Lag0001315 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0001315
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionL-ascorbate oxidase homolog
Locationchr4: 29257516 .. 29259526 (+)
RNA-Seq ExpressionLag0001315
SyntenyLag0001315
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCATTAAGATCATCACCTAAGTTTTGGTGTTTTCATGTTGCTCTGCTTTTGGGAATGCTGGCCATTGCCGTTGCCGAAAACCCGTATCGATTCTTCGATTGGAATGTCACCTATGGCGATATTTACCCGCTCGCCATTCGTCAACAGGTAGGGATTGAATTTATATCCTAGTATGTTGGTCTAAGCTTTTGAGTTCAGTGGTGATTTAATGTCGTGTTTTTTTTTAGGGGATTTTGATAAATGGGCAATTTCCGGGACCGGATATCTATGCGGTTACGAACGATAATCTTTACATCAATGTTCATAATAGTCTTCCGGAGCCATTTCTTCTGTCTTGGTCAGTGTTCTCTTTCTCTCTCTATTTTCTCTCTCTTTCTTTACCGGGTGGAATAGTCTTGTTTTGAAGGAAGGAATGTTGGCAGTAAGATTATTGTTTGAATTTTTGAAGAAACGAATGATATCATTCCTAATCCATTTTTAAAGGTATTTTAAAATTTTAAGTAAATATTCATTTAGTGAAAAGAAAAAAGCTTTTGGAAAGTATTTTGTTTTTGAAATGTGGGGAAGAATTTTAAAAGTATTCTTTTGGAAGTGTATAAAGGAAATAATAGTAATTAGGAGGGAATTAGGCCTAATCGTTAAAAATAAAATCTAAAAATGGAATGGCAGGAATATTTAGAAAAGAGGAACAAATGTTTCTAGATGATATAATGTTTTAACAAATTGAATTTATAACTAATTAAGTTGATAATATTGGAGTTATATAGAAATGAGAAATTTATTTAATTTCTAATCTAGGTATTTTCATTTGATTTCTTTATTAATCCTATTATAATACATAAGTTAAAATTGCAAATTTGATATTCCAAACAGCACGTGAGAAGAGAATAAATGCAATTTTAACTCTAAACTCCAAAGTCGCTTTAAAGCTTTATTAATTTGGCTTGTGAAAAACGTAACCAAGGTATGGAGTGACGTTATCAACCACCCAACTCCCAACAACACGTAAACCAAAAAAAAAAGCAATTGGACCTCAAGCCCATTGAAGTTGGGCCTCCGGTTTTAATTTCGGCCCATTAAATATTAACCGCTCGTTTTTCATTTTTTTTTGTTAATTAAATGCAGGAACGGAATTCAACAGAGGAAAAACTCGTACCAAGACGGAGTGTACGGAACAACGTGCCCAATTCCGCCGGGCAAGAACTTCACCTACAGAATTCAGGTGAAGGATCAGATCGGAAGTTACTTCTATTTCCCCTCTCTTACATTCCACAAGGCCGCCGGTGGATTCGGGAGTATCAGAGTCCTCAGCCGTCCCCAAATTCCGGTCCCATTCCCGGAGCCCGCCGCCGATTACACCCTTCTCGTCGGTGACTGGTACACCGCCAATCACACGGTAACTACCGATTTAACTTTCGAGCCTCTGAATTATGTACAGGTTTGGATGATTTGATTTGATGGAATATGTTTCTGATTTCGAGTGTTTGCGTTGTTTGTTGTTTGTTTTGATCAGACATTGAAGACCATCTTGGATCGTGGTCACAGACTCCCTTTTCCCAATGGAATTGTTATCAATGGCCGTGGTCCGAACGCTACCACTTTCACCGTCGAAAAGGGTAATTTGAGATTTGGACTCGTTTTTTATGTATCTTGTGGAAGAATGGGGTAATTTGAAGTGAAATTTATGGGTGTGTTTATTTTTTTTGCAGGTAAGACTTACAGGTTTAGAATCTCGAATGTGGGGCTCCAGCATTCGCTCAATTTCAGGGTTCAAGATCATGAGATGAAGCTTGTTGAAGTTGAAGGCACTCACACAATGCAGAATACTTATTCGTCGCTTGATGTTCATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATGCAATCTTGAGCAAGTCTAGATCAAATCCCCTCTCCCGAGCTAGATTCGAAACTTAA

mRNA sequence

ATGGCATTAAGATCATCACCTAAGTTTTGGTGTTTTCATGTTGCTCTGCTTTTGGGAATGCTGGCCATTGCCGTTGCCGAAAACCCGTATCGATTCTTCGATTGGAATGTCACCTATGGCGATATTTACCCGCTCGCCATTCGTCAACAGGGGATTTTGATAAATGGGCAATTTCCGGGACCGGATATCTATGCGGTTACGAACGATAATCTTTACATCAATGTTCATAATAGTCTTCCGGAGCCATTTCTTCTGTCTTGGAACGGAATTCAACAGAGGAAAAACTCGTACCAAGACGGAGTGTACGGAACAACGTGCCCAATTCCGCCGGGCAAGAACTTCACCTACAGAATTCAGGTGAAGGATCAGATCGGAAGTTACTTCTATTTCCCCTCTCTTACATTCCACAAGGCCGCCGGTGGATTCGGGAGTATCAGAGTCCTCAGCCGTCCCCAAATTCCGGTCCCATTCCCGGAGCCCGCCGCCGATTACACCCTTCTCGTCGGTGACTGGTACACCGCCAATCACACGACATTGAAGACCATCTTGGATCGTGGTCACAGACTCCCTTTTCCCAATGGAATTGTTATCAATGGCCGTGGTCCGAACGCTACCACTTTCACCGTCGAAAAGGGTAAGACTTACAGGTTTAGAATCTCGAATGTGGGGCTCCAGCATTCGCTCAATTTCAGGGTTCAAGATCATGAGATGAAGCTTGTTGAAGTTGAAGGCACTCACACAATGCAGAATACTTATTCGTCGCTTGATGTTCATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATGCAATCTTGAGCAAGTCTAGATCAAATCCCCTCTCCCGAGCTAGATTCGAAACTTAA

Coding sequence (CDS)

ATGGCATTAAGATCATCACCTAAGTTTTGGTGTTTTCATGTTGCTCTGCTTTTGGGAATGCTGGCCATTGCCGTTGCCGAAAACCCGTATCGATTCTTCGATTGGAATGTCACCTATGGCGATATTTACCCGCTCGCCATTCGTCAACAGGGGATTTTGATAAATGGGCAATTTCCGGGACCGGATATCTATGCGGTTACGAACGATAATCTTTACATCAATGTTCATAATAGTCTTCCGGAGCCATTTCTTCTGTCTTGGAACGGAATTCAACAGAGGAAAAACTCGTACCAAGACGGAGTGTACGGAACAACGTGCCCAATTCCGCCGGGCAAGAACTTCACCTACAGAATTCAGGTGAAGGATCAGATCGGAAGTTACTTCTATTTCCCCTCTCTTACATTCCACAAGGCCGCCGGTGGATTCGGGAGTATCAGAGTCCTCAGCCGTCCCCAAATTCCGGTCCCATTCCCGGAGCCCGCCGCCGATTACACCCTTCTCGTCGGTGACTGGTACACCGCCAATCACACGACATTGAAGACCATCTTGGATCGTGGTCACAGACTCCCTTTTCCCAATGGAATTGTTATCAATGGCCGTGGTCCGAACGCTACCACTTTCACCGTCGAAAAGGGTAAGACTTACAGGTTTAGAATCTCGAATGTGGGGCTCCAGCATTCGCTCAATTTCAGGGTTCAAGATCATGAGATGAAGCTTGTTGAAGTTGAAGGCACTCACACAATGCAGAATACTTATTCGTCGCTTGATGTTCATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATGCAATCTTGAGCAAGTCTAGATCAAATCCCCTCTCCCGAGCTAGATTCGAAACTTAA

Protein sequence

MALRSSPKFWCFHVALLLGMLAIAVAENPYRFFDWNVTYGDIYPLAIRQQGILINGQFPGPDIYAVTNDNLYINVHNSLPEPFLLSWNGIQQRKNSYQDGVYGTTCPIPPGKNFTYRIQVKDQIGSYFYFPSLTFHKAAGGFGSIRVLSRPQIPVPFPEPAADYTLLVGDWYTANHTTLKTILDRGHRLPFPNGIVINGRGPNATTFTVEKGKTYRFRISNVGLQHSLNFRVQDHEMKLVEVEGTHTMQNTYSSLDVHXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAILSKSRSNPLSRARFET
Homology
BLAST of Lag0001315 vs. NCBI nr
Match: XP_008447902.1 (PREDICTED: L-ascorbate oxidase homolog [Cucumis melo])

HSP 1 Score: 501.9 bits (1291), Expect = 3.9e-138
Identity = 234/260 (90.00%), Postives = 248/260 (95.38%), Query Frame = 0

Query: 1   MALRSSPKFW-CFHVALLLGM-LAIAVAENPYRFFDWNVTYGDIYPLAIRQQGILINGQF 60
           MA RSS   W  F+VAL+LG+ + IA A++PYRFFDWNVTYGDIYPL +RQQGILINGQF
Sbjct: 1   MAQRSSTNLWGGFNVALVLGLFINIAFADSPYRFFDWNVTYGDIYPLGVRQQGILINGQF 60

Query: 61  PGPDIYAVTNDNLYINVHNSLPEPFLLSWNGIQQRKNSYQDGVYGTTCPIPPGKNFTYRI 120
           PGPDIYAVTNDN+YINVHNSLPEPFLLSWNG+QQRKNSYQDGVYGTTCPIPPG+NFTYRI
Sbjct: 61  PGPDIYAVTNDNIYINVHNSLPEPFLLSWNGVQQRKNSYQDGVYGTTCPIPPGQNFTYRI 120

Query: 121 QVKDQIGSYFYFPSLTFHKAAGGFGSIRVLSRPQIPVPFPEPAADYTLLVGDWYTANHTT 180
           QVKDQIGSYFYFPSL FHKAAGGFGSIRVLSRP IPVPFPEPAADYTLL+GDWYTANHTT
Sbjct: 121 QVKDQIGSYFYFPSLAFHKAAGGFGSIRVLSRPLIPVPFPEPAADYTLLIGDWYTANHTT 180

Query: 181 LKTILDRGHRLPFPNGIVINGRGPNATTFTVEKGKTYRFRISNVGLQHSLNFRVQDHEMK 240
           LKTILDRGHRLPFPNGIVINGRGP+ATTFTVEKGKTYRFRI+NVGLQHSLNFRVQDHE+K
Sbjct: 181 LKTILDRGHRLPFPNGIVINGRGPSATTFTVEKGKTYRFRITNVGLQHSLNFRVQDHELK 240

Query: 241 LVEVEGTHTMQNTYSSLDVH 259
           LVEVEGTHTMQNTYSSLDVH
Sbjct: 241 LVEVEGTHTMQNTYSSLDVH 260

BLAST of Lag0001315 vs. NCBI nr
Match: XP_022969054.1 (L-ascorbate oxidase homolog [Cucurbita maxima])

HSP 1 Score: 497.7 bits (1280), Expect = 7.4e-137
Identity = 226/251 (90.04%), Postives = 239/251 (95.22%), Query Frame = 0

Query: 8   KFWCFHVALLLGMLAIAVAENPYRFFDWNVTYGDIYPLAIRQQGILINGQFPGPDIYAVT 67
           + WCF+VAL LG + IA+A++PYRFFDWNVTYGDIYPL +RQQGILINGQFPGP+IYAVT
Sbjct: 7   ELWCFYVALALGFMGIAIADSPYRFFDWNVTYGDIYPLGVRQQGILINGQFPGPEIYAVT 66

Query: 68  NDNLYINVHNSLPEPFLLSWNGIQQRKNSYQDGVYGTTCPIPPGKNFTYRIQVKDQIGSY 127
           NDNLYINVHNSLPEPFLLSWNG+QQRKNSYQDGVYGTTCPIPPGKNFTYRIQVKDQIG+Y
Sbjct: 67  NDNLYINVHNSLPEPFLLSWNGVQQRKNSYQDGVYGTTCPIPPGKNFTYRIQVKDQIGTY 126

Query: 128 FYFPSLTFHKAAGGFGSIRVLSRPQIPVPFPEPAADYTLLVGDWYTANHTTLKTILDRGH 187
           FYFPSL FHKAAGGFGSIRVLSRP IPVPFPEPAADYTLL+GDWYTANH+ LKTILD GH
Sbjct: 127 FYFPSLAFHKAAGGFGSIRVLSRPLIPVPFPEPAADYTLLIGDWYTANHSILKTILDGGH 186

Query: 188 RLPFPNGIVINGRGPNATTFTVEKGKTYRFRISNVGLQHSLNFRVQDHEMKLVEVEGTHT 247
           RLP PNGIVINGRGP ATTFTVEKGKTYRFRI+NVGLQHSLNFRVQDHEM LVEVEGTHT
Sbjct: 187 RLPNPNGIVINGRGPQATTFTVEKGKTYRFRITNVGLQHSLNFRVQDHEMTLVEVEGTHT 246

Query: 248 MQNTYSSLDVH 259
           MQNTYSSLDVH
Sbjct: 247 MQNTYSSLDVH 257

BLAST of Lag0001315 vs. NCBI nr
Match: XP_038888634.1 (L-ascorbate oxidase homolog [Benincasa hispida])

HSP 1 Score: 497.7 bits (1280), Expect = 7.4e-137
Identity = 232/259 (89.58%), Postives = 245/259 (94.59%), Query Frame = 0

Query: 1   MALRSSPKFWCFHVALLLGMLA-IAVAENPYRFFDWNVTYGDIYPLAIRQQGILINGQFP 60
           MA RS   +  F+VAL+LG+   +A A++PYRFFDWNVTYGDIYPL IRQ+GILINGQFP
Sbjct: 1   MAQRSPNLWGLFNVALVLGLFVNLAFADSPYRFFDWNVTYGDIYPLGIRQRGILINGQFP 60

Query: 61  GPDIYAVTNDNLYINVHNSLPEPFLLSWNGIQQRKNSYQDGVYGTTCPIPPGKNFTYRIQ 120
           GPDIYAVTNDNLYINVHNSLPEPFLLSWNG+QQRKNSYQDGVYGTTCPIPPG+NFTYRIQ
Sbjct: 61  GPDIYAVTNDNLYINVHNSLPEPFLLSWNGVQQRKNSYQDGVYGTTCPIPPGQNFTYRIQ 120

Query: 121 VKDQIGSYFYFPSLTFHKAAGGFGSIRVLSRPQIPVPFPEPAADYTLLVGDWYTANHTTL 180
           VKDQIGS+FYFPSL FHKAAGGFGSIRVLSRP IPVPFPEPAADYTLL+GDWYTANHTTL
Sbjct: 121 VKDQIGSFFYFPSLAFHKAAGGFGSIRVLSRPLIPVPFPEPAADYTLLIGDWYTANHTTL 180

Query: 181 KTILDRGHRLPFPNGIVINGRGPNATTFTVEKGKTYRFRISNVGLQHSLNFRVQDHEMKL 240
           KTILDRGHRLP PNGIVINGRGPNATTFTVEKGKTYRFRI+NVGLQHSLNFRVQDHEMKL
Sbjct: 181 KTILDRGHRLPLPNGIVINGRGPNATTFTVEKGKTYRFRITNVGLQHSLNFRVQDHEMKL 240

Query: 241 VEVEGTHTMQNTYSSLDVH 259
           VEVEGTHTMQNTYSSLDVH
Sbjct: 241 VEVEGTHTMQNTYSSLDVH 259

BLAST of Lag0001315 vs. NCBI nr
Match: XP_004144874.1 (L-ascorbate oxidase homolog [Cucumis sativus] >KAE8645680.1 hypothetical protein Csa_020201 [Cucumis sativus])

HSP 1 Score: 494.6 bits (1272), Expect = 6.3e-136
Identity = 231/260 (88.85%), Postives = 242/260 (93.08%), Query Frame = 0

Query: 1   MALRSSPKFW-CFHVALLL-GMLAIAVAENPYRFFDWNVTYGDIYPLAIRQQGILINGQF 60
           MA  SS   W  F VAL+    + IA A++PYRFFDWNVTYGDIYPL +RQQGILINGQF
Sbjct: 1   MAQTSSTNLWPAFTVALVFSSFINIAFADSPYRFFDWNVTYGDIYPLGVRQQGILINGQF 60

Query: 61  PGPDIYAVTNDNLYINVHNSLPEPFLLSWNGIQQRKNSYQDGVYGTTCPIPPGKNFTYRI 120
           PGPDIYAVTNDN+YINVHNSLPEPFLLSWNG+QQRKNSYQDGVYGTTCPIPPG+NFTYRI
Sbjct: 61  PGPDIYAVTNDNIYINVHNSLPEPFLLSWNGVQQRKNSYQDGVYGTTCPIPPGQNFTYRI 120

Query: 121 QVKDQIGSYFYFPSLTFHKAAGGFGSIRVLSRPQIPVPFPEPAADYTLLVGDWYTANHTT 180
           QVKDQIGSYFYFPSL FHKAAGGFGSIRVLSRP IPVPFPEPAADYTLL+GDWYTANHTT
Sbjct: 121 QVKDQIGSYFYFPSLAFHKAAGGFGSIRVLSRPLIPVPFPEPAADYTLLIGDWYTANHTT 180

Query: 181 LKTILDRGHRLPFPNGIVINGRGPNATTFTVEKGKTYRFRISNVGLQHSLNFRVQDHEMK 240
           LKTILDRGH+LPFPNGIVINGRGPNATTFTVEKGKTYR RI+NVGLQHSLNFRVQDHEMK
Sbjct: 181 LKTILDRGHKLPFPNGIVINGRGPNATTFTVEKGKTYRLRITNVGLQHSLNFRVQDHEMK 240

Query: 241 LVEVEGTHTMQNTYSSLDVH 259
           LVEVEGTHTMQNTYSSLDVH
Sbjct: 241 LVEVEGTHTMQNTYSSLDVH 260

BLAST of Lag0001315 vs. NCBI nr
Match: XP_022952372.1 (L-ascorbate oxidase homolog [Cucurbita moschata] >KAG6572144.1 hypothetical protein SDJN03_28872, partial [Cucurbita argyrosperma subsp. sororia] >KAG7011793.1 hypothetical protein SDJN02_26699 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 493.4 bits (1269), Expect = 1.4e-135
Identity = 224/251 (89.24%), Postives = 238/251 (94.82%), Query Frame = 0

Query: 8   KFWCFHVALLLGMLAIAVAENPYRFFDWNVTYGDIYPLAIRQQGILINGQFPGPDIYAVT 67
           + WCF+VA+ LG + IA+A++PYRFFDWNVTYGDIYPL +RQQGILINGQFPGP+IYAVT
Sbjct: 7   QLWCFYVAMALGFIGIAIADSPYRFFDWNVTYGDIYPLGVRQQGILINGQFPGPEIYAVT 66

Query: 68  NDNLYINVHNSLPEPFLLSWNGIQQRKNSYQDGVYGTTCPIPPGKNFTYRIQVKDQIGSY 127
           NDNLYINVHNSLPEPFLLSWNG+QQRKNSYQDGVYGTTCPIPPGKNFTYRIQVKDQIG+Y
Sbjct: 67  NDNLYINVHNSLPEPFLLSWNGVQQRKNSYQDGVYGTTCPIPPGKNFTYRIQVKDQIGTY 126

Query: 128 FYFPSLTFHKAAGGFGSIRVLSRPQIPVPFPEPAADYTLLVGDWYTANHTTLKTILDRGH 187
           FYFPSL FHKAAGGFGSIRVLSRP IPVPFPEPAADYTLL+GDWYTANH+ LK ILD GH
Sbjct: 127 FYFPSLAFHKAAGGFGSIRVLSRPLIPVPFPEPAADYTLLIGDWYTANHSILKIILDGGH 186

Query: 188 RLPFPNGIVINGRGPNATTFTVEKGKTYRFRISNVGLQHSLNFRVQDHEMKLVEVEGTHT 247
           RLP PNGIVINGRGP ATTFTVEKGKTYRFRI+NVGLQHSLNFRVQDHEM LVEVEGTHT
Sbjct: 187 RLPNPNGIVINGRGPLATTFTVEKGKTYRFRITNVGLQHSLNFRVQDHEMTLVEVEGTHT 246

Query: 248 MQNTYSSLDVH 259
           MQNTYSSLDVH
Sbjct: 247 MQNTYSSLDVH 257

BLAST of Lag0001315 vs. ExPASy Swiss-Prot
Match: P29162 (L-ascorbate oxidase homolog OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 320.5 bits (820), Expect = 2.1e-86
Identity = 146/250 (58.40%), Postives = 182/250 (72.80%), Query Frame = 0

Query: 14  VALLLGMLAIAVAENPYRFFDWNVTYGDIYPLAIRQQGILINGQFPGPDIYAVTNDNLYI 73
           VALLL +    +AE+PY +F+WNVTYG I PL + QQGILINGQFPGP I   +N+N+ +
Sbjct: 9   VALLLCLSVGVIAEDPYLYFNWNVTYGTIAPLGVPQQGILINGQFPGPRINCTSNNNIVV 68

Query: 74  NVHNSLPEPFLLSWNGIQQRKNSYQDGVYGTTCPIPPGKNFTYRIQVKDQIGSYFYFPSL 133
           NV N+L EPFL +WNG+Q RKNS+QDG  GT CPI PG+NFTYR QVKDQIGSY YFP+ 
Sbjct: 69  NVFNNLDEPFLFTWNGVQHRKNSWQDGTPGTMCPIMPGQNFTYRFQVKDQIGSYSYFPTT 128

Query: 134 TFHKAAGGFGSIRVLSRPQIPVPFPEPAADYTLLVGDWYTANHTTLKTILDRGHRLPFPN 193
             H+AAGG+G++ V SR  IPVPF  PA +Y + VGDWY   H TLK ILD G  +  P+
Sbjct: 129 ALHRAAGGYGALNVHSRALIPVPFDNPADEYNVFVGDWYNKGHKTLKKILDGGRTIGRPD 188

Query: 194 GIVINGRGPNA-----TTFTVEKGKTYRFRISNVGLQHSLNFRVQDHEMKLVEVEGTHTM 253
           GI+ING+           FT+E GKTYR+R  N+G++ S+N R Q H MKLVE+EG+HT+
Sbjct: 189 GIIINGKSAKVGEAKEPLFTMEAGKTYRYRFCNLGMRSSVNIRFQGHPMKLVELEGSHTV 248

Query: 254 QNTYSSLDVH 259
           QN Y SLD+H
Sbjct: 249 QNIYDSLDLH 258

BLAST of Lag0001315 vs. ExPASy Swiss-Prot
Match: Q00624 (L-ascorbate oxidase homolog OS=Brassica napus OX=3708 GN=Bp10 PE=2 SV=1)

HSP 1 Score: 312.4 bits (799), Expect = 5.8e-84
Identity = 143/238 (60.08%), Postives = 179/238 (75.21%), Query Frame = 0

Query: 26  AENPYRFFDWNVTYGDIYPLAIRQQGILINGQFPGPDIYAVTNDNLYINVHNSLPEPFLL 85
           AE+PY    WNVTYG   PL + QQ ILINGQFPGP+I + +N+N+ INV N+L EPFLL
Sbjct: 23  AEDPYFHHVWNVTYGTASPLGVPQQVILINGQFPGPNINSTSNNNVIINVFNNLDEPFLL 82

Query: 86  SWNGIQQRKNSYQDGVYGTTCPIPPGKNFTYRIQVKDQIGSYFYFPSLTFHKAAGGFGSI 145
           +WNGIQ RKN +QDG  GT CPI PG N+TY  Q KDQIGSYFY+P+   H+AAGG+G +
Sbjct: 83  TWNGIQHRKNCWQDGTPGTMCPIMPGTNYTYHFQPKDQIGSYFYYPTTGMHRAAGGYGGL 142

Query: 146 RVLSRPQIPVPFPEPAADYTLLVGDWYTANHTTLKTILDRGHRLPFPNGIVINGR----- 205
           RV SR  IPVP+ +P  DYT+L+GDWYT +HT LK  LD G  +  P+GIVING+     
Sbjct: 143 RVNSRLLIPVPYADPEDDYTVLIGDWYTKSHTQLKKFLDGGRTIGRPDGIVINGKSGKGD 202

Query: 206 GPNATTFTVEKGKTYRFRISNVGLQHSLNFRVQDHEMKLVEVEGTHTMQNTYSSLDVH 259
           G +A  FT++ GKTYR RI NVG++ S+NFR+Q+H+MKLVE+EG+H +QN Y SLDVH
Sbjct: 203 GSDAPLFTLKPGKTYRVRICNVGVKTSINFRIQNHKMKLVEMEGSHVLQNDYDSLDVH 260

BLAST of Lag0001315 vs. ExPASy Swiss-Prot
Match: Q9SU40 (Monocopper oxidase-like protein SKU5 OS=Arabidopsis thaliana OX=3702 GN=SKU5 PE=1 SV=1)

HSP 1 Score: 283.1 bits (723), Expect = 3.8e-75
Identity = 137/261 (52.49%), Postives = 173/261 (66.28%), Query Frame = 0

Query: 12  FHVALLLGMLAIA--VAENPYRFFDWNVTYGDIYPLAIRQQGILINGQFPGPDIYAVTND 71
           F + LL+  + I+   A +PY F+++ V+Y    PL + QQ I ING+FPGP I   TN+
Sbjct: 4   FKILLLVFFVNISFCFAADPYSFYNFEVSYITASPLGVPQQVIAINGKFPGPTINVTTNE 63

Query: 72  NLYINVHNSLPEPFLLSWNGIQQRKNSYQDGVYGTTCPIPPGKNFTYRIQVKDQIGSYFY 131
           NL +NV N L E  LL WNGIQQR+ S+QDGV GT CPIPP  N+TY  QVKDQIGS+FY
Sbjct: 64  NLVVNVRNKLDEGLLLHWNGIQQRRVSWQDGVLGTNCPIPPKWNWTYEFQVKDQIGSFFY 123

Query: 132 FPSLTFHKAAGGFGSIRVLSRPQIPVPFPEPAADYTLLVGDWYTANHTTLKTILDRGHRL 191
           FPSL F +A+GGFGS  V  R  IPVPF  P  D T+ +GDWY  NHT L+  LD G  L
Sbjct: 124 FPSLHFQRASGGFGSFVVNPRAIIPVPFSTPDGDITVTIGDWYIRNHTALRKALDDGKDL 183

Query: 192 PFPNGIVINGRGP------------NATTFTVEKGKTYRFRISNVGLQHSLNFRVQDHEM 251
             P+G++ING+GP            +  T TV  GKTYR R+SNVG+  SLNFR+Q H +
Sbjct: 184 GMPDGVLINGKGPYRYNDTLVADGIDFETITVHPGKTYRLRVSNVGISTSLNFRIQGHNL 243

Query: 252 KLVEVEGTHTMQNTYSSLDVH 259
            L E EG++T+Q  Y+SLD+H
Sbjct: 244 VLAESEGSYTVQQNYTSLDIH 264

BLAST of Lag0001315 vs. ExPASy Swiss-Prot
Match: Q8VXX5 (Monocopper oxidase-like protein SKS1 OS=Arabidopsis thaliana OX=3702 GN=SKS1 PE=2 SV=1)

HSP 1 Score: 277.7 bits (709), Expect = 1.6e-73
Identity = 127/248 (51.21%), Postives = 168/248 (67.74%), Query Frame = 0

Query: 22  AIAVAENPYRFFDWNVTYGDIYPLAIRQQGILINGQFPGPDIYAVTNDNLYINVHNSLPE 81
           A++ A +P+  +D+ V+Y    PL + QQ I +NGQFPGP + A TN N+ +NV N L E
Sbjct: 20  AVSFAADPFVSYDFRVSYLTASPLGVPQQVIAVNGQFPGPLLNATTNYNVVVNVFNHLDE 79

Query: 82  PFLLSWNGIQQRKNSYQDGVYGTTCPIPPGKNFTYRIQVKDQIGSYFYFPSLTFHKAAGG 141
           P LL+W GIQ R+NS+QDGV GT CPIPP  NFTY+ QVKDQIGS+FY PSL F +A+GG
Sbjct: 80  PLLLTWPGIQMRRNSWQDGVLGTNCPIPPRWNFTYQFQVKDQIGSFFYSPSLNFQRASGG 139

Query: 142 FGSIRVLSRPQIPVPFPEPAADYTLLVGDWYTANHTTLKTILDRGHRLPFPNGIVINGRG 201
           FG I + +R  IP+PFP+P  +   ++GDWYT +H  L+  LD G  L  P+G++ING+G
Sbjct: 140 FGPIVINNRDIIPIPFPQPDGELIFIIGDWYTQDHKALRRALDSGKELGMPDGVLINGKG 199

Query: 202 P-----------NATTFTVEKGKTYRFRISNVGLQHSLNFRVQDHEMKLVEVEGTHTMQN 259
           P           +  TF VE GKTYR R+ NVG+  SLNFR+Q+H + LVE EG +T Q 
Sbjct: 200 PYKYNSSVPDGIDYLTFHVEPGKTYRIRVHNVGISTSLNFRIQNHSLLLVETEGHYTSQA 259

BLAST of Lag0001315 vs. ExPASy Swiss-Prot
Match: Q9FHN6 (Monocopper oxidase-like protein SKS2 OS=Arabidopsis thaliana OX=3702 GN=SKS2 PE=2 SV=1)

HSP 1 Score: 277.3 bits (708), Expect = 2.1e-73
Identity = 127/258 (49.22%), Postives = 172/258 (66.67%), Query Frame = 0

Query: 12  FHVALLLGMLAIAVAENPYRFFDWNVTYGDIYPLAIRQQGILINGQFPGPDIYAVTNDNL 71
           F  AL+ G    + A +PY  +D+ ++Y    PL + QQ I +NG+FPGP I A TN N+
Sbjct: 12  FSFALIFG---FSFAGDPYVSYDFTLSYITASPLGVPQQVIAVNGKFPGPVINATTNYNV 71

Query: 72  YINVHNSLPEPFLLSWNGIQQRKNSYQDGVYGTTCPIPPGKNFTYRIQVKDQIGSYFYFP 131
           ++NV N L EP LL+W G+Q R+NS+QDGV GT CPIPP  NFTY  Q+KDQIGSYFY P
Sbjct: 72  HVNVLNHLDEPLLLTWPGVQMRRNSWQDGVLGTNCPIPPNWNFTYDFQLKDQIGSYFYSP 131

Query: 132 SLTFHKAAGGFGSIRVLSRPQIPVPFPEPAADYTLLVGDWYTANHTTLKTILDRGHRLPF 191
           SL F +A+GGFG++ + +R  +P+PF EP  +   ++GDWYT NHT L+ ILD G  L  
Sbjct: 132 SLNFQRASGGFGALIINNRDLVPIPFTEPDGEIIFIIGDWYTQNHTALRRILDSGKELGM 191

Query: 192 PNGIVINGRGP-----------NATTFTVEKGKTYRFRISNVGLQHSLNFRVQDHEMKLV 251
           P+G++ING+GP              T  V+ GKTYR R+ NVG+  SLNFR+Q+H++ L+
Sbjct: 192 PDGVLINGKGPFKYNSSVPDGIEHETVNVDPGKTYRIRVHNVGISTSLNFRIQNHKLLLI 251

Query: 252 EVEGTHTMQNTYSSLDVH 259
           E EG +T Q  ++  DVH
Sbjct: 252 ETEGRYTSQMNFTDFDVH 266

BLAST of Lag0001315 vs. ExPASy TrEMBL
Match: A0A1S3BIH0 (L-ascorbate oxidase homolog OS=Cucumis melo OX=3656 GN=LOC103490248 PE=3 SV=1)

HSP 1 Score: 501.9 bits (1291), Expect = 1.9e-138
Identity = 234/260 (90.00%), Postives = 248/260 (95.38%), Query Frame = 0

Query: 1   MALRSSPKFW-CFHVALLLGM-LAIAVAENPYRFFDWNVTYGDIYPLAIRQQGILINGQF 60
           MA RSS   W  F+VAL+LG+ + IA A++PYRFFDWNVTYGDIYPL +RQQGILINGQF
Sbjct: 1   MAQRSSTNLWGGFNVALVLGLFINIAFADSPYRFFDWNVTYGDIYPLGVRQQGILINGQF 60

Query: 61  PGPDIYAVTNDNLYINVHNSLPEPFLLSWNGIQQRKNSYQDGVYGTTCPIPPGKNFTYRI 120
           PGPDIYAVTNDN+YINVHNSLPEPFLLSWNG+QQRKNSYQDGVYGTTCPIPPG+NFTYRI
Sbjct: 61  PGPDIYAVTNDNIYINVHNSLPEPFLLSWNGVQQRKNSYQDGVYGTTCPIPPGQNFTYRI 120

Query: 121 QVKDQIGSYFYFPSLTFHKAAGGFGSIRVLSRPQIPVPFPEPAADYTLLVGDWYTANHTT 180
           QVKDQIGSYFYFPSL FHKAAGGFGSIRVLSRP IPVPFPEPAADYTLL+GDWYTANHTT
Sbjct: 121 QVKDQIGSYFYFPSLAFHKAAGGFGSIRVLSRPLIPVPFPEPAADYTLLIGDWYTANHTT 180

Query: 181 LKTILDRGHRLPFPNGIVINGRGPNATTFTVEKGKTYRFRISNVGLQHSLNFRVQDHEMK 240
           LKTILDRGHRLPFPNGIVINGRGP+ATTFTVEKGKTYRFRI+NVGLQHSLNFRVQDHE+K
Sbjct: 181 LKTILDRGHRLPFPNGIVINGRGPSATTFTVEKGKTYRFRITNVGLQHSLNFRVQDHELK 240

Query: 241 LVEVEGTHTMQNTYSSLDVH 259
           LVEVEGTHTMQNTYSSLDVH
Sbjct: 241 LVEVEGTHTMQNTYSSLDVH 260

BLAST of Lag0001315 vs. ExPASy TrEMBL
Match: A0A6J1I1G1 (L-ascorbate oxidase homolog OS=Cucurbita maxima OX=3661 GN=LOC111468166 PE=3 SV=1)

HSP 1 Score: 497.7 bits (1280), Expect = 3.6e-137
Identity = 226/251 (90.04%), Postives = 239/251 (95.22%), Query Frame = 0

Query: 8   KFWCFHVALLLGMLAIAVAENPYRFFDWNVTYGDIYPLAIRQQGILINGQFPGPDIYAVT 67
           + WCF+VAL LG + IA+A++PYRFFDWNVTYGDIYPL +RQQGILINGQFPGP+IYAVT
Sbjct: 7   ELWCFYVALALGFMGIAIADSPYRFFDWNVTYGDIYPLGVRQQGILINGQFPGPEIYAVT 66

Query: 68  NDNLYINVHNSLPEPFLLSWNGIQQRKNSYQDGVYGTTCPIPPGKNFTYRIQVKDQIGSY 127
           NDNLYINVHNSLPEPFLLSWNG+QQRKNSYQDGVYGTTCPIPPGKNFTYRIQVKDQIG+Y
Sbjct: 67  NDNLYINVHNSLPEPFLLSWNGVQQRKNSYQDGVYGTTCPIPPGKNFTYRIQVKDQIGTY 126

Query: 128 FYFPSLTFHKAAGGFGSIRVLSRPQIPVPFPEPAADYTLLVGDWYTANHTTLKTILDRGH 187
           FYFPSL FHKAAGGFGSIRVLSRP IPVPFPEPAADYTLL+GDWYTANH+ LKTILD GH
Sbjct: 127 FYFPSLAFHKAAGGFGSIRVLSRPLIPVPFPEPAADYTLLIGDWYTANHSILKTILDGGH 186

Query: 188 RLPFPNGIVINGRGPNATTFTVEKGKTYRFRISNVGLQHSLNFRVQDHEMKLVEVEGTHT 247
           RLP PNGIVINGRGP ATTFTVEKGKTYRFRI+NVGLQHSLNFRVQDHEM LVEVEGTHT
Sbjct: 187 RLPNPNGIVINGRGPQATTFTVEKGKTYRFRITNVGLQHSLNFRVQDHEMTLVEVEGTHT 246

Query: 248 MQNTYSSLDVH 259
           MQNTYSSLDVH
Sbjct: 247 MQNTYSSLDVH 257

BLAST of Lag0001315 vs. ExPASy TrEMBL
Match: A0A6J1GK88 (L-ascorbate oxidase homolog OS=Cucurbita moschata OX=3662 GN=LOC111455076 PE=3 SV=1)

HSP 1 Score: 493.4 bits (1269), Expect = 6.8e-136
Identity = 224/251 (89.24%), Postives = 238/251 (94.82%), Query Frame = 0

Query: 8   KFWCFHVALLLGMLAIAVAENPYRFFDWNVTYGDIYPLAIRQQGILINGQFPGPDIYAVT 67
           + WCF+VA+ LG + IA+A++PYRFFDWNVTYGDIYPL +RQQGILINGQFPGP+IYAVT
Sbjct: 7   QLWCFYVAMALGFIGIAIADSPYRFFDWNVTYGDIYPLGVRQQGILINGQFPGPEIYAVT 66

Query: 68  NDNLYINVHNSLPEPFLLSWNGIQQRKNSYQDGVYGTTCPIPPGKNFTYRIQVKDQIGSY 127
           NDNLYINVHNSLPEPFLLSWNG+QQRKNSYQDGVYGTTCPIPPGKNFTYRIQVKDQIG+Y
Sbjct: 67  NDNLYINVHNSLPEPFLLSWNGVQQRKNSYQDGVYGTTCPIPPGKNFTYRIQVKDQIGTY 126

Query: 128 FYFPSLTFHKAAGGFGSIRVLSRPQIPVPFPEPAADYTLLVGDWYTANHTTLKTILDRGH 187
           FYFPSL FHKAAGGFGSIRVLSRP IPVPFPEPAADYTLL+GDWYTANH+ LK ILD GH
Sbjct: 127 FYFPSLAFHKAAGGFGSIRVLSRPLIPVPFPEPAADYTLLIGDWYTANHSILKIILDGGH 186

Query: 188 RLPFPNGIVINGRGPNATTFTVEKGKTYRFRISNVGLQHSLNFRVQDHEMKLVEVEGTHT 247
           RLP PNGIVINGRGP ATTFTVEKGKTYRFRI+NVGLQHSLNFRVQDHEM LVEVEGTHT
Sbjct: 187 RLPNPNGIVINGRGPLATTFTVEKGKTYRFRITNVGLQHSLNFRVQDHEMTLVEVEGTHT 246

Query: 248 MQNTYSSLDVH 259
           MQNTYSSLDVH
Sbjct: 247 MQNTYSSLDVH 257

BLAST of Lag0001315 vs. ExPASy TrEMBL
Match: A0A6J1C5Y9 (L-ascorbate oxidase homolog OS=Momordica charantia OX=3673 GN=LOC111007682 PE=3 SV=1)

HSP 1 Score: 492.3 bits (1266), Expect = 1.5e-135
Identity = 223/253 (88.14%), Postives = 238/253 (94.07%), Query Frame = 0

Query: 6   SPKFWCFHVALLLGMLAIAVAENPYRFFDWNVTYGDIYPLAIRQQGILINGQFPGPDIYA 65
           +PK WCF V  +  M+ IA +++PYRFFDWNVTYGDIYPL +RQQGILINGQFPGPDIYA
Sbjct: 9   TPKLWCFIVFWMSVMINIAFSDSPYRFFDWNVTYGDIYPLGVRQQGILINGQFPGPDIYA 68

Query: 66  VTNDNLYINVHNSLPEPFLLSWNGIQQRKNSYQDGVYGTTCPIPPGKNFTYRIQVKDQIG 125
           VTNDNLYINVHNSLPEPFLLSW+G+QQRKNSYQDGVYGTTCPIPPGKNFTYRIQVKDQIG
Sbjct: 69  VTNDNLYINVHNSLPEPFLLSWSGVQQRKNSYQDGVYGTTCPIPPGKNFTYRIQVKDQIG 128

Query: 126 SYFYFPSLTFHKAAGGFGSIRVLSRPQIPVPFPEPAADYTLLVGDWYTANHTTLKTILDR 185
           S+FYFPSL  HKAAGGFGSIRVLSRP IPVPFP+PA D+TLL+GDWYT NH TL+TILDR
Sbjct: 129 SFFYFPSLALHKAAGGFGSIRVLSRPLIPVPFPDPAGDFTLLIGDWYTTNHKTLQTILDR 188

Query: 186 GHRLPFPNGIVINGRGPNATTFTVEKGKTYRFRISNVGLQHSLNFRVQDHEMKLVEVEGT 245
           GHRLPFPNGIVINGRGPNATTFTVEKGKTYRFRISNVGL+HSLNFRVQDHEMKLVEVEGT
Sbjct: 189 GHRLPFPNGIVINGRGPNATTFTVEKGKTYRFRISNVGLEHSLNFRVQDHEMKLVEVEGT 248

Query: 246 HTMQNTYSSLDVH 259
           HTMQNTYSSLDVH
Sbjct: 249 HTMQNTYSSLDVH 261

BLAST of Lag0001315 vs. ExPASy TrEMBL
Match: A0A2I4EWY0 (L-ascorbate oxidase homolog OS=Juglans regia OX=51240 GN=LOC108993432 PE=3 SV=1)

HSP 1 Score: 453.4 bits (1165), Expect = 7.8e-124
Identity = 202/258 (78.29%), Postives = 231/258 (89.53%), Query Frame = 0

Query: 1   MALRSSPKFWCFHVALLLGMLAIAVAENPYRFFDWNVTYGDIYPLAIRQQGILINGQFPG 60
           M L+ +  F     A+ L +++I  AE+PYRFF+WNVTYGDIYPL +RQQGILINGQFPG
Sbjct: 1   MLLKQAGLFAWRLCAIFLALISIVTAEDPYRFFNWNVTYGDIYPLGVRQQGILINGQFPG 60

Query: 61  PDIYAVTNDNLYINVHNSLPEPFLLSWNGIQQRKNSYQDGVYGTTCPIPPGKNFTYRIQV 120
           P+IY+VTNDNL INVHNSLPEPFLLSWNG+QQRKNSYQDGVYGTTCPIPPGKNFTY +Q+
Sbjct: 61  PEIYSVTNDNLIINVHNSLPEPFLLSWNGVQQRKNSYQDGVYGTTCPIPPGKNFTYTLQM 120

Query: 121 KDQIGSYFYFPSLTFHKAAGGFGSIRVLSRPQIPVPFPEPAADYTLLVGDWYTANHTTLK 180
           KDQIGS+FYFPSL FHKAAGGFG+I+VLSRP+IPVPFPEPA DY+LL+GDWY  NHT LK
Sbjct: 121 KDQIGSFFYFPSLAFHKAAGGFGAIKVLSRPRIPVPFPEPAGDYSLLIGDWYKTNHTKLK 180

Query: 181 TILDRGHRLPFPNGIVINGRGPNATTFTVEKGKTYRFRISNVGLQHSLNFRVQDHEMKLV 240
            ILDRGHRLPFP+GI+ING+GPN T+FTVE+GKTYR RISNVGLQHSLNFR+Q H+MKLV
Sbjct: 181 AILDRGHRLPFPDGILINGQGPNGTSFTVEQGKTYRLRISNVGLQHSLNFRIQGHKMKLV 240

Query: 241 EVEGTHTMQNTYSSLDVH 259
           EVEGTHT+Q TYS LD+H
Sbjct: 241 EVEGTHTVQTTYSELDIH 258

BLAST of Lag0001315 vs. TAIR 10
Match: AT1G76160.1 (SKU5 similar 5 )

HSP 1 Score: 404.4 bits (1038), Expect = 8.0e-113
Identity = 184/249 (73.90%), Postives = 215/249 (86.35%), Query Frame = 0

Query: 12  FHVALLLG--MLAIAVAENPYRFFDWNVTYGDIYPLAIRQQGILINGQFPGPDIYAVTND 71
           F  AL +G  +L    AE+PYRFF+WN+TYGDIYPL +RQQGILING FPGPDI++VTND
Sbjct: 7   FAAALFIGLSLLFAVTAEDPYRFFEWNITYGDIYPLGVRQQGILINGAFPGPDIHSVTND 66

Query: 72  NLYINVHNSLPEPFLLSWNGIQQRKNSYQDGVYGTTCPIPPGKNFTYRIQVKDQIGSYFY 131
           NL INV+NSL EPFLLSWNGIQQR+NS+ DGVYGTTCPIPPGKN+TY +Q+KDQIGS++Y
Sbjct: 67  NLIINVYNSLDEPFLLSWNGIQQRRNSFVDGVYGTTCPIPPGKNYTYILQMKDQIGSFYY 126

Query: 132 FPSLTFHKAAGGFGSIRVLSRPQIPVPFPEPAADYTLLVGDWYTANHTTLKTILDRGHRL 191
           FPSL FHKAAGGFG IR+LSRP+IPVPFP+PA D T+L+GDWY ANHT L+  LD G +L
Sbjct: 127 FPSLGFHKAAGGFGGIRILSRPRIPVPFPDPAGDTTVLIGDWYKANHTDLRAQLDNGKKL 186

Query: 192 PFPNGIVINGRGPNATTFTVEKGKTYRFRISNVGLQHSLNFRVQDHEMKLVEVEGTHTMQ 251
           P P+GI+INGR   A T  VE+GKTYRFRISNVGLQ SLNFR+QDH+MK+VEVEGTHT+Q
Sbjct: 187 PLPDGILINGRSSGA-TLNVEQGKTYRFRISNVGLQDSLNFRIQDHKMKVVEVEGTHTLQ 246

Query: 252 NTYSSLDVH 259
            T+SSLDVH
Sbjct: 247 TTFSSLDVH 254

BLAST of Lag0001315 vs. TAIR 10
Match: AT1G41830.1 (SKU5-similar 6 )

HSP 1 Score: 399.8 bits (1026), Expect = 2.0e-111
Identity = 180/241 (74.69%), Postives = 205/241 (85.06%), Query Frame = 0

Query: 18  LGMLAIAVAENPYRFFDWNVTYGDIYPLAIRQQGILINGQFPGPDIYAVTNDNLYINVHN 77
           L   A   AE+PYRFFDWNVTYGDIYPL +RQQGILINGQFPGPDI++VTNDNL INVHN
Sbjct: 16  LSFFAAVTAESPYRFFDWNVTYGDIYPLGVRQQGILINGQFPGPDIHSVTNDNLIINVHN 75

Query: 78  SLPEPFLLSWNGIQQRKNSYQDGVYGTTCPIPPGKNFTYRIQVKDQIGSYFYFPSLTFHK 137
           SL EPFL+SWNG+Q R+NSY DG+YGTTCPIPP  N+TY +QVKDQIGS++YFPSL FHK
Sbjct: 76  SLDEPFLISWNGVQNRRNSYVDGMYGTTCPIPPRSNYTYILQVKDQIGSFYYFPSLAFHK 135

Query: 138 AAGGFGSIRVLSRPQIPVPFPEPAADYTLLVGDWYTANHTTLKTILDRGHRLPFPNGIVI 197
           AAGGFG IR+LSRP IPVPF +PA DYT+L+GDWY  NHT LK+ LDRG +LP P+GI+I
Sbjct: 136 AAGGFGGIRILSRPGIPVPFADPAGDYTVLIGDWYKFNHTDLKSRLDRGRKLPSPDGILI 195

Query: 198 NGRGPNATTFTVEKGKTYRFRISNVGLQHSLNFRVQDHEMKLVEVEGTHTMQNTYSSLDV 257
           NGR  N  T  VE+GKTYR RISNVGLQ SLNFR+Q+H MKLVEVEGTHT+Q  +SSLDV
Sbjct: 196 NGRS-NGATLNVEQGKTYRLRISNVGLQDSLNFRIQNHRMKLVEVEGTHTLQTMFSSLDV 255

Query: 258 H 259
           H
Sbjct: 256 H 255

BLAST of Lag0001315 vs. TAIR 10
Match: AT4G38420.1 (SKU5 similar 9 )

HSP 1 Score: 399.8 bits (1026), Expect = 2.0e-111
Identity = 176/244 (72.13%), Postives = 210/244 (86.07%), Query Frame = 0

Query: 16  LLLGMLAIAVAENPYRFFDWNVTYGDIYPLAIRQQGILINGQFPGPDIYAVTNDNLYINV 75
           + + +++   A++PYRFFDW VTYG+I PL I Q+GILINGQ+PGPDIY+VTNDNL INV
Sbjct: 15  MTISIISFVQADDPYRFFDWRVTYGNISPLGIPQRGILINGQYPGPDIYSVTNDNLIINV 74

Query: 76  HNSLPEPFLLSWNGIQQRKNSYQDGVYGTTCPIPPGKNFTYRIQVKDQIGSYFYFPSLTF 135
           HN L EPFLLSWNG+Q RKNSYQDGVYGTTCPIPPGKN+TY IQVKDQIGS+FYFPSL  
Sbjct: 75  HNDLDEPFLLSWNGVQLRKNSYQDGVYGTTCPIPPGKNYTYAIQVKDQIGSFFYFPSLAV 134

Query: 136 HKAAGGFGSIRVLSRPQIPVPFPEPAADYTLLVGDWYTANHTTLKTILDRGHRLPFPNGI 195
           HKAAGGFG  R+LSRP+IPVPFPEPA D+T L+GDW+  +H  LK ILDRGH+LP P G+
Sbjct: 135 HKAAGGFGGFRILSRPRIPVPFPEPAGDFTFLIGDWFKHDHKVLKAILDRGHKLPLPQGV 194

Query: 196 VINGRGPN-ATTFTVEKGKTYRFRISNVGLQHSLNFRVQDHEMKLVEVEGTHTMQNTYSS 255
           +ING+G +  ++ TV KGKTYRFRISNVGLQH+LNFR+Q H+MKLVEVEGTHT+Q+ Y+S
Sbjct: 195 LINGQGVSYMSSITVHKGKTYRFRISNVGLQHTLNFRIQGHQMKLVEVEGTHTVQSMYTS 254

Query: 256 LDVH 259
           LD+H
Sbjct: 255 LDIH 258

BLAST of Lag0001315 vs. TAIR 10
Match: AT1G21850.1 (SKU5 similar 8 )

HSP 1 Score: 382.1 bits (980), Expect = 4.2e-106
Identity = 173/247 (70.04%), Postives = 209/247 (84.62%), Query Frame = 0

Query: 15  ALLLG---MLAIAVAENPYRFFDWNVTYGDIYPLAIRQQGILINGQFPGPDIYAVTNDNL 74
           A++LG   +++   AE+PY+FF+W+VTYG+I PL + QQGILING+FPGPDI AVTNDNL
Sbjct: 10  AMILGLFFLISFVAAEDPYKFFEWHVTYGNISPLKVAQQGILINGKFPGPDIAAVTNDNL 69

Query: 75  YINVHNSLPEPFLLSWNGIQQRKNSYQDGVYGTTCPIPPGKNFTYRIQVKDQIGSYFYFP 134
            INV N L EPFL+SW+GI+  +NSYQDGVYGTTCPIPPGKN+TY +QVKDQIGS++YFP
Sbjct: 70  IINVFNHLDEPFLISWSGIRNWRNSYQDGVYGTTCPIPPGKNYTYALQVKDQIGSFYYFP 129

Query: 135 SLTFHKAAGGFGSIRVLSRPQIPVPFPEPAADYTLLVGDWYTANHTTLKTILDRGHRLPF 194
           SL FHKAAGGFG+IR+ SRP+IPVPFP PA DYT+L+GDWY  NH  L+  LD G +LPF
Sbjct: 130 SLGFHKAAGGFGAIRISSRPRIPVPFPAPAGDYTVLIGDWYKTNHKDLRAQLDNGGKLPF 189

Query: 195 PNGIVINGRGPNATTFTVEKGKTYRFRISNVGLQHSLNFRVQDHEMKLVEVEGTHTMQNT 254
           P+GI+INGRG  A T  +E GKTYR RISNVGLQ+SLNFR+Q+H+MKLVEVEGTHT+Q  
Sbjct: 190 PDGILINGRGSGA-TLNIEPGKTYRLRISNVGLQNSLNFRIQNHKMKLVEVEGTHTIQTP 249

Query: 255 YSSLDVH 259
           +SSLDVH
Sbjct: 250 FSSLDVH 255

BLAST of Lag0001315 vs. TAIR 10
Match: AT4G22010.1 (SKU5 similar 4 )

HSP 1 Score: 375.9 bits (964), Expect = 3.0e-104
Identity = 168/245 (68.57%), Postives = 200/245 (81.63%), Query Frame = 0

Query: 14  VALLLGMLAIAVAENPYRFFDWNVTYGDIYPLAIRQQGILINGQFPGPDIYAVTNDNLYI 73
           + LLL ++   + +NPYRFF W +TYGDIYPL ++QQGILINGQFPGP I A+TNDN+ I
Sbjct: 9   IVLLLVLINGVLGDNPYRFFTWKITYGDIYPLGVKQQGILINGQFPGPHIDAITNDNIII 68

Query: 74  NVHNSLPEPFLLSWNGIQQRKNSYQDGVYGTTCPIPPGKNFTYRIQVKDQIGSYFYFPSL 133
           +V N L EPFL+SWNG+QQRKNS+QDGV GTTCPIPPGKNFTY IQVKDQIGS++YFPSL
Sbjct: 69  SVFNYLKEPFLISWNGVQQRKNSWQDGVVGTTCPIPPGKNFTYVIQVKDQIGSFYYFPSL 128

Query: 134 TFHKAAGGFGSIRVLSRPQIPVPFPEPAADYTLLVGDWYTANHTTLKTILDRGHRLPFPN 193
            FHKAAG FG+IRV SRP+IPVPF  P  D+ LL GDWY  NH  L+ +L+ G  LP P+
Sbjct: 129 AFHKAAGAFGAIRVWSRPRIPVPFSPPDGDFWLLAGDWYKTNHYVLRRLLEAGRNLPNPD 188

Query: 194 GIVINGRGPNATTFTVEKGKTYRFRISNVGLQHSLNFRVQDHEMKLVEVEGTHTMQNTYS 253
           G++INGRG    TFTV+ GKTYRFRISNVG+  SLNFR+Q H MKLVEVEG+HT+QN Y+
Sbjct: 189 GVLINGRGWGGNTFTVQPGKTYRFRISNVGVATSLNFRIQGHTMKLVEVEGSHTVQNIYT 248

Query: 254 SLDVH 259
           SLD+H
Sbjct: 249 SLDIH 253

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008447902.13.9e-13890.00PREDICTED: L-ascorbate oxidase homolog [Cucumis melo][more]
XP_022969054.17.4e-13790.04L-ascorbate oxidase homolog [Cucurbita maxima][more]
XP_038888634.17.4e-13789.58L-ascorbate oxidase homolog [Benincasa hispida][more]
XP_004144874.16.3e-13688.85L-ascorbate oxidase homolog [Cucumis sativus] >KAE8645680.1 hypothetical protein... [more]
XP_022952372.11.4e-13589.24L-ascorbate oxidase homolog [Cucurbita moschata] >KAG6572144.1 hypothetical prot... [more]
Match NameE-valueIdentityDescription
P291622.1e-8658.40L-ascorbate oxidase homolog OS=Nicotiana tabacum OX=4097 PE=2 SV=1[more]
Q006245.8e-8460.08L-ascorbate oxidase homolog OS=Brassica napus OX=3708 GN=Bp10 PE=2 SV=1[more]
Q9SU403.8e-7552.49Monocopper oxidase-like protein SKU5 OS=Arabidopsis thaliana OX=3702 GN=SKU5 PE=... [more]
Q8VXX51.6e-7351.21Monocopper oxidase-like protein SKS1 OS=Arabidopsis thaliana OX=3702 GN=SKS1 PE=... [more]
Q9FHN62.1e-7349.22Monocopper oxidase-like protein SKS2 OS=Arabidopsis thaliana OX=3702 GN=SKS2 PE=... [more]
Match NameE-valueIdentityDescription
A0A1S3BIH01.9e-13890.00L-ascorbate oxidase homolog OS=Cucumis melo OX=3656 GN=LOC103490248 PE=3 SV=1[more]
A0A6J1I1G13.6e-13790.04L-ascorbate oxidase homolog OS=Cucurbita maxima OX=3661 GN=LOC111468166 PE=3 SV=... [more]
A0A6J1GK886.8e-13689.24L-ascorbate oxidase homolog OS=Cucurbita moschata OX=3662 GN=LOC111455076 PE=3 S... [more]
A0A6J1C5Y91.5e-13588.14L-ascorbate oxidase homolog OS=Momordica charantia OX=3673 GN=LOC111007682 PE=3 ... [more]
A0A2I4EWY07.8e-12478.29L-ascorbate oxidase homolog OS=Juglans regia OX=51240 GN=LOC108993432 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G76160.18.0e-11373.90SKU5 similar 5 [more]
AT1G41830.12.0e-11174.69SKU5-similar 6 [more]
AT4G38420.12.0e-11172.13SKU5 similar 9 [more]
AT1G21850.14.2e-10670.04SKU5 similar 8 [more]
AT4G22010.13.0e-10468.57SKU5 similar 4 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001117Multicopper oxidase, type 1PFAMPF00394Cu-oxidasecoord: 163..259
e-value: 4.3E-26
score: 91.9
IPR008972CupredoxinGENE3D2.60.40.420coord: 30..152
e-value: 2.1E-31
score: 110.7
IPR008972CupredoxinGENE3D2.60.40.420coord: 160..259
e-value: 2.2E-21
score: 78.5
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 153..258
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 25..177
IPR011707Multicopper oxidase, N-termianlPFAMPF07732Cu-oxidase_3coord: 37..150
e-value: 8.2E-38
score: 129.1
IPR045087Multicopper oxidasePANTHERPTHR11709MULTI-COPPER OXIDASEcoord: 17..261
NoneNo IPR availablePANTHERPTHR11709:SF267MULTI-COPPER OXIDASE TYPE I FAMILY PROTEIN-RELATEDcoord: 17..261
IPR034273Ascorbate oxidase homologue, first cupredoxin domainCDDcd13846CuRO_1_AAO_like_1coord: 32..147
e-value: 3.99069E-75
score: 223.053

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0001315.1Lag0001315.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005507 copper ion binding
molecular_function GO:0008447 L-ascorbate oxidase activity