Lag0001200 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0001200
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	DNA polymerase epsilon subunit
Location	chr4: 26488571 .. 26489023 (+)
RNA-Seq Expression	Lag0001200
Synteny	Lag0001200

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATGCAACCACTCTAAGGAGAATGGTACAGAAAAAATCAAAATTCAGAGGTTACCACATCAAACTAGATGCTCTCAATGAGATTGTTTCCTTTGCCTCTAGCTTCCCTGGCTGCGAAGACGATGCTATAGATCTTGTTCTCGACAACCTCCATGACGAATCTTGTACTACTATATCTCATTTTTATTTCATAATGCAATTATATCATTTCTCTACAATTCATTGCATTCCTTCTCATGGACAATTTCTTCGTTTTTTGGTCGTGGCAGTGCAATCTTCAATATTAGACGAAGACGCAGTGCATCGGGTTGTTAGCAGTTTACGCTCAGCTGCTGGAGTGGGAGAAGAAAGTCCTAATACCATTACTAGTACATCTGCTCTTTGCATCATAGACTCTTTTGATGTCTCCAAGTTTCATTACGATCCCATCAAGAAGATCTTCCACCTGTAA

mRNA sequence

ATGGATGCAACCACTCTAAGGAGAATGGTACAGAAAAAATCAAAATTCAGAGGTTACCACATCAAACTAGATGCTCTCAATGAGATTGTTTCCTTTGCCTCTAGCTTCCCTGGCTGCGAAGACGATGCTATAGATCTTGTTCTCGACAACCTCCATGACGAATCTTTGCAATCTTCAATATTAGACGAAGACGCAGTGCATCGGGTTGTTAGCAGTTTACGCTCAGCTGCTGGAGTGGGAGAAGAAAGTCCTAATACCATTACTAGTACATCTGCTCTTTGCATCATAGACTCTTTTGATGTCTCCAAGTTTCATTACGATCCCATCAAGAAGATCTTCCACCTGTAA

Coding sequence (CDS)

ATGGATGCAACCACTCTAAGGAGAATGGTACAGAAAAAATCAAAATTCAGAGGTTACCACATCAAACTAGATGCTCTCAATGAGATTGTTTCCTTTGCCTCTAGCTTCCCTGGCTGCGAAGACGATGCTATAGATCTTGTTCTCGACAACCTCCATGACGAATCTTTGCAATCTTCAATATTAGACGAAGACGCAGTGCATCGGGTTGTTAGCAGTTTACGCTCAGCTGCTGGAGTGGGAGAAGAAAGTCCTAATACCATTACTAGTACATCTGCTCTTTGCATCATAGACTCTTTTGATGTCTCCAAGTTTCATTACGATCCCATCAAGAAGATCTTCCACCTGTAA

Protein sequence

MDATTLRRMVQKKSKFRGYHIKLDALNEIVSFASSFPGCEDDAIDLVLDNLHDESLQSSILDEDAVHRVVSSLRSAAGVGEESPNTITSTSALCIIDSFDVSKFHYDPIKKIFHL

Homology

[Close]

BLAST of Lag0001200 vs. NCBI nr
Match: XP_038894268.1 (DNA polymerase epsilon subunit B [Benincasa hispida])

HSP 1 Score: 175.3 bits (443), Expect = 3.1e-40
Identity = 91/116 (78.45%), Postives = 103/116 (88.79%), Query Frame = 0


Query: 1   MDATTLRRMVQKKSKFRG-YHIKLDALNEIVSFASSFPGCEDDAIDLVLDNLHDESLQSS 60
           MDA TLR+ VQ+K+K RG Y IKL+AL+EIVSF S F GCEDDAIDLVLDNLHDESL+SS
Sbjct: 1   MDALTLRKKVQRKAKIRGLYSIKLEALDEIVSFVSRFHGCEDDAIDLVLDNLHDESLKSS 60

Query: 61  ILDEDAVHRVVSSLRSAAGVGEESPNTITSTSALCIIDSFDVSKFHYDPIKKIFHL 116
           ILD+DAVHRVVS + +A  VGEESPNTITSTSALCIID+FD+SKF YDPIKKIF+L
Sbjct: 61  ILDKDAVHRVVSIMLAADEVGEESPNTITSTSALCIIDAFDISKFRYDPIKKIFYL 116

[Close]

BLAST of Lag0001200 vs. NCBI nr
Match: XP_023525784.1 (DNA polymerase epsilon subunit B [Cucurbita pepo subsp. pepo])

HSP 1 Score: 174.5 bits (441), Expect = 5.3e-40
Identity = 89/115 (77.39%), Postives = 100/115 (86.96%), Query Frame = 0


Query: 1   MDATTLRRMVQKKSKFRGYHIKLDALNEIVSFASSFPGCEDDAIDLVLDNLHDESLQSSI 60
           MD +TLRR VQ+KSK RGY IKLDAL EIVSFAS F GCEDDAIDLVLD+LHDESLQSSI
Sbjct: 1   MDESTLRRKVQRKSKIRGYSIKLDALAEIVSFASRFDGCEDDAIDLVLDHLHDESLQSSI 60

Query: 61  LDEDAVHRVVSSLRSAAGVGEESPNTITSTSALCIIDSFDVSKFHYDPIKKIFHL 116
           +D+DAVHRVVS L +A   GEE P+T TSTSALCIID+FD+SKF YDPIKKIF++
Sbjct: 61  IDKDAVHRVVSILLAADEAGEECPDTSTSTSALCIIDAFDISKFRYDPIKKIFYM 115

[Close]

BLAST of Lag0001200 vs. NCBI nr
Match: KAG6607399.1 (DNA polymerase epsilon subunit B, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 173.3 bits (438), Expect = 1.2e-39
Identity = 88/115 (76.52%), Postives = 100/115 (86.96%), Query Frame = 0


Query: 1   MDATTLRRMVQKKSKFRGYHIKLDALNEIVSFASSFPGCEDDAIDLVLDNLHDESLQSSI 60
           MD +TLRR VQ+KSK RGY IKLDAL EIVSFAS F GCEDDAIDLVLD+LHDESLQSSI
Sbjct: 1   MDESTLRRKVQRKSKIRGYSIKLDALAEIVSFASRFDGCEDDAIDLVLDHLHDESLQSSI 60

Query: 61  LDEDAVHRVVSSLRSAAGVGEESPNTITSTSALCIIDSFDVSKFHYDPIKKIFHL 116
           +D+DAVHRVVS L +A   GEE P+T TSTSALCIID+FD+SK+ YDPIKKIF++
Sbjct: 61  IDKDAVHRVVSILLAADEAGEECPDTSTSTSALCIIDAFDISKYRYDPIKKIFYM 115

[Close]

BLAST of Lag0001200 vs. NCBI nr
Match: XP_022949454.1 (DNA polymerase epsilon subunit B [Cucurbita moschata])

HSP 1 Score: 173.3 bits (438), Expect = 1.2e-39
Identity = 88/115 (76.52%), Postives = 100/115 (86.96%), Query Frame = 0


Query: 1   MDATTLRRMVQKKSKFRGYHIKLDALNEIVSFASSFPGCEDDAIDLVLDNLHDESLQSSI 60
           MD +TLRR VQ+KSK RGY IKLDAL EIVSFAS F GCEDDAIDLVLD+LHDESLQSSI
Sbjct: 1   MDESTLRRKVQRKSKIRGYSIKLDALAEIVSFASRFDGCEDDAIDLVLDHLHDESLQSSI 60

Query: 61  LDEDAVHRVVSSLRSAAGVGEESPNTITSTSALCIIDSFDVSKFHYDPIKKIFHL 116
           +D+DAVHRVVS L +A   GEE P+T TSTSALCIID+FD+SK+ YDPIKKIF++
Sbjct: 61  IDKDAVHRVVSILLAADEAGEECPDTSTSTSALCIIDAFDISKYRYDPIKKIFYM 115

[Close]

BLAST of Lag0001200 vs. NCBI nr
Match: KAG6607402.1 (DNA polymerase epsilon subunit B, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 173.3 bits (438), Expect = 1.2e-39
Identity = 88/115 (76.52%), Postives = 100/115 (86.96%), Query Frame = 0


Query: 1   MDATTLRRMVQKKSKFRGYHIKLDALNEIVSFASSFPGCEDDAIDLVLDNLHDESLQSSI 60
           MD +TLRR VQ+KSK RGY IKLDAL EIVSFAS F GCEDDAIDLVLD+LHDESLQSSI
Sbjct: 1   MDESTLRRKVQRKSKIRGYSIKLDALAEIVSFASRFDGCEDDAIDLVLDHLHDESLQSSI 60

Query: 61  LDEDAVHRVVSSLRSAAGVGEESPNTITSTSALCIIDSFDVSKFHYDPIKKIFHL 116
           +D+DAVHRVVS L +A   GEE P+T TSTSALCIID+FD+SK+ YDPIKKIF++
Sbjct: 61  IDKDAVHRVVSILLAADEAGEECPDTSTSTSALCIIDAFDISKYRYDPIKKIFYM 115

[Close]

BLAST of Lag0001200 vs. ExPASy Swiss-Prot
Match: Q500V9 (DNA polymerase epsilon subunit B OS=Arabidopsis thaliana OX=3702 GN=DPB2 PE=1 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 7.3e-16
Identity = 52/109 (47.71%), Postives = 73/109 (66.97%), Query Frame = 0


Query: 7   RRMVQKKSKFRGYHIKLDALNEIVSFASSFPGCED-DAIDLVLDNLHDESLQSSILDEDA 66
           R+ +QKK K RGY++K DAL+EI+ FA  FP  +D +AIDL+LDNL  E+ +SS +D ++
Sbjct: 8   RKKIQKKFKNRGYNLKFDALDEILVFADQFPDDDDGEAIDLLLDNL-QETHKSSTVDAES 67

Query: 67  VHRVVSSLRSAAGVGEESPNTITSTSALCIIDSFDVSKFHYDPIKKIFH 115
           V  +++ L  A    EE     TS S+L IID+F V KF YD +KK F+
Sbjct: 68  VRGLINRLLGAHNAPEE---PTTSASSLAIIDAFLVPKFGYDSVKKKFN 112

The following BLAST results are available for this feature:

BLAST of Lag0001200 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038894268.1	3.1e-40	78.45	DNA polymerase epsilon subunit B [Benincasa hispida]	[more]
XP_023525784.1	5.3e-40	77.39	DNA polymerase epsilon subunit B [Cucurbita pepo subsp. pepo]	[more]
KAG6607399.1	1.2e-39	76.52	DNA polymerase epsilon subunit B, partial [Cucurbita argyrosperma subsp. sororia...	[more]
XP_022949454.1	1.2e-39	76.52	DNA polymerase epsilon subunit B [Cucurbita moschata]	[more]
KAG6607402.1	1.2e-39	76.52	DNA polymerase epsilon subunit B, partial [Cucurbita argyrosperma subsp. sororia...	[more]

BLAST of Lag0001200 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q500V9	7.3e-16	47.71	DNA polymerase epsilon subunit B OS=Arabidopsis thaliana OX=3702 GN=DPB2 PE=1 SV...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01

Position :

Zoom :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	1.10.8.60		coord: 1..72 e-value: 3.4E-10 score: 41.9
IPR016266	DNA polymerase epsilon, subunit B	PANTHER	PTHR12708	DNA POLYMERASE EPSILON SUBUNIT B	coord: 7..114

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0001200.1	Lag0001200.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006261	DNA-dependent DNA replication
biological_process	GO:0009793	embryo development ending in seed dormancy
biological_process	GO:0042276	error-prone translesion synthesis
biological_process	GO:0006536	glutamate metabolic process
biological_process	GO:0051781	positive regulation of cell division
cellular_component	GO:0008622	epsilon DNA polymerase complex
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0070182	DNA polymerase binding
molecular_function	GO:0004351	glutamate decarboxylase activity
molecular_function	GO:0030170	pyridoxal phosphate binding