Lag0000449 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0000449 vs. NCBI nr
Match: KAA0053385.1 (putative gag-pol polyprotein [Cucumis melo var. makuwa])

HSP 1 Score: 144.4 bits (363), Expect = 8.2e-31
Identity = 82/157 (52.23%), Postives = 104/157 (66.24%), Query Frame = 0

Query: 1   MKIWKKLKFVLRIQIVRNRVEFILS-------KKLFLETPQEVEDMRRMPYAIGLHGVHL 60
           MK   + ++VL IQI+R+R   +L+        K+ +    +      +P+    HGVHL
Sbjct: 186 MKDLGEAQYVLGIQIIRDRKNKMLALSQATYIDKMLVRYSMQNSKKDLLPFR---HGVHL 245

Query: 61  SKEWCPKTPQEIKDMRQIPYASAVGSLMYVMLCTRPDICYAVGIVSTYQ---------SA 120
           SKE CPKTPQE +DMR+IPYASAVG+LMYVMLCTRPDICYAVGIVS+ +           
Sbjct: 246 SKEQCPKTPQEFEDMRRIPYASAVGNLMYVMLCTRPDICYAVGIVSSIKQGCIADSTMEV 305

Query: 121 ENIASCEAAIEIVRLRKFLADLECVPNVNLPIRLYSD 142
           E +A+C+AA E + LRKFL DLE VPN+NLPI LY D
Sbjct: 306 EYVAACDAAKEAIWLRKFLHDLEVVPNMNLPITLYCD 339

BLAST of Lag0000449 vs. NCBI nr
Match: KAA0026154.1 (gag/pol protein [Cucumis melo var. makuwa] >TYK11614.1 gag/pol protein [Cucumis melo var. makuwa])

HSP 1 Score: 142.1 bits (357), Expect = 4.1e-30
Identity = 78/153 (50.98%), Postives = 99/153 (64.71%), Query Frame = 0

Query: 9   FVLRIQIVRNRVEFILSKKLFLETPQEVEDMRRMPYAIGL-----------HGVHLSKEW 68
           +V  I ++ N V ++   K +L    +++D+    Y +G+           H VHLSKE 
Sbjct: 617 YVDDILLIGNDVGYLTDVKAWLAVQFQMKDLGEAQYVLGIQNSKKSLLPFRHEVHLSKEQ 676

Query: 69  CPKTPQEIKDMRQIPYASAVGSLMYVMLCTRPDICYAVGIVSTYQ---------SAENIA 128
           CPKTPQE++DMR+IPYAS +GSLMY MLCTR DICYAVGIVS+ +          AE +A
Sbjct: 677 CPKTPQEVEDMRRIPYASVMGSLMYAMLCTRLDICYAVGIVSSIKQGCIADSTMEAEYVA 736

Query: 129 SCEAAIEIVRLRKFLADLECVPNVNLPIRLYSD 142
           +CEAA E V LRKFL DLE VPN+NLPI LY D
Sbjct: 737 ACEAAKEAVWLRKFLHDLEVVPNMNLPITLYCD 769

BLAST of Lag0000449 vs. NCBI nr
Match: TYK20422.1 (gag/pol protein [Cucumis melo var. makuwa])

HSP 1 Score: 141.4 bits (355), Expect = 7.0e-30
Identity = 85/183 (46.45%), Postives = 105/183 (57.38%), Query Frame = 0

Query: 1   MKIWKKLKFVLRIQIVRNRVEFILS-------KKLFLETPQEVEDMRRMPYAIGLHGVHL 60
           MK   + ++VLRIQI+R+R    L+        K+ +    +      +P+    HGVHL
Sbjct: 568 MKNLGEAQYVLRIQIIRDRKNKTLALSQATYIDKMLVRYSMQNSKKGLLPFR---HGVHL 627

Query: 61  SKEWCPKTPQEIKDMRQIPYASAVGSLMYVMLCTRPDICYAVGIVSTYQS---------- 120
           SKE CPKTPQE++DMR+IPYASAVGSLMY MLCTRPDICYAVGIVS +QS          
Sbjct: 628 SKEQCPKTPQEVEDMRRIPYASAVGSLMYAMLCTRPDICYAVGIVSRHQSNPGKSTSELV 687

Query: 121 -------------------------AENIASCEAAIEIVRLRKFLADLECVPNVNLPIRL 142
                                    AE + +CE A E V LRKF+ DLE +PN+NLPI L
Sbjct: 688 FTLNGGAVVWRSIKQGCIIADSTMEAEYVVACETAKEAVWLRKFIHDLEVIPNMNLPITL 747

BLAST of Lag0000449 vs. NCBI nr
Match: KAA0033228.1 (gag/pol protein [Cucumis melo var. makuwa])

HSP 1 Score: 139.8 bits (351), Expect = 2.0e-29
Identity = 82/184 (44.57%), Postives = 101/184 (54.89%), Query Frame = 0

Query: 9   FVLRIQIVRNRVEFILSKKLFLETPQEVEDMRRMPYAIG-------------LHGVHLSK 68
           +V  I ++ N V ++   K +L    +++D+    Y +G             LHGVHLSK
Sbjct: 688 YVDDILLIGNDVGYLTDVKAWLAAQFQMKDLGEAQYVLGIQIIRDRKNKTLALHGVHLSK 747

Query: 69  EWCPKTPQEIKDMRQIPYASAVGSLMYVMLCTRPDICYAVGIVSTYQS------------ 128
           E CPK P E++DMR+IPYASAVGSLM+ MLCTRPDICYAVGIVS YQS            
Sbjct: 748 EQCPKKPHEVEDMRRIPYASAVGSLMHAMLCTRPDICYAVGIVSRYQSNPGLDHWTAVKI 807

Query: 129 --------------------------AENIASCEAAIEIVRLRKFLADLECVPNVNLPIR 142
                                      E +A+CEAA E+V LRKFL DLE VPN+NLPI 
Sbjct: 808 ILKYLRRTRDYMLVIKQGCIADSTIEVEYVAACEAAKEVVWLRKFLHDLEVVPNMNLPIT 867

BLAST of Lag0000449 vs. NCBI nr
Match: KAA0060794.1 (putative Integrase core domain [Cucumis melo var. makuwa])

HSP 1 Score: 138.7 bits (348), Expect = 4.5e-29
Identity = 84/176 (47.73%), Postives = 103/176 (58.52%), Query Frame = 0

Query: 1   MKIWKKLKFVLRIQIVRNRVEFILS-------KKLFLETPQEVEDMRRMPYAIGLHGVHL 60
           MK   + ++V+ IQI+R+R    L+        K+ +    +      +P+    HGVHL
Sbjct: 597 MKDLGEAQYVIGIQIIRDRKNKTLALSQATYIDKMLVRYSMQNSKKDLLPFK---HGVHL 656

Query: 61  SKEWCPKTPQEIKDMRQIPYASAVGSLMYVMLCTRPDICYAVGIVS-------------- 120
           SKE CPKTPQE++DMR+IPYASAVGSLMY MLCTRPDICYAVGIVS              
Sbjct: 657 SKEQCPKTPQEVEDMRRIPYASAVGSLMYAMLCTRPDICYAVGIVSRKSKSGSVFTLNGG 716

Query: 121 --------------TYQSAENIASCEAAIEIVRLRKFLADLECVPNVNLPIRLYSD 142
                         +   AE +A+CEAA E V LRKFL DLE VPN+NLPI LY D
Sbjct: 717 AVVWRSIKQRCIADSTMEAEYVAACEAAKEAVWLRKFLHDLEVVPNMNLPITLYCD 769

BLAST of Lag0000449 vs. ExPASy Swiss-Prot
Match: P10978 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 77.4 bits (189), Expect = 1.6e-13
Identity = 53/137 (38.69%), Postives = 80/137 (58.39%), Query Frame = 0

Query: 10   VLRIQIVRNRV--EFILSKKLFLETPQEVEDMRR-MPYAIGLHG-VHLSKEWCPKTPQEI 69
            +L ++IVR R   +  LS++ ++E   E  +M+   P +  L G + LSK+ CP T +E 
Sbjct: 1044 ILGMKIVRERTSRKLWLSQEKYIERVLERFNMKNAKPVSTPLAGHLKLSKKMCPTTVEEK 1103

Query: 70   KDMRQIPYASAVGSLMYVMLCTRPDICYAVGIVSTYQSAENIASCEAAIEIVR-LRKFLA 129
             +M ++PY+SAVGSLMY M+CTRPDI +AVG+VS +         EA   I+R LR    
Sbjct: 1104 GNMAKVPYSSAVGSLMYAMVCTRPDIAHAVGVVSRFLENPGKEHWEAVKWILRYLRGTTG 1163

Query: 130  DLECVPNVNLPIRLYSD 142
            D  C    +  ++ Y+D
Sbjct: 1164 DCLCFGGSDPILKGYTD 1180

BLAST of Lag0000449 vs. ExPASy Swiss-Prot
Match: P04146 (Copia protein OS=Drosophila melanogaster OX=7227 GN=GIP PE=1 SV=3)

HSP 1 Score: 48.1 bits (113), Expect = 1.1e-04
Identity = 25/63 (39.68%), Postives = 35/63 (55.56%), Query Frame = 0

Query: 72   PYASAVGSLMYVMLCTRPDICYAVGIVSTYQSAENIASCEAAIEIVRLRKFLADLECVPN 131
            P  S +G LMY+MLCTRPD+  AV I+S Y S  N    +    ++R  K   D++ +  
Sbjct: 1180 PCRSLIGCLMYIMLCTRPDLTTAVNILSRYSSKNNSELWQNLKRVLRYLKGTIDMKLIFK 1239

Query: 132  VNL 135
             NL
Sbjct: 1240 KNL 1242

BLAST of Lag0000449 vs. ExPASy Swiss-Prot
Match: P0CV72 (Secreted RxLR effector protein 161 OS=Plasmopara viticola OX=143451 GN=RXLR161 PE=2 SV=1)

HSP 1 Score: 47.0 bits (110), Expect = 2.3e-04
Identity = 19/36 (52.78%), Postives = 29/36 (80.56%), Query Frame = 0

Query: 68  MRQIPYASAVGSLMYVMLCTRPDICYAVGIVSTYQS 104
           M+ +PY SAVG++MY+M+ TRPD+  AVG++S + S
Sbjct: 1   MKNVPYLSAVGAIMYLMVVTRPDLAAAVGVLSQFAS 36