Lag0001221 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0001221
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Reverse transcriptase domain-containing protein
Location	chr4: 26983002 .. 26983553 (-)
RNA-Seq Expression	Lag0001221
Synteny	Lag0001221

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCGAACTTCAAGCCTATTAGTCTTTGCAACGTAATTTACAAAATAGTAGCTAAGGCCCTGGCGAATAGGATGAAGAGGGTGCTTGACGACATTATATCTCCAACTCAAGCGGCTTTTGTTCCTAAAAGACTCATATTAGATAATGTGATCTTGGGTTTTGAATGTATTCATGCGCTTAATAACAAAAGAAAAGGAAAAGGAGGCCTCATTGCAATGAAACTGGATATGACCAAGGCTTACGACAGAGTTGAATGGGTGTTTTTAAGGAAGATGATGTTGAAGCTGAATTTTGCTGAGGGTTGGACCAGAAAAGTGATTAATTGCATTGAGTCGGTGCAATATGTTGTTCTCATCAATGGAGTTTCCCAGGAAGAGATCTATCCAAAGCGAGGCTTGAGACAGGGAGACCCGTTATCTCCCTATCTCTTTCTTTTATGCGTGGAAGGCTTGTCTGTTATCTCCTTATATCTTTCTTTTATGCGTGGAAGGCTTGTGTACACTTCTAATCAGGAAAGAATTTCTAAACAATTCCAAAGGCTTAAAGATTAG

mRNA sequence

ATGTCGAACTTCAAGCCTATTAGTCTTTGCAACGTAATTTACAAAATAGTAGCTAAGGCCCTGGCGAATAGGATGAAGAGGGTGCTTGACGACATTATATCTCCAACTCAAGCGGCTTTTGTTCCTAAAAGACTCATATTAGATAATGTGATCTTGGGTTTTGAATGTATTCATGCGCTTAATAACAAAAGAAAAGGAAAAGGAGGCCTCATTGCAATGAAACTGGATATGACCAAGGCTTACGACAGAGTTGAATGGGTGTTTTTAAGGAAGATGATGTTGAAGCTGAATTTTGCTGAGGGTTGGACCAGAAAAGTGATTAATTGCATTGAGTCGGTGCAATATGTTGTTCTCATCAATGGAGTTTCCCAGGAAGAGATCTATCCAAAGCGAGGCTTGAGACAGGGAGACCCGTTATCTCCCTATCTCTTTCTTTTATGCGTGGAAGGCTTGTCTGTTATCTCCTTATATCTTTCTTTTATGCGTGGAAGGCTTGTGTACACTTCTAATCAGGAAAGAATTTCTAAACAATTCCAAAGGCTTAAAGATTAG

Coding sequence (CDS)

ATGTCGAACTTCAAGCCTATTAGTCTTTGCAACGTAATTTACAAAATAGTAGCTAAGGCCCTGGCGAATAGGATGAAGAGGGTGCTTGACGACATTATATCTCCAACTCAAGCGGCTTTTGTTCCTAAAAGACTCATATTAGATAATGTGATCTTGGGTTTTGAATGTATTCATGCGCTTAATAACAAAAGAAAAGGAAAAGGAGGCCTCATTGCAATGAAACTGGATATGACCAAGGCTTACGACAGAGTTGAATGGGTGTTTTTAAGGAAGATGATGTTGAAGCTGAATTTTGCTGAGGGTTGGACCAGAAAAGTGATTAATTGCATTGAGTCGGTGCAATATGTTGTTCTCATCAATGGAGTTTCCCAGGAAGAGATCTATCCAAAGCGAGGCTTGAGACAGGGAGACCCGTTATCTCCCTATCTCTTTCTTTTATGCGTGGAAGGCTTGTCTGTTATCTCCTTATATCTTTCTTTTATGCGTGGAAGGCTTGTGTACACTTCTAATCAGGAAAGAATTTCTAAACAATTCCAAAGGCTTAAAGATTAG

Protein sequence

MSNFKPISLCNVIYKIVAKALANRMKRVLDDIISPTQAAFVPKRLILDNVILGFECIHALNNKRKGKGGLIAMKLDMTKAYDRVEWVFLRKMMLKLNFAEGWTRKVINCIESVQYVVLINGVSQEEIYPKRGLRQGDPLSPYLFLLCVEGLSVISLYLSFMRGRLVYTSNQERISKQFQRLKD

Homology

[Close]

BLAST of Lag0001221 vs. NCBI nr
Match: XP_035539661.1 (uncharacterized protein LOC118344031 [Juglans regia])

HSP 1 Score: 206.1 bits (523), Expect = 2.6e-49
Identity = 95/149 (63.76%), Postives = 117/149 (78.52%), Query Frame = 0


Query: 4   FKPISLCNVIYKIVAKALANRMKRVLDDIISPTQAAFVPKRLILDNVILGFECIHALNNK 63
           F+PISLCNVIYKI++KA+ANR+K+VL  IISP+Q+AFVP RLI DNVI+ FE  H +N K
Sbjct: 118 FRPISLCNVIYKIISKAIANRLKKVLHGIISPSQSAFVPNRLITDNVIVAFEAFHTMNCK 177

Query: 64  RKGKGGLIAMKLDMTKAYDRVEWVFLRKMMLKLNFAEGWTRKVINCIESVQYVVLINGVS 123
             GK G +A+KLDM+KAYDRVEW FLR ++  L F+  W   V+ CIE+V Y +++NGV 
Sbjct: 178 MTGKEGYMALKLDMSKAYDRVEWSFLRVVLYNLGFSSQWVELVMKCIETVSYALMVNGVP 237

Query: 124 QEEIYPKRGLRQGDPLSPYLFLLCVEGLS 153
           Q E YP RGLRQGDPLSPYLF+LC E LS
Sbjct: 238 QPEFYPTRGLRQGDPLSPYLFILCAEALS 266

[Close]

BLAST of Lag0001221 vs. NCBI nr
Match: XP_039834390.1 (uncharacterized protein LOC120695147 [Panicum virgatum])

HSP 1 Score: 204.9 bits (520), Expect = 5.9e-49
Identity = 93/154 (60.39%), Postives = 121/154 (78.57%), Query Frame = 0


Query: 1   MSNFKPISLCNVIYKIVAKALANRMKRVLDDIISPTQAAFVPKRLILDNVILGFECIHAL 60
           + + +PISLCNV+YK+++K LANR+K VL +IISP+Q+AFVP RLI DNV+L +E  H L
Sbjct: 371 LKDLRPISLCNVVYKLISKVLANRLKVVLPEIISPSQSAFVPGRLITDNVLLAYELTHYL 430

Query: 61  NNKRKGKGGLIAMKLDMTKAYDRVEWVFLRKMMLKLNFAEGWTRKVINCIESVQYVVLIN 120
           N +R GK G+ A+KLDM+KAYDRVEW FL KMMLKL FA  W  +V+ C+ +V Y + +N
Sbjct: 431 NQRRWGKNGVAAIKLDMSKAYDRVEWCFLEKMMLKLGFAVQWVERVMKCVSTVSYHIKVN 490

Query: 121 GVSQEEIYPKRGLRQGDPLSPYLFLLCVEGLSVI 155
           G    +IYP+RGLRQG+PLSPYLF+LC EGLS +
Sbjct: 491 GDHSNKIYPQRGLRQGNPLSPYLFILCAEGLSAL 524

[Close]

BLAST of Lag0001221 vs. NCBI nr
Match: KAF5447348.1 (hypothetical protein F2P56_032905 [Juglans regia])

HSP 1 Score: 201.8 bits (512), Expect = 5.0e-48
Identity = 90/152 (59.21%), Postives = 125/152 (82.24%), Query Frame = 0


Query: 1   MSNFKPISLCNVIYKIVAKALANRMKRVLDDIISPTQAAFVPKRLILDNVILGFECIHAL 60
           +S+++PISLCNVIYK+V+KA+ANR+K +L DIIS TQ+AFVP RLI DN+I+ FE +H +
Sbjct: 426 VSDYRPISLCNVIYKMVSKAIANRLKSILPDIISSTQSAFVPGRLISDNLIVAFEALHTM 485

Query: 61  NNKRKGKGGLIAMKLDMTKAYDRVEWVFLRKMMLKLNFAEGWTRKVINCIESVQYVVLIN 120
           +++  GK GL+A+KLDM+KAYDR+EW FL+++MLK+ FA  W + V+NC+E+V Y +++N
Sbjct: 486 HSRLGGKEGLMALKLDMSKAYDRIEWQFLKEVMLKMGFATEWVQLVMNCVETVSYSIILN 545

Query: 121 GVSQEEIYPKRGLRQGDPLSPYLFLLCVEGLS 153
           G  QE   P RG+RQGDPLSPYLF+LC E L+
Sbjct: 546 GSPQEVFIPTRGIRQGDPLSPYLFILCAEVLT 577

[Close]

BLAST of Lag0001221 vs. NCBI nr
Match: XP_042972818.1 (uncharacterized protein LOC122304624 [Carya illinoinensis])

HSP 1 Score: 201.8 bits (512), Expect = 5.0e-48
Identity = 87/152 (57.24%), Postives = 125/152 (82.24%), Query Frame = 0


Query: 1   MSNFKPISLCNVIYKIVAKALANRMKRVLDDIISPTQAAFVPKRLILDNVILGFECIHAL 60
           ++ ++PISLCNV+YKIV+KA++NR+K++L  IISPTQ+AF+P RLI DN+++ FE +H++
Sbjct: 46  VNEYRPISLCNVLYKIVSKAISNRLKKILASIISPTQSAFLPSRLISDNIMVAFELLHSM 105

Query: 61  NNKRKGKGGLIAMKLDMTKAYDRVEWVFLRKMMLKLNFAEGWTRKVINCIESVQYVVLIN 120
             +++GK G +A+KLDM+KAYDRVEW FL  +M KL F E W + ++ C++SV Y VL+N
Sbjct: 106 KTRKRGKVGSMAIKLDMSKAYDRVEWDFLEVVMKKLGFNEKWVKLIMKCVKSVSYSVLVN 165

Query: 121 GVSQEEIYPKRGLRQGDPLSPYLFLLCVEGLS 153
           G+  ++ +PKRGLRQGDPLSPYLF++C EGLS
Sbjct: 166 GIPGKKFWPKRGLRQGDPLSPYLFIMCAEGLS 197

[Close]

BLAST of Lag0001221 vs. NCBI nr
Match: XP_042952220.1 (uncharacterized protein LOC122289300 [Carya illinoinensis])

HSP 1 Score: 199.9 bits (507), Expect = 1.9e-47
Identity = 98/182 (53.85%), Postives = 129/182 (70.88%), Query Frame = 0


Query: 1   MSNFKPISLCNVIYKIVAKALANRMKRVLDDIISPTQAAFVPKRLILDNVILGFECIHAL 60
           ++ F PISLCNV YK+++K +ANR+K+VL  IISP+Q+AFVP RLI DN+I+ FE +H +
Sbjct: 450 VTEFIPISLCNVTYKLISKVIANRLKKVLPSIISPSQSAFVPNRLISDNIIVAFETLHTM 509

Query: 61  NNKRKGKGGLIAMKLDMTKAYDRVEWVFLRKMMLKLNFAEGWTRKVINCIESVQYVVLIN 120
             K  GK G +A+KLDM+KAYDRVEW FLR ++ KL F++ W   V+NCIE+V Y +L+N
Sbjct: 510 KCKMAGKEGYMALKLDMSKAYDRVEWCFLRAVLYKLGFSKTWVELVMNCIETVSYAILVN 569

Query: 121 GVSQEEIYPKRGLRQGDPLSPYLFLLCVEGLSVISLYLSFMRGRLVY----TSNQERISK 179
           G  Q    P RG+RQGDPLSPYLF+LC E LS  +L      GRL++      +Q RIS 
Sbjct: 570 GEPQATFQPSRGIRQGDPLSPYLFILCAEALS--NLIQKAEEGRLIHGVPVARSQMRISH 629

[Close]

BLAST of Lag0001221 vs. ExPASy Swiss-Prot
Match: P11369 (LINE-1 retrotransposable element ORF2 protein OS=Mus musculus OX=10090 GN=Pol PE=1 SV=2)

HSP 1 Score: 84.3 bits (207), Expect = 1.5e-15
Identity = 58/153 (37.91%), Postives = 85/153 (55.56%), Query Frame = 0


Query: 1   MSNFKPISLCNVIYKIVAKALANRMKRVLDDIISPTQAAFVPKRLILDNVILGFECIHAL 60
           + NF+PISL N+  KI+ K LANR++  +  II P Q  F+P      N+      IH +
Sbjct: 534 IENFRPISLMNIDAKILNKILANRIQEHIKAIIHPDQVGFIPGMQGWFNIRKSINVIHYI 593

Query: 61  NNKRKGKGGLIAMKLDMTKAYDRVEWVFLRKMMLKLNFAEGWTRKVINCIESVQYV-VLI 120
            NK K K  +I + LD  KA+D+++  F+ K +L+ +  +G    +I  I S     + +
Sbjct: 594 -NKLKDKNHMI-ISLDAEKAFDKIQHPFMIK-VLERSGIQGPYLNMIKAIYSKPVANIKV 653

Query: 121 NGVSQEEIYPKRGLRQGDPLSPYLFLLCVEGLS 153
           NG   E I  K G RQG PLSPYLF + +E L+
Sbjct: 654 NGEKLEAIPLKSGTRQGCPLSPYLFNIVLEVLA 683

[Close]

BLAST of Lag0001221 vs. ExPASy Swiss-Prot
Match: P14381 (Transposon TX1 uncharacterized 149 kDa protein OS=Xenopus laevis OX=8355 PE=4 SV=1)

HSP 1 Score: 83.6 bits (205), Expect = 2.6e-15
Identity = 57/165 (34.55%), Postives = 87/165 (52.73%), Query Frame = 0


Query: 1   MSNFKPISLCNVIYKIVAKALANRMKRVLDDIISPTQAAFVPKRLILDNVILGFECIHAL 60
           + N++P+SL +  YKIVAKA++ R+K VL ++I P Q+  VP R I DNV L  + +H  
Sbjct: 522 IKNWRPVSLLSTDYKIVAKAISLRLKSVLAEVIHPDQSYTVPGRTIFDNVFLIRDLLHF- 581

Query: 61  NNKRKGKGGLIAMKLDMTKAYDRVEWVFLRKMMLKLNFAEGWTRKVINCIESVQYVVLIN 120
              R+    L  + LD  KA+DRV+  +L   +   +F   +   +     S + +V IN
Sbjct: 582 --ARRTGLSLAFLSLDQEKAFDRVDHQYLIGTLQAYSFGPQFVGYLKTMYASAECLVKIN 641

Query: 121 GVSQEEIYPKRGLRQGDPLSPYLFLLCVEGLSVISLYLSFMRGRL 166
                 +   RG+RQG PLS  L+ L +E       +L  +R RL
Sbjct: 642 WSLTAPLAFGRGVRQGCPLSGQLYSLAIEP------FLCLLRKRL 677

[Close]

BLAST of Lag0001221 vs. ExPASy Swiss-Prot
Match: O00370 (LINE-1 retrotransposable element ORF2 protein OS=Homo sapiens OX=9606 PE=1 SV=1)

HSP 1 Score: 76.6 bits (187), Expect = 3.2e-13
Identity = 52/151 (34.44%), Postives = 82/151 (54.30%), Query Frame = 0


Query: 3   NFKPISLCNVIYKIVAKALANRMKRVLDDIISPTQAAFVPKRLILDNVILGFECIHALNN 62
           NF+PISL N+  KI+ K LANR+++ +  +I   Q  F+P      N+      I  + N
Sbjct: 529 NFRPISLMNIDAKILNKILANRIQQHIKKLIHHDQVGFIPGMQGWFNIRKSINVIQHI-N 588

Query: 63  KRKGKGGLIAMKLDMTKAYDRVEWVFLRKMMLKLNFAEGWTRKVINCI-ESVQYVVLING 122
           + K K  +I + +D  KA+D+++  F+ K + KL   +G   K+I  I +     +++NG
Sbjct: 589 RAKDKNHVI-ISIDAEKAFDKIQQPFMLKTLNKLGI-DGMYLKIIRAIYDKPTANIILNG 648

Query: 123 VSQEEIYPKRGLRQGDPLSPYLFLLCVEGLS 153
              E    K G RQG PLSP LF + +E L+
Sbjct: 649 QKLEAFPLKTGTRQGCPLSPLLFNIVLEVLA 676

[Close]

BLAST of Lag0001221 vs. ExPASy Swiss-Prot
Match: P08548 (LINE-1 reverse transcriptase homolog OS=Nycticebus coucang OX=9470 PE=4 SV=1)

HSP 1 Score: 75.9 bits (185), Expect = 5.4e-13
Identity = 51/152 (33.55%), Postives = 82/152 (53.95%), Query Frame = 0


Query: 3   NFKPISLCNVIYKIVAKALANRMKRVLDDIISPTQAAFVPKRLILDNVILGFECIHALNN 62
           N++PISL N+  KI+ K L NR+++ +  II   Q  F+P      N+      I  + N
Sbjct: 528 NYRPISLMNIDAKILNKILTNRIQQHIKKIIHHDQVGFIPGSQGWFNIRKSINVIQHI-N 587

Query: 63  KRKGKGGLIAMKLDMTKAYDRVEWVFLRKMMLKLNFAEGWTRKVINCIESVQYV-VLING 122
           K K K  +I + +D  KA+D ++  F+ + + K+   EG   K+I  I S     +++NG
Sbjct: 588 KLKNKDHMI-LSIDAEKAFDNIQHPFMIRTLKKIGI-EGTFLKLIEAIYSKPTANIILNG 647

Query: 123 VSQEEIYPKRGLRQGDPLSPYLFLLCVEGLSV 154
           V  +    + G RQG PLSP LF + +E L++
Sbjct: 648 VKLKSFPLRSGTRQGCPLSPLLFNIVMEVLAI 676

[Close]

BLAST of Lag0001221 vs. ExPASy Swiss-Prot
Match: P16423 (Retrovirus-related Pol polyprotein from type-2 retrotransposable element R2DM OS=Drosophila melanogaster OX=7227 GN=pol PE=4 SV=1)

HSP 1 Score: 58.5 bits (140), Expect = 8.9e-08
Identity = 43/149 (28.86%), Postives = 70/149 (46.98%), Query Frame = 0


Query: 3   NFKPISLCNVIYKIVAKALANRMKRVLDDIISPTQAAFVPKRLILDNVILGFECIHALNN 62
           +F+PIS+ +V+ + +   LA R+   ++    P Q  F+P     DN  +       L +
Sbjct: 414 DFRPISVPSVLVRQLNAILATRLNSSIN--WDPRQRGFLPTDGCADNATI---VDLVLRH 473

Query: 63  KRKGKGGLIAMKLDMTKAYDRVEWVFLRKMMLKLNFAEGWTRKVINCIESVQYVVLINGV 122
             K         LD++KA+D +    +   +      +G+   V N  E     +  +G 
Sbjct: 474 SHKHFRSCYIANLDVSKAFDSLSHASIYDTLRAYGAPKGFVDYVQNTYEGGGTSLNGDGW 533

Query: 123 SQEEIYPKRGLRQGDPLSPYLFLLCVEGL 152
           S EE  P RG++QGDPLSP LF L ++ L
Sbjct: 534 SSEEFVPARGVKQGDPLSPILFNLVMDRL 557

The following BLAST results are available for this feature:

BLAST of Lag0001221 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_035539661.1	2.6e-49	63.76	uncharacterized protein LOC118344031 [Juglans regia]	[more]
XP_039834390.1	5.9e-49	60.39	uncharacterized protein LOC120695147 [Panicum virgatum]	[more]
KAF5447348.1	5.0e-48	59.21	hypothetical protein F2P56_032905 [Juglans regia]	[more]
XP_042972818.1	5.0e-48	57.24	uncharacterized protein LOC122304624 [Carya illinoinensis]	[more]
XP_042952220.1	1.9e-47	53.85	uncharacterized protein LOC122289300 [Carya illinoinensis]	[more]

BLAST of Lag0001221 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P11369	1.5e-15	37.91	LINE-1 retrotransposable element ORF2 protein OS=Mus musculus OX=10090 GN=Pol PE...	[more]
P14381	2.6e-15	34.55	Transposon TX1 uncharacterized 149 kDa protein OS=Xenopus laevis OX=8355 PE=4 SV...	[more]
O00370	3.2e-13	34.44	LINE-1 retrotransposable element ORF2 protein OS=Homo sapiens OX=9606 PE=1 SV=1	[more]
P08548	5.4e-13	33.55	LINE-1 reverse transcriptase homolog OS=Nycticebus coucang OX=9470 PE=4 SV=1	[more]
P16423	8.9e-08	28.86	Retrovirus-related Pol polyprotein from type-2 retrotransposable element R2DM OS...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000477	Reverse transcriptase domain	PFAM	PF00078	RVT_1	coord: 2..151 e-value: 5.8E-28 score: 97.9
IPR000477	Reverse transcriptase domain	PROSITE	PS50878	RT_POL	coord: 1..183 score: 9.757074
None	No IPR available	PANTHER	PTHR19446	REVERSE TRANSCRIPTASES	coord: 2..180
None	No IPR available	PANTHER	PTHR19446:SF440	SUBFAMILY NOT NAMED	coord: 2..180
None	No IPR available	CDD	cd01650	RT_nLTR_like	coord: 1..152 e-value: 1.16712E-36 score: 124.711
IPR043502	DNA/RNA polymerase superfamily	SUPERFAMILY	56672	DNA/RNA polymerases	coord: 6..152

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0001221.1	Lag0001221.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0090502	RNA phosphodiester bond hydrolysis, endonucleolytic
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0004523	RNA-DNA hybrid ribonuclease activity