Lag0001232 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0001232 vs. NCBI nr
Match: XP_022149385.1 (uncharacterized protein LOC111017816 [Momordica charantia])

HSP 1 Score: 431.4 bits (1108), Expect = 1.0e-116
Identity = 226/456 (49.56%), Postives = 285/456 (62.50%), Query Frame = 0

Query: 27  VTSRIHPSVSDEMNSSLVRPFQREEVLRSLNQIHPNKTPGPDGLFGAFYQHSWSVVGVDV 86
           V   I   V +  N  L++ F  E+V  +  QI P+K  GPDG  GAFY+  W VVG+DV
Sbjct: 59  VLGEIGVEVINRQNQLLLKWFSHEKVELAFRQIGPHKASGPDGFSGAFYRSFWDVVGLDV 118

Query: 87  VNCCLNILNNKVSPAPLNEMMIVLIPKKKSPKCITEYRPISLCNVSYKIVSKVLVNRMKG 146
           ++CCL +LNN VSPAPLNE +I LIPK + P  ITE+RPISLCNV YKI+ K +VNRMK 
Sbjct: 119 IHCCLEVLNNMVSPAPLNETLIALIPKVECPWRITEFRPISLCNVCYKIILKAIVNRMKR 178

Query: 147 ILNELISHNQSAFIPRRCVVDNVILGYECIHALKGRVGLPGEDDAENGVCCIVGGVLCVE 206
           +L  +IS NQSAF+P RCVVDN I+GYEC+H+++         DA  G C  +GG     
Sbjct: 179 LLGLIISCNQSAFVPGRCVVDNAIIGYECVHSIR--------SDASYGFCWCMGGADYAL 238

Query: 207 GLSSRLHETEGARAITGLRIVCGCPPIFHLFFANDSLLFFRAKEGEARAVRNILLRYERA 266
           G      E   + A  G+ ++ G P I HLFFA+DSL FFRA   EA  V+ IL  Y++A
Sbjct: 239 GFVLFAVEGGLSEASLGVHLISGGPHISHLFFADDSLFFFRASTVEADRVKGILGAYDQA 298

Query: 267 SGQTINFDKSIISFSSSTVVGVQSQIQ---GWKG-KLFSVGGREVLLKSIVQAIPCYTMN 326
           SGQTINF+KS+ SFS +T + V   ++   G +G   F   GR+  LK+I+QAIP Y+MN
Sbjct: 299 SGQTINFEKSVTSFSPNTPLSVMIVVESSIGLEGVPFFQFVGRKYSLKAIIQAIPSYSMN 358

Query: 327 CFRLPKKLVQDISTTMARFWWNGDTEDKRIHW-----------------GRYFPSGDFLD 386
           CF+LP  L+ D S  MARFWW    ED RIHW                 GRYF +  FLD
Sbjct: 359 CFKLPVGLLHDTSMAMARFWWGEFNEDNRIHWGWRIMRDSSSLLALALKGRYFKNKTFLD 418

Query: 387 AGVGSRPSYIWRSLVWGRELLGKGIRWRIGNGERVSVYGSNWIPDDVCFKVRSPVTLGLD 446
           A VGS PSYIWRSL+ GR +L  G+RWR+G+G  V +YGSNWIP D    V SP  L L 
Sbjct: 419 ADVGSNPSYIWRSLLRGRSILQSGLRWRVGDGLNVPIYGSNWIPRDGNLHVHSPPRLPLS 478

Query: 447 SRVTDLLNASGLWNEELLRHHFSPREVRSILTIPLG 462
           S V+ L++ASG WNE ++R  F   E   IL IP+G
Sbjct: 479 SIVSTLISASGGWNEGIIRGAFDEGEASLILRIPVG 506

BLAST of Lag0001232 vs. NCBI nr
Match: XP_023881891.1 (uncharacterized protein LOC111994244 [Quercus suber])

HSP 1 Score: 360.1 bits (923), Expect = 2.8e-95
Identity = 212/607 (34.93%), Postives = 310/607 (51.07%), Query Frame = 0

Query: 9   YFENIFSTSSPSEGEIDQVTSRIHPSVSDEMNSSLVRPFQREEVLRSLNQIHPNKTPGPD 68
           YF+ I+S+S P+   I +V   I  +V++EMN SL++ F REE+  +LNQ+HP K PGPD
Sbjct: 232 YFQTIYSSSVPT--RISEVLDAIPTTVTEEMNHSLIQEFTREEIETALNQMHPTKAPGPD 291

Query: 69  GLFGAFYQHSWSVVGVDVVNCCLNILNNKVSPAPLNEMMIVLIPKKKSPKCITEYRPISL 128
           G+   F+Q  W++VG D+V   L++LN+ +S   +N+  I L+PK K+P  ++++RPISL
Sbjct: 292 GMSAIFFQKYWNIVGNDIVCMVLDVLNSNMSMVEINKTNITLVPKIKNPTKMSDFRPISL 351

Query: 129 CNVSYKIVSKVLVNRMKGILNELISHNQSAFIPRRCVVDNVILGYECIHAL----KGRVG 188
           CNV YK++SKVL NR+K IL ++IS NQSAF+  R + DNV++ +E +H L    +G+ G
Sbjct: 352 CNVVYKLISKVLANRLKNILPQIISENQSAFLSGRLITDNVLVAFELMHYLEHKKEGKEG 411

Query: 189 L--------PGEDDAENG-------------------VCCIV---------GG------- 248
                       D  E G                   + CI          GG       
Sbjct: 412 FAAIKLDMSKAYDRVEWGFIKQVMEKMGFHEKWIKLVMHCITSVSYSILVNGGAYGSITP 471

Query: 249 ---------------VLCVEGLSSRLHETEGARAITGLRIVCGCPPIFHLFFANDSLLFF 308
                          +LC +G SS L++      I+G+ I  GCP I HLFFA+DSLLF 
Sbjct: 472 TRGLRQGDPISPYIFLLCADGFSSLLNDVARKLRISGVSICRGCPKITHLFFADDSLLFC 531

Query: 309 RAKEGEARAVRNILLRYERASGQTINFDKSIISFSSST---------------------- 368
           +A   E + + +IL  YE ASGQ IN DKS + FS++T                      
Sbjct: 532 KANSQECQTLIDILQLYEDASGQKINVDKSSVFFSNNTPDEKRCEVLRMLGHMQDTRHKK 591

Query: 369 ------VVG-------------VQSQIQGWKGKLFSVGGREVLLKSIVQAIPCYTMNCFR 428
                 ++G             V+ ++ GWK KL SVGGRE+L+K++ QAIP YTM+CF+
Sbjct: 592 YLGLPSIIGKSKVEIFAEVKERVERKLSGWKEKLLSVGGREILIKAVAQAIPTYTMSCFQ 651

Query: 429 LPKKLVQDISTTMARFWWNGDTEDKRIHW------------------------------- 463
           +PK L ++I   M RFWW    ++ +I W                               
Sbjct: 652 IPKTLCEEIEAMMRRFWWGQRGQESKIAWVSWKKLCKAKKNGGMGFRNLQAFNLAMLAKQ 711

BLAST of Lag0001232 vs. NCBI nr
Match: XP_023901742.1 (uncharacterized protein LOC112013579 [Quercus suber])

HSP 1 Score: 359.4 bits (921), Expect = 4.8e-95
Identity = 211/605 (34.88%), Postives = 294/605 (48.60%), Query Frame = 0

Query: 9   YFENIFSTSSPSEGEIDQVTSRIHPSVSDEMNSSLVRPFQREEVLRSLNQIHPNKTPGPD 68
           YFENI+++SSP+   I++V + I   V+DEMNS L + F  EEVL++L Q+HP K PGPD
Sbjct: 241 YFENIYTSSSPT--GINEVINAIPRRVTDEMNSELSKTFTSEEVLKALKQLHPTKAPGPD 300

Query: 69  GLFGAFYQHSWSVVGVDVVNCCLNILNNKVSPAPLNEMMIVLIPKKKSPKCITEYRPISL 128
           G+   F+ + W +VG  + N  LN+LN+ +    +N+  I LIPK   P  +TE+RPISL
Sbjct: 301 GMSATFFHNYWDIVGPSITNMVLNVLNSNLPMTEINKTNISLIPKTNQPVRMTEFRPISL 360

Query: 129 CNVSYKIVSKVLVNRMKGILNELISHNQSAFIPRRCVVDNVILGYECIHALKGR------ 188
           CN +YKI+SKVL NR K IL  +IS NQSAF P R + DNV++ +E +H L  +      
Sbjct: 361 CNTTYKIISKVLANRFKAILPNIISENQSAFTPNRLITDNVLVAFEFMHYLNHKNEGKEN 420

Query: 189 ---VGLPGEDDAENGVCCIVGGV------------------------------------- 248
              + L      +      + GV                                     
Sbjct: 421 YMSIKLDMSKAFDRVEWSFIKGVMEKLGFMEKWIHLIMNCVSSVSYSVLINGEAYGNITP 480

Query: 249 ----------------LCVEGLSSRLHETEGARAITGLRIVCGCPPIFHLFFANDSLLFF 308
                           LC EGLS+ +HE    + I G+ I  GCP I HLFFA+DSLLF 
Sbjct: 481 SRGIRQGDPLSPCLFLLCAEGLSALIHEAARNQQINGISICRGCPLITHLFFADDSLLFC 540

Query: 309 RAKEGEARAVRNILLRYERASGQTINFDKSIISFSSST---------------------- 368
           +AKE E  A+ +IL RYE ASGQ IN DKS + FS +T                      
Sbjct: 541 KAKEQECHALVSILNRYEEASGQKINTDKSSVFFSPNTSQELRESIFNILGPMQDSRHSK 600

Query: 369 ------VVG-------------VQSQIQGWKGKLFSVGGREVLLKSIVQAIPCYTMNCFR 428
                 ++G             V  ++ GWKGKL S+GGRE+L+K++ QA+P YTM+CF+
Sbjct: 601 YLGLPSIIGKSKAQVFAEVKDRVAKKLAGWKGKLLSIGGREILIKAVAQAVPTYTMSCFQ 660

Query: 429 LPKKLVQDISTTMARFWWNGDTEDKRIHW------------------------------- 461
           LPK L QD+ + M  FWW    ++ +I W                               
Sbjct: 661 LPKTLCQDLESLMRSFWWGQKDKENKIAWVSWRKMCRSKLHGGMGFRNIQAFNLAMLAKQ 720

BLAST of Lag0001232 vs. NCBI nr
Match: XP_023878301.1 (uncharacterized protein LOC111990748 [Quercus suber])

HSP 1 Score: 357.1 bits (915), Expect = 2.4e-94
Identity = 213/607 (35.09%), Postives = 304/607 (50.08%), Query Frame = 0

Query: 9   YFENIFSTSSPSEGEIDQVTSRIHPSVSDEMNSSLVRPFQREEVLRSLNQIHPNKTPGPD 68
           YF NI+S+S PS  +I++VT  I   V++EMN SL+R F +EEV  +L QIHPNK PGPD
Sbjct: 356 YFNNIYSSSHPS--QIEEVTEAIPFKVTEEMNESLIREFTKEEVAVALKQIHPNKAPGPD 415

Query: 69  GLFGAFYQHSWSVVGVDVVNCCLNILNNKVSPAPLNEMMIVLIPKKKSPKCITEYRPISL 128
           G+   F+Q  WS+VG +V +  LN+LN+ +    LN+  I LIPK  +PK +T++RPISL
Sbjct: 416 GMSAVFFQKYWSIVGNNVTDMVLNVLNHNLPIPELNKTNISLIPKTNNPKRMTDFRPISL 475

Query: 129 CNVSYKIVSKVLVNRMKGILNELISHNQSAFIPRRCVVDNVILGYECIHALKGRV----- 188
           CNV YK++SK+L NR+K +L  +IS NQSAF   R + DNV++ +E +H L  +      
Sbjct: 476 CNVVYKLISKILANRLKPLLPHIISENQSAFTSDRLITDNVLVAFELMHYLDHKTAGKEG 535

Query: 189 -----------------GLPGEDDAENGVC---------CI--------VGGV------- 248
                            G   +   + G C         CI        + GV       
Sbjct: 536 FMAIKLDMSKAFDRVEWGFIAKVMEQMGFCNRWRDLVMQCITSVSYSILINGVAHGNIYP 595

Query: 249 ----------------LCVEGLSSRLHETEGARAITGLRIVCGCPPIFHLFFANDSLLFF 308
                           LC EGLS+ +++    + ITG+ I  GCP + HLFFA+DS+LF 
Sbjct: 596 SRGLRQGDPLSPSLFLLCAEGLSALINQAARNKLITGISINRGCPKVTHLFFADDSILFC 655

Query: 309 RAKEGEARAVRNILLRYERASGQTINFDKSIISFSSST---------------------- 368
           +A   E   +R+IL +YE ASGQ IN DKS I FS +T                      
Sbjct: 656 KAAYEECHLLRSILGQYEEASGQKINTDKSSIFFSPNTAQETRDEIFNILGPMQNSRHTK 715

Query: 369 ------VVG-------------VQSQIQGWKGKLFSVGGREVLLKSIVQAIPCYTMNCFR 428
                 ++G             V  ++ GWKGKL S+GG+E+L+K++ QAIP YTM+CF 
Sbjct: 716 YLGLPSLIGRSKSQVFAMLKEKVGHKLAGWKGKLLSMGGKEILIKAVAQAIPTYTMSCFL 775

Query: 429 LPKKLVQDISTTMARFWWNGDTEDKRIHW------------------------------- 463
           LP+ L  D+   M  FWW    ++ ++ W                               
Sbjct: 776 LPQGLCDDMERMMKNFWWGQRNQETKMGWISWKRMCNSKASGGLGFRNLKAFNLAMLAKQ 835

BLAST of Lag0001232 vs. NCBI nr
Match: XP_023912601.1 (uncharacterized protein LOC112024185 [Quercus suber])

HSP 1 Score: 347.1 bits (889), Expect = 2.5e-91
Identity = 205/605 (33.88%), Postives = 290/605 (47.93%), Query Frame = 0

Query: 9   YFENIFSTSSPSEGEIDQVTSRIHPSVSDEMNSSLVRPFQREEVLRSLNQIHPNKTPGPD 68
           YFENI+++SSP+   I++V + I   V+DEMNS L + F  EEVL++L Q+HP K P PD
Sbjct: 173 YFENIYTSSSPT--GINEVINAIPRRVTDEMNSELTKTFTSEEVLKALKQLHPTKAPRPD 232

Query: 69  GLFGAFYQHSWSVVGVDVVNCCLNILNNKVSPAPLNEMMIVLIPKKKSPKCITEYRPISL 128
           G+   F+ + W +VG  + N  LN+LN+ +    +N+  I LIPK   P  +TE+RPISL
Sbjct: 233 GMSAIFFHNYWDIVGPSITNMVLNVLNSNLPMTEINKTNISLIPKTNQPVRMTEFRPISL 292

Query: 129 CNVSYKIVSKVLVNRMKGILNELISHNQSAFIPRRCVVDNVILGYECIHALKGR------ 188
           CN +YKI+SKVL NR K IL  +IS NQSAF P R + DNV++ ++ +H L  +      
Sbjct: 293 CNTTYKIISKVLANRFKAILPNIISENQSAFTPNRLITDNVLVAFKFMHYLNHKNEGKEN 352

Query: 189 ---VGLPGEDDAENGVCCIVGGV------------------------------------- 248
              + L      +      + GV                                     
Sbjct: 353 YMSIKLDMSKAFDRVEWSFIKGVMEKLGFMEKWIHLIMNCVSSVSYSVLINGEAFGNIAP 412

Query: 249 ----------------LCVEGLSSRLHETEGARAITGLRIVCGCPPIFHLFFANDSLLFF 308
                           LC +GLS+ +HE    + I G+ I  GCP I HLFFA+DSLLF 
Sbjct: 413 SRGIRQGDPLSPCLFLLCAKGLSALIHEATRNQQINGISICRGCPLITHLFFADDSLLFC 472

Query: 309 RAKEGEARAVRNILLRYERASGQTINFDKSIISFSSST---------------------- 368
           +AKE E  A+ +IL RYE AS Q IN DKS + FS +T                      
Sbjct: 473 KAKEQECHALVSILHRYEEASRQKINTDKSSVFFSPNTSQELRESIFNILGPMQDSRHSK 532

Query: 369 ------VVG-------------VQSQIQGWKGKLFSVGGREVLLKSIVQAIPCYTMNCFR 428
                 ++G             V  ++ GWKGKL S+GGRE+L+K++ Q +P YTM+CF+
Sbjct: 533 YLGLPSIIGKSKAQMFAEVKDRVAKKLAGWKGKLLSIGGREILIKAVAQTVPTYTMSCFQ 592

Query: 429 LPKKLVQDISTTMARFWWNGDTEDKRIHW------------------------------- 461
           LPK L QD+ + M  FWW    ++ +I W                               
Sbjct: 593 LPKTLCQDLESLMRSFWWGQKDKENKIAWVSWRKMCRSKLHGGMGFRNIQAFNLAMLAKQ 652

BLAST of Lag0001232 vs. ExPASy Swiss-Prot
Match: P14381 (Transposon TX1 uncharacterized 149 kDa protein OS=Xenopus laevis OX=8355 PE=4 SV=1)

HSP 1 Score: 94.0 bits (232), Expect = 4.9e-18
Identity = 55/177 (31.07%), Postives = 90/177 (50.85%), Query Frame = 0

Query: 9   YFENIFSTSSPSEGEIDQVTSRIHPSVSDEMNSSLVRPFQREEVLRSLNQIHPNKTPGPD 68
           +++N+FS    S    +++   + P VS+     L  P   +E+ ++L  +  NK+PG D
Sbjct: 412 FYQNLFSPDPISPDACEELWDGL-PVVSERRKERLETPITLDELSQALRLMPHNKSPGLD 471

Query: 69  GLFGAFYQHSWSVVGVDVVNCCLNILNNKVSPAPLNEMMIVLIPKKKSPKCITEYRPISL 128
           GL   F+Q  W  +G D              P      ++ L+PKK   + I  +RP+SL
Sbjct: 472 GLTIEFFQFFWDTLGPDFHRVLTEAFKKGELPLSCRRAVLSLLPKKGDLRLIKNWRPVSL 531

Query: 129 CNVSYKIVSKVLVNRMKGILNELISHNQSAFIPRRCVVDNVILGYECIHALKGRVGL 186
            +  YKIV+K +  R+K +L E+I  +QS  +P R + DNV L  + +H  + R GL
Sbjct: 532 LSTDYKIVAKAISLRLKSVLAEVIHPDQSYTVPGRTIFDNVFLIRDLLHFAR-RTGL 586

BLAST of Lag0001232 vs. ExPASy Swiss-Prot
Match: P08548 (LINE-1 reverse transcriptase homolog OS=Nycticebus coucang OX=9470 PE=4 SV=1)

HSP 1 Score: 89.0 bits (219), Expect = 1.6e-16
Identity = 53/159 (33.33%), Postives = 81/159 (50.94%), Query Frame = 0

Query: 5   LIEGYFENIFSTSSPSEGEIDQVTSRIH-PSVSDEMNSSLVRPFQREEVLRSLNQIHPNK 64
           ++  Y++ ++S    +  EIDQ     H P +S +    L RP    E+  ++  +   K
Sbjct: 409 ILNEYYKKLYSHKYENLKEIDQYLEACHLPRLSQKEVEMLNRPISSSEIASTIQNLPKKK 468

Query: 65  TPGPDGLFGAFYQHSWSVVGVDVVNCCLNILNNKVSPAPLNEMMIVLIPKK-KSPKCITE 124
           +PGPDG    FYQ     +   ++N   NI    + P    E  I LIPK  K P     
Sbjct: 469 SPGPDGFTSEFYQTFKEELVPILLNLFQNIEKEGILPNTFYEANITLIPKPGKDPTRKEN 528

Query: 125 YRPISLCNVSYKIVSKVLVNRMKGILNELISHNQSAFIP 162
           YRPISL N+  KI++K+L NR++  + ++I H+Q  FIP
Sbjct: 529 YRPISLMNIDAKILNKILTNRIQQHIKKIIHHDQVGFIP 567

BLAST of Lag0001232 vs. ExPASy Swiss-Prot
Match: P93295 (Uncharacterized mitochondrial protein AtMg00310 OS=Arabidopsis thaliana OX=3702 GN=AtMg00310 PE=4 SV=1)

HSP 1 Score: 84.0 bits (206), Expect = 5.1e-15
Identity = 42/146 (28.77%), Postives = 61/146 (41.78%), Query Frame = 0

Query: 315 AIPCYTMNCFRLPKKLVQDISTTMARFWWNGDTEDKRIHW-------------------- 374
           A+P Y M+CFRL K L + +++ M  FWW+     ++I W                    
Sbjct: 2   ALPVYAMSCFRLSKLLCKKLTSAMTEFWWSSCENKRKISWVAWQKLCKSKEDDGGLGFRD 61

Query: 375 -----------------------------GRYFPSGDFLDAGVGSRPSYIWRSLVWGREL 412
                                         RYFP    ++  VG+RPSY WRS++ GREL
Sbjct: 62  LGWFNQALLAKQSFRIIHQPHTLLSRLLRSRYFPHSSMMECSVGTRPSYAWRSIIHGREL 121

BLAST of Lag0001232 vs. ExPASy Swiss-Prot
Match: O00370 (LINE-1 retrotransposable element ORF2 protein OS=Homo sapiens OX=9606 PE=1 SV=1)

HSP 1 Score: 79.3 bits (194), Expect = 1.3e-13
Identity = 49/158 (31.01%), Postives = 82/158 (51.90%), Query Frame = 0

Query: 6   IEGYFENIFSTSSPSEGEIDQ-VTSRIHPSVSDEMNSSLVRPFQREEVLRSLNQIHPNKT 65
           I  Y++++++    +  E+D  + +   P ++ E   SL RP    E++  +N +   K+
Sbjct: 411 IREYYKHLYANKLENLEEMDTFLDTYTLPRLNQEEVESLNRPITGSEIVAIINSLPTKKS 470

Query: 66  PGPDGLFGAFYQHSWSVVGVDVVNCCLNILNNKVSPAPLNEMMIVLIPKKKSPKCITE-Y 125
           PGPDG    FYQ     +   ++    +I    + P    E  I+LIPK        E +
Sbjct: 471 PGPDGFTAEFYQRYKEELVPFLLKLFQSIEKEGILPNSFYEASIILIPKPGRDTTKKENF 530

Query: 126 RPISLCNVSYKIVSKVLVNRMKGILNELISHNQSAFIP 162
           RPISL N+  KI++K+L NR++  + +LI H+Q  FIP
Sbjct: 531 RPISLMNIDAKILNKILANRIQQHIKKLIHHDQVGFIP 568

BLAST of Lag0001232 vs. ExPASy Swiss-Prot
Match: P11369 (LINE-1 retrotransposable element ORF2 protein OS=Mus musculus OX=10090 GN=Pol PE=1 SV=2)

HSP 1 Score: 75.1 bits (183), Expect = 2.4e-12
Identity = 51/163 (31.29%), Postives = 83/163 (50.92%), Query Frame = 0

Query: 6   IEGYFENIFSTSSPSEGEIDQVTSRIH-PSVSDEMNSSLVRPFQREEVLRSLNQIHPNKT 65
           I  +++ ++ST   +  E+D+   R   P ++ +    L  P   +E+   +N +   K+
Sbjct: 418 IRSFYKRLYSTKLENLDEMDKFLDRYQVPKLNQDQVDHLNSPISPKEIEAVINSLPTKKS 477

Query: 66  PGPDGLFGAFYQHSWSVVGVDVVNCCLNILNNKVS-----PAPLNEMMIVLIPK-KKSPK 125
           PGPDG    FYQ        D++   L+ L +K+      P    E  I LIPK +K P 
Sbjct: 478 PGPDGFSAEFYQ----TFKEDLIP-ILHKLFHKIEVEGTLPNSFYEATITLIPKPQKDPT 537

Query: 126 CITEYRPISLCNVSYKIVSKVLVNRMKGILNELISHNQSAFIP 162
            I  +RPISL N+  KI++K+L NR++  +  +I  +Q  FIP
Sbjct: 538 KIENFRPISLMNIDAKILNKILANRIQEHIKAIIHPDQVGFIP 575

BLAST of Lag0001232 vs. ExPASy TrEMBL
Match: A0A6J1D5K1 (uncharacterized protein LOC111017816 OS=Momordica charantia OX=3673 GN=LOC111017816 PE=4 SV=1)

HSP 1 Score: 431.4 bits (1108), Expect = 4.8e-117
Identity = 226/456 (49.56%), Postives = 285/456 (62.50%), Query Frame = 0

Query: 27  VTSRIHPSVSDEMNSSLVRPFQREEVLRSLNQIHPNKTPGPDGLFGAFYQHSWSVVGVDV 86
           V   I   V +  N  L++ F  E+V  +  QI P+K  GPDG  GAFY+  W VVG+DV
Sbjct: 59  VLGEIGVEVINRQNQLLLKWFSHEKVELAFRQIGPHKASGPDGFSGAFYRSFWDVVGLDV 118

Query: 87  VNCCLNILNNKVSPAPLNEMMIVLIPKKKSPKCITEYRPISLCNVSYKIVSKVLVNRMKG 146
           ++CCL +LNN VSPAPLNE +I LIPK + P  ITE+RPISLCNV YKI+ K +VNRMK 
Sbjct: 119 IHCCLEVLNNMVSPAPLNETLIALIPKVECPWRITEFRPISLCNVCYKIILKAIVNRMKR 178

Query: 147 ILNELISHNQSAFIPRRCVVDNVILGYECIHALKGRVGLPGEDDAENGVCCIVGGVLCVE 206
           +L  +IS NQSAF+P RCVVDN I+GYEC+H+++         DA  G C  +GG     
Sbjct: 179 LLGLIISCNQSAFVPGRCVVDNAIIGYECVHSIR--------SDASYGFCWCMGGADYAL 238

Query: 207 GLSSRLHETEGARAITGLRIVCGCPPIFHLFFANDSLLFFRAKEGEARAVRNILLRYERA 266
           G      E   + A  G+ ++ G P I HLFFA+DSL FFRA   EA  V+ IL  Y++A
Sbjct: 239 GFVLFAVEGGLSEASLGVHLISGGPHISHLFFADDSLFFFRASTVEADRVKGILGAYDQA 298

Query: 267 SGQTINFDKSIISFSSSTVVGVQSQIQ---GWKG-KLFSVGGREVLLKSIVQAIPCYTMN 326
           SGQTINF+KS+ SFS +T + V   ++   G +G   F   GR+  LK+I+QAIP Y+MN
Sbjct: 299 SGQTINFEKSVTSFSPNTPLSVMIVVESSIGLEGVPFFQFVGRKYSLKAIIQAIPSYSMN 358

Query: 327 CFRLPKKLVQDISTTMARFWWNGDTEDKRIHW-----------------GRYFPSGDFLD 386
           CF+LP  L+ D S  MARFWW    ED RIHW                 GRYF +  FLD
Sbjct: 359 CFKLPVGLLHDTSMAMARFWWGEFNEDNRIHWGWRIMRDSSSLLALALKGRYFKNKTFLD 418

Query: 387 AGVGSRPSYIWRSLVWGRELLGKGIRWRIGNGERVSVYGSNWIPDDVCFKVRSPVTLGLD 446
           A VGS PSYIWRSL+ GR +L  G+RWR+G+G  V +YGSNWIP D    V SP  L L 
Sbjct: 419 ADVGSNPSYIWRSLLRGRSILQSGLRWRVGDGLNVPIYGSNWIPRDGNLHVHSPPRLPLS 478

Query: 447 SRVTDLLNASGLWNEELLRHHFSPREVRSILTIPLG 462
           S V+ L++ASG WNE ++R  F   E   IL IP+G
Sbjct: 479 SIVSTLISASGGWNEGIIRGAFDEGEASLILRIPVG 506

BLAST of Lag0001232 vs. ExPASy TrEMBL
Match: A0A7N2L6Z9 (Reverse transcriptase domain-containing protein OS=Quercus lobata OX=97700 PE=4 SV=1)

HSP 1 Score: 354.0 bits (907), Expect = 9.8e-94
Identity = 207/605 (34.21%), Postives = 296/605 (48.93%), Query Frame = 0

Query: 9   YFENIFSTSSPSEGEIDQVTSRIHPSVSDEMNSSLVRPFQREEVLRSLNQIHPNKTPGPD 68
           YFE+I+STS+PS   +D+VT+ I   +++EMN+ L R F REE++ +L QIHP K+PGPD
Sbjct: 271 YFEDIYSTSNPS--MVDEVTAAIPTMITEEMNTELSRCFTREEIVTALKQIHPTKSPGPD 330

Query: 69  GLFGAFYQHSWSVVGVDVVNCCLNILNNKVSPAPLNEMMIVLIPKKKSPKCITEYRPISL 128
           G+   F+Q  W +VG +V N  LN+LN  +S   +N+  IVLIPK  +PK +T++RPISL
Sbjct: 331 GMSAIFFQKYWDIVGSNVSNMILNVLNYGMSLDVINKTNIVLIPKTSNPKRMTDFRPISL 390

Query: 129 CNVSYKIVSKVLVNRMKGILNELISHNQSAFIPRRCVVDNVILGYECIHALK-------- 188
           CNV YK++SK L NR+K  L  +I+ NQSAF   R + DNV++ YE +H LK        
Sbjct: 391 CNVIYKLISKTLANRLKAFLPLIITENQSAFTADRLITDNVLIAYELMHYLKHKKDGKDC 450

Query: 189 -------------------------------GRVGLPGEDDAENGVCCIVGG-------- 248
                                          G + L     +      I+ G        
Sbjct: 451 FMATKLDMSKAFDRVEWGFIERVMRKMGFNEGWISLIMRCISSVSYSVIINGEYFGNIVP 510

Query: 249 ---------------VLCVEGLSSRLHETEGARAITGLRIVCGCPPIFHLFFANDSLLFF 308
                          +LC EGLS+ LH+    + + G+ +  GCP I HLFFA+DSLLF 
Sbjct: 511 TRGLRQGDPLSPYLFLLCAEGLSALLHDAARNQLLNGISLCRGCPRITHLFFADDSLLFC 570

Query: 309 RAKEGEARAVRNILLRYERASGQTINFDKSIISFSSST---------------------- 368
           +A   E   ++ IL +YE ASGQ +N DKS I FS +T                      
Sbjct: 571 KANREECEKLKEILEKYEAASGQKVNSDKSSIFFSPNTTPELKETIFNILGPMQDSRHNK 630

Query: 369 ------VVG-------------VQSQIQGWKGKLFSVGGREVLLKSIVQAIPCYTMNCFR 428
                 ++G             V  ++ GWKGKL S GG+E+L+K++ QAIP YTM+CF 
Sbjct: 631 YLGLPSIIGRSKKLVFAEIKERVGLKLAGWKGKLLSSGGKEILIKAVAQAIPTYTMSCFL 690

Query: 429 LPKKLVQDISTTMARFWWNGDTEDKRIHW------------------------------- 461
           LPK L  ++   M  FWW    ++ ++ W                               
Sbjct: 691 LPKSLCDELEKMMRNFWWGQKNQESKVAWISWRKMCKPKSLGGLGFRNLHAFNLALLAKQ 750

BLAST of Lag0001232 vs. ExPASy TrEMBL
Match: A0A2N9FRF7 (Reverse transcriptase domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS17562 PE=4 SV=1)

HSP 1 Score: 340.5 bits (872), Expect = 1.1e-89
Identity = 201/589 (34.13%), Postives = 296/589 (50.25%), Query Frame = 0

Query: 5    LIEGYFENIFSTSSPSEGEIDQVTSRIHPSVSDEMNSSLVRPFQREEVLRSLNQIHPNKT 64
            ++E YF +IF TS P    IDQV S + P ++  MN+SL++P    EV  +L Q+HP K+
Sbjct: 693  IVERYFSDIFHTSFPQ--TIDQVVSNVEPKITPAMNNSLLKPVTSAEVHEALFQMHPTKS 752

Query: 65   PGPDGLFGAFYQHSWSVVGVDVVNCCLNILNNKVSPAPLNEMMIVLIPKKKSPKCITEYR 124
            PGPDG+   FYQ  W ++G DV    +    +      +N   I LIPK K+P+ +T++R
Sbjct: 753  PGPDGMNALFYQKFWHIIGNDVTKAIIEFFQSGKMLKSINYTHIALIPKVKAPESMTQFR 812

Query: 125  PISLCNVSYKIVSKVLVNRMKGILNELISHNQSAFIPRRCVVDNVILGYECIHAL----K 184
            PISLCNV YKI+SKVL NR+K +LN +IS NQSAF+P R + DN+++ +E +H L    K
Sbjct: 813  PISLCNVMYKIISKVLANRLKLVLNHVISGNQSAFVPERLITDNILVAFEALHYLKTKRK 872

Query: 185  GR-----VGLP-------GEDDAENGVCCIVG--------------------------GV 244
            GR     V L         E D  + + C +G                           +
Sbjct: 873  GRSTHMAVKLDMSKAYDRVEWDFLSTMMCKMGFNDRWPMGYIRPTRGIRQGDPLSPYLFL 932

Query: 245  LCVEGLSSRLHETEGARAITGLRIVCGCPPIFHLFFANDSLLFFRAKEGEARAVRNILLR 304
            +C EGL++ L     +  +TGL +  G P I HLFFA+DSLLF +A   E R + + L  
Sbjct: 933  ICAEGLTTLLQNNAASGQLTGLSLNRGGPQISHLFFADDSLLFCKATMEECRVLLSTLNV 992

Query: 305  YERASGQTINFDKSIISFSSST----------------------------VVG------- 364
            YERASGQ +N++K+ + FS++T                            ++G       
Sbjct: 993  YERASGQKLNYEKTSLFFSTNTAQATRQSICNALRTSSTGDLGKYLGLPPIIGRGKKQAF 1052

Query: 365  ------VQSQIQGWKGKLFSVGGREVLLKSIVQAIPCYTMNCFRLPKKLVQDISTTMARF 424
                  V  ++ GWKGK+ S  GREVL+KS+ QA+P YTM+CFRLP  L  ++++ + +F
Sbjct: 1053 TEIKQKVARKLHGWKGKMLSQAGREVLIKSVAQALPVYTMSCFRLPDSLCTELNSMIGKF 1112

Query: 425  WWNGDTEDKRIHW----------------------------------------------- 461
            WW     +++IHW                                               
Sbjct: 1113 WWGQKNSERKIHWQKWSKLCRKKEDEGMGFRDLALFDQALLAKQGWRLMQNPNTLLHRVL 1172

BLAST of Lag0001232 vs. ExPASy TrEMBL
Match: A0A2N9ELB0 (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS3241 PE=4 SV=1)

HSP 1 Score: 340.1 bits (871), Expect = 1.5e-89
Identity = 206/605 (34.05%), Postives = 287/605 (47.44%), Query Frame = 0

Query: 9    YFENIFSTSSPSEGEIDQVTSRIHPSVSDEMNSSLVRPFQREEVLRSLNQIHPNKTPGPD 68
            YF N+F++S+P    ID+V   + P VS +MN SL++P+  EE+ R+L QI P+K PGPD
Sbjct: 829  YFHNLFTSSNPE--SIDEVIQSVAPVVSPDMNDSLMQPYSPEEIRRALFQISPSKAPGPD 888

Query: 69   GLFGAFYQHSWSVVGVDVVNCCLNILNNKVSPAPLNEMMIVLIPKKKSPKCITEYRPISL 128
            G+   F+Q  W +VGVDV    L+ LN+      +N   I LIPK K+P+C+  +RPISL
Sbjct: 889  GMTALFFQKYWHIVGVDVSLAILDFLNSGRMLGSINFTNIALIPKVKNPECMANFRPISL 948

Query: 129  CNVSYKIVSKVLVNRMKGILNELISHNQSAFIPRRCVVDNVILGYECIHALKG-RVGLPG 188
            CNV YKIVSKVLVNRMK IL  +IS +QSAF+P R + DNVI+ +E +H LK  R G   
Sbjct: 949  CNVLYKIVSKVLVNRMKPILPRVISDSQSAFVPGRLITDNVIMAFEVLHYLKNLRSGNNA 1008

Query: 189  EDDAENG------------------------------VCCI--------VGG-------- 248
            +  A+                                + C+        V G        
Sbjct: 1009 QMAAKLDMSKAYDRVEWDYLQAILLKLGFNSSWVKLIMACVNSASYSVLVNGEAQGYIKP 1068

Query: 249  ---------------VLCVEGLSSRLHETEGARAITGLRIVCGCPPIFHLFFANDSLLFF 308
                           ++C EGLSS + + E      G+ I  G P I HLFFA+DS++F 
Sbjct: 1069 SRGLRQGDPLSPYLFLICAEGLSSLIRKAERDHMFRGVAICRGGPRISHLFFADDSVIFC 1128

Query: 309  RAKEGEARAVRNILLRYERASGQTINFDKSIISFSSST---------------------- 368
            RA   +   ++N+L  YE+ASGQ +N DK+ + FS +T                      
Sbjct: 1129 RASTSDCCVIQNLLSLYEKASGQKVNGDKTALFFSKNTPSVKKVDILSLFGTSPTTQFEK 1188

Query: 369  ------VVG-------------VQSQIQGWKGKLFSVGGREVLLKSIVQAIPCYTMNCFR 428
                  ++G             +  ++QGWK KL S  GRE L+K++VQAIP Y M+CF+
Sbjct: 1189 YLGLPPIMGRSKRYAFNDIKERIWKRLQGWKEKLLSQAGRETLIKAVVQAIPTYAMSCFK 1248

Query: 429  LPKKLVQDISTTMARFWWNGDTEDKRIHW------------------------------- 461
             P  L  +I +   RFWW     +++IHW                               
Sbjct: 1249 FPAGLCAEICSMANRFWWGQRGGERKIHWVRKDQLIRPKKEGGMGFRDLHLFNKALLARQ 1308

BLAST of Lag0001232 vs. ExPASy TrEMBL
Match: M5W5F3 (Reverse transcriptase domain-containing protein (Fragment) OS=Prunus persica OX=3760 GN=PRUPE_ppa026368mg PE=4 SV=1)

HSP 1 Score: 339.3 bits (869), Expect = 2.5e-89
Identity = 194/603 (32.17%), Postives = 289/603 (47.93%), Query Frame = 0

Query: 9   YFENIFSTSSPSEGEIDQVTSRIHPSVSDEMNSSLVRPFQREEVLRSLNQIHPNKTPGPD 68
           YF+ +FS+S     +++++ + + P ++  MN+ L++ F REE+  +L Q+ P K PG D
Sbjct: 95  YFKTLFSSSGGQ--QMERILNEVRPVITSAMNAQLLQAFTREELEHTLFQMFPTKAPGHD 154

Query: 69  GLFGAFYQHSWSVVGVDVVNCCLNILNNKVSPAPLNEMMIVLIPKKKSPKCITEYRPISL 128
           G+   F+Q  W +VG  V   CL ILN + S    N  +I LIPK K P  ++E+RPISL
Sbjct: 155 GMPALFFQKYWHIVGDKVAKKCLQILNGEGSVREFNHTLIALIPKVKMPTIVSEFRPISL 214

Query: 129 CNVSYKIVSKVLVNRMKGILNELISHNQSAFIPRRCVVDNVILGYECIHALKG------- 188
           C   YK+++K + NR+K +L+ +I+  QSAF+P R ++DNV+  +E ++ +KG       
Sbjct: 215 CTTVYKMIAKTIANRLKTVLSHVITETQSAFVPNRMILDNVMAAFEIMNTIKGVKKGRDV 274

Query: 189 ----RVGLPGEDDAENGVC--------------------CI-------------VGGV-- 248
               ++ +    D    V                     CI             VG +  
Sbjct: 275 QMALKLDMAKAYDRVEWVFLRAMMLKLGFSATWVSKVMDCISTTTFSVLWKGTPVGHIMP 334

Query: 249 ----------------LCVEGLSSRLHETEGARAITGLRIVCGCPPIFHLFFANDSLLFF 308
                           +C EG S  L   E    + G+++  G P + HL FA+DS+LF 
Sbjct: 335 QRGLRQGCPLSPYLFLICTEGFSCLLRGAERRGDLVGVQVARGAPSVTHLLFADDSILFM 394

Query: 309 RAKEGEARAVRNILLRYERASGQTINFDKSIISFSSS----------------------- 368
           +A   +  A+  +   YE  +GQ IN+ KS +S S +                       
Sbjct: 395 KATNKDCMALETLFQTYEEVTGQQINYSKSALSLSPNATRADFDMIEGVLNVPVVRCHEN 454

Query: 369 -----TVVG-------------VQSQIQGWKGKLFSVGGREVLLKSIVQAIPCYTMNCFR 428
                T+ G             +   I GWK KL S  G+E+L+K+++QAIP Y+M+CFR
Sbjct: 455 YLGLPTIAGKGRKQLFQHLKDKLWKHISGWKEKLLSRAGKEILIKAVLQAIPTYSMSCFR 514

Query: 429 LPKKLVQDISTTMARFWWNGDTEDKRIHW------------------------------- 461
           +PK L ++++  MARFWW    + + IHW                               
Sbjct: 515 IPKGLCKELNGIMARFWWAKAKDKRGIHWVKWELLCKSKFAGGLGFRDLEAFNQALLAKQ 574

BLAST of Lag0001232 vs. TAIR 10
Match: AT4G29090.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 84.3 bits (207), Expect = 2.8e-16
Identity = 53/198 (26.77%), Postives = 86/198 (43.43%), Query Frame = 0

Query: 315 AIPCYTMNCFRLPKKLVQDISTTMARFWWNGDTEDKRIHW-------------------- 374
           A+P YTM CF LPK + + I + +A FWW    E K +HW                    
Sbjct: 2   ALPTYTMACFLLPKTVCKQIISVLADFWWRNKQEAKGMHWKAWDHLSCYKAEGGIGFKDI 61

Query: 375 ----------------------------GRYFPSGDFLDAGVGSRPSYIWRSLVWGRELL 434
                                        RYF   D L+A +GSRPS++W+S+   +E+L
Sbjct: 62  EAFNLALLGKQMWRMLSRPESLMAKVFKSRYFHKSDPLNAPLGSRPSFVWKSIHASQEIL 121

Query: 435 GKGIRWRIGNGERVSVYGSNWI---PDDVCFKV-RSP----VTLGLDSRVTDLLNASGL- 456
            +G R  +GNGE + ++   W+   P     ++ R P     ++    +V+DL++ SG  
Sbjct: 122 RQGARAVVGNGEDIIIWRHKWLDSKPASAALRMQRVPPQEYASVSSILKVSDLIDESGRE 181

BLAST of Lag0001232 vs. TAIR 10
Match: ATMG00310.1 (RNA-directed DNA polymerase (reverse transcriptase)-related family protein )

HSP 1 Score: 84.0 bits (206), Expect = 3.6e-16
Identity = 42/146 (28.77%), Postives = 61/146 (41.78%), Query Frame = 0

Query: 315 AIPCYTMNCFRLPKKLVQDISTTMARFWWNGDTEDKRIHW-------------------- 374
           A+P Y M+CFRL K L + +++ M  FWW+     ++I W                    
Sbjct: 2   ALPVYAMSCFRLSKLLCKKLTSAMTEFWWSSCENKRKISWVAWQKLCKSKEDDGGLGFRD 61

Query: 375 -----------------------------GRYFPSGDFLDAGVGSRPSYIWRSLVWGREL 412
                                         RYFP    ++  VG+RPSY WRS++ GREL
Sbjct: 62  LGWFNQALLAKQSFRIIHQPHTLLSRLLRSRYFPHSSMMECSVGTRPSYAWRSIIHGREL 121

BLAST of Lag0001232 vs. TAIR 10
Match: AT1G43760.1 (DNAse I-like superfamily protein )

HSP 1 Score: 50.4 bits (119), Expect = 4.5e-06
Identity = 32/108 (29.63%), Postives = 47/108 (43.52%), Query Frame = 0

Query: 31  IHP-SVSDEMNSSLVRPFQREEVLRSLNQIHPNKTPGPDGLFGAFYQHSWSVVGVDVVNC 90
           IHP   +D + S L      +E+  ++  +  NK PGPD     F+  SW VV    +  
Sbjct: 514 IHPFRCNDTLASRLSALPSDKEITAAVFAMPRNKAPGPDSFTAEFFWESWFVVKDSTIAA 573

Query: 91  CLNILNNKVSPAPLNEMMIVLIPKKKSPKCITEYRPISLCNVSYKIVS 138
                         N   I LIPK      ++ +RP+S C V YKI++
Sbjct: 574 VKEFFRTGHLLKRFNATAITLIPKVTGVDQLSMFRPVSCCTVVYKIIT 621

BLAST of Lag0001232 vs. TAIR 10
Match: AT3G09510.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 42.7 bits (99), Expect = 9.3e-04
Identity = 21/47 (44.68%), Postives = 27/47 (57.45%), Query Frame = 0

Query: 356 RYFPSGDFLDAGVGSRPSYIWRSLVWGRELLGKGIRWRIGNGERVSV 403
           RYF     LDA V  + SY W SL+ G  LL KG R  IG+G+ + +
Sbjct: 4   RYFKDVSILDAKVRKQQSYGWASLLDGIALLKKGTRHLIGDGQNIRI 50