Lag0000609 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0000609 vs. NCBI nr
Match: XP_022948283.1 (probable carboxylesterase 17 [Cucurbita moschata])

HSP 1 Score: 654.1 bits (1686), Expect = 6.9e-184
Identity = 309/343 (90.09%), Postives = 329/343 (95.92%), Query Frame = 0

Query: 1   MAAMYIDPRLKLQMPKPHLQNGATAEEIEGLIRVYADGHIERPPIIPNVSCSTTASDKGV 60
           MAAMYIDPRLKLQM KPHLQNG T+EEIEGLIRVYADGH+ERPPIIPNV+CSTTASDKGV
Sbjct: 6   MAAMYIDPRLKLQMAKPHLQNGGTSEEIEGLIRVYADGHVERPPIIPNVACSTTASDKGV 65

Query: 61  TAKDVLIDNFTNTWARIYIPNSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLIDLTSKSKC 120
           TAKDVLID FTNTWARIYIPNSQRNLPLLVYFHGGGFCVGSAAWSCYHEFL++L SK+KC
Sbjct: 66  TAKDVLIDIFTNTWARIYIPNSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKAKC 125

Query: 121 VVLSVNYRLSPENRLPAAYEDGFNALMWLKQQAVTDPIEHKWWLGNCNPSKLFLAGDSAG 180
           V+LS+NYRLSPENRLPAAY+DGFNALMWLKQQA+T+PIEHKWWLGNCNPSKLFLAGDSAG
Sbjct: 126 VILSINYRLSPENRLPAAYDDGFNALMWLKQQAITNPIEHKWWLGNCNPSKLFLAGDSAG 185

Query: 181 ANIAYNIATRLGLNESTILKPLTLKGIILIQPFFGGESRTRSEKLAAQPSSASALTLAAA 240
           ANI+YN ATR+G NEST+LKPLTLKGIILIQPFFGGESRT+SEKLAA PSS SALTL+AA
Sbjct: 186 ANISYNTATRIGHNESTLLKPLTLKGIILIQPFFGGESRTKSEKLAATPSSTSALTLSAA 245

Query: 241 DTYWRLSLPPAVNRDHPWSNPLVNGGTKLKETKLPPLIVCISELDILMDRNLEFCAAMAG 300
           DTYWRLSLPP  NRDHPWSNPLVNGG KLKE KLPPL+VCISELDIL+DRNLEFCAAMAG
Sbjct: 246 DTYWRLSLPPTANRDHPWSNPLVNGGAKLKEMKLPPLMVCISELDILLDRNLEFCAAMAG 305

Query: 301 AGKKVERVILKGVGHGFQILNKDQISTLRTQEMMGSIQAFIQQ 344
           AGKKVERVILKGVGHGFQILNKDQIST RTQE+MGSI+AFI Q
Sbjct: 306 AGKKVERVILKGVGHGFQILNKDQISTQRTQELMGSIRAFIHQ 348

BLAST of Lag0000609 vs. NCBI nr
Match: XP_023523365.1 (probable carboxylesterase 17 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 651.7 bits (1680), Expect = 3.4e-183
Identity = 307/343 (89.50%), Postives = 328/343 (95.63%), Query Frame = 0

Query: 1   MAAMYIDPRLKLQMPKPHLQNGATAEEIEGLIRVYADGHIERPPIIPNVSCSTTASDKGV 60
           MAAMY+DPRLKLQM KPHLQNG T+EEIEGLIRVYADGH+ERPPIIPNV+CSTTASDKGV
Sbjct: 6   MAAMYVDPRLKLQMAKPHLQNGGTSEEIEGLIRVYADGHVERPPIIPNVACSTTASDKGV 65

Query: 61  TAKDVLIDNFTNTWARIYIPNSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLIDLTSKSKC 120
           TAKDVLID FTNTWARIYIPNSQRNLPLLVYFHGGGFCVGSAAWSCYHEFL++L SK+KC
Sbjct: 66  TAKDVLIDIFTNTWARIYIPNSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKAKC 125

Query: 121 VVLSVNYRLSPENRLPAAYEDGFNALMWLKQQAVTDPIEHKWWLGNCNPSKLFLAGDSAG 180
           V+LS+NYRLSPENRLPAAY+DGFNALMWLKQQA+T+PIEHKWW GNCNPSKLFLAGDSAG
Sbjct: 126 VILSINYRLSPENRLPAAYDDGFNALMWLKQQAITNPIEHKWWSGNCNPSKLFLAGDSAG 185

Query: 181 ANIAYNIATRLGLNESTILKPLTLKGIILIQPFFGGESRTRSEKLAAQPSSASALTLAAA 240
           ANI YN ATRLG NEST+LKPLTLKGIILIQPFFGGESRT+SEKLAA PSS SALTL+A+
Sbjct: 186 ANIVYNTATRLGHNESTLLKPLTLKGIILIQPFFGGESRTKSEKLAATPSSTSALTLSAS 245

Query: 241 DTYWRLSLPPAVNRDHPWSNPLVNGGTKLKETKLPPLIVCISELDILMDRNLEFCAAMAG 300
           DTYWRLSLPP  NRDHPWSNPLVNGG KLKETKLPPL+VCISELDIL+DRNLEFCAAMAG
Sbjct: 246 DTYWRLSLPPTANRDHPWSNPLVNGGAKLKETKLPPLMVCISELDILLDRNLEFCAAMAG 305

Query: 301 AGKKVERVILKGVGHGFQILNKDQISTLRTQEMMGSIQAFIQQ 344
           AGKKV+RVILKGVGHGFQILNKDQIST RTQE+MGSI+AFI Q
Sbjct: 306 AGKKVDRVILKGVGHGFQILNKDQISTQRTQELMGSIRAFIHQ 348

BLAST of Lag0000609 vs. NCBI nr
Match: XP_022998605.1 (probable carboxylesterase 17 [Cucurbita maxima])

HSP 1 Score: 649.4 bits (1674), Expect = 1.7e-182
Identity = 308/343 (89.80%), Postives = 328/343 (95.63%), Query Frame = 0

Query: 1   MAAMYIDPRLKLQMPKPHLQNGATAEEIEGLIRVYADGHIERPPIIPNVSCSTTASDKGV 60
           MAAMYIDPRLKLQM KPHLQNGAT+EEIEGLIRVYADGH+ERPPIIPNV+CSTTAS+KGV
Sbjct: 1   MAAMYIDPRLKLQMAKPHLQNGATSEEIEGLIRVYADGHVERPPIIPNVACSTTASNKGV 60

Query: 61  TAKDVLIDNFTNTWARIYIPNSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLIDLTSKSKC 120
           TAKDVLID FTNTWARIYIPNSQRNLPLLVYFHGGGFCVGSAAWSCYHEFL++L SK+KC
Sbjct: 61  TAKDVLIDIFTNTWARIYIPNSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKAKC 120

Query: 121 VVLSVNYRLSPENRLPAAYEDGFNALMWLKQQAVTDPIEHKWWLGNCNPSKLFLAGDSAG 180
           V+LS+NYRLSPENRLPAAYEDGFNA+MWLKQQA+T+PIEHKWWLGNCNPSKLFLAGDSAG
Sbjct: 121 VILSINYRLSPENRLPAAYEDGFNAVMWLKQQAITNPIEHKWWLGNCNPSKLFLAGDSAG 180

Query: 181 ANIAYNIATRLGLNESTILKPLTLKGIILIQPFFGGESRTRSEKLAAQPSSASALTLAAA 240
           ANIAYN ATRLG NEST+LKP TLKGIILIQPFFGGESRT+SEKLAA PSS SALTL+A+
Sbjct: 181 ANIAYNTATRLGHNESTLLKPPTLKGIILIQPFFGGESRTKSEKLAATPSSTSALTLSAS 240

Query: 241 DTYWRLSLPPAVNRDHPWSNPLVNGGTKLKETKLPPLIVCISELDILMDRNLEFCAAMAG 300
           DTYWRLSLPP  NRDHPWSNPLVNGG KLKE KLPPL+VCISELDIL+DRNLEFCAAMA 
Sbjct: 241 DTYWRLSLPPTANRDHPWSNPLVNGGAKLKEMKLPPLMVCISELDILLDRNLEFCAAMAA 300

Query: 301 AGKKVERVILKGVGHGFQILNKDQISTLRTQEMMGSIQAFIQQ 344
           AGKKVERVILKGVGHGFQILNKDQIST RTQE+MGSI+AFI Q
Sbjct: 301 AGKKVERVILKGVGHGFQILNKDQISTQRTQELMGSIRAFIHQ 343

BLAST of Lag0000609 vs. NCBI nr
Match: KAG6607091.1 (putative carboxylesterase 17, partial [Cucurbita argyrosperma subsp. sororia] >KAG7036780.1 putative carboxylesterase 17, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 645.6 bits (1664), Expect = 2.4e-181
Identity = 307/343 (89.50%), Postives = 326/343 (95.04%), Query Frame = 0

Query: 1   MAAMYIDPRLKLQMPKPHLQNGATAEEIEGLIRVYADGHIERPPIIPNVSCSTTASDKGV 60
           MAAMYIDPRLKLQM KPHLQNG T+EEIEGLIRVYADGH+ERPPIIPNV+CSTTASDKGV
Sbjct: 1   MAAMYIDPRLKLQMAKPHLQNGGTSEEIEGLIRVYADGHVERPPIIPNVACSTTASDKGV 60

Query: 61  TAKDVLIDNFTNTWARIYIPNSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLIDLTSKSKC 120
           TAKDVLID FTNTWARIYIPNSQRNLPLLVYFHGGGFCVGSAAWSCYHEFL++L SK+KC
Sbjct: 61  TAKDVLIDIFTNTWARIYIPNSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKAKC 120

Query: 121 VVLSVNYRLSPENRLPAAYEDGFNALMWLKQQAVTDPIEHKWWLGNCNPSKLFLAGDSAG 180
           V+LS+NYRLSPENRLPAAY+DGFNALMWLKQQA+T+ IEHKWWLGNCNPSKLFLAGDSAG
Sbjct: 121 VILSINYRLSPENRLPAAYDDGFNALMWLKQQAITNAIEHKWWLGNCNPSKLFLAGDSAG 180

Query: 181 ANIAYNIATRLGLNESTILKPLTLKGIILIQPFFGGESRTRSEKLAAQPSSASALTLAAA 240
           ANIAYN ATRLG NEST+LKPLTLKGIILIQPFFGGESRT+SEKLAA PSS SALTL+A+
Sbjct: 181 ANIAYNTATRLGHNESTLLKPLTLKGIILIQPFFGGESRTKSEKLAATPSSTSALTLSAS 240

Query: 241 DTYWRLSLPPAVNRDHPWSNPLVNGGTKLKETKLPPLIVCISELDILMDRNLEFCAAMAG 300
           DTYWRLSLPP  NRDHPWSNPLVNG  KLKE KLPPL+V ISELDIL+DRNLEFCAAMAG
Sbjct: 241 DTYWRLSLPPTANRDHPWSNPLVNGSAKLKEMKLPPLMVSISELDILLDRNLEFCAAMAG 300

Query: 301 AGKKVERVILKGVGHGFQILNKDQISTLRTQEMMGSIQAFIQQ 344
           AGKKVERVILKGVGHGFQILNKDQIST RTQE+MGSI+AFI Q
Sbjct: 301 AGKKVERVILKGVGHGFQILNKDQISTQRTQELMGSIRAFIHQ 343

BLAST of Lag0000609 vs. NCBI nr
Match: XP_022155020.1 (probable carboxylesterase 17 [Momordica charantia])

HSP 1 Score: 619.4 bits (1596), Expect = 1.9e-173
Identity = 296/343 (86.30%), Postives = 317/343 (92.42%), Query Frame = 0

Query: 1   MAAMYIDPRLKLQMPKPHLQNGATAEEIEGLIRVYADGHIERPPIIPNVSCSTTASDKGV 60
           MAA+ IDPRLKLQMPK HLQ  +TAEEIEGLIRVYADGH+ERPPIIPNV CSTTAS+KGV
Sbjct: 6   MAAISIDPRLKLQMPKSHLQCASTAEEIEGLIRVYADGHVERPPIIPNVPCSTTASNKGV 65

Query: 61  TAKDVLIDNFTNTWARIYIPNSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLIDLTSKSKC 120
           +AKDVLIDNFTNTWARIYI NSQR LPLLVYFHGGGFCVGSAAWSCYHEFL+DL SK+KC
Sbjct: 66  SAKDVLIDNFTNTWARIYIANSQRKLPLLVYFHGGGFCVGSAAWSCYHEFLLDLASKAKC 125

Query: 121 VVLSVNYRLSPENRLPAAYEDGFNALMWLKQQAVTDPIEHKWWLGNCNPSKLFLAGDSAG 180
            +LS+NYRLSPENRLPAAYEDGFNALMWLKQQA+++P EHKWWLGNCNPS LFLAGDSAG
Sbjct: 126 AILSINYRLSPENRLPAAYEDGFNALMWLKQQAISEPNEHKWWLGNCNPSNLFLAGDSAG 185

Query: 181 ANIAYNIATRLGLNESTILKPLTLKGIILIQPFFGGESRTRSEKLAAQPSSASALTLAAA 240
           ANIAYNIATR GLNE   LKPLTLKG+ILIQPFFGGESRT+SEKLAAQPS+ SALTLA A
Sbjct: 186 ANIAYNIATRPGLNEPATLKPLTLKGVILIQPFFGGESRTKSEKLAAQPST-SALTLATA 245

Query: 241 DTYWRLSLPPAVNRDHPWSNPLVNGGTKLKETKLPPLIVCISELDILMDRNLEFCAAMAG 300
           DTYWRLSLPP  +RDHPWSNPLVNGGTKL++TKLPP +VCISELDIL+DRNLEFCAAMA 
Sbjct: 246 DTYWRLSLPPGASRDHPWSNPLVNGGTKLRDTKLPPSMVCISELDILVDRNLEFCAAMAA 305

Query: 301 AGKKVERVILKGVGHGFQILNKDQISTLRTQEMMGSIQAFIQQ 344
           AGKKVERVI KGVGHGFQILNKDQIS +RTQEMM SIQ FI Q
Sbjct: 306 AGKKVERVIFKGVGHGFQILNKDQISAIRTQEMMVSIQNFIHQ 347

BLAST of Lag0000609 vs. ExPASy Swiss-Prot
Match: Q9LFR7 (Probable carboxylesterase 17 OS=Arabidopsis thaliana OX=3702 GN=CXE17 PE=1 SV=1)

HSP 1 Score: 340.9 bits (873), Expect = 1.7e-92
Identity = 173/329 (52.58%), Postives = 222/329 (67.48%), Query Frame = 0

Query: 18  HLQNGATAEEIEGLIRVYADGHIERPPIIPNVSCSTTASDKGVTAKDVLIDNFTNTWARI 77
           H ++G   EEIEGLI+V+ DG +ERPPI+P VS +   S K  TA D+ + N  +TW R+
Sbjct: 22  HHRHGPVVEEIEGLIKVFNDGCVERPPIVPIVSPTIHPSSK-ATAFDIKLSN--DTWTRV 81

Query: 78  YIPNSQR-----NLPLLVYFHGGGFCVGSAAWSCYHEFLIDLTSKSKCVVLSVNYRLSPE 137
           YIP++        LPLLVYFHGGGFCVGSAAWSCYH+FL  L  K++CV++SVNYRL+PE
Sbjct: 82  YIPDAAAASPSVTLPLLVYFHGGGFCVGSAAWSCYHDFLTSLAVKARCVIVSVNYRLAPE 141

Query: 138 NRLPAAYEDGFNALMWLKQQAVTDPIEHKWWLGNCNPSKLFLAGDSAGANIAYNIATRLG 197
           +RLPAAY+DG N + WL +Q ++    +  WL  CN S +FLAGDSAGANIAY +A R+ 
Sbjct: 142 HRLPAAYDDGVNVVSWLVKQQISTGGGYPSWLSKCNLSNVFLAGDSAGANIAYQVAVRI- 201

Query: 198 LNESTILKPLTLKGIILIQPFFGGESRTRSEKLAAQPSSASALTLAAADTYWRLSLPPAV 257
           +        L LKGIILI PFFGGESRT SEK     + +SALTL+A+D YWRL+LP   
Sbjct: 202 MASGKYANTLHLKGIILIHPFFGGESRTSSEK-QQHHTKSSALTLSASDAYWRLALPRGA 261

Query: 258 NRDHPWSNPLVNGGTKLKETKLPPLIVCISELDILMDRNLEFCAAMAGAGKKVERVILKG 317
           +RDHPW NPL++        KLP  +V ++E DIL +RNLE C  M   GK+VE ++  G
Sbjct: 262 SRDHPWCNPLMSSA----GAKLPTTMVFMAEFDILKERNLEMCKVMRSHGKRVEGIVHGG 321

Query: 318 VGHGFQILNKDQISTLRTQEMMGSIQAFI 342
           VGH F IL+   +S  R  +MM  +  FI
Sbjct: 322 VGHAFHILDNSSVSRDRIHDMMCRLHNFI 341

BLAST of Lag0000609 vs. ExPASy Swiss-Prot
Match: Q9SX25 (Probable carboxylesterase 6 OS=Arabidopsis thaliana OX=3702 GN=CXE6 PE=2 SV=1)

HSP 1 Score: 314.7 bits (805), Expect = 1.3e-84
Identity = 160/326 (49.08%), Postives = 216/326 (66.26%), Query Frame = 0

Query: 21  NGATAEEIEGLIRVYADGHIERPPIIPNVSCSTTASDKGVTAKDVLIDNFTNTWARIYIP 80
           +G   +E+EGLI+VY DGH+ER  ++P V  S    + GVT  DV+ID  TN WAR+Y+P
Sbjct: 20  HGPVVDEVEGLIKVYKDGHVERSQLLPCVDPSLPL-ELGVTCSDVVIDKLTNVWARLYVP 79

Query: 81  -----NSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLIDLTSKSKCVVLSVNYRLSPENRL 140
                +S   LPL+VYFHGGGFCVGSA+W CYHEFL  L+++S+C+V+SVNYRL+PEN L
Sbjct: 80  MTTTKSSVSKLPLIVYFHGGGFCVGSASWLCYHEFLARLSARSRCLVMSVNYRLAPENPL 139

Query: 141 PAAYEDGFNALMWLKQQAVTDPIEHKWWLGNCNPSKLFLAGDSAGANIAYNIATRLGLNE 200
           PAAYEDG NA++WL  +A  D +    W   C+  ++FLAGDSAG NIA  +A RL   E
Sbjct: 140 PAAYEDGVNAILWL-NKARNDNL----WAKQCDFGRIFLAGDSAGGNIAQQVAARLASPE 199

Query: 201 STILKPLTLKGIILIQPFFGGESRTRSEKLAAQPSSASALTLAAADTYWRLSLPPAVNRD 260
              LK   ++G ILIQPF+ GE RT SE+      +A  LTLA++D +WR+SLP   NR+
Sbjct: 200 DLALK---IEGTILIQPFYSGEERTESERRVGNDKTA-VLTLASSDAWWRMSLPRGANRE 259

Query: 261 HPWSNPLVNGGTKLKETKLPPLIVCISELDILMDRNLEFCAAMAGAGKKVERVILKGVGH 320
           HP+  P+      +K + +   +VC++E+D+LMD N+E C    G    ++RV+ KGVGH
Sbjct: 260 HPYCKPV---KMIIKSSTVTRTLVCVAEMDLLMDSNMEMC---DGNEDVIKRVLHKGVGH 319

Query: 321 GFQILNKDQISTLRTQEMMGSIQAFI 342
            F IL K Q++   T EM+  I AFI
Sbjct: 320 AFHILGKSQLAHTTTLEMLCQIDAFI 329

BLAST of Lag0000609 vs. ExPASy Swiss-Prot
Match: Q9FG13 (Probable carboxylesterase 15 OS=Arabidopsis thaliana OX=3702 GN=CXE15 PE=2 SV=1)

HSP 1 Score: 197.6 bits (501), Expect = 2.3e-49
Identity = 113/299 (37.79%), Postives = 171/299 (57.19%), Query Frame = 0

Query: 25  AEEIEGLIRVYADGHIERPPIIPNVSCSTT-ASDKGVTAKDVLIDNFTNTWARIYIPNSQ 84
           AE+  GL+++ ++G + R   I  ++      +++ V  KD +     N   R+Y P S 
Sbjct: 11  AEDCMGLLQLLSNGTVLRSESIDLITQQIPFKNNQTVLFKDSIYHKPNNLHLRLYKPISA 70

Query: 85  RN---LPLLVYFHGGGFCVGSAAWSCYHEFLIDLTSKSKCVVLSVNYRLSPENRLPAAYE 144
            N   LP++V+FHGGGFC GS +W  +H F + L S    +V+S +YRL+PE+RLPAA+E
Sbjct: 71  SNRTALPVVVFFHGGGFCFGSRSWPHFHNFCLTLASSLNALVVSPDYRLAPEHRLPAAFE 130

Query: 145 DGFNALMWLKQQAVTDPIEHKWWLG-NCNPSKLFLAGDSAGANIAYNIATRLGLNESTIL 204
           D    L WL  QAV+D + H +  G + +  ++F+ GDS+G NIA+ +A R G + S  L
Sbjct: 131 DAEAVLTWLWDQAVSDGVNHWFEDGTDVDFDRVFVVGDSSGGNIAHQLAVRFG-SGSIEL 190

Query: 205 KPLTLKGIILIQPFFGGESRTRSEKLAAQPSSASALTLAAADTYWRLSLPPAVNRDHPWS 264
            P+ ++G +L+ PFFGGE RT SE     PS A  L+L   D +WRLSLP    RDH  +
Sbjct: 191 TPVRVRGYVLMGPFFGGEERTNSEN---GPSEA-LLSLDLLDKFWRLSLPNGATRDHHMA 250

Query: 265 NPLVNGGTKLKETKLPPLIVCISELDILMDRNLEFCAAMAG-AGKKVERVILKGVGHGF 318
           NP       L+   L P++V +   ++L DR  E+   +    GK+V+ +  +   HGF
Sbjct: 251 NPFGPTSPTLESISLEPMLVIVGGSELLRDRAKEYAYKLKKMGGKRVDYIEFENKEHGF 304

BLAST of Lag0000609 vs. ExPASy Swiss-Prot
Match: Q9LVB8 (Probable carboxylesterase 120 OS=Arabidopsis thaliana OX=3702 GN=CXE20 PE=2 SV=1)

HSP 1 Score: 172.9 bits (437), Expect = 6.1e-42
Identity = 105/307 (34.20%), Postives = 159/307 (51.79%), Query Frame = 0

Query: 37  DGHIERPPIIPNVSCSTTASDKG----VTAKDVLIDNFTNTWARIYIPNSQRN------- 96
           DG I R   + N  C+    D        +KD+ ++   +TW R+Y+P+S  N       
Sbjct: 21  DGSITRD--LSNFPCTAATPDPSPLNPAVSKDLPVNQLKSTWLRLYLPSSAVNEGNVSSQ 80

Query: 97  -LPLLVYFHGGGFCVGSAAWSCYHEFLIDLTSKSKCVVLSVNYRLSPENRLPAAYEDGFN 156
            LP++VY+HGGGF + S     +H+F  ++      +V+S +YRL+PE+RLPAAY+DG  
Sbjct: 81  KLPIVVYYHGGGFILCSVDMQLFHDFCSEVARDLNAIVVSPSYRLAPEHRLPAAYDDGVE 140

Query: 157 ALMWLKQQAVTDPIEHKWWLGNCNPSKLFLAGDSAGANIAYNIATRLGLNESTILKPLTL 216
           AL W+K        + +W   + + S +FL G SAG N+AYN+  R  ++  + L PL +
Sbjct: 141 ALDWIKTS------DDEWIKSHADFSNVFLMGTSAGGNLAYNVGLR-SVDSVSDLSPLQI 200

Query: 217 KGIILIQPFFGGESRTRSEKLAAQPSSASALTLAAADTYWRLSLPPAVNRDHPWSNPLVN 276
           +G+IL  PFFGGE R+ SE           +     D  W LSLP  V+RDH +SNP V 
Sbjct: 201 RGLILHHPFFGGEERSESEIRLMNDQVCPPI---VTDVMWDLSLPVGVDRDHEYSNPTVG 260

Query: 277 GGTKLKET--KLPPLIVCI-SELDILMDRNLEFCAAMAGAGKKVERVILKGVGHGFQILN 329
            G++  E   +L   ++ I  E D ++D   +    M   G +V      G  HG +I +
Sbjct: 261 DGSEKLEKIGRLRWKVMMIGGEDDPMIDLQKDVAKLMKKKGVEVVEHYTGGHVHGAEIRD 315

BLAST of Lag0000609 vs. ExPASy Swiss-Prot
Match: O64640 (Probable carboxylesterase 8 OS=Arabidopsis thaliana OX=3702 GN=CXE8 PE=2 SV=1)

HSP 1 Score: 167.5 bits (423), Expect = 2.6e-40
Identity = 99/282 (35.11%), Postives = 151/282 (53.55%), Query Frame = 0

Query: 62  AKDVLIDNFTNTWARIYIPNS---QRNLPLLVYFHGGGFCVGSAAWSCYHEFLIDLTSKS 121
           +KD+ ++   NT+ RI+ P +   +  LP+LVYFHGGGF + SAA + +HE    +  + 
Sbjct: 39  SKDIPLNQTNNTFIRIFKPRNIPPESKLPILVYFHGGGFILYSAASAPFHESCTKMADRL 98

Query: 122 KCVVLSVNYRLSPENRLPAAYEDGFNALMWLKQQAVTDPIE----HKWWLGNCNPSKLFL 181
           + ++LSV YRL+PE+RLPAAYED   A++WL+ QA   PI       W     + SK ++
Sbjct: 99  QTIILSVEYRLAPEHRLPAAYEDAVEAILWLRDQA-RGPINGGDCDTWLKDGVDFSKCYV 158

Query: 182 AGDSAGANIAYNIATRLGLNESTILKPLTLKGIILIQPFFGG------ESRTRSEKLAAQ 241
            G S+G NI YN+A R+     T L P+ ++G+I+ Q FFGG      ESR + +K+   
Sbjct: 159 MGSSSGGNIVYNVALRV---VDTDLSPVKIQGLIMNQAFFGGVEPSDSESRLKDDKICPL 218

Query: 242 PSSASALTLAAADTYWRLSLPPAVNRDHPWSNPLVNGGTKLKET--KLPPLIVCISELDI 301
           P         A    W L LP  V+RDH +SNP+ + G + K+   + P  ++     D 
Sbjct: 219 P---------ATHLLWSLCLPDGVDRDHVYSNPIKSSGPQEKDKMGRFPSTLINGYGGDP 278

Query: 302 LMDRNLEFCAAMAGAGKKVERVILKGVGHGFQILNKDQISTL 329
           L+DR       + G G  VE    K   H  ++ + ++   L
Sbjct: 279 LVDRQRHVAEMLKGRGVHVETRFDKDGFHACELFDGNKAKAL 307

BLAST of Lag0000609 vs. ExPASy TrEMBL
Match: A0A6J1G8X8 (probable carboxylesterase 17 OS=Cucurbita moschata OX=3662 GN=LOC111452001 PE=4 SV=1)

HSP 1 Score: 654.1 bits (1686), Expect = 3.3e-184
Identity = 309/343 (90.09%), Postives = 329/343 (95.92%), Query Frame = 0

Query: 1   MAAMYIDPRLKLQMPKPHLQNGATAEEIEGLIRVYADGHIERPPIIPNVSCSTTASDKGV 60
           MAAMYIDPRLKLQM KPHLQNG T+EEIEGLIRVYADGH+ERPPIIPNV+CSTTASDKGV
Sbjct: 6   MAAMYIDPRLKLQMAKPHLQNGGTSEEIEGLIRVYADGHVERPPIIPNVACSTTASDKGV 65

Query: 61  TAKDVLIDNFTNTWARIYIPNSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLIDLTSKSKC 120
           TAKDVLID FTNTWARIYIPNSQRNLPLLVYFHGGGFCVGSAAWSCYHEFL++L SK+KC
Sbjct: 66  TAKDVLIDIFTNTWARIYIPNSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKAKC 125

Query: 121 VVLSVNYRLSPENRLPAAYEDGFNALMWLKQQAVTDPIEHKWWLGNCNPSKLFLAGDSAG 180
           V+LS+NYRLSPENRLPAAY+DGFNALMWLKQQA+T+PIEHKWWLGNCNPSKLFLAGDSAG
Sbjct: 126 VILSINYRLSPENRLPAAYDDGFNALMWLKQQAITNPIEHKWWLGNCNPSKLFLAGDSAG 185

Query: 181 ANIAYNIATRLGLNESTILKPLTLKGIILIQPFFGGESRTRSEKLAAQPSSASALTLAAA 240
           ANI+YN ATR+G NEST+LKPLTLKGIILIQPFFGGESRT+SEKLAA PSS SALTL+AA
Sbjct: 186 ANISYNTATRIGHNESTLLKPLTLKGIILIQPFFGGESRTKSEKLAATPSSTSALTLSAA 245

Query: 241 DTYWRLSLPPAVNRDHPWSNPLVNGGTKLKETKLPPLIVCISELDILMDRNLEFCAAMAG 300
           DTYWRLSLPP  NRDHPWSNPLVNGG KLKE KLPPL+VCISELDIL+DRNLEFCAAMAG
Sbjct: 246 DTYWRLSLPPTANRDHPWSNPLVNGGAKLKEMKLPPLMVCISELDILLDRNLEFCAAMAG 305

Query: 301 AGKKVERVILKGVGHGFQILNKDQISTLRTQEMMGSIQAFIQQ 344
           AGKKVERVILKGVGHGFQILNKDQIST RTQE+MGSI+AFI Q
Sbjct: 306 AGKKVERVILKGVGHGFQILNKDQISTQRTQELMGSIRAFIHQ 348

BLAST of Lag0000609 vs. ExPASy TrEMBL
Match: A0A6J1KCZ3 (probable carboxylesterase 17 OS=Cucurbita maxima OX=3661 GN=LOC111493192 PE=4 SV=1)

HSP 1 Score: 649.4 bits (1674), Expect = 8.2e-183
Identity = 308/343 (89.80%), Postives = 328/343 (95.63%), Query Frame = 0

Query: 1   MAAMYIDPRLKLQMPKPHLQNGATAEEIEGLIRVYADGHIERPPIIPNVSCSTTASDKGV 60
           MAAMYIDPRLKLQM KPHLQNGAT+EEIEGLIRVYADGH+ERPPIIPNV+CSTTAS+KGV
Sbjct: 1   MAAMYIDPRLKLQMAKPHLQNGATSEEIEGLIRVYADGHVERPPIIPNVACSTTASNKGV 60

Query: 61  TAKDVLIDNFTNTWARIYIPNSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLIDLTSKSKC 120
           TAKDVLID FTNTWARIYIPNSQRNLPLLVYFHGGGFCVGSAAWSCYHEFL++L SK+KC
Sbjct: 61  TAKDVLIDIFTNTWARIYIPNSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKAKC 120

Query: 121 VVLSVNYRLSPENRLPAAYEDGFNALMWLKQQAVTDPIEHKWWLGNCNPSKLFLAGDSAG 180
           V+LS+NYRLSPENRLPAAYEDGFNA+MWLKQQA+T+PIEHKWWLGNCNPSKLFLAGDSAG
Sbjct: 121 VILSINYRLSPENRLPAAYEDGFNAVMWLKQQAITNPIEHKWWLGNCNPSKLFLAGDSAG 180

Query: 181 ANIAYNIATRLGLNESTILKPLTLKGIILIQPFFGGESRTRSEKLAAQPSSASALTLAAA 240
           ANIAYN ATRLG NEST+LKP TLKGIILIQPFFGGESRT+SEKLAA PSS SALTL+A+
Sbjct: 181 ANIAYNTATRLGHNESTLLKPPTLKGIILIQPFFGGESRTKSEKLAATPSSTSALTLSAS 240

Query: 241 DTYWRLSLPPAVNRDHPWSNPLVNGGTKLKETKLPPLIVCISELDILMDRNLEFCAAMAG 300
           DTYWRLSLPP  NRDHPWSNPLVNGG KLKE KLPPL+VCISELDIL+DRNLEFCAAMA 
Sbjct: 241 DTYWRLSLPPTANRDHPWSNPLVNGGAKLKEMKLPPLMVCISELDILLDRNLEFCAAMAA 300

Query: 301 AGKKVERVILKGVGHGFQILNKDQISTLRTQEMMGSIQAFIQQ 344
           AGKKVERVILKGVGHGFQILNKDQIST RTQE+MGSI+AFI Q
Sbjct: 301 AGKKVERVILKGVGHGFQILNKDQISTQRTQELMGSIRAFIHQ 343

BLAST of Lag0000609 vs. ExPASy TrEMBL
Match: A0A6J1DL90 (probable carboxylesterase 17 OS=Momordica charantia OX=3673 GN=LOC111022166 PE=4 SV=1)

HSP 1 Score: 619.4 bits (1596), Expect = 9.1e-174
Identity = 296/343 (86.30%), Postives = 317/343 (92.42%), Query Frame = 0

Query: 1   MAAMYIDPRLKLQMPKPHLQNGATAEEIEGLIRVYADGHIERPPIIPNVSCSTTASDKGV 60
           MAA+ IDPRLKLQMPK HLQ  +TAEEIEGLIRVYADGH+ERPPIIPNV CSTTAS+KGV
Sbjct: 6   MAAISIDPRLKLQMPKSHLQCASTAEEIEGLIRVYADGHVERPPIIPNVPCSTTASNKGV 65

Query: 61  TAKDVLIDNFTNTWARIYIPNSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLIDLTSKSKC 120
           +AKDVLIDNFTNTWARIYI NSQR LPLLVYFHGGGFCVGSAAWSCYHEFL+DL SK+KC
Sbjct: 66  SAKDVLIDNFTNTWARIYIANSQRKLPLLVYFHGGGFCVGSAAWSCYHEFLLDLASKAKC 125

Query: 121 VVLSVNYRLSPENRLPAAYEDGFNALMWLKQQAVTDPIEHKWWLGNCNPSKLFLAGDSAG 180
            +LS+NYRLSPENRLPAAYEDGFNALMWLKQQA+++P EHKWWLGNCNPS LFLAGDSAG
Sbjct: 126 AILSINYRLSPENRLPAAYEDGFNALMWLKQQAISEPNEHKWWLGNCNPSNLFLAGDSAG 185

Query: 181 ANIAYNIATRLGLNESTILKPLTLKGIILIQPFFGGESRTRSEKLAAQPSSASALTLAAA 240
           ANIAYNIATR GLNE   LKPLTLKG+ILIQPFFGGESRT+SEKLAAQPS+ SALTLA A
Sbjct: 186 ANIAYNIATRPGLNEPATLKPLTLKGVILIQPFFGGESRTKSEKLAAQPST-SALTLATA 245

Query: 241 DTYWRLSLPPAVNRDHPWSNPLVNGGTKLKETKLPPLIVCISELDILMDRNLEFCAAMAG 300
           DTYWRLSLPP  +RDHPWSNPLVNGGTKL++TKLPP +VCISELDIL+DRNLEFCAAMA 
Sbjct: 246 DTYWRLSLPPGASRDHPWSNPLVNGGTKLRDTKLPPSMVCISELDILVDRNLEFCAAMAA 305

Query: 301 AGKKVERVILKGVGHGFQILNKDQISTLRTQEMMGSIQAFIQQ 344
           AGKKVERVI KGVGHGFQILNKDQIS +RTQEMM SIQ FI Q
Sbjct: 306 AGKKVERVIFKGVGHGFQILNKDQISAIRTQEMMVSIQNFIHQ 347

BLAST of Lag0000609 vs. ExPASy TrEMBL
Match: A0A2N9EQ59 (Abhydrolase_3 domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS4850 PE=4 SV=1)

HSP 1 Score: 475.3 bits (1222), Expect = 2.1e-130
Identity = 227/346 (65.61%), Postives = 272/346 (78.61%), Query Frame = 0

Query: 1   MAAMYIDPRLKLQMPKPHLQNGATAEEIEGLIRVYADGHIERPPIIPNVSCSTTASDKGV 60
           MAA+ +DPR KLQ+ K H Q+G   EEIEGLI+V  DGH+ERPPI+P V C T A ++GV
Sbjct: 1   MAAISLDPRFKLQVGKNHHQHGGVVEEIEGLIKVCMDGHVERPPIVPIVPC-TMALERGV 60

Query: 61  TAKDVLIDNFTNTWARIYIPNSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLIDLTSKSKC 120
           TA DV+ID  T+ WARIY+P+    LPLLVYFHGGGFCVGSAAWSCY+EFL +L SK+ C
Sbjct: 61  TANDVVIDKCTSLWARIYVPSCSGKLPLLVYFHGGGFCVGSAAWSCYNEFLSNLASKANC 120

Query: 121 VVLSVNYRLSPENRLPAAYEDGFNALMWLKQQAVTDPIEHKWWLGNCNPSKLFLAGDSAG 180
           V++SVNYRL+PENRLPAAYEDGFN LMW+KQQA+    EHKWWL +CN + +FL GDS+G
Sbjct: 121 VIISVNYRLAPENRLPAAYEDGFNTLMWVKQQALNGSNEHKWWLRHCNLTSMFLGGDSSG 180

Query: 181 ANIAYNIATRL---GLNESTILKPLTLKGIILIQPFFGGESRTRSEKLAAQPSSASALTL 240
           ANIAYN+ATRL   G +ESTI++PL LKG ILIQPFFGGE+RT SEK   QP++ S LTL
Sbjct: 181 ANIAYNVATRLGSSGTSESTIIRPLCLKGTILIQPFFGGEARTWSEKYTNQPAN-SGLTL 240

Query: 241 AAADTYWRLSLPPAVNRDHPWSNPLVNGGTKLKETKLPPLIVCISELDILMDRNLEFCAA 300
             +DTYWRLSLP   NRD+PW NPL NG  KL+E +LP ++VC+SE DIL DRNLEFC A
Sbjct: 241 LVSDTYWRLSLPIGTNRDNPWCNPLANGMAKLREFRLPSIMVCVSETDILKDRNLEFCTA 300

Query: 301 MAGAGKKVERVILKGVGHGFQILNKDQISTLRTQEMMGSIQAFIQQ 344
           +  AGK+VE VI KGVGH FQIL+  Q+S  RT EMM  I+AFI +
Sbjct: 301 LTNAGKRVETVIYKGVGHAFQILHNSQLSQPRTHEMMAHIKAFINE 344

BLAST of Lag0000609 vs. ExPASy TrEMBL
Match: F6HSF1 (Abhydrolase_3 domain-containing protein OS=Vitis vinifera OX=29760 GN=VIT_14s0006g03180 PE=4 SV=1)

HSP 1 Score: 471.9 bits (1213), Expect = 2.3e-129
Identity = 225/344 (65.41%), Postives = 269/344 (78.20%), Query Frame = 0

Query: 1   MAAMYIDPRLKLQMPKPHLQNGATAEEIEGLIRVYADGHIERPPIIPNVSCSTTASDKGV 60
           MAA+ +DPRL LQM K   QNG   E++EGLIRVY DGH+ERP I+PNV C T A + GV
Sbjct: 1   MAAISLDPRLSLQMGKNLYQNGVVVEKVEGLIRVYNDGHVERPAIVPNVPC-TVALELGV 60

Query: 61  TAKDVLIDNFTNTWARIYIPNSQR-NLPLLVYFHGGGFCVGSAAWSCYHEFLIDLTSKSK 120
           T KDV+I+ ++N WAR Y+P+     LPLLVYFHGGGFCVGSAAW+CYH FL DL SK+ 
Sbjct: 61  TVKDVVIEKYSNLWARFYVPSCPAGKLPLLVYFHGGGFCVGSAAWNCYHGFLADLASKAG 120

Query: 121 CVVLSVNYRLSPENRLPAAYEDGFNALMWLKQQAVTDPIEHKWWLGNCNPSKLFLAGDSA 180
           C+++SVNYRL+PENRLPAAYEDGFNA+MW+K QA+    E KWWL  CN S LFL GDSA
Sbjct: 121 CLIMSVNYRLAPENRLPAAYEDGFNAVMWVKNQALNGAGEQKWWLSRCNLSSLFLTGDSA 180

Query: 181 GANIAYNIATRLGLNESTILKPLTLKGIILIQPFFGGESRTRSEKLAAQPSSASALTLAA 240
           GANIAYN+ATRLG +++T LKPL+LKG ILIQPFFGGE+RT SE  + QP + SALTL+A
Sbjct: 181 GANIAYNVATRLGSSDTTFLKPLSLKGTILIQPFFGGEARTGSENHSTQPPN-SALTLSA 240

Query: 241 ADTYWRLSLPPAVNRDHPWSNPLVNGGTKLKETKLPPLIVCISELDILMDRNLEFCAAMA 300
           +DTYWRLSLP   NRDHP  NPL NG TKL+  +LPP +VCIS+ DIL DRNL+FC AMA
Sbjct: 241 SDTYWRLSLPLGANRDHPCCNPLANGSTKLRTLQLPPTMVCISDTDILKDRNLQFCTAMA 300

Query: 301 GAGKKVERVILKGVGHGFQILNKDQISTLRTQEMMGSIQAFIQQ 344
            AGK++E VI KGVGH FQ+L    +S  RT+EM+  I+AFI Q
Sbjct: 301 NAGKRLETVIYKGVGHAFQVLQNSDLSQPRTKEMISHIRAFITQ 342

BLAST of Lag0000609 vs. TAIR 10
Match: AT5G16080.1 (carboxyesterase 17 )

HSP 1 Score: 340.9 bits (873), Expect = 1.2e-93
Identity = 173/329 (52.58%), Postives = 222/329 (67.48%), Query Frame = 0

Query: 18  HLQNGATAEEIEGLIRVYADGHIERPPIIPNVSCSTTASDKGVTAKDVLIDNFTNTWARI 77
           H ++G   EEIEGLI+V+ DG +ERPPI+P VS +   S K  TA D+ + N  +TW R+
Sbjct: 22  HHRHGPVVEEIEGLIKVFNDGCVERPPIVPIVSPTIHPSSK-ATAFDIKLSN--DTWTRV 81

Query: 78  YIPNSQR-----NLPLLVYFHGGGFCVGSAAWSCYHEFLIDLTSKSKCVVLSVNYRLSPE 137
           YIP++        LPLLVYFHGGGFCVGSAAWSCYH+FL  L  K++CV++SVNYRL+PE
Sbjct: 82  YIPDAAAASPSVTLPLLVYFHGGGFCVGSAAWSCYHDFLTSLAVKARCVIVSVNYRLAPE 141

Query: 138 NRLPAAYEDGFNALMWLKQQAVTDPIEHKWWLGNCNPSKLFLAGDSAGANIAYNIATRLG 197
           +RLPAAY+DG N + WL +Q ++    +  WL  CN S +FLAGDSAGANIAY +A R+ 
Sbjct: 142 HRLPAAYDDGVNVVSWLVKQQISTGGGYPSWLSKCNLSNVFLAGDSAGANIAYQVAVRI- 201

Query: 198 LNESTILKPLTLKGIILIQPFFGGESRTRSEKLAAQPSSASALTLAAADTYWRLSLPPAV 257
           +        L LKGIILI PFFGGESRT SEK     + +SALTL+A+D YWRL+LP   
Sbjct: 202 MASGKYANTLHLKGIILIHPFFGGESRTSSEK-QQHHTKSSALTLSASDAYWRLALPRGA 261

Query: 258 NRDHPWSNPLVNGGTKLKETKLPPLIVCISELDILMDRNLEFCAAMAGAGKKVERVILKG 317
           +RDHPW NPL++        KLP  +V ++E DIL +RNLE C  M   GK+VE ++  G
Sbjct: 262 SRDHPWCNPLMSSA----GAKLPTTMVFMAEFDILKERNLEMCKVMRSHGKRVEGIVHGG 321

Query: 318 VGHGFQILNKDQISTLRTQEMMGSIQAFI 342
           VGH F IL+   +S  R  +MM  +  FI
Sbjct: 322 VGHAFHILDNSSVSRDRIHDMMCRLHNFI 341

BLAST of Lag0000609 vs. TAIR 10
Match: AT1G68620.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 314.7 bits (805), Expect = 9.2e-86
Identity = 160/326 (49.08%), Postives = 216/326 (66.26%), Query Frame = 0

Query: 21  NGATAEEIEGLIRVYADGHIERPPIIPNVSCSTTASDKGVTAKDVLIDNFTNTWARIYIP 80
           +G   +E+EGLI+VY DGH+ER  ++P V  S    + GVT  DV+ID  TN WAR+Y+P
Sbjct: 20  HGPVVDEVEGLIKVYKDGHVERSQLLPCVDPSLPL-ELGVTCSDVVIDKLTNVWARLYVP 79

Query: 81  -----NSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLIDLTSKSKCVVLSVNYRLSPENRL 140
                +S   LPL+VYFHGGGFCVGSA+W CYHEFL  L+++S+C+V+SVNYRL+PEN L
Sbjct: 80  MTTTKSSVSKLPLIVYFHGGGFCVGSASWLCYHEFLARLSARSRCLVMSVNYRLAPENPL 139

Query: 141 PAAYEDGFNALMWLKQQAVTDPIEHKWWLGNCNPSKLFLAGDSAGANIAYNIATRLGLNE 200
           PAAYEDG NA++WL  +A  D +    W   C+  ++FLAGDSAG NIA  +A RL   E
Sbjct: 140 PAAYEDGVNAILWL-NKARNDNL----WAKQCDFGRIFLAGDSAGGNIAQQVAARLASPE 199

Query: 201 STILKPLTLKGIILIQPFFGGESRTRSEKLAAQPSSASALTLAAADTYWRLSLPPAVNRD 260
              LK   ++G ILIQPF+ GE RT SE+      +A  LTLA++D +WR+SLP   NR+
Sbjct: 200 DLALK---IEGTILIQPFYSGEERTESERRVGNDKTA-VLTLASSDAWWRMSLPRGANRE 259

Query: 261 HPWSNPLVNGGTKLKETKLPPLIVCISELDILMDRNLEFCAAMAGAGKKVERVILKGVGH 320
           HP+  P+      +K + +   +VC++E+D+LMD N+E C    G    ++RV+ KGVGH
Sbjct: 260 HPYCKPV---KMIIKSSTVTRTLVCVAEMDLLMDSNMEMC---DGNEDVIKRVLHKGVGH 319

Query: 321 GFQILNKDQISTLRTQEMMGSIQAFI 342
            F IL K Q++   T EM+  I AFI
Sbjct: 320 AFHILGKSQLAHTTTLEMLCQIDAFI 329

BLAST of Lag0000609 vs. TAIR 10
Match: AT5G06570.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 197.6 bits (501), Expect = 1.6e-50
Identity = 113/299 (37.79%), Postives = 171/299 (57.19%), Query Frame = 0

Query: 25  AEEIEGLIRVYADGHIERPPIIPNVSCSTT-ASDKGVTAKDVLIDNFTNTWARIYIPNSQ 84
           AE+  GL+++ ++G + R   I  ++      +++ V  KD +     N   R+Y P S 
Sbjct: 11  AEDCMGLLQLLSNGTVLRSESIDLITQQIPFKNNQTVLFKDSIYHKPNNLHLRLYKPISA 70

Query: 85  RN---LPLLVYFHGGGFCVGSAAWSCYHEFLIDLTSKSKCVVLSVNYRLSPENRLPAAYE 144
            N   LP++V+FHGGGFC GS +W  +H F + L S    +V+S +YRL+PE+RLPAA+E
Sbjct: 71  SNRTALPVVVFFHGGGFCFGSRSWPHFHNFCLTLASSLNALVVSPDYRLAPEHRLPAAFE 130

Query: 145 DGFNALMWLKQQAVTDPIEHKWWLG-NCNPSKLFLAGDSAGANIAYNIATRLGLNESTIL 204
           D    L WL  QAV+D + H +  G + +  ++F+ GDS+G NIA+ +A R G + S  L
Sbjct: 131 DAEAVLTWLWDQAVSDGVNHWFEDGTDVDFDRVFVVGDSSGGNIAHQLAVRFG-SGSIEL 190

Query: 205 KPLTLKGIILIQPFFGGESRTRSEKLAAQPSSASALTLAAADTYWRLSLPPAVNRDHPWS 264
            P+ ++G +L+ PFFGGE RT SE     PS A  L+L   D +WRLSLP    RDH  +
Sbjct: 191 TPVRVRGYVLMGPFFGGEERTNSEN---GPSEA-LLSLDLLDKFWRLSLPNGATRDHHMA 250

Query: 265 NPLVNGGTKLKETKLPPLIVCISELDILMDRNLEFCAAMAG-AGKKVERVILKGVGHGF 318
           NP       L+   L P++V +   ++L DR  E+   +    GK+V+ +  +   HGF
Sbjct: 251 NPFGPTSPTLESISLEPMLVIVGGSELLRDRAKEYAYKLKKMGGKRVDYIEFENKEHGF 304

BLAST of Lag0000609 vs. TAIR 10
Match: AT5G06570.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 197.6 bits (501), Expect = 1.6e-50
Identity = 113/299 (37.79%), Postives = 171/299 (57.19%), Query Frame = 0

Query: 25  AEEIEGLIRVYADGHIERPPIIPNVSCSTT-ASDKGVTAKDVLIDNFTNTWARIYIPNSQ 84
           AE+  GL+++ ++G + R   I  ++      +++ V  KD +     N   R+Y P S 
Sbjct: 11  AEDCMGLLQLLSNGTVLRSESIDLITQQIPFKNNQTVLFKDSIYHKPNNLHLRLYKPISA 70

Query: 85  RN---LPLLVYFHGGGFCVGSAAWSCYHEFLIDLTSKSKCVVLSVNYRLSPENRLPAAYE 144
            N   LP++V+FHGGGFC GS +W  +H F + L S    +V+S +YRL+PE+RLPAA+E
Sbjct: 71  SNRTALPVVVFFHGGGFCFGSRSWPHFHNFCLTLASSLNALVVSPDYRLAPEHRLPAAFE 130

Query: 145 DGFNALMWLKQQAVTDPIEHKWWLG-NCNPSKLFLAGDSAGANIAYNIATRLGLNESTIL 204
           D    L WL  QAV+D + H +  G + +  ++F+ GDS+G NIA+ +A R G + S  L
Sbjct: 131 DAEAVLTWLWDQAVSDGVNHWFEDGTDVDFDRVFVVGDSSGGNIAHQLAVRFG-SGSIEL 190

Query: 205 KPLTLKGIILIQPFFGGESRTRSEKLAAQPSSASALTLAAADTYWRLSLPPAVNRDHPWS 264
            P+ ++G +L+ PFFGGE RT SE     PS A  L+L   D +WRLSLP    RDH  +
Sbjct: 191 TPVRVRGYVLMGPFFGGEERTNSEN---GPSEA-LLSLDLLDKFWRLSLPNGATRDHHMA 250

Query: 265 NPLVNGGTKLKETKLPPLIVCISELDILMDRNLEFCAAMAG-AGKKVERVILKGVGHGF 318
           NP       L+   L P++V +   ++L DR  E+   +    GK+V+ +  +   HGF
Sbjct: 251 NPFGPTSPTLESISLEPMLVIVGGSELLRDRAKEYAYKLKKMGGKRVDYIEFENKEHGF 304

BLAST of Lag0000609 vs. TAIR 10
Match: AT5G62180.1 (carboxyesterase 20 )

HSP 1 Score: 172.9 bits (437), Expect = 4.3e-43
Identity = 105/307 (34.20%), Postives = 159/307 (51.79%), Query Frame = 0

Query: 37  DGHIERPPIIPNVSCSTTASDKG----VTAKDVLIDNFTNTWARIYIPNSQRN------- 96
           DG I R   + N  C+    D        +KD+ ++   +TW R+Y+P+S  N       
Sbjct: 21  DGSITRD--LSNFPCTAATPDPSPLNPAVSKDLPVNQLKSTWLRLYLPSSAVNEGNVSSQ 80

Query: 97  -LPLLVYFHGGGFCVGSAAWSCYHEFLIDLTSKSKCVVLSVNYRLSPENRLPAAYEDGFN 156
            LP++VY+HGGGF + S     +H+F  ++      +V+S +YRL+PE+RLPAAY+DG  
Sbjct: 81  KLPIVVYYHGGGFILCSVDMQLFHDFCSEVARDLNAIVVSPSYRLAPEHRLPAAYDDGVE 140

Query: 157 ALMWLKQQAVTDPIEHKWWLGNCNPSKLFLAGDSAGANIAYNIATRLGLNESTILKPLTL 216
           AL W+K        + +W   + + S +FL G SAG N+AYN+  R  ++  + L PL +
Sbjct: 141 ALDWIKTS------DDEWIKSHADFSNVFLMGTSAGGNLAYNVGLR-SVDSVSDLSPLQI 200

Query: 217 KGIILIQPFFGGESRTRSEKLAAQPSSASALTLAAADTYWRLSLPPAVNRDHPWSNPLVN 276
           +G+IL  PFFGGE R+ SE           +     D  W LSLP  V+RDH +SNP V 
Sbjct: 201 RGLILHHPFFGGEERSESEIRLMNDQVCPPI---VTDVMWDLSLPVGVDRDHEYSNPTVG 260

Query: 277 GGTKLKET--KLPPLIVCI-SELDILMDRNLEFCAAMAGAGKKVERVILKGVGHGFQILN 329
            G++  E   +L   ++ I  E D ++D   +    M   G +V      G  HG +I +
Sbjct: 261 DGSEKLEKIGRLRWKVMMIGGEDDPMIDLQKDVAKLMKKKGVEVVEHYTGGHVHGAEIRD 315