Lag0000458 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0000458 vs. NCBI nr
Match: MBA0699030.1 (hypothetical protein [Gossypium aridum])

HSP 1 Score: 100.9 bits (250), Expect = 1.4e-17
Identity = 63/156 (40.38%), Postives = 85/156 (54.49%), Query Frame = 0

Query: 47  HEIRSKNPHIVFITKTKSGSARMEKVKNDLNFDCGFCV-SNYWGG-------GDFNEILF 106
           H +R  NP +VF+ +TK   +R+E+++    +  G  V SN   G       GDFNEI +
Sbjct: 222 HMLRDSNPSVVFLIETKLKGSRLERIRCKCGYINGIDVDSNGRSGCLSMVVIGDFNEIAY 281

Query: 107 ADEKRGRAGRGQNLMEAFRLALDKCNLADLVAPRNVFTWMKRVRGGTTIAERLDRFLAND 166
             EKRG   R ++ M  FR  L  C +++L   R  FTW K       I ERLDR + ND
Sbjct: 282 MSEKRGGLVRHESQMRKFRDMLSDCVISNLRYTRQWFTWEKGHTTSNNIHERLDRAIVND 341

Query: 167 EFRSLFRNIGISHLNKHGSDHHPIVA-SMAKSGSTK 194
           E+ SLF N  +SHL+   SDH P++  S  KS STK
Sbjct: 342 EWWSLFSNYEVSHLSHFFSDHCPLLLNSKVKSLSTK 377

BLAST of Lag0000458 vs. NCBI nr
Match: MBA0667752.1 (hypothetical protein [Gossypium klotzschianum])

HSP 1 Score: 94.0 bits (232), Expect = 1.7e-15
Identity = 60/151 (39.74%), Postives = 82/151 (54.30%), Query Frame = 0

Query: 49  IRSKNPHIVFITKTKSGSARMEKVKNDLNFDCGF-----CVSNYWGGGDFNEILFADEKR 108
           +R  NP +VF+ +TK   +R+EK++      CG+        N    GDFNEI +  EKR
Sbjct: 235 LRDSNPSVVFLIETKLQGSRLEKIR----CKCGYINRIDVDPNGRIIGDFNEIAYMLEKR 294

Query: 109 GRAGRGQNLMEAFRLALDKCNLADLVAPRNVFTWMKRVRGGTTIAERLDRFLANDEFRSL 168
           G   R ++ M  FR  L  C +++L   R  FTW K       I ERLDR + NDE+ SL
Sbjct: 295 GGLVRQESQMRKFRDMLSDCVISNLRYTRQWFTWEKGHTASNNIHERLDRAVVNDEWWSL 354

Query: 169 FRNIGISHLNKHGSDHHPIVA-SMAKSGSTK 194
           F N  +SHL+   SDH P++  S  +S STK
Sbjct: 355 FSNYKVSHLSHFFSDHCPLLLNSKVESLSTK 381

BLAST of Lag0000458 vs. NCBI nr
Match: XP_030495196.1 (uncharacterized protein LOC115710989 [Cannabis sativa])

HSP 1 Score: 93.6 bits (231), Expect = 2.2e-15
Identity = 53/144 (36.81%), Postives = 80/144 (55.56%), Query Frame = 0

Query: 49  IRSKNPHIVFITKTKSGSARMEKVKNDLNFDCGFCVSNYWGGGDFNEILFADEKRGRAGR 108
           +R ++P ++F+ +T+ GS  + + +  L F  G   S  +  GDFNEIL   +K G + R
Sbjct: 24  VRQQSPQVLFLMETRLGSNAISRFRQSLQFKNGL-ESPRFLIGDFNEILSQSDKLGGSLR 83

Query: 109 GQNLMEAFRLALDKCNLADLVAPRNVFTWMKRVRGGTTIAERLDRFLANDEFRSLFRNIG 168
            ++ +EAFR  LD C+L DL    + FTW+K  +  TTI ERLD    N  + S+F    
Sbjct: 84  NESQIEAFRNTLDHCHLQDLPYEGDRFTWVKNRKAVTTIKERLDWVFTNHLWSSIFEVPT 143

Query: 169 ISHLNKHGSDHHPIVASMAKSGST 193
           +SHL+   SDH  I  ++    ST
Sbjct: 144 VSHLDFFSSDHRAIATTVTSLHST 166

BLAST of Lag0000458 vs. NCBI nr
Match: XP_021855734.1 (uncharacterized protein LOC110795065 [Spinacia oleracea])

HSP 1 Score: 92.8 bits (229), Expect = 3.8e-15
Identity = 49/128 (38.28%), Postives = 73/128 (57.03%), Query Frame = 0

Query: 81  GFCVSNYWGGGDFNEILFADEKRGRAGRGQNLMEAFRLALDKCNLADLVAPRNVFTWMKR 140
           G C+S     GDFNEI    EK G   RG+  M+AFR A+D+C + DL    ++FTW + 
Sbjct: 126 GECMSPLVLFGDFNEIAGPSEKEGGVSRGERQMDAFRAAMDQCRVRDLGYRGSIFTWQRG 185

Query: 141 VRGGTTIAERLDRFLANDEFRSLFRNIGISHLNKHGSDHHPIVASMAKSGSTKEVFKSEG 200
           +   T + ERLDRFLAN  + +LF N  + HL ++ SDH P++      G   +V +   
Sbjct: 186 LSTTTVVRERLDRFLANVAWSTLFPNAAVIHLPRYRSDHTPLLLKSDYLGGRHQVERLFK 245

Query: 201 IKALFAAE 209
            +AL+ ++
Sbjct: 246 FEALWLSK 253

BLAST of Lag0000458 vs. NCBI nr
Match: MBA0631995.1 (hypothetical protein [Gossypium davidsonii])

HSP 1 Score: 92.8 bits (229), Expect = 3.8e-15
Identity = 59/151 (39.07%), Postives = 82/151 (54.30%), Query Frame = 0

Query: 49  IRSKNPHIVFITKTKSGSARMEKVKNDLNFDCGF-----CVSNYWGGGDFNEILFADEKR 108
           +R  NP +VF+ +TK   +R+E+++      CG+        N    GDFNEI +  EKR
Sbjct: 235 LRDSNPSVVFLIETKLQGSRLERIR----CKCGYINRIDVDPNGRIIGDFNEIAYMLEKR 294

Query: 109 GRAGRGQNLMEAFRLALDKCNLADLVAPRNVFTWMKRVRGGTTIAERLDRFLANDEFRSL 168
           G   R ++ M  FR  L  C +++L   R  FTW K       I ERLDR + NDE+ SL
Sbjct: 295 GGLVRQESQMRKFRDMLSDCVISNLRYTRQWFTWEKGHTASNNIHERLDRAVVNDEWWSL 354

Query: 169 FRNIGISHLNKHGSDHHPIVA-SMAKSGSTK 194
           F N  +SHL+   SDH P++  S  +S STK
Sbjct: 355 FSNYKVSHLSHFFSDHCPLLLNSKVESLSTK 381

BLAST of Lag0000458 vs. ExPASy TrEMBL
Match: A0A7J8YIC1 (DUF4283 domain-containing protein OS=Gossypium aridum OX=34290 GN=Goari_000703 PE=4 SV=1)

HSP 1 Score: 100.9 bits (250), Expect = 6.8e-18
Identity = 63/156 (40.38%), Postives = 85/156 (54.49%), Query Frame = 0

Query: 47  HEIRSKNPHIVFITKTKSGSARMEKVKNDLNFDCGFCV-SNYWGG-------GDFNEILF 106
           H +R  NP +VF+ +TK   +R+E+++    +  G  V SN   G       GDFNEI +
Sbjct: 222 HMLRDSNPSVVFLIETKLKGSRLERIRCKCGYINGIDVDSNGRSGCLSMVVIGDFNEIAY 281

Query: 107 ADEKRGRAGRGQNLMEAFRLALDKCNLADLVAPRNVFTWMKRVRGGTTIAERLDRFLAND 166
             EKRG   R ++ M  FR  L  C +++L   R  FTW K       I ERLDR + ND
Sbjct: 282 MSEKRGGLVRHESQMRKFRDMLSDCVISNLRYTRQWFTWEKGHTTSNNIHERLDRAIVND 341

Query: 167 EFRSLFRNIGISHLNKHGSDHHPIVA-SMAKSGSTK 194
           E+ SLF N  +SHL+   SDH P++  S  KS STK
Sbjct: 342 EWWSLFSNYEVSHLSHFFSDHCPLLLNSKVKSLSTK 377

BLAST of Lag0000458 vs. ExPASy TrEMBL
Match: A0A7J8VYU1 (Uncharacterized protein OS=Gossypium klotzschianum OX=34286 GN=Goklo_000795 PE=4 SV=1)

HSP 1 Score: 94.0 bits (232), Expect = 8.3e-16
Identity = 60/151 (39.74%), Postives = 82/151 (54.30%), Query Frame = 0

Query: 49  IRSKNPHIVFITKTKSGSARMEKVKNDLNFDCGF-----CVSNYWGGGDFNEILFADEKR 108
           +R  NP +VF+ +TK   +R+EK++      CG+        N    GDFNEI +  EKR
Sbjct: 235 LRDSNPSVVFLIETKLQGSRLEKIR----CKCGYINRIDVDPNGRIIGDFNEIAYMLEKR 294

Query: 109 GRAGRGQNLMEAFRLALDKCNLADLVAPRNVFTWMKRVRGGTTIAERLDRFLANDEFRSL 168
           G   R ++ M  FR  L  C +++L   R  FTW K       I ERLDR + NDE+ SL
Sbjct: 295 GGLVRQESQMRKFRDMLSDCVISNLRYTRQWFTWEKGHTASNNIHERLDRAVVNDEWWSL 354

Query: 169 FRNIGISHLNKHGSDHHPIVA-SMAKSGSTK 194
           F N  +SHL+   SDH P++  S  +S STK
Sbjct: 355 FSNYKVSHLSHFFSDHCPLLLNSKVESLSTK 381

BLAST of Lag0000458 vs. ExPASy TrEMBL
Match: A0A7J8T1R6 (Uncharacterized protein OS=Gossypium davidsonii OX=34287 GN=Godav_000815 PE=4 SV=1)

HSP 1 Score: 92.8 bits (229), Expect = 1.8e-15
Identity = 59/151 (39.07%), Postives = 82/151 (54.30%), Query Frame = 0

Query: 49  IRSKNPHIVFITKTKSGSARMEKVKNDLNFDCGF-----CVSNYWGGGDFNEILFADEKR 108
           +R  NP +VF+ +TK   +R+E+++      CG+        N    GDFNEI +  EKR
Sbjct: 235 LRDSNPSVVFLIETKLQGSRLERIR----CKCGYINRIDVDPNGRIIGDFNEIAYMLEKR 294

Query: 109 GRAGRGQNLMEAFRLALDKCNLADLVAPRNVFTWMKRVRGGTTIAERLDRFLANDEFRSL 168
           G   R ++ M  FR  L  C +++L   R  FTW K       I ERLDR + NDE+ SL
Sbjct: 295 GGLVRQESQMRKFRDMLSDCVISNLRYTRQWFTWEKGHTASNNIHERLDRAVVNDEWWSL 354

Query: 169 FRNIGISHLNKHGSDHHPIVA-SMAKSGSTK 194
           F N  +SHL+   SDH P++  S  +S STK
Sbjct: 355 FSNYKVSHLSHFFSDHCPLLLNSKVESLSTK 381

BLAST of Lag0000458 vs. ExPASy TrEMBL
Match: A0A5B6WBZ4 (Reverse transcriptase OS=Gossypium australe OX=47621 GN=EPI10_019256 PE=4 SV=1)

HSP 1 Score: 90.5 bits (223), Expect = 9.2e-15
Identity = 56/145 (38.62%), Postives = 76/145 (52.41%), Query Frame = 0

Query: 47  HEIRSKNPHIVFITKTKSGSARMEKVKNDLNFDCGFCVSNYW--GG------GDFNEILF 106
           H  +   P +V   +TK    +MEKV+    F  G  V +    GG      GDFNEIL+
Sbjct: 22  HSQKLHYPQVVSFIETKINKNQMEKVRRSCGFHYGIDVDSIGSRGGLSLAWRGDFNEILY 81

Query: 107 ADEKRGRAGRGQNLMEAFRLALDKCNLADLVAPRNVFTWMKRVRGGTTIAERLDRFLAND 166
             EK+G   R +  M+AFR  L+ C+L D+    N FTW +     T I ERLDR +A +
Sbjct: 82  GFEKKGGLPREERRMKAFRKVLEDCSLVDMGFSGNWFTWERENLPETNIQERLDRGVAME 141

Query: 167 EFRSLFRNIGISHLNKHGSDHHPIV 184
           E+ SLF +  I HL    SDH P++
Sbjct: 142 EWISLFPDSLIQHLPHSFSDHCPLL 166

BLAST of Lag0000458 vs. ExPASy TrEMBL
Match: A0A7J6GL46 (CCHC-type domain-containing protein OS=Cannabis sativa OX=3483 GN=F8388_014122 PE=4 SV=1)

HSP 1 Score: 88.2 bits (217), Expect = 4.5e-14
Identity = 42/97 (43.30%), Postives = 61/97 (62.89%), Query Frame = 0

Query: 90  GGDFNEILFADEKRGRAGRGQNLMEAFRLALDKCNLADLVAPRNVFTWMKRVRGGTTIAE 149
           GGDFNEIL  +EK+G + R  + M  F+ ALD+C+LADL      FTW+ + +GG  + E
Sbjct: 601 GGDFNEILSINEKKGGSDRSMSAMTEFQNALDRCSLADLGFEGQCFTWLNKRQGGAHVQE 660

Query: 150 RLDRFLANDEFRSLFRNIGISHLNKHGSDHHPIVASM 187
           RLDR+  N  +  LF  + + + +   SDH PIVA++
Sbjct: 661 RLDRYFCNQRWHDLFPFVKVLNGDFLNSDHRPIVATL 697