Lag0001323 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0001323
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionO-methyltransferase family protein
Locationchr4: 29402366 .. 29404156 (+)
RNA-Seq ExpressionLag0001323
SyntenyLag0001323
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATATTGCTGGTAAAGATTCCAATTTCAAGAAAGAAGAAGATAGTGAAGTAAGGGTTCGAGTTTGGAAATACATATTTGGTTTCGTCGAAATGGCAGTAATAAAATGTGCCATAGAGCTTAAAATCGCAGATGCCATCGAAAGTCATGGAAGTCCCATAACACTGCCACAACTATCCTCTGCTACAAATTGCTCCGATCCCTCGCTCTTGCACCGCGTCATGCGCTTCTTGGTTCACCGTGGAATCTTCAAAGAAGAAACCGCAACTGACCATGGGTATGTTTGGTTTAACTTTTCAATCATTTAATTTTGAAAAAAAAAATCATTTCGAAATCTTTTTTAATGTTGGGTAACCACAAAAAATGGCTTATTTAATGAGATTAAGAAATAGATCATTTTGAGAAAAACACTTAAAAATGAATTTTAAAAAAGTAGGGGTATAAAAACACTTATGAGTAAAGGTAACCAAACATGTGAATGTTTAAATTCTAAACCATTTTTATACAAGATGTTTAAGAAAAAATGCATTCTTGAAAAACATTTAAACTAAAAGGCATTCCAAACGGACTCAGTCTCATAGGCTACGCTCAAACGGCCATGTCTCGCTTGCTCACCAGCGGCGGTGAGAACAGCCTAGCGCCCATACTTCTTTTGGAGGGCAGCCCAGTGATGCTGGCGCCATGGCATAATCTAAGTGCATTTCTCAAGGCGAATGACACTACACCATTCGAGATGGCTCATGGGAAGGACTTGTGGAGTTATGCTGCAGGCGATCCTTCACACAACATGTTGGTGAATGAGGGCATGGCTTGTTTTGCTAGGGTGGAAATTCTACCTGCGGTGCTTGAAAAGTGTGGAGAGATCTTTGAGGGAGTTGGATCTTTGGTGGATGTTGGAGGAGGAAATGGTACTTGTTTGAGCATTTTAGTGAAGGCTTGTCCTTGGATTAGAGGCATCAATTTTGATCTTCCACATGTTGTGTCTGTTTCACAAGAATACAAGGGCGTGCAACATATAGGTGGCAACATGTTCGATTGTATCCCAAAGGCCGATGCTGCTTTCATCATGGTTTGTTTATGCTATACCCTTGGACTATGAGATTGGTTTCAATTATACTCCTGAACGATCAAAAGTTTCATTTTAACCCTTCAAGTCTGGAGTTTGTTTCAAAACAACCCTTGAAGGCATTTTTTTTCGTTAAACTTATGGACGGAAAATGATGGGTTTGTATATGCGTATAATGCTTATATCCACGTATTTGAACATATACATGGAAATAACAATAAACACTCATGCAAACACATCATTTTTCGTCCATAAATTTAACGGAGATCTCCCAAGGTCACTTTGAAAGGGTTAAAATGAAACTTTTGAAAGTTCAGAGGTATAATTGAAACCAACCCCATAGTTTAGAGTTATTTTTTTGATAATTTAACCAATGCTAAAGTTAATAAACAACTTGTGTAGCTTTAACTTTGAGGTGAAAATGCAGGAGGTTCTACATGATTGGGATGATGAAGAATGCATCAAAATTTTGAAGAATTGCAAAGAAGCCATTCCAGAAGGGACAGGAAAAGTGATCATCGTTGAAGTGGTGATTGATAAAAAGGAAGAAAGCAAGTTGTCTGATGTAAGGTTGATGATGGATATGATAATGATGGCTCATACCACAAAGGGCAAAGAAAGAACACGTGAGGAATGGATCAATGTTCTTCAAAAGGCTGGTTTTAGTCGATATACCATTACTCCTACTCGAACCATCCAATCGGTCATTCAAGGGTTTCCTTGA

mRNA sequence

ATGGATATTGCTGGTAAAGATTCCAATTTCAAGAAAGAAGAAGATAGTGAAGTAAGGGTTCGAGTTTGGAAATACATATTTGGTTTCGTCGAAATGGCAGTAATAAAATGTGCCATAGAGCTTAAAATCGCAGATGCCATCGAAAGTCATGGAAGTCCCATAACACTGCCACAACTATCCTCTGCTACAAATTGCTCCGATCCCTCGCTCTTGCACCGCGTCATGCGCTTCTTGGTTCACCGTGGAATCTTCAAAGAAGAAACCGCAACTGACCATGGTCTCATAGGCTACGCTCAAACGGCCATGTCTCGCTTGCTCACCAGCGGCGGTGAGAACAGCCTAGCGCCCATACTTCTTTTGGAGGGCAGCCCAGTGATGCTGGCGCCATGGCATAATCTAAGTGCATTTCTCAAGGCGAATGACACTACACCATTCGAGATGGCTCATGGGAAGGACTTGTGGAGTTATGCTGCAGGCGATCCTTCACACAACATGTTGGTGAATGAGGGCATGGCTTGTTTTGCTAGGGTGGAAATTCTACCTGCGGTGCTTGAAAAGTGTGGAGAGATCTTTGAGGGAGTTGGATCTTTGGTGGATGTTGGAGGAGGAAATGGTACTTGTTTGAGCATTTTAGTGAAGGCTTGTCCTTGGATTAGAGGCATCAATTTTGATCTTCCACATGTTGTGTCTGTTTCACAAGAATACAAGGGCGTGCAACATATAGGTGGCAACATGTTCGATTGTATCCCAAAGGCCGATGCTGCTTTCATCATGGAGGTTCTACATGATTGGGATGATGAAGAATGCATCAAAATTTTGAAGAATTGCAAAGAAGCCATTCCAGAAGGGACAGGAAAAGTGATCATCGTTGAAGTGGTGATTGATAAAAAGGAAGAAAGCAAGTTGTCTGATGTAAGGTTGATGATGGATATGATAATGATGGCTCATACCACAAAGGGCAAAGAAAGAACACGTGAGGAATGGATCAATGTTCTTCAAAAGGCTGGTTTTAGTCGATATACCATTACTCCTACTCGAACCATCCAATCGGTCATTCAAGGGTTTCCTTGA

Coding sequence (CDS)

ATGGATATTGCTGGTAAAGATTCCAATTTCAAGAAAGAAGAAGATAGTGAAGTAAGGGTTCGAGTTTGGAAATACATATTTGGTTTCGTCGAAATGGCAGTAATAAAATGTGCCATAGAGCTTAAAATCGCAGATGCCATCGAAAGTCATGGAAGTCCCATAACACTGCCACAACTATCCTCTGCTACAAATTGCTCCGATCCCTCGCTCTTGCACCGCGTCATGCGCTTCTTGGTTCACCGTGGAATCTTCAAAGAAGAAACCGCAACTGACCATGGTCTCATAGGCTACGCTCAAACGGCCATGTCTCGCTTGCTCACCAGCGGCGGTGAGAACAGCCTAGCGCCCATACTTCTTTTGGAGGGCAGCCCAGTGATGCTGGCGCCATGGCATAATCTAAGTGCATTTCTCAAGGCGAATGACACTACACCATTCGAGATGGCTCATGGGAAGGACTTGTGGAGTTATGCTGCAGGCGATCCTTCACACAACATGTTGGTGAATGAGGGCATGGCTTGTTTTGCTAGGGTGGAAATTCTACCTGCGGTGCTTGAAAAGTGTGGAGAGATCTTTGAGGGAGTTGGATCTTTGGTGGATGTTGGAGGAGGAAATGGTACTTGTTTGAGCATTTTAGTGAAGGCTTGTCCTTGGATTAGAGGCATCAATTTTGATCTTCCACATGTTGTGTCTGTTTCACAAGAATACAAGGGCGTGCAACATATAGGTGGCAACATGTTCGATTGTATCCCAAAGGCCGATGCTGCTTTCATCATGGAGGTTCTACATGATTGGGATGATGAAGAATGCATCAAAATTTTGAAGAATTGCAAAGAAGCCATTCCAGAAGGGACAGGAAAAGTGATCATCGTTGAAGTGGTGATTGATAAAAAGGAAGAAAGCAAGTTGTCTGATGTAAGGTTGATGATGGATATGATAATGATGGCTCATACCACAAAGGGCAAAGAAAGAACACGTGAGGAATGGATCAATGTTCTTCAAAAGGCTGGTTTTAGTCGATATACCATTACTCCTACTCGAACCATCCAATCGGTCATTCAAGGGTTTCCTTGA

Protein sequence

MDIAGKDSNFKKEEDSEVRVRVWKYIFGFVEMAVIKCAIELKIADAIESHGSPITLPQLSSATNCSDPSLLHRVMRFLVHRGIFKEETATDHGLIGYAQTAMSRLLTSGGENSLAPILLLEGSPVMLAPWHNLSAFLKANDTTPFEMAHGKDLWSYAAGDPSHNMLVNEGMACFARVEILPAVLEKCGEIFEGVGSLVDVGGGNGTCLSILVKACPWIRGINFDLPHVVSVSQEYKGVQHIGGNMFDCIPKADAAFIMEVLHDWDDEECIKILKNCKEAIPEGTGKVIIVEVVIDKKEESKLSDVRLMMDMIMMAHTTKGKERTREEWINVLQKAGFSRYTITPTRTIQSVIQGFP
Homology
BLAST of Lag0001323 vs. NCBI nr
Match: XP_022135686.1 ((R,S)-reticuline 7-O-methyltransferase-like [Momordica charantia])

HSP 1 Score: 572.4 bits (1474), Expect = 2.7e-159
Identity = 279/349 (79.94%), Postives = 307/349 (87.97%), Query Frame = 0

Query: 8   SNFKKEEDSEVRVRVWKYIFGFVEMAVIKCAIELKIADAIESHGSPITLPQLSSATNCSD 67
           S  ++E+ SE R +VWKYIFGFVEMAVIKCAIELKI DAIESHGSPITLPQLSSA  CS 
Sbjct: 6   SKKEQEDHSEARTQVWKYIFGFVEMAVIKCAIELKIDDAIESHGSPITLPQLSSALKCSP 65

Query: 68  PSLLHRVMRFLVHRGIFKEETATDHGLIGYAQTAMSRLLTSGGENSLAPILLLEGSPVML 127
           PS LHRV+RFLVHRGIFKEE     G+ GY QT MSRLLTS  ++S+AP+LLLE +PVML
Sbjct: 66  PS-LHRVLRFLVHRGIFKEEETAIEGVKGYTQTPMSRLLTSSSKSSMAPLLLLESNPVML 125

Query: 128 APWHNLSAFLKANDTTPFEMAHGKDLWSYAAGDPSHNMLVNEGMACFARVEILPAVLEKC 187
           APWH LSAFLK +DT PFE AHG+DLWSYAA DPS  ML+NE MACFAR+ I+PAV+E+C
Sbjct: 126 APWHKLSAFLKDDDTPPFEAAHGRDLWSYAAADPSFTMLLNEAMACFARLVIVPAVIERC 185

Query: 188 GEIFEGVGSLVDVGGGNGTCLSILVKACPWIRGINFDLPHVVSVSQEYKGVQHIGGNMFD 247
           GEIFEGVGSLVDVGGGNGTCLSILVKA PWIRGINFDLPHVV VS  Y+GV+H+GGNMFD
Sbjct: 186 GEIFEGVGSLVDVGGGNGTCLSILVKAFPWIRGINFDLPHVVLVSPNYEGVKHVGGNMFD 245

Query: 248 CIPKADAAFIMEVLHDWDDEECIKILKNCKEAIPEGTGKVIIVEVVIDKKEESKLSDVRL 307
           C+PKADAAFIMEVLHDWDDEECIK+LKNCKEAIP+ TGKVIIVE+VID+KE++KL DVRL
Sbjct: 246 CVPKADAAFIMEVLHDWDDEECIKLLKNCKEAIPKKTGKVIIVEIVIDEKEQNKLLDVRL 305

Query: 308 MMDMIMMAHTTKGKERTREEWINVLQKAGFSRYTITPTRTIQSVIQGFP 357
           MMDMIMMAHT KGKERTREEW  VLQKAGFSRY ITPT TIQSVIQ FP
Sbjct: 306 MMDMIMMAHTNKGKERTREEWAYVLQKAGFSRYIITPTSTIQSVIQAFP 353

BLAST of Lag0001323 vs. NCBI nr
Match: XP_004144871.1 (acetylserotonin O-methyltransferase [Cucumis sativus] >KGN43290.1 hypothetical protein Csa_020497 [Cucumis sativus])

HSP 1 Score: 555.1 bits (1429), Expect = 4.5e-154
Identity = 278/362 (76.80%), Postives = 310/362 (85.64%), Query Frame = 0

Query: 2   DIAGKDSNFKK--EEDSEVRVRVWKYIFGFVEMAVIKCAIELKIADAIESHGSPITLPQL 61
           D + K SN  K  EEDS+++V++WKYIFGFVEMAVIKCAIELKI D IESHG  +TL QL
Sbjct: 3   DTSSKASNKLKIEEEDSDIKVQIWKYIFGFVEMAVIKCAIELKIGDVIESHGDSMTLSQL 62

Query: 62  SSATNCSDPSLLHRVMRFLVHRGIFKEETATDHGLIGYAQTAMSRLLTSGGENSLAPILL 121
           SS+ NCS P LLHR++RFLVHRGIFKEE  T      Y+ T MSRLLT+ G NS+AP+ L
Sbjct: 63  SSSLNCS-PLLLHRILRFLVHRGIFKEEKTTK----CYSHTPMSRLLTTTGPNSMAPLHL 122

Query: 122 LEGSPVMLAPWHNLSAFLKAND----TTPFEMAHGKDLWSYAAGDPSHNMLVNEGMACFA 181
           LE SPVM+APWHNLSA +KA+D    + PFEMAHG DLW+YAA +PSH+ L+NE MACFA
Sbjct: 123 LESSPVMIAPWHNLSACVKASDHENGSQPFEMAHGMDLWTYAAANPSHSSLINESMACFA 182

Query: 182 RVEILPAVLEKCGEIFEGVGSLVDVGGGNGTCLSILVKACPWIRGINFDLPHVVSVSQEY 241
           R+ ILPAVLE+CGEIF+GVG LVDVGGGNGTCLSILVKACPWI+GINFDLPHVVSVS+EY
Sbjct: 183 RLVILPAVLERCGEIFDGVGCLVDVGGGNGTCLSILVKACPWIKGINFDLPHVVSVSKEY 242

Query: 242 KGVQHIGGNMFDCIPKADAAFIMEVLHDWDDEECIKILKNCKEAIPEGTGKVIIVEVVID 301
           +G+QH+GGNMFD +PKADAAFIMEVLHDWDDEECIKILKNCKEAIPE TGKVIIVEVVID
Sbjct: 243 EGIQHVGGNMFDSVPKADAAFIMEVLHDWDDEECIKILKNCKEAIPEKTGKVIIVEVVID 302

Query: 302 KKEE-SKLSDVRLMMDMIMMAHTTKGKERTREEWINVLQKAGFSRYTITPTRTIQSVIQG 357
           +KEE SK SD RLMMDMIMMAHT KGKERT EEW NVLQKAGFS YTITPT TIQSVIQ 
Sbjct: 303 EKEEISKYSDARLMMDMIMMAHTIKGKERTNEEWANVLQKAGFSYYTITPTSTIQSVIQA 359

BLAST of Lag0001323 vs. NCBI nr
Match: XP_022969151.1 ((RS)-norcoclaurine 6-O-methyltransferase-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 547.4 bits (1409), Expect = 9.4e-152
Identity = 271/357 (75.91%), Postives = 312/357 (87.39%), Query Frame = 0

Query: 2   DIAGKDSNFKKEED-SEVRVRVWKYIFGFVEMAVIKCAIELKIADAIESHGSPITLPQLS 61
           +I  KDS FK+EE+ ++++++VWKYIFGFVEMAVIKCAIELKIAD I++HG  +TLP+LS
Sbjct: 3   NIGSKDSKFKREEEGNDIKIQVWKYIFGFVEMAVIKCAIELKIADTIDAHGGLMTLPELS 62

Query: 62  SATNCSDPSLLHRVMRFLVHRGIFKEETATDHGLIGYAQTAMSRLLTSGGENSLAPILLL 121
           S+ NCS P LL R++RFL+HRGIFKEE      +  YAQT MSRLLTS G +SLAPI +L
Sbjct: 63  SSLNCS-PLLLRRILRFLIHRGIFKEEP-----IKSYAQTPMSRLLTSSGAHSLAPIHVL 122

Query: 122 EGSPVMLAPWHNLSAFLKAND--TTPFEMAHGKDLWSYAAGDPSHNMLVNEGMACFARVE 181
           E S VMLAPWHNLSA+LKAN+  T PFEMAHGKDLWSY A + SHNM++NEGMA F+RV 
Sbjct: 123 ESSLVMLAPWHNLSAYLKANNGGTQPFEMAHGKDLWSYCAANSSHNMVMNEGMASFSRVV 182

Query: 182 ILPAVLEKCGEIFEGVGSLVDVGGGNGTCLSILVKACPWIRGINFDLPHVVSVSQEYKGV 241
           I+PAVLE+CG +FEGVGSLVDVGGGNG+CLS+LVKA PWIRGINFDLPHVVSVSQEY+GV
Sbjct: 183 IVPAVLERCGGVFEGVGSLVDVGGGNGSCLSVLVKAFPWIRGINFDLPHVVSVSQEYEGV 242

Query: 242 QHIGGNMFDCIPKADAAFIMEVLHDWDDEECIKILKNCKEAIPEGTGKVIIVEVVIDKKE 301
           +H+GGNMFD +PKADAAFIM  LHDWDDEECIKILKNCKEAIPE TGKVIIVEVVID+KE
Sbjct: 243 RHVGGNMFDFVPKADAAFIMNALHDWDDEECIKILKNCKEAIPEKTGKVIIVEVVIDEKE 302

Query: 302 ESKLSDVRLMMDMIMMAHTTKGKERTREEWINVLQKAGFSRYTITPTRTIQSVIQGF 356
           ES +SDVRLM+DMIM+AHT KGKERTREEW  VLQ AGFSR+TITPT TIQS+IQGF
Sbjct: 303 ESMVSDVRLMLDMIMIAHTIKGKERTREEWAYVLQGAGFSRHTITPTSTIQSIIQGF 353

BLAST of Lag0001323 vs. NCBI nr
Match: KAA0040348.1 ((RS)-norcoclaurine 6-O-methyltransferase-like [Cucumis melo var. makuwa] >TYK23356.1 (RS)-norcoclaurine 6-O-methyltransferase-like [Cucumis melo var. makuwa])

HSP 1 Score: 547.0 bits (1408), Expect = 1.2e-151
Identity = 273/363 (75.21%), Postives = 311/363 (85.67%), Query Frame = 0

Query: 2   DIAGKDSNFKK--EEDSEVRVRVWKYIFGFVEMAVIKCAIELKIADAIESHGSPITLPQL 61
           D   K+SN  K  EE+S+++V+VWKYIFGFVEMAV+KCAIELKI D IESHG  ITL QL
Sbjct: 3   DTGNKESNKLKIEEENSDIKVQVWKYIFGFVEMAVVKCAIELKIGDVIESHGDSITLSQL 62

Query: 62  SSATNCSDPSLLHRVMRFLVHRGIFKEETATDHGLIGYAQTAMSRLLTSGGENSLAPILL 121
           SS+ +CS P LLHR++RFLVHRGIFKEE ++      Y+ T MSRLLT+ G NS+API L
Sbjct: 63  SSSLSCS-PLLLHRILRFLVHRGIFKEEKSSK----SYSHTPMSRLLTTTGPNSMAPIHL 122

Query: 122 LEGSPVMLAPWHNLSAFLKA----NDTTPFEMAHGKDLWSYAAGDPSHNMLVNEGMACFA 181
           LE SPVM+APWHNLSA +KA    N + PFEMAHG DLW+YAA +PSH+ L+NEGMACFA
Sbjct: 123 LESSPVMIAPWHNLSACVKANGHENGSQPFEMAHGMDLWTYAAANPSHSSLLNEGMACFA 182

Query: 182 RVEILPAVLEKCGEIFEGVGSLVDVGGGNGTCLSILVKACPWIRGINFDLPHVVSVSQEY 241
           R+ ILPAVLE+CGEIF+GVG LVDVGGGNGTCLSILVKACPWI+GINFDLPHVVSVS+EY
Sbjct: 183 RLFILPAVLERCGEIFDGVGCLVDVGGGNGTCLSILVKACPWIKGINFDLPHVVSVSKEY 242

Query: 242 KGVQHIGGNMFDCIPKADAAFIMEVLHDWDDEECIKILKNCKEAIPEGTGKVIIVEVVID 301
           +GVQH+GG+MFD +PKADAAFIMEVLHDWDDEECIKIL+NCKEAIPE TGKVIIVE+VID
Sbjct: 243 EGVQHVGGDMFDSVPKADAAFIMEVLHDWDDEECIKILRNCKEAIPEKTGKVIIVEIVID 302

Query: 302 KKEE--SKLSDVRLMMDMIMMAHTTKGKERTREEWINVLQKAGFSRYTITPTRTIQSVIQ 357
           +KE+  SK SDV+L MDMIMMAHT KGKERT EEW N+LQKAGFS YTITPT TIQSVIQ
Sbjct: 303 EKEDQISKYSDVKLRMDMIMMAHTIKGKERTNEEWANLLQKAGFSYYTITPTTTIQSVIQ 360

BLAST of Lag0001323 vs. NCBI nr
Match: XP_008447905.1 (PREDICTED: (RS)-norcoclaurine 6-O-methyltransferase-like [Cucumis melo])

HSP 1 Score: 545.8 bits (1405), Expect = 2.7e-151
Identity = 272/363 (74.93%), Postives = 311/363 (85.67%), Query Frame = 0

Query: 2   DIAGKDSNFKK--EEDSEVRVRVWKYIFGFVEMAVIKCAIELKIADAIESHGSPITLPQL 61
           D   K+SN  K  EE+S+++V+VWKYIFGFVEMAV+KCAIELKI D IESHG  +TL QL
Sbjct: 3   DTGNKESNKLKIEEENSDIKVQVWKYIFGFVEMAVVKCAIELKIGDVIESHGDSMTLSQL 62

Query: 62  SSATNCSDPSLLHRVMRFLVHRGIFKEETATDHGLIGYAQTAMSRLLTSGGENSLAPILL 121
           SS+ +CS P LLHR++RFLVHRGIFKEE ++      Y+ T MSRLLT+ G NS+API L
Sbjct: 63  SSSLSCS-PLLLHRILRFLVHRGIFKEEKSSK----SYSHTPMSRLLTTTGPNSMAPIHL 122

Query: 122 LEGSPVMLAPWHNLSAFLKA----NDTTPFEMAHGKDLWSYAAGDPSHNMLVNEGMACFA 181
           LE SPVM+APWHNLSA +KA    N + PFEMAHG DLW+YAA +PSH+ L+NEGMACFA
Sbjct: 123 LESSPVMIAPWHNLSACVKANGHENGSQPFEMAHGMDLWTYAAANPSHSSLLNEGMACFA 182

Query: 182 RVEILPAVLEKCGEIFEGVGSLVDVGGGNGTCLSILVKACPWIRGINFDLPHVVSVSQEY 241
           R+ ILPAVLE+CGEIF+GVG LVDVGGGNGTCLSILVKACPWI+GINFDLPHVVSVS+EY
Sbjct: 183 RLFILPAVLERCGEIFDGVGCLVDVGGGNGTCLSILVKACPWIKGINFDLPHVVSVSKEY 242

Query: 242 KGVQHIGGNMFDCIPKADAAFIMEVLHDWDDEECIKILKNCKEAIPEGTGKVIIVEVVID 301
           +GVQH+GG+MFD +PKADAAFIMEVLHDWDDEECIKIL+NCKEAIPE TGKVIIVE+VID
Sbjct: 243 EGVQHVGGDMFDSVPKADAAFIMEVLHDWDDEECIKILRNCKEAIPEKTGKVIIVEIVID 302

Query: 302 KKEE--SKLSDVRLMMDMIMMAHTTKGKERTREEWINVLQKAGFSRYTITPTRTIQSVIQ 357
           +KE+  SK SDV+L MDMIMMAHT KGKERT EEW N+LQKAGFS YTITPT TIQSVIQ
Sbjct: 303 EKEDQISKYSDVKLRMDMIMMAHTIKGKERTNEEWANLLQKAGFSYYTITPTTTIQSVIQ 360

BLAST of Lag0001323 vs. ExPASy Swiss-Prot
Match: Q9T003 (Acetylserotonin O-methyltransferase OS=Arabidopsis thaliana OX=3702 GN=ASMT PE=1 SV=1)

HSP 1 Score: 347.1 bits (889), Expect = 2.4e-94
Identity = 179/357 (50.14%), Postives = 248/357 (69.47%), Query Frame = 0

Query: 13  EEDSEVRVRVWKYIFGFVEMAVIKCAIELKIADAIESHGS--PITLPQLSSATNCSDPSL 72
           +E+++  + +WKY+FGF ++A  KCAI+LKI +AIE+H S  P+TL +LSSA + S PS 
Sbjct: 24  DEEAKASLDIWKYVFGFADIAAAKCAIDLKIPEAIENHPSSQPVTLAELSSAVSAS-PSH 83

Query: 73  LHRVMRFLVHRGIFKEETATDHGLIGYAQTAMSR--LLTSGGENSLAPILLLEGSPVMLA 132
           L R+MRFLVH+GIFKE    D    GY  T +SR  ++T     SLAP +L E +P MLA
Sbjct: 84  LRRIMRFLVHQGIFKEIPTKDGLATGYVNTPLSRRLMITRRDGKSLAPFVLFETTPEMLA 143

Query: 133 PWHNLSAFLKA--NDTT--PFEMAHGKDLWSYAAGDPSHNMLVNEGMACFARVEILPAVL 192
           PW  LS+ + +  N +T  PF+  HGKD+WS+A  +P  + ++NE MAC AR  ++P V 
Sbjct: 144 PWLRLSSVVSSPVNGSTPPPFDAVHGKDVWSFAQDNPFLSDMINEAMACDAR-RVVPRVA 203

Query: 193 EKCGEIFEGVGSLVDVGGGNGTCLSILVKACPWIRGINFDLPHVVSVSQEYKGVQHIGGN 252
             C  +F+GV ++VDVGGG G  + +LVK  PWI+G NFDLPHV+ V++   GV+++ G+
Sbjct: 204 GACHGLFDGVTTMVDVGGGTGETMGMLVKEFPWIKGFNFDLPHVIEVAEVLDGVENVEGD 263

Query: 253 MFDCIPKADAAFIMEVLHDWDDEECIKILKNCKEAIPEGTGKVIIVE---------VVID 312
           MFD IP  DA FI  VLHDW D++CIKILKNCKEA+P   GKV+IVE         +++D
Sbjct: 264 MFDSIPACDAIFIKWVLHDWGDKDCIKILKNCKEAVPPNIGKVLIVESVIGENKKTMIVD 323

Query: 313 KKEESKLSDVRLMMDMIMMAHTTKGKERTREEWINVLQKAGFSRYTITPTRTIQSVI 353
           +++E KL  VRLM+DM+MMAHT+ GKERT +EW  VL++AGF+RY +     +QS+I
Sbjct: 324 ERDE-KLEHVRLMLDMVMMAHTSTGKERTLKEWDFVLKEAGFARYEVRDIDDVQSLI 377

BLAST of Lag0001323 vs. ExPASy Swiss-Prot
Match: Q6WUC2 ((R,S)-reticuline 7-O-methyltransferase OS=Papaver somniferum OX=3469 GN=7OMT PE=1 SV=1)

HSP 1 Score: 277.7 bits (709), Expect = 1.8e-73
Identity = 145/352 (41.19%), Postives = 212/352 (60.23%), Query Frame = 0

Query: 13  EEDSEVRVRVWKYIFGFVEMAVIKCAIELKIADAIESHGSPITLPQLSSATNCSDPS--- 72
           EE  + +  +W+++F FV+   +KCA+EL I D I SHG P+T+ ++  +   + PS   
Sbjct: 5   EERLKGQAEIWEHMFAFVDSMALKCAVELGIPDIINSHGRPVTISEIVDSLKTNTPSSSP 64

Query: 73  ---LLHRVMRFLVHRGIFKEETATDHGLIGYAQTAMSRLLTSGGENSLAPILLLEGSPVM 132
               L R+MR LVH+ +F  E   +   + Y  T  S+ L    + +L+P++L E +P++
Sbjct: 65  NIDYLTRIMRLLVHKRLFTSELHQESNQLLYNLTRSSKWLLKDSKFNLSPLVLWETNPIL 124

Query: 133 LAPWHNLSAFLKANDTTPFEMAHGKDLWSYAAGDPSHNMLVNEGMACFARVEILPAVLEK 192
           L PW  L    +   ++PFE AHG ++W  A  DP  N  +N  M C +   I+  +L +
Sbjct: 125 LKPWQYLGKCAQ-EKSSPFERAHGCEIWDLALADPKFNNFLNGAMQC-STTTIINEMLLE 184

Query: 193 CGEIFEGV-GSLVDVGGGNGTCLSILVKACPWIRGINFDLPHVVSVSQEYKGVQHIGGNM 252
             + F G+ GSLVDVGGG G+ ++ +VKA P I+GINFDLPHVV+ + E+ GV+H+GG+M
Sbjct: 185 YKDGFSGIAGSLVDVGGGTGSIIAEIVKAHPHIQGINFDLPHVVATAAEFPGVKHVGGDM 244

Query: 253 FDCIPKADAAFIMEVLHDWDDEECIKILKNCKEAI-PEGTGKVIIVEVVIDKKEESKLSD 312
           F  IP+ADA  +  +LHDW DE+C  ILKNC  AI  +  GKVIIV+ V+          
Sbjct: 245 FVDIPEADAVIMKWILHDWSDEDCTIILKNCYRAIRKKKNGKVIIVDCVLRPDGNDLFDK 304

Query: 313 VRLMMDMIMMAHTTKGKERTREEWINVLQKAGFSRYTITPTRTIQSVIQGFP 357
           + L+ D++MMAHTT GKERT  EW  +L  AGF RY +  T     +I+ FP
Sbjct: 305 MGLIFDVLMMAHTTAGKERTEAEWKILLNNAGFPRYNVIRTPAFPCIIEAFP 354

BLAST of Lag0001323 vs. ExPASy Swiss-Prot
Match: B0ZB56 (Xanthohumol 4-O-methyltransferase OS=Humulus lupulus OX=3486 GN=OMT2 PE=1 SV=1)

HSP 1 Score: 277.7 bits (709), Expect = 1.8e-73
Identity = 153/345 (44.35%), Postives = 216/345 (62.61%), Query Frame = 0

Query: 22  VWKYIFGFVEMAVIKCAIELKIADAIESHGSPITLPQLSSATNCSDPSL----LHRVMRF 81
           VWK I G  +  V+KCA+EL+I D + SH +PITL Q++S+   S PSL    L R+MR 
Sbjct: 19  VWKSINGIADFMVMKCALELRIPDIVHSHSAPITLAQIASSVPDS-PSLNLSYLSRIMRL 78

Query: 82  LVHRGIFKEETATDHGLIGYAQTAMSRLL----TSGGENSLAPILLLEGSPVMLAPWHNL 141
           LV R IF +  + D   + Y  T  SRLL    T   + +LAP +     P + APW  L
Sbjct: 79  LVRRKIFSQHKSLDGEEVLYGPTHSSRLLLSKTTLPDQVTLAPFVAFMTHPYLSAPWSCL 138

Query: 142 SAFLKANDTTPFEMAH-GKDLWSYAAGDPSHNMLVNEGMACFARVEILPAVLEKCGEIFE 201
           +  +K      FEM H G+ LW  + G+P  N + N+GMA  AR+  + A+L +  ++F 
Sbjct: 139 ARCVKEGG-NGFEMVHGGRQLWDLSPGNPEFNKVFNDGMASTARITTM-AILSEYRDVFC 198

Query: 202 GVGSLVDVGGGNGTCLSILVKACPWIRGINFDLPHVVSVSQEYKG-VQHIGGNMFDCIPK 261
           G+ SLVDVGG  G  +S +VK+ P I+GIN+DLPHVV+ +  Y G V H+GGNMF+ IP 
Sbjct: 199 GICSLVDVGGEFGGSISAIVKSHPHIKGINYDLPHVVATAPTYTGLVSHVGGNMFEWIPT 258

Query: 262 ADAAFIMEVLHDWDDEECIKILKNCKEAIPEGTGKVIIVEVVIDKKEESKLSDVRLMMDM 321
           A A F+  +LHDW DE+C+KILKNC+ A+PE  GK+IIV++V++ +      D  +M+D+
Sbjct: 259 AVAVFMKWILHDWADEDCVKILKNCRRAMPEKGGKIIIVDIVLEPEGNGLFDDAAVMLDI 318

Query: 322 IMMAHTTKGKERTREEWINVLQKAGFSRYTITPTRTIQSVIQGFP 357
            +MA  T+GKERT +EW  VL++ GF RY I     + SVI+ +P
Sbjct: 319 ALMA-LTRGKERTEKEWKRVLEEGGFPRYQILKIPALTSVIEAYP 359

BLAST of Lag0001323 vs. ExPASy Swiss-Prot
Match: Q7XB10 (3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase 2 OS=Papaver somniferum OX=3469 GN=4'OMT2 PE=1 SV=1)

HSP 1 Score: 271.6 bits (693), Expect = 1.3e-71
Identity = 137/341 (40.18%), Postives = 211/341 (61.88%), Query Frame = 0

Query: 19  RVRVWKYIFGFVEMAVIKCAIELKIADAIESHGSPITLPQLSS---ATNCSDPSLLHRVM 78
           + ++W  I+GF +  V++CA+E+ IAD I+++   ITL QL++    TN S    L+R++
Sbjct: 20  QAQLWNIIYGFADSLVLRCAVEIGIADIIKNNDGAITLAQLAAKLPITNVSS-DYLYRMV 79

Query: 79  RFLVHRGIFKEETATDHGLIGYAQTAMSRLLTSGGENSLAPILLLEGSPVMLAPWHNLSA 138
           R+LVH  I ++ET        Y+   +  LL    E S+ P++L       +  WH +  
Sbjct: 80  RYLVHLNIIEQETCNGGVEKVYSLKPVGTLLLRDAERSMVPMILGMTQKDFMVSWHFMKE 139

Query: 139 FLKANDTTPFEMAHGKDLWSYAAGDPSHNMLVNEGMACFARVEILPAVLEKCGEIFEGVG 198
            L    TT FE   G D+W Y  G+P  + L NEGMA   R+ +   ++E C + F+G+ 
Sbjct: 140 GLGNGSTTAFEKGMGMDIWKYLEGNPDQSQLFNEGMAGETRL-LTKTLIEDCRDTFQGLD 199

Query: 199 SLVDVGGGNGTCLSILVKACPWIRGINFDLPHVVSVSQEYKGVQHIGGNMFDCIPKADAA 258
           SLVD+GGGNGT +  + +A P I+   +DLPHVV+ S +   ++ + G+MF  +P A A 
Sbjct: 200 SLVDIGGGNGTTIKAIYEAFPHIKCTLYDLPHVVANSHDLPNIEKVPGDMFKSVPSAQAI 259

Query: 259 FIMEVLHDWDDEECIKILKNCKEAIPEGTGKVIIVEVVIDKKEESKLSDVRLMMDMIMMA 318
            +  +LHDW DEEC+ ILK CKEAIP+ TGKVIIV+V ++++   +L+  RL++D+ M+ 
Sbjct: 260 LLKLILHDWTDEECVNILKKCKEAIPKETGKVIIVDVALEEESNHELTKTRLILDIDMLV 319

Query: 319 HTTKGKERTREEWINVLQKAGFSRYTITPTRTIQSVIQGFP 357
           + T G+ERT ++W N+L++AGF  + I P R IQSVI+ FP
Sbjct: 320 N-TGGRERTADDWENLLKRAGFRSHKIRPIRAIQSVIEAFP 357

BLAST of Lag0001323 vs. ExPASy Swiss-Prot
Match: Q6WUC1 ((RS)-norcoclaurine 6-O-methyltransferase OS=Papaver somniferum OX=3469 GN=6OMT PE=1 SV=1)

HSP 1 Score: 267.7 bits (683), Expect = 1.9e-70
Identity = 143/346 (41.33%), Postives = 210/346 (60.69%), Query Frame = 0

Query: 15  DSEVRVRVWKYIFGFVEMAVIKCAIELKIADAIESHGSPITLPQLSSATNCSDP---SLL 74
           D + + ++WK I+GF E  V+KCA++L+IA+ + ++  P++L +L+S    + P     L
Sbjct: 8   DQQNQAKIWKQIYGFAESLVLKCAVQLEIAETLHNNVKPMSLSELASKLPVAQPVNEDRL 67

Query: 75  HRVMRFLVHRGIFKEETATDHGLIGYAQTAMSRLLTSGGENSLAPILLLEGSPVMLAPWH 134
            R+MR+LVH  +FK +  T      Y+    ++ L  G E S+   +L       LAPWH
Sbjct: 68  FRIMRYLVHMELFKIDATTQK----YSLAPPAKYLLRGWEKSMVDSILCINDKDFLAPWH 127

Query: 135 NLSAFLKANDTTPFEMAHGKDLWSYAAGDPSHNMLVNEGMACFARVEILPAVLEKCGEIF 194
           +L   L  N    FE A GK +W Y + +P  N L N  MAC  R+ +  A+  +C  IF
Sbjct: 128 HLGDGLTGN-CDAFEKALGKSIWVYMSVNPEKNQLFNAAMACDTRL-VTSALANECKSIF 187

Query: 195 -EGVGSLVDVGGGNGTCLSILVKACPWIRGINFDLPHVVSVSQEYKGVQHIGGNMFDCIP 254
            +G+ +LVDVGGG GT +  + KA P I+   +DLPHV++ S E   +  I G+MF  IP
Sbjct: 188 SDGISTLVDVGGGTGTAVKAISKAFPDIKCTIYDLPHVIADSPEIPNITKISGDMFKSIP 247

Query: 255 KADAAFIMEVLHDWDDEECIKILKNCKEAIPEGTGKVIIVEVVIDKKEESKLSDVRLMMD 314
            ADA F+  +LHDW+D+ECI+ILK CKEA+P+G GKVIIV+VVID       + +RL +D
Sbjct: 248 SADAIFMKCILHDWNDDECIQILKRCKEALPKG-GKVIIVDVVIDMDSTHPYAKIRLTLD 307

Query: 315 MIMMAHTTKGKERTREEWINVLQKAGFSRYTITPTRTIQSVIQGFP 357
           + MM + T GKERT+EEW  +   AGF+ + +T    +QSVI+ +P
Sbjct: 308 LDMMLN-TGGKERTKEEWKTLFDAAGFASHKVTQISAVQSVIEAYP 345

BLAST of Lag0001323 vs. ExPASy TrEMBL
Match: A0A6J1C1G3 ((R,S)-reticuline 7-O-methyltransferase-like OS=Momordica charantia OX=3673 GN=LOC111007584 PE=3 SV=1)

HSP 1 Score: 572.4 bits (1474), Expect = 1.3e-159
Identity = 279/349 (79.94%), Postives = 307/349 (87.97%), Query Frame = 0

Query: 8   SNFKKEEDSEVRVRVWKYIFGFVEMAVIKCAIELKIADAIESHGSPITLPQLSSATNCSD 67
           S  ++E+ SE R +VWKYIFGFVEMAVIKCAIELKI DAIESHGSPITLPQLSSA  CS 
Sbjct: 6   SKKEQEDHSEARTQVWKYIFGFVEMAVIKCAIELKIDDAIESHGSPITLPQLSSALKCSP 65

Query: 68  PSLLHRVMRFLVHRGIFKEETATDHGLIGYAQTAMSRLLTSGGENSLAPILLLEGSPVML 127
           PS LHRV+RFLVHRGIFKEE     G+ GY QT MSRLLTS  ++S+AP+LLLE +PVML
Sbjct: 66  PS-LHRVLRFLVHRGIFKEEETAIEGVKGYTQTPMSRLLTSSSKSSMAPLLLLESNPVML 125

Query: 128 APWHNLSAFLKANDTTPFEMAHGKDLWSYAAGDPSHNMLVNEGMACFARVEILPAVLEKC 187
           APWH LSAFLK +DT PFE AHG+DLWSYAA DPS  ML+NE MACFAR+ I+PAV+E+C
Sbjct: 126 APWHKLSAFLKDDDTPPFEAAHGRDLWSYAAADPSFTMLLNEAMACFARLVIVPAVIERC 185

Query: 188 GEIFEGVGSLVDVGGGNGTCLSILVKACPWIRGINFDLPHVVSVSQEYKGVQHIGGNMFD 247
           GEIFEGVGSLVDVGGGNGTCLSILVKA PWIRGINFDLPHVV VS  Y+GV+H+GGNMFD
Sbjct: 186 GEIFEGVGSLVDVGGGNGTCLSILVKAFPWIRGINFDLPHVVLVSPNYEGVKHVGGNMFD 245

Query: 248 CIPKADAAFIMEVLHDWDDEECIKILKNCKEAIPEGTGKVIIVEVVIDKKEESKLSDVRL 307
           C+PKADAAFIMEVLHDWDDEECIK+LKNCKEAIP+ TGKVIIVE+VID+KE++KL DVRL
Sbjct: 246 CVPKADAAFIMEVLHDWDDEECIKLLKNCKEAIPKKTGKVIIVEIVIDEKEQNKLLDVRL 305

Query: 308 MMDMIMMAHTTKGKERTREEWINVLQKAGFSRYTITPTRTIQSVIQGFP 357
           MMDMIMMAHT KGKERTREEW  VLQKAGFSRY ITPT TIQSVIQ FP
Sbjct: 306 MMDMIMMAHTNKGKERTREEWAYVLQKAGFSRYIITPTSTIQSVIQAFP 353

BLAST of Lag0001323 vs. ExPASy TrEMBL
Match: A0A0A0K0Q2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G018720 PE=3 SV=1)

HSP 1 Score: 555.1 bits (1429), Expect = 2.2e-154
Identity = 278/362 (76.80%), Postives = 310/362 (85.64%), Query Frame = 0

Query: 2   DIAGKDSNFKK--EEDSEVRVRVWKYIFGFVEMAVIKCAIELKIADAIESHGSPITLPQL 61
           D + K SN  K  EEDS+++V++WKYIFGFVEMAVIKCAIELKI D IESHG  +TL QL
Sbjct: 3   DTSSKASNKLKIEEEDSDIKVQIWKYIFGFVEMAVIKCAIELKIGDVIESHGDSMTLSQL 62

Query: 62  SSATNCSDPSLLHRVMRFLVHRGIFKEETATDHGLIGYAQTAMSRLLTSGGENSLAPILL 121
           SS+ NCS P LLHR++RFLVHRGIFKEE  T      Y+ T MSRLLT+ G NS+AP+ L
Sbjct: 63  SSSLNCS-PLLLHRILRFLVHRGIFKEEKTTK----CYSHTPMSRLLTTTGPNSMAPLHL 122

Query: 122 LEGSPVMLAPWHNLSAFLKAND----TTPFEMAHGKDLWSYAAGDPSHNMLVNEGMACFA 181
           LE SPVM+APWHNLSA +KA+D    + PFEMAHG DLW+YAA +PSH+ L+NE MACFA
Sbjct: 123 LESSPVMIAPWHNLSACVKASDHENGSQPFEMAHGMDLWTYAAANPSHSSLINESMACFA 182

Query: 182 RVEILPAVLEKCGEIFEGVGSLVDVGGGNGTCLSILVKACPWIRGINFDLPHVVSVSQEY 241
           R+ ILPAVLE+CGEIF+GVG LVDVGGGNGTCLSILVKACPWI+GINFDLPHVVSVS+EY
Sbjct: 183 RLVILPAVLERCGEIFDGVGCLVDVGGGNGTCLSILVKACPWIKGINFDLPHVVSVSKEY 242

Query: 242 KGVQHIGGNMFDCIPKADAAFIMEVLHDWDDEECIKILKNCKEAIPEGTGKVIIVEVVID 301
           +G+QH+GGNMFD +PKADAAFIMEVLHDWDDEECIKILKNCKEAIPE TGKVIIVEVVID
Sbjct: 243 EGIQHVGGNMFDSVPKADAAFIMEVLHDWDDEECIKILKNCKEAIPEKTGKVIIVEVVID 302

Query: 302 KKEE-SKLSDVRLMMDMIMMAHTTKGKERTREEWINVLQKAGFSRYTITPTRTIQSVIQG 357
           +KEE SK SD RLMMDMIMMAHT KGKERT EEW NVLQKAGFS YTITPT TIQSVIQ 
Sbjct: 303 EKEEISKYSDARLMMDMIMMAHTIKGKERTNEEWANVLQKAGFSYYTITPTSTIQSVIQA 359

BLAST of Lag0001323 vs. ExPASy TrEMBL
Match: A0A6J1HWX9 ((RS)-norcoclaurine 6-O-methyltransferase-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111468232 PE=3 SV=1)

HSP 1 Score: 547.4 bits (1409), Expect = 4.5e-152
Identity = 271/357 (75.91%), Postives = 312/357 (87.39%), Query Frame = 0

Query: 2   DIAGKDSNFKKEED-SEVRVRVWKYIFGFVEMAVIKCAIELKIADAIESHGSPITLPQLS 61
           +I  KDS FK+EE+ ++++++VWKYIFGFVEMAVIKCAIELKIAD I++HG  +TLP+LS
Sbjct: 3   NIGSKDSKFKREEEGNDIKIQVWKYIFGFVEMAVIKCAIELKIADTIDAHGGLMTLPELS 62

Query: 62  SATNCSDPSLLHRVMRFLVHRGIFKEETATDHGLIGYAQTAMSRLLTSGGENSLAPILLL 121
           S+ NCS P LL R++RFL+HRGIFKEE      +  YAQT MSRLLTS G +SLAPI +L
Sbjct: 63  SSLNCS-PLLLRRILRFLIHRGIFKEEP-----IKSYAQTPMSRLLTSSGAHSLAPIHVL 122

Query: 122 EGSPVMLAPWHNLSAFLKAND--TTPFEMAHGKDLWSYAAGDPSHNMLVNEGMACFARVE 181
           E S VMLAPWHNLSA+LKAN+  T PFEMAHGKDLWSY A + SHNM++NEGMA F+RV 
Sbjct: 123 ESSLVMLAPWHNLSAYLKANNGGTQPFEMAHGKDLWSYCAANSSHNMVMNEGMASFSRVV 182

Query: 182 ILPAVLEKCGEIFEGVGSLVDVGGGNGTCLSILVKACPWIRGINFDLPHVVSVSQEYKGV 241
           I+PAVLE+CG +FEGVGSLVDVGGGNG+CLS+LVKA PWIRGINFDLPHVVSVSQEY+GV
Sbjct: 183 IVPAVLERCGGVFEGVGSLVDVGGGNGSCLSVLVKAFPWIRGINFDLPHVVSVSQEYEGV 242

Query: 242 QHIGGNMFDCIPKADAAFIMEVLHDWDDEECIKILKNCKEAIPEGTGKVIIVEVVIDKKE 301
           +H+GGNMFD +PKADAAFIM  LHDWDDEECIKILKNCKEAIPE TGKVIIVEVVID+KE
Sbjct: 243 RHVGGNMFDFVPKADAAFIMNALHDWDDEECIKILKNCKEAIPEKTGKVIIVEVVIDEKE 302

Query: 302 ESKLSDVRLMMDMIMMAHTTKGKERTREEWINVLQKAGFSRYTITPTRTIQSVIQGF 356
           ES +SDVRLM+DMIM+AHT KGKERTREEW  VLQ AGFSR+TITPT TIQS+IQGF
Sbjct: 303 ESMVSDVRLMLDMIMIAHTIKGKERTREEWAYVLQGAGFSRHTITPTSTIQSIIQGF 353

BLAST of Lag0001323 vs. ExPASy TrEMBL
Match: A0A5D3DI94 ((RS)-norcoclaurine 6-O-methyltransferase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold142G004200 PE=3 SV=1)

HSP 1 Score: 547.0 bits (1408), Expect = 5.9e-152
Identity = 273/363 (75.21%), Postives = 311/363 (85.67%), Query Frame = 0

Query: 2   DIAGKDSNFKK--EEDSEVRVRVWKYIFGFVEMAVIKCAIELKIADAIESHGSPITLPQL 61
           D   K+SN  K  EE+S+++V+VWKYIFGFVEMAV+KCAIELKI D IESHG  ITL QL
Sbjct: 3   DTGNKESNKLKIEEENSDIKVQVWKYIFGFVEMAVVKCAIELKIGDVIESHGDSITLSQL 62

Query: 62  SSATNCSDPSLLHRVMRFLVHRGIFKEETATDHGLIGYAQTAMSRLLTSGGENSLAPILL 121
           SS+ +CS P LLHR++RFLVHRGIFKEE ++      Y+ T MSRLLT+ G NS+API L
Sbjct: 63  SSSLSCS-PLLLHRILRFLVHRGIFKEEKSSK----SYSHTPMSRLLTTTGPNSMAPIHL 122

Query: 122 LEGSPVMLAPWHNLSAFLKA----NDTTPFEMAHGKDLWSYAAGDPSHNMLVNEGMACFA 181
           LE SPVM+APWHNLSA +KA    N + PFEMAHG DLW+YAA +PSH+ L+NEGMACFA
Sbjct: 123 LESSPVMIAPWHNLSACVKANGHENGSQPFEMAHGMDLWTYAAANPSHSSLLNEGMACFA 182

Query: 182 RVEILPAVLEKCGEIFEGVGSLVDVGGGNGTCLSILVKACPWIRGINFDLPHVVSVSQEY 241
           R+ ILPAVLE+CGEIF+GVG LVDVGGGNGTCLSILVKACPWI+GINFDLPHVVSVS+EY
Sbjct: 183 RLFILPAVLERCGEIFDGVGCLVDVGGGNGTCLSILVKACPWIKGINFDLPHVVSVSKEY 242

Query: 242 KGVQHIGGNMFDCIPKADAAFIMEVLHDWDDEECIKILKNCKEAIPEGTGKVIIVEVVID 301
           +GVQH+GG+MFD +PKADAAFIMEVLHDWDDEECIKIL+NCKEAIPE TGKVIIVE+VID
Sbjct: 243 EGVQHVGGDMFDSVPKADAAFIMEVLHDWDDEECIKILRNCKEAIPEKTGKVIIVEIVID 302

Query: 302 KKEE--SKLSDVRLMMDMIMMAHTTKGKERTREEWINVLQKAGFSRYTITPTRTIQSVIQ 357
           +KE+  SK SDV+L MDMIMMAHT KGKERT EEW N+LQKAGFS YTITPT TIQSVIQ
Sbjct: 303 EKEDQISKYSDVKLRMDMIMMAHTIKGKERTNEEWANLLQKAGFSYYTITPTTTIQSVIQ 360

BLAST of Lag0001323 vs. ExPASy TrEMBL
Match: A0A1S3BHY1 ((RS)-norcoclaurine 6-O-methyltransferase-like OS=Cucumis melo OX=3656 GN=LOC103490251 PE=3 SV=1)

HSP 1 Score: 545.8 bits (1405), Expect = 1.3e-151
Identity = 272/363 (74.93%), Postives = 311/363 (85.67%), Query Frame = 0

Query: 2   DIAGKDSNFKK--EEDSEVRVRVWKYIFGFVEMAVIKCAIELKIADAIESHGSPITLPQL 61
           D   K+SN  K  EE+S+++V+VWKYIFGFVEMAV+KCAIELKI D IESHG  +TL QL
Sbjct: 3   DTGNKESNKLKIEEENSDIKVQVWKYIFGFVEMAVVKCAIELKIGDVIESHGDSMTLSQL 62

Query: 62  SSATNCSDPSLLHRVMRFLVHRGIFKEETATDHGLIGYAQTAMSRLLTSGGENSLAPILL 121
           SS+ +CS P LLHR++RFLVHRGIFKEE ++      Y+ T MSRLLT+ G NS+API L
Sbjct: 63  SSSLSCS-PLLLHRILRFLVHRGIFKEEKSSK----SYSHTPMSRLLTTTGPNSMAPIHL 122

Query: 122 LEGSPVMLAPWHNLSAFLKA----NDTTPFEMAHGKDLWSYAAGDPSHNMLVNEGMACFA 181
           LE SPVM+APWHNLSA +KA    N + PFEMAHG DLW+YAA +PSH+ L+NEGMACFA
Sbjct: 123 LESSPVMIAPWHNLSACVKANGHENGSQPFEMAHGMDLWTYAAANPSHSSLLNEGMACFA 182

Query: 182 RVEILPAVLEKCGEIFEGVGSLVDVGGGNGTCLSILVKACPWIRGINFDLPHVVSVSQEY 241
           R+ ILPAVLE+CGEIF+GVG LVDVGGGNGTCLSILVKACPWI+GINFDLPHVVSVS+EY
Sbjct: 183 RLFILPAVLERCGEIFDGVGCLVDVGGGNGTCLSILVKACPWIKGINFDLPHVVSVSKEY 242

Query: 242 KGVQHIGGNMFDCIPKADAAFIMEVLHDWDDEECIKILKNCKEAIPEGTGKVIIVEVVID 301
           +GVQH+GG+MFD +PKADAAFIMEVLHDWDDEECIKIL+NCKEAIPE TGKVIIVE+VID
Sbjct: 243 EGVQHVGGDMFDSVPKADAAFIMEVLHDWDDEECIKILRNCKEAIPEKTGKVIIVEIVID 302

Query: 302 KKEE--SKLSDVRLMMDMIMMAHTTKGKERTREEWINVLQKAGFSRYTITPTRTIQSVIQ 357
           +KE+  SK SDV+L MDMIMMAHT KGKERT EEW N+LQKAGFS YTITPT TIQSVIQ
Sbjct: 303 EKEDQISKYSDVKLRMDMIMMAHTIKGKERTNEEWANLLQKAGFSYYTITPTTTIQSVIQ 360

BLAST of Lag0001323 vs. TAIR 10
Match: AT4G35160.1 (O-methyltransferase family protein )

HSP 1 Score: 347.1 bits (889), Expect = 1.7e-95
Identity = 179/357 (50.14%), Postives = 248/357 (69.47%), Query Frame = 0

Query: 13  EEDSEVRVRVWKYIFGFVEMAVIKCAIELKIADAIESHGS--PITLPQLSSATNCSDPSL 72
           +E+++  + +WKY+FGF ++A  KCAI+LKI +AIE+H S  P+TL +LSSA + S PS 
Sbjct: 24  DEEAKASLDIWKYVFGFADIAAAKCAIDLKIPEAIENHPSSQPVTLAELSSAVSAS-PSH 83

Query: 73  LHRVMRFLVHRGIFKEETATDHGLIGYAQTAMSR--LLTSGGENSLAPILLLEGSPVMLA 132
           L R+MRFLVH+GIFKE    D    GY  T +SR  ++T     SLAP +L E +P MLA
Sbjct: 84  LRRIMRFLVHQGIFKEIPTKDGLATGYVNTPLSRRLMITRRDGKSLAPFVLFETTPEMLA 143

Query: 133 PWHNLSAFLKA--NDTT--PFEMAHGKDLWSYAAGDPSHNMLVNEGMACFARVEILPAVL 192
           PW  LS+ + +  N +T  PF+  HGKD+WS+A  +P  + ++NE MAC AR  ++P V 
Sbjct: 144 PWLRLSSVVSSPVNGSTPPPFDAVHGKDVWSFAQDNPFLSDMINEAMACDAR-RVVPRVA 203

Query: 193 EKCGEIFEGVGSLVDVGGGNGTCLSILVKACPWIRGINFDLPHVVSVSQEYKGVQHIGGN 252
             C  +F+GV ++VDVGGG G  + +LVK  PWI+G NFDLPHV+ V++   GV+++ G+
Sbjct: 204 GACHGLFDGVTTMVDVGGGTGETMGMLVKEFPWIKGFNFDLPHVIEVAEVLDGVENVEGD 263

Query: 253 MFDCIPKADAAFIMEVLHDWDDEECIKILKNCKEAIPEGTGKVIIVE---------VVID 312
           MFD IP  DA FI  VLHDW D++CIKILKNCKEA+P   GKV+IVE         +++D
Sbjct: 264 MFDSIPACDAIFIKWVLHDWGDKDCIKILKNCKEAVPPNIGKVLIVESVIGENKKTMIVD 323

Query: 313 KKEESKLSDVRLMMDMIMMAHTTKGKERTREEWINVLQKAGFSRYTITPTRTIQSVI 353
           +++E KL  VRLM+DM+MMAHT+ GKERT +EW  VL++AGF+RY +     +QS+I
Sbjct: 324 ERDE-KLEHVRLMLDMVMMAHTSTGKERTLKEWDFVLKEAGFARYEVRDIDDVQSLI 377

BLAST of Lag0001323 vs. TAIR 10
Match: AT4G35150.1 (O-methyltransferase family protein )

HSP 1 Score: 300.1 bits (767), Expect = 2.4e-81
Identity = 158/350 (45.14%), Postives = 215/350 (61.43%), Query Frame = 0

Query: 13  EEDSEVRVRVWKYIFGFVEMAVIKCAIELKIADAIESHGS--PITLPQLSSATNCSDPSL 72
           +E+++  + +W+Y+FGF ++A  KCAI+LKI +AIE+H S  P+TL +LSSA + S PS 
Sbjct: 10  DEEAKASLDIWRYVFGFADIAAAKCAIDLKIPEAIENHPSSQPVTLSELSSAVSAS-PSH 69

Query: 73  LHRVMRFLVHRGIFKEETATDHGLIGYAQTAMSRLLTSGGENSLAPILLLEGSPVMLAPW 132
           L R+MRFLVH+G+FKE    D    GY  T +SR +                        
Sbjct: 70  LRRIMRFLVHQGLFKEVPTKDGLATGYTNTPLSRRMM----------------------- 129

Query: 133 HNLSAFLKANDTTPFEMAHGKDLWSYAAGDPSHNMLVNEGMACFARVEILPAVLEKCGEI 192
                             HGKDLW++A  +  H+ L+NE MAC AR  ++P V   C  +
Sbjct: 130 --------------ITKLHGKDLWAFAQDNLCHSQLINEAMACDAR-RVVPRVAGACQGL 189

Query: 193 FEGVGSLVDVGGGNGTCLSILVKACPWIRGINFDLPHVVSVSQEYKGVQHIGGNMFDCIP 252
           F+GV ++VDVGGG G  + ILVK  PWI+G NFDLPHV+ V+Q   GV+++ G+MFD IP
Sbjct: 190 FDGVATVVDVGGGTGETMGILVKEFPWIKGFNFDLPHVIEVAQVLDGVENVEGDMFDSIP 249

Query: 253 KADAAFIMEVLHDWDDEECIKILKNCKEAIPEGTGKVIIVEVVIDKKE--------ESKL 312
            +DA  I  VLHDW D++CIKILKNCKEA+    GKV+IVE VI +K+        + KL
Sbjct: 250 ASDAVIIKWVLHDWGDKDCIKILKNCKEAVLPNIGKVLIVECVIGEKKNTMIAEERDDKL 309

Query: 313 SDVRLMMDMIMMAHTTKGKERTREEWINVLQKAGFSRYTITPTRTIQSVI 353
             VRL +DM+MM HT+ GKERT +EW  VL +AGF+RY +     +QS+I
Sbjct: 310 EHVRLQLDMVMMVHTSTGKERTLKEWDFVLTEAGFARYEVRDFDDVQSLI 320

BLAST of Lag0001323 vs. TAIR 10
Match: AT5G54160.1 (O-methyltransferase 1 )

HSP 1 Score: 190.3 bits (482), Expect = 2.7e-48
Identity = 109/314 (34.71%), Postives = 180/314 (57.32%), Query Frame = 0

Query: 35  IKCAIELKIADAIESHGSPITLPQLSS---ATNCSDPSLLHRVMRFLVHRGIFKEETATD 94
           +K A+EL + + +  +GSP++  +++S     N   P +L R++R L    +    T ++
Sbjct: 36  LKSALELDLLEIMAKNGSPMSPTEIASKLPTKNPEAPVMLDRILRLLTSYSVL---TCSN 95

Query: 95  HGLIG------YAQTAMSRLLTSGGEN-SLAPILLLEGSPVMLAPWHNLSAFLKANDTTP 154
             L G      Y    + + LT   +  S+A + L+    V++  W++L   +  +   P
Sbjct: 96  RKLSGDGVERIYGLGPVCKYLTKNEDGVSIAALCLMNQDKVLMESWYHLKDAI-LDGGIP 155

Query: 155 FEMAHGKDLWSYAAGDPSHNMLVNEGMACFARVEILPAVLEKCGEIFEGVGSLVDVGGGN 214
           F  A+G   + Y   DP  N + N GM+  + +  +  +LE   + FEG+ SLVDVGGG 
Sbjct: 156 FNKAYGMSAFEYHGTDPRFNKVFNNGMSNHSTI-TMKKILETY-KGFEGLTSLVDVGGGI 215

Query: 215 GTCLSILVKACPWIRGINFDLPHVVSVSQEYKGVQHIGGNMFDCIPKADAAFIMEVLHDW 274
           G  L ++V   P ++GINFDLPHV+  +  + G++H+GG+MF  +PK DA F+  + HDW
Sbjct: 216 GATLKMIVSKYPNLKGINFDLPHVIEDAPSHPGIEHVGGDMFVSVPKGDAIFMKWICHDW 275

Query: 275 DDEECIKILKNCKEAIPEGTGKVIIVEVVIDKKEESKLSDVRLM-MDMIMMAHTTKGKER 334
            DE C+K LKNC E++PE  GKVI+ E ++ +  +S LS  +++ +D IM+AH   GKER
Sbjct: 276 SDEHCVKFLKNCYESLPE-DGKVILAECILPETPDSSLSTKQVVHVDCIMLAHNPGGKER 335

Query: 335 TREEWINVLQKAGF 338
           T +E+  + + +GF
Sbjct: 336 TEKEFEALAKASGF 342

BLAST of Lag0001323 vs. TAIR 10
Match: AT1G77520.1 (O-methyltransferase family protein )

HSP 1 Score: 177.9 bits (450), Expect = 1.4e-44
Identity = 125/343 (36.44%), Postives = 177/343 (51.60%), Query Frame = 0

Query: 34  VIKCAIELKIADAIESHGSPITLPQLSSA-------TNCSDPSLLHRVMRFLVHRGIFK- 93
           V+K A EL + D I + G+   L     A       TN   P LL R++  LV   I K 
Sbjct: 45  VLKAAFELGVIDTIAAAGNDTWLSPCEIACSLPTKPTNPEAPVLLDRMLSLLVSHSILKC 104

Query: 94  --EETATDHGLIG-----YAQTAMSR--LLTSGGENSLAPILLLEGSPVMLAPWHNL-SA 153
              ET  ++G  G     YA   + +  L  S G  SL P+ +L  + V    W NL   
Sbjct: 105 RMIETG-ENGRTGKIERVYAAEPVCKYFLRDSDGTGSLVPLFMLLHTQVFFKTWTNLKDV 164

Query: 154 FLKANDTTPFEMAHGKDLWSYAAGDPSHNMLVNEGMACFARVEILPAVLEKCGEI---FE 213
            L+  D   F  AHG  ++ Y   D     L N  M+     E    +++K  ++   FE
Sbjct: 165 ILEGRDA--FNSAHGMKIFEYINSDQPFAELFNRAMS-----EPSTMIMKKVLDVYRGFE 224

Query: 214 GVGSLVDVGGGNGTCLSILVKACPWIRGINFDLPHVVSVSQEYKGVQHIGGNMFDCIPKA 273
            V +LVDVGGGNGT L ++    P I+G+NFDL  V++ +  Y GV+H+ G+MF  +PK 
Sbjct: 225 DVNTLVDVGGGNGTVLGLVTSKYPHIKGVNFDLAQVLTQAPFYPGVEHVSGDMFVEVPKG 284

Query: 274 DAAFIMEVLHDWDDEECIKILKNCKEAIPEGTGKVIIVEVVIDK--KEESKLSDVRLMMD 333
           DA F+  +LHDW DE+CIKILKNC +++PE  GK+IIVE V  K  K     S+    MD
Sbjct: 285 DAVFMKWILHDWGDEDCIKILKNCWKSLPE-KGKIIIVEFVTPKEPKGGDLSSNTVFAMD 344

Query: 334 MIMMAHTTKGKERTREEWINVLQKAGFSRYTITPTRTIQSVIQ 354
           ++M+   + GKER+  ++ N+   +GF R  I       SVI+
Sbjct: 345 LLMLTQCSGGKERSLSQFENLAFASGFLRCEIICLAYSYSVIE 378

BLAST of Lag0001323 vs. TAIR 10
Match: AT1G77530.1 (O-methyltransferase family protein )

HSP 1 Score: 177.2 bits (448), Expect = 2.4e-44
Identity = 121/323 (37.46%), Postives = 179/323 (55.42%), Query Frame = 0

Query: 34  VIKCAIELKIADAI--ESHG-----SPITLPQLSSATNCSDPSLLHRVMRFLVHRGIFK- 93
           V+K A+EL + D I   S+G     S I +   +  TN   P LL R++R LV   I K 
Sbjct: 45  VLKAALELGVIDTIAAASNGTWLSPSEIAVSLPNKPTNPEAPVLLDRMLRLLVSHSILKC 104

Query: 94  -EETATDHGLIG-----YAQTAMSR--LLTSGGENSLAPILLLEGSPVMLAPWHNL-SAF 153
               + ++G  G     YA   + +  L  S G  SL+ +LLL  S V+L  W NL    
Sbjct: 105 CMVESRENGQTGKIERVYAAEPICKYFLKDSDGSGSLSSLLLLLHSQVILKTWTNLKDVI 164

Query: 154 LKANDTTPFEMAHGKDLWSYAAGDPSHNMLVNEGMACFARVEILPAVLEKCGEIFEGVGS 213
           L+  D   F  AH   L+ Y + D   + L +  M+  +   ++  VLE+    FE V +
Sbjct: 165 LEGKDA--FSSAHDMRLFEYISSDDQFSKLFHRAMS-ESSTMVMKKVLEEY-RGFEDVNT 224

Query: 214 LVDVGGGNGTCLSILVKACPWIRGINFDLPHVVSVSQEYKGVQHIGGNMFDCIPKADAAF 273
           LVDVGGG GT L ++    P I+G+NFDL  V++ +  Y GV+H+ G+MF  +PK DA F
Sbjct: 225 LVDVGGGIGTILGLITSKYPHIKGVNFDLAQVLTQAPFYPGVKHVSGDMFIEVPKGDAIF 284

Query: 274 IMEVLHDWDDEECIKILKNCKEAIPEGTGKVIIVEVV--IDKKEESKLSDVRLMMDMIMM 333
           +  +LHDW DE+CIKILKNC +++PE  GKVIIVE++  ++ K      +  L MD++M+
Sbjct: 285 MKWILHDWGDEDCIKILKNCWKSLPE-KGKVIIVEMITPMEPKPNDFSCNTVLGMDLLML 344

Query: 334 AHTTKGKERTREEWINVLQKAGF 338
              + GKER+  ++ N+   +GF
Sbjct: 345 TQCSGGKERSLSQFENLAFASGF 362

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022135686.12.7e-15979.94(R,S)-reticuline 7-O-methyltransferase-like [Momordica charantia][more]
XP_004144871.14.5e-15476.80acetylserotonin O-methyltransferase [Cucumis sativus] >KGN43290.1 hypothetical p... [more]
XP_022969151.19.4e-15275.91(RS)-norcoclaurine 6-O-methyltransferase-like isoform X1 [Cucurbita maxima][more]
KAA0040348.11.2e-15175.21(RS)-norcoclaurine 6-O-methyltransferase-like [Cucumis melo var. makuwa] >TYK233... [more]
XP_008447905.12.7e-15174.93PREDICTED: (RS)-norcoclaurine 6-O-methyltransferase-like [Cucumis melo][more]
Match NameE-valueIdentityDescription
Q9T0032.4e-9450.14Acetylserotonin O-methyltransferase OS=Arabidopsis thaliana OX=3702 GN=ASMT PE=1... [more]
Q6WUC21.8e-7341.19(R,S)-reticuline 7-O-methyltransferase OS=Papaver somniferum OX=3469 GN=7OMT PE=... [more]
B0ZB561.8e-7344.35Xanthohumol 4-O-methyltransferase OS=Humulus lupulus OX=3486 GN=OMT2 PE=1 SV=1[more]
Q7XB101.3e-7140.183'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase 2 OS=Papaver somniferu... [more]
Q6WUC11.9e-7041.33(RS)-norcoclaurine 6-O-methyltransferase OS=Papaver somniferum OX=3469 GN=6OMT P... [more]
Match NameE-valueIdentityDescription
A0A6J1C1G31.3e-15979.94(R,S)-reticuline 7-O-methyltransferase-like OS=Momordica charantia OX=3673 GN=LO... [more]
A0A0A0K0Q22.2e-15476.80Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G018720 PE=3 SV=1[more]
A0A6J1HWX94.5e-15275.91(RS)-norcoclaurine 6-O-methyltransferase-like isoform X1 OS=Cucurbita maxima OX=... [more]
A0A5D3DI945.9e-15275.21(RS)-norcoclaurine 6-O-methyltransferase-like OS=Cucumis melo var. makuwa OX=119... [more]
A0A1S3BHY11.3e-15174.93(RS)-norcoclaurine 6-O-methyltransferase-like OS=Cucumis melo OX=3656 GN=LOC1034... [more]
Match NameE-valueIdentityDescription
AT4G35160.11.7e-9550.14O-methyltransferase family protein [more]
AT4G35150.12.4e-8145.14O-methyltransferase family protein [more]
AT5G54160.12.7e-4834.71O-methyltransferase 1 [more]
AT1G77520.11.4e-4436.44O-methyltransferase family protein [more]
AT1G77530.12.4e-4437.46O-methyltransferase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012967Plant methyltransferase dimerisationPFAMPF08100Dimerisationcoord: 34..79
e-value: 6.5E-10
score: 38.9
IPR036388Winged helix-like DNA-binding domain superfamilyGENE3D1.10.10.10coord: 4..106
e-value: 4.9E-27
score: 95.5
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 108..354
e-value: 1.2E-77
score: 262.3
NoneNo IPR availablePIRSRPIRSR005739-1PIRSR005739-1coord: 33..353
e-value: 7.5E-87
score: 288.9
NoneNo IPR availablePANTHERPTHR11746:SF1913'-HYDROXY-N-METHYL-(S)-COCLAURINE 4'-O-METHYLTRANSFERASE 2-LIKEcoord: 8..346
IPR016461O-methyltransferase COMT-typePIRSFPIRSF005739O-mtasecoord: 1..356
e-value: 4.5E-118
score: 392.2
IPR016461O-methyltransferase COMT-typePANTHERPTHR11746O-METHYLTRANSFERASEcoord: 8..346
IPR016461O-methyltransferase COMT-typePROSITEPS51683SAM_OMT_IIcoord: 23..356
score: 75.69416
IPR001077O-methyltransferase domainPFAMPF00891Methyltransf_2coord: 130..338
e-value: 4.0E-53
score: 179.9
IPR036390Winged helix DNA-binding domain superfamilySUPERFAMILY46785"Winged helix" DNA-binding domaincoord: 13..109
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 113..352

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0001323.1Lag0001323.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0019438 aromatic compound biosynthetic process
biological_process GO:0032259 methylation
molecular_function GO:0008171 O-methyltransferase activity
molecular_function GO:0046983 protein dimerization activity
molecular_function GO:0008757 S-adenosylmethionine-dependent methyltransferase activity
molecular_function GO:0008168 methyltransferase activity