Lag0001280 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0001280
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionRNase H domain-containing protein
Locationchr4: 28452448 .. 28452981 (-)
RNA-Seq ExpressionLag0001280
SyntenyLag0001280
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTATGAGTATGTGTGTGTATATATACATATATACACATGTATGTGTATTTGTGTGTATATATACACATGTATTTATATACATGTATGTGTACGTGTATGAGTATCACCTATCCCTGATGCTAATATTAGAAGCCGGTGGATTTCGAGTTACCTGGAAGATTTCCTGAAGGCTAATCAGAAATATGTTCCTCATGCGATTTCCTCTTCCAAGCAGAGGCGACCTCCGTTGAATACGAATCAAGGAGCCTCCCCTTCTCAGCAAATCAGACCTTTTTTGAGTAATCGATGGTTGCCTCCTCCATGCAATACTCGGAAGTTAAACTCTGATATTTCTAGGGTTTCGTCTCCCTCTTCGATTGGAGTCGGTTTGATTATTCGAGATTGGGAAAGGAGTATCAGAGCCGCTGCAAGTCTTTTTGTGGATTTCAATTTTGAGACTCATTTGGCTGAGATTTGGGCTATTTTGGAAGGCTTAAAGTGGGCCTTATCCCTAGGCTATATGAGGCTTATTGTGGAGTTTGATAGCTATTGA

mRNA sequence

ATGTATGAAAGCCGGTGGATTTCGAGTTACCTGGAAGATTTCCTGAAGGCTAATCAGAAATATGTTCCTCATGCGATTTCCTCTTCCAAGCAGAGGCGACCTCCGTTGAATACGAATCAAGGAGCCTCCCCTTCTCAGCAAATCAGACCTTTTTTGAGTAATCGATGGTTGCCTCCTCCATGCAATACTCGGAAGTTAAACTCTGATATTTCTAGGGTTTCGTCTCCCTCTTCGATTGGAGTCGGTTTGATTATTCGAGATTGGGAAAGGAGTATCAGAGCCGCTGCAAGTCTTTTTGTGGATTTCAATTTTGAGACTCATTTGGCTGAGATTTGGGCTATTTTGGAAGGCTTAAAGTGGGCCTTATCCCTAGGCTATATGAGGCTTATTGTGGAGTTTGATAGCTATTGA

Coding sequence (CDS)

ATGTATGAAAGCCGGTGGATTTCGAGTTACCTGGAAGATTTCCTGAAGGCTAATCAGAAATATGTTCCTCATGCGATTTCCTCTTCCAAGCAGAGGCGACCTCCGTTGAATACGAATCAAGGAGCCTCCCCTTCTCAGCAAATCAGACCTTTTTTGAGTAATCGATGGTTGCCTCCTCCATGCAATACTCGGAAGTTAAACTCTGATATTTCTAGGGTTTCGTCTCCCTCTTCGATTGGAGTCGGTTTGATTATTCGAGATTGGGAAAGGAGTATCAGAGCCGCTGCAAGTCTTTTTGTGGATTTCAATTTTGAGACTCATTTGGCTGAGATTTGGGCTATTTTGGAAGGCTTAAAGTGGGCCTTATCCCTAGGCTATATGAGGCTTATTGTGGAGTTTGATAGCTATTGA

Protein sequence

MYESRWISSYLEDFLKANQKYVPHAISSSKQRRPPLNTNQGASPSQQIRPFLSNRWLPPPCNTRKLNSDISRVSSPSSIGVGLIIRDWERSIRAAASLFVDFNFETHLAEIWAILEGLKWALSLGYMRLIVEFDSY
Homology
BLAST of Lag0001280 vs. NCBI nr
Match: XP_022131661.1 (uncharacterized protein LOC111004786 [Momordica charantia])

HSP 1 Score: 69.7 bits (169), Expect = 2.2e-08
Identity = 49/129 (37.98%), Postives = 64/129 (49.61%), Query Frame = 0

Query: 6   WISSYLEDFLKANQKYVPHAISSSKQRRPPLNTNQGASPSQQIRPFLSNRWLPPPCNTRK 65
           WISSY +++ +A +                   N+  SP Q   P  S RWLPP  +  K
Sbjct: 84  WISSYFQNYSQAQE-------------------NKRISPQQSSVPPPSCRWLPPRDSAMK 143

Query: 66  LNSDISRVSSPSSIGVGLIIRDWERSIRAAASLFVDFNFETHLAEIWAILEGLKWALSLG 125
           +NSD +  S  +S G+GLIIRD    +  A S+F+        AEI  ILE LK A S  
Sbjct: 144 VNSDAACRS--TSTGLGLIIRDHFGVLLVAKSMFLPMPLNPLFAEIRGILEALKLAASRS 191

Query: 126 YMRLIVEFD 135
           Y RL+VE D
Sbjct: 204 YTRLVVESD 191

BLAST of Lag0001280 vs. NCBI nr
Match: XP_030495196.1 (uncharacterized protein LOC115710989 [Cannabis sativa])

HSP 1 Score: 65.5 bits (158), Expect = 4.1e-07
Identity = 44/126 (34.92%), Postives = 61/126 (48.41%), Query Frame = 0

Query: 10   YLEDFLKANQKYVPHAISSSKQRRPPLNTNQGASPSQQIRPFLSNRWLPPPCNTRKLNSD 69
            YL+++  A  KY           RPP  ++Q  SPS       S  W PPP  T KLN D
Sbjct: 939  YLDNYRAAQLKY-----------RPPATSSQVPSPSTA----PSAPWQPPPMGTLKLNID 998

Query: 70   ISRVSSPSSIGVGLIIRDWERSIRAAASLFVDFNFETHLAEIWAILEGLKWALSLGYMRL 129
             +   + S IGVG +IR++   + AA S+ +  NF +H  E  A+  G+ W L       
Sbjct: 999  AAFDKTSSKIGVGAVIRNYVGQVIAALSMPIVGNFASHEMEAKAMFHGINWVLQQQLSIS 1049

Query: 130  IVEFDS 136
            IVE D+
Sbjct: 1059 IVETDA 1049

BLAST of Lag0001280 vs. NCBI nr
Match: KAA3466619.1 (reverse transcriptase [Gossypium australe])

HSP 1 Score: 64.3 bits (155), Expect = 9.2e-07
Identity = 32/83 (38.55%), Postives = 48/83 (57.83%), Query Frame = 0

Query: 54  NRWLPPPCNTRKLNSDISRVSSPSSIGVGLIIRDWERSIRAAASLFVDFNFETHLAEIWA 113
           NRW+PPP    K+N D +        G+G+IIRD E  +   AS+ +D   E ++ E WA
Sbjct: 129 NRWIPPPMGLHKVNFDGAFDGDGKKGGIGVIIRDNEGFVWGGASIKIDDVMEGNINEAWA 188

Query: 114 ILEGLKWALSLGYMRLIVEFDSY 137
            ++ LK A  +GY R+I+E D +
Sbjct: 189 AVKALKVAQEMGYRRIILESDCF 211

BLAST of Lag0001280 vs. NCBI nr
Match: XP_040967151.1 (uncharacterized protein LOC107961535 isoform X1 [Gossypium hirsutum])

HSP 1 Score: 63.5 bits (153), Expect = 1.6e-06
Identity = 31/83 (37.35%), Postives = 48/83 (57.83%), Query Frame = 0

Query: 54  NRWLPPPCNTRKLNSDISRVSSPSSIGVGLIIRDWERSIRAAASLFVDFNFETHLAEIWA 113
           NRW+PPP    K+N D +        G+G+IIRD E  +   A++ +D   E ++ E WA
Sbjct: 142 NRWIPPPMGLHKVNFDGAFDGDGKKGGIGVIIRDNEGFVWGGAAIKIDDVMERNINEAWA 201

Query: 114 ILEGLKWALSLGYMRLIVEFDSY 137
            ++ LK A  +GY R+I+E D +
Sbjct: 202 AVKALKVAQEMGYRRIILESDCF 224

BLAST of Lag0001280 vs. NCBI nr
Match: XP_016753135.1 (uncharacterized protein LOC107961535 isoform X2 [Gossypium hirsutum])

HSP 1 Score: 63.5 bits (153), Expect = 1.6e-06
Identity = 31/83 (37.35%), Postives = 48/83 (57.83%), Query Frame = 0

Query: 54  NRWLPPPCNTRKLNSDISRVSSPSSIGVGLIIRDWERSIRAAASLFVDFNFETHLAEIWA 113
           NRW+PPP    K+N D +        G+G+IIRD E  +   A++ +D   E ++ E WA
Sbjct: 92  NRWIPPPMGLHKVNFDGAFDGDGKKGGIGVIIRDNEGFVWGGAAIKIDDVMERNINEAWA 151

Query: 114 ILEGLKWALSLGYMRLIVEFDSY 137
            ++ LK A  +GY R+I+E D +
Sbjct: 152 AVKALKVAQEMGYRRIILESDCF 174

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022131661.12.2e-0837.98uncharacterized protein LOC111004786 [Momordica charantia][more]
XP_030495196.14.1e-0734.92uncharacterized protein LOC115710989 [Cannabis sativa][more]
KAA3466619.19.2e-0738.55reverse transcriptase [Gossypium australe][more]
XP_040967151.11.6e-0637.35uncharacterized protein LOC107961535 isoform X1 [Gossypium hirsutum][more]
XP_016753135.11.6e-0637.35uncharacterized protein LOC107961535 isoform X2 [Gossypium hirsutum][more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 64..136
e-value: 4.3E-5
score: 25.6
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 77..136
e-value: 8.2E-9
score: 35.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 26..52
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 26..56
IPR044730Ribonuclease H-like domain, plant typeCDDcd06222RNase_H_likecoord: 66..135
e-value: 1.06443E-11
score: 56.1684

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0001280.1Lag0001280.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity