Lag0000775 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0000775
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Peptide chain release factor
Location	chr4: 15692598 .. 15696825 (+)
RNA-Seq Expression	Lag0000775
Synteny	Lag0000775

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGTCATCTACCGAAGGAGATTTTACAGAAGAGAAACGAAGAGGAAGGCATGGAGGTTGTTCAGCGCCCATTGTGGCTCTGCAGCTCGCCCGTTTTTCCACTTCCCAAATTTCAAACCACTGCAACTTCGATCTCCCTACAATTTCCACTGCGCCCATCTTCCCCGTCTCCCTACTTCCGCACTTCTCTCTCAATTCACTCTCGTTTTTCTCACGGTGAGTCGATTCAATGTCATACCCAATTGCTTTCAGTTTCTGTAACTTGTTGGTTTCTTCCTGCAGTTCCGTTTGCAACACCCGAAAGCCAAGTGGGTGAGGAGCTCGATGCTGATACGCGAGAATGGGCGATGCAAGGTATGTTTACTTCTCCTACAAAACAATTAGTTCATAGACTCAAACTACAGTGTTCATGGTGTTCTGTTCTAACTATTGAACAGCCTTAGTTGTCTTTATGGGTCCTCGAATGACTGGTAAACCATGTTGATAGAGGGTGACATAACAATAAGTTACTCGCTAGTTGTATCTCATTCATCAAATGGGGTTAATGTTTATGTTACACAGTGTTTAATTTCAAATGCTGCTATATGAATCTTCACATTCACTTGAATGATAAATTGCTTAAGCCTTTTTATTATTTTTGTTCTGGTAAGCCGGAAGCTAATAAATTACATCCTTAAAATATTATAATATCTATTGCGACTGTTTTTAAGAGCCAACAGGCTGTTTATTGTCCCAGGTTTTGATTTTACACATCAATTGTTCCTTCATAGAATCTACTACTTTTTTGGCCCTTTATTGGAGTAGACACACCCCCTTTCTGTAATTACTCCCTCTCTTCCCTTTTATCCCATTGGAGATGTCTTTTGTAATTACCTATGATAGGTTGTTCTTTTGTATCTATTTCATACATCAATGAAATGGTTTATGTTTCCTATCCAAAAAAAAAAAAAAAATTGTATCAATGGACGCATCTAATTCCCATTTTTCCCTTTTGGATCCAGTGGTGTTTGCAACACAATGGAAATGATATAACCTGTGCTTGTTGTTGCTGACAGATTTTTATTCATTAAGGAAAGAAGTGGAAACAACCTCAGAGCGTGTGGAAGAGATAAGAAACTCTGCCGGTTTGTTACAGTTGGATCAAGAACTTGCAGATTTGGAGTCGAAGGCAGCTGATGACTCTTTCTGGGATGACAGATCTAAGGCTCAACAAGTTCTCGTGGCCCTAACTGATGTGAAAGATAAAATAAAGATGCTTACTGATTTCAAAACACAGGTAATGTATATAGCTTGCAATCTAATATTTAGAGAATACCCTTCTTTTTTTCTTTTTTCTTTCAAATTTTTCATTGAAGTAATAACAAGAGACTAATGCTAAAAGATACAAGGCCCAAAAGGGAAAAAGATTAAAAAGAAAAACAACATAAACTCAGTAAGAAATAAAAACTTCCCAATTTATACAAATATCTTGTAGAGAAAAATTCAGAAATAGTTTAGAAACAGAACACCATTGAGAAGCTTTCAGTCTTCCTTGCTCAAATCAATCAAATCAAGGTTTTTGAACATTCTCAAAAACTCGCTGATTATGCTCTAGCCAAATTTCAGAAACAATAGCTACACTGTATTAGACCATAACAAATTGAGCTTTTGGAGGCAAGAAAGCCCAAGAGTGTTGAGCTTAGAGTTATGGGAGAAAATCTATTTCAAATTAAAATTTGAAATAATTGAAACCAACAACTCATGACTAATGGAACAACCATGGGCTGGCATAGTGGTTAATAAGAGCTATGTATAAAAAAGGACTTAGAGGGATATGTTCAAGCCATGGTGACCACCTACCTAAAATTTAATATCCTATCGAGTTACATTAATAATCAAATATAGTAGGGTTAGACGGTTGCCCCGTGAGGATGGTCAAGGTGCTTGCAAATTGTCCTGGATAATCATAGATAAAAAATTAAAAAAGAAAAAAACAAACTCATGCCTAATGGACAATCGAAGAATAGGTGGTTGAGTGTCTCAGCATCCCCATTTACTAAAGTCACATATATGGACATATATATATGAGTGTCTCAGCATCCCCATTTTCTAAAGTCACATATATAGACATATATGGGAGCCCTATAATGAAGAGTATAAAATTATAGTAAAGGATAAATGACGAATAAAATCTTGAATGGAGATATGTAGATGAATATACATAATATGAAATAGTCTACTCAGGGTAGAGCGTCCATGTTAGTCATGCTGTGAGACCTCCTATTGTTTACATGTACTCGAGAAAGGCTAAAATGGGGAAACCATGAAAAACGGTTAGAGTTGGGACAAGAAAGGGATCTTGGGATCATGCTCTGTGTACGGTCTGAGGAGGAAGTGTTTTTGTAGGGGTTTGAGGGGTCGGTGTCTGGGTATGGAATTTGGGCCAATAATCTGACCAAGCTTTTGAAAAGTGATTGAAATCATCTCCTTAGGTGTGGGTTGTGTTATGATGATTCAGGTTGAAGAAGCAGAAACAATAGTTAACCTGACTGAAGAGATGGATTCTGTAGATACTGGGCTTCTTGAAGAGGCTGCTAGTATCATTAAAGACCTGAACAAGGCACTGGATCAATTTGAATTAACTCAACTTCTTTCTGGTCCGTATGACAAAGAAGGCGCTGTCATCTCTATCTCGGCTGGAGCTGGGGGTACTGATGCACAGGTAAATAGTTAAGGGCTTATCTTGGAACCTGTTTTCAAAGTATTGTTTACAAAACTGCTTCTAAGAATTATAGTCAGGTGTGTAATTTTGAACTTTAGTTTTTTCATGAAATTTATTGCTTCTCCGATGTTAAATTTTTGATAATCTGCAGTGCAGGATTGGGCTGACATGCTTCTGAGGATGTATGTAAGATGGGGAGAGAAACAAAGATACAAAACAAGAGTGGTTGAAAAATCTGCTGGAGAGGAAGCTGGAATAAAATCAGCTACAATTGAAATTGAAGGCCGTTATGCTTACGGATATATATCCGGGGAGAAAGGAACGCACAGGATAGTGCGACAGTCTCCTTTTAACTCTAAAGGACTTCGACAGGTAATTTTAACACTACCCCCTACATCGCTAGACGCTAGCGCTTGGAGATATTAAGAAAAGATAGACAATTGAAGGCACCTAAAGCCATCATGTTTTACATTTCTAATGAACTGAAAATTAGAATCCAAAATCATTATTCATTGACTTCTAATGAATGATGTGAAACTAAATGACCGCCTATCCAGACAAGCTTTTCTGGTGTGGAAGTGATGCCTCTCCTTCCCGAAGAGTCCATGGATGTTGAACTGCCAGAAGAGGACCTGGAAATAAGTTTTTCAAGGGCAGGCGGAAAAGGAGGTCAAAATGTTAACAAGGTTGAAACGGCTGTACGCATCACTCATATCCCCACAGGCGTCACGGTTCGCTGCACAGGTTTTCCATCCTAATTTAACTTTTAGTAAGATTTAATAGGCCACTGTCCCCAACTTTTACCAAAATTGTTCAAAGAGATCATCACTTGTAGCCAAATCATTTCATTTTAAGTTCTCACCAAATCAATTGATTGACGTGTAACATTATCATGTTTGAGTATTGATTTGCTTTGAGAATACATTTAATCCAAATAGTTTTTTGAAATGTTTAGCATTTGCATGTTGTCGTTCAAATCTCCCTGTTTGAAGTCTTCCATTTAGGATTGATGTTGGCAAGTGGATCAGATGTGTTTGCATTTGACAAACAAACCTTTCAGACTTTCCCATGTTGCATGGTTTTAATAATTCAACCTGGCTGGTGACAAGTTTTGATTGAATCAGATATTTCTTCCCAAATTCCATTCTATATTTCTTTACTACAAATTATGAATAATCTCTGAACTAATGCCATTTCGTTTTTTTCTTGACTGTAATGCAGAGGAGAGATCACAGCTAGCAAACAAAATCAAGGCACTAAGCAGGCTGAAAGCCAAGCTGTTAGTCATAGCCGAGGAGCAACGTGCATCTGAAATCAAGCAAATACGAGGAGATGCCGTAAAGGCCGAGTGGGGGCAGCAAATAAGGAATTATGTGTTTCATCCATACAAGCTTGTGAAAGATGTTAGGACAGGATGTGAGACATCAGACATTATCTCGGTAATGGATGGTGAATTGGAGCCCTTCATCAAAGCTTATCTGAAATACAAATACAATATGGCATTATCTACTGGTGAGGTTAACTGA

mRNA sequence

ATGGGTCATCTACCGAAGGAGATTTTACAGAAGAGAAACGAAGAGGAAGGCATGGAGGTTGTTCAGCGCCCATTGTGGCTCTGCAGCTCGCCCGTTTTTCCACTTCCCAAATTTCAAACCACTGCAACTTCGATCTCCCTACAATTTCCACTGCGCCCATCTTCCCCGTCTCCCTACTTCCGCACTTCTCTCTCAATTCACTCTCGTTTTTCTCACGTTCCGTTTGCAACACCCGAAAGCCAAGTGGGTGAGGAGCTCGATGCTGATACGCGAGAATGGGCGATGCAAGATTTTTATTCATTAAGGAAAGAAGTGGAAACAACCTCAGAGCGTGTGGAAGAGATAAGAAACTCTGCCGGTTTGTTACAGTTGGATCAAGAACTTGCAGATTTGGAGTCGAAGGCAGCTGATGACTCTTTCTGGGATGACAGATCTAAGGCTCAACAAGTTCTCGTGGCCCTAACTGATGTGAAAGATAAAATAAAGATGCTTACTGATTTCAAAACACAGGTTGAAGAAGCAGAAACAATAGTTAACCTGACTGAAGAGATGGATTCTGTAGATACTGGGCTTCTTGAAGAGGCTGCTAGTATCATTAAAGACCTGAACAAGGCACTGGATCAATTTGAATTAACTCAACTTCTTTCTGGTCCGTATGACAAAGAAGGCGCTGTCATCTCTATCTCGGCTGGAGCTGGGGGTACTGATGCACAGGATTGGGCTGACATGCTTCTGAGGATGTATGTAAGATGGGGAGAGAAACAAAGATACAAAACAAGAGTGGTTGAAAAATCTGCTGGAGAGGAAGCTGGAATAAAATCAGCTACAATTGAAATTGAAGGCCGTTATGCTTACGGATATATATCCGGGGAGAAAGGAACGCACAGGATAGTGCGACAGTCTCCTTTTAACTCTAAAGGACTTCGACAGACAAGCTTTTCTGGTGTGGAAGTGATGCCTCTCCTTCCCGAAGAGTCCATGGATGTTGAACTGCCAGAAGAGGACCTGGAAATAAGTTTTTCAAGGGCAGGCGGAAAAGGAGGTCAAAATGTTAACAAGGTTGAAACGGCTGTACGCATCACTCATATCCCCACAGGCGTCACGGTTCGCTGCACAGAGGAGAGATCACAGCTAGCAAACAAAATCAAGGCACTAAGCAGGCTGAAAGCCAAGCTGTTAGTCATAGCCGAGGAGCAACGTGCATCTGAAATCAAGCAAATACGAGGAGATGCCGTAAAGGCCGAGTGGGGGCAGCAAATAAGGAATTATGTGTTTCATCCATACAAGCTTGTGAAAGATGTTAGGACAGGATGTGAGACATCAGACATTATCTCGGTAATGGATGGTGAATTGGAGCCCTTCATCAAAGCTTATCTGAAATACAAATACAATATGGCATTATCTACTGGTGAGGTTAACTGA

Coding sequence (CDS)

ATGGGTCATCTACCGAAGGAGATTTTACAGAAGAGAAACGAAGAGGAAGGCATGGAGGTTGTTCAGCGCCCATTGTGGCTCTGCAGCTCGCCCGTTTTTCCACTTCCCAAATTTCAAACCACTGCAACTTCGATCTCCCTACAATTTCCACTGCGCCCATCTTCCCCGTCTCCCTACTTCCGCACTTCTCTCTCAATTCACTCTCGTTTTTCTCACGTTCCGTTTGCAACACCCGAAAGCCAAGTGGGTGAGGAGCTCGATGCTGATACGCGAGAATGGGCGATGCAAGATTTTTATTCATTAAGGAAAGAAGTGGAAACAACCTCAGAGCGTGTGGAAGAGATAAGAAACTCTGCCGGTTTGTTACAGTTGGATCAAGAACTTGCAGATTTGGAGTCGAAGGCAGCTGATGACTCTTTCTGGGATGACAGATCTAAGGCTCAACAAGTTCTCGTGGCCCTAACTGATGTGAAAGATAAAATAAAGATGCTTACTGATTTCAAAACACAGGTTGAAGAAGCAGAAACAATAGTTAACCTGACTGAAGAGATGGATTCTGTAGATACTGGGCTTCTTGAAGAGGCTGCTAGTATCATTAAAGACCTGAACAAGGCACTGGATCAATTTGAATTAACTCAACTTCTTTCTGGTCCGTATGACAAAGAAGGCGCTGTCATCTCTATCTCGGCTGGAGCTGGGGGTACTGATGCACAGGATTGGGCTGACATGCTTCTGAGGATGTATGTAAGATGGGGAGAGAAACAAAGATACAAAACAAGAGTGGTTGAAAAATCTGCTGGAGAGGAAGCTGGAATAAAATCAGCTACAATTGAAATTGAAGGCCGTTATGCTTACGGATATATATCCGGGGAGAAAGGAACGCACAGGATAGTGCGACAGTCTCCTTTTAACTCTAAAGGACTTCGACAGACAAGCTTTTCTGGTGTGGAAGTGATGCCTCTCCTTCCCGAAGAGTCCATGGATGTTGAACTGCCAGAAGAGGACCTGGAAATAAGTTTTTCAAGGGCAGGCGGAAAAGGAGGTCAAAATGTTAACAAGGTTGAAACGGCTGTACGCATCACTCATATCCCCACAGGCGTCACGGTTCGCTGCACAGAGGAGAGATCACAGCTAGCAAACAAAATCAAGGCACTAAGCAGGCTGAAAGCCAAGCTGTTAGTCATAGCCGAGGAGCAACGTGCATCTGAAATCAAGCAAATACGAGGAGATGCCGTAAAGGCCGAGTGGGGGCAGCAAATAAGGAATTATGTGTTTCATCCATACAAGCTTGTGAAAGATGTTAGGACAGGATGTGAGACATCAGACATTATCTCGGTAATGGATGGTGAATTGGAGCCCTTCATCAAAGCTTATCTGAAATACAAATACAATATGGCATTATCTACTGGTGAGGTTAACTGA

Protein sequence

MGHLPKEILQKRNEEEGMEVVQRPLWLCSSPVFPLPKFQTTATSISLQFPLRPSSPSPYFRTSLSIHSRFSHVPFATPESQVGEELDADTREWAMQDFYSLRKEVETTSERVEEIRNSAGLLQLDQELADLESKAADDSFWDDRSKAQQVLVALTDVKDKIKMLTDFKTQVEEAETIVNLTEEMDSVDTGLLEEAASIIKDLNKALDQFELTQLLSGPYDKEGAVISISAGAGGTDAQDWADMLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKSATIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPEESMDVELPEEDLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTGCETSDIISVMDGELEPFIKAYLKYKYNMALSTGEVN

Homology

[Close]

BLAST of Lag0000775 vs. NCBI nr
Match: XP_022148293.1 (peptide chain release factor PrfB1, chloroplastic [Momordica charantia])

HSP 1 Score: 821.6 bits (2121), Expect = 3.4e-234
Identity = 429/458 (93.67%), Postives = 439/458 (95.85%), Query Frame = 0


Query: 18  MEVVQRPLWLCS--SPVFPLPKFQTTATSISLQFPLRPSSPSPYFRTSLSIHSRFSHVPF 77
           M V+ RPLWLC   S VFP PKFQ+T TS+SL+FP  PSSPSP+F TSLSI SRFS V F
Sbjct: 1   MAVLLRPLWLCGSFSLVFPFPKFQSTRTSLSLEFPPPPSSPSPHFPTSLSIRSRFSIVSF 60

Query: 78  ATPESQVGEELDADTREWAMQDFYSLRKEVETTSERVEEIRNSAGLLQLDQELADLESKA 137
           ATPESQVGEELDADTREWAMQDFYSLRKEVETTSERVEEIRNSAGL QLDQELADLESKA
Sbjct: 61  ATPESQVGEELDADTREWAMQDFYSLRKEVETTSERVEEIRNSAGLFQLDQELADLESKA 120

Query: 138 ADDSFWDDRSKAQQVLVALTDVKDKIKMLTDFKTQVEEAETIVNLTEEMDSVDTGLLEEA 197
           AD++ WDDRSKAQQVLVALTDVKDKIKMLTDFKTQVEEAETIV LTEEMDSVDTGLLEEA
Sbjct: 121 ADNALWDDRSKAQQVLVALTDVKDKIKMLTDFKTQVEEAETIVKLTEEMDSVDTGLLEEA 180

Query: 198 ASIIKDLNKALDQFELTQLLSGPYDKEGAVISISAGAGGTDAQDWADMLLRMYVRWGEKQ 257
           ASIIKDLNKALDQFELTQLLSGPYDKEGAVISISAGAGGTDAQDWADMLLRMYVRWGEKQ
Sbjct: 181 ASIIKDLNKALDQFELTQLLSGPYDKEGAVISISAGAGGTDAQDWADMLLRMYVRWGEKQ 240

Query: 258 RYKTRVVEKSAGEEAGIKSATIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSG 317
           RYKTRVVEKSAGEEAGIKSATIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSG
Sbjct: 241 RYKTRVVEKSAGEEAGIKSATIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSG 300

Query: 318 VEVMPLLPEESMDVELPEEDLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEER 377
           VEVMPLLP+ESMDVELPEEDLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEER
Sbjct: 301 VEVMPLLPDESMDVELPEEDLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEER 360

Query: 378 SQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVR 437
           SQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVR
Sbjct: 361 SQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVR 420

Query: 438 TGCETSDIISVMDGELEPFIKAYLKYKYNMALSTGEVN 474
           TG ETSDI+SVMDGELEPFIKAYLKYKY+MALSTG VN
Sbjct: 421 TGYETSDIVSVMDGELEPFIKAYLKYKYSMALSTGGVN 458

[Close]

BLAST of Lag0000775 vs. NCBI nr
Match: XP_023525877.1 (peptide chain release factor PrfB1, chloroplastic isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 811.6 bits (2095), Expect = 3.5e-231
Identity = 420/456 (92.11%), Postives = 438/456 (96.05%), Query Frame = 0


Query: 18  MEVVQRPLWLCSSPVFPLPKFQTTATSISLQFPLRPSSPSPYFRTSLSIHSRFSHVPFAT 77
           M+VVQRPLWLC+SPVFPLP+ QTT +S  LQFPL        FR+SLSIHSR S VPFAT
Sbjct: 1   MDVVQRPLWLCTSPVFPLPRLQTTRSSSELQFPL------SRFRSSLSIHSRSSLVPFAT 60

Query: 78  PESQVGEELDADTREWAMQDFYSLRKEVETTSERVEEIRNSAGLLQLDQELADLESKAAD 137
           PES VGEE+D+DT+EWAMQDFYSLRKEVET SERVEEIRNSAGLLQLDQE+ADLESKAA+
Sbjct: 61  PESPVGEEVDSDTQEWAMQDFYSLRKEVETASERVEEIRNSAGLLQLDQEVADLESKAAE 120

Query: 138 DSFWDDRSKAQQVLVALTDVKDKIKMLTDFKTQVEEAETIVNLTEEMDSVDTGLLEEAAS 197
           +SFWDDRSKAQQVL+ALTDVKDKIKMLTDFKTQVEEAETIV LTEEMDSVDTGLLEEAAS
Sbjct: 121 NSFWDDRSKAQQVLMALTDVKDKIKMLTDFKTQVEEAETIVKLTEEMDSVDTGLLEEAAS 180

Query: 198 IIKDLNKALDQFELTQLLSGPYDKEGAVISISAGAGGTDAQDWADMLLRMYVRWGEKQRY 257
           IIKDLNKALDQFELTQLLSGPYDKEGAVI+ISAGAGGTDAQDWADMLLRMYVRWGEKQRY
Sbjct: 181 IIKDLNKALDQFELTQLLSGPYDKEGAVITISAGAGGTDAQDWADMLLRMYVRWGEKQRY 240

Query: 258 KTRVVEKSAGEEAGIKSATIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVE 317
           KTRVVEKSAGEEAGIKSATIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVE
Sbjct: 241 KTRVVEKSAGEEAGIKSATIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVE 300

Query: 318 VMPLLPEESMDVELPEEDLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQ 377
           VMPLLP+ESMDVE+PEEDLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQ
Sbjct: 301 VMPLLPDESMDVEIPEEDLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQ 360

Query: 378 LANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTG 437
           LANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTG
Sbjct: 361 LANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTG 420

Query: 438 CETSDIISVMDGELEPFIKAYLKYKYNMALSTGEVN 474
            ETSDI+SVMDGELEPFIKAYLKYKY+MAL+TGEVN
Sbjct: 421 HETSDIVSVMDGELEPFIKAYLKYKYSMALATGEVN 450

[Close]

BLAST of Lag0000775 vs. NCBI nr
Match: XP_022949524.1 (peptide chain release factor PrfB1, chloroplastic isoform X2 [Cucurbita moschata])

HSP 1 Score: 811.2 bits (2094), Expect = 4.6e-231
Identity = 421/456 (92.32%), Postives = 437/456 (95.83%), Query Frame = 0


Query: 18  MEVVQRPLWLCSSPVFPLPKFQTTATSISLQFPLRPSSPSPYFRTSLSIHSRFSHVPFAT 77
           M+VVQRPLWLC+SPVFPLP+ QT  TS  LQFPL        FRTSLSIHSR S V FAT
Sbjct: 1   MDVVQRPLWLCTSPVFPLPRLQTARTSSPLQFPL------SRFRTSLSIHSRSSLVRFAT 60

Query: 78  PESQVGEELDADTREWAMQDFYSLRKEVETTSERVEEIRNSAGLLQLDQELADLESKAAD 137
           PESQVGEE+D+DT+EWAMQDFYSLRKEVET SERVEEIRNSAGLLQLDQE+ADLESKAA+
Sbjct: 61  PESQVGEEVDSDTQEWAMQDFYSLRKEVETASERVEEIRNSAGLLQLDQEVADLESKAAE 120

Query: 138 DSFWDDRSKAQQVLVALTDVKDKIKMLTDFKTQVEEAETIVNLTEEMDSVDTGLLEEAAS 197
           +SFWDDRSKAQQVL+ALTDVKDKIKMLTDFKTQVEEAETIV LTEEMDSVDTGLLEEAAS
Sbjct: 121 NSFWDDRSKAQQVLMALTDVKDKIKMLTDFKTQVEEAETIVKLTEEMDSVDTGLLEEAAS 180

Query: 198 IIKDLNKALDQFELTQLLSGPYDKEGAVISISAGAGGTDAQDWADMLLRMYVRWGEKQRY 257
           IIKDLNKALDQFELTQLLSGPYDKEGAVI+ISAGAGGTDAQDWADMLLRMYVRWGEKQRY
Sbjct: 181 IIKDLNKALDQFELTQLLSGPYDKEGAVITISAGAGGTDAQDWADMLLRMYVRWGEKQRY 240

Query: 258 KTRVVEKSAGEEAGIKSATIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVE 317
           KTRVVEKSAGEEAGIKSATIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVE
Sbjct: 241 KTRVVEKSAGEEAGIKSATIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVE 300

Query: 318 VMPLLPEESMDVELPEEDLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQ 377
           VMPLLP+ESMDVE+PEEDLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQ
Sbjct: 301 VMPLLPDESMDVEIPEEDLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQ 360

Query: 378 LANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTG 437
           LANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTG
Sbjct: 361 LANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTG 420

Query: 438 CETSDIISVMDGELEPFIKAYLKYKYNMALSTGEVN 474
            ETSDI+SVMDGELEPFIKAYLKYKY+MAL+TGEVN
Sbjct: 421 HETSDIVSVMDGELEPFIKAYLKYKYSMALATGEVN 450

[Close]

BLAST of Lag0000775 vs. NCBI nr
Match: XP_022998734.1 (peptide chain release factor PrfB1, chloroplastic isoform X2 [Cucurbita maxima])

HSP 1 Score: 809.3 bits (2089), Expect = 1.8e-230
Identity = 420/456 (92.11%), Postives = 437/456 (95.83%), Query Frame = 0


Query: 18  MEVVQRPLWLCSSPVFPLPKFQTTATSISLQFPLRPSSPSPYFRTSLSIHSRFSHVPFAT 77
           M+VVQRPLWLC+SPVFP P+ QTT TS  LQ+PL        FRTSLSIHSR S V FAT
Sbjct: 1   MDVVQRPLWLCTSPVFPPPRLQTTRTSSPLQYPL------SRFRTSLSIHSRSSLVRFAT 60

Query: 78  PESQVGEELDADTREWAMQDFYSLRKEVETTSERVEEIRNSAGLLQLDQELADLESKAAD 137
           PESQVGEE+D+DT+EWAMQDFYSLRKEVET SERVEEIRNSAGLLQLDQE+ADLESKAA+
Sbjct: 61  PESQVGEEVDSDTQEWAMQDFYSLRKEVETASERVEEIRNSAGLLQLDQEVADLESKAAE 120

Query: 138 DSFWDDRSKAQQVLVALTDVKDKIKMLTDFKTQVEEAETIVNLTEEMDSVDTGLLEEAAS 197
           +SFWDDRSKAQQVL+ALTDVKDKIKMLTDFKTQVEEAETIV LTEEMDSVDTGLLEEAAS
Sbjct: 121 NSFWDDRSKAQQVLMALTDVKDKIKMLTDFKTQVEEAETIVKLTEEMDSVDTGLLEEAAS 180

Query: 198 IIKDLNKALDQFELTQLLSGPYDKEGAVISISAGAGGTDAQDWADMLLRMYVRWGEKQRY 257
           IIKDLNKALDQFELTQLLSGPYDKEGAVI+ISAGAGGTDAQDWADMLLRMYVRWGEKQRY
Sbjct: 181 IIKDLNKALDQFELTQLLSGPYDKEGAVITISAGAGGTDAQDWADMLLRMYVRWGEKQRY 240

Query: 258 KTRVVEKSAGEEAGIKSATIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVE 317
           KTRVVEKSAGEEAGIKSATIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVE
Sbjct: 241 KTRVVEKSAGEEAGIKSATIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVE 300

Query: 318 VMPLLPEESMDVELPEEDLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQ 377
           VMPLLP+ESMDVE+PEEDLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQ
Sbjct: 301 VMPLLPDESMDVEIPEEDLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQ 360

Query: 378 LANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTG 437
           LANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTG
Sbjct: 361 LANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTG 420

Query: 438 CETSDIISVMDGELEPFIKAYLKYKYNMALSTGEVN 474
            ETSDI+SVMDGELEPFIKAYLKYKY+MAL+TGEVN
Sbjct: 421 HETSDIVSVMDGELEPFIKAYLKYKYSMALATGEVN 450

[Close]

BLAST of Lag0000775 vs. NCBI nr
Match: XP_023525876.1 (peptide chain release factor PrfB1, chloroplastic isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 807.4 bits (2084), Expect = 6.7e-230
Identity = 420/457 (91.90%), Postives = 438/457 (95.84%), Query Frame = 0


Query: 18  MEVVQRPLWLCSSPVFPLPKFQTTATSISLQFPLRPSSPSPYFRTSLSIHSRFS-HVPFA 77
           M+VVQRPLWLC+SPVFPLP+ QTT +S  LQFPL        FR+SLSIHSR S  VPFA
Sbjct: 1   MDVVQRPLWLCTSPVFPLPRLQTTRSSSELQFPL------SRFRSSLSIHSRSSLAVPFA 60

Query: 78  TPESQVGEELDADTREWAMQDFYSLRKEVETTSERVEEIRNSAGLLQLDQELADLESKAA 137
           TPES VGEE+D+DT+EWAMQDFYSLRKEVET SERVEEIRNSAGLLQLDQE+ADLESKAA
Sbjct: 61  TPESPVGEEVDSDTQEWAMQDFYSLRKEVETASERVEEIRNSAGLLQLDQEVADLESKAA 120

Query: 138 DDSFWDDRSKAQQVLVALTDVKDKIKMLTDFKTQVEEAETIVNLTEEMDSVDTGLLEEAA 197
           ++SFWDDRSKAQQVL+ALTDVKDKIKMLTDFKTQVEEAETIV LTEEMDSVDTGLLEEAA
Sbjct: 121 ENSFWDDRSKAQQVLMALTDVKDKIKMLTDFKTQVEEAETIVKLTEEMDSVDTGLLEEAA 180

Query: 198 SIIKDLNKALDQFELTQLLSGPYDKEGAVISISAGAGGTDAQDWADMLLRMYVRWGEKQR 257
           SIIKDLNKALDQFELTQLLSGPYDKEGAVI+ISAGAGGTDAQDWADMLLRMYVRWGEKQR
Sbjct: 181 SIIKDLNKALDQFELTQLLSGPYDKEGAVITISAGAGGTDAQDWADMLLRMYVRWGEKQR 240

Query: 258 YKTRVVEKSAGEEAGIKSATIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGV 317
           YKTRVVEKSAGEEAGIKSATIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGV
Sbjct: 241 YKTRVVEKSAGEEAGIKSATIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGV 300

Query: 318 EVMPLLPEESMDVELPEEDLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERS 377
           EVMPLLP+ESMDVE+PEEDLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERS
Sbjct: 301 EVMPLLPDESMDVEIPEEDLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERS 360

Query: 378 QLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRT 437
           QLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRT
Sbjct: 361 QLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRT 420

Query: 438 GCETSDIISVMDGELEPFIKAYLKYKYNMALSTGEVN 474
           G ETSDI+SVMDGELEPFIKAYLKYKY+MAL+TGEVN
Sbjct: 421 GHETSDIVSVMDGELEPFIKAYLKYKYSMALATGEVN 451

[Close]

BLAST of Lag0000775 vs. ExPASy Swiss-Prot
Match: Q9LVY0 (Peptide chain release factor PrfB1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PRFB1 PE=2 SV=1)

HSP 1 Score: 628.2 bits (1619), Expect = 7.3e-179
Identity = 325/431 (75.41%), Postives = 372/431 (86.31%), Query Frame = 0


Query: 51  LRPSSPSPYFRTSL-------SIHSRF-SHVPFATPESQVGEELDADTREWAMQDFYSLR 110
           LRP++  P  R SL       S  SRF S + FA  ES +    + +T EW MQDFY+LR
Sbjct: 27  LRPTN-LPLLRLSLPLSLPNFSSSSRFNSPIVFAAQESNLSVSNENETSEWLMQDFYTLR 86

Query: 111 KEVETTSERVEEIRNSAGLLQLDQELADLESKAADDSFWDDRSKAQQVLVALTDVKDKIK 170
           K+VE  S RVEEIR SA L QL+QE+ +LESKA D SFWDDR+KAQ+ L +L D+KD+++
Sbjct: 87  KDVEIASARVEEIRASANLQQLEQEITNLESKATDTSFWDDRTKAQETLSSLNDLKDRMR 146

Query: 171 MLTDFKTQVEEAETIVNLTEEMDSVDTGLLEEAASIIKDLNKALDQFELTQLLSGPYDKE 230
           +L++FKT VE+AETIV LTEEMDS D  LLEEA  IIK+LNK+LD+FELTQLLSGPYDKE
Sbjct: 147 LLSEFKTMVEDAETIVKLTEEMDSTDVSLLEEAMGIIKELNKSLDKFELTQLLSGPYDKE 206

Query: 231 GAVISISAGAGGTDAQDWADMLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKSATIEIEGR 290
           GAV+ I+AGAGGTDAQDWADMLLRMY+RWGEKQRYKT+VVE S GEEAGIKSAT+EIEGR
Sbjct: 207 GAVVYITAGAGGTDAQDWADMLLRMYMRWGEKQRYKTKVVEMSNGEEAGIKSATLEIEGR 266

Query: 291 YAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPEESMDVELPEEDLEISFSR 350
           YAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPEE++ +E+PEEDL+ISF+R
Sbjct: 267 YAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPEEAVGIEIPEEDLDISFTR 326

Query: 351 AGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQLANKIKALSRLKAKLLVIAEEQRAS 410
           AGGKGGQNVNKVETAVRITHIPTGV VRCTEERSQLANK +AL RLKAKL+VIAEEQRA+
Sbjct: 327 AGGKGGQNVNKVETAVRITHIPTGVAVRCTEERSQLANKTRALIRLKAKLMVIAEEQRAT 386

Query: 411 EIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTGCETSDIISVMDGELEPFIKAYLKYK 470
           EIK+IRGDAVKAEWGQQIRNYVFHPYKLVKDVRTG ETSDI SVMDG+L+PFIKAYLK+K
Sbjct: 387 EIKEIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTGHETSDITSVMDGDLDPFIKAYLKHK 446

Query: 471 YNMALSTGEVN 474
           Y +A+++   N
Sbjct: 447 YTLAMASAVTN 456

[Close]

BLAST of Lag0000775 vs. ExPASy Swiss-Prot
Match: P74476 (Peptide chain release factor 2 OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=prfB PE=3 SV=2)

HSP 1 Score: 422.2 bits (1084), Expect = 7.9e-117
Identity = 214/361 (59.28%), Postives = 276/361 (76.45%), Query Frame = 0


Query: 101 LRKEVETTSERVEEIRNSAGLLQLDQELADLESKAADDSFWDDRSKAQQVLVALTDVKDK 160
           L++ +E  S R+ + ++   L  L  ++ DLE  AA   FWDD  +AQQ+L  L + K +
Sbjct: 8   LKRNLELISSRLGQTQDYLDLPGLKAKVQDLEQCAAQPDFWDDTDQAQQILQTLNETKSQ 67

Query: 161 IKMLTDFKTQVEEAETIVNLTEEMDSVDTGLLEEAASIIKDLNKALDQFELTQLLSGPYD 220
           ++    ++ Q ++++ IV L E  D  D  LL EA + ++ L K LD++EL QLLSGPYD
Sbjct: 68  LEQWGIWQQQWQDSQAIVELLELED--DQALLTEAETTLEQLQKELDRWELQQLLSGPYD 127

Query: 221 KEGAVISISAGAGGTDAQDWADMLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKSATIEIE 280
            +GA ++I+AGAGGTDAQDWA+MLLRMY RW EKQ YK  + E S G+EAG+KS T+EIE
Sbjct: 128 AKGATLTINAGAGGTDAQDWAEMLLRMYTRWSEKQGYKVHLAEISEGDEAGLKSVTLEIE 187

Query: 281 GRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPEESMDVELPEEDLEISF 340
           GRYAYGY+  EKGTHR+VR SPFN+ G RQTSF+GVEVMPLL EE++ +++P++DL+IS 
Sbjct: 188 GRYAYGYLKSEKGTHRLVRISPFNANGKRQTSFAGVEVMPLLGEEAISLDIPDKDLDIST 247

Query: 341 SRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQLANKIKALSRLKAKLLVIAEEQR 400
           SRAGGKGGQNVNKVETAVRI H+PTG+ VRCT+ERSQL NK KAL+ LKAKLL++ EEQR
Sbjct: 248 SRAGGKGGQNVNKVETAVRIVHLPTGLAVRCTQERSQLQNKEKALAILKAKLLIVLEEQR 307

Query: 401 ASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTGCETSDIISVMDGELEPFIKAYLK 460
           A  I +IRGD V+A WG QIRNYVFHPY+LVKD+RT  ET+D+  VMDGEL  FI+AYL+
Sbjct: 308 AQAIAEIRGDMVEAAWGTQIRNYVFHPYQLVKDLRTNVETTDVGGVMDGELSDFIEAYLR 366

Query: 461 Y 462
           +
Sbjct: 368 H 366

[Close]

BLAST of Lag0000775 vs. ExPASy Swiss-Prot
Match: B7K0A6 (Peptide chain release factor 2 OS=Rippkaea orientalis (strain PCC 8801) OX=41431 GN=prfB PE=3 SV=1)

HSP 1 Score: 416.0 bits (1068), Expect = 5.7e-115
Identity = 210/360 (58.33%), Postives = 275/360 (76.39%), Query Frame = 0


Query: 101 LRKEVETTSERVEEIRNSAGLLQLDQELADLESKAADDSFWDDRSKAQQVLVALTDVKDK 160
           L++E++  + R+ + ++   L  L+  + DLE  AA   FWD++  AQ+ L  L D+K  
Sbjct: 6   LKREIQQIAARLGKTQDYLDLPALNANIQDLEQIAAQPDFWDNQETAQKTLQQLNDLKSS 65

Query: 161 IKMLTDFKTQVEEAETIVNLTEEMDSVDTGLLEEAASIIKDLNKALDQFELTQLLSGPYD 220
           ++    +  Q+E+ + I  L E  +  D  L EEA + +  LN  LD++EL +LLSG YD
Sbjct: 66  VEEYHQWMGQLEDLKAIAELLELEE--DATLNEEAEANLTQLNHELDRWELQRLLSGIYD 125

Query: 221 KEGAVISISAGAGGTDAQDWADMLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKSATIEIE 280
            +GAV++I+AGAGGTDAQDWA+MLLRMY RWGE+Q YK  + E S G+EAGIKSAT+EIE
Sbjct: 126 SKGAVLTINAGAGGTDAQDWAEMLLRMYTRWGEQQGYKVHLTEISEGDEAGIKSATLEIE 185

Query: 281 GRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPEESMDVELPEEDLEISF 340
           GRYAYGY+ GEKGTHR+VR SPFN+ G RQTSF+G+EVMP L EE + VE+PE+DLEI+ 
Sbjct: 186 GRYAYGYLKGEKGTHRLVRISPFNANGKRQTSFAGIEVMPALEEEDLKVEIPEKDLEITT 245

Query: 341 SRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQLANKIKALSRLKAKLLVIAEEQR 400
           +R+GGKGGQNVNKVETAVR+ H+PTG+ VRCT+ERSQL NK KAL+ LKAKLL+IA+EQR
Sbjct: 246 TRSGGKGGQNVNKVETAVRVVHLPTGIAVRCTQERSQLQNKEKALALLKAKLLIIAQEQR 305

Query: 401 ASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTGCETSDIISVMDGELEPFIKAYLK 460
           A  I +IRGD V+A WG QIRNYVFHPY++VKDVRT  ET+D+  VMDG+L+ FI++YL+
Sbjct: 306 AQAIAEIRGDMVEAAWGNQIRNYVFHPYQMVKDVRTNTETTDVNGVMDGKLDLFIESYLR 363

[Close]

BLAST of Lag0000775 vs. ExPASy Swiss-Prot
Match: Q7U3W6 (Peptide chain release factor 2 OS=Synechococcus sp. (strain WH8102) OX=84588 GN=prfB PE=3 SV=1)

HSP 1 Score: 392.9 bits (1008), Expect = 5.2e-108
Identity = 205/364 (56.32%), Postives = 266/364 (73.08%), Query Frame = 0


Query: 97  DFYSLRKEVETTSERVEEIRNSAGLLQLDQELADLESKAADDSFWDDRSKAQQVLVALTD 156
           D    ++++   ++R+   ++   +  L     DLE  AA   FWDD+  AQ+ +  L +
Sbjct: 2   DLTDFKRDLSELTDRLGHAQDCLDVPALKARQQDLEQLAAQPDFWDDQQAAQKQMRRLDE 61

Query: 157 VKDKIKMLTDFKTQVEEAETIVNLTEEMDSVDTGLLEEAASIIKDLNKALDQFELTQLLS 216
           VK +++ L D+   V++A+  + L E     D  +L EA   +  L + LD++EL +LLS
Sbjct: 62  VKAQLQQLADWGGAVDDAKATLELYEL--EPDEEMLTEAQEGLNQLRQGLDRWELERLLS 121

Query: 217 GPYDKEGAVISISAGAGGTDAQDWADMLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKSAT 276
           G YDKEGAV++I+AGAGGTDAQDWA MLLRMY RW E    K  V E S GEEAGIKS T
Sbjct: 122 GDYDKEGAVLTINAGAGGTDAQDWAQMLLRMYTRWAEDHGMKVTVDELSEGEEAGIKSCT 181

Query: 277 IEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPEESMDVELPEEDL 336
           IE+EGRYAYGY+  EKGTHR+VR SPFN+   RQTSF+G+EVMP + EE +D+++PE+DL
Sbjct: 182 IEVEGRYAYGYLRNEKGTHRLVRISPFNANDKRQTSFAGIEVMPKIDEE-VDIDIPEKDL 241

Query: 337 EISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQLANKIKALSRLKAKLLVIA 396
           E++ SR+GG GGQNVNKVETAVRI HIPTG+ VRCT+ERSQL NK KA++ LKAKLLVIA
Sbjct: 242 EVTTSRSGGAGGQNVNKVETAVRILHIPTGLAVRCTQERSQLQNKEKAMALLKAKLLVIA 301

Query: 397 EEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTGCETSDIISVMDGELEPFIK 456
           +EQRA+EI  IRGD V+A WG QIRNYVFHPY++VKD+RT  ET+D+ +VMDG L+PFI 
Sbjct: 302 QEQRAAEIADIRGDIVEAAWGNQIRNYVFHPYQMVKDLRTSEETNDVQAVMDGALDPFID 361

Query: 457 AYLK 461
           A L+
Sbjct: 362 ASLR 362

[Close]

BLAST of Lag0000775 vs. ExPASy Swiss-Prot
Match: O67695 (Peptide chain release factor 2 OS=Aquifex aeolicus (strain VF5) OX=224324 GN=prfB PE=3 SV=1)

HSP 1 Score: 324.3 bits (830), Expect = 2.3e-87
Identity = 165/362 (45.58%), Postives = 245/362 (67.68%), Query Frame = 0


Query: 101 LRKEVETTSERVEEIRNSAGLLQLDQELADLESKAADDSFWDDRSKAQQVLVALTDVKDK 160
           L+ +VE   +R+E+++      +L+ EL +L+ K ++ +FW+D+ KA+QV+     V++ 
Sbjct: 6   LKGKVEELRKRLEDVKKILSPEKLESELKELDQKMSEPNFWEDQEKAKQVIQRRKWVEET 65

Query: 161 IKMLTDFKTQVEEAETIVNLTEEMDSVDTGLLEEAASIIKDLNKALDQFELTQLLSGPYD 220
           +  L + +  V++ E +V +T E D+    +++E    IK++ + L + EL   LSG  D
Sbjct: 66  LNKLKNLEKSVKDLEELVEITSEEDTETWAMMDEE---IKEVERTLRELELKTYLSGEMD 125

Query: 221 KEGAVISISAGAGGTDAQDWADMLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKSATIEIE 280
            + A ++I AGAGGT+A DWADML RMY RW EK+ Y+  +++ +  + AGIKS T+ ++
Sbjct: 126 AKNAYLTIQAGAGGTEACDWADMLFRMYKRWAEKKGYEVELIDITPDDVAGIKSVTVLVK 185

Query: 281 GRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPEESMDVELPEEDLEISF 340
           G YAYGY+ GE+G HR+VR SPF++   R TSF+ V VMP + +E + +E+  EDL+I  
Sbjct: 186 GPYAYGYLKGEQGVHRLVRISPFDANARRHTSFAAVSVMPQI-DEDIKIEIKPEDLKIET 245

Query: 341 SRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQLANKIKALSRLKAKLLVIAEEQR 400
            RA G GGQ VNK +TAVRITHIPTG+TV C +ERSQ  NK KAL  LKAKL  +  ++ 
Sbjct: 246 FRASGAGGQYVNKTDTAVRITHIPTGITVSCQQERSQYQNKRKALELLKAKLYQLEMKKL 305

Query: 401 ASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTGCETSDIISVMDGELEPFIKAYLK 460
             + KQ  G+     WG QIR+YVFHPYKL+KD+RTG ET ++ +VMDGE++ FI++YLK
Sbjct: 306 EEKKKQYEGEKTDIGWGHQIRSYVFHPYKLIKDLRTGYETGNVEAVMDGEIDEFIESYLK 363

Query: 461 YK 463
           +K
Sbjct: 366 WK 363

[Close]

BLAST of Lag0000775 vs. ExPASy TrEMBL
Match: A0A6J1D3Q2 (peptide chain release factor PrfB1, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111016981 PE=3 SV=1)

HSP 1 Score: 821.6 bits (2121), Expect = 1.7e-234
Identity = 429/458 (93.67%), Postives = 439/458 (95.85%), Query Frame = 0


Query: 18  MEVVQRPLWLCS--SPVFPLPKFQTTATSISLQFPLRPSSPSPYFRTSLSIHSRFSHVPF 77
           M V+ RPLWLC   S VFP PKFQ+T TS+SL+FP  PSSPSP+F TSLSI SRFS V F
Sbjct: 1   MAVLLRPLWLCGSFSLVFPFPKFQSTRTSLSLEFPPPPSSPSPHFPTSLSIRSRFSIVSF 60

Query: 78  ATPESQVGEELDADTREWAMQDFYSLRKEVETTSERVEEIRNSAGLLQLDQELADLESKA 137
           ATPESQVGEELDADTREWAMQDFYSLRKEVETTSERVEEIRNSAGL QLDQELADLESKA
Sbjct: 61  ATPESQVGEELDADTREWAMQDFYSLRKEVETTSERVEEIRNSAGLFQLDQELADLESKA 120

Query: 138 ADDSFWDDRSKAQQVLVALTDVKDKIKMLTDFKTQVEEAETIVNLTEEMDSVDTGLLEEA 197
           AD++ WDDRSKAQQVLVALTDVKDKIKMLTDFKTQVEEAETIV LTEEMDSVDTGLLEEA
Sbjct: 121 ADNALWDDRSKAQQVLVALTDVKDKIKMLTDFKTQVEEAETIVKLTEEMDSVDTGLLEEA 180

Query: 198 ASIIKDLNKALDQFELTQLLSGPYDKEGAVISISAGAGGTDAQDWADMLLRMYVRWGEKQ 257
           ASIIKDLNKALDQFELTQLLSGPYDKEGAVISISAGAGGTDAQDWADMLLRMYVRWGEKQ
Sbjct: 181 ASIIKDLNKALDQFELTQLLSGPYDKEGAVISISAGAGGTDAQDWADMLLRMYVRWGEKQ 240

Query: 258 RYKTRVVEKSAGEEAGIKSATIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSG 317
           RYKTRVVEKSAGEEAGIKSATIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSG
Sbjct: 241 RYKTRVVEKSAGEEAGIKSATIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSG 300

Query: 318 VEVMPLLPEESMDVELPEEDLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEER 377
           VEVMPLLP+ESMDVELPEEDLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEER
Sbjct: 301 VEVMPLLPDESMDVELPEEDLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEER 360

Query: 378 SQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVR 437
           SQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVR
Sbjct: 361 SQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVR 420

Query: 438 TGCETSDIISVMDGELEPFIKAYLKYKYNMALSTGEVN 474
           TG ETSDI+SVMDGELEPFIKAYLKYKY+MALSTG VN
Sbjct: 421 TGYETSDIVSVMDGELEPFIKAYLKYKYSMALSTGGVN 458

[Close]

BLAST of Lag0000775 vs. ExPASy TrEMBL
Match: A0A6J1GD28 (peptide chain release factor PrfB1, chloroplastic isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111452850 PE=3 SV=1)

HSP 1 Score: 811.2 bits (2094), Expect = 2.2e-231
Identity = 421/456 (92.32%), Postives = 437/456 (95.83%), Query Frame = 0


Query: 18  MEVVQRPLWLCSSPVFPLPKFQTTATSISLQFPLRPSSPSPYFRTSLSIHSRFSHVPFAT 77
           M+VVQRPLWLC+SPVFPLP+ QT  TS  LQFPL        FRTSLSIHSR S V FAT
Sbjct: 1   MDVVQRPLWLCTSPVFPLPRLQTARTSSPLQFPL------SRFRTSLSIHSRSSLVRFAT 60

Query: 78  PESQVGEELDADTREWAMQDFYSLRKEVETTSERVEEIRNSAGLLQLDQELADLESKAAD 137
           PESQVGEE+D+DT+EWAMQDFYSLRKEVET SERVEEIRNSAGLLQLDQE+ADLESKAA+
Sbjct: 61  PESQVGEEVDSDTQEWAMQDFYSLRKEVETASERVEEIRNSAGLLQLDQEVADLESKAAE 120

Query: 138 DSFWDDRSKAQQVLVALTDVKDKIKMLTDFKTQVEEAETIVNLTEEMDSVDTGLLEEAAS 197
           +SFWDDRSKAQQVL+ALTDVKDKIKMLTDFKTQVEEAETIV LTEEMDSVDTGLLEEAAS
Sbjct: 121 NSFWDDRSKAQQVLMALTDVKDKIKMLTDFKTQVEEAETIVKLTEEMDSVDTGLLEEAAS 180

Query: 198 IIKDLNKALDQFELTQLLSGPYDKEGAVISISAGAGGTDAQDWADMLLRMYVRWGEKQRY 257
           IIKDLNKALDQFELTQLLSGPYDKEGAVI+ISAGAGGTDAQDWADMLLRMYVRWGEKQRY
Sbjct: 181 IIKDLNKALDQFELTQLLSGPYDKEGAVITISAGAGGTDAQDWADMLLRMYVRWGEKQRY 240

Query: 258 KTRVVEKSAGEEAGIKSATIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVE 317
           KTRVVEKSAGEEAGIKSATIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVE
Sbjct: 241 KTRVVEKSAGEEAGIKSATIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVE 300

Query: 318 VMPLLPEESMDVELPEEDLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQ 377
           VMPLLP+ESMDVE+PEEDLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQ
Sbjct: 301 VMPLLPDESMDVEIPEEDLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQ 360

Query: 378 LANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTG 437
           LANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTG
Sbjct: 361 LANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTG 420

Query: 438 CETSDIISVMDGELEPFIKAYLKYKYNMALSTGEVN 474
            ETSDI+SVMDGELEPFIKAYLKYKY+MAL+TGEVN
Sbjct: 421 HETSDIVSVMDGELEPFIKAYLKYKYSMALATGEVN 450

[Close]

BLAST of Lag0000775 vs. ExPASy TrEMBL
Match: A0A6J1KF53 (peptide chain release factor PrfB1, chloroplastic isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111493309 PE=3 SV=1)

HSP 1 Score: 809.3 bits (2089), Expect = 8.5e-231
Identity = 420/456 (92.11%), Postives = 437/456 (95.83%), Query Frame = 0


Query: 18  MEVVQRPLWLCSSPVFPLPKFQTTATSISLQFPLRPSSPSPYFRTSLSIHSRFSHVPFAT 77
           M+VVQRPLWLC+SPVFP P+ QTT TS  LQ+PL        FRTSLSIHSR S V FAT
Sbjct: 1   MDVVQRPLWLCTSPVFPPPRLQTTRTSSPLQYPL------SRFRTSLSIHSRSSLVRFAT 60

Query: 78  PESQVGEELDADTREWAMQDFYSLRKEVETTSERVEEIRNSAGLLQLDQELADLESKAAD 137
           PESQVGEE+D+DT+EWAMQDFYSLRKEVET SERVEEIRNSAGLLQLDQE+ADLESKAA+
Sbjct: 61  PESQVGEEVDSDTQEWAMQDFYSLRKEVETASERVEEIRNSAGLLQLDQEVADLESKAAE 120

Query: 138 DSFWDDRSKAQQVLVALTDVKDKIKMLTDFKTQVEEAETIVNLTEEMDSVDTGLLEEAAS 197
           +SFWDDRSKAQQVL+ALTDVKDKIKMLTDFKTQVEEAETIV LTEEMDSVDTGLLEEAAS
Sbjct: 121 NSFWDDRSKAQQVLMALTDVKDKIKMLTDFKTQVEEAETIVKLTEEMDSVDTGLLEEAAS 180

Query: 198 IIKDLNKALDQFELTQLLSGPYDKEGAVISISAGAGGTDAQDWADMLLRMYVRWGEKQRY 257
           IIKDLNKALDQFELTQLLSGPYDKEGAVI+ISAGAGGTDAQDWADMLLRMYVRWGEKQRY
Sbjct: 181 IIKDLNKALDQFELTQLLSGPYDKEGAVITISAGAGGTDAQDWADMLLRMYVRWGEKQRY 240

Query: 258 KTRVVEKSAGEEAGIKSATIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVE 317
           KTRVVEKSAGEEAGIKSATIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVE
Sbjct: 241 KTRVVEKSAGEEAGIKSATIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVE 300

Query: 318 VMPLLPEESMDVELPEEDLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQ 377
           VMPLLP+ESMDVE+PEEDLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQ
Sbjct: 301 VMPLLPDESMDVEIPEEDLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQ 360

Query: 378 LANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTG 437
           LANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTG
Sbjct: 361 LANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTG 420

Query: 438 CETSDIISVMDGELEPFIKAYLKYKYNMALSTGEVN 474
            ETSDI+SVMDGELEPFIKAYLKYKY+MAL+TGEVN
Sbjct: 421 HETSDIVSVMDGELEPFIKAYLKYKYSMALATGEVN 450

[Close]

BLAST of Lag0000775 vs. ExPASy TrEMBL
Match: A0A6J1GCA2 (peptide chain release factor PrfB1, chloroplastic isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111452850 PE=3 SV=1)

HSP 1 Score: 807.0 bits (2083), Expect = 4.2e-230
Identity = 421/457 (92.12%), Postives = 437/457 (95.62%), Query Frame = 0


Query: 18  MEVVQRPLWLCSSPVFPLPKFQTTATSISLQFPLRPSSPSPYFRTSLSIHSRFS-HVPFA 77
           M+VVQRPLWLC+SPVFPLP+ QT  TS  LQFPL        FRTSLSIHSR S  V FA
Sbjct: 1   MDVVQRPLWLCTSPVFPLPRLQTARTSSPLQFPL------SRFRTSLSIHSRSSLAVRFA 60

Query: 78  TPESQVGEELDADTREWAMQDFYSLRKEVETTSERVEEIRNSAGLLQLDQELADLESKAA 137
           TPESQVGEE+D+DT+EWAMQDFYSLRKEVET SERVEEIRNSAGLLQLDQE+ADLESKAA
Sbjct: 61  TPESQVGEEVDSDTQEWAMQDFYSLRKEVETASERVEEIRNSAGLLQLDQEVADLESKAA 120

Query: 138 DDSFWDDRSKAQQVLVALTDVKDKIKMLTDFKTQVEEAETIVNLTEEMDSVDTGLLEEAA 197
           ++SFWDDRSKAQQVL+ALTDVKDKIKMLTDFKTQVEEAETIV LTEEMDSVDTGLLEEAA
Sbjct: 121 ENSFWDDRSKAQQVLMALTDVKDKIKMLTDFKTQVEEAETIVKLTEEMDSVDTGLLEEAA 180

Query: 198 SIIKDLNKALDQFELTQLLSGPYDKEGAVISISAGAGGTDAQDWADMLLRMYVRWGEKQR 257
           SIIKDLNKALDQFELTQLLSGPYDKEGAVI+ISAGAGGTDAQDWADMLLRMYVRWGEKQR
Sbjct: 181 SIIKDLNKALDQFELTQLLSGPYDKEGAVITISAGAGGTDAQDWADMLLRMYVRWGEKQR 240

Query: 258 YKTRVVEKSAGEEAGIKSATIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGV 317
           YKTRVVEKSAGEEAGIKSATIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGV
Sbjct: 241 YKTRVVEKSAGEEAGIKSATIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGV 300

Query: 318 EVMPLLPEESMDVELPEEDLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERS 377
           EVMPLLP+ESMDVE+PEEDLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERS
Sbjct: 301 EVMPLLPDESMDVEIPEEDLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERS 360

Query: 378 QLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRT 437
           QLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRT
Sbjct: 361 QLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRT 420

Query: 438 GCETSDIISVMDGELEPFIKAYLKYKYNMALSTGEVN 474
           G ETSDI+SVMDGELEPFIKAYLKYKY+MAL+TGEVN
Sbjct: 421 GHETSDIVSVMDGELEPFIKAYLKYKYSMALATGEVN 451

[Close]

BLAST of Lag0000775 vs. ExPASy TrEMBL
Match: A0A6J1KHL0 (peptide chain release factor PrfB1, chloroplastic isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111493309 PE=3 SV=1)

HSP 1 Score: 805.1 bits (2078), Expect = 1.6e-229
Identity = 420/457 (91.90%), Postives = 437/457 (95.62%), Query Frame = 0


Query: 18  MEVVQRPLWLCSSPVFPLPKFQTTATSISLQFPLRPSSPSPYFRTSLSIHSRFS-HVPFA 77
           M+VVQRPLWLC+SPVFP P+ QTT TS  LQ+PL        FRTSLSIHSR S  V FA
Sbjct: 1   MDVVQRPLWLCTSPVFPPPRLQTTRTSSPLQYPL------SRFRTSLSIHSRSSLAVRFA 60

Query: 78  TPESQVGEELDADTREWAMQDFYSLRKEVETTSERVEEIRNSAGLLQLDQELADLESKAA 137
           TPESQVGEE+D+DT+EWAMQDFYSLRKEVET SERVEEIRNSAGLLQLDQE+ADLESKAA
Sbjct: 61  TPESQVGEEVDSDTQEWAMQDFYSLRKEVETASERVEEIRNSAGLLQLDQEVADLESKAA 120

Query: 138 DDSFWDDRSKAQQVLVALTDVKDKIKMLTDFKTQVEEAETIVNLTEEMDSVDTGLLEEAA 197
           ++SFWDDRSKAQQVL+ALTDVKDKIKMLTDFKTQVEEAETIV LTEEMDSVDTGLLEEAA
Sbjct: 121 ENSFWDDRSKAQQVLMALTDVKDKIKMLTDFKTQVEEAETIVKLTEEMDSVDTGLLEEAA 180

Query: 198 SIIKDLNKALDQFELTQLLSGPYDKEGAVISISAGAGGTDAQDWADMLLRMYVRWGEKQR 257
           SIIKDLNKALDQFELTQLLSGPYDKEGAVI+ISAGAGGTDAQDWADMLLRMYVRWGEKQR
Sbjct: 181 SIIKDLNKALDQFELTQLLSGPYDKEGAVITISAGAGGTDAQDWADMLLRMYVRWGEKQR 240

Query: 258 YKTRVVEKSAGEEAGIKSATIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGV 317
           YKTRVVEKSAGEEAGIKSATIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGV
Sbjct: 241 YKTRVVEKSAGEEAGIKSATIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGV 300

Query: 318 EVMPLLPEESMDVELPEEDLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERS 377
           EVMPLLP+ESMDVE+PEEDLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERS
Sbjct: 301 EVMPLLPDESMDVEIPEEDLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERS 360

Query: 378 QLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRT 437
           QLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRT
Sbjct: 361 QLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRT 420

Query: 438 GCETSDIISVMDGELEPFIKAYLKYKYNMALSTGEVN 474
           G ETSDI+SVMDGELEPFIKAYLKYKY+MAL+TGEVN
Sbjct: 421 GHETSDIVSVMDGELEPFIKAYLKYKYSMALATGEVN 451

[Close]

BLAST of Lag0000775 vs. TAIR 10
Match: AT5G36170.2 (high chlorophyll fluorescent 109 )

HSP 1 Score: 629.8 bits (1623), Expect = 1.8e-180
Identity = 324/430 (75.35%), Postives = 371/430 (86.28%), Query Frame = 0


Query: 51  LRPSSPSPYFRTSL-------SIHSRFSHVPFATPESQVGEELDADTREWAMQDFYSLRK 110
           LRP++  P  R SL       S  SRF+   FA  ES +    + +T EW MQDFY+LRK
Sbjct: 27  LRPTN-LPLLRLSLPLSLPNFSSSSRFNSPIFAAQESNLSVSNENETSEWLMQDFYTLRK 86

Query: 111 EVETTSERVEEIRNSAGLLQLDQELADLESKAADDSFWDDRSKAQQVLVALTDVKDKIKM 170
           +VE  S RVEEIR SA L QL+QE+ +LESKA D SFWDDR+KAQ+ L +L D+KD++++
Sbjct: 87  DVEIASARVEEIRASANLQQLEQEITNLESKATDTSFWDDRTKAQETLSSLNDLKDRMRL 146

Query: 171 LTDFKTQVEEAETIVNLTEEMDSVDTGLLEEAASIIKDLNKALDQFELTQLLSGPYDKEG 230
           L++FKT VE+AETIV LTEEMDS D  LLEEA  IIK+LNK+LD+FELTQLLSGPYDKEG
Sbjct: 147 LSEFKTMVEDAETIVKLTEEMDSTDVSLLEEAMGIIKELNKSLDKFELTQLLSGPYDKEG 206

Query: 231 AVISISAGAGGTDAQDWADMLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKSATIEIEGRY 290
           AV+ I+AGAGGTDAQDWADMLLRMY+RWGEKQRYKT+VVE S GEEAGIKSAT+EIEGRY
Sbjct: 207 AVVYITAGAGGTDAQDWADMLLRMYMRWGEKQRYKTKVVEMSNGEEAGIKSATLEIEGRY 266

Query: 291 AYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPEESMDVELPEEDLEISFSRA 350
           AYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPEE++ +E+PEEDL+ISF+RA
Sbjct: 267 AYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPEEAVGIEIPEEDLDISFTRA 326

Query: 351 GGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQLANKIKALSRLKAKLLVIAEEQRASE 410
           GGKGGQNVNKVETAVRITHIPTGV VRCTEERSQLANK +AL RLKAKL+VIAEEQRA+E
Sbjct: 327 GGKGGQNVNKVETAVRITHIPTGVAVRCTEERSQLANKTRALIRLKAKLMVIAEEQRATE 386

Query: 411 IKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTGCETSDIISVMDGELEPFIKAYLKYKY 470
           IK+IRGDAVKAEWGQQIRNYVFHPYKLVKDVRTG ETSDI SVMDG+L+PFIKAYLK+KY
Sbjct: 387 IKEIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTGHETSDITSVMDGDLDPFIKAYLKHKY 446

Query: 471 NMALSTGEVN 474
            +A+++   N
Sbjct: 447 TLAMASAVTN 455

[Close]

BLAST of Lag0000775 vs. TAIR 10
Match: AT5G36170.1 (high chlorophyll fluorescent 109 )

HSP 1 Score: 628.2 bits (1619), Expect = 5.2e-180
Identity = 325/431 (75.41%), Postives = 372/431 (86.31%), Query Frame = 0


Query: 51  LRPSSPSPYFRTSL-------SIHSRF-SHVPFATPESQVGEELDADTREWAMQDFYSLR 110
           LRP++  P  R SL       S  SRF S + FA  ES +    + +T EW MQDFY+LR
Sbjct: 27  LRPTN-LPLLRLSLPLSLPNFSSSSRFNSPIVFAAQESNLSVSNENETSEWLMQDFYTLR 86

Query: 111 KEVETTSERVEEIRNSAGLLQLDQELADLESKAADDSFWDDRSKAQQVLVALTDVKDKIK 170
           K+VE  S RVEEIR SA L QL+QE+ +LESKA D SFWDDR+KAQ+ L +L D+KD+++
Sbjct: 87  KDVEIASARVEEIRASANLQQLEQEITNLESKATDTSFWDDRTKAQETLSSLNDLKDRMR 146

Query: 171 MLTDFKTQVEEAETIVNLTEEMDSVDTGLLEEAASIIKDLNKALDQFELTQLLSGPYDKE 230
           +L++FKT VE+AETIV LTEEMDS D  LLEEA  IIK+LNK+LD+FELTQLLSGPYDKE
Sbjct: 147 LLSEFKTMVEDAETIVKLTEEMDSTDVSLLEEAMGIIKELNKSLDKFELTQLLSGPYDKE 206

Query: 231 GAVISISAGAGGTDAQDWADMLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKSATIEIEGR 290
           GAV+ I+AGAGGTDAQDWADMLLRMY+RWGEKQRYKT+VVE S GEEAGIKSAT+EIEGR
Sbjct: 207 GAVVYITAGAGGTDAQDWADMLLRMYMRWGEKQRYKTKVVEMSNGEEAGIKSATLEIEGR 266

Query: 291 YAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPEESMDVELPEEDLEISFSR 350
           YAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPEE++ +E+PEEDL+ISF+R
Sbjct: 267 YAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPEEAVGIEIPEEDLDISFTR 326

Query: 351 AGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQLANKIKALSRLKAKLLVIAEEQRAS 410
           AGGKGGQNVNKVETAVRITHIPTGV VRCTEERSQLANK +AL RLKAKL+VIAEEQRA+
Sbjct: 327 AGGKGGQNVNKVETAVRITHIPTGVAVRCTEERSQLANKTRALIRLKAKLMVIAEEQRAT 386

Query: 411 EIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTGCETSDIISVMDGELEPFIKAYLKYK 470
           EIK+IRGDAVKAEWGQQIRNYVFHPYKLVKDVRTG ETSDI SVMDG+L+PFIKAYLK+K
Sbjct: 387 EIKEIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTGHETSDITSVMDGDLDPFIKAYLKHK 446

Query: 471 YNMALSTGEVN 474
           Y +A+++   N
Sbjct: 447 YTLAMASAVTN 456

[Close]

BLAST of Lag0000775 vs. TAIR 10
Match: AT5G36170.3 (high chlorophyll fluorescent 109 )

HSP 1 Score: 464.9 bits (1195), Expect = 7.6e-131
Identity = 243/331 (73.41%), Postives = 280/331 (84.59%), Query Frame = 0


Query: 51  LRPSSPSPYFRTSL-------SIHSRF-SHVPFATPESQVGEELDADTREWAMQDFYSLR 110
           LRP++  P  R SL       S  SRF S + FA  ES +    + +T EW MQDFY+LR
Sbjct: 27  LRPTN-LPLLRLSLPLSLPNFSSSSRFNSPIVFAAQESNLSVSNENETSEWLMQDFYTLR 86

Query: 111 KEVETTSERVEEIRNSAGLLQLDQELADLESKAADDSFWDDRSKAQQVLVALTDVKDKIK 170
           K+VE  S RVEEIR SA L QL+QE+ +LESKA D SFWDDR+KAQ+ L +L D+KD+++
Sbjct: 87  KDVEIASARVEEIRASANLQQLEQEITNLESKATDTSFWDDRTKAQETLSSLNDLKDRMR 146

Query: 171 MLTDFKTQVEEAETIVNLTEEMDSVDTGLLEEAASIIKDLNKALDQFELTQLLSGPYDKE 230
           +L++FKT VE+AETIV LTEEMDS D  LLEEA  IIK+LNK+LD+FELTQLLSGPYDKE
Sbjct: 147 LLSEFKTMVEDAETIVKLTEEMDSTDVSLLEEAMGIIKELNKSLDKFELTQLLSGPYDKE 206

Query: 231 GAVISISAGAGGTDAQDWADMLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKSATIEIEGR 290
           GAV+ I+AGAGGTDAQDWADMLLRMY+RWGEKQRYKT+VVE S GEEAGIKSAT+EIEGR
Sbjct: 207 GAVVYITAGAGGTDAQDWADMLLRMYMRWGEKQRYKTKVVEMSNGEEAGIKSATLEIEGR 266

Query: 291 YAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPEESMDVELPEEDLEISFSR 350
           YAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPEE++ +E+PEEDL+ISF+R
Sbjct: 267 YAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPEEAVGIEIPEEDLDISFTR 326

Query: 351 AGGKGGQNVNKVETAVRITHIPTGVTVRCTE 374
           AGGKGGQNVNKVETAVRITHIPTGV VRCT+
Sbjct: 327 AGGKGGQNVNKVETAVRITHIPTGVAVRCTD 356

[Close]

BLAST of Lag0000775 vs. TAIR 10
Match: AT1G56350.1 (Peptide chain release factor 2 )

HSP 1 Score: 238.4 bits (607), Expect = 1.2e-62
Identity = 135/374 (36.10%), Postives = 212/374 (56.68%), Query Frame = 0


Query: 86  LDADTREWAMQDFYSLRKEVETTSERVEEIRNSAGLLQLDQELADLESKAADDSFWDDRS 145
           LD +  +W        +      ++ ++ I+      +L   L  L ++      WDD +
Sbjct: 108 LDENEGDW--------KSHAAAIAQSIQVIKRRLQWKKLLVRLKVLSAELNKSDLWDDPT 167

Query: 146 KAQQVLVALTDVKDKIKMLTDFKTQVEEAETIVNLTEEMDSVDTGLLEEAASIIKDLNKA 205
            A ++      +  K+K +  F+ ++ E   ++ L +E +  D+ L  E    + D+ + 
Sbjct: 168 HAGKISREHGSLTGKMKGVMTFERELLEHIDMLKLAKEEN--DSELESETLKALIDMRRV 227

Query: 206 LDQFELTQLLSGPYDKEGAVISISAGAGGTDAQDWADMLLRMYVRWGEKQRYKTRVVEKS 265
             + EL  LLS   D     I + AGAGGT++ DWA M++ MY  W +++++   VV+++
Sbjct: 228 SKEKELEALLSADNDPCSCYIEVQAGAGGTESNDWAAMVMEMYKTWAQRRKFSVTVVDEA 287

Query: 266 AGEEAGIKSATIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPEE 325
            GE AGIK ATI++ G YAYGY   E G HR+VR SPF+S   R TSF+ V V+P+L + 
Sbjct: 288 PGEIAGIKRATIKVNGEYAYGYAKAEVGVHRLVRISPFDSGKRRHTSFAAVAVIPILGDG 347

Query: 326 SMDVELPEEDLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQLANKIKAL 385
           S  VE+ + DL I   R+GG GGQ+ N  ++AVRI HIPTG+T  C  ERSQ +NK  A+
Sbjct: 348 STRVEINDSDLRIERFRSGGAGGQHANTTDSAVRIVHIPTGITATCQNERSQHSNKASAM 407

Query: 386 SRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTGCETSDIIS 445
           + L+++L  +   ++ +   Q      +  WG QIR YV HPY++VKD+RT  E SD  S
Sbjct: 408 AVLQSRLDQLEMARQTAMNAQHTQSLTEISWGNQIRTYVLHPYRMVKDLRTNYEVSDPDS 467

Query: 446 VMDGELEPFIKAYL 460
           V++G+L+ FI ++L
Sbjct: 468 VLEGDLDGFILSFL 471

[Close]

BLAST of Lag0000775 vs. TAIR 10
Match: AT3G57190.1 (peptide chain release factor, putative )

HSP 1 Score: 215.7 bits (548), Expect = 8.0e-56
Identity = 130/366 (35.52%), Postives = 216/366 (59.02%), Query Frame = 0


Query: 99  YSLRKEVETTSERVEEIRNSAGLLQLDQELADLESKAADDSFWDDRSKAQQVLVALTDVK 158
           +SL+K+++    + E     A  L+ +Q +   E+    D  WDD +K+ ++L+ L D  
Sbjct: 48  FSLKKKIKDVVLKAEMFAPDALELEEEQWIKQEETMRYFD-LWDDPAKSDEILLKLADRA 107

Query: 159 DKIKMLTDFKTQVEEAETIVNLTEEMDSVDTGLLEEAASIIKDLNKALDQFELTQLLSGP 218
             +  L D K + EEA+ I+ L  EMD++D  L E+A     D++++L  +E+++LL   
Sbjct: 108 KAVDSLKDLKYKAEEAKLIIQL-GEMDAIDYSLFEQAYDSSLDVSRSLHHYEMSKLLRDQ 167

Query: 219 YDKEGAVISISAGAGGTDAQDWADMLLRMYVRWGEKQRYKTRVVEKSA--GEEAGIKSAT 278
           YD EGA + I +G+ G  +Q W + ++ MY++W E+     RV EK +    ++G+ SAT
Sbjct: 168 YDAEGACMIIKSGSPGAKSQIWTEQVVSMYIKWAERLGQNARVAEKCSLLSNKSGVSSAT 227

Query: 279 IEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPEESMDVELPEEDL 338
           IE E  +AYGY+ GE+G HR++  S  N     +   + V+++PL    S D E+ E DL
Sbjct: 228 IEFEFEFAYGYLLGERGVHRLIISSTSN-----EECSATVDIIPLFLRASPDFEVKEGDL 287

Query: 339 EISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQLANKIKALSRLKAKLLVIA 398
            +S+        ++    E  V I HIP+GVT++ + ER++ AN+IKAL+RLKAKLLVIA
Sbjct: 288 IVSY-----PAKEDHKIAENMVCIHHIPSGVTLQSSGERNRFANRIKALNRLKAKLLVIA 347

Query: 399 EEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTGCETSDIISVMDGELEPFIK 458
           +EQ+ S++ +I    +  E  ++ R+YV   +K+V D +TG E  D+ SV+DG + P + 
Sbjct: 348 KEQKVSDVNKIDSKNI-LEPREETRSYVSKGHKMVVDRKTGLEILDLKSVLDGNIGPLLG 400

Query: 459 AYLKYK 463
           A++  +
Sbjct: 408 AHISMR 400

The following BLAST results are available for this feature:

BLAST of Lag0000775 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022148293.1	3.4e-234	93.67	peptide chain release factor PrfB1, chloroplastic [Momordica charantia]	[more]
XP_023525877.1	3.5e-231	92.11	peptide chain release factor PrfB1, chloroplastic isoform X2 [Cucurbita pepo sub...	[more]
XP_022949524.1	4.6e-231	92.32	peptide chain release factor PrfB1, chloroplastic isoform X2 [Cucurbita moschata...	[more]
XP_022998734.1	1.8e-230	92.11	peptide chain release factor PrfB1, chloroplastic isoform X2 [Cucurbita maxima]	[more]
XP_023525876.1	6.7e-230	91.90	peptide chain release factor PrfB1, chloroplastic isoform X1 [Cucurbita pepo sub...	[more]

BLAST of Lag0000775 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LVY0	7.3e-179	75.41	Peptide chain release factor PrfB1, chloroplastic OS=Arabidopsis thaliana OX=370...	[more]
P74476	7.9e-117	59.28	Peptide chain release factor 2 OS=Synechocystis sp. (strain PCC 6803 / Kazusa) O...	[more]
B7K0A6	5.7e-115	58.33	Peptide chain release factor 2 OS=Rippkaea orientalis (strain PCC 8801) OX=41431...	[more]
Q7U3W6	5.2e-108	56.32	Peptide chain release factor 2 OS=Synechococcus sp. (strain WH8102) OX=84588 GN=...	[more]
O67695	2.3e-87	45.58	Peptide chain release factor 2 OS=Aquifex aeolicus (strain VF5) OX=224324 GN=prf...	[more]

BLAST of Lag0000775 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D3Q2	1.7e-234	93.67	peptide chain release factor PrfB1, chloroplastic OS=Momordica charantia OX=3673...	[more]
A0A6J1GD28	2.2e-231	92.32	peptide chain release factor PrfB1, chloroplastic isoform X2 OS=Cucurbita moscha...	[more]
A0A6J1KF53	8.5e-231	92.11	peptide chain release factor PrfB1, chloroplastic isoform X2 OS=Cucurbita maxima...	[more]
A0A6J1GCA2	4.2e-230	92.12	peptide chain release factor PrfB1, chloroplastic isoform X1 OS=Cucurbita moscha...	[more]
A0A6J1KHL0	1.6e-229	91.90	peptide chain release factor PrfB1, chloroplastic isoform X1 OS=Cucurbita maxima...	[more]

BLAST of Lag0000775 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G36170.2	1.8e-180	75.35	high chlorophyll fluorescent 109	[more]
AT5G36170.1	5.2e-180	75.41	high chlorophyll fluorescent 109	[more]
AT5G36170.3	7.6e-131	73.41	high chlorophyll fluorescent 109	[more]
AT1G56350.1	1.2e-62	36.10	Peptide chain release factor 2	[more]
AT3G57190.1	8.0e-56	35.52	peptide chain release factor, putative	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 192..212
None	No IPR available	GENE3D	3.30.70.1660		coord: 226..456 e-value: 1.7E-87 score: 294.1
None	No IPR available	GENE3D	1.20.58.410	Release factor	coord: 103..219 e-value: 1.3E-12 score: 49.8
None	No IPR available	GENE3D	3.30.160.20		coord: 322..418 e-value: 1.7E-87 score: 294.1
None	No IPR available	PANTHER	PTHR43116:SF3	PEPTIDE CHAIN RELEASE FACTOR PRFB1, CHLOROPLASTIC-RELATED	coord: 78..466
None	No IPR available	PANTHER	PTHR43116	PEPTIDE CHAIN RELEASE FACTOR 2	coord: 78..466
None	No IPR available	SUPERFAMILY	75620	Release factor	coord: 124..464
IPR005139	Peptide chain release factor	SMART	SM00937	PCRF_a_2	coord: 179..291 e-value: 2.6E-42 score: 156.6
IPR005139	Peptide chain release factor	PFAM	PF03462	PCRF	coord: 125..316 e-value: 5.5E-52 score: 176.4
IPR004374	Peptide chain release factor 2	TIGRFAM	TIGR00020	TIGR00020	coord: 99..462 e-value: 4.8E-124 score: 411.9
IPR004374	Peptide chain release factor 2	HAMAP	MF_00094	Rel_fac_2	coord: 97..463 score: 36.657173
IPR000352	Peptide chain release factor class I	PFAM	PF00472	RF-1	coord: 327..435 e-value: 4.3E-35 score: 120.1
IPR000352	Peptide chain release factor class I	PROSITE	PS00745	RF_PROK_I	coord: 342..358

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0000775.1	Lag0000775.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006415	translational termination
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0016149	translation release factor activity, codon specific
molecular_function	GO:0003747	translation release factor activity