Lag0001159 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0001159
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Retrovirus-related Pol polyprotein from transposon TNT 1-94
Location	chr4: 25766021 .. 25767233 (+)
RNA-Seq Expression	Lag0001159
Synteny	Lag0001159

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTGTTTGTCGATGGATGTGGCAAGTCTAGTAGCCCATGAGACAACTGCGGTCAAGTTGATGGAATCGCTTACAAACAGGTATGAAAAACCCTCTGCAAATAATAAGGTCTACGTAGTTAAGAAGTTTTTCAACATGCAAATGTCTGAGGAGGCTTCTGTGAATTCCTATATTAATGAGGTTACCACTTTGATTAATCAGTTAAAATCTGTTAAGATAGAATTTTCTGATGAGGTGTATGCTATTCAGTTGTTAACGTCTTTACTTGATAGTTGGGAAACGATGAAGACACCAGTGTCTAATTCGACTGGAAATAACACTTTAAAATTTTCAGAAGTTTGTGATTTAGCCATAGCTGAGGAAATTCGTAGGCAGGGTAGTAATAAAGAGTCTACTGTAGGGTCAGCTTTGGTTATGACTAAAGGTAAAGATAAGGTTGATGAAGATAATGAACCGAGTAGCAGTAGGAAAAAGTGGAAAGGTAGGAATGAGGTAGAATGTTATTACTGCCATAAGAAAGGTCACTTCAAGTATCAGTGTAGGAAATTTAAAGAGGATCAGAAAAGAAAGCCAGAGGCAAATATAGTGGAAGAGGTTGTCTTAGCTTGTGTTGAGAGTGACACAAAGTATAGTAACCACTCATCAGGTTGGATATTAGACAGTGCAGCTTCTGTTCACATAGCTTCAGATAGGAGTTTGTTCACATCATTCACAGGAGGGCATCATGGCCTAGTGAGGATGGGGAATGGTAGAACCTCCAAGACTAGAGGGATTGGAGATGTTAGTCTGAAGACAGAATGTGGAGGTAAATTGGTACTGCGAGATGTCAGGTACGTGCCTAATATCAAGATGAATCTTATTTCTATTGGTAAGTTGGCAGATGATGGTTACATGTGTGAGTTTGGTAGTCGCCTACCAGCGCGTTAGAGCGACTACCAGCACGTCGTTGTGTGGATGACGTATTCCCGCAGCCTCCTTCTCTGTAAACTCTAACTTTGGTGAGTTTTCATCAAGTAGGAGCGAGTACATACCTTGCCCTCCGGGCTCTTGCTGTGCCTCTCGAACTGCAGCTTTTCGCTTTCTACCTGACTCTCCTCCTGAGGTTCCTCCTAAAATTGTTCGAATCTCGAGCGGTGGGTTGGCACCACCCTTACCTTGGTCTGCTGGTAGTGGCCTCTTGCTTCTGTCATTCCCCATGAATTCATTGAGGTAA

mRNA sequence

ATGTGTTTGTCGATGGATGTGGCAAGTCTAGTAGCCCATGAGACAACTGCGGTCAAGTTGATGGAATCGCTTACAAACAGGTATGAAAAACCCTCTGCAAATAATAAGGTCTACGTAGTTAAGAAGTTTTTCAACATGCAAATGTCTGAGGAGGCTTCTGTGAATTCCTATATTAATGAGGTTACCACTTTGATTAATCAGTTAAAATCTGTTAAGATAGAATTTTCTGATGAGGTGTATGCTATTCAGTTGTTAACGTCTTTACTTGATAGTTGGGAAACGATGAAGACACCAGTGTCTAATTCGACTGGAAATAACACTTTAAAATTTTCAGAAGTTTGTGATTTAGCCATAGCTGAGGAAATTCGTAGGCAGGGTAGTAATAAAGAGTCTACTGTAGGGTCAGCTTTGGTTATGACTAAAGGTAAAGATAAGGTTGATGAAGATAATGAACCGAGTAGCAGTAGGAAAAAGTGGAAAGGTAGGAATGAGGTAGAATGTTATTACTGCCATAAGAAAGGTCACTTCAAGTATCAGTGTAGGAAATTTAAAGAGGATCAGAAAAGAAAGCCAGAGGCAAATATAGTGGAAGAGGTTGTCTTAGCTTGTGTTGAGAGTGACACAAAGTATAGTAACCACTCATCAGGTTGGATATTAGACAGTGCAGCTTCTGTTCACATAGCTTCAGATAGGAGTTTGTTCACATCATTCACAGGAGGGCATCATGGCCTAGTGAGGATGGGGAATGGTAGAACCTCCAAGACTAGAGGGATTGGAGATGTTAGTCTGAAGACAGAATGTGGAGGTAAATTGGTACTGCGAGATGTCAGGTACGTGCCTAATATCAAGATGAATCTTATTTCTATTGGTAAGTTGGCAGATGATGGTTACATGTCCTCCTTCTCTGTAAACTCTAACTTTGGTGAGTTTTCATCAAGTAGGAGCGAGTACATACCTTGCCCTCCGGGCTCTTGCTGTGCCTCTCGAACTGCAGCTTTTCGCTTTCTACCTGACTCTCCTCCTGAGGTTCCTCCTAAAATTGTTCGAATCTCGAGCGGTGGGTTGGCACCACCCTTACCTTGGTCTGCTGGTAGTGGCCTCTTGCTTCTGTCATTCCCCATGAATTCATTGAGGTAA

Coding sequence (CDS)

ATGTGTTTGTCGATGGATGTGGCAAGTCTAGTAGCCCATGAGACAACTGCGGTCAAGTTGATGGAATCGCTTACAAACAGGTATGAAAAACCCTCTGCAAATAATAAGGTCTACGTAGTTAAGAAGTTTTTCAACATGCAAATGTCTGAGGAGGCTTCTGTGAATTCCTATATTAATGAGGTTACCACTTTGATTAATCAGTTAAAATCTGTTAAGATAGAATTTTCTGATGAGGTGTATGCTATTCAGTTGTTAACGTCTTTACTTGATAGTTGGGAAACGATGAAGACACCAGTGTCTAATTCGACTGGAAATAACACTTTAAAATTTTCAGAAGTTTGTGATTTAGCCATAGCTGAGGAAATTCGTAGGCAGGGTAGTAATAAAGAGTCTACTGTAGGGTCAGCTTTGGTTATGACTAAAGGTAAAGATAAGGTTGATGAAGATAATGAACCGAGTAGCAGTAGGAAAAAGTGGAAAGGTAGGAATGAGGTAGAATGTTATTACTGCCATAAGAAAGGTCACTTCAAGTATCAGTGTAGGAAATTTAAAGAGGATCAGAAAAGAAAGCCAGAGGCAAATATAGTGGAAGAGGTTGTCTTAGCTTGTGTTGAGAGTGACACAAAGTATAGTAACCACTCATCAGGTTGGATATTAGACAGTGCAGCTTCTGTTCACATAGCTTCAGATAGGAGTTTGTTCACATCATTCACAGGAGGGCATCATGGCCTAGTGAGGATGGGGAATGGTAGAACCTCCAAGACTAGAGGGATTGGAGATGTTAGTCTGAAGACAGAATGTGGAGGTAAATTGGTACTGCGAGATGTCAGGTACGTGCCTAATATCAAGATGAATCTTATTTCTATTGGTAAGTTGGCAGATGATGGTTACATGTCCTCCTTCTCTGTAAACTCTAACTTTGGTGAGTTTTCATCAAGTAGGAGCGAGTACATACCTTGCCCTCCGGGCTCTTGCTGTGCCTCTCGAACTGCAGCTTTTCGCTTTCTACCTGACTCTCCTCCTGAGGTTCCTCCTAAAATTGTTCGAATCTCGAGCGGTGGGTTGGCACCACCCTTACCTTGGTCTGCTGGTAGTGGCCTCTTGCTTCTGTCATTCCCCATGAATTCATTGAGGTAA

Protein sequence

MCLSMDVASLVAHETTAVKLMESLTNRYEKPSANNKVYVVKKFFNMQMSEEASVNSYINEVTTLINQLKSVKIEFSDEVYAIQLLTSLLDSWETMKTPVSNSTGNNTLKFSEVCDLAIAEEIRRQGSNKESTVGSALVMTKGKDKVDEDNEPSSSRKKWKGRNEVECYYCHKKGHFKYQCRKFKEDQKRKPEANIVEEVVLACVESDTKYSNHSSGWILDSAASVHIASDRSLFTSFTGGHHGLVRMGNGRTSKTRGIGDVSLKTECGGKLVLRDVRYVPNIKMNLISIGKLADDGYMSSFSVNSNFGEFSSSRSEYIPCPPGSCCASRTAAFRFLPDSPPEVPPKIVRISSGGLAPPLPWSAGSGLLLLSFPMNSLR

Homology

[Close]

BLAST of Lag0001159 vs. NCBI nr
Match: TKS09800.1 (hypothetical protein D5086_0000089010 [Populus alba])

HSP 1 Score: 217.2 bits (552), Expect = 2.4e-52
Identity = 130/310 (41.94%), Postives = 185/310 (59.68%), Query Frame = 0


Query: 1   MCLSMDVASLVAHETTAVKLMESLTNRYEKPSANNKVYVVKKFFNMQMSEEASVNSYINE 60
           + LS  VA  V  E +  KLME+L+  YEKPSANNKV+++KK FN++M E  SV  ++N 
Sbjct: 64  LSLSRRVAHNVTKEKSTAKLMEALSGMYEKPSANNKVHLMKKLFNLKMVENTSVTQHLNN 123

Query: 61  VTTLINQLKSVKIEFSDEVYAIQLLTSLLDSWETMKTPVSNSTGNNTLKFSEVCDLAIAE 120
             T+ NQL SV+IEF DE+ A+ LL SL  SWE M+T VSNS G + LK+ ++ DL +AE
Sbjct: 124 FNTITNQLSSVEIEFDDEIRALILLASLPSSWEGMRTAVSNSAGKSKLKYDDIRDLILAE 183

Query: 121 EIRRQGSNKESTVGSAL-VMTKGK--DKVDEDNEPSS---SRKKWKGRNEVECYYCHKKG 180
           E+RR+ S + S+ GSAL + T+G+  D+        S   S+ K+  R +VEC+ C K G
Sbjct: 184 EVRRKDSGESSSSGSALNINTRGRRHDRGSSRGRSKSRYRSKSKFGSRKQVECWNCGKPG 243

Query: 181 HFKYQCRKFKEDQKRKPEANI--VEEVVLACVESDTKYSNHSSGWILDSAASVHIASDRS 240
           HF   C K K+ +     A    V++ ++  V+S          WILDS AS H      
Sbjct: 244 HFSRNCTKPKKPEADSANATTDEVQDALILAVQSQI------DEWILDSGASFHCTPHNE 303

Query: 241 LFTSFTGGHHGLVRMGNGRTSKTRGIGDVSLKTECGGKLVLRDVRYVPNIKMNLISIGKL 300
           +  ++ GG HG+V + +G   K  GIGDV +KT  G    L++VR+VP +K  LIS+G+L
Sbjct: 304 MLQNYVGGDHGVVYLADGTPLKIVGIGDVQIKTMNGSIWTLQNVRHVPELKKKLISVGQL 363

Query: 301 ADDGYMSSFS 303
            D G+   F+
Sbjct: 364 DDSGHSILFA 367

[Close]

BLAST of Lag0001159 vs. NCBI nr
Match: TKS02608.1 (hypothetical protein D5086_0000161380 [Populus alba])

HSP 1 Score: 216.9 bits (551), Expect = 3.1e-52
Identity = 130/310 (41.94%), Postives = 185/310 (59.68%), Query Frame = 0


Query: 1   MCLSMDVASLVAHETTAVKLMESLTNRYEKPSANNKVYVVKKFFNMQMSEEASVNSYINE 60
           + LS  VA  V  E +  KLME+L+  YEKPSANNKV+++KK FN++M E  SV  ++N 
Sbjct: 64  LSLSRRVAHNVTKEKSTAKLMEALSGMYEKPSANNKVHLMKKLFNLKMVENTSVTQHLNN 123

Query: 61  VTTLINQLKSVKIEFSDEVYAIQLLTSLLDSWETMKTPVSNSTGNNTLKFSEVCDLAIAE 120
             T+ NQL SV+IEF DE+ A+ LL SL  SWE M+T VSNS G + LK+ ++ DL +AE
Sbjct: 124 FNTITNQLSSVEIEFDDEIRALILLASLPSSWEGMRTAVSNSAGKSKLKYDDIRDLILAE 183

Query: 121 EIRRQGSNKESTVGSAL-VMTKGK--DKVDEDNEPSS---SRKKWKGRNEVECYYCHKKG 180
           E+RR+ S + S+ GSAL + T+G+  D+        S   S+ K+  R +VEC+ C K G
Sbjct: 184 EVRRKDSGESSSSGSALNINTRGRRHDRGSSRGRSKSRYRSKSKFGSRKQVECWNCGKPG 243

Query: 181 HFKYQCRKFKEDQKRKPEANI--VEEVVLACVESDTKYSNHSSGWILDSAASVHIASDRS 240
           HF   C K K+ +     A    V++ ++  V+S          WILDS AS H      
Sbjct: 244 HFSRNCTKPKKPEADSANATTDEVQDALILVVQSQI------DEWILDSGASFHCTPHNE 303

Query: 241 LFTSFTGGHHGLVRMGNGRTSKTRGIGDVSLKTECGGKLVLRDVRYVPNIKMNLISIGKL 300
           +  ++ GG HG+V + +G   K  GIGDV +KT  G    L++VR+VP +K  LIS+G+L
Sbjct: 304 MLQNYVGGDHGVVYLADGTPLKIVGIGDVQIKTMNGSIWTLQNVRHVPELKKKLISVGQL 363

Query: 301 ADDGYMSSFS 303
            D G+   F+
Sbjct: 364 DDSGHSILFA 367

[Close]

BLAST of Lag0001159 vs. NCBI nr
Match: TKS13843.1 (hypothetical protein D5086_0000049350 [Populus alba])

HSP 1 Score: 216.1 bits (549), Expect = 5.3e-52
Identity = 129/310 (41.61%), Postives = 184/310 (59.35%), Query Frame = 0


Query: 1   MCLSMDVASLVAHETTAVKLMESLTNRYEKPSANNKVYVVKKFFNMQMSEEASVNSYINE 60
           + LS  VA  V  E +  KLME+L+  YEKPSANNKV+++KK FN++M E  SV  ++N 
Sbjct: 64  LSLSRRVAHNVTKEKSTAKLMEALSGMYEKPSANNKVHLMKKLFNLKMVENTSVTQHLNN 123

Query: 61  VTTLINQLKSVKIEFSDEVYAIQLLTSLLDSWETMKTPVSNSTGNNTLKFSEVCDLAIAE 120
             T+ NQL SV+IEF DE+ A+ LL SL  SWE M+T VSNS G + LK+ ++ DL +AE
Sbjct: 124 FNTITNQLSSVEIEFDDEIRALILLASLPSSWEGMRTAVSNSAGKSKLKYDDIRDLILAE 183

Query: 121 EIRRQGSNKESTVGSAL-VMTKGK--DKVDEDNEPSS---SRKKWKGRNEVECYYCHKKG 180
           E+RR+ S + S+ GSAL + T+G+  D+        S   S+ K+  R +VEC+ C K G
Sbjct: 184 EVRRKDSGESSSSGSALNINTRGRRHDRGSSRGRSKSRYRSKSKFGSRKQVECWNCGKPG 243

Query: 181 HFKYQCRKFKEDQKRKPEANI--VEEVVLACVESDTKYSNHSSGWILDSAASVHIASDRS 240
           HF   C K K+ +     A    V++ ++  V+S          WILDS AS H      
Sbjct: 244 HFSRNCTKPKKPEADSANATTDEVQDALILAVQSQI------DEWILDSGASFHCTPHNE 303

Query: 241 LFTSFTGGHHGLVRMGNGRTSKTRGIGDVSLKTECGGKLVLRDVRYVPNIKMNLISIGKL 300
           +  ++ GG HG++ + +G      GIGDV +KT  G    L++VR+VP +K  LIS+G+L
Sbjct: 304 MLQNYVGGDHGVLYLADGTPLNIVGIGDVQIKTMNGSIWTLQNVRHVPELKKKLISVGQL 363

Query: 301 ADDGYMSSFS 303
            D GY   F+
Sbjct: 364 DDSGYSILFA 367

[Close]

BLAST of Lag0001159 vs. NCBI nr
Match: TKR89927.1 (hypothetical protein D5086_0000238200 [Populus alba])

HSP 1 Score: 214.9 bits (546), Expect = 1.2e-51
Identity = 128/310 (41.29%), Postives = 184/310 (59.35%), Query Frame = 0


Query: 1   MCLSMDVASLVAHETTAVKLMESLTNRYEKPSANNKVYVVKKFFNMQMSEEASVNSYINE 60
           + LS  VA  V  E +  KLME+L+  YEKPSANNKV+++KK FN++M E  SV  ++N 
Sbjct: 64  LSLSRRVAHNVTKEKSTAKLMEALSGMYEKPSANNKVHLMKKLFNLKMVENTSVTQHLNN 123

Query: 61  VTTLINQLKSVKIEFSDEVYAIQLLTSLLDSWETMKTPVSNSTGNNTLKFSEVCDLAIAE 120
             T+ NQL SV+IEF DE+ A+ LL SL  SWE M+T VSNS G + LK+ ++ DL +AE
Sbjct: 124 FNTITNQLSSVEIEFDDEIRALILLASLPSSWEGMRTAVSNSAGKSKLKYDDIRDLILAE 183

Query: 121 EIRRQGSNKESTVGSAL-VMTKGK--DKVDEDNEPSS---SRKKWKGRNEVECYYCHKKG 180
           E+RR+ S + S+ GSAL + T+G+  D+        S   S+ K+  R +VEC+ C K G
Sbjct: 184 EVRRKDSGESSSSGSALNINTRGRRHDRGSSRGRSKSRYRSKSKFGSRKQVECWNCGKPG 243

Query: 181 HFKYQCRKFKEDQKRKPEANI--VEEVVLACVESDTKYSNHSSGWILDSAASVHIASDRS 240
           HF   C K K+ +     A    V++ ++  V+S          WILDS AS H      
Sbjct: 244 HFSRNCTKPKKPEADSANATTDEVQDALILAVQSQI------DEWILDSGASFHCTPHNE 303

Query: 241 LFTSFTGGHHGLVRMGNGRTSKTRGIGDVSLKTECGGKLVLRDVRYVPNIKMNLISIGKL 300
           +  ++ GG HG+V + +G      GIGDV +KT  G    L+++R+VP +K  LIS+G+L
Sbjct: 304 MLQNYVGGDHGVVYLADGTPLNIVGIGDVQIKTMNGSIWTLQNIRHVPELKKKLISVGQL 363

Query: 301 ADDGYMSSFS 303
            D G+   F+
Sbjct: 364 DDSGHSILFA 367

[Close]

BLAST of Lag0001159 vs. NCBI nr
Match: TKR86343.1 (hypothetical protein D5086_0000239010 [Populus alba])

HSP 1 Score: 214.5 bits (545), Expect = 1.5e-51
Identity = 129/310 (41.61%), Postives = 183/310 (59.03%), Query Frame = 0


Query: 1   MCLSMDVASLVAHETTAVKLMESLTNRYEKPSANNKVYVVKKFFNMQMSEEASVNSYINE 60
           + LS  VA  V  E +  KLME+L+  YEKPSANNKV+++KK FN++M E  SV  ++N 
Sbjct: 19  LSLSRRVAHNVTKEKSTAKLMEALSGMYEKPSANNKVHLMKKLFNLKMVENTSVTQHLNN 78

Query: 61  VTTLINQLKSVKIEFSDEVYAIQLLTSLLDSWETMKTPVSNSTGNNTLKFSEVCDLAIAE 120
             T+ NQL SV+IEF DE+ A+ LL SL  SWE M+T VSNS G + LK+ ++ DL +AE
Sbjct: 79  FNTITNQLSSVEIEFDDEIRALILLASLPSSWEGMRTAVSNSAGKSKLKYDDIRDLILAE 138

Query: 121 EIRRQGSNKESTVGSAL-VMTKGK--DKVDEDNEPSS---SRKKWKGRNEVECYYCHKKG 180
           E+RR+ S + S+ GSAL + T+G+  D+        S   S+ K+  R +VEC+ C K G
Sbjct: 139 EVRRKDSGESSSSGSALNINTRGRRHDRGSSRGRSKSRYRSKSKFGSRKQVECWNCGKPG 198

Query: 181 HFKYQCRKFKEDQKRKPEANI--VEEVVLACVESDTKYSNHSSGWILDSAASVHIASDRS 240
           HF   C K K+ +     A    V++ ++  V S          WILDS AS H      
Sbjct: 199 HFSRNCTKPKKPEADSANATTDEVQDALILAVHSQI------DEWILDSGASFHCTPHNE 258

Query: 241 LFTSFTGGHHGLVRMGNGRTSKTRGIGDVSLKTECGGKLVLRDVRYVPNIKMNLISIGKL 300
           +  ++ GG HG+V + +G      GIGDV +KT  G    L++VR+VP +K  LIS+G+L
Sbjct: 259 MLQNYVGGDHGVVYLADGTPLNIVGIGDVQIKTMNGSIWTLQNVRHVPELKKKLISVGQL 318

Query: 301 ADDGYMSSFS 303
            D G+   F+
Sbjct: 319 DDSGHSILFA 322

[Close]

BLAST of Lag0001159 vs. ExPASy Swiss-Prot
Match: P10978 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 138.3 bits (347), Expect = 1.8e-31
Identity = 109/319 (34.17%), Postives = 166/319 (52.04%), Query Frame = 0


Query: 3   LSMDVASLVAHETTAVKLMESLTNRYEKPSANNKVYVVKKFFNMQMSEEASVNSYINEVT 62
           LS DV + +  E TA  +   L + Y   +  NK+Y+ K+ + + MSE  +  S++N   
Sbjct: 67  LSDDVVNNIIDEDTARGIWTRLESLYMSKTLTNKLYLKKQLYALHMSEGTNFLSHLNVFN 126

Query: 63  TLINQLKSVKIEFSDEVYAIQLLTSLLDSWETMKTPVSNSTGNNTLKFSEVCD-LAIAEE 122
            LI QL ++ ++  +E  AI LL SL  S++ + T + +  G  T++  +V   L + E+
Sbjct: 127 GLITQLANLGVKIEEEDKAILLLNSLPSSYDNLATTILH--GKTTIELKDVTSALLLNEK 186

Query: 123 IRRQGSNKESTVGSALVMT-KGKDKVDEDNEPSSSRKKWKGRNEVE-----CYYCHKKGH 182
           +R++  N+    G AL+   +G+      N    S  + K +N  +     CY C++ GH
Sbjct: 187 MRKKPENQ----GQALITEGRGRSYQRSSNNYGRSGARGKSKNRSKSRVRNCYNCNQPGH 246

Query: 183 FKYQC---RKFK-EDQKRKPEANIV------EEVVLACVESD--TKYSNHSSGWILDSAA 242
           FK  C   RK K E   +K + N        + VVL   E +     S   S W++D+AA
Sbjct: 247 FKRDCPNPRKGKGETSGQKNDDNTAAMVQNNDNVVLFINEEEECMHLSGPESEWVVDTAA 306

Query: 243 SVHIASDRSLFTSFTGGHHGLVRMGNGRTSKTRGIGDVSLKTECGGKLVLRDVRYVPNIK 302
           S H    R LF  +  G  G V+MGN   SK  GIGD+ +KT  G  LVL+DVR+VP+++
Sbjct: 307 SHHATPVRDLFCRYVAGDFGTVKMGNTSYSKIAGIGDICIKTNVGCTLVLKDVRHVPDLR 366

[Close]

BLAST of Lag0001159 vs. ExPASy Swiss-Prot
Match: P25601 (Putative transposon Ty5-1 protein YCL075W OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY5B PE=5 SV=1)

HSP 1 Score: 54.3 bits (129), Expect = 3.5e-06
Identity = 32/87 (36.78%), Postives = 47/87 (54.02%), Query Frame = 0


Query: 203 CVESDTKYSNHSSGWILDSAASVHIASDRSLFTSFTGGHHGLVRMGNGRTSKTRGIGDVS 262
           C+ S T  +  SS WI D+  + H+  DRS+F+SFT         G G +    G G V+
Sbjct: 65  CMLSSTVPATKSSEWIFDTGCTSHMCHDRSIFSSFTRSSRKDFVRGVGGSIPIMGSGTVN 124

Query: 263 LKTECGGKLVLRDVRYVPNIKMNLISI 290
           +     G + L DV YVP++ +NLIS+
Sbjct: 125 I-----GTVQLHDVSYVPDLPVNLISV 146

[Close]

BLAST of Lag0001159 vs. ExPASy Swiss-Prot
Match: Q94HW2 (Retrovirus-related Pol polyprotein from transposon RE1 OS=Arabidopsis thaliana OX=3702 GN=RE1 PE=2 SV=1)

HSP 1 Score: 49.7 bits (117), Expect = 8.6e-05
Identity = 74/324 (22.84%), Postives = 132/324 (40.74%), Query Frame = 0


Query: 3   LSMDVASLVAHETTAVKLMESLTNRYEKPSANNKVYVVKKFFNMQMSEEASVNSYINEVT 62
           +SM V   V+  TTA ++ E+L   Y  PS  + V  ++           +++ Y+  + 
Sbjct: 92  ISMSVQPAVSRATTAAQIWETLRKIYANPSYGH-VTQLRTQLKQWTKGTKTIDDYMQGLV 151

Query: 63  TLINQLKSVKIEFSDEVYAIQLLTSLLDSWETM------------KTPVSNSTGNNTLKF 122
           T  +QL  +      +    ++L +L + ++ +             T +     N+  K 
Sbjct: 152 TRFDQLALLGKPMDHDEQVERVLENLPEEYKPVIDQIAAKDTPPTLTEIHERLLNHESKI 211

Query: 123 SEVCDLAIAEEIRRQGSNKESTVGSALVMTKGKDKVDEDNEPSSSRKKWK--------GR 182
             V    +        S++ +T  +        ++ D  N  ++S K W+          
Sbjct: 212 LAVSSATVIPITANAVSHRNTTTTNNNNNGNRNNRYDNRNNNNNS-KPWQQSSTNFHPNN 271

Query: 183 NEV-----ECYYCHKKGHFKYQCRKFK----EDQKRKPEANIVEEVVLACVESDTKYSNH 242
           N+      +C  C  +GH   +C + +        ++P +        A +   + YS  
Sbjct: 272 NQSKPYLGKCQICGVQGHSAKRCSQLQHFLSSVNSQQPPSPFTPWQPRANLALGSPYS-- 331

Query: 243 SSGWILDSAASVHIASD---RSLFTSFTGGHHGLVRMGNGRTSKTRGIGDVSLKTECGGK 295
           S+ W+LDS A+ HI SD    SL   +TGG    V + +G T      G  SL T+    
Sbjct: 332 SNNWLLDSGATHHITSDFNNLSLHQPYTGGDD--VMVADGSTIPISHTGSTSLSTK-SRP 391

[Close]

BLAST of Lag0001159 vs. ExPASy TrEMBL
Match: A0A4U5QGR0 (Uncharacterized protein OS=Populus alba OX=43335 GN=D5086_0000089010 PE=4 SV=1)

HSP 1 Score: 217.2 bits (552), Expect = 1.1e-52
Identity = 130/310 (41.94%), Postives = 185/310 (59.68%), Query Frame = 0


Query: 1   MCLSMDVASLVAHETTAVKLMESLTNRYEKPSANNKVYVVKKFFNMQMSEEASVNSYINE 60
           + LS  VA  V  E +  KLME+L+  YEKPSANNKV+++KK FN++M E  SV  ++N 
Sbjct: 64  LSLSRRVAHNVTKEKSTAKLMEALSGMYEKPSANNKVHLMKKLFNLKMVENTSVTQHLNN 123

Query: 61  VTTLINQLKSVKIEFSDEVYAIQLLTSLLDSWETMKTPVSNSTGNNTLKFSEVCDLAIAE 120
             T+ NQL SV+IEF DE+ A+ LL SL  SWE M+T VSNS G + LK+ ++ DL +AE
Sbjct: 124 FNTITNQLSSVEIEFDDEIRALILLASLPSSWEGMRTAVSNSAGKSKLKYDDIRDLILAE 183

Query: 121 EIRRQGSNKESTVGSAL-VMTKGK--DKVDEDNEPSS---SRKKWKGRNEVECYYCHKKG 180
           E+RR+ S + S+ GSAL + T+G+  D+        S   S+ K+  R +VEC+ C K G
Sbjct: 184 EVRRKDSGESSSSGSALNINTRGRRHDRGSSRGRSKSRYRSKSKFGSRKQVECWNCGKPG 243

Query: 181 HFKYQCRKFKEDQKRKPEANI--VEEVVLACVESDTKYSNHSSGWILDSAASVHIASDRS 240
           HF   C K K+ +     A    V++ ++  V+S          WILDS AS H      
Sbjct: 244 HFSRNCTKPKKPEADSANATTDEVQDALILAVQSQI------DEWILDSGASFHCTPHNE 303

Query: 241 LFTSFTGGHHGLVRMGNGRTSKTRGIGDVSLKTECGGKLVLRDVRYVPNIKMNLISIGKL 300
           +  ++ GG HG+V + +G   K  GIGDV +KT  G    L++VR+VP +K  LIS+G+L
Sbjct: 304 MLQNYVGGDHGVVYLADGTPLKIVGIGDVQIKTMNGSIWTLQNVRHVPELKKKLISVGQL 363

Query: 301 ADDGYMSSFS 303
            D G+   F+
Sbjct: 364 DDSGHSILFA 367

[Close]

BLAST of Lag0001159 vs. ExPASy TrEMBL
Match: A0A4U5PY83 (CCHC-type domain-containing protein OS=Populus alba OX=43335 GN=D5086_0000161380 PE=4 SV=1)

HSP 1 Score: 216.9 bits (551), Expect = 1.5e-52
Identity = 130/310 (41.94%), Postives = 185/310 (59.68%), Query Frame = 0


Query: 1   MCLSMDVASLVAHETTAVKLMESLTNRYEKPSANNKVYVVKKFFNMQMSEEASVNSYINE 60
           + LS  VA  V  E +  KLME+L+  YEKPSANNKV+++KK FN++M E  SV  ++N 
Sbjct: 64  LSLSRRVAHNVTKEKSTAKLMEALSGMYEKPSANNKVHLMKKLFNLKMVENTSVTQHLNN 123

Query: 61  VTTLINQLKSVKIEFSDEVYAIQLLTSLLDSWETMKTPVSNSTGNNTLKFSEVCDLAIAE 120
             T+ NQL SV+IEF DE+ A+ LL SL  SWE M+T VSNS G + LK+ ++ DL +AE
Sbjct: 124 FNTITNQLSSVEIEFDDEIRALILLASLPSSWEGMRTAVSNSAGKSKLKYDDIRDLILAE 183

Query: 121 EIRRQGSNKESTVGSAL-VMTKGK--DKVDEDNEPSS---SRKKWKGRNEVECYYCHKKG 180
           E+RR+ S + S+ GSAL + T+G+  D+        S   S+ K+  R +VEC+ C K G
Sbjct: 184 EVRRKDSGESSSSGSALNINTRGRRHDRGSSRGRSKSRYRSKSKFGSRKQVECWNCGKPG 243

Query: 181 HFKYQCRKFKEDQKRKPEANI--VEEVVLACVESDTKYSNHSSGWILDSAASVHIASDRS 240
           HF   C K K+ +     A    V++ ++  V+S          WILDS AS H      
Sbjct: 244 HFSRNCTKPKKPEADSANATTDEVQDALILVVQSQI------DEWILDSGASFHCTPHNE 303

Query: 241 LFTSFTGGHHGLVRMGNGRTSKTRGIGDVSLKTECGGKLVLRDVRYVPNIKMNLISIGKL 300
           +  ++ GG HG+V + +G   K  GIGDV +KT  G    L++VR+VP +K  LIS+G+L
Sbjct: 304 MLQNYVGGDHGVVYLADGTPLKIVGIGDVQIKTMNGSIWTLQNVRHVPELKKKLISVGQL 363

Query: 301 ADDGYMSSFS 303
            D G+   F+
Sbjct: 364 DDSGHSILFA 367

[Close]

BLAST of Lag0001159 vs. ExPASy TrEMBL
Match: A0A4U5QS59 (CCHC-type domain-containing protein OS=Populus alba OX=43335 GN=D5086_0000049350 PE=4 SV=1)

HSP 1 Score: 216.1 bits (549), Expect = 2.6e-52
Identity = 129/310 (41.61%), Postives = 184/310 (59.35%), Query Frame = 0


Query: 1   MCLSMDVASLVAHETTAVKLMESLTNRYEKPSANNKVYVVKKFFNMQMSEEASVNSYINE 60
           + LS  VA  V  E +  KLME+L+  YEKPSANNKV+++KK FN++M E  SV  ++N 
Sbjct: 64  LSLSRRVAHNVTKEKSTAKLMEALSGMYEKPSANNKVHLMKKLFNLKMVENTSVTQHLNN 123

Query: 61  VTTLINQLKSVKIEFSDEVYAIQLLTSLLDSWETMKTPVSNSTGNNTLKFSEVCDLAIAE 120
             T+ NQL SV+IEF DE+ A+ LL SL  SWE M+T VSNS G + LK+ ++ DL +AE
Sbjct: 124 FNTITNQLSSVEIEFDDEIRALILLASLPSSWEGMRTAVSNSAGKSKLKYDDIRDLILAE 183

Query: 121 EIRRQGSNKESTVGSAL-VMTKGK--DKVDEDNEPSS---SRKKWKGRNEVECYYCHKKG 180
           E+RR+ S + S+ GSAL + T+G+  D+        S   S+ K+  R +VEC+ C K G
Sbjct: 184 EVRRKDSGESSSSGSALNINTRGRRHDRGSSRGRSKSRYRSKSKFGSRKQVECWNCGKPG 243

Query: 181 HFKYQCRKFKEDQKRKPEANI--VEEVVLACVESDTKYSNHSSGWILDSAASVHIASDRS 240
           HF   C K K+ +     A    V++ ++  V+S          WILDS AS H      
Sbjct: 244 HFSRNCTKPKKPEADSANATTDEVQDALILAVQSQI------DEWILDSGASFHCTPHNE 303

Query: 241 LFTSFTGGHHGLVRMGNGRTSKTRGIGDVSLKTECGGKLVLRDVRYVPNIKMNLISIGKL 300
           +  ++ GG HG++ + +G      GIGDV +KT  G    L++VR+VP +K  LIS+G+L
Sbjct: 304 MLQNYVGGDHGVLYLADGTPLNIVGIGDVQIKTMNGSIWTLQNVRHVPELKKKLISVGQL 363

Query: 301 ADDGYMSSFS 303
            D GY   F+
Sbjct: 364 DDSGYSILFA 367

[Close]

BLAST of Lag0001159 vs. ExPASy TrEMBL
Match: A0A4U5P1P0 (CCHC-type domain-containing protein OS=Populus alba OX=43335 GN=D5086_0000238200 PE=4 SV=1)

HSP 1 Score: 214.9 bits (546), Expect = 5.7e-52
Identity = 128/310 (41.29%), Postives = 184/310 (59.35%), Query Frame = 0


Query: 1   MCLSMDVASLVAHETTAVKLMESLTNRYEKPSANNKVYVVKKFFNMQMSEEASVNSYINE 60
           + LS  VA  V  E +  KLME+L+  YEKPSANNKV+++KK FN++M E  SV  ++N 
Sbjct: 64  LSLSRRVAHNVTKEKSTAKLMEALSGMYEKPSANNKVHLMKKLFNLKMVENTSVTQHLNN 123

Query: 61  VTTLINQLKSVKIEFSDEVYAIQLLTSLLDSWETMKTPVSNSTGNNTLKFSEVCDLAIAE 120
             T+ NQL SV+IEF DE+ A+ LL SL  SWE M+T VSNS G + LK+ ++ DL +AE
Sbjct: 124 FNTITNQLSSVEIEFDDEIRALILLASLPSSWEGMRTAVSNSAGKSKLKYDDIRDLILAE 183

Query: 121 EIRRQGSNKESTVGSAL-VMTKGK--DKVDEDNEPSS---SRKKWKGRNEVECYYCHKKG 180
           E+RR+ S + S+ GSAL + T+G+  D+        S   S+ K+  R +VEC+ C K G
Sbjct: 184 EVRRKDSGESSSSGSALNINTRGRRHDRGSSRGRSKSRYRSKSKFGSRKQVECWNCGKPG 243

Query: 181 HFKYQCRKFKEDQKRKPEANI--VEEVVLACVESDTKYSNHSSGWILDSAASVHIASDRS 240
           HF   C K K+ +     A    V++ ++  V+S          WILDS AS H      
Sbjct: 244 HFSRNCTKPKKPEADSANATTDEVQDALILAVQSQI------DEWILDSGASFHCTPHNE 303

Query: 241 LFTSFTGGHHGLVRMGNGRTSKTRGIGDVSLKTECGGKLVLRDVRYVPNIKMNLISIGKL 300
           +  ++ GG HG+V + +G      GIGDV +KT  G    L+++R+VP +K  LIS+G+L
Sbjct: 304 MLQNYVGGDHGVVYLADGTPLNIVGIGDVQIKTMNGSIWTLQNIRHVPELKKKLISVGQL 363

Query: 301 ADDGYMSSFS 303
            D G+   F+
Sbjct: 364 DDSGHSILFA 367

[Close]

BLAST of Lag0001159 vs. ExPASy TrEMBL
Match: A0A4U5NTA0 (CCHC-type domain-containing protein OS=Populus alba OX=43335 GN=D5086_0000239010 PE=3 SV=1)

HSP 1 Score: 214.5 bits (545), Expect = 7.4e-52
Identity = 129/310 (41.61%), Postives = 183/310 (59.03%), Query Frame = 0


Query: 1   MCLSMDVASLVAHETTAVKLMESLTNRYEKPSANNKVYVVKKFFNMQMSEEASVNSYINE 60
           + LS  VA  V  E +  KLME+L+  YEKPSANNKV+++KK FN++M E  SV  ++N 
Sbjct: 19  LSLSRRVAHNVTKEKSTAKLMEALSGMYEKPSANNKVHLMKKLFNLKMVENTSVTQHLNN 78

Query: 61  VTTLINQLKSVKIEFSDEVYAIQLLTSLLDSWETMKTPVSNSTGNNTLKFSEVCDLAIAE 120
             T+ NQL SV+IEF DE+ A+ LL SL  SWE M+T VSNS G + LK+ ++ DL +AE
Sbjct: 79  FNTITNQLSSVEIEFDDEIRALILLASLPSSWEGMRTAVSNSAGKSKLKYDDIRDLILAE 138

Query: 121 EIRRQGSNKESTVGSAL-VMTKGK--DKVDEDNEPSS---SRKKWKGRNEVECYYCHKKG 180
           E+RR+ S + S+ GSAL + T+G+  D+        S   S+ K+  R +VEC+ C K G
Sbjct: 139 EVRRKDSGESSSSGSALNINTRGRRHDRGSSRGRSKSRYRSKSKFGSRKQVECWNCGKPG 198

Query: 181 HFKYQCRKFKEDQKRKPEANI--VEEVVLACVESDTKYSNHSSGWILDSAASVHIASDRS 240
           HF   C K K+ +     A    V++ ++  V S          WILDS AS H      
Sbjct: 199 HFSRNCTKPKKPEADSANATTDEVQDALILAVHSQI------DEWILDSGASFHCTPHNE 258

Query: 241 LFTSFTGGHHGLVRMGNGRTSKTRGIGDVSLKTECGGKLVLRDVRYVPNIKMNLISIGKL 300
           +  ++ GG HG+V + +G      GIGDV +KT  G    L++VR+VP +K  LIS+G+L
Sbjct: 259 MLQNYVGGDHGVVYLADGTPLNIVGIGDVQIKTMNGSIWTLQNVRHVPELKKKLISVGQL 318

Query: 301 ADDGYMSSFS 303
            D G+   F+
Sbjct: 319 DDSGHSILFA 322

[Close]

BLAST of Lag0001159 vs. TAIR 10
Match: AT3G21000.1 (Gag-Pol-related retrotransposon family protein )

HSP 1 Score: 55.1 bits (131), Expect = 1.4e-07
Identity = 55/260 (21.15%), Postives = 107/260 (41.15%), Query Frame = 0


Query: 41  KKFFNMQMSEEASVNSYINEVTTLINQLKSVKIEFSDEVYAIQLLTSLLDSWETMKTPVS 100
           K+  +++M ++ S +SY+++   ++ +L   K+E SD      + T+L  S++ + + + 
Sbjct: 124 KQLEDLKMVDKESGSSYLDKALEILERLGRAKLEKSDYEICKNVFTTLSGSFDGLDSMLE 183

Query: 101 NSTGNNTLKFSEVCDLAIAEEIRRQGSNKESTVGSALVMTKGKDKVDEDNEPSSSRKKWK 160
                + +    + +       R   S+ E  +   L          +D    S  +KW 
Sbjct: 184 ELIDVHKMTSKSLVEYFY---YRVHESSTEEAIFGLL----------KDLRLKSKSEKW- 243

Query: 161 GRNEVECYYCHKKGHFKYQCRKFKEDQKRKPEANIVEEVVLACVESDTKYSNHSSGWILD 220
                 C  C+K  H +  C+      K + E  IV +  L  V +    +     WI+ 
Sbjct: 244 ------CGLCYKNNHNQEDCKFRIHTDKEEKEDEIVVDYRLETVPNLGAKTYDDDIWIIH 303

Query: 221 SAASVHIASDRSLFTSFTGGHHGLVRMGNGRTSKTRGIGDVSLKTECGGKLVLRDVRYVP 280
             A +++      FT+        V   +G      G GDV ++ + G K  +R+V +VP
Sbjct: 304 KMAPINMTPYVKYFTTLDRTFKATVGTVDGTVLLVEGKGDVKIRMKEGKKKTIRNVIFVP 363

Query: 281 NIKMNLISIGKLADDGYMSS 301
            +  N++S GK+    Y  S
Sbjct: 364 GLNRNVLSFGKMVSKRYSIS 363

[Close]

BLAST of Lag0001159 vs. TAIR 10
Match: AT3G20980.1 (Gag-Pol-related retrotransposon family protein )

HSP 1 Score: 50.8 bits (120), Expect = 2.7e-06
Identity = 33/109 (30.28%), Postives = 56/109 (51.38%), Query Frame = 0


Query: 194 NIVEEVVLACVESDTKYSNHSSGWILDSAASVHIASDRSLFTSFTGGHHGLVRMGNGRTS 253
           +++ EV     E  +KY+ H + W++ S  S H+      FT+        V+  +G  S
Sbjct: 246 DLLNEVPDHVAECFSKYTFHENIWLISSTNSNHMTPHVKFFTTLDRSRKCKVKFISGDKS 305

Query: 254 KT-----RGIGDVSLKTECGGKLVLRDVRYVPNIKMNLISIGKLADDGY 298
           +T      GIGDV+  T  G K  +++V YVP I+ N +S+ +L  +G+
Sbjct: 306 ETTVAMVEGIGDVTFITNEGNK-TIKNVLYVPGIEGNALSVSQLKRNGF 353

The following BLAST results are available for this feature:

BLAST of Lag0001159 vs. NCBI nr


Match Name	E-value	Identity	Description
TKS09800.1	2.4e-52	41.94	hypothetical protein D5086_0000089010 [Populus alba]	[more]
TKS02608.1	3.1e-52	41.94	hypothetical protein D5086_0000161380 [Populus alba]	[more]
TKS13843.1	5.3e-52	41.61	hypothetical protein D5086_0000049350 [Populus alba]	[more]
TKR89927.1	1.2e-51	41.29	hypothetical protein D5086_0000238200 [Populus alba]	[more]
TKR86343.1	1.5e-51	41.61	hypothetical protein D5086_0000239010 [Populus alba]	[more]

BLAST of Lag0001159 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P10978	1.8e-31	34.17	Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Nicotiana tabacum...	[more]
P25601	3.5e-06	36.78	Putative transposon Ty5-1 protein YCL075W OS=Saccharomyces cerevisiae (strain AT...	[more]
Q94HW2	8.6e-05	22.84	Retrovirus-related Pol polyprotein from transposon RE1 OS=Arabidopsis thaliana O...	[more]

BLAST of Lag0001159 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A4U5QGR0	1.1e-52	41.94	Uncharacterized protein OS=Populus alba OX=43335 GN=D5086_0000089010 PE=4 SV=1	[more]
A0A4U5PY83	1.5e-52	41.94	CCHC-type domain-containing protein OS=Populus alba OX=43335 GN=D5086_0000161380...	[more]
A0A4U5QS59	2.6e-52	41.61	CCHC-type domain-containing protein OS=Populus alba OX=43335 GN=D5086_0000049350...	[more]
A0A4U5P1P0	5.7e-52	41.29	CCHC-type domain-containing protein OS=Populus alba OX=43335 GN=D5086_0000238200...	[more]
A0A4U5NTA0	7.4e-52	41.61	CCHC-type domain-containing protein OS=Populus alba OX=43335 GN=D5086_0000239010...	[more]

BLAST of Lag0001159 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G21000.1	1.4e-07	21.15	Gag-Pol-related retrotransposon family protein	[more]
AT3G20980.1	2.7e-06	30.28	Gag-Pol-related retrotransposon family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PFAM	PF14223	Retrotran_gag_2	coord: 3..125 e-value: 6.8E-22 score: 77.7
None	No IPR available	GENE3D	4.10.60.10		coord: 139..196 e-value: 6.3E-7 score: 31.2
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 141..157
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 129..157
None	No IPR available	PANTHER	PTHR34676:SF1	ZINC FINGER, CCHC-TYPE, TUBBY C-TERMINAL-LIKE DOMAIN PROTEIN-RELATED	coord: 3..214
None	No IPR available	PANTHER	PTHR34676	FAMILY NOT NAMED	coord: 3..214
IPR001711	Phospholipase C, phosphatidylinositol-specific, Y domain	PROSITE	PS50008	PIPLC_Y_DOMAIN	coord: 343..372 score: 7.6385
IPR001878	Zinc finger, CCHC-type	PROSITE	PS50158	ZF_CCHC	coord: 167..182 score: 8.77913
IPR036875	Zinc finger, CCHC-type superfamily	SUPERFAMILY	57756	Retrovirus zinc finger-like domains	coord: 150..188

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0001159.1	Lag0001159.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0035556	intracellular signal transduction
biological_process	GO:0006629	lipid metabolic process
biological_process	GO:0007165	signal transduction
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0004435	phosphatidylinositol phospholipase C activity
molecular_function	GO:0016740	transferase activity
molecular_function	GO:0008270	zinc ion binding