Lag0001068 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0001068
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Receptor-like serine/threonine-protein kinase
Location	chr4: 23487560 .. 23490210 (-)
RNA-Seq Expression	Lag0001068
Synteny	Lag0001068

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAAGAAGTAGCAAGAACCCATCAATGGCGTCATCAAGTGCTGGTTTTGGCCTTCCCCTTCGTCAGCCATCCGACCTGCCTTCTCAACCTCATTCGACGACTCGCCGACGAGGTTCCAACCGTGAAATTCTCCTTCTTCAACACGTCGGCTTCCAACGCCGCCATCTTCAAGGACGCCGGACGAAATCACAACGTTTTCCCTTACAGCGTCGGCGACGGCCTGCCGGAGGGGTACGTGAGGCCACTTGGAATCCGGTTTTTCGACCCTTCGAGATCGTCGAGTTTTCGGAAGCTTTCTTGCCCGTCGAGATTTGGGGAGATGATTTTGTTTAAGAATCTAGTGTTCAAAAAATGGAAGGCAAAAGGGCAACAACGAAAAAAGAAAAATTAAAACGTATTTATTAAAAAATCCATAAATGCCTCAAAATCAGAAAATTTTTTTGTGGGATATCATTGAATGAAGAGAGATAGTATGGAGCAAACTTAATGAGGAGAGATGAAGCCAAGAAGGGTAAATTTGTCACAAAAAAAATGGAAAATCTAAATCAGAAAAAAAATTCCTATCTTGGACATTTTCGAAAAATGGGGTTGGAAGATGGTTATCTGACAAAATTAATGAGGAGAGATGAAGCCAAGAAGGGTAAATTTGTCACAAAAGAAATGGAAAATCTAAATCAGAAAAAATTTCCTATCTTGGACATTTTCGAAAAATGGGGTTGGAAGATGGTTATCTGACAAAATTTCTGTATTAAAAATGAGTTGGATTAAAGCCCAAATATAACTTAAGCCCAAAGGTTAGGCCCAAGCCCAAGCCCATGTGAGGTACATCAAACTCTATTTAGGCCACCATTCATTTTGGGCATTCAGACAGAGTCAGCGAAGCCAGAGCCCAGAGCATTCTCCCAAGGTCCAGAGTCTTCAGAAGTCAGAGAGTTCAGAGAATTTAGAAGATCCAAGATTCAGAATTCGATCATCTCAAGACTCAGAAGATCCAACGACTGAAAGACTCCAAAGAATCGACCGTTTCTTCATCAAGATACAAGTTGAAGATTCAACCTTCCTCCGTCTGAGATCAAGCTTGTCGGCCCTCATATTAGACTCTACATTTTGAGAGAAAGCATCAGAGGATAAATATTAGAGATTGTAACACTTCTACATACAAAATCAATAAAAATCAAGTTCATTTCCACGAATTAAATTTCCTTTGTCGAAATCTCGTGTGAACAAGTATGAACATGGAAGTTCTTGGGAGAATTTTGGCAGCATTTTAGGTTGAAGAATTCCACTAATTCTTATCAGCTTATCCTTGACAATAAATTCTGAGTGTTAGTGTAGAGGGAAGATAGGTTGAGGGACTTATAATTCTATCTCGCCTAACTAGAAGTTGCTCTAAGAAGACTATACATACACTCACGCAAGAATTTCTAGGAAAAAAATTGATTTGTATATTCATTGGACTATGGGTTTATGTCTCTTGATTGCAATAGGTATGACATTTTAGGCATTAAGGTTTGAATCATGGAATTGCAACCATTGAAGGGAGGGGGACATGGCCTCCAATCCCTGCTATTGATATGATACTTTTTAGGGAAAAATACACTTTTGGTCCCTGAGGTTTGGGTTAAGTGACACTTTGGTCCCTGAGTTTTCAAACTCAACAAAAATACCCCCGAGGTTTGAGATAAGGAGCAATTTGGTCCCTGCCGTCAAATTTTTTGTTAAACCCTAACGGTAAGCTGACGTGGCACTATTATTTTAATTAAAAAAATTTAATTTTTAATTTTTTTAAATAAAAAATATAAAAATATTAAATATATTATTATTTTATTATTATTTTTAAAATTCTCTCTCTATCTTCTCCATCTTCTTTCTTCTCCACCTTCTTTCTCCCTCCATCTTCTTCCTTCCCCCTCCCATCTTCTTCAGCTTCGGTGGCCGGCCGCTGCCGGCGAACGGCGGCGGTCACCGGAATTTTTTTTTTTTTCCTTCTTTCTTCTTCTTCTCTTCTTCTCTTCTTCCCTTCTTCTTCTTTCTCTTCCTTTCTTCTGTTTTTTCTTTTTAAAAGAAGGGAGTTTCGGGGAGGAGGAGCTCCTCCTCCCCGATTCGGGCGTCCTCTCTTTCGCCGCCCCTTTAGCCTCCCACTCCCTTTTCCCCAACTTCACAGCCTCCAATTTCCAATTCATCGACGTCGACGGTGCCTTTCTATCCTCTCTCAACAACACTTTCACTGCTTCAATCACTAATTCAAACTCCGACTCCTCTCTCTACTTCTTCCTCATCACCCATGTCGCTTCCAACACCTTCATTTGGTCCGCCAATCCAAACAACCTCGTCTCCACTTCCAGCCCTCTCACTCTCTCCGTCGCCAGCCTCTCCCACTACAACGACTCTGGCGCCCTCATCTGGTCCACTCCGCCGCTCCCCTCCCTTGTCGCCGCCATGCACCTCCTCGACTCCGACAACATCCTCCTCCTCGACCGCGCCAACGTTTCTCTCTGGGAGACCTTCGATCATCCCACGGACACGATCGTTGCCGGACAGCGTCTTAGAGTTCAAAATCCCCTGTTTCCGGCTGTCACTCAAGACGACATGTCGGTTGGGGCCGCTGAGTATCGGCTTCTTGTTACCGATGACGATTTGCTGCTGTAG

mRNA sequence

ATGAAAGAAGTAGCAAGAACCCATCAATGGCGTCATCAAGTGCTGGTTTTGGCCTTCCCCTTCGTCAGCCATCCGACCTGCCTTCTCAACCTCATTCGACGACTCGCCGACGAGGTTCCAACCGTGAAATTCTCCTTCTTCAACACGTCGGCTTCCAACGCCGCCATCTTCAAGGACGCCGGACGAAATCACAACGTTTTCCCTTACAGCGTCGGCGACGGCCTGCCGGAGGGGTACGTGAGGCCACTTGGAATCCGACAGAGTCAGCGAAGCCAGAGCCCAGAGCATTCTCCCAAGGTCCAGAGTCTTCAGAAGTCAGAGAGTTCAGAGAATTTAGAAGATCCAAGATTCAGAATTCGATCATCTCAAGACTCAGAAGATCCAACGACTGAAAGACTCCAAAGAATCGACCAAGGGAGTTTCGGGGAGGAGGAGCTCCTCCTCCCCGATTCGGGCGTCCTCTCTTTCGCCGCCCCTTTAGCCTCCCACTCCCTTTTCCCCAACTTCACAGCCTCCAATTTCCAATTCATCGACGTCGACGGTGCCTTTCTATCCTCTCTCAACAACACTTTCACTGCTTCAATCACTAATTCAAACTCCGACTCCTCTCTCTACTTCTTCCTCATCACCCATGTCGCTTCCAACACCTTCATTTGGTCCGCCAATCCAAACAACCTCGTCTCCACTTCCAGCCCTCTCACTCTCTCCGTCGCCAGCCTCTCCCACTACAACGACTCTGGCGCCCTCATCTGGTCCACTCCGCCGCTCCCCTCCCTTGTCGCCGCCATGCACCTCCTCGACTCCGACAACATCCTCCTCCTCGACCGCGCCAACGTTTCTCTCTGGGAGACCTTCGATCATCCCACGGACACGATCGTTGCCGGACAGCGTCTTAGAGTTCAAAATCCCCTGTTTCCGGCTGTCACTCAAGACGACATGTCGGTTGGGGCCGCTGAGTATCGGCTTCTTGTTACCGATGACGATTTGCTGCTGTAG

Coding sequence (CDS)

ATGAAAGAAGTAGCAAGAACCCATCAATGGCGTCATCAAGTGCTGGTTTTGGCCTTCCCCTTCGTCAGCCATCCGACCTGCCTTCTCAACCTCATTCGACGACTCGCCGACGAGGTTCCAACCGTGAAATTCTCCTTCTTCAACACGTCGGCTTCCAACGCCGCCATCTTCAAGGACGCCGGACGAAATCACAACGTTTTCCCTTACAGCGTCGGCGACGGCCTGCCGGAGGGGTACGTGAGGCCACTTGGAATCCGACAGAGTCAGCGAAGCCAGAGCCCAGAGCATTCTCCCAAGGTCCAGAGTCTTCAGAAGTCAGAGAGTTCAGAGAATTTAGAAGATCCAAGATTCAGAATTCGATCATCTCAAGACTCAGAAGATCCAACGACTGAAAGACTCCAAAGAATCGACCAAGGGAGTTTCGGGGAGGAGGAGCTCCTCCTCCCCGATTCGGGCGTCCTCTCTTTCGCCGCCCCTTTAGCCTCCCACTCCCTTTTCCCCAACTTCACAGCCTCCAATTTCCAATTCATCGACGTCGACGGTGCCTTTCTATCCTCTCTCAACAACACTTTCACTGCTTCAATCACTAATTCAAACTCCGACTCCTCTCTCTACTTCTTCCTCATCACCCATGTCGCTTCCAACACCTTCATTTGGTCCGCCAATCCAAACAACCTCGTCTCCACTTCCAGCCCTCTCACTCTCTCCGTCGCCAGCCTCTCCCACTACAACGACTCTGGCGCCCTCATCTGGTCCACTCCGCCGCTCCCCTCCCTTGTCGCCGCCATGCACCTCCTCGACTCCGACAACATCCTCCTCCTCGACCGCGCCAACGTTTCTCTCTGGGAGACCTTCGATCATCCCACGGACACGATCGTTGCCGGACAGCGTCTTAGAGTTCAAAATCCCCTGTTTCCGGCTGTCACTCAAGACGACATGTCGGTTGGGGCCGCTGAGTATCGGCTTCTTGTTACCGATGACGATTTGCTGCTGTAG

Protein sequence

MKEVARTHQWRHQVLVLAFPFVSHPTCLLNLIRRLADEVPTVKFSFFNTSASNAAIFKDAGRNHNVFPYSVGDGLPEGYVRPLGIRQSQRSQSPEHSPKVQSLQKSESSENLEDPRFRIRSSQDSEDPTTERLQRIDQGSFGEEELLLPDSGVLSFAAPLASHSLFPNFTASNFQFIDVDGAFLSSLNNTFTASITNSNSDSSLYFFLITHVASNTFIWSANPNNLVSTSSPLTLSVASLSHYNDSGALIWSTPPLPSLVAAMHLLDSDNILLLDRANVSLWETFDHPTDTIVAGQRLRVQNPLFPAVTQDDMSVGAAEYRLLVTDDDLLL

Homology

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BLAST of Lag0001068 vs. NCBI nr
Match: XP_022966534.1 (G-type lectin S-receptor-like serine/threonine-protein kinase At5g35370 [Cucurbita maxima])

HSP 1 Score: 245.0 bits (624), Expect = 9.3e-61
Identity = 134/187 (71.66%), Postives = 152/187 (81.28%), Query Frame = 0


Query: 146 LLLPDSGVLSFAAPLASHSLFPNFTASNFQFIDVDGAFLSSLNNTFTASITNSNSDSSLY 205
           LL P    LS +APLA H+++PNFTASNF+FID  GAFL+SLNNTFTASITNS SDSS Y
Sbjct: 28  LLFPFFTFLS-SAPLAFHAVYPNFTASNFKFIDDSGAFLASLNNTFTASITNSKSDSSKY 87

Query: 206 FFLITHVASNTFIWSANPNNLVSTSSPLTLSVASLS-HYNDSGALIWSTPPLPSLVAAMH 265
           FFLITHV S+T IWSANP N VS SSPLTLS A LS   +DSGAL+WSTPPLPS VAAMH
Sbjct: 88  FFLITHVVSDTIIWSANPYNPVSISSPLTLSPAGLSLSDDDSGALVWSTPPLPSPVAAMH 147

Query: 266 LLDSDNILLLDRANVSLWETFDHPTDTIVAGQRLRVQNPLFPAVTQDDMSVGAAEYRLLV 325
           LLDS N+LLLD ANV+LW++FD PTDTI+ GQRL V+NPLFPA T+DD       +RLL+
Sbjct: 148 LLDSGNLLLLDHANVTLWQSFDVPTDTILVGQRLPVRNPLFPATTEDD----DISFRLLL 207

Query: 326 TDDDLLL 332
           TDDDLLL
Sbjct: 208 TDDDLLL 209

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BLAST of Lag0001068 vs. NCBI nr
Match: XP_022924990.1 (G-type lectin S-receptor-like serine/threonine-protein kinase At5g35370 [Cucurbita moschata])

HSP 1 Score: 243.0 bits (619), Expect = 3.5e-60
Identity = 133/187 (71.12%), Postives = 151/187 (80.75%), Query Frame = 0


Query: 146 LLLPDSGVLSFAAPLASHSLFPNFTASNFQFIDVDGAFLSSLNNTFTASITNSNSDSSLY 205
           LL P    LS +APLA H+++PNFTASNF+FID  G FL+SLNNTFTASITNS SDSS Y
Sbjct: 11  LLFPFFTFLS-SAPLAFHAVYPNFTASNFKFIDDSGTFLASLNNTFTASITNSKSDSSNY 70

Query: 206 FFLITHVASNTFIWSANPNNLVSTSSPLTLSVASLS-HYNDSGALIWSTPPLPSLVAAMH 265
           FFLITHV S+T IWSANP N VS SSPLTLS A LS   +DSGAL+WSTPPLPS VAAMH
Sbjct: 71  FFLITHVVSDTIIWSANPYNPVSISSPLTLSPAGLSLSDDDSGALVWSTPPLPSPVAAMH 130

Query: 266 LLDSDNILLLDRANVSLWETFDHPTDTIVAGQRLRVQNPLFPAVTQDDMSVGAAEYRLLV 325
           LLDS N+LLLD ANV+LW++FD PTDTI+ GQRL V+NPLFPA T+DD       +RLL+
Sbjct: 131 LLDSGNLLLLDHANVTLWQSFDVPTDTILVGQRLPVRNPLFPATTEDD----DMSFRLLL 190

Query: 326 TDDDLLL 332
           TDDDLLL
Sbjct: 191 TDDDLLL 192

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BLAST of Lag0001068 vs. NCBI nr
Match: KAG6595717.1 (G-type lectin S-receptor-like serine/threonine-protein kinase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 241.9 bits (616), Expect = 7.9e-60
Identity = 132/187 (70.59%), Postives = 151/187 (80.75%), Query Frame = 0


Query: 146 LLLPDSGVLSFAAPLASHSLFPNFTASNFQFIDVDGAFLSSLNNTFTASITNSNSDSSLY 205
           LL P    LS +APLA H+++PNFTASNF+FID  G FL+SLNNTFTASITNS SDSS +
Sbjct: 36  LLFPFFTFLS-SAPLAFHAVYPNFTASNFKFIDDSGTFLASLNNTFTASITNSKSDSSKF 95

Query: 206 FFLITHVASNTFIWSANPNNLVSTSSPLTLSVASLS-HYNDSGALIWSTPPLPSLVAAMH 265
           FFLITHV S+T IWSANP N VS SSPLTLS A LS   +DSGAL+WSTPPLPS VAAMH
Sbjct: 96  FFLITHVVSDTIIWSANPYNPVSISSPLTLSPAGLSLSDDDSGALVWSTPPLPSPVAAMH 155

Query: 266 LLDSDNILLLDRANVSLWETFDHPTDTIVAGQRLRVQNPLFPAVTQDDMSVGAAEYRLLV 325
           LLDS N+LLLD ANV+LW++FD PTDTI+ GQRL V+NPLFPA T+DD       +RLL+
Sbjct: 156 LLDSGNLLLLDHANVTLWQSFDVPTDTILVGQRLPVRNPLFPATTEDD----DMSFRLLL 215

Query: 326 TDDDLLL 332
           TDDDLLL
Sbjct: 216 TDDDLLL 217

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BLAST of Lag0001068 vs. NCBI nr
Match: XP_023518729.1 (G-type lectin S-receptor-like serine/threonine-protein kinase At5g35370 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 239.6 bits (610), Expect = 3.9e-59
Identity = 132/187 (70.59%), Postives = 151/187 (80.75%), Query Frame = 0


Query: 146 LLLPDSGVLSFAAPLASHSLFPNFTASNFQFIDVDGAFLSSLNNTFTASITNSNSDSSLY 205
           LL P    LS +APLA H+++PNFTASNF+FID  GAFL+SLNNTFTASITNS SDSS Y
Sbjct: 11  LLFPFFTFLS-SAPLAFHAVYPNFTASNFKFIDDSGAFLASLNNTFTASITNSKSDSSHY 70

Query: 206 FFLITHVASNTFIWSANPNNLVSTSSPLTLSVASLSHYND-SGALIWSTPPLPSLVAAMH 265
           FFLITHV S+T IWSANP N VS SSPLTLS A LS  +D SGA++WSTPPLPS VAAMH
Sbjct: 71  FFLITHVVSDTVIWSANPYNPVSISSPLTLSPAGLSLSDDESGAVVWSTPPLPSPVAAMH 130

Query: 266 LLDSDNILLLDRANVSLWETFDHPTDTIVAGQRLRVQNPLFPAVTQDDMSVGAAEYRLLV 325
           LLDS N+LLLD ANV+LW++FD PTDTI+ GQRL V+NPLF A T+DD       +RLL+
Sbjct: 131 LLDSGNLLLLDHANVTLWQSFDVPTDTILVGQRLPVRNPLFSATTEDD----DMSFRLLL 190

Query: 326 TDDDLLL 332
           TDDDLLL
Sbjct: 191 TDDDLLL 192

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BLAST of Lag0001068 vs. NCBI nr
Match: XP_011653741.1 (G-type lectin S-receptor-like serine/threonine-protein kinase At5g35370 [Cucumis sativus] >XP_011653742.1 G-type lectin S-receptor-like serine/threonine-protein kinase At5g35370 [Cucumis sativus] >KAE8649601.1 hypothetical protein Csa_012226 [Cucumis sativus])

HSP 1 Score: 235.3 bits (599), Expect = 7.3e-58
Identity = 127/187 (67.91%), Postives = 149/187 (79.68%), Query Frame = 0


Query: 146 LLLPDSGVLSFAAPLASHSLFPNFTASNFQFIDVDGAFLSSLNNTFTASITNSNSDSSLY 205
           L+LP+   L ++AP+AS S+ PNFTASNFQFIDV GAFL SLNN FTASITNSNS +SLY
Sbjct: 10  LVLPN---LFYSAPIASPSISPNFTASNFQFIDVSGAFLVSLNNLFTASITNSNSHTSLY 69

Query: 206 FFLITHVASNTFIWSANPNNLVSTSSPLTLSVASLSHYNDSGALIWSTPPLPSLVAAMHL 265
           FFLI HV SN+ IWSANPN  VSTSS LTLS   LS  +DSG L+WSTPPL S +A+M L
Sbjct: 70  FFLIIHVQSNSIIWSANPNKPVSTSSLLTLSPTGLSLSDDSGLLVWSTPPLSSPIASMLL 129

Query: 266 LDSDNILLLDRANVSLWETFDHPTDTIVAGQRLRVQNPLFPAVTQD-DMSVGAAEYRLLV 325
           LDS N+LLLD +NVSLWE+F +PTDTIV GQRL V N LFPA   D D+S+G ++YRLL+
Sbjct: 130 LDSGNLLLLDHSNVSLWESFHYPTDTIVVGQRLTVMNSLFPAQPDDHDISIGGSQYRLLL 189

Query: 326 TDDDLLL 332
           T +DLLL
Sbjct: 190 TSNDLLL 193

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BLAST of Lag0001068 vs. ExPASy Swiss-Prot
Match: O65238 (G-type lectin S-receptor-like serine/threonine-protein kinase At5g35370 OS=Arabidopsis thaliana OX=3702 GN=At5g35370 PE=2 SV=2)

HSP 1 Score: 125.2 bits (313), Expect = 1.4e-27
Identity = 75/172 (43.60%), Postives = 109/172 (63.37%), Query Frame = 0


Query: 165 LFPNFTASNFQFIDVD-GAFLSSLNNTFTASITNSNSDSSL--YFFLITHVASNTFIWSA 224
           ++PNFTASN +F+D   GAFL S N+ F A + +   D S   ++F + HV S + IWS+
Sbjct: 30  VYPNFTASNLRFVDSSKGAFLLSRNSIFKAGLFSPGGDDSSTGFYFSVVHVDSGSTIWSS 89

Query: 225 NPNNLVSTSSPLTLSVASLSHYND--SGALIWSTPPLPSLVAAMHLLDSDNILLLDRANV 284
           N ++ VS+S  + L+   +S   D  S   +WSTP L S V ++ L D+ N+LLLD  NV
Sbjct: 90  NRDSPVSSSGTMNLTPQGISVIEDGKSQIPVWSTPVLASPVKSLRLTDAGNLLLLDHLNV 149

Query: 285 SLWETFDHPTDTIVAGQRLRVQNPLFPAVTQDDMSVGAAEYRLLVTDDDLLL 332
           SLWE+FD PTD+IV GQRL++   L  +V++ D S G  +Y+ LV + D L+
Sbjct: 150 SLWESFDFPTDSIVLGQRLKLGMFLSGSVSRSDFSTG--DYKFLVGESDGLM 199

[Close]

BLAST of Lag0001068 vs. ExPASy Swiss-Prot
Match: Q9SBQ8 (Kaempferol 3-O-beta-D-galactosyltransferase OS=Petunia hybrida OX=4102 PE=1 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 1.7e-12
Identity = 34/69 (49.28%), Postives = 51/69 (73.91%), Query Frame = 0


Query: 12 HQVLVLAFPFVSHPTCLLNLIRRLADEVPTVKFSFFNTSASNAAIFKDAGRNHNVFPYSV 71
          + V VLAFPF +H   LL L++RLA+ +P V F+FFNTS SN+++F     ++N+ P+++
Sbjct: 4  YHVAVLAFPFATHAGLLLGLVQRLANALPNVTFTFFNTSKSNSSLF-TTPHDNNIKPFNI 63

Query: 72 GDGLPEGYV 81
           DG+PEGYV
Sbjct: 64 SDGVPEGYV 71

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BLAST of Lag0001068 vs. ExPASy Swiss-Prot
Match: A0A2R6Q8R5 (Anthocyanidin 3-O-galactosyltransferase F3GT1 OS=Actinidia chinensis var. chinensis OX=1590841 GN=F3GT1 PE=1 SV=1)

HSP 1 Score: 71.6 bits (174), Expect = 1.8e-11
Identity = 37/67 (55.22%), Postives = 46/67 (68.66%), Query Frame = 0


Query: 14 VLVLAFPFVSHPTCLLNLIRRLADEVPTVKFSFFNTSASNAAIFKDAGRNHNVFPYSVGD 73
          V VLAFPF +HP  LLNL++RLA E P V FSF +T+ SN ++F       N+ PY V D
Sbjct: 12 VAVLAFPFSTHPGPLLNLVQRLAVEAPDVIFSFISTAKSNESLF-SVPNPENIKPYPVWD 71

Query: 74 GLPEGYV 81
          G+PEGYV
Sbjct: 72 GVPEGYV 77

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BLAST of Lag0001068 vs. ExPASy Swiss-Prot
Match: Q8RWZ5 (G-type lectin S-receptor-like serine/threonine-protein kinase SD2-5 OS=Arabidopsis thaliana OX=3702 GN=SD25 PE=1 SV=1)

HSP 1 Score: 71.6 bits (174), Expect = 1.8e-11
Identity = 53/189 (28.04%), Postives = 84/189 (44.44%), Query Frame = 0


Query: 146 LLLPD---SGVLSFAAPLASHSLFPNFTASNFQFIDVDGAFLSSLNNTFTASITNSNSDS 205
           + LPD   +GV S        S+ P F  S   +I+ DG FL S N+ F      +    
Sbjct: 13  VFLPDPLRAGVASIG------SITPGFGGSQMNYINNDGIFLESNNSAFGFGFVTTQDSV 72

Query: 206 SLYFFLITHVASNTFIWSANPNNLVSTSSPLTLSVASLSHYNDSGALIWSTPPLPSLVAA 265
           +L+   I H +S   IWSAN  + VS S           +    G  +W         + 
Sbjct: 73  TLFTLSIIHKSSTKLIWSANRASPVSNSDKFVFD--DNGNVVMEGTEVWRLDNSGKNASR 132

Query: 266 MHLLDSDNILLLDRANVSLWETFDHPTDTIVAGQRLRVQNPLFPAVTQDDMSVGAAEYRL 325
           + L DS N++++     S+WE+FDHPTDT++  Q  +    L  + +  +M+     Y L
Sbjct: 133 IELRDSGNLVVVSVDGTSIWESFDHPTDTLITNQAFKEGMKLTSSPSSSNMT-----YAL 188

Query: 326 LVTDDDLLL 332
            +   D++L
Sbjct: 193 EIKSGDMVL 188

[Close]

BLAST of Lag0001068 vs. ExPASy Swiss-Prot
Match: Q9LFJ8 (Flavonol 3-O-glucosyltransferase OS=Arabidopsis thaliana OX=3702 GN=UGT78D2 PE=1 SV=1)

HSP 1 Score: 68.6 bits (166), Expect = 1.6e-10
Identity = 35/70 (50.00%), Postives = 46/70 (65.71%), Query Frame = 0


Query: 14 VLVLAFPFVSHPTCLLNLIRRLADEVPTVKFSFFNTSASNAAIFK---DAGRNHNVFPYS 73
          V VLAFPF +H   LL + RRLA   P+  FSFFNT+ SN+++F    +A R  N+  Y 
Sbjct: 13 VAVLAFPFGTHAAPLLTVTRRLASASPSTVFSFFNTAQSNSSLFSSGDEADRPANIRVYD 72

Query: 74 VGDGLPEGYV 81
          + DG+PEGYV
Sbjct: 73 IADGVPEGYV 82

[Close]

BLAST of Lag0001068 vs. ExPASy TrEMBL
Match: A0A6J1HU29 (Receptor-like serine/threonine-protein kinase OS=Cucurbita maxima OX=3661 GN=LOC111466183 PE=3 SV=1)

HSP 1 Score: 245.0 bits (624), Expect = 4.5e-61
Identity = 134/187 (71.66%), Postives = 152/187 (81.28%), Query Frame = 0


Query: 146 LLLPDSGVLSFAAPLASHSLFPNFTASNFQFIDVDGAFLSSLNNTFTASITNSNSDSSLY 205
           LL P    LS +APLA H+++PNFTASNF+FID  GAFL+SLNNTFTASITNS SDSS Y
Sbjct: 28  LLFPFFTFLS-SAPLAFHAVYPNFTASNFKFIDDSGAFLASLNNTFTASITNSKSDSSKY 87

Query: 206 FFLITHVASNTFIWSANPNNLVSTSSPLTLSVASLS-HYNDSGALIWSTPPLPSLVAAMH 265
           FFLITHV S+T IWSANP N VS SSPLTLS A LS   +DSGAL+WSTPPLPS VAAMH
Sbjct: 88  FFLITHVVSDTIIWSANPYNPVSISSPLTLSPAGLSLSDDDSGALVWSTPPLPSPVAAMH 147

Query: 266 LLDSDNILLLDRANVSLWETFDHPTDTIVAGQRLRVQNPLFPAVTQDDMSVGAAEYRLLV 325
           LLDS N+LLLD ANV+LW++FD PTDTI+ GQRL V+NPLFPA T+DD       +RLL+
Sbjct: 148 LLDSGNLLLLDHANVTLWQSFDVPTDTILVGQRLPVRNPLFPATTEDD----DISFRLLL 207

Query: 326 TDDDLLL 332
           TDDDLLL
Sbjct: 208 TDDDLLL 209

[Close]

BLAST of Lag0001068 vs. ExPASy TrEMBL
Match: A0A6J1EGM2 (Receptor-like serine/threonine-protein kinase OS=Cucurbita moschata OX=3662 GN=LOC111432373 PE=3 SV=1)

HSP 1 Score: 243.0 bits (619), Expect = 1.7e-60
Identity = 133/187 (71.12%), Postives = 151/187 (80.75%), Query Frame = 0


Query: 146 LLLPDSGVLSFAAPLASHSLFPNFTASNFQFIDVDGAFLSSLNNTFTASITNSNSDSSLY 205
           LL P    LS +APLA H+++PNFTASNF+FID  G FL+SLNNTFTASITNS SDSS Y
Sbjct: 11  LLFPFFTFLS-SAPLAFHAVYPNFTASNFKFIDDSGTFLASLNNTFTASITNSKSDSSNY 70

Query: 206 FFLITHVASNTFIWSANPNNLVSTSSPLTLSVASLS-HYNDSGALIWSTPPLPSLVAAMH 265
           FFLITHV S+T IWSANP N VS SSPLTLS A LS   +DSGAL+WSTPPLPS VAAMH
Sbjct: 71  FFLITHVVSDTIIWSANPYNPVSISSPLTLSPAGLSLSDDDSGALVWSTPPLPSPVAAMH 130

Query: 266 LLDSDNILLLDRANVSLWETFDHPTDTIVAGQRLRVQNPLFPAVTQDDMSVGAAEYRLLV 325
           LLDS N+LLLD ANV+LW++FD PTDTI+ GQRL V+NPLFPA T+DD       +RLL+
Sbjct: 131 LLDSGNLLLLDHANVTLWQSFDVPTDTILVGQRLPVRNPLFPATTEDD----DMSFRLLL 190

Query: 326 TDDDLLL 332
           TDDDLLL
Sbjct: 191 TDDDLLL 192

[Close]

BLAST of Lag0001068 vs. ExPASy TrEMBL
Match: A0A1S4DXP7 (LOW QUALITY PROTEIN: G-type lectin S-receptor-like serine/threonine-protein kinase At5g35370 OS=Cucumis melo OX=3656 GN=LOC103491290 PE=4 SV=1)

HSP 1 Score: 204.1 bits (518), Expect = 8.8e-49
Identity = 116/179 (64.80%), Postives = 135/179 (75.42%), Query Frame = 0


Query: 154 LSFAAPLASHSLFPNFTASNFQFIDVDGAFLSSLNNTFTASITNSNSDSSLYFFLITHVA 213
           L ++AP+AS S+ PNFTASNFQFIDV GAFL SLNN FTA ITNSNS +SLY+FLI HV 
Sbjct: 16  LIYSAPIASPSISPNFTASNFQFIDVPGAFLVSLNNLFTAPITNSNSHTSLYYFLIIHVQ 75

Query: 214 SNTFIWSANPNNLVSTSSPLTLSVASLSHYNDSGALIWSTPPLPSLVAAMHLLDSDNILL 273
           SN+ I SANPN  +STSS LTLS       N+SG L+ STPPL S V +M LLDS N+LL
Sbjct: 76  SNSLICSANPNKPISTSSLLTLS-------NNSGLLVCSTPPLSSPVTSM-LLDSGNLLL 135

Query: 274 LDRANVSLWETFDHPTDTIVAGQRLRVQNPLFPAVTQD-DMSVGAAEYRLLVTDDDLLL 332
           LD +NVS WE+F  P+DTIV GQRL V NPLFPA   D D+S+G ++YRLL T  DLLL
Sbjct: 136 LDHSNVSFWESFHFPSDTIVVGQRLTVMNPLFPAQPDDHDISIGGSQYRLLPTSYDLLL 186

[Close]

BLAST of Lag0001068 vs. ExPASy TrEMBL
Match: A0A5N6QER9 (Receptor-like serine/threonine-protein kinase OS=Carpinus fangiana OX=176857 GN=FH972_002231 PE=3 SV=1)

HSP 1 Score: 180.6 bits (457), Expect = 1.0e-41
Identity = 96/177 (54.24%), Postives = 128/177 (72.32%), Query Frame = 0


Query: 155 SFAAPLASHSLFPNFTASNFQFIDVDGAFLSSLNNTFTASITNSNSDSSLYFFLITHVAS 214
           +F+A + +HS+ PNFTASNF+FID  G FL S N TF ASI+ +N   S Y+F +THVAS
Sbjct: 51  AFSAAIFTHSVSPNFTASNFRFIDNAGTFLLSQNGTFKASISGTNIQPSSYYFSVTHVAS 110

Query: 215 NTFIWSANPNNLVSTSSPLTLSVASLSHYNDSGALIWSTPPLPSLVAAMHLLDSDNILLL 274
           N+ IWSAN N  +S S  L+L+V  LS  N +G L+WSTPPL   VAAM LL++ N++L+
Sbjct: 111 NSIIWSANRNAAMSDSDELSLTVNGLSVANQAGKLLWSTPPLNYKVAAMQLLETGNLVLV 170

Query: 275 DRANVSLWETFDHPTDTIVAGQRLRVQNPLFPAVTQDDMSVGAAEYRLLVTDDDLLL 332
           D  NV++WE+F++PTDTIV GQR+     L  AV++ DMSVG  +YRL VTD D++L
Sbjct: 171 DARNVTMWESFEYPTDTIVVGQRIPAGKSLQSAVSEADMSVG--DYRLEVTDGDVVL 225

[Close]

BLAST of Lag0001068 vs. ExPASy TrEMBL
Match: A0A2I4GE70 (Receptor-like serine/threonine-protein kinase OS=Juglans regia OX=51240 GN=LOC109007109 PE=3 SV=2)

HSP 1 Score: 175.6 bits (444), Expect = 3.3e-40
Identity = 96/178 (53.93%), Postives = 126/178 (70.79%), Query Frame = 0


Query: 156 FAAPLASHSLFPNFTASNFQFIDVDGAFLSSLNNTFTASI--TNSNSDSSLYFFLITHVA 215
           F+ PL++HS+ P F AS F FID  G FL S N TF ASI  T + +  S Y+F ITHVA
Sbjct: 28  FSGPLSTHSIRPPFIASYFLFIDQSGTFLVSPNGTFKASISGTKAQTSPSRYYFSITHVA 87

Query: 216 SNTFIWSANPNNLVSTSSPLTLSVASLSHYNDSGALIWSTPPLPSLVAAMHLLDSDNILL 275
           S+  +WSAN N  +S S  L+LSV  L   N++G L+WSTPPL S +AAM LL++ N+LL
Sbjct: 88  SDAIVWSANRNASMSNSDKLSLSVDGLRVANEAGKLLWSTPPLNSEIAAMQLLETGNLLL 147

Query: 276 LDRANVSLWETFDHPTDTIVAGQRLRVQNPLFPAVTQDDMSVGAAEYRLLVTDDDLLL 332
           +D  N++LWE+F++PTD+IV GQRLRV   L  A+++ D SVG  +YRLL+TD DLLL
Sbjct: 148 VDARNITLWESFENPTDSIVVGQRLRVGKSLESALSEQDQSVG--DYRLLMTDGDLLL 203

[Close]

BLAST of Lag0001068 vs. TAIR 10
Match: AT5G35370.1 (S-locus lectin protein kinase family protein )

HSP 1 Score: 125.2 bits (313), Expect = 1.0e-28
Identity = 75/172 (43.60%), Postives = 109/172 (63.37%), Query Frame = 0


Query: 165 LFPNFTASNFQFIDVD-GAFLSSLNNTFTASITNSNSDSSL--YFFLITHVASNTFIWSA 224
           ++PNFTASN +F+D   GAFL S N+ F A + +   D S   ++F + HV S + IWS+
Sbjct: 30  VYPNFTASNLRFVDSSKGAFLLSRNSIFKAGLFSPGGDDSSTGFYFSVVHVDSGSTIWSS 89

Query: 225 NPNNLVSTSSPLTLSVASLSHYND--SGALIWSTPPLPSLVAAMHLLDSDNILLLDRANV 284
           N ++ VS+S  + L+   +S   D  S   +WSTP L S V ++ L D+ N+LLLD  NV
Sbjct: 90  NRDSPVSSSGTMNLTPQGISVIEDGKSQIPVWSTPVLASPVKSLRLTDAGNLLLLDHLNV 149

Query: 285 SLWETFDHPTDTIVAGQRLRVQNPLFPAVTQDDMSVGAAEYRLLVTDDDLLL 332
           SLWE+FD PTD+IV GQRL++   L  +V++ D S G  +Y+ LV + D L+
Sbjct: 150 SLWESFDFPTDSIVLGQRLKLGMFLSGSVSRSDFSTG--DYKFLVGESDGLM 199

[Close]

BLAST of Lag0001068 vs. TAIR 10
Match: AT4G32300.1 (S-domain-2 5 )

HSP 1 Score: 71.6 bits (174), Expect = 1.3e-12
Identity = 53/189 (28.04%), Postives = 84/189 (44.44%), Query Frame = 0


Query: 146 LLLPD---SGVLSFAAPLASHSLFPNFTASNFQFIDVDGAFLSSLNNTFTASITNSNSDS 205
           + LPD   +GV S        S+ P F  S   +I+ DG FL S N+ F      +    
Sbjct: 13  VFLPDPLRAGVASIG------SITPGFGGSQMNYINNDGIFLESNNSAFGFGFVTTQDSV 72

Query: 206 SLYFFLITHVASNTFIWSANPNNLVSTSSPLTLSVASLSHYNDSGALIWSTPPLPSLVAA 265
           +L+   I H +S   IWSAN  + VS S           +    G  +W         + 
Sbjct: 73  TLFTLSIIHKSSTKLIWSANRASPVSNSDKFVFD--DNGNVVMEGTEVWRLDNSGKNASR 132

Query: 266 MHLLDSDNILLLDRANVSLWETFDHPTDTIVAGQRLRVQNPLFPAVTQDDMSVGAAEYRL 325
           + L DS N++++     S+WE+FDHPTDT++  Q  +    L  + +  +M+     Y L
Sbjct: 133 IELRDSGNLVVVSVDGTSIWESFDHPTDTLITNQAFKEGMKLTSSPSSSNMT-----YAL 188

Query: 326 LVTDDDLLL 332
            +   D++L
Sbjct: 193 EIKSGDMVL 188

[Close]

BLAST of Lag0001068 vs. TAIR 10
Match: AT5G17050.1 (UDP-glucosyl transferase 78D2 )

HSP 1 Score: 68.6 bits (166), Expect = 1.1e-11
Identity = 35/70 (50.00%), Postives = 46/70 (65.71%), Query Frame = 0


Query: 14 VLVLAFPFVSHPTCLLNLIRRLADEVPTVKFSFFNTSASNAAIFK---DAGRNHNVFPYS 73
          V VLAFPF +H   LL + RRLA   P+  FSFFNT+ SN+++F    +A R  N+  Y 
Sbjct: 13 VAVLAFPFGTHAAPLLTVTRRLASASPSTVFSFFNTAQSNSSLFSSGDEADRPANIRVYD 72

Query: 74 VGDGLPEGYV 81
          + DG+PEGYV
Sbjct: 73 IADGVPEGYV 82

[Close]

BLAST of Lag0001068 vs. TAIR 10
Match: AT5G17040.1 (UDP-Glycosyltransferase superfamily protein )

HSP 1 Score: 58.9 bits (141), Expect = 8.8e-09
Identity = 32/67 (47.76%), Postives = 41/67 (61.19%), Query Frame = 0


Query: 14 VLVLAFPFVSHPTCLLNLIRRLADEVPTVKFSFFNTSASNAAIFKDAGRNHNVFPYSVGD 73
          V VLAFPF SH   +L + RRLA   P+  FSF NTS SN ++   +    N+  + V D
Sbjct: 6  VAVLAFPFGSHGQAILAVTRRLATAAPSTVFSFLNTSQSNFSLL-SSDLPPNIRVHDVSD 65

Query: 74 GLPEGYV 81
          G+PEGYV
Sbjct: 66 GVPEGYV 71

[Close]

BLAST of Lag0001068 vs. TAIR 10
Match: AT1G78830.1 (Curculin-like (mannose-binding) lectin family protein )

HSP 1 Score: 55.1 bits (131), Expect = 1.3e-07
Identity = 41/132 (31.06%), Postives = 63/132 (47.73%), Query Frame = 0


Query: 178 DVDGAFLSSLNNTFTAS---ITNSNSDSSLYFF-----LITHVASNTFIWSANPNNLVST 237
           D    F+ S N +F  S   +   N+  S Y       L    ++  +IW AN NN V  
Sbjct: 46  DASYRFIESSNQSFFTSPFQLLFYNTTPSAYILALRVGLRRDESTMRWIWDANRNNPVGE 105

Query: 238 SSPLTLS-VASLSHYNDSGALIWSTPPLPSLVAAMHLLDSDNILLLDRANVSLWETFDHP 297
           ++ L+L    +L      G + W T      V    +L + NI+L D+    +W++FDHP
Sbjct: 106 NATLSLGRNGNLVLAEADGRVKWQTNTANKGVTGFQILPNGNIVLHDKNGKFVWQSFDHP 165

Query: 298 TDTIVAGQRLRV 301
           TDT++ GQ L+V
Sbjct: 166 TDTLLTGQSLKV 177

The following BLAST results are available for this feature:

BLAST of Lag0001068 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022966534.1	9.3e-61	71.66	G-type lectin S-receptor-like serine/threonine-protein kinase At5g35370 [Cucurbi...	[more]
XP_022924990.1	3.5e-60	71.12	G-type lectin S-receptor-like serine/threonine-protein kinase At5g35370 [Cucurbi...	[more]
KAG6595717.1	7.9e-60	70.59	G-type lectin S-receptor-like serine/threonine-protein kinase, partial [Cucurbit...	[more]
XP_023518729.1	3.9e-59	70.59	G-type lectin S-receptor-like serine/threonine-protein kinase At5g35370 [Cucurbi...	[more]
XP_011653741.1	7.3e-58	67.91	G-type lectin S-receptor-like serine/threonine-protein kinase At5g35370 [Cucumis...	[more]

BLAST of Lag0001068 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O65238	1.4e-27	43.60	G-type lectin S-receptor-like serine/threonine-protein kinase At5g35370 OS=Arabi...	[more]
Q9SBQ8	1.7e-12	49.28	Kaempferol 3-O-beta-D-galactosyltransferase OS=Petunia hybrida OX=4102 PE=1 SV=1	[more]
A0A2R6Q8R5	1.8e-11	55.22	Anthocyanidin 3-O-galactosyltransferase F3GT1 OS=Actinidia chinensis var. chinen...	[more]
Q8RWZ5	1.8e-11	28.04	G-type lectin S-receptor-like serine/threonine-protein kinase SD2-5 OS=Arabidops...	[more]
Q9LFJ8	1.6e-10	50.00	Flavonol 3-O-glucosyltransferase OS=Arabidopsis thaliana OX=3702 GN=UGT78D2 PE=1...	[more]

BLAST of Lag0001068 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HU29	4.5e-61	71.66	Receptor-like serine/threonine-protein kinase OS=Cucurbita maxima OX=3661 GN=LOC...	[more]
A0A6J1EGM2	1.7e-60	71.12	Receptor-like serine/threonine-protein kinase OS=Cucurbita moschata OX=3662 GN=L...	[more]
A0A1S4DXP7	8.8e-49	64.80	LOW QUALITY PROTEIN: G-type lectin S-receptor-like serine/threonine-protein kina...	[more]
A0A5N6QER9	1.0e-41	54.24	Receptor-like serine/threonine-protein kinase OS=Carpinus fangiana OX=176857 GN=...	[more]
A0A2I4GE70	3.3e-40	53.93	Receptor-like serine/threonine-protein kinase OS=Juglans regia OX=51240 GN=LOC10...	[more]

BLAST of Lag0001068 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G35370.1	1.0e-28	43.60	S-locus lectin protein kinase family protein	[more]
AT4G32300.1	1.3e-12	28.04	S-domain-2 5	[more]
AT5G17050.1	1.1e-11	50.00	UDP-glucosyl transferase 78D2	[more]
AT5G17040.1	8.8e-09	47.76	UDP-Glycosyltransferase superfamily protein	[more]
AT1G78830.1	1.3e-07	31.06	Curculin-like (mannose-binding) lectin family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001480	Bulb-type lectin domain	SMART	SM00108	blect_4	coord: 175..288 e-value: 1.0E-4 score: 31.7
IPR001480	Bulb-type lectin domain	PFAM	PF01453	B_lectin	coord: 216..301 e-value: 2.7E-10 score: 40.7
IPR001480	Bulb-type lectin domain	PROSITE	PS50927	BULB_LECTIN	coord: 168..286 score: 8.348673
None	No IPR available	GENE3D	2.90.10.30		coord: 179..321 e-value: 2.3E-6 score: 29.6
None	No IPR available	GENE3D	3.40.50.2000	Glycogen Phosphorylase B;	coord: 7..134 e-value: 8.8E-10 score: 40.4
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 86..138
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 109..138
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 86..108
None	No IPR available	PANTHER	PTHR27009	RUST RESISTANCE KINASE LR10-RELATED	coord: 154..331
None	No IPR available	PANTHER	PTHR27009:SF250	SUBFAMILY NOT NAMED	coord: 154..331
None	No IPR available	SUPERFAMILY	53756	UDP-Glycosyltransferase/glycogen phosphorylase	coord: 12..107
IPR036426	Bulb-type lectin domain superfamily	SUPERFAMILY	51110	alpha-D-mannose-specific plant lectins	coord: 205..290

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0001068.1	Lag0001068.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006468	protein phosphorylation
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0030246	carbohydrate binding
molecular_function	GO:0106310	protein serine kinase activity
molecular_function	GO:0004674	protein serine/threonine kinase activity
molecular_function	GO:0004712	protein serine/threonine/tyrosine kinase activity