Lag0001373 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0001373
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	DDB1- and CUL4-associated factor 8
Location	chr4: 30343266 .. 30347747 (+)
RNA-Seq Expression	Lag0001373
Synteny	Lag0001373

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAACAAGAGGGCCAGAATTAGTCATTCCAATAAGGCTGTCGTCAATGTCTGGAAGCGGGAGGTCGGCGAGCTCTCCTCTAGAGATTTCGCCCACCGTCTTGGTGCCTCGGAGGTGTTGTTTATCCTTCTCTGATTGTCTATCTTGATTTCTGCTTTACCGGTTATTTTTTGTATGCCCAAATAATCTTGTCAGGTTGTAGAGTTGTTCTGATTGTCAAATGTTGTCGTTTGTCGTTTGATCAGAACTGGATAAACCAAGCGGAATGAATCTAGTTGTGTTGCATTATAAAAGGGTTTTATTTTGATTGAGATTATACAGTTTTTGGTGGATAGTTTACTAGCTCAAATAGTTGGAGTATTCGTGTTGATATTTATACTGTTTCAAAGTTGTGTTAGTATTCGTGTTGTTTCCGCAGCTGAGAATCTATGGACCCTTTTGATTGTTGAACCTGCAGGATTAATCGTTGTTGTTATTACTGTAATTTCAGGATCTCGTGCTCCGACTTGATCTTTTCAGGAAATTGGAGAGGCACAAAGGTTGTGTGAATACCGTGAGCTTTAGTGCTGCTGGTGACATTCTAGTGTCAGGCTCTGATGATAGGCGGGTGATCCTCTGGAATTGGGAAACCGGGCATGCCAAACTCTGTTTTCAGTCTGGTCATAATAATAATGTTTTCCAAGCAAAAATCATGCCTTACACGGATGACAATAGCATTGTGACTTGTGCTGCGGATGGGCAGGTATATTATCTAGTTGAAGGGCTGATTTTTTCTAGACCGTACAACATGGAATTAGACCCTTGTGGTCTAACATGGTTCTTGTTTTAGTACTCATGTCAAGAACTCTTACTTTCTCTCTCAATATAGGTATGCTGTACATTTAGATTTAGCTAAAATACTATAGTTTCTCTCTAGCCTAATGGATCATAATGGTGCTTCGATTGCAACTTTCCACAATTATTTAGCTTTCCTTTTGAGCCACCTTCTGAGGTGATTCTAGGAGCCAATTTGCTGTGTCATTAGGGAAACAACAGTTTAGTCCAAAGGTTTTCAAAAGGGATAACCGCCTAGCCACAAACTCACACTGAACGAATTGGAGTTCCGTAGTCTCTTCATCCTTTTTTACATTTTGGGGAACATCTGAGGCTGAGGCTGGTTATGGAGGAAGCAACTTCTTTGAATTCTATCTTTGATTGTTTTGATGTCTGTTTTACACATCTTTTAAATCCACTTTCAAATTTTCATGCTAAAACTGTCTAATATTGACCAACCTTTATTGAGGCCTTTGACGAGACACTGGCTTTGTCAGGGTGATAAAGATTGATGACAGAAGTTCCTTTGCAGCTGGCTTTGTATATTATCAAGAATTCTTCCCTTGCTATCGTGGAAGCAACATCTTGTGATGACTATCACTAGAGGCTCTCCTTTCCTAGAATATTTGGCTGTTGTTCATTCAAGCGGGAGATTGTCAGTCTTTCACTACATCTGGCTGCTTATGCCAAATATCTCTCCTTCCTGATAAAGTTTTCCTTCACCGGAGCTGTTTATTCTACCCCTCAAAGAATCTTTTGCTCAGCAAAAGAAACTGAATAATTTTCTCCCTCTGGAATCACTAAACTCAATCTCTTTCCACTACTTCGGATGTGAGAAGTCTTTATGAAATCCCATATTGTTGAAATTTTCTTGATCCAAATGAAGCGATTTCCACACTCAGTCTTCTAAAATAATCCTACATAAAGTGGTGTTAAAAGGAGGTTCTCCACAATCAAATTGACTTTCTTTGTTCCACTTGAAAATACTTGCCTCTCCATTATCCGTCAAACTCGACCTATAACCATTTCTTTTTGATGCCTTGTCCTTATGGCTTTAGGTGGTTTTATTCTTGATGCTCTTCAACAACAACAGCAACTGTGTTGTCTACTGACATTGATTTATTAATTCATGTTCTGAAAAAGAATTTCTCTTTTTCTCAATTTAAGTAAAACTTCCTGTTTATTTTCTGTCAGCGGAATATTTACTTTGTTTTCTCTTATCAACAATAGTTTTGTTGTTTACTAACATTCATTTATTAAATTATAATTTAAAAAAGAATTCTGTCCTTCTCAATTTAAGTTAAACGGCCTGTTTATTTTCTGTGAGCAGAATATTTACTTTGTTTTCTCCCGCTGTAAAACTTGTCTATTTGTGCTCTGTGTTAAAACATTCATTTCTTGAAGAGTATTATTTGCTGAAATTCAGGTGAGACATGCTCAGATTCTGGATGGTGGGGATGTGGAGACTGCATTACTTGGCAGTCATCAAGGGCGTGCTCATAAGTTGGCCATTGAACCTGGGAGCCCTTATATCTTTTATACTTGTGGTGAAGATGGATTGGTTCAACATGTGAGTTTGGTTGTTCATTCTGTTGTTATATTATGTTCCACCAACATTCCTAGTACTATTACAACTCAATAAGTTCAATACTTATTCACAGCCAAAGAACTCTACACGTTTTAAAGATTTATTACTGAACCTAAAGAATGAAGTGAGATGTTTTCTAAAGACCATTTATCTTTAATATCTTTGAATTGCAATGTGATACTGCCCACTTTACTTTTTTAACAGTTTGATCTAAGAACTAGGGCTGCCATTGAGCTGTTCACTTGCCAGTCAGTTGACAAGAGGGCAGGTTACATGTCATCGATTCAGCTAAATGCAATTGCTATTGATCCCAGAAATCCAAATCTCTTTGTCGTTGCTGGTTCAGATGAGTATGCACGGTTGTATGATATTCGCAAATATAAGGGGGAAGGATCAACTGATTTTGGTCAACCTGCAGACTGCTATTGCCCCCCTCACTTGATTGGTGATGAACAAGTGGGAATAACAGGTTTAGCCTTCTCAGAGCTGAGTGAACTCCTTGTTTCATACAATGATGAATCTATTTATCTCTTTAATAGGGATATGGGTTTGGGCCGAAGCCCATATCCATGGTCTCCAGATACAAGTGATATGGGAGCTGAAAAAGTTAAGGATGGTAGTCCACAAGTTTATAAAGGACATAGAAACTGTGAGACAGTAAAAGGTGTCAATTTTTTTGGACCTAAATGTGAGTATGTCATCAGTGGATCTGATTGTGGCAGAATATTTATATGGAGGAAAAAGGGTGGAGGGCTAATCCGAGTCATGGAAGCAGACGAGGATGTGGTGAACTGCATTGAGCCCCATCCTCACATGACCATGCTTGCTAGTAGTGGAATTGAAAGTGACGTCAAGTTATGGACTCCAAAGGCCCTTGAAAGGGCTACTCTGCCTGAGAAAATTGAACAGGTAGGAATGTCATCTCCATTCTTCAGTTTAATGCCAATAGTGATGCTTTACTATAGCCTATTGAATGGCATTTGACTCTTTGAATTATTGAGGTTTCTTTTGTATAAATTGAATAAAACATCCTATGTCCATTCCAGCCATAAGGATAATAGTACCGGCCATTTCATATTCTTGACTCTCCTATATTTATTCAAACATGATCATATTTCCTCTATTTTTTATCAGTCTGTGTTACTGTCTTCTGCTTGTATGGATGGATGAGTTTGTGTCGCTCTTTTCCCCCTTGTTTTTCTGCATGTAGACTTGTAGAGTCATAAGAATCAGAATGAACGTGTCCTATAAATTTGTCATTCTATATCGCCATTTTTTTTTTTTGAGAATATCTATATCACCAATTGAAGTTATCTTGTATGATGACCTTAATAACATTTTGGGGTGTTTATTTTTAACAAATCATTTGTGTTTTTTCAAAAACTATGTTCTTTGGAACAACAAGGTGGGATGCTGAGTATGTTTCTAGTACATTTCTTGACCAATTAATGCCATATGAACATGATATGGATATGTGGTAGTCTTGTTCAATGAAAAGAAAGAAAGAAACCACGAGGCAATTGAGATAGACTCATAAGTAAGCTATAGTTATCCAGATTCCACAGAGAATCTTTGGTAACTGATGTCAACATTTTATATGTGAACAGAATTATGGCTAATACACATATTCATATACATTCTGTAGATGACATGAGCAGAGGTTTATTTCTCTGGTGTTTATAAATGACCTGGTTCAATTCCTTCATTTGGTGAAGAACATAGGTTTTGTGATGATTGGTATTGTTTTCAATGATGATTACAAAGATGATGAAGACAATGAAGGCAACGATGATAAGGGGAAAGTATGACAGCGATGTTCATAAATATCATTCTTCTGATCTAATGATTTTGAACTGTGCATTTTGCAGAAACTGAAGCCTAGGGGATGGATGCACCGGTTAGCTTTGCCGGAGGAACTGATGATGCAACTGTTTTCACTGCGAAGACAGAATTCCAGCCCCGAACACCGAAGAGAAAACTCCGCTGTAGGTAGGGAAATTTTAGAGTTGATAATGACATTCAATAACAACACCGAGAGCTCTTCAGAAGACAACGGTGGTGATACTGCTGAAGATGATTGA

mRNA sequence

ATGAACAAGAGGGCCAGAATTAGTCATTCCAATAAGGCTGTCGTCAATGTCTGGAAGCGGGAGGTCGGCGAGCTCTCCTCTAGAGATTTCGCCCACCGTCTTGGTGCCTCGGAGGATCTCGTGCTCCGACTTGATCTTTTCAGGAAATTGGAGAGGCACAAAGGTTGTGTGAATACCGTGAGCTTTAGTGCTGCTGGTGACATTCTAGTGTCAGGCTCTGATGATAGGCGGGTGATCCTCTGGAATTGGGAAACCGGGCATGCCAAACTCTGTTTTCAGTCTGGTCATAATAATAATGTTTTCCAAGCAAAAATCATGCCTTACACGGATGACAATAGCATTGTGACTTGTGCTGCGGATGGGCAGGTGAGACATGCTCAGATTCTGGATGGTGGGGATGTGGAGACTGCATTACTTGGCAGTCATCAAGGGCGTGCTCATAAGTTGGCCATTGAACCTGGGAGCCCTTATATCTTTTATACTTGTGGTGAAGATGGATTGGTTCAACATTTTGATCTAAGAACTAGGGCTGCCATTGAGCTGTTCACTTGCCAGTCAGTTGACAAGAGGGCAGGTTACATGTCATCGATTCAGCTAAATGCAATTGCTATTGATCCCAGAAATCCAAATCTCTTTGTCGTTGCTGGTTCAGATGAGTATGCACGGTTGTATGATATTCGCAAATATAAGGGGGAAGGATCAACTGATTTTGGTCAACCTGCAGACTGCTATTGCCCCCCTCACTTGATTGGTGATGAACAAGTGGGAATAACAGGTTTAGCCTTCTCAGAGCTGAGTGAACTCCTTGTTTCATACAATGATGAATCTATTTATCTCTTTAATAGGGATATGGGTTTGGGCCGAAGCCCATATCCATGGTCTCCAGATACAAGTGATATGGGAGCTGAAAAAGTTAAGGATGGTAGTCCACAAGTTTATAAAGGACATAGAAACTGTGAGACAGTAAAAGGTGTCAATTTTTTTGGACCTAAATGTGAGTATGTCATCAGTGGATCTGATTGTGGCAGAATATTTATATGGAGGAAAAAGGGTGGAGGGCTAATCCGAGTCATGGAAGCAGACGAGGATGTGGTGAACTGCATTGAGCCCCATCCTCACATGACCATGCTTGCTAGTAGTGGAATTGAAAGTGACGTCAAGTTATGGACTCCAAAGGCCCTTGAAAGGGCTACTCTGCCTGAGAAAATTGAACAGAAACTGAAGCCTAGGGGATGGATGCACCGGTTAGCTTTGCCGGAGGAACTGATGATGCAACTGTTTTCACTGCGAAGACAGAATTCCAGCCCCGAACACCGAAGAGAAAACTCCGCTGTAGGTAGGGAAATTTTAGAGTTGATAATGACATTCAATAACAACACCGAGAGCTCTTCAGAAGACAACGGTGGTGATACTGCTGAAGATGATTGA

Coding sequence (CDS)

ATGAACAAGAGGGCCAGAATTAGTCATTCCAATAAGGCTGTCGTCAATGTCTGGAAGCGGGAGGTCGGCGAGCTCTCCTCTAGAGATTTCGCCCACCGTCTTGGTGCCTCGGAGGATCTCGTGCTCCGACTTGATCTTTTCAGGAAATTGGAGAGGCACAAAGGTTGTGTGAATACCGTGAGCTTTAGTGCTGCTGGTGACATTCTAGTGTCAGGCTCTGATGATAGGCGGGTGATCCTCTGGAATTGGGAAACCGGGCATGCCAAACTCTGTTTTCAGTCTGGTCATAATAATAATGTTTTCCAAGCAAAAATCATGCCTTACACGGATGACAATAGCATTGTGACTTGTGCTGCGGATGGGCAGGTGAGACATGCTCAGATTCTGGATGGTGGGGATGTGGAGACTGCATTACTTGGCAGTCATCAAGGGCGTGCTCATAAGTTGGCCATTGAACCTGGGAGCCCTTATATCTTTTATACTTGTGGTGAAGATGGATTGGTTCAACATTTTGATCTAAGAACTAGGGCTGCCATTGAGCTGTTCACTTGCCAGTCAGTTGACAAGAGGGCAGGTTACATGTCATCGATTCAGCTAAATGCAATTGCTATTGATCCCAGAAATCCAAATCTCTTTGTCGTTGCTGGTTCAGATGAGTATGCACGGTTGTATGATATTCGCAAATATAAGGGGGAAGGATCAACTGATTTTGGTCAACCTGCAGACTGCTATTGCCCCCCTCACTTGATTGGTGATGAACAAGTGGGAATAACAGGTTTAGCCTTCTCAGAGCTGAGTGAACTCCTTGTTTCATACAATGATGAATCTATTTATCTCTTTAATAGGGATATGGGTTTGGGCCGAAGCCCATATCCATGGTCTCCAGATACAAGTGATATGGGAGCTGAAAAAGTTAAGGATGGTAGTCCACAAGTTTATAAAGGACATAGAAACTGTGAGACAGTAAAAGGTGTCAATTTTTTTGGACCTAAATGTGAGTATGTCATCAGTGGATCTGATTGTGGCAGAATATTTATATGGAGGAAAAAGGGTGGAGGGCTAATCCGAGTCATGGAAGCAGACGAGGATGTGGTGAACTGCATTGAGCCCCATCCTCACATGACCATGCTTGCTAGTAGTGGAATTGAAAGTGACGTCAAGTTATGGACTCCAAAGGCCCTTGAAAGGGCTACTCTGCCTGAGAAAATTGAACAGAAACTGAAGCCTAGGGGATGGATGCACCGGTTAGCTTTGCCGGAGGAACTGATGATGCAACTGTTTTCACTGCGAAGACAGAATTCCAGCCCCGAACACCGAAGAGAAAACTCCGCTGTAGGTAGGGAAATTTTAGAGTTGATAATGACATTCAATAACAACACCGAGAGCTCTTCAGAAGACAACGGTGGTGATACTGCTGAAGATGATTGA

Protein sequence

MNKRARISHSNKAVVNVWKREVGELSSRDFAHRLGASEDLVLRLDLFRKLERHKGCVNTVSFSAAGDILVSGSDDRRVILWNWETGHAKLCFQSGHNNNVFQAKIMPYTDDNSIVTCAADGQVRHAQILDGGDVETALLGSHQGRAHKLAIEPGSPYIFYTCGEDGLVQHFDLRTRAAIELFTCQSVDKRAGYMSSIQLNAIAIDPRNPNLFVVAGSDEYARLYDIRKYKGEGSTDFGQPADCYCPPHLIGDEQVGITGLAFSELSELLVSYNDESIYLFNRDMGLGRSPYPWSPDTSDMGAEKVKDGSPQVYKGHRNCETVKGVNFFGPKCEYVISGSDCGRIFIWRKKGGGLIRVMEADEDVVNCIEPHPHMTMLASSGIESDVKLWTPKALERATLPEKIEQKLKPRGWMHRLALPEELMMQLFSLRRQNSSPEHRRENSAVGREILELIMTFNNNTESSSEDNGGDTAEDD

Homology

[Close]

BLAST of Lag0001373 vs. NCBI nr
Match: XP_022136068.1 (DDB1- and CUL4-associated factor 8 [Momordica charantia])

HSP 1 Score: 876.7 bits (2264), Expect = 9.0e-251
Identity = 422/480 (87.92%), Postives = 448/480 (93.33%), Query Frame = 0


Query: 1   MNKRARISHSNKAVVNVWKREVGELSSRDFAHRLGASEDLVLRLDLFRKLERHKGCVNTV 60
           MNKRARI  SNKA+VNVWKREVGELS+R+FAHRLGAS+DLVLRLD++RKLERHKGCVNTV
Sbjct: 1   MNKRARIDDSNKALVNVWKREVGELSTRNFAHRLGASQDLVLRLDIYRKLERHKGCVNTV 60

Query: 61  SFSAAGDILVSGSDDRRVILWNWETGHAKLCFQSGHNNNVFQAKIMPYTDDNSIVTCAAD 120
           SF+AAGD+LVSGSDDRRVILWNWETG  KL FQSGHNNNVFQAKIMPYTDD  IVTCAAD
Sbjct: 61  SFNAAGDVLVSGSDDRRVILWNWETGQVKLSFQSGHNNNVFQAKIMPYTDDRCIVTCAAD 120

Query: 121 GQVRHAQILDGGDVETALLGSHQGRAHKLAIEPGSPYIFYTCGEDGLVQHFDLRTRAAIE 180
           GQVRHAQIL+ G VET+LLGSHQGRAHKLAIEPGSPYIFYTCGEDGLVQHFDLRT AA E
Sbjct: 121 GQVRHAQILNSGRVETSLLGSHQGRAHKLAIEPGSPYIFYTCGEDGLVQHFDLRTGAASE 180

Query: 181 LFTCQSVDKRAGYMSSIQLNAIAIDPRNPNLFVVAGSDEYARLYDIRKYKGEGSTDFGQP 240
           LFTCQS+D RAGYMSSIQLNAI IDPRNPNLFVVAGSDEYARLYDIRKYKGEGSTDFGQP
Sbjct: 181 LFTCQSIDNRAGYMSSIQLNAITIDPRNPNLFVVAGSDEYARLYDIRKYKGEGSTDFGQP 240

Query: 241 ADCYCPPHLIGDEQVGITGLAFSELSELLVSYNDESIYLFNRDMGLGRSPYPWSP----- 300
            D YCPPHLIGDEQVGITGLA+SELSELLVSYNDESIYLFNRDMGLG +P P SP     
Sbjct: 241 TDYYCPPHLIGDEQVGITGLAYSELSELLVSYNDESIYLFNRDMGLGPNPNPVSPVSMSS 300

Query: 301 DTSDMGAEKVKDGSPQVYKGHRNCETVKGVNFFGPKCEYVISGSDCGRIFIWRKKGGGLI 360
           D+S+MGAE V+ GSPQVYKGHRNCETVKGVNFFGPKCEYV+SGSDCGRIFIW+KKGG LI
Sbjct: 301 DSSEMGAENVEHGSPQVYKGHRNCETVKGVNFFGPKCEYVVSGSDCGRIFIWKKKGGELI 360

Query: 361 RVMEADEDVVNCIEPHPHMTMLASSGIESDVKLWTPKALERATLPEKIEQKLKPRGWMHR 420
           RVMEADEDVVNCIEPHPH+TMLASSGIESD+K+WTPKALERATLPEKIEQK KPRGWMHR
Sbjct: 361 RVMEADEDVVNCIEPHPHVTMLASSGIESDIKIWTPKALERATLPEKIEQKPKPRGWMHR 420

Query: 421 LALPEELMMQLFSLRRQNSSPEHRRENSAVGREILELIMTFNNNTESSSEDNGGDTAEDD 476
           LALPEELMMQLFSLRR+NSSPE RRENSAV REILELI+TFN N++ SS+DNG DTAEDD
Sbjct: 421 LALPEELMMQLFSLRRRNSSPERRRENSAVSREILELILTFNANSDESSDDNGDDTAEDD 480

[Close]

BLAST of Lag0001373 vs. NCBI nr
Match: XP_038887987.1 (DDB1- and CUL4-associated factor 8-like [Benincasa hispida])

HSP 1 Score: 846.7 bits (2186), Expect = 1.0e-241
Identity = 418/481 (86.90%), Postives = 436/481 (90.64%), Query Frame = 0


Query: 1   MNKRARISHSNKAVVNVWKREVGELSSRDFAHRLGASEDLVLRLDLFRKLERHKGCVNTV 60
           MNKRARI  SNKAVVNVWKREVGELS+R+FAHRLGA EDLVLRLDL+RKLERHKGCVNTV
Sbjct: 1   MNKRARIDDSNKAVVNVWKREVGELSTRNFAHRLGALEDLVLRLDLYRKLERHKGCVNTV 60

Query: 61  SFSAAGDILVSGSDDRRVILWNWETGHAKLCFQSGHNNNVFQAKIMPYTDDNSIVTCAAD 120
           SF+AAGDILVSGSDDRRVILWNWETGH KL FQSGHNNNVFQAKIMPYTDD SIVTCAAD
Sbjct: 61  SFNAAGDILVSGSDDRRVILWNWETGHVKLSFQSGHNNNVFQAKIMPYTDDRSIVTCAAD 120

Query: 121 GQVRHAQILDGGDVETALLGSHQGRAHKLAIEPGSPYIFYTCGEDGLVQHFDLRTRAAIE 180
           GQVRHAQIL+ G VETALLGSH GRAHKLA EPGSPY+FYTCGEDGLVQ FDLRT AA+E
Sbjct: 121 GQVRHAQILNSGRVETALLGSHLGRAHKLAFEPGSPYMFYTCGEDGLVQRFDLRTGAAVE 180

Query: 181 LFTCQSVDKRAGYMSSIQLNAIAIDPRNPNLFVVAGSDEYARLYDIRKYKGEGSTDFGQP 240
           LFTCQSVD RAGYMSSIQLNAIAIDPRNPNLFVVAGSDEYARLYDIRK   +GSTDFGQ 
Sbjct: 181 LFTCQSVDNRAGYMSSIQLNAIAIDPRNPNLFVVAGSDEYARLYDIRKSGEDGSTDFGQL 240

Query: 241 ADCYCPPHLIGDEQVGITGLAFSELSELLVSYNDESIYLFNRDMGLGRSPYPWSP----- 300
            DCYCPPHLIGDEQVGITGLAFSE+SELLVSYNDESIYLFNRDMGLG +P P SP     
Sbjct: 241 VDCYCPPHLIGDEQVGITGLAFSEMSELLVSYNDESIYLFNRDMGLGPNPNPASPVSLSS 300

Query: 301 DTSDMGAEKVKDGSPQVYKGHRNCETVKGVNFFGPKCEYVISGSDCGRIFIWRKKGGGLI 360
           DTS+M AE V++ SPQVYKGHRNCETVKGVNFFGPKCEYV+SGSDCGRIFIWRKKGG LI
Sbjct: 301 DTSEMEAENVENVSPQVYKGHRNCETVKGVNFFGPKCEYVVSGSDCGRIFIWRKKGGELI 360

Query: 361 RVMEADEDVVNCIEPHPHMTMLASSGIESDVKLWTPKALERATLPEKIEQKLKPRGWMHR 420
           RVMEADEDVVNCIE HPHMTMLASSGIESD+K+WTPKALERATLPEKIEQK KPRGWMHR
Sbjct: 361 RVMEADEDVVNCIESHPHMTMLASSGIESDIKIWTPKALERATLPEKIEQKPKPRGWMHR 420

Query: 421 LALPEELMMQLFSLRRQNSSPEHRRENSAVGREILELIMTFNN-NTESSSEDNGGDTAED 476
           LALPEELMMQLFSLRRQ  SPE  RE   V REILELIMTFNN N++ SS  NG DTAED
Sbjct: 421 LALPEELMMQLFSLRRQIMSPERGREPPTVSREILELIMTFNNGNSDDSSYYNGDDTAED 480

[Close]

BLAST of Lag0001373 vs. NCBI nr
Match: XP_008447917.1 (PREDICTED: DDB1- and CUL4-associated factor 8 [Cucumis melo])

HSP 1 Score: 845.5 bits (2183), Expect = 2.2e-241
Identity = 414/480 (86.25%), Postives = 430/480 (89.58%), Query Frame = 0


Query: 1   MNKRARISHSNKAVVNVWKREVGELSSRDFAHRLGASEDLVLRLDLFRKLERHKGCVNTV 60
           MNKRARI  SNKAVVNVWKREVGELS+R+FAHRLGA EDLVLRLDL++KLERHKGCVNTV
Sbjct: 1   MNKRARIDDSNKAVVNVWKREVGELSTRNFAHRLGALEDLVLRLDLYKKLERHKGCVNTV 60

Query: 61  SFSAAGDILVSGSDDRRVILWNWETGHAKLCFQSGHNNNVFQAKIMPYTDDNSIVTCAAD 120
           SF+AAGDILVSGSDDRRVILWNWETG  KL FQSGHNNNVFQAKIMPYTDD SIVTCAAD
Sbjct: 61  SFNAAGDILVSGSDDRRVILWNWETGRVKLSFQSGHNNNVFQAKIMPYTDDRSIVTCAAD 120

Query: 121 GQVRHAQILDGGDVETALLGSHQGRAHKLAIEPGSPYIFYTCGEDGLVQHFDLRTRAAIE 180
           GQVRHAQIL+ G VET+LLGSH GRAHKLAIEPGSPY+FYTCGEDGLVQ FDLRT AA+E
Sbjct: 121 GQVRHAQILNSGHVETSLLGSHLGRAHKLAIEPGSPYMFYTCGEDGLVQRFDLRTGAAVE 180

Query: 181 LFTCQSVDKRAGYMSSIQLNAIAIDPRNPNLFVVAGSDEYARLYDIRKYKGEGSTDFGQP 240
           LFTCQSVD RAGYMSSIQLNAI IDPRNPNLFVVAGSDEYARLYDIRK   +GSTDFGQ 
Sbjct: 181 LFTCQSVDNRAGYMSSIQLNAIVIDPRNPNLFVVAGSDEYARLYDIRKSGEDGSTDFGQL 240

Query: 241 ADCYCPPHLIGDEQVGITGLAFSELSELLVSYNDESIYLFNRDMGLGRSPYPWSP----- 300
           ADCYCPPHLIGDEQVGITGLAFSELSELLVSYNDESIYLFNRDMGLG +P P  P     
Sbjct: 241 ADCYCPPHLIGDEQVGITGLAFSELSELLVSYNDESIYLFNRDMGLGPNPNPAPPLSLSS 300

Query: 301 DTSDMGAEKVKDGSPQVYKGHRNCETVKGVNFFGPKCEYVISGSDCGRIFIWRKKGGGLI 360
           D S+MGAEKV +G PQVYKGHRNCETVKGVNFFGPKCEYV+SGSDCGRIFIWRKKGG LI
Sbjct: 301 DASEMGAEKVDNGIPQVYKGHRNCETVKGVNFFGPKCEYVVSGSDCGRIFIWRKKGGELI 360

Query: 361 RVMEADEDVVNCIEPHPHMTMLASSGIESDVKLWTPKALERATLPEKIEQKLKPRGWMHR 420
           RVMEADEDVVNCIEPHPHMTMLASSGIESDVK+WTPKA ERATLPEKIEQK KPRGWMHR
Sbjct: 361 RVMEADEDVVNCIEPHPHMTMLASSGIESDVKIWTPKAHERATLPEKIEQKPKPRGWMHR 420

Query: 421 LALPEELMMQLFSLRRQNSSPEHRRENSAVGREILELIMTFNNNTESSSEDNGGDTAEDD 476
           LALPEELMMQLFSLRRQ SSPE  RE   V RE+LELIMTFNN     S D   DT EDD
Sbjct: 421 LALPEELMMQLFSLRRQTSSPERGREPPTVSRELLELIMTFNNGNSDDSSDYNDDTGEDD 480

[Close]

BLAST of Lag0001373 vs. NCBI nr
Match: TYK23393.1 (DDB1- and CUL4-associated factor 8 [Cucumis melo var. makuwa])

HSP 1 Score: 840.1 bits (2169), Expect = 9.4e-240
Identity = 412/480 (85.83%), Postives = 428/480 (89.17%), Query Frame = 0


Query: 1   MNKRARISHSNKAVVNVWKREVGELSSRDFAHRLGASEDLVLRLDLFRKLERHKGCVNTV 60
           MNKRARI  SNKAVVNVWKREVGELS+R+FAHRLGA EDLVLRLDL++KLERHKGCVNTV
Sbjct: 1   MNKRARIDDSNKAVVNVWKREVGELSTRNFAHRLGALEDLVLRLDLYKKLERHKGCVNTV 60

Query: 61  SFSAAGDILVSGSDDRRVILWNWETGHAKLCFQSGHNNNVFQAKIMPYTDDNSIVTCAAD 120
           SF+AAGDILVSGSDDRRVILWNWETG  KL FQSGHNNNVFQAKIMPYTDD SIVTCAAD
Sbjct: 61  SFNAAGDILVSGSDDRRVILWNWETGRVKLSFQSGHNNNVFQAKIMPYTDDRSIVTCAAD 120

Query: 121 GQVRHAQILDGGDVETALLGSHQGRAHKLAIEPGSPYIFYTCGEDGLVQHFDLRTRAAIE 180
           GQVRHAQIL+ G VET+LLGSH GRAHKLAIEPGSPY+FYTCGEDGLVQ FDLRT AA+E
Sbjct: 121 GQVRHAQILNSGHVETSLLGSHLGRAHKLAIEPGSPYMFYTCGEDGLVQRFDLRTGAAVE 180

Query: 181 LFTCQSVDKRAGYMSSIQLNAIAIDPRNPNLFVVAGSDEYARLYDIRKYKGEGSTDFGQP 240
           LFTCQSVD RAGYMSSIQLNAI IDPRNPNLFVVAGSDEYARLYDIRK   +GSTDFGQ 
Sbjct: 181 LFTCQSVDNRAGYMSSIQLNAIVIDPRNPNLFVVAGSDEYARLYDIRKSGEDGSTDFGQL 240

Query: 241 ADCYCPPHLIGDEQVGITGLAFSELSELLVSYNDESIYLFNRDMGLGRSPYPWSP----- 300
            D YCPPHLIGDEQVGITGLAFSELSELLVSYNDESIYLFNRDMGLG +P P  P     
Sbjct: 241 VDSYCPPHLIGDEQVGITGLAFSELSELLVSYNDESIYLFNRDMGLGPNPNPAPPLSLSS 300

Query: 301 DTSDMGAEKVKDGSPQVYKGHRNCETVKGVNFFGPKCEYVISGSDCGRIFIWRKKGGGLI 360
           D S+MGAEKV +G PQVYKGHRNCETVKGVNFFGPKCEYV+SGSDCGRIFIWRKKGG LI
Sbjct: 301 DASEMGAEKVDNGIPQVYKGHRNCETVKGVNFFGPKCEYVVSGSDCGRIFIWRKKGGELI 360

Query: 361 RVMEADEDVVNCIEPHPHMTMLASSGIESDVKLWTPKALERATLPEKIEQKLKPRGWMHR 420
           RVMEADEDVVNCIEPHPHMTMLASSGIESDVK+WTPKA ERATLPEKIEQK KPRGWMHR
Sbjct: 361 RVMEADEDVVNCIEPHPHMTMLASSGIESDVKIWTPKAHERATLPEKIEQKPKPRGWMHR 420

Query: 421 LALPEELMMQLFSLRRQNSSPEHRRENSAVGREILELIMTFNNNTESSSEDNGGDTAEDD 476
           LALPEELMMQLFSLRRQ SSPE  RE   V RE+LELIMTFNN     S D   DT EDD
Sbjct: 421 LALPEELMMQLFSLRRQTSSPERGREPPTVSRELLELIMTFNNGNSDDSSDYNDDTGEDD 480

[Close]

BLAST of Lag0001373 vs. NCBI nr
Match: XP_004144864.1 (DDB1- and CUL4-associated factor 8 [Cucumis sativus] >KGN43277.1 hypothetical protein Csa_020173 [Cucumis sativus])

HSP 1 Score: 836.3 bits (2159), Expect = 1.4e-238
Identity = 409/480 (85.21%), Postives = 427/480 (88.96%), Query Frame = 0


Query: 1   MNKRARISHSNKAVVNVWKREVGELSSRDFAHRLGASEDLVLRLDLFRKLERHKGCVNTV 60
           MNKRARI  SNKAVVNVWKREVGELS+R+FAHRLGA EDLVLRLDL++KLERHKGCVNTV
Sbjct: 1   MNKRARIDDSNKAVVNVWKREVGELSTRNFAHRLGALEDLVLRLDLYKKLERHKGCVNTV 60

Query: 61  SFSAAGDILVSGSDDRRVILWNWETGHAKLCFQSGHNNNVFQAKIMPYTDDNSIVTCAAD 120
           SF+AAGDILVSGSDDRRVILWNWETG  KL FQSGHNNNVFQAKIMPYTDD SIVTCAAD
Sbjct: 61  SFNAAGDILVSGSDDRRVILWNWETGRVKLSFQSGHNNNVFQAKIMPYTDDQSIVTCAAD 120

Query: 121 GQVRHAQILDGGDVETALLGSHQGRAHKLAIEPGSPYIFYTCGEDGLVQHFDLRTRAAIE 180
           GQVRHAQIL+ G VET+LLG+H GRAHKLAIEPGSPY+FYTCGEDGLVQ FDLRT  A+E
Sbjct: 121 GQVRHAQILNSGHVETSLLGNHLGRAHKLAIEPGSPYMFYTCGEDGLVQRFDLRTGDAVE 180

Query: 181 LFTCQSVDKRAGYMSSIQLNAIAIDPRNPNLFVVAGSDEYARLYDIRKYKGEGSTDFGQP 240
           LFTCQSVD RAGYMSSIQLNAI IDPRNPNLFVVAGSDEYARLYDIRK   +GSTDFGQ 
Sbjct: 181 LFTCQSVDNRAGYMSSIQLNAIVIDPRNPNLFVVAGSDEYARLYDIRKSGEDGSTDFGQL 240

Query: 241 ADCYCPPHLIGDEQVGITGLAFSELSELLVSYNDESIYLFNRDMGLGRSPYPWSP----- 300
           ADCYCPPHLIGDEQVGITGLAFSELSELLVSYNDESIYLFNRDMGLG +P P  P     
Sbjct: 241 ADCYCPPHLIGDEQVGITGLAFSELSELLVSYNDESIYLFNRDMGLGPNPNPVPPLSLSS 300

Query: 301 DTSDMGAEKVKDGSPQVYKGHRNCETVKGVNFFGPKCEYVISGSDCGRIFIWRKKGGGLI 360
           D S+MGAE V +G PQVYKGHRNCETVKGVNFFGPKCEYV+SGSDCGRIFIWRKKGG LI
Sbjct: 301 DASEMGAENVDNGIPQVYKGHRNCETVKGVNFFGPKCEYVVSGSDCGRIFIWRKKGGDLI 360

Query: 361 RVMEADEDVVNCIEPHPHMTMLASSGIESDVKLWTPKALERATLPEKIEQKLKPRGWMHR 420
           RVMEADEDVVNCIEPHPHMTMLASSGIESDVK+WTPKA ERATLPEKIEQK KPRGWMHR
Sbjct: 361 RVMEADEDVVNCIEPHPHMTMLASSGIESDVKIWTPKAHERATLPEKIEQKPKPRGWMHR 420

Query: 421 LALPEELMMQLFSLRRQNSSPEHRRENSAVGREILELIMTFNNNTESSSEDNGGDTAEDD 476
           LALPEELMMQLFSLRRQ SSPE  RE   V RE+LELI TFNN     S D   D+ EDD
Sbjct: 421 LALPEELMMQLFSLRRQTSSPESGREPPTVSRELLELIRTFNNGNSDDSSDYNDDSGEDD 480

[Close]

BLAST of Lag0001373 vs. ExPASy Swiss-Prot
Match: Q8N7N5 (DDB1- and CUL4-associated factor 8 OS=Mus musculus OX=10090 GN=Dcaf8 PE=1 SV=1)

HSP 1 Score: 293.1 bits (749), Expect = 5.6e-78
Identity = 181/466 (38.84%), Postives = 257/466 (55.15%), Query Frame = 0


Query: 19  KREVGELSSRDFAHRLGASEDLVLRLDLFRKLERHKGCVNTVSFSAAGDILVSGSDDRRV 78
           +RE+G  SS  F +    +   V R  L   LE H GCVNT+ F+  G  L SGSDD +V
Sbjct: 154 ERELG--SSARFVYEACGARVFVQRFRLQHGLEGHTGCVNTLHFNQRGTWLASGSDDLKV 213

Query: 79  ILWNWETGHAKLCFQSGHNNNVFQAKIMPYTDDNSIVTCAADGQVRHAQILDGGDVE-TA 138
           ++W+W      L F+SGH +NVFQAK +P + D+++  CA DGQVR A++      + T 
Sbjct: 214 VVWDWVRRQPVLDFESGHKSNVFQAKFLPNSGDSTLAMCARDGQVRVAELSATQCCKNTK 273

Query: 139 LLGSHQGRAHKLAIEPGSPYIFYTCGEDGLVQHFDLR-TRAAIELFTCQSVDKRAGYMSS 198
            +  H+G +HKLA+EP SP  F + GED +V   DLR  R A +L   +  +K+ G    
Sbjct: 274 RVAQHKGASHKLALEPDSPCTFLSAGEDAVVFTIDLRQDRPASKLVVTKEKEKKVG---- 333

Query: 199 IQLNAIAIDPRNPNLFVVAGSDEYARLYDIRKYKGEGSTDFGQPADCYCPPHLIGDE-QV 258
             L  I ++P N + F V G D+Y R+YD RK     +    +    +CP HL+  E + 
Sbjct: 334 --LYTIYVNPANTHQFAVGGRDQYVRIYDQRKIDENENNGVLKK---FCPHHLVNSESKA 393

Query: 259 GITGLAFS-ELSELLVSYNDESIYLFNRDMGLGRSPYPWSPDTSDMGAEKVKDGSPQVYK 318
            IT L +S + +ELL SYNDE IYLFN               +   GA+ +K      YK
Sbjct: 394 NITCLVYSHDGTELLASYNDEDIYLFN--------------SSHSDGAQYIKR-----YK 453

Query: 319 GHRNCETVKGVNFFGPKCEYVISGSDCGRIFIWRKKGGGLIRVMEADE-DVVNCIEPHPH 378
           GHRN  TVKGVNF+GPK E+V+SGSDCG IF+W K    +I+ ME D+  VVNC+EPHPH
Sbjct: 454 GHRNNATVKGVNFYGPKSEFVVSGSDCGHIFLWEKSSCQIIQFMEGDKGGVVNCLEPHPH 513

Query: 379 MTMLASSGIESDVKLWTPKA---LERATLPEKIEQKLKPR--GWMHRLALPEELMMQLFS 438
           + +LA+SG++ DVK+W P A    E   L E I++  + R    +H   L +  M+  F 
Sbjct: 514 LPVLATSGLDHDVKIWAPTAEASTELTGLKEVIKKNKRERDEDSLHHTDLFDSHML-WFL 573

Query: 439 LRRQNSSPEHRR-ENSAVGREILELIMTFNNNTESSSEDNGGDTAE 474
           +        HRR     VG    +   +  +++++S E+ G D  +
Sbjct: 574 MHHLRQRRHHRRWREPGVGATDADSDES-PSSSDTSDEEEGPDRVQ 587

[Close]

BLAST of Lag0001373 vs. ExPASy Swiss-Prot
Match: Q5TAQ9 (DDB1- and CUL4-associated factor 8 OS=Homo sapiens OX=9606 GN=DCAF8 PE=1 SV=1)

HSP 1 Score: 291.2 bits (744), Expect = 2.1e-77
Identity = 180/466 (38.63%), Postives = 258/466 (55.36%), Query Frame = 0


Query: 19  KREVGELSSRDFAHRLGASEDLVLRLDLFRKLERHKGCVNTVSFSAAGDILVSGSDDRRV 78
           +RE+G  SS  F +    +   V R  L   LE H GCVNT+ F+  G  L SGSDD +V
Sbjct: 160 ERELG--SSARFVYEACGARVFVQRFRLQHGLEGHTGCVNTLHFNQRGTWLASGSDDLKV 219

Query: 79  ILWNWETGHAKLCFQSGHNNNVFQAKIMPYTDDNSIVTCAADGQVRHAQILDGGDVE-TA 138
           ++W+W      L F+SGH +NVFQAK +P + D+++  CA DGQVR A++      + T 
Sbjct: 220 VVWDWVRRQPVLDFESGHKSNVFQAKFLPNSGDSTLAMCARDGQVRVAELSATQCCKNTK 279

Query: 139 LLGSHQGRAHKLAIEPGSPYIFYTCGEDGLVQHFDLR-TRAAIELFTCQSVDKRAGYMSS 198
            +  H+G +HKLA+EP SP  F + GED +V   DLR  R A +L   +  +K+ G    
Sbjct: 280 RVAQHKGASHKLALEPDSPCTFLSAGEDAVVFTIDLRQDRPASKLVVTKEKEKKVG---- 339

Query: 199 IQLNAIAIDPRNPNLFVVAGSDEYARLYDIRKYKGEGSTDFGQPADCYCPPHLIGDE-QV 258
             L  I ++P N + F V G D++ R+YD RK     +    +    +CP HL+  E + 
Sbjct: 340 --LYTIYVNPANTHQFAVGGRDQFVRIYDQRKIDENENNGVLKK---FCPHHLVNSESKA 399

Query: 259 GITGLAFS-ELSELLVSYNDESIYLFNRDMGLGRSPYPWSPDTSDMGAEKVKDGSPQVYK 318
            IT L +S + +ELL SYNDE IYLFN               +   GA+ VK      YK
Sbjct: 400 NITCLVYSHDGTELLASYNDEDIYLFN--------------SSHSDGAQYVKR-----YK 459

Query: 319 GHRNCETVKGVNFFGPKCEYVISGSDCGRIFIWRKKGGGLIRVMEADE-DVVNCIEPHPH 378
           GHRN  TVKGVNF+GPK E+V+SGSDCG IF+W K    +I+ ME D+  VVNC+EPHPH
Sbjct: 460 GHRNNATVKGVNFYGPKSEFVVSGSDCGHIFLWEKSSCQIIQFMEGDKGGVVNCLEPHPH 519

Query: 379 MTMLASSGIESDVKLWTPKA---LERATLPEKIEQKLKPR--GWMHRLALPEELMMQLFS 438
           + +LA+SG++ DVK+W P A    E   L + I++  + R    +H+  L +  M+  F 
Sbjct: 520 LPVLATSGLDHDVKIWAPTAEASTELTGLKDVIKKNKRERDEDSLHQTDLFDSHML-WFL 579

Query: 439 LRRQNSSPEHRR-ENSAVGREILELIMTFNNNTESSSEDNGGDTAE 474
           +        HRR     VG    +   +  +++++S E+ G D  +
Sbjct: 580 MHHLRQRRHHRRWREPGVGATDADSDES-PSSSDTSDEEEGPDRVQ 593

[Close]

BLAST of Lag0001373 vs. ExPASy Swiss-Prot
Match: Q5U2M6 (DDB1- and CUL4-associated factor 8 OS=Rattus norvegicus OX=10116 GN=Dcaf8 PE=1 SV=1)

HSP 1 Score: 290.4 bits (742), Expect = 3.6e-77
Identity = 179/466 (38.41%), Postives = 257/466 (55.15%), Query Frame = 0


Query: 19  KREVGELSSRDFAHRLGASEDLVLRLDLFRKLERHKGCVNTVSFSAAGDILVSGSDDRRV 78
           +RE+G  SS  F +    +   V R  L   LE H GCVNT+ F+  G  L SGSDD +V
Sbjct: 154 ERELG--SSARFVYEACGARVFVQRFRLQHGLEGHTGCVNTLHFNQRGTWLASGSDDLKV 213

Query: 79  ILWNWETGHAKLCFQSGHNNNVFQAKIMPYTDDNSIVTCAADGQVRHAQILDGGDVE-TA 138
           ++W+W      L F+SGH +NVFQAK +P + D+++  CA DGQVR A++      + T 
Sbjct: 214 VVWDWVRRQPVLDFESGHKSNVFQAKFLPNSGDSTLAMCARDGQVRVAELSATQCCKNTK 273

Query: 139 LLGSHQGRAHKLAIEPGSPYIFYTCGEDGLVQHFDLR-TRAAIELFTCQSVDKRAGYMSS 198
            +  H+G +HKLA+EP SP  F + GED +V   DLR  R A +L   +  +K+ G    
Sbjct: 274 RVAQHKGASHKLALEPDSPCTFLSAGEDAVVFTIDLRQDRPASKLVVTKEKEKKVG---- 333

Query: 199 IQLNAIAIDPRNPNLFVVAGSDEYARLYDIRKYKGEGSTDFGQPADCYCPPHLIGDE-QV 258
             L  I ++P N + F V G D++ R+YD RK     +    +    +CP HL+  E + 
Sbjct: 334 --LYTIYVNPANTHQFAVGGRDQFVRIYDQRKIDENENNGVLKK---FCPHHLVNSESKA 393

Query: 259 GITGLAFS-ELSELLVSYNDESIYLFNRDMGLGRSPYPWSPDTSDMGAEKVKDGSPQVYK 318
            IT L +S + +ELL SYNDE IYLFN               +   GA+ +K      YK
Sbjct: 394 NITCLVYSHDGTELLASYNDEDIYLFN--------------SSHSDGAQYIKR-----YK 453

Query: 319 GHRNCETVKGVNFFGPKCEYVISGSDCGRIFIWRKKGGGLIRVMEADE-DVVNCIEPHPH 378
           GHRN  TVKGVNF+GPK E+V+SGSDCG IF+W K    +I+ ME D+  VVNC+EPHPH
Sbjct: 454 GHRNNATVKGVNFYGPKSEFVVSGSDCGHIFLWEKSSCQIIQFMEGDKGGVVNCLEPHPH 513

Query: 379 MTMLASSGIESDVKLWTPKA---LERATLPEKIEQKLKPR--GWMHRLALPEELMMQLFS 438
           + +LA+SG++ DVK+W P A    E   L + I++  + R    +H   L +  M+  F 
Sbjct: 514 LPVLATSGLDHDVKIWAPTAEASTELTGLKDVIKKNKRERDEDSLHHTDLFDSHML-WFL 573

Query: 439 LRRQNSSPEHRR-ENSAVGREILELIMTFNNNTESSSEDNGGDTAE 474
           +        HRR     VG    +   +  +++++S E+ G D  +
Sbjct: 574 MHHLRQRRHHRRWREPGVGATDADSDES-PSSSDTSDEEEGPDRVQ 587

[Close]

BLAST of Lag0001373 vs. ExPASy Swiss-Prot
Match: Q6NRH1 (DDB1- and CUL4-associated factor 8 OS=Xenopus laevis OX=8355 GN=dcaf8 PE=2 SV=1)

HSP 1 Score: 290.0 bits (741), Expect = 4.7e-77
Identity = 175/474 (36.92%), Postives = 255/474 (53.80%), Query Frame = 0


Query: 12  KAVVNVWKREVGELSSRDFAHRLGASEDLVLRLDLFRKLERHKGCVNTVSFSAAGDILVS 71
           +A+  + +R++G  SS  F +    +   V R  L   L+ H GCVNT+ F+  G  L S
Sbjct: 156 RALSALRQRQMG--SSTRFIYEACGARGFVQRFHLLHGLDGHSGCVNTLHFNQRGTCLAS 215

Query: 72  GSDDRRVILWNWETGHAKLCFQSGHNNNVFQAKIMPYTDDNSIVTCAADGQVRHAQILDG 131
           GSDD +V++W+W      L F+SGH +NVFQAK +P + D+++  CA DGQVR A++   
Sbjct: 216 GSDDLKVVVWDWVRRKPVLEFESGHKSNVFQAKFLPNSGDSTLAMCARDGQVRVAELSAT 275

Query: 132 GDVE-TALLGSHQGRAHKLAIEPGSPYIFYTCGEDGLVQHFDLR-TRAAIELFTCQSVDK 191
              + T  +  H+G +HKLA+EP SP  F + GED +V   DLR  R A  L   +  + 
Sbjct: 276 HCCKNTKRVAQHKGASHKLALEPDSPCTFLSAGEDAVVFTIDLRQDRPASRLVVTKEKES 335

Query: 192 RAGYMSSIQLNAIAIDPRNPNLFVVAGSDEYARLYDIRKYKGEGSTDFGQPADCYCPPHL 251
           + G      L  I ++P N   F V G D++ R+YD RK     +    +    +CP HL
Sbjct: 336 KVG------LYTIYVNPANTYQFAVGGRDQFVRIYDQRKINENVNNGVLKK---FCPHHL 395

Query: 252 IGDE-QVGITGLAFS-ELSELLVSYNDESIYLFNRDMGLGRSPYPWSPDTSDMGAEKVKD 311
           +  E +  IT L +S + SELL SYNDE IYLFN               +   GAE +K 
Sbjct: 396 VTSEAKANITCLVYSHDGSELLASYNDEDIYLFN--------------SSHSDGAEYIKR 455

Query: 312 GSPQVYKGHRNCETVKGVNFFGPKCEYVISGSDCGRIFIWRKKGGGLIRVMEADE-DVVN 371
                YKGHRN  TVKGVNF+GP+ E+V+SGSDCG IF+W K    +++ M+ D+  VVN
Sbjct: 456 -----YKGHRNNATVKGVNFYGPRSEFVVSGSDCGHIFLWEKSSCQIVQFMDGDKGGVVN 515

Query: 372 CIEPHPHMTMLASSGIESDVKLWTPKALERAT---LPEKIEQKLKPR--GWMHRLALPEE 431
           C+EPHPH+ +LA+SG++ DVK+W P A E      L E I++  + R    +H   L + 
Sbjct: 516 CLEPHPHLPVLATSGLDYDVKIWLPTAKEPTELDGLKEVIKKNKRERDEDSLHHTDLFDN 575

Query: 432 LMMQLFSLRRQNSSPEHRRENSAVGREILELIMTFNNNTESSSEDNGGDTAEDD 476
            M+       +      RR ++ +G            + ES    +  D+++DD
Sbjct: 576 HMLWFLMHHLRQRGQRRRRRDAGLGA----------GDAESDDSPSSSDSSDDD 589

[Close]

BLAST of Lag0001373 vs. ExPASy Swiss-Prot
Match: Q5R448 (DDB1- and CUL4-associated factor 8 OS=Pongo abelii OX=9601 GN=DCAF8 PE=2 SV=1)

HSP 1 Score: 289.3 bits (739), Expect = 8.1e-77
Identity = 180/466 (38.63%), Postives = 258/466 (55.36%), Query Frame = 0


Query: 19  KREVGELSSRDFAHRLGASEDLVLRLDLFRKLERHKGCVNTVSFSAAGDILVSGSDDRRV 78
           +RE+G  SS  F +    +   V R  L   LE H GCVNT+ F+  G  L SGSDD +V
Sbjct: 160 ERELG--SSARFVYEACGARVFVQRFRLQHGLEGHTGCVNTLHFNQRGTWLASGSDDLKV 219

Query: 79  ILWNWETGHAKLCFQSGHNNNVFQAKIMPYTDDNSIVTCAADGQVRHAQILDGGDVE-TA 138
           ++W+W      L F+SGH +NVFQAK +P + D+++   A DGQVR A++      + T 
Sbjct: 220 VVWDWVRRQPVLDFESGHKSNVFQAKFLPNSGDSTLAMRARDGQVRVAELSATQCCKNTK 279

Query: 139 LLGSHQGRAHKLAIEPGSPYIFYTCGEDGLVQHFDLR-TRAAIELFTCQSVDKRAGYMSS 198
            +  H+G +HKLA+EP SP  F + GED +V   DLR  R A +L   +  +K+ G    
Sbjct: 280 RVAQHKGASHKLALEPDSPCTFLSAGEDAVVFTIDLRQDRPASKLVVTKEKEKKVG---- 339

Query: 199 IQLNAIAIDPRNPNLFVVAGSDEYARLYDIRKYKGEGSTDFGQPADCYCPPHLI-GDEQV 258
             L  I ++P N + F V G D++ R+YD RK     +    +    +CP HL+ G+ + 
Sbjct: 340 --LYTIYVNPANTHQFAVGGRDQFVRIYDQRKIDENENNGVLKK---FCPHHLVNGESKA 399

Query: 259 GITGLAFS-ELSELLVSYNDESIYLFNRDMGLGRSPYPWSPDTSDMGAEKVKDGSPQVYK 318
            IT L +S + +ELL SYNDE IYLFN               +   GA+ VK      YK
Sbjct: 400 NITCLVYSHDGTELLASYNDEDIYLFN--------------SSHSDGAQYVKR-----YK 459

Query: 319 GHRNCETVKGVNFFGPKCEYVISGSDCGRIFIWRKKGGGLIRVMEADE-DVVNCIEPHPH 378
           GHRN  TVKGVNF+GPK E+V+SGSDCG IF+W K    +I+ ME D+  VVNC+EPHPH
Sbjct: 460 GHRNNATVKGVNFYGPKSEFVVSGSDCGHIFLWEKSSCQIIQFMEGDKGGVVNCLEPHPH 519

Query: 379 MTMLASSGIESDVKLWTPKA---LERATLPEKIEQKLKPR--GWMHRLALPEELMMQLFS 438
           + +LA+SG++ DVK+W P A    E   L + I++  + R    +HR  L +  M+  F 
Sbjct: 520 LPVLATSGLDHDVKIWAPTAEASTELTGLKDVIKKNKRERDEDSLHRTDLFDSHML-WFL 579

Query: 439 LRRQNSSPEHRR-ENSAVGREILELIMTFNNNTESSSEDNGGDTAE 474
           +        HRR     VG    +   +  +++++S E+ G D  +
Sbjct: 580 MHHLRQRRHHRRWREPGVGATDADSDES-PSSSDTSDEEEGPDRVQ 593

[Close]

BLAST of Lag0001373 vs. ExPASy TrEMBL
Match: A0A6J1C6L2 (DDB1- and CUL4-associated factor 8 OS=Momordica charantia OX=3673 GN=LOC111007852 PE=4 SV=1)

HSP 1 Score: 876.7 bits (2264), Expect = 4.4e-251
Identity = 422/480 (87.92%), Postives = 448/480 (93.33%), Query Frame = 0


Query: 1   MNKRARISHSNKAVVNVWKREVGELSSRDFAHRLGASEDLVLRLDLFRKLERHKGCVNTV 60
           MNKRARI  SNKA+VNVWKREVGELS+R+FAHRLGAS+DLVLRLD++RKLERHKGCVNTV
Sbjct: 1   MNKRARIDDSNKALVNVWKREVGELSTRNFAHRLGASQDLVLRLDIYRKLERHKGCVNTV 60

Query: 61  SFSAAGDILVSGSDDRRVILWNWETGHAKLCFQSGHNNNVFQAKIMPYTDDNSIVTCAAD 120
           SF+AAGD+LVSGSDDRRVILWNWETG  KL FQSGHNNNVFQAKIMPYTDD  IVTCAAD
Sbjct: 61  SFNAAGDVLVSGSDDRRVILWNWETGQVKLSFQSGHNNNVFQAKIMPYTDDRCIVTCAAD 120

Query: 121 GQVRHAQILDGGDVETALLGSHQGRAHKLAIEPGSPYIFYTCGEDGLVQHFDLRTRAAIE 180
           GQVRHAQIL+ G VET+LLGSHQGRAHKLAIEPGSPYIFYTCGEDGLVQHFDLRT AA E
Sbjct: 121 GQVRHAQILNSGRVETSLLGSHQGRAHKLAIEPGSPYIFYTCGEDGLVQHFDLRTGAASE 180

Query: 181 LFTCQSVDKRAGYMSSIQLNAIAIDPRNPNLFVVAGSDEYARLYDIRKYKGEGSTDFGQP 240
           LFTCQS+D RAGYMSSIQLNAI IDPRNPNLFVVAGSDEYARLYDIRKYKGEGSTDFGQP
Sbjct: 181 LFTCQSIDNRAGYMSSIQLNAITIDPRNPNLFVVAGSDEYARLYDIRKYKGEGSTDFGQP 240

Query: 241 ADCYCPPHLIGDEQVGITGLAFSELSELLVSYNDESIYLFNRDMGLGRSPYPWSP----- 300
            D YCPPHLIGDEQVGITGLA+SELSELLVSYNDESIYLFNRDMGLG +P P SP     
Sbjct: 241 TDYYCPPHLIGDEQVGITGLAYSELSELLVSYNDESIYLFNRDMGLGPNPNPVSPVSMSS 300

Query: 301 DTSDMGAEKVKDGSPQVYKGHRNCETVKGVNFFGPKCEYVISGSDCGRIFIWRKKGGGLI 360
           D+S+MGAE V+ GSPQVYKGHRNCETVKGVNFFGPKCEYV+SGSDCGRIFIW+KKGG LI
Sbjct: 301 DSSEMGAENVEHGSPQVYKGHRNCETVKGVNFFGPKCEYVVSGSDCGRIFIWKKKGGELI 360

Query: 361 RVMEADEDVVNCIEPHPHMTMLASSGIESDVKLWTPKALERATLPEKIEQKLKPRGWMHR 420
           RVMEADEDVVNCIEPHPH+TMLASSGIESD+K+WTPKALERATLPEKIEQK KPRGWMHR
Sbjct: 361 RVMEADEDVVNCIEPHPHVTMLASSGIESDIKIWTPKALERATLPEKIEQKPKPRGWMHR 420

Query: 421 LALPEELMMQLFSLRRQNSSPEHRRENSAVGREILELIMTFNNNTESSSEDNGGDTAEDD 476
           LALPEELMMQLFSLRR+NSSPE RRENSAV REILELI+TFN N++ SS+DNG DTAEDD
Sbjct: 421 LALPEELMMQLFSLRRRNSSPERRRENSAVSREILELILTFNANSDESSDDNGDDTAEDD 480

[Close]

BLAST of Lag0001373 vs. ExPASy TrEMBL
Match: A0A1S3BIJ4 (DDB1- and CUL4-associated factor 8 OS=Cucumis melo OX=3656 GN=LOC103490261 PE=4 SV=1)

HSP 1 Score: 845.5 bits (2183), Expect = 1.1e-241
Identity = 414/480 (86.25%), Postives = 430/480 (89.58%), Query Frame = 0


Query: 1   MNKRARISHSNKAVVNVWKREVGELSSRDFAHRLGASEDLVLRLDLFRKLERHKGCVNTV 60
           MNKRARI  SNKAVVNVWKREVGELS+R+FAHRLGA EDLVLRLDL++KLERHKGCVNTV
Sbjct: 1   MNKRARIDDSNKAVVNVWKREVGELSTRNFAHRLGALEDLVLRLDLYKKLERHKGCVNTV 60

Query: 61  SFSAAGDILVSGSDDRRVILWNWETGHAKLCFQSGHNNNVFQAKIMPYTDDNSIVTCAAD 120
           SF+AAGDILVSGSDDRRVILWNWETG  KL FQSGHNNNVFQAKIMPYTDD SIVTCAAD
Sbjct: 61  SFNAAGDILVSGSDDRRVILWNWETGRVKLSFQSGHNNNVFQAKIMPYTDDRSIVTCAAD 120

Query: 121 GQVRHAQILDGGDVETALLGSHQGRAHKLAIEPGSPYIFYTCGEDGLVQHFDLRTRAAIE 180
           GQVRHAQIL+ G VET+LLGSH GRAHKLAIEPGSPY+FYTCGEDGLVQ FDLRT AA+E
Sbjct: 121 GQVRHAQILNSGHVETSLLGSHLGRAHKLAIEPGSPYMFYTCGEDGLVQRFDLRTGAAVE 180

Query: 181 LFTCQSVDKRAGYMSSIQLNAIAIDPRNPNLFVVAGSDEYARLYDIRKYKGEGSTDFGQP 240
           LFTCQSVD RAGYMSSIQLNAI IDPRNPNLFVVAGSDEYARLYDIRK   +GSTDFGQ 
Sbjct: 181 LFTCQSVDNRAGYMSSIQLNAIVIDPRNPNLFVVAGSDEYARLYDIRKSGEDGSTDFGQL 240

Query: 241 ADCYCPPHLIGDEQVGITGLAFSELSELLVSYNDESIYLFNRDMGLGRSPYPWSP----- 300
           ADCYCPPHLIGDEQVGITGLAFSELSELLVSYNDESIYLFNRDMGLG +P P  P     
Sbjct: 241 ADCYCPPHLIGDEQVGITGLAFSELSELLVSYNDESIYLFNRDMGLGPNPNPAPPLSLSS 300

Query: 301 DTSDMGAEKVKDGSPQVYKGHRNCETVKGVNFFGPKCEYVISGSDCGRIFIWRKKGGGLI 360
           D S+MGAEKV +G PQVYKGHRNCETVKGVNFFGPKCEYV+SGSDCGRIFIWRKKGG LI
Sbjct: 301 DASEMGAEKVDNGIPQVYKGHRNCETVKGVNFFGPKCEYVVSGSDCGRIFIWRKKGGELI 360

Query: 361 RVMEADEDVVNCIEPHPHMTMLASSGIESDVKLWTPKALERATLPEKIEQKLKPRGWMHR 420
           RVMEADEDVVNCIEPHPHMTMLASSGIESDVK+WTPKA ERATLPEKIEQK KPRGWMHR
Sbjct: 361 RVMEADEDVVNCIEPHPHMTMLASSGIESDVKIWTPKAHERATLPEKIEQKPKPRGWMHR 420

Query: 421 LALPEELMMQLFSLRRQNSSPEHRRENSAVGREILELIMTFNNNTESSSEDNGGDTAEDD 476
           LALPEELMMQLFSLRRQ SSPE  RE   V RE+LELIMTFNN     S D   DT EDD
Sbjct: 421 LALPEELMMQLFSLRRQTSSPERGREPPTVSRELLELIMTFNNGNSDDSSDYNDDTGEDD 480

[Close]

BLAST of Lag0001373 vs. ExPASy TrEMBL
Match: A0A5D3DIH7 (DDB1-and CUL4-associated factor 8 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold142G004590 PE=4 SV=1)

HSP 1 Score: 840.1 bits (2169), Expect = 4.5e-240
Identity = 412/480 (85.83%), Postives = 428/480 (89.17%), Query Frame = 0


Query: 1   MNKRARISHSNKAVVNVWKREVGELSSRDFAHRLGASEDLVLRLDLFRKLERHKGCVNTV 60
           MNKRARI  SNKAVVNVWKREVGELS+R+FAHRLGA EDLVLRLDL++KLERHKGCVNTV
Sbjct: 1   MNKRARIDDSNKAVVNVWKREVGELSTRNFAHRLGALEDLVLRLDLYKKLERHKGCVNTV 60

Query: 61  SFSAAGDILVSGSDDRRVILWNWETGHAKLCFQSGHNNNVFQAKIMPYTDDNSIVTCAAD 120
           SF+AAGDILVSGSDDRRVILWNWETG  KL FQSGHNNNVFQAKIMPYTDD SIVTCAAD
Sbjct: 61  SFNAAGDILVSGSDDRRVILWNWETGRVKLSFQSGHNNNVFQAKIMPYTDDRSIVTCAAD 120

Query: 121 GQVRHAQILDGGDVETALLGSHQGRAHKLAIEPGSPYIFYTCGEDGLVQHFDLRTRAAIE 180
           GQVRHAQIL+ G VET+LLGSH GRAHKLAIEPGSPY+FYTCGEDGLVQ FDLRT AA+E
Sbjct: 121 GQVRHAQILNSGHVETSLLGSHLGRAHKLAIEPGSPYMFYTCGEDGLVQRFDLRTGAAVE 180

Query: 181 LFTCQSVDKRAGYMSSIQLNAIAIDPRNPNLFVVAGSDEYARLYDIRKYKGEGSTDFGQP 240
           LFTCQSVD RAGYMSSIQLNAI IDPRNPNLFVVAGSDEYARLYDIRK   +GSTDFGQ 
Sbjct: 181 LFTCQSVDNRAGYMSSIQLNAIVIDPRNPNLFVVAGSDEYARLYDIRKSGEDGSTDFGQL 240

Query: 241 ADCYCPPHLIGDEQVGITGLAFSELSELLVSYNDESIYLFNRDMGLGRSPYPWSP----- 300
            D YCPPHLIGDEQVGITGLAFSELSELLVSYNDESIYLFNRDMGLG +P P  P     
Sbjct: 241 VDSYCPPHLIGDEQVGITGLAFSELSELLVSYNDESIYLFNRDMGLGPNPNPAPPLSLSS 300

Query: 301 DTSDMGAEKVKDGSPQVYKGHRNCETVKGVNFFGPKCEYVISGSDCGRIFIWRKKGGGLI 360
           D S+MGAEKV +G PQVYKGHRNCETVKGVNFFGPKCEYV+SGSDCGRIFIWRKKGG LI
Sbjct: 301 DASEMGAEKVDNGIPQVYKGHRNCETVKGVNFFGPKCEYVVSGSDCGRIFIWRKKGGELI 360

Query: 361 RVMEADEDVVNCIEPHPHMTMLASSGIESDVKLWTPKALERATLPEKIEQKLKPRGWMHR 420
           RVMEADEDVVNCIEPHPHMTMLASSGIESDVK+WTPKA ERATLPEKIEQK KPRGWMHR
Sbjct: 361 RVMEADEDVVNCIEPHPHMTMLASSGIESDVKIWTPKAHERATLPEKIEQKPKPRGWMHR 420

Query: 421 LALPEELMMQLFSLRRQNSSPEHRRENSAVGREILELIMTFNNNTESSSEDNGGDTAEDD 476
           LALPEELMMQLFSLRRQ SSPE  RE   V RE+LELIMTFNN     S D   DT EDD
Sbjct: 421 LALPEELMMQLFSLRRQTSSPERGREPPTVSRELLELIMTFNNGNSDDSSDYNDDTGEDD 480

[Close]

BLAST of Lag0001373 vs. ExPASy TrEMBL
Match: A0A0A0K4R4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G016620 PE=4 SV=1)

HSP 1 Score: 836.3 bits (2159), Expect = 6.5e-239
Identity = 409/480 (85.21%), Postives = 427/480 (88.96%), Query Frame = 0


Query: 1   MNKRARISHSNKAVVNVWKREVGELSSRDFAHRLGASEDLVLRLDLFRKLERHKGCVNTV 60
           MNKRARI  SNKAVVNVWKREVGELS+R+FAHRLGA EDLVLRLDL++KLERHKGCVNTV
Sbjct: 1   MNKRARIDDSNKAVVNVWKREVGELSTRNFAHRLGALEDLVLRLDLYKKLERHKGCVNTV 60

Query: 61  SFSAAGDILVSGSDDRRVILWNWETGHAKLCFQSGHNNNVFQAKIMPYTDDNSIVTCAAD 120
           SF+AAGDILVSGSDDRRVILWNWETG  KL FQSGHNNNVFQAKIMPYTDD SIVTCAAD
Sbjct: 61  SFNAAGDILVSGSDDRRVILWNWETGRVKLSFQSGHNNNVFQAKIMPYTDDQSIVTCAAD 120

Query: 121 GQVRHAQILDGGDVETALLGSHQGRAHKLAIEPGSPYIFYTCGEDGLVQHFDLRTRAAIE 180
           GQVRHAQIL+ G VET+LLG+H GRAHKLAIEPGSPY+FYTCGEDGLVQ FDLRT  A+E
Sbjct: 121 GQVRHAQILNSGHVETSLLGNHLGRAHKLAIEPGSPYMFYTCGEDGLVQRFDLRTGDAVE 180

Query: 181 LFTCQSVDKRAGYMSSIQLNAIAIDPRNPNLFVVAGSDEYARLYDIRKYKGEGSTDFGQP 240
           LFTCQSVD RAGYMSSIQLNAI IDPRNPNLFVVAGSDEYARLYDIRK   +GSTDFGQ 
Sbjct: 181 LFTCQSVDNRAGYMSSIQLNAIVIDPRNPNLFVVAGSDEYARLYDIRKSGEDGSTDFGQL 240

Query: 241 ADCYCPPHLIGDEQVGITGLAFSELSELLVSYNDESIYLFNRDMGLGRSPYPWSP----- 300
           ADCYCPPHLIGDEQVGITGLAFSELSELLVSYNDESIYLFNRDMGLG +P P  P     
Sbjct: 241 ADCYCPPHLIGDEQVGITGLAFSELSELLVSYNDESIYLFNRDMGLGPNPNPVPPLSLSS 300

Query: 301 DTSDMGAEKVKDGSPQVYKGHRNCETVKGVNFFGPKCEYVISGSDCGRIFIWRKKGGGLI 360
           D S+MGAE V +G PQVYKGHRNCETVKGVNFFGPKCEYV+SGSDCGRIFIWRKKGG LI
Sbjct: 301 DASEMGAENVDNGIPQVYKGHRNCETVKGVNFFGPKCEYVVSGSDCGRIFIWRKKGGDLI 360

Query: 361 RVMEADEDVVNCIEPHPHMTMLASSGIESDVKLWTPKALERATLPEKIEQKLKPRGWMHR 420
           RVMEADEDVVNCIEPHPHMTMLASSGIESDVK+WTPKA ERATLPEKIEQK KPRGWMHR
Sbjct: 361 RVMEADEDVVNCIEPHPHMTMLASSGIESDVKIWTPKAHERATLPEKIEQKPKPRGWMHR 420

Query: 421 LALPEELMMQLFSLRRQNSSPEHRRENSAVGREILELIMTFNNNTESSSEDNGGDTAEDD 476
           LALPEELMMQLFSLRRQ SSPE  RE   V RE+LELI TFNN     S D   D+ EDD
Sbjct: 421 LALPEELMMQLFSLRRQTSSPESGREPPTVSRELLELIRTFNNGNSDDSSDYNDDSGEDD 480

[Close]

BLAST of Lag0001373 vs. ExPASy TrEMBL
Match: A0A6J1HVC3 (DDB1- and CUL4-associated factor 8-like OS=Cucurbita maxima OX=3661 GN=LOC111468181 PE=4 SV=1)

HSP 1 Score: 798.9 bits (2062), Expect = 1.2e-227
Identity = 395/483 (81.78%), Postives = 427/483 (88.41%), Query Frame = 0


Query: 1   MNKRARISHSNKAVVNVWKREVGELSSRDFAHRLGASEDLVLRLDLFRKLERHKGCVNTV 60
           MNKRAR   S+KAVVN  KREVGE+S+R+FA+RLGA EDLVLRL L RKLERHKGCVNTV
Sbjct: 1   MNKRARFDDSSKAVVNFLKREVGEISTRNFANRLGALEDLVLRLGLLRKLERHKGCVNTV 60

Query: 61  SFSAAGDILVSGSDDRRVILWNWETGHAKLCFQSGHNNNVFQAKIMPYTDDNSIVTCAAD 120
           SF+AAGD+LVSGSDDRRVILWNWE G  ++ FQSGHNNNVFQAK MPYTDD SIVTCAAD
Sbjct: 61  SFNAAGDVLVSGSDDRRVILWNWEAGQVEISFQSGHNNNVFQAKFMPYTDDRSIVTCAAD 120

Query: 121 GQVRHAQILDGGDVETALLGSHQGRAHKLAIEPGSPYIFYTCGEDGLVQHFDLRTRAAIE 180
           GQVRHAQ+L+GG VET+LLGSHQGRAHKLAIEPGSPYIFYTCGEDGLVQ FDLRT AA+E
Sbjct: 121 GQVRHAQVLNGGRVETSLLGSHQGRAHKLAIEPGSPYIFYTCGEDGLVQRFDLRTGAAVE 180

Query: 181 LFTCQSVDKRAGYMSSIQLNAIAIDPRNPNLFVVAGSDEYARLYDIRKYKGEG--STDFG 240
           LFTCQSV  RAGYMS++QLNAIA+DPRNPNL VVAGSDEYARLYDIRK  GEG  STD G
Sbjct: 181 LFTCQSVGNRAGYMSTVQLNAIAMDPRNPNLVVVAGSDEYARLYDIRKCGGEGSTSTDSG 240

Query: 241 QPADCYCPPHLIGDEQVGITGLAFSELSELLVSYNDESIYLFNRDMGLGRSPYPWSP--- 300
           +PADCYCPPHLI DEQVGITGLAFSELSELLVSYNDE IYLFNRDMGLG +P   SP   
Sbjct: 241 KPADCYCPPHLIADEQVGITGLAFSELSELLVSYNDEYIYLFNRDMGLGPNPNAVSPVSL 300

Query: 301 --DTSDMGAEKVKDGSPQVYKGHRNCETVKGVNFFGPKCEYVISGSDCGRIFIWRKKGGG 360
             DTS+MGAEKV + +PQVYKGHRNCETVKGVNFFGPKCEYVISGSDCGRIFIWRKKGG 
Sbjct: 301 SSDTSEMGAEKVVNCTPQVYKGHRNCETVKGVNFFGPKCEYVISGSDCGRIFIWRKKGGE 360

Query: 361 LIRVMEADEDVVNCIEPHPHMTMLASSGIESDVKLWTPKALERATLPEKIEQKLKPRGWM 420
           LIRVM+ADEDVVNCIEPHPHMTMLASSGIESDVK+WTP +  RATLPEKIEQ  KPRGWM
Sbjct: 361 LIRVMKADEDVVNCIEPHPHMTMLASSGIESDVKIWTPNSRHRATLPEKIEQNPKPRGWM 420

Query: 421 HRLALPEELMMQLFSL-RRQNSSPEHRRENSAVGREILELIMTF-NNNTESSSEDNGGDT 475
           HRLALPEE+MMQLFSL RR+NS+P   RENS   R+ILELIMTF N+N++ SS+ N GD 
Sbjct: 421 HRLALPEEVMMQLFSLRRRRNSNPVRGRENSTPNRDILELIMTFSNSNSDGSSDYNAGDA 480

[Close]

BLAST of Lag0001373 vs. TAIR 10
Match: AT4G35140.1 (Transducin/WD40 repeat-like superfamily protein )

HSP 1 Score: 650.6 bits (1677), Expect = 9.8e-187
Identity = 320/493 (64.91%), Postives = 390/493 (79.11%), Query Frame = 0


Query: 1   MNKRARISHSN-----KAVVNVWKREVGELSSRDFAHRLGASEDLVLRLDLFRKLERHKG 60
           M+++AR   SN       VV+ W+REVG +SSR+F++R  ASE+LVLRL++++KLE+HKG
Sbjct: 1   MSEKARRGRSNFNGLHHPVVDFWRREVGGISSRNFSNRFSASENLVLRLEIYKKLEKHKG 60

Query: 61  CVNTVSFSAAGDILVSGSDDRRVILWNWETGHAKLCFQSGHNNNVFQAKIMPYTDDNSIV 120
           CVNTVSF+A GD+L+SGSDDRRV+LW+W+ G+ KL F SGH NNVFQAK MP++DD +IV
Sbjct: 61  CVNTVSFNAEGDVLISGSDDRRVVLWDWQLGNVKLSFHSGHANNVFQAKFMPFSDDRTIV 120

Query: 121 TCAADGQVRHAQILDGGDVETALLGSHQGRAHKLAIEPGSPYIFYTCGEDGLVQHFDLRT 180
           TCAADG VR A IL+G  VET+ LG HQGRAHKL IEPG+P+IFYTCGEDGLVQ FDLRT
Sbjct: 121 TCAADGMVRRASILEGDKVETSFLGLHQGRAHKLCIEPGNPHIFYTCGEDGLVQRFDLRT 180

Query: 181 RAAIELFTCQSVDKRAGYMSSIQLNAIAIDPRNPNLFVVAGSDEYARLYDIRKYKGEGST 240
            A  ELFTC+SVD R   M +IQLNAIAIDPRN NLF V G +EYARLYDIR+++GEG  
Sbjct: 181 EAPTELFTCRSVDPRRRNMDAIQLNAIAIDPRNSNLFAVGGMEEYARLYDIRRFQGEGLN 240

Query: 241 DFGQPADCYCPPHLIGDEQVGITGLAFSELSELLVSYNDESIYLFNRDMGLGRSPYPWSP 300
            F + AD +CPPHLIG+E VGITGLAFSE SELLVSYNDE IYLF   MGLG +P P SP
Sbjct: 241 GFTRAADHFCPPHLIGNEDVGITGLAFSEQSELLVSYNDEFIYLFTPGMGLGSNPIPSSP 300

Query: 301 DTSDMGAEKVKDGSPQ---------VYKGHRNCETVKGVNFFGPKCEYVISGSDCGRIFI 360
            +    ++     SP+         VYKGH+NCETVKGVNFFGP+ EYV+SGSDCGRIFI
Sbjct: 301 ISKSPVSKSESSSSPKDENEHSVSLVYKGHKNCETVKGVNFFGPRSEYVVSGSDCGRIFI 360

Query: 361 WRKKGGGLIRVMEADEDVVNCIEPHPHMTMLASSGIESDVKLWTPKALERATLPEKIE-Q 420
           WRKKGG LIRVMEAD  VVNCIEPHPH+ +LASSGIESD+K+WT KA ERATLPE IE +
Sbjct: 361 WRKKGGELIRVMEADRHVVNCIEPHPHIPVLASSGIESDIKVWTSKAAERATLPENIELR 420

Query: 421 KLKPRGWMHRLALPEELMMQLFSLR-RQNSSPEHRRE-NSAVGREILELIMTFNNNT--E 475
           K  PRGWM+R++ P EL+ QLFSL+ R +SSPE   E +SA GRE+L+LI+TFN+ +  E
Sbjct: 421 KRTPRGWMYRVSSPHELLAQLFSLQNRSSSSPEREGESSSATGRELLDLILTFNDQSDDE 480

[Close]

BLAST of Lag0001373 vs. TAIR 10
Match: AT4G38480.1 (Transducin/WD40 repeat-like superfamily protein )

HSP 1 Score: 531.6 bits (1368), Expect = 6.6e-151
Identity = 271/478 (56.69%), Postives = 329/478 (68.83%), Query Frame = 0


Query: 3   KRARISHSNKAVVNVWKREVGELSSRDFAHRLGASEDLVLRLDLFRKLERHKGCVNTVSF 62
           KR R  HS   VVNVW+RE+G L +R F++R  ASEDL+ RL L +KL++HKGCVNTVSF
Sbjct: 7   KRPRTDHS---VVNVWERELGLLPNRSFSNRFSASEDLLRRLGLDKKLDKHKGCVNTVSF 66

Query: 63  SAAGDILVSGSDDRRVILWNWETGHAKLCFQSGHNNNVFQAKIMPYTDDNSIVTCAADGQ 122
           +A GDIL+SGSDDR+VILW+W+T   KL F SGH NN+FQAK MP++DD +IVT AAD Q
Sbjct: 67  NADGDILLSGSDDRQVILWDWQTASVKLSFDSGHFNNIFQAKFMPFSDDRTIVTSAADKQ 126

Query: 123 VRHAQILDGGDVETALLGSHQGRAHKLAIEPGSPYIFYTCGEDGLVQHFDLRTRAAIELF 182
           VR+++IL+ G VET+LLG HQG  HKLA+EPGSP+ FYTCGEDG V+HFDLRTR A  LF
Sbjct: 127 VRYSKILESGQVETSLLGKHQGPVHKLAVEPGSPFSFYTCGEDGAVKHFDLRTRVATNLF 186

Query: 183 TCQSVDKRAGYMSSIQLNAIAIDPRNPNLFVVAGSDEYARLYDIRKYKGEGSTDFGQPAD 242
           TC    K A +   + L+AIA+DPRNP L  VAG DEYAR+YDIR Y+ EG  +F QP D
Sbjct: 187 TC----KEAKFNLVVYLHAIAVDPRNPGLLAVAGMDEYARVYDIRSYRSEGWYNFTQPID 246

Query: 243 CYCPPHLIGDEQVGITGLAFSELSELLVSYNDESIYLFNRDMGLGRSPYPWSPDTSDMGA 302
            +CP HLIGD+ VGITGLAFS+ SELL SY+DE IYLF  DMGLG +PYP S  T +   
Sbjct: 247 HFCPGHLIGDDHVGITGLAFSDQSELLASYSDEFIYLFTPDMGLGPTPYPSSTKTEER-- 306

Query: 303 EKVKDGSPQVYKGHRNCETVKGVNFFGPKCEYVISGSDCGRIFIWRKKGGGLIRVMEADE 362
                 +PQVYK H N ETVKGVNFFGPKCEYV+SGSDCGRIFIWRKK G L+R MEAD 
Sbjct: 307 -----MTPQVYKEHTNRETVKGVNFFGPKCEYVVSGSDCGRIFIWRKKDGELLRAMEADR 366

Query: 363 DVVNCIEPHPHMTMLASSGIESDVKLWTPKALERATLPEKIEQKL---KPRGWMHRLALP 422
            VVNCIE HPHM ++ SSGI++D+K+WTP   E+   P   +Q      PR W     + 
Sbjct: 367 HVVNCIESHPHMPLMCSSGIDTDIKIWTPGGTEKPLSPGNAKQASGFGNPR-WFDGYYVD 426

Query: 423 EELMMQLFSLRRQNSSPE--HRRENSAVGREILELIMTFNNNTESSSEDNGGDTAEDD 476
           ++      S    +   +     EN  V  EI          T+    D+G D  +DD
Sbjct: 427 DDDDSDDESSEESSDDDDSSEEEENGEVDVEI----------TKDDDNDHGDDDDDDD 459

[Close]

BLAST of Lag0001373 vs. TAIR 10
Match: AT3G45620.1 (Transducin/WD40 repeat-like superfamily protein )

HSP 1 Score: 427.2 bits (1097), Expect = 1.8e-119
Identity = 222/458 (48.47%), Postives = 294/458 (64.19%), Query Frame = 0


Query: 4   RARISHSNKAVVNVWKREVGELSSRDFAHRLGASEDLVLRLDLFRKLERHKGCVNTVSFS 63
           R R +        ++ RE+G       + R+ ASE  V +LDL+ KL  H+GCVN V F+
Sbjct: 5   RRRNTEIGDGFTEIFNREIGFSHPITISRRISASEGRVKKLDLYGKLNGHEGCVNAVEFN 64

Query: 64  AAGDILVSGSDDRRVILWNWETGHAKLCFQSGHNNNVFQAKIMPYTDDNSIVTCAADGQV 123
           + GD+LVSGSDDR+++LWNW +G  KL + SGH  NVFQ K +P+TDD +I+T  ADGQV
Sbjct: 65  STGDVLVSGSDDRQIMLWNWLSGSRKLSYPSGHCENVFQTKFIPFTDDRTIITSGADGQV 124

Query: 124 RHAQILDGGDVETALLGSHQGRAHKLAIEPGSPYIFYTCGEDGLVQHFDLRTRAA-IELF 183
           R  QIL+ G VET  LG H GR +KLA+ PG P +FY+CGEDG VQHFD+R+ +A + L+
Sbjct: 125 RLGQILENGKVETKRLGRHHGRVYKLAVLPGDPNVFYSCGEDGFVQHFDIRSNSATMVLY 184

Query: 184 TCQSVD--KRAGYMSSIQLNAIAIDPRNPNLFVVAGSDEYARLYDIRKYKGEGSTDF--- 243
           +       +R    S I+LN+IAIDPRN     V GSDEYAR+YD R+ +          
Sbjct: 185 SSPFTQGCRRHHSSSRIRLNSIAIDPRNSYYLAVGGSDEYARVYDTRRVQLAPVCRHVLP 244

Query: 244 GQPADCYCPPHLIGDEQVGITGLAFSELSELLVSYNDESIYLFNRDMGLGRSPYPWSPDT 303
             P + +CP HL     V ITGLA+S+  ELLVSYNDE IYLF ++MG G SP   SP  
Sbjct: 245 DAPVNTFCPRHLRETNSVHITGLAYSKAGELLVSYNDELIYLFEKNMGYGSSPVSVSP-- 304

Query: 304 SDMGAEKVKD-GSPQVYKGHRNCETVKGVNFFGPKCEYVISGSDCGRIFIWRKKGGGLIR 363
                EK+++   PQVY GHRN +TVKGVNFFGP  EYV SGSDCG IFIW+KKGG L+R
Sbjct: 305 -----EKLQEMEEPQVYIGHRNAQTVKGVNFFGPNDEYVTSGSDCGHIFIWKKKGGKLVR 364

Query: 364 VMEADEDVVNCIEPHPHMTMLASSGIESDVKLWTPKALERATLPEKIEQKLK----PRGW 423
            M  D  VVN +E HPH+ +LAS GIE  VKLWTP + +  +LPEKI++ ++     R  
Sbjct: 365 AMVGDRRVVNQLESHPHIPLLASCGIEKSVKLWTPMSNDVLSLPEKIDKVMELNRVGRED 424

Query: 424 MHRLALPEELMMQLFSLRRQNSS--PEHRRENSAVGRE 449
             R+ L  +++M +  L+R+ +S   E R  ++ +G +
Sbjct: 425 QSRVTLTPDVIMHVLRLQRRQTSAFTERRYVSTDIGSD 455

[Close]

BLAST of Lag0001373 vs. TAIR 10
Match: AT3G45620.2 (Transducin/WD40 repeat-like superfamily protein )

HSP 1 Score: 414.1 bits (1063), Expect = 1.5e-115
Identity = 213/421 (50.59%), Postives = 279/421 (66.27%), Query Frame = 0


Query: 41  VLRLDLFRKLERHKGCVNTVSFSAAGDILVSGSDDRRVILWNWETGHAKLCFQSGHNNNV 100
           V +LDL+ KL  H+GCVN V F++ GD+LVSGSDDR+++LWNW +G  KL + SGH  NV
Sbjct: 76  VKKLDLYGKLNGHEGCVNAVEFNSTGDVLVSGSDDRQIMLWNWLSGSRKLSYPSGHCENV 135

Query: 101 FQAKIMPYTDDNSIVTCAADGQVRHAQILDGGDVETALLGSHQGRAHKLAIEPGSPYIFY 160
           FQ K +P+TDD +I+T  ADGQVR  QIL+ G VET  LG H GR +KLA+ PG P +FY
Sbjct: 136 FQTKFIPFTDDRTIITSGADGQVRLGQILENGKVETKRLGRHHGRVYKLAVLPGDPNVFY 195

Query: 161 TCGEDGLVQHFDLRTRAA-IELFTCQSVD--KRAGYMSSIQLNAIAIDPRNPNLFVVAGS 220
           +CGEDG VQHFD+R+ +A + L++       +R    S I+LN+IAIDPRN     V GS
Sbjct: 196 SCGEDGFVQHFDIRSNSATMVLYSSPFTQGCRRHHSSSRIRLNSIAIDPRNSYYLAVGGS 255

Query: 221 DEYARLYDIRKYKGEGSTDF---GQPADCYCPPHLIGDEQVGITGLAFSELSELLVSYND 280
           DEYAR+YD R+ +            P + +CP HL     V ITGLA+S+  ELLVSYND
Sbjct: 256 DEYARVYDTRRVQLAPVCRHVLPDAPVNTFCPRHLRETNSVHITGLAYSKAGELLVSYND 315

Query: 281 ESIYLFNRDMGLGRSPYPWSPDTSDMGAEKVKD-GSPQVYKGHRNCETVKGVNFFGPKCE 340
           E IYLF ++MG G SP   SP       EK+++   PQVY GHRN +TVKGVNFFGP  E
Sbjct: 316 ELIYLFEKNMGYGSSPVSVSP-------EKLQEMEEPQVYIGHRNAQTVKGVNFFGPNDE 375

Query: 341 YVISGSDCGRIFIWRKKGGGLIRVMEADEDVVNCIEPHPHMTMLASSGIESDVKLWTPKA 400
           YV SGSDCG IFIW+KKGG L+R M  D  VVN +E HPH+ +LAS GIE  VKLWTP +
Sbjct: 376 YVTSGSDCGHIFIWKKKGGKLVRAMVGDRRVVNQLESHPHIPLLASCGIEKSVKLWTPMS 435

Query: 401 LERATLPEKIEQKLK----PRGWMHRLALPEELMMQLFSLRRQNSS--PEHRRENSAVGR 449
            +  +LPEKI++ ++     R    R+ L  +++M +  L+R+ +S   E R  ++ +G 
Sbjct: 436 NDVLSLPEKIDKVMELNRVGREDQSRVTLTPDVIMHVLRLQRRQTSAFTERRYVSTDIGS 489

[Close]

BLAST of Lag0001373 vs. TAIR 10
Match: AT5G10940.2 (transducin family protein / WD-40 repeat family protein )

HSP 1 Score: 152.1 bits (383), Expect = 1.1e-36
Identity = 95/295 (32.20%), Postives = 145/295 (49.15%), Query Frame = 0


Query: 27  SRDFAHRLGASEDLVLRLDLFRKLERHKGCVNTVSFSAAGDILVSGSDDRRVILWNWETG 86
           S +   R+     LV RL   ++LE H+GCVN +++++ G +L+SGSDD R+ +WN+ + 
Sbjct: 23  SHEVDQRMQFHSSLVRRLSQEQELEGHQGCVNALAWNSNGSLLISGSDDLRINIWNYSSR 82

Query: 87  HAKLCFQSGHNNNVFQAKIMPYTDDNSIVTCAADGQVRHAQIL--------DGGDVETAL 146
                  +GH  N+F  K +P T D  +V+ A D +VR             D   + +AL
Sbjct: 83  KLLHSIDTGHTANIFCTKFVPETSDELVVSGAGDAEVRLFNTSRLSGRAEDDNAIIPSAL 142

Query: 147 LGSHQGRAHKLAIEPGSPYIFYTCGEDGLVQHFDLRTR-----AAIELFTCQSV--DKRA 206
              H  R  KLA+EPG+P + ++  EDG ++  D R       A      C+SV  D R+
Sbjct: 143 YQCHTRRVKKLAVEPGNPNVVWSASEDGTLRQHDFRESTSCPPAGTAHQECRSVLLDLRS 202

Query: 207 GY--------MSSIQLNAIAIDPRNPNLFVVAGSDEYARLYDIRKYKGEGSTDFGQP--- 266
           G           ++ L +  I    P+L +V GSD +ARLYD R      S+    P   
Sbjct: 203 GAKRALADPPKQTLSLKSCDISATRPHLLLVGGSDAFARLYDRRMLPPLASSRKRMPPPP 262

Query: 267 -ADCYCPPHLI--GDEQVGITGLAFS-ELSELLVSYNDESIYLFNRDMGLGRSPY 292
             + +CP HL   G   + +T + FS    E+L+SY+ E +YL N + G G   Y
Sbjct: 263 CVNYFCPMHLSERGRTNLHLTHVTFSPNGEEVLLSYSGEHVYLMNVNNGTGIMQY 317

HSP 2 Score: 89.0 bits (219), Expect = 1.1e-17
Identity = 39/82 (47.56%), Postives = 58/82 (70.73%), Query Frame = 0


Query: 313 YKGHRNCET-VKGVNFFGPKCEYVISGSDCGRIFIWRKKGGGLIRVMEADEDVVNCIEPH 372
           Y GH N  T +K  +F G + EY+ SGSD GR FIW K+ G L++V+  DE V+NCI+ H
Sbjct: 610 YVGHCNVGTDIKQASFLGQRGEYIASGSDDGRWFIWEKQTGRLMKVLVGDESVLNCIQCH 669

Query: 373 PHMTMLASSGIESDVKLWTPKA 394
           P  +++A+SGI++ +K+W+P A
Sbjct: 670 PFDSVVATSGIDNTIKIWSPTA 691

The following BLAST results are available for this feature:

BLAST of Lag0001373 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022136068.1	9.0e-251	87.92	DDB1- and CUL4-associated factor 8 [Momordica charantia]	[more]
XP_038887987.1	1.0e-241	86.90	DDB1- and CUL4-associated factor 8-like [Benincasa hispida]	[more]
XP_008447917.1	2.2e-241	86.25	PREDICTED: DDB1- and CUL4-associated factor 8 [Cucumis melo]	[more]
TYK23393.1	9.4e-240	85.83	DDB1- and CUL4-associated factor 8 [Cucumis melo var. makuwa]	[more]
XP_004144864.1	1.4e-238	85.21	DDB1- and CUL4-associated factor 8 [Cucumis sativus] >KGN43277.1 hypothetical pr...	[more]

BLAST of Lag0001373 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8N7N5	5.6e-78	38.84	DDB1- and CUL4-associated factor 8 OS=Mus musculus OX=10090 GN=Dcaf8 PE=1 SV=1	[more]
Q5TAQ9	2.1e-77	38.63	DDB1- and CUL4-associated factor 8 OS=Homo sapiens OX=9606 GN=DCAF8 PE=1 SV=1	[more]
Q5U2M6	3.6e-77	38.41	DDB1- and CUL4-associated factor 8 OS=Rattus norvegicus OX=10116 GN=Dcaf8 PE=1 S...	[more]
Q6NRH1	4.7e-77	36.92	DDB1- and CUL4-associated factor 8 OS=Xenopus laevis OX=8355 GN=dcaf8 PE=2 SV=1	[more]
Q5R448	8.1e-77	38.63	DDB1- and CUL4-associated factor 8 OS=Pongo abelii OX=9601 GN=DCAF8 PE=2 SV=1	[more]

BLAST of Lag0001373 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C6L2	4.4e-251	87.92	DDB1- and CUL4-associated factor 8 OS=Momordica charantia OX=3673 GN=LOC11100785...	[more]
A0A1S3BIJ4	1.1e-241	86.25	DDB1- and CUL4-associated factor 8 OS=Cucumis melo OX=3656 GN=LOC103490261 PE=4 ...	[more]
A0A5D3DIH7	4.5e-240	85.83	DDB1-and CUL4-associated factor 8 OS=Cucumis melo var. makuwa OX=1194695 GN=E567...	[more]
A0A0A0K4R4	6.5e-239	85.21	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G016620 PE=4 SV=1	[more]
A0A6J1HVC3	1.2e-227	81.78	DDB1- and CUL4-associated factor 8-like OS=Cucurbita maxima OX=3661 GN=LOC111468...	[more]

BLAST of Lag0001373 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G35140.1	9.8e-187	64.91	Transducin/WD40 repeat-like superfamily protein	[more]
AT4G38480.1	6.6e-151	56.69	Transducin/WD40 repeat-like superfamily protein	[more]
AT3G45620.1	1.8e-119	48.47	Transducin/WD40 repeat-like superfamily protein	[more]
AT3G45620.2	1.5e-115	50.59	Transducin/WD40 repeat-like superfamily protein	[more]
AT5G10940.2	1.1e-36	32.20	transducin family protein / WD-40 repeat family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001680	WD40 repeat	SMART	SM00320	WD40_4	coord: 85..127 e-value: 15.0 score: 9.5 coord: 132..172 e-value: 1.6 score: 15.8 coord: 351..390 e-value: 0.044 score: 22.9 coord: 183..225 e-value: 11.0 score: 10.4 coord: 306..348 e-value: 1.4 score: 16.0 coord: 242..281 e-value: 81.0 score: 5.0 coord: 43..82 e-value: 1.6E-6 score: 37.7
IPR001680	WD40 repeat	PFAM	PF00400	WD40	coord: 47..82 e-value: 2.9E-5 score: 24.7 coord: 311..347 e-value: 0.16 score: 12.9
IPR001680	WD40 repeat	PROSITE	PS50082	WD_REPEATS_2	coord: 50..82 score: 13.248743
IPR015943	WD40/YVTN repeat-like-containing domain superfamily	GENE3D	2.130.10.10		coord: 38..392 e-value: 8.3E-49 score: 168.2
IPR045151	DDB1- and CUL4-associated factor 8-like	PANTHER	PTHR15574	WD REPEAT DOMAIN-CONTAINING FAMILY	coord: 8..465
None	No IPR available	PANTHER	PTHR15574:SF62	WD40/YVTN REPEAT-LIKE-CONTAINING DOMAIN-CONTAINING PROTEIN-RELATED	coord: 8..465
None	No IPR available	PROSITE	PS50294	WD_REPEATS_REGION	coord: 50..82 score: 10.996502
IPR036322	WD40-repeat-containing domain superfamily	SUPERFAMILY	50978	WD40 repeat-like	coord: 42..389

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0001373.1	Lag0001373.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0080008	Cul4-RING E3 ubiquitin ligase complex
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0005515	protein binding