Lag0000488 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0000488
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionInsulinase (Peptidase family M16) protein
Locationchr4: 8666516 .. 8667959 (-)
RNA-Seq ExpressionLag0000488
SyntenyLag0000488
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTGTGGGTCTTCACTTTCTCTCTCGACGGTGACTGAAAGCTCTTCATATTCGACGCAGTTTGACTTGATCTCTTCCCCGTCAATCACTGTGCTCTTCATCTCCAGCACGACGTGGGTTTTTTTTTGTGGGTTCAAAGTGTAGATGTGTGTGGCTATACTTCGTGGGTAGCAACCTGTGATGGTCGTGGCTGTTCGTGCTCAGATCTGAGGTCGTGGTGGTCGTGGGTAGCAAATTTGTTGTGGTCGTGGCTGTTCTGGCACTTTGGTTTGGTGTTTTGCTCGTTTTGGGGTTTGGCATTTTATTGGCTTTAGTTTAATAATTTTGAGTTTGTTTCTGACTCACCTATTCTTATGGGGTTTCTTCGATTTGGCTTCCTCTTTGTCGATTGAAGCTTGAAAAACTCCTTTCATTTTAGTTTCCAGCAGGTCATTGATGATTGAAGGATTGACGATGAAACACTGCGTTCTTGATTTCGGAACGACCAAAAATTTCTCTCCCAACATTTTCGAACTTAAACAGACCGAGAAAATCATCTCTGTAAGCTTTAGCTCCCCCCCCCCCCACATTGGTCGAGGATGAAACTTTTGTATTTGTTTTGTTTCAGGTTGAGTACTTTAGACATTTCCTTAAGCATGTCAAGTAGATTGCATATCAACTATTTTTGTTAAACTGGACTCTGCAATTCTTTTTGTTTTTCCACTTCTTTGTTCTTGAAATCATTTAGTAGATAAATTTGAAATCATTTAGTAGACAAATCTCATTGTAGACCAGAGCACAAAATTTTAAGGTAGTTCTTGTTATATGCTTGCTAAGTTTATTCTCTCTTTTTTGTTATTTTGATTAACAAAATAGTGATATTTTGAAATGTTGATTAGGCGATTGTTTTGCTAAATGATTGGCACTGATAACTGTTGTCTGGATTTTGTCATTTGCTAACTGAAAATTAGGAAAATATTTGCTTTGTTCAAGTCAACCTCGTGCCTTTGTCAATGTTGGTTTACCATTGATAAAGTGTTTTAGTGCTCTTGATTTCAATGCTGGTTAGAATTCAATGTCAATGCATTTTTTTTTCTTTCACATAGTTGATAACAATCTTAATGCAAGACATCTGGTTCTTACTTCTTTATTCGCTCTCCCTATTGAACTGTCAATTTAATTTATAATTATCCAGATGTACGTTATTGTTGTTTCACGAGCTGTTAAGCATGAGGATTTTGTTGAGCAAGTAAAGAAATTATTCACTAAGTTGTTGCAAAGGAGCCAACAATTTTTACTGATTTTGAGGTTAGGAATAATTCATGACGATGTTCCTTTGGCACAATTTGCTATTGCTTTTAACGGAACATCCTGGATAGATCTAGACTCCATCACTCTGATGGTTATGCAGGCTATGTTGAGTTCTTGGAATAAAAGTGCTGGAGCTGGAAAGCATATGGGGTGA

mRNA sequence

ATGTGTGGGTCTTCACTTTCTCTCTCGACGGTGACTGAAAGCTCTTCATATTCGACGCAGTTTGACTTGATCTCTTCCCCGTCAATCACTGTGCTCTTCATCTCCAGCACGACATCTGAGGTCGTGGTGGTCGTGGGTAGCAAATTTGTTGTGGTCGTGGCTGTTCTGGCACTTTGGTTTGGTGTTTTGCTCGTTTTGGGGTCATTGATGATTGAAGGATTGACGATGAAACACTGCGTTCTTGATTTCGGAACGACCAAAAATTTCTCTCCCAACATTTTCGAACTTAAACAGACCGAGAAAATCATCTCTATGTACGTTATTGTTGTTTCACGAGCTGTTAAGCATGAGGATTTTGTTGAGCAAGTAAAGAAATTATTCACTAAGTTGTTGCAAAGGAGCCAACAATTTTTACTGATTTTGAGGTTAGGAATAATTCATGACGATGTTCCTTTGGCACAATTTGCTATTGCTTTTAACGGAACATCCTGGATAGATCTAGACTCCATCACTCTGATGGTTATGCAGGCTATGTTGAGTTCTTGGAATAAAAGTGCTGGAGCTGGAAAGCATATGGGGTGA

Coding sequence (CDS)

ATGTGTGGGTCTTCACTTTCTCTCTCGACGGTGACTGAAAGCTCTTCATATTCGACGCAGTTTGACTTGATCTCTTCCCCGTCAATCACTGTGCTCTTCATCTCCAGCACGACATCTGAGGTCGTGGTGGTCGTGGGTAGCAAATTTGTTGTGGTCGTGGCTGTTCTGGCACTTTGGTTTGGTGTTTTGCTCGTTTTGGGGTCATTGATGATTGAAGGATTGACGATGAAACACTGCGTTCTTGATTTCGGAACGACCAAAAATTTCTCTCCCAACATTTTCGAACTTAAACAGACCGAGAAAATCATCTCTATGTACGTTATTGTTGTTTCACGAGCTGTTAAGCATGAGGATTTTGTTGAGCAAGTAAAGAAATTATTCACTAAGTTGTTGCAAAGGAGCCAACAATTTTTACTGATTTTGAGGTTAGGAATAATTCATGACGATGTTCCTTTGGCACAATTTGCTATTGCTTTTAACGGAACATCCTGGATAGATCTAGACTCCATCACTCTGATGGTTATGCAGGCTATGTTGAGTTCTTGGAATAAAAGTGCTGGAGCTGGAAAGCATATGGGGTGA

Protein sequence

MCGSSLSLSTVTESSSYSTQFDLISSPSITVLFISSTTSEVVVVVGSKFVVVVAVLALWFGVLLVLGSLMIEGLTMKHCVLDFGTTKNFSPNIFELKQTEKIISMYVIVVSRAVKHEDFVEQVKKLFTKLLQRSQQFLLILRLGIIHDDVPLAQFAIAFNGTSWIDLDSITLMVMQAMLSSWNKSAGAGKHMG
Homology
BLAST of Lag0000488 vs. NCBI nr
Match: XP_022144443.1 (probable mitochondrial-processing peptidase subunit beta, mitochondrial isoform X1 [Momordica charantia])

HSP 1 Score: 112.8 bits (281), Expect = 3.2e-21
Identity = 65/106 (61.32%), Postives = 68/106 (64.15%), Query Frame = 0

Query: 98  QTEKIISMYVIVVSRAVKHEDFVEQVKKLFTK----------LLQRSQQFLLILRLGIIH 157
           QT       VI  S AVKHEDFVEQVKKLFTK          L+ +         + II 
Sbjct: 268 QTHYTAPRMVIAASGAVKHEDFVEQVKKLFTKLSSEPTTASQLVAKEPAIFTGSEVRIID 327

Query: 158 DDVPLAQFAIAFNGTSWIDLDSITLMVMQAMLSSWNKSAGAGKHMG 194
           DDVPLAQFAIAFNG SW D DSI LMVMQAML SWNKSAG GKHMG
Sbjct: 328 DDVPLAQFAIAFNGASWTDPDSIALMVMQAMLGSWNKSAGGGKHMG 373

BLAST of Lag0000488 vs. NCBI nr
Match: XP_022144444.1 (probable mitochondrial-processing peptidase subunit beta, mitochondrial isoform X2 [Momordica charantia])

HSP 1 Score: 112.8 bits (281), Expect = 3.2e-21
Identity = 65/106 (61.32%), Postives = 68/106 (64.15%), Query Frame = 0

Query: 98  QTEKIISMYVIVVSRAVKHEDFVEQVKKLFTK----------LLQRSQQFLLILRLGIIH 157
           QT       VI  S AVKHEDFVEQVKKLFTK          L+ +         + II 
Sbjct: 268 QTHYTAPRMVIAASGAVKHEDFVEQVKKLFTKLSSEPTTASQLVAKEPAIFTGSEVRIID 327

Query: 158 DDVPLAQFAIAFNGTSWIDLDSITLMVMQAMLSSWNKSAGAGKHMG 194
           DDVPLAQFAIAFNG SW D DSI LMVMQAML SWNKSAG GKHMG
Sbjct: 328 DDVPLAQFAIAFNGASWTDPDSIALMVMQAMLGSWNKSAGGGKHMG 373

BLAST of Lag0000488 vs. NCBI nr
Match: TYK19532.1 (putative mitochondrial-processing peptidase subunit beta [Cucumis melo var. makuwa])

HSP 1 Score: 112.5 bits (280), Expect = 4.2e-21
Identity = 64/106 (60.38%), Postives = 68/106 (64.15%), Query Frame = 0

Query: 98  QTEKIISMYVIVVSRAVKHEDFVEQVKKLFTK----------LLQRSQQFLLILRLGIIH 157
           QT       VI  S AVKHEDFVEQVKKLFTK          L+ +         + I+ 
Sbjct: 297 QTHYTAPRMVIAASGAVKHEDFVEQVKKLFTKLSAEPTTAAQLVAKEPAIFTGSEVRIVD 356

Query: 158 DDVPLAQFAIAFNGTSWIDLDSITLMVMQAMLSSWNKSAGAGKHMG 194
           DDVPLAQFAIAFNG SW D DSI LMVMQAML SWNKSAG GKHMG
Sbjct: 357 DDVPLAQFAIAFNGASWTDPDSIALMVMQAMLGSWNKSAGGGKHMG 402

BLAST of Lag0000488 vs. NCBI nr
Match: KAG6607105.1 (putative mitochondrial-processing peptidase subunit beta, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 112.5 bits (280), Expect = 4.2e-21
Identity = 65/106 (61.32%), Postives = 68/106 (64.15%), Query Frame = 0

Query: 98  QTEKIISMYVIVVSRAVKHEDFVEQVKKLFTKLLQRSQQFLLIL----------RLGIIH 157
           QT       VI  S AVKHEDFVEQVKKLFTKL         ++           + II 
Sbjct: 313 QTHYTAPRMVIAASGAVKHEDFVEQVKKLFTKLSAEPTTATQLVAKEPASFTGSEVRIID 372

Query: 158 DDVPLAQFAIAFNGTSWIDLDSITLMVMQAMLSSWNKSAGAGKHMG 194
           DDVPLAQFAIAFNG SW D DSI LMVMQAML SWNKSAG GKHMG
Sbjct: 373 DDVPLAQFAIAFNGASWTDPDSIALMVMQAMLGSWNKSAGGGKHMG 418

BLAST of Lag0000488 vs. NCBI nr
Match: KAG7036796.1 (putative mitochondrial-processing peptidase subunit beta, mitochondrial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 112.5 bits (280), Expect = 4.2e-21
Identity = 65/106 (61.32%), Postives = 68/106 (64.15%), Query Frame = 0

Query: 98  QTEKIISMYVIVVSRAVKHEDFVEQVKKLFTKLLQRSQQFLLIL----------RLGIIH 157
           QT       VI  S AVKHEDFVEQVKKLFTKL         ++           + II 
Sbjct: 268 QTHYTAPRMVIAASGAVKHEDFVEQVKKLFTKLSAEPTTATQLVAKEPASFTGSEVRIID 327

Query: 158 DDVPLAQFAIAFNGTSWIDLDSITLMVMQAMLSSWNKSAGAGKHMG 194
           DDVPLAQFAIAFNG SW D DSI LMVMQAML SWNKSAG GKHMG
Sbjct: 328 DDVPLAQFAIAFNGASWTDPDSIALMVMQAMLGSWNKSAGGGKHMG 373

BLAST of Lag0000488 vs. ExPASy Swiss-Prot
Match: Q42290 (Probable mitochondrial-processing peptidase subunit beta, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=MPPbeta PE=1 SV=2)

HSP 1 Score: 95.5 bits (236), Expect = 6.9e-19
Identity = 53/106 (50.00%), Postives = 65/106 (61.32%), Query Frame = 0

Query: 98  QTEKIISMYVIVVSRAVKHEDFVEQVKKLFTKLLQRSQQFLLIL----------RLGIIH 157
           +T    S  VI  + AVKHE+ VEQVKKLFTKL         ++           + +I 
Sbjct: 271 KTHYTASRMVIAAAGAVKHEEVVEQVKKLFTKLSSDPTTTSQLVANEPASFTGSEVRMID 330

Query: 158 DDVPLAQFAIAFNGTSWIDLDSITLMVMQAMLSSWNKSAGAGKHMG 194
           DD+PLAQFA+AF G SW D DS+ LMVMQ ML SWNK+ G GKH+G
Sbjct: 331 DDLPLAQFAVAFEGASWTDPDSVALMVMQTMLGSWNKNVGGGKHVG 376

BLAST of Lag0000488 vs. ExPASy Swiss-Prot
Match: Q9P7X1 (Probable mitochondrial-processing peptidase subunit beta OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=qcr1 PE=2 SV=1)

HSP 1 Score: 48.5 bits (114), Expect = 9.7e-05
Identity = 30/97 (30.93%), Postives = 48/97 (49.48%), Query Frame = 0

Query: 107 VIVVSRAVKHEDFVEQVKKLFTKLLQRSQQFLLILRLGI-----------IHDDVPLAQF 166
           +I  + ++ HE+ V+  +K F  L   ++Q  L    G+             DD P A  
Sbjct: 205 IISSAGSISHEELVKLAEKYFGHLEPSAEQLSLGAPRGLKPRFVGSEIRARDDDSPTANI 264

Query: 167 AIAFNGTSWIDLDSITLMVMQAMLSSWNKSAGAGKHM 193
           AIA  G SW   D  T +VMQA++ +W+++ GA  H+
Sbjct: 265 AIAVEGMSWKHPDYFTALVMQAIIGNWDRAMGASPHL 301

BLAST of Lag0000488 vs. ExPASy Swiss-Prot
Match: P11913 (Mitochondrial-processing peptidase subunit beta OS=Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) OX=367110 GN=pep PE=1 SV=1)

HSP 1 Score: 48.1 bits (113), Expect = 1.3e-04
Identity = 32/98 (32.65%), Postives = 47/98 (47.96%), Query Frame = 0

Query: 107 VIVVSRAVKHEDFVEQVKKLFTKL-----------LQRSQQFLLILRLGIIHDDVPLAQF 166
           V+V +  V HE  VE   K F+KL           L + +   +   + I  D +P A  
Sbjct: 223 VLVGAGGVPHEQLVEMADKYFSKLPATAPVSSASILSKKKPDFIGSDIRIRDDTIPTANI 282

Query: 167 AIAFNGTSWIDLDSITLMVMQAMLSSWNKSAGAGKHMG 194
           AIA  G SW D D  T +V QA++ +++K+ G   H G
Sbjct: 283 AIAVEGVSWSDDDYFTGLVTQAIVGNYDKALGNAPHQG 320

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022144443.13.2e-2161.32probable mitochondrial-processing peptidase subunit beta, mitochondrial isoform ... [more]
XP_022144444.13.2e-2161.32probable mitochondrial-processing peptidase subunit beta, mitochondrial isoform ... [more]
TYK19532.14.2e-2160.38putative mitochondrial-processing peptidase subunit beta [Cucumis melo var. maku... [more]
KAG6607105.14.2e-2161.32putative mitochondrial-processing peptidase subunit beta, mitochondrial, partial... [more]
KAG7036796.14.2e-2161.32putative mitochondrial-processing peptidase subunit beta, mitochondrial [Cucurbi... [more]
Match NameE-valueIdentityDescription
Q422906.9e-1950.00Probable mitochondrial-processing peptidase subunit beta, mitochondrial OS=Arabi... [more]
Q9P7X19.7e-0530.93Probable mitochondrial-processing peptidase subunit beta OS=Schizosaccharomyces ... [more]
P119131.3e-0432.65Mitochondrial-processing peptidase subunit beta OS=Neurospora crassa (strain ATC... [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.30.830.10coord: 144..193
e-value: 8.3E-7
score: 30.6
NoneNo IPR availablePANTHERPTHR11851:SF204MITOCHONDRIAL-PROCESSING PEPTIDASE SUBUNIT BETAcoord: 106..193
NoneNo IPR availablePANTHERPTHR11851METALLOPROTEASEcoord: 106..193

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0000488.1Lag0000488.1mRNA