--KPKDGDVFNVAMA 180

Query: 181 SDARLVMSVLLGKYRGVFEGLGSLVDVGGGTGTMAKAIAEAFPEIECIVFDLPQVVAGLK 240
           SDARLVMS+LL KY+GVFEG+GSLVDVGGGTGT+AKAIA+AFP+IECIVFDL QVV  LK
Sbjct: 181 SDARLVMSILLDKYKGVFEGVGSLVDVGGGTGTVAKAIAKAFPQIECIVFDLHQVVGNLK 240

Query: 241 GKGNLKYVEGDMFKAIPPADALLLKWILHDWSDEECVKILKKCKTAITSKCNKGKVIVID 300
           G+ NLKYVEG+MF+A+P ADALLLKWILHDW+DEECV+ILKKCK AIT+   KGK+IVID
Sbjct: 241 GEDNLKYVEGNMFEAVPSADALLLKWILHDWNDEECVEILKKCKEAITNNGKKGKMIVID 300

Query: 301 MVVGNQKKDDSIETQLLFDMLMMILVSGREREEKEWAQLIKKAGFSGYKIFPIMGLRSLI 360
           MVVGN++ DDSIETQL FDMLMM L  G+ER+EKEWAQLIK+AG SGYKIFPIMGLRSLI
Sbjct: 301 MVVGNKQGDDSIETQLFFDMLMMNLAGGKERDEKEWAQLIKEAGLSGYKIFPIMGLRSLI 360

Query: 361 EIYP 365
           EIYP
Sbjct: 361 EIYP 362

BLAST of Lag0001384 vs. ExPASy TrEMBL
Match: A0A6J1GJY8 (probable O-methyltransferase 3 OS=Cucurbita moschata OX=3662 GN=LOC111455044 PE=3 SV=1)

HSP 1 Score: 550.1 bits (1416), Expect = 7.2e-153
Identity = 268/369 (72.63%), Postives = 319/369 (86.45%), Query Frame = 0

Query: 1   MDLENGN-ITIGASDELLDAQTHIWNHFLSFINSMSLKCAVQLGIADAIHRHGPHPMPLS 60
           M+ E+ N + I A DELL+AQ HIWNH L+FINSM+LKCAV+LGI DAIHRHGPHPMPLS
Sbjct: 5   MNFESENMLPIVAGDELLEAQAHIWNHLLNFINSMTLKCAVELGIPDAIHRHGPHPMPLS 64

Query: 61  QLVSALKIHPQKSQFIYRLMRILTHSGFFLTQKVT----TKEKQEEGYMLTNSSRLLLKH 120
           +LVSAL++HP K++FIYRLMR+LTHSGFF+ QK+      +E++EEGY+LTNSSRLLLK+
Sbjct: 65  RLVSALQLHPHKTRFIYRLMRLLTHSGFFVLQKLPKNSGEEEEEEEGYVLTNSSRLLLKY 124

Query: 121 NPLAVTPFLLSMLDAPLVEPWQFLSVWFRSEDRTPYDTAHGTSFWEYMMDHNKSKDGEVF 180
           NP  VTP LLS+L   LVEPW  LSVWF+SEDRTP+DTAHG S+WE+M   +K K G++F
Sbjct: 125 NPCGVTPLLLSILQPALVEPWHLLSVWFKSEDRTPFDTAHGISYWEFM--GSKPKHGDIF 184

Query: 181 NAGMASDARLVMSVLLGKYRGVFEGLGSLVDVGGGTGTMAKAIAEAFPEIECIVFDLPQV 240
           NAGMASDARLVMSVLL K++GVF+G+ SLVDVGGGTGT+ KAIAEAFP+IEC VFDLPQV
Sbjct: 185 NAGMASDARLVMSVLLDKHKGVFDGVASLVDVGGGTGTVGKAIAEAFPQIECAVFDLPQV 244

Query: 241 VAGLKGKGNLKYVEGDMFKAIPPADALLLKWILHDWSDEECVKILKKCKTAITSKCNKGK 300
           V  LKG+GNLKYVEGDMFKAIPPAD L+LKWILHDW+DE+CVKILK CK AITS   KGK
Sbjct: 245 VGELKGEGNLKYVEGDMFKAIPPADVLVLKWILHDWNDEDCVKILKNCKEAITSNGKKGK 304

Query: 301 VIVIDMVVGNQKKDDSIETQLLFDMLMMILVSGREREEKEWAQLIKKAGFSGYKIFPIMG 360
           VIVIDMVVG++ +D  I++QL FDM+MM L++GRER+EKEW++  K+AGFSGYKIFPI+G
Sbjct: 305 VIVIDMVVGSKLEDTFIQSQLCFDMVMMGLLNGRERDEKEWSEAFKEAGFSGYKIFPILG 364

Query: 361 LRSLIEIYP 365
           LRSLIEIYP
Sbjct: 365 LRSLIEIYP 371

BLAST of Lag0001384 vs. TAIR 10
Match: AT4G35160.1 (O-methyltransferase family protein )

HSP 1 Score: 224.2 bits (570), Expect = 1.7e-58
Identity = 134/366 (36.61%), Postives = 203/366 (55.46%), Query Frame = 0

Query: 14  DELLDAQTHIWNHFLSFINSMSLKCAVQLGIADAIHRH-GPHPMPLSQLVSALKIHPQKS 73
           DE   A   IW +   F +  + KCA+ L I +AI  H    P+ L++L SA+   P   
Sbjct: 24  DEEAKASLDIWKYVFGFADIAAAKCAIDLKIPEAIENHPSSQPVTLAELSSAVSASP--- 83

Query: 74  QFIYRLMRILTHSGFFLTQKVTTKEKQEEGYMLTNSSRLLL--KHNPLAVTPFLLSMLDA 133
             + R+MR L H G F  +++ TK+    GY+ T  SR L+  + +  ++ PF+L     
Sbjct: 84  SHLRRIMRFLVHQGIF--KEIPTKDGLATGYVNTPLSRRLMITRRDGKSLAPFVLFETTP 143

Query: 134 PLVEPWQFLSVWFRS----EDRTPYDTAHGTSFWEYMMDHNKSKDGEVFNAGMASDARLV 193
            ++ PW  LS    S        P+D  HG   W +  D+    D  + N  MA DAR V
Sbjct: 144 EMLAPWLRLSSVVSSPVNGSTPPPFDAVHGKDVWSFAQDNPFLSD--MINEAMACDARRV 203

Query: 194 MSVLLGKYRGVFEGLGSLVDVGGGTGTMAKAIAEAFPEIECIVFDLPQVVAGLKGKGNLK 253
           +  + G   G+F+G+ ++VDVGGGTG     + + FP I+   FDLP V+   +    ++
Sbjct: 204 VPRVAGACHGLFDGVTTMVDVGGGTGETMGMLVKEFPWIKGFNFDLPHVIEVAEVLDGVE 263

Query: 254 YVEGDMFKAIPPADALLLKWILHDWSDEECVKILKKCKTAITSKCNKGKVIVIDMVVGNQ 313
            VEGDMF +IP  DA+ +KW+LHDW D++C+KILK CK A+    N GKV++++ V+G  
Sbjct: 264 NVEGDMFDSIPACDAIFIKWVLHDWGDKDCIKILKNCKEAVPP--NIGKVLIVESVIGEN 323

Query: 314 KK-------DDSIE-TQLLFDMLMMILVS-GREREEKEWAQLIKKAGFSGYKIFPIMGLR 364
           KK       D+ +E  +L+ DM+MM   S G+ER  KEW  ++K+AGF+ Y++  I  ++
Sbjct: 324 KKTMIVDERDEKLEHVRLMLDMVMMAHTSTGKERTLKEWDFVLKEAGFARYEVRDIDDVQ 380

BLAST of Lag0001384 vs. TAIR 10
Match: AT4G35150.1 (O-methyltransferase family protein )

HSP 1 Score: 196.8 bits (499), Expect = 3.0e-50
Identity = 123/360 (34.17%), Postives = 185/360 (51.39%), Query Frame = 0

Query: 14  DELLDAQTHIWNHFLSFINSMSLKCAVQLGIADAIHRH-GPHPMPLSQLVSALKIHPQKS 73
           DE   A   IW +   F +  + KCA+ L I +AI  H    P+ LS+L SA+   P   
Sbjct: 10  DEEAKASLDIWRYVFGFADIAAAKCAIDLKIPEAIENHPSSQPVTLSELSSAVSASP--- 69

Query: 74  QFIYRLMRILTHSGFFLTQKVTTKEKQEEGYMLTNSSRLLLKHNPLAVTPFLLSMLDAPL 133
             + R+MR L H G F  ++V TK+    GY  T  SR                      
Sbjct: 70  SHLRRIMRFLVHQGLF--KEVPTKDGLATGYTNTPLSR---------------------- 129

Query: 134 VEPWQFLSVWFRSEDRTPYDTAHGTSFWEYMMDHNKSKDGEVFNAGMASDARLVMSVLLG 193
                          R      HG   W +  D+      ++ N  MA DAR V+  + G
Sbjct: 130 ---------------RMMITKLHGKDLWAFAQDN--LCHSQLINEAMACDARRVVPRVAG 189

Query: 194 KYRGVFEGLGSLVDVGGGTGTMAKAIAEAFPEIECIVFDLPQVVAGLKGKGNLKYVEGDM 253
             +G+F+G+ ++VDVGGGTG     + + FP I+   FDLP V+   +    ++ VEGDM
Sbjct: 190 ACQGLFDGVATVVDVGGGTGETMGILVKEFPWIKGFNFDLPHVIEVAQVLDGVENVEGDM 249

Query: 254 FKAIPPADALLLKWILHDWSDEECVKILKKCKTAITSKCNKGKVIVIDMVVGNQK----- 313
           F +IP +DA+++KW+LHDW D++C+KILK CK A+    N GKV++++ V+G +K     
Sbjct: 250 FDSIPASDAVIIKWVLHDWGDKDCIKILKNCKEAVLP--NIGKVLIVECVIGEKKNTMIA 309

Query: 314 --KDDSIE-TQLLFDMLMMILVS-GREREEKEWAQLIKKAGFSGYKIFPIMGLRSLIEIY 364
             +DD +E  +L  DM+MM+  S G+ER  KEW  ++ +AGF+ Y++     ++SLI  Y
Sbjct: 310 EERDDKLEHVRLQLDMVMMVHTSTGKERTLKEWDFVLTEAGFARYEVRDFDDVQSLIIAY 323

BLAST of Lag0001384 vs. TAIR 10
Match: AT5G54160.1 (O-methyltransferase 1 )

HSP 1 Score: 162.9 bits (411), Expect = 4.7e-40
Identity = 107/323 (33.13%), Postives = 177/323 (54.80%), Query Frame = 0

Query: 34  MSLKCAVQLGIADAIHRHGPHPMPLSQLVSALKI-HPQKSQFIYRLMRILT-HSGFFLTQ 93
           M+LK A++L + + + ++G  PM  +++ S L   +P+    + R++R+LT +S    + 
Sbjct: 34  MALKSALELDLLEIMAKNG-SPMSPTEIASKLPTKNPEAPVMLDRILRLLTSYSVLTCSN 93

Query: 94  KVTTKEKQEEGYMLTNSSRLLLKH-NPLAVTPFLLSMLDAPLVEPWQFLSVWFRSEDRTP 153
           +  + +  E  Y L    + L K+ + +++    L   D  L+E W  L      +   P
Sbjct: 94  RKLSGDGVERIYGLGPVCKYLTKNEDGVSIAALCLMNQDKVLMESWYHLKDAI-LDGGIP 153

Query: 154 YDTAHGTSFWEYMMDHNKSKDGEVFNAGMASDARLVMSVLLGKYRGVFEGLGSLVDVGGG 213
           ++ A+G S +EY       +  +VFN GM++ + + M  +L  Y+G FEGL SLVDVGGG
Sbjct: 154 FNKAYGMSAFEY--HGTDPRFNKVFNNGMSNHSTITMKKILETYKG-FEGLTSLVDVGGG 213

Query: 214 TGTMAKAIAEAFPEIECIVFDLPQVVAGLKGKGNLKYVEGDMFKAIPPADALLLKWILHD 273
            G   K I   +P ++ I FDLP V+        +++V GDMF ++P  DA+ +KWI HD
Sbjct: 214 IGATLKMIVSKYPNLKGINFDLPHVIEDAPSHPGIEHVGGDMFVSVPKGDAIFMKWICHD 273

Query: 274 WSDEECVKILKKCKTAITSKCNKGKVIVIDMVVGNQKKDDSIETQLLFDMLMMILV---S 333
           WSDE CVK LK C  ++      GKVI+ + ++  +  D S+ T+ +  +  ++L     
Sbjct: 274 WSDEHCVKFLKNCYESLP---EDGKVILAECIL-PETPDSSLSTKQVVHVDCIMLAHNPG 333

Query: 334 GREREEKEWAQLIKKAGFSGYKI 351
           G+ER EKE+  L K +GF G K+
Sbjct: 334 GKERTEKEFEALAKASGFKGIKV 347

BLAST of Lag0001384 vs. TAIR 10
Match: AT1G51990.1 (O-methyltransferase family protein )

HSP 1 Score: 161.0 bits (406), Expect = 1.8e-39
Identity = 117/366 (31.97%), Postives = 187/366 (51.09%), Query Frame = 0

Query: 9   TIGASDELLDAQTHIWNHFLSFINSMSLKCAVQLGIADAIHRH---GPHPMPLSQLVSAL 68
           T G S E  D    I    L+F+  + +K A +L + + + +    G +  P+     A 
Sbjct: 6   TSGGSSEEEDMLLAIQLGGLNFVPYI-VKTARELDLFEIMAKARPLGSYLSPVDLASMAA 65

Query: 69  KIHPQKSQFIYRLMRILTHSGFFLTQKVTTKE-KQEEGYMLTNSSRLLLK-HNPLAVTPF 128
             +P     I RL+R L        + V  +E ++   Y L    + L+K  +  ++ P+
Sbjct: 66  PKNPHAPMMIDRLLRFLVAYSVCTCKLVKDEEGRESRAYGLGKVGKKLIKDEDGFSIAPY 125

Query: 129 LLSMLDAPLVEPWQFLSVWFRSEDRTPYDTAHGTSFWEYMMDHNKSKDGEVFNAGMASDA 188
           +L+         W +L+   +    + ++ A+    +EYM  +   K  ++FN  M +  
Sbjct: 126 VLAGCTKAKGGVWSYLTEAIQEGGASAWERANEALIFEYMKKNENLK--KIFNESMTNHT 185

Query: 189 RLVMSVLLGKYRGVFEGLGSLVDVGGGTGTMAKAIAEAFPEIECIVFDLPQVVAGLKGKG 248
            +VM  +L  Y G FEG+   VDVGG  G+    I   +P I+ I FDLP +V       
Sbjct: 186 SIVMKKILENYIG-FEGVSDFVDVGGSLGSNLAQILSKYPHIKGINFDLPHIVKEAPQIH 245

Query: 249 NLKYVEGDMFKAIPPADALLLKWILHDWSDEECVKILKKCKTAITSKCNKGKVIVIDMVV 308
            ++++ GDMF  IP  + +L+KWILHDW+DE+CV+ILK CK A+      G++IVI+M+V
Sbjct: 246 GVEHIGGDMFDEIPRGEVILMKWILHDWNDEKCVEILKNCKKALP---ETGRIIVIEMIV 305

Query: 309 GNQKKDDSIETQ--LLFDMLMMILVS-GREREEKEWAQLIKKAGFSGYKIFPIMGLRS-- 365
             +  +  + T+  L  D+ MM L S G+ER +KE+  L K+AGF   KI  I G  S  
Sbjct: 306 PREVSETDLATKNSLSADLTMMSLTSGGKERTKKEFEDLAKEAGFKLPKI--IYGAYSYW 362

BLAST of Lag0001384 vs. TAIR 10
Match: AT1G51990.2 (O-methyltransferase family protein )

HSP 1 Score: 156.4 bits (394), Expect = 4.4e-38
Identity = 116/366 (31.69%), Postives = 185/366 (50.55%), Query Frame = 0

Query: 9   TIGASDELLDAQTHIWNHFLSFINSMSLKCAVQLGIADAIHRH---GPHPMPLSQLVSAL 68
           T G S E  D    I    L+F+  + +K A +L + + + +    G +  P+     A 
Sbjct: 6   TSGGSSEEEDMLLAIQLGGLNFVPYI-VKTARELDLFEIMAKARPLGSYLSPVDLASMAA 65

Query: 69  KIHPQKSQFIYRLMRILTHSGFFLTQKVTTKE-KQEEGYMLTNSSRLLLK-HNPLAVTPF 128
             +P     I RL+R L        + V  +E ++   Y L    + L+K  +  ++ P+
Sbjct: 66  PKNPHAPMMIDRLLRFLVAYSVCTCKLVKDEEGRESRAYGLGKVGKKLIKDEDGFSIAPY 125

Query: 129 LLSMLDAPLVEPWQFLSVWFRSEDRTPYDTAHGTSFWEYMMDHNKSKDGEVFNAGMASDA 188
           +L+         W  +    +    + ++ A+    +EYM  +   K  ++FN  M +  
Sbjct: 126 VLAGCTKAKGGVWYNVQHAIQEGGASAWERANEALIFEYMKKNENLK--KIFNESMTNHT 185

Query: 189 RLVMSVLLGKYRGVFEGLGSLVDVGGGTGTMAKAIAEAFPEIECIVFDLPQVVAGLKGKG 248
            +VM  +L  Y G FEG+   VDVGG  G+    I   +P I+ I FDLP +V       
Sbjct: 186 SIVMKKILENYIG-FEGVSDFVDVGGSLGSNLAQILSKYPHIKGINFDLPHIVKEAPQIH 245

Query: 249 NLKYVEGDMFKAIPPADALLLKWILHDWSDEECVKILKKCKTAITSKCNKGKVIVIDMVV 308
            ++++ GDMF  IP  + +L+KWILHDW+DE+CV+ILK CK A+      G++IVI+M+V
Sbjct: 246 GVEHIGGDMFDEIPRGEVILMKWILHDWNDEKCVEILKNCKKALP---ETGRIIVIEMIV 305

Query: 309 GNQKKDDSIETQ--LLFDMLMMILVS-GREREEKEWAQLIKKAGFSGYKIFPIMGLRS-- 365
             +  +  + T+  L  D+ MM L S G+ER +KE+  L K+AGF   KI  I G  S  
Sbjct: 306 PREVSETDLATKNSLSADLTMMSLTSGGKERTKKEFEDLAKEAGFKLPKI--IYGAYSYW 362