Lag0000342 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0000342
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionLINE-1 retrotransposable element ORF2 protein
Locationchr4: 4675662 .. 4677640 (-)
RNA-Seq ExpressionLag0000342
SyntenyLag0000342
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCAAGAGATCAGGATGTGGAAGCAAAGATGCAAGAAAACCTGGCTTAAGGAAGGAGATGAAAATACCACCTTTTTCCATAAAGTATGTACTGCTCGTCGGCGACGATGCTTCATTTCAGAGATGATTTCGGGAAATGGGATAAGCCTGATTACAGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTCTCAAATGGAGTTGGAGGTGATTAATCATTTCTCTAACATTTATAGGGCACCACTTCTGAAGGATGGATGGTCTCAAATTTAAATTGGTGCCCTATTTCTGGTCCTGAGGCGACATCTTTGATCCAGCCTTTTTCAGAGCTAGAAGTATTTCAGAATCTAAAAGCAATGGGTCATAACAAGTCCCCTGGCCCGGATGGATTCACAGTTGAATTCTTTAAAAAGTCCTGGATTGCTATCAGGCCTTCAGTTATGGAAGTGTTCCGTGACTTCTTTCAGAAGGGTATCATCAACTGTAATGTTAATGAAACCTACATTACTTTGATACCAAAGAAGAATAAAGCTATGCAAATTCAAGACTTTAGACCCATTAGCCTCACCACAGTTCTCTATCGCCTTATCGCTAAGACTCTTGCAGAAAGGCTTAAAAGTACTCTTCAAGGGACAATATCTGAGAATCAACTGGCATTTGTAAAAGGTCGTCAAATTACTGATGCTATTTTGGTGGCTAATGAAGCTATTGATTTTTGGAAATGCTCTCGCACGAGAGGATTTGTTATAAAGCTAGATATTGAAAAGGCTTTTGACAAGATCTGTTGGAACTTCGTTGATAAGATTCTTGCTTTCAAGGGATACCCTATCACCTGGCGTAAGTGGATCCGGGCTTGCATCACCTCAGTTTCATACTCCATATTGCTTAACGGGAAGCCAAGAGGAAAATCAAGGCCGAAAGAGGCATTCGTCAAGGAGATCCGATCTCCCCTTTTATCTTTGTCCTAGCTATGGACTATCTTAGTCGAATTCTTCAGTCGGCTGAGCAAAAGGGGCTTGTTAAGGGTTGTTCTCTCAACTCCGTCTCTGTCTCTCATCTTCTATTCGCAGATGACATTCTCCTTTTTGTTCAAGATAACGATGCTATGTTAGGCAACCTGTTCAACATCATCAAAGTATTTGAGCTCTCTTCGGGTCTCAATATAAACTTCAGCAAATCCTCTATAACGGGTATCAACGTGGAGGATTCCAGAGTTGCTCAAATTGCCGCCAATTGGGGATGCCCAACGACCCAATTTCCCATTCCTTATTTAGGCTCTCCCTTGGGGGGTAATCCATCATCGTCTTCGTTCTGGGCTAATACGGTTGATAAGATTCATCGTAAATTGGATAGTTGGCGCTATTCCTATATTTCTAAAGGAGGAAGACTAACCTTGATAAGAGCAACTTTAAGTGGCATTCCCAACTACTTGTTATCTATCTTTAAAGCTCCGCAATCGGTTTGCTTTAGTATAGATAAGATTATCAGATCTTTCCTTTGGCATGGGCATGACCAAAGTAGTAGCATCCCTTTGGTTAGCTGGGATAAGGTGGCTGCGCCTATTGAGGCGGGGGTTTGGGCTTATTCAAGACTAGAATCACAAATAGCGCATTTCAAGTCAAATGGCTTTGGAGATTCTTTCATGAGGAGACTTCTCTGTGGAAGCGAGTCATTTCAGCAAAATATACAACCCAAAGACAGGGAGCTCTCCCAACTCAGACGCGATACACTTCCTCCCGAGCGCCATGGACATCAATTCTCAAGCAAGCATCATCTTTTCTGGCAAATACAGCTTGGAATCTTAAGGATGGCAGTAAAATCTCTTTTTGGCATGACTCTTGGACCGATCATGGGCCATTACATCAAGCCCTCCCTCGGCTCTTTGCGCTGTCCAGTAGGAAAGATATGA

mRNA sequence

ATGCAAGAGATCAGGATGTGGAAGCAAAGATGCAAGAAAACCTGGCTTAAGGAAGGAGATGAAAATACCACCTTTTTCCATAAAGGCACCACTTCTGAAGGATGGATGGTCTCAAATTTAAATTGGTGCCCTATTTCTGGTCCTGAGGCGACATCTTTGATCCAGCCTTTTTCAGAGCTAGAAGTATTTCAGAATCTAAAAGCAATGGGTCATAACAAGTCCCCTGGCCCGGATGGATTCACAGTTGAATTCTTTAAAAAGTCCTGGATTGCTATCAGGCCTTCAGTTATGGAAGTGTTCCGTGACTTCTTTCAGAAGGGTATCATCAACTGTAATGTTAATGAAACCTACATTACTTTGATACCAAAGAAGAATAAAGCTATGCAAATTCAAGACTTTAGACCCATTAGCCTCACCACAGTTCTCTATCGCCTTATCGCTAAGACTCTTGCAGAAAGGCTTAAAAGTACTCTTCAAGGGACAATATCTGAGAATCAACTGGCATTTGTAAAAGGTCGTCAAATTACTGATGCTATTTTGGTGGCTAATGAAGCTATTGATTTTTGGAAATGCTCTCGCACGAGAGGATTTGTTATAAAGCTAGATATTGAAAAGGCTTTTGACAAGATCTGTTGGAACTTCGTTGATAAGATTCTTGCTTTCAAGGGATACCCTATCACCTGGCCCAAGAGGAAAATCAAGGCCGAAAGAGGCATTCGTCAAGGAGATCCGATCTCCCCTTTTATCTTTGTCCTAGCTATGGACTATCTTAGTCGAATTCTTCAGTCGGCTGAGCAAAAGGGGCTTGTTAAGGGTTGTTCTCTCAACTCCGTCTCTGTCTCTCATCTTCTATTCGCAGATGACATTCTCCTTTTTGTTCAAGATAACGATGCTATGTTAGGCAACCTGTTCAACATCATCAAAGTATTTGAGCTCTCTTCGGGTCTCAATATAAACTTCAGCAAATCCTCTATAACGGGTATCAACGTGGAGGATTCCAGAGTTGCTCAAATTGCCGCCAATTGGGGATGCCCAACGACCCAATTTCCCATTCCTTATTTAGGCTCTCCCTTGGGGGGTAATCCATCATCGTCTTCGTTCTGGGCTAATACGGTTGATAAGATTCATCGTAAATTGGATAGTTGGCGCTATTCCTATATTTCTAAAGGAGGAAGACTAACCTTGATAAGAGCAACTTTAAGTGGCATTCCCAACTACTTGTTATCTATCTTTAAAGCTCCGCAATCGGTTTGCTTTAGTATAGATAAGATTATCAGATCTTTCCTTTGGCATGGGCATGACCAAAGTAGTAGCATCCCTTTGGTTAGCTGGGATAAGGTGGCTGCGCCTATTGAGGCGGGGGTTTGGGCTTATTCAAGACTAGAATCACAAATAGCGCATTTCAAGTCAAATGGCTTTGGAGATTCTTTCATGAGGAGACTTCTCTGTGGAAGCGAGTCATTTCAGCAAAATATACAACCCAAAGACAGGGAGCTCTCCCAACTCAGACGCGATACACTTCCTCCCGAGCGCCATGGACATCAATTCTCAAGCAAGCATCATCTTTTCTGGCAAATACAGCTTGGAATCTTAAGGATGGCAGTAAAATCTCTTTTTGGCATGACTCTTGGACCGATCATGGGCCATTACATCAAGCCCTCCCTCGGCTCTTTGCGCTGTCCAGTAGGAAAGATATGA

Coding sequence (CDS)

ATGCAAGAGATCAGGATGTGGAAGCAAAGATGCAAGAAAACCTGGCTTAAGGAAGGAGATGAAAATACCACCTTTTTCCATAAAGGCACCACTTCTGAAGGATGGATGGTCTCAAATTTAAATTGGTGCCCTATTTCTGGTCCTGAGGCGACATCTTTGATCCAGCCTTTTTCAGAGCTAGAAGTATTTCAGAATCTAAAAGCAATGGGTCATAACAAGTCCCCTGGCCCGGATGGATTCACAGTTGAATTCTTTAAAAAGTCCTGGATTGCTATCAGGCCTTCAGTTATGGAAGTGTTCCGTGACTTCTTTCAGAAGGGTATCATCAACTGTAATGTTAATGAAACCTACATTACTTTGATACCAAAGAAGAATAAAGCTATGCAAATTCAAGACTTTAGACCCATTAGCCTCACCACAGTTCTCTATCGCCTTATCGCTAAGACTCTTGCAGAAAGGCTTAAAAGTACTCTTCAAGGGACAATATCTGAGAATCAACTGGCATTTGTAAAAGGTCGTCAAATTACTGATGCTATTTTGGTGGCTAATGAAGCTATTGATTTTTGGAAATGCTCTCGCACGAGAGGATTTGTTATAAAGCTAGATATTGAAAAGGCTTTTGACAAGATCTGTTGGAACTTCGTTGATAAGATTCTTGCTTTCAAGGGATACCCTATCACCTGGCCCAAGAGGAAAATCAAGGCCGAAAGAGGCATTCGTCAAGGAGATCCGATCTCCCCTTTTATCTTTGTCCTAGCTATGGACTATCTTAGTCGAATTCTTCAGTCGGCTGAGCAAAAGGGGCTTGTTAAGGGTTGTTCTCTCAACTCCGTCTCTGTCTCTCATCTTCTATTCGCAGATGACATTCTCCTTTTTGTTCAAGATAACGATGCTATGTTAGGCAACCTGTTCAACATCATCAAAGTATTTGAGCTCTCTTCGGGTCTCAATATAAACTTCAGCAAATCCTCTATAACGGGTATCAACGTGGAGGATTCCAGAGTTGCTCAAATTGCCGCCAATTGGGGATGCCCAACGACCCAATTTCCCATTCCTTATTTAGGCTCTCCCTTGGGGGGTAATCCATCATCGTCTTCGTTCTGGGCTAATACGGTTGATAAGATTCATCGTAAATTGGATAGTTGGCGCTATTCCTATATTTCTAAAGGAGGAAGACTAACCTTGATAAGAGCAACTTTAAGTGGCATTCCCAACTACTTGTTATCTATCTTTAAAGCTCCGCAATCGGTTTGCTTTAGTATAGATAAGATTATCAGATCTTTCCTTTGGCATGGGCATGACCAAAGTAGTAGCATCCCTTTGGTTAGCTGGGATAAGGTGGCTGCGCCTATTGAGGCGGGGGTTTGGGCTTATTCAAGACTAGAATCACAAATAGCGCATTTCAAGTCAAATGGCTTTGGAGATTCTTTCATGAGGAGACTTCTCTGTGGAAGCGAGTCATTTCAGCAAAATATACAACCCAAAGACAGGGAGCTCTCCCAACTCAGACGCGATACACTTCCTCCCGAGCGCCATGGACATCAATTCTCAAGCAAGCATCATCTTTTCTGGCAAATACAGCTTGGAATCTTAAGGATGGCAGTAAAATCTCTTTTTGGCATGACTCTTGGACCGATCATGGGCCATTACATCAAGCCCTCCCTCGGCTCTTTGCGCTGTCCAGTAGGAAAGATATGA

Protein sequence

MQEIRMWKQRCKKTWLKEGDENTTFFHKGTTSEGWMVSNLNWCPISGPEATSLIQPFSELEVFQNLKAMGHNKSPGPDGFTVEFFKKSWIAIRPSVMEVFRDFFQKGIINCNVNETYITLIPKKNKAMQIQDFRPISLTTVLYRLIAKTLAERLKSTLQGTISENQLAFVKGRQITDAILVANEAIDFWKCSRTRGFVIKLDIEKAFDKICWNFVDKILAFKGYPITWPKRKIKAERGIRQGDPISPFIFVLAMDYLSRILQSAEQKGLVKGCSLNSVSVSHLLFADDILLFVQDNDAMLGNLFNIIKVFELSSGLNINFSKSSITGINVEDSRVAQIAANWGCPTTQFPIPYLGSPLGGNPSSSSFWANTVDKIHRKLDSWRYSYISKGGRLTLIRATLSGIPNYLLSIFKAPQSVCFSIDKIIRSFLWHGHDQSSSIPLVSWDKVAAPIEAGVWAYSRLESQIAHFKSNGFGDSFMRRLLCGSESFQQNIQPKDRELSQLRRDTLPPERHGHQFSSKHHLFWQIQLGILRMAVKSLFGMTLGPIMGHYIKPSLGSLRCPVGKI
Homology
BLAST of Lag0000342 vs. NCBI nr
Match: KAA0039770.1 (LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa] >TYK24727.1 LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa])

HSP 1 Score: 493.4 bits (1269), Expect = 2.6e-135
Identity = 252/524 (48.09%), Postives = 338/524 (64.50%), Query Frame = 0

Query: 2   QEIRMWKQRCKKTWLKEGDENTTFFHK--------------------------------- 61
           +E ++W Q+ K+ W+ EGDENT+FFHK                                 
Sbjct: 181 KEAQIWYQKSKRLWITEGDENTSFFHKICSARQRRSIISNINSVDGVPCSTNESIAKAFL 240

Query: 62  ---------GTTSEGWMVSNLNWCPISGPEATSLIQPFSELEVFQNLKAMGHNKSPGPDG 121
                    G     W++ NLNW PIS  +A +L   F+E E+ + L A  +NKSPGPDG
Sbjct: 241 DHFEDIYKGGGEESPWLIDNLNWSPISTNQAQNLCSMFTEEEIHEALTAFSNNKSPGPDG 300

Query: 122 FTVEFFKKSWIAIRPSVMEVFRDFFQKGIINCNVNETYITLIPKKNKAMQIQDFRPISLT 181
           FT+EF+K +W  ++  ++ +FRDF    IIN  VN T I LI KK K  +  D+RPISLT
Sbjct: 301 FTMEFYKSTWSVLKEEILNIFRDFHSNCIINKAVNITNIALIAKKEKCAEPADYRPISLT 360

Query: 182 TVLYRLIAKTLAERLKSTLQGTISENQLAFVKGRQITDAILVANEAIDFWKCSRTRGFVI 241
           T +Y+LIAK +AERLK TL  T++ENQ+AFVKGRQI DAILVANEAID+W+  + +GFVI
Sbjct: 361 TSIYKLIAKVIAERLKDTLPYTVAENQMAFVKGRQIIDAILVANEAIDYWRVKKIQGFVI 420

Query: 242 KLDIEKAFDKICWNFVDKILAFKGYPITW--------------------PKRKIKAERGI 301
           KLDIEKAFDK+ W F+D +L  KGYP  W                    P+ KI+  RGI
Sbjct: 421 KLDIEKAFDKLNWRFIDFMLMKKGYPFKWRNWIRACISSVQYSIIINGRPRGKIQPSRGI 480

Query: 302 RQGDPISPFIFVLAMDYLSRILQSAEQKGLVKGCSL-NSVSVSHLLFADDILLFVQDNDA 361
           RQGDPISPFIFVLAMDY+SR+L S  +K  +KG  L  +++++HLLFADDILLFV+D++ 
Sbjct: 481 RQGDPISPFIFVLAMDYMSRLLNSVGEK--IKGVKLEGNINLTHLLFADDILLFVEDDEH 540

Query: 362 MLGNLFNIIKVFELSSGLNINFSKSSITGINVEDSRVAQIAANWGCPTTQFPIPYLGSPL 421
            + NL NII +F+L+SGL+IN +KS+I+ INV+ SR  QIA+ WG  T   PI YLG PL
Sbjct: 541 SIQNLKNIINLFQLASGLSINLNKSTISPINVDASRTEQIASQWGISTKFLPINYLGVPL 600

Query: 422 GGNPSSSSFWANTVDKIHRKLDSWRYSYISKGGRLTLIRATLSGIPNYLLSIFKAPQSVC 463
           GG   + +FW N  +KI++KL SW+YS +SKGG++TLI+++L+ +P Y LSIFKAP S C
Sbjct: 601 GGKQITKTFWKNVEEKINKKLASWKYSMLSKGGKITLIKSSLASLPTYQLSIFKAPVSTC 660

BLAST of Lag0000342 vs. NCBI nr
Match: KAA0041367.1 (LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa])

HSP 1 Score: 493.0 bits (1268), Expect = 3.3e-135
Identity = 250/524 (47.71%), Postives = 339/524 (64.69%), Query Frame = 0

Query: 2   QEIRMWKQRCKKTWLKEGDENTTFFHK--------------------------------- 61
           +E ++W Q+ K+ W+ EGDENT+FFHK                                 
Sbjct: 5   KEAQIWYQKSKRLWITEGDENTSFFHKICTARQRRSIISNIKSVDGVPCSTNESIAKAFL 64

Query: 62  ---------GTTSEGWMVSNLNWCPISGPEATSLIQPFSELEVFQNLKAMGHNKSPGPDG 121
                    G     W++ NL+W PIS  +A +L   F+E E+   L A  +NKSPGPDG
Sbjct: 65  DHFEDIYKGGGEESPWLIDNLSWSPISTTQAQNLCSCFTEEEIHAALTAFSNNKSPGPDG 124

Query: 122 FTVEFFKKSWIAIRPSVMEVFRDFFQKGIINCNVNETYITLIPKKNKAMQIQDFRPISLT 181
           FT+EF+K +W  ++  ++ +FRDF    IIN  VN T I LI KK K  +  D+RPISLT
Sbjct: 125 FTMEFYKSTWSDLKEEILNIFRDFHSNCIINKAVNITNIALIAKKEKCAEPADYRPISLT 184

Query: 182 TVLYRLIAKTLAERLKSTLQGTISENQLAFVKGRQITDAILVANEAIDFWKCSRTRGFVI 241
           T +Y+LIAK +AERLK TL  T++ENQ+AFVKGRQI DAILVANEAID+W+  + +GFVI
Sbjct: 185 TSIYKLIAKVIAERLKETLPSTVAENQMAFVKGRQIIDAILVANEAIDYWRVKKIQGFVI 244

Query: 242 KLDIEKAFDKICWNFVDKILAFKGYPITW--------------------PKRKIKAERGI 301
           KLDIEKAFDK+ W F+D +L  KGYP  W                    P+ KI+  RGI
Sbjct: 245 KLDIEKAFDKLNWRFIDFMLMKKGYPFRWRRWIRACISSVQYSIIINGRPRGKIQPSRGI 304

Query: 302 RQGDPISPFIFVLAMDYLSRILQSAEQKGLVKGCSL-NSVSVSHLLFADDILLFVQDNDA 361
           RQGDPISPFIFVLAMDY+SR+L S  +K  +KG  +  +++++HLLFADDILLFV+D++ 
Sbjct: 305 RQGDPISPFIFVLAMDYISRLLNSVGEK--IKGVKMEGNINLTHLLFADDILLFVEDDEH 364

Query: 362 MLGNLFNIIKVFELSSGLNINFSKSSITGINVEDSRVAQIAANWGCPTTQFPIPYLGSPL 421
            + NL NII +F+L+SGL+IN +KS+I+ INV+ +R  QIA+ WG  T   PI YLG PL
Sbjct: 365 SIQNLKNIINLFQLASGLSINLNKSTISPINVDAARTEQIASQWGISTKFLPINYLGVPL 424

Query: 422 GGNPSSSSFWANTVDKIHRKLDSWRYSYISKGGRLTLIRATLSGIPNYLLSIFKAPQSVC 463
           GG  ++ +FW N  +KI++KL SW+YS +SKGG++TLI+++L+ +P Y LSIFKAP S C
Sbjct: 425 GGKQTTKTFWKNVEEKINKKLASWKYSMLSKGGKITLIKSSLASLPTYQLSIFKAPVSAC 484

BLAST of Lag0000342 vs. NCBI nr
Match: XP_016902461.1 (PREDICTED: LINE-1 retrotransposable element ORF2 protein [Cucumis melo])

HSP 1 Score: 491.9 bits (1265), Expect = 7.4e-135
Identity = 251/524 (47.90%), Postives = 337/524 (64.31%), Query Frame = 0

Query: 2   QEIRMWKQRCKKTWLKEGDENTTFFHK--------------------------------- 61
           +E ++W Q+ K+ W+ EGDENT+FFHK                                 
Sbjct: 181 KEAQIWYQKSKRLWITEGDENTSFFHKICSARQRRSIISNINSVDGVPCSTNESIAKAFL 240

Query: 62  ---------GTTSEGWMVSNLNWCPISGPEATSLIQPFSELEVFQNLKAMGHNKSPGPDG 121
                    G     W++ NLNW PIS  +A +L   F+E E+ + L A  +NKSPGPDG
Sbjct: 241 DHFEDIYKGGGEESPWLIDNLNWSPISTNQAQNLCSMFTEEEIHEALTAFSNNKSPGPDG 300

Query: 122 FTVEFFKKSWIAIRPSVMEVFRDFFQKGIINCNVNETYITLIPKKNKAMQIQDFRPISLT 181
           FT+EF+K +W  ++  ++ +FRDF    IIN  VN T I LI KK K  +  D+RPISLT
Sbjct: 301 FTMEFYKSTWSVLKEEILNIFRDFHSNCIINKAVNITNIALIAKKEKCAEPADYRPISLT 360

Query: 182 TVLYRLIAKTLAERLKSTLQGTISENQLAFVKGRQITDAILVANEAIDFWKCSRTRGFVI 241
           T +Y+LIAK +AERLK TL  T++ENQ+AFVKGRQI DAILVANEAID+W+  + +GFVI
Sbjct: 361 TSIYKLIAKVIAERLKDTLPYTVAENQMAFVKGRQIIDAILVANEAIDYWRVKKIQGFVI 420

Query: 242 KLDIEKAFDKICWNFVDKILAFKGYPITW--------------------PKRKIKAERGI 301
           KLDIEKAFDK+ W F+D +L  KGYP  W                    P+ KI+  RGI
Sbjct: 421 KLDIEKAFDKLNWRFIDFMLMKKGYPFKWRNWIRACISSVQYSIIINGRPRGKIQPSRGI 480

Query: 302 RQGDPISPFIFVLAMDYLSRILQSAEQKGLVKGCSL-NSVSVSHLLFADDILLFVQDNDA 361
           RQGDPISPFIFVLAMDY+SR+L S  +K  +KG  L  +++++HLLFADDILLFV+D++ 
Sbjct: 481 RQGDPISPFIFVLAMDYMSRLLNSVGEK--IKGVKLEGNINLTHLLFADDILLFVEDDEH 540

Query: 362 MLGNLFNIIKVFELSSGLNINFSKSSITGINVEDSRVAQIAANWGCPTTQFPIPYLGSPL 421
            + NL NII +F+L+SGL+IN +KS+I+ INV+ SR  QIA+ WG  T   PI YLG PL
Sbjct: 541 SIQNLKNIINLFQLASGLSINLNKSTISPINVDASRTEQIASQWGISTKFLPINYLGVPL 600

Query: 422 GGNPSSSSFWANTVDKIHRKLDSWRYSYISKGGRLTLIRATLSGIPNYLLSIFKAPQSVC 463
           GG   + +FW N  +KI++KL SW+YS +SKGG++TLI+++L+ +P Y LSIFK P S C
Sbjct: 601 GGKQITKTFWKNVEEKINKKLASWKYSMLSKGGKITLIKSSLASLPTYQLSIFKVPVSTC 660

BLAST of Lag0000342 vs. NCBI nr
Match: KAA0046762.1 (LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa])

HSP 1 Score: 489.6 bits (1259), Expect = 3.7e-134
Identity = 241/524 (45.99%), Postives = 330/524 (62.98%), Query Frame = 0

Query: 1   MQEIRMWKQRCKKTWLKEGDENTTFFHK-------------------------------- 60
           ++E + W QR KK WL+EGDEN++FFH+                                
Sbjct: 180 LKESQFWYQRAKKLWLREGDENSSFFHRICTARQKRNFIQEIQDEEGLIQNSNDSISSAF 239

Query: 61  ----------GTTSEGWMVSNLNWCPISGPEATSLIQPFSELEVFQNLKAMGHNKSPGPD 120
                      T S+ + + NL W PI   E  +L  PF E E+   + ++   K+PGPD
Sbjct: 240 IKFFSRIFRGPTKSDPFFIDNLEWNPIEHSEGLNLCAPFLEDEIKGVINSLDGKKTPGPD 299

Query: 121 GFTVEFFKKSWIAIRPSVMEVFRDFFQKGIINCNVNETYITLIPKKNKAMQIQDFRPISL 180
           GF + FFK  W  ++  +M++F+DF+ KG+IN N+N TYI LIPKK      +DFRPISL
Sbjct: 300 GFPISFFKSYWYLLKEDIMDIFKDFYDKGVINKNMNNTYIALIPKKKDFSNPKDFRPISL 359

Query: 181 TTVLYRLIAKTLAERLKSTLQGTISENQLAFVKGRQITDAILVANEAIDFWKCSRTRGFV 240
           TT +Y++IAKTL+ RLK++L  TISENQLAFVK RQITDAIL+ANEA+DFWK  + +GF+
Sbjct: 360 TTSIYKIIAKTLSNRLKTSLPNTISENQLAFVKNRQITDAILMANEAVDFWKVKKIKGFI 419

Query: 241 IKLDIEKAFDKICWNFVDKILAFKGYPITW--------------------PKRKIKAERG 300
           +KLDIEKAFD + W+F+D +L  K +PI W                    P+ +IKA RG
Sbjct: 420 LKLDIEKAFDNLNWDFIDFVLEKKNFPILWRKWIRGCISNVTYSIIVNGRPQGRIKANRG 479

Query: 301 IRQGDPISPFIFVLAMDYLSRILQSAEQKGLVKGCSLNS-VSVSHLLFADDILLFVQDND 360
           +RQGDP+SPF+FV+AMDYLSR+L   E  G +KG S +S  ++SH+LFADDILLF++DND
Sbjct: 480 LRQGDPLSPFLFVIAMDYLSRLLSHLEITGAIKGVSFSSNCNISHILFADDILLFIEDND 539

Query: 361 AMLGNLFNIIKVFELSSGLNINFSKSSITGINVEDSRVAQIAANWGCPTTQFPIPYLGSP 420
             L NL   + +FE +SGL IN  KS++  +NV + R  + A+ WG  +   P+ YLG P
Sbjct: 540 FFLNNLRMALSLFERASGLKINLLKSALVPMNVSEIRAKECASFWGISSQSLPLSYLGVP 599

Query: 421 LGGNPSSSSFWANTVDKIHRKLDSWRYSYISKGGRLTLIRATLSGIPNYLLSIFKAPQSV 462
           LGGNP S  FW+N  +KI +KL++W+Y+ ISKGGRLTLI++TLS +P Y LS+F+AP   
Sbjct: 600 LGGNPKSRLFWSNVEEKIQKKLNNWKYAQISKGGRLTLIKSTLSSLPTYQLSVFQAPSLT 659

BLAST of Lag0000342 vs. NCBI nr
Match: KAA0037445.1 (LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa] >TYK01919.1 LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa])

HSP 1 Score: 483.8 bits (1244), Expect = 2.0e-132
Identity = 243/524 (46.37%), Postives = 326/524 (62.21%), Query Frame = 0

Query: 1   MQEIRMWKQRCKKTWLKEGDENTTFFHK-------------------------------- 60
           ++E + W QR KK WL+EGDEN++FFH+                                
Sbjct: 180 LKESQFWYQRAKKLWLREGDENSSFFHRICSSRQKRSFIHEIQDEEGLIQNTNNGISTAF 239

Query: 61  ----------GTTSEGWMVSNLNWCPISGPEATSLIQPFSELEVFQNLKAMGHNKSPGPD 120
                      T S+   + NL+W PI   E   L  PF E E+   + ++   K+PGPD
Sbjct: 240 IKFFSRIYRSSTKSDPLFIDNLDWNPIEHSEWPHLCAPFLEGEIKGVINSLDGKKTPGPD 299

Query: 121 GFTVEFFKKSWIAIRPSVMEVFRDFFQKGIINCNVNETYITLIPKKNKAMQIQDFRPISL 180
           GF + FFK  W  ++  +M++F+DF+ KG+IN N+N TYI LIPKK      +DFRPISL
Sbjct: 300 GFPISFFKSYWYLLKEDIMDIFKDFYDKGVINKNMNNTYIALIPKKKNYSHPKDFRPISL 359

Query: 181 TTVLYRLIAKTLAERLKSTLQGTISENQLAFVKGRQITDAILVANEAIDFWKCSRTRGFV 240
           TT +Y++IAKTL+ RLK+ L  TIS NQLAFVK RQITDAIL+ANEA+DFWK  + +GF+
Sbjct: 360 TTSIYKIIAKTLSNRLKTALPDTISGNQLAFVKNRQITDAILMANEALDFWKVKKIKGFI 419

Query: 241 IKLDIEKAFDKICWNFVDKILAFKGYPITW--------------------PKRKIKAERG 300
           +KLDIEKAFD + W+F+D +L  K +P  W                    P+ +IKA RG
Sbjct: 420 LKLDIEKAFDNLNWDFIDFVLEKKNFPNLWRKWIRGCISNVTYSVIVNGRPQGRIKANRG 479

Query: 301 IRQGDPISPFIFVLAMDYLSRILQSAEQKGLVKGCSLNS-VSVSHLLFADDILLFVQDND 360
           +RQGDP+SPF+FV+AMDYLSR+L   E  G +KG SLNS  ++SH+LFADDILLF++DND
Sbjct: 480 LRQGDPLSPFLFVIAMDYLSRLLSHLESSGAIKGVSLNSNCNISHILFADDILLFIEDND 539

Query: 361 AMLGNLFNIIKVFELSSGLNINFSKSSITGINVEDSRVAQIAANWGCPTTQFPIPYLGSP 420
             L NL   + +FE +SGL IN  KS++  +NV  +R  + A+ WG      P+ YLG P
Sbjct: 540 YFLKNLRMALSLFEKASGLKINLLKSALVPVNVSVNRAKECASFWGISCHSLPLSYLGVP 599

Query: 421 LGGNPSSSSFWANTVDKIHRKLDSWRYSYISKGGRLTLIRATLSGIPNYLLSIFKAPQSV 462
           LGGNP S+ FW N  DKI +KL++W+Y+ ISKGGRLTLI++TLS +P Y LS+F+ P   
Sbjct: 600 LGGNPKSNLFWRNVEDKIQKKLNNWKYAQISKGGRLTLIKSTLSSLPIYQLSVFQDPSLT 659

BLAST of Lag0000342 vs. ExPASy Swiss-Prot
Match: O00370 (LINE-1 retrotransposable element ORF2 protein OS=Homo sapiens OX=9606 PE=1 SV=1)

HSP 1 Score: 141.7 bits (356), Expect = 2.5e-32
Identity = 108/409 (26.41%), Postives = 190/409 (46.45%), Query Frame = 0

Query: 49  EATSLIQPFSELEVFQNLKAMGHNKSPGPDGFTVEFFKKSWIAIRPSVMEVFRDFFQKGI 108
           E  SL +P +  E+   + ++   KSPGPDGFT EF+++    + P ++++F+   ++GI
Sbjct: 445 EVESLNRPITGSEIVAIINSLPTKKSPGPDGFTAEFYQRYKEELVPFLLKLFQSIEKEGI 504

Query: 109 INCNVNETYITLIPKKNK-AMQIQDFRPISLTTVLYRLIAKTLAERLKSTLQGTISENQL 168
           +  +  E  I LIPK  +   + ++FRPISL  +  +++ K LA R++  ++  I  +Q+
Sbjct: 505 LPNSFYEASIILIPKPGRDTTKKENFRPISLMNIDAKILNKILANRIQQHIKKLIHHDQV 564

Query: 169 AFVKGRQITDAILVANEAIDFWKCSRTRGFV-IKLDIEKAFDKICWNFVDKILAFKGYPI 228
            F+ G Q    I  +   I     ++ +  V I +D EKAFDKI   F+ K L   G   
Sbjct: 565 GFIPGMQGWFNIRKSINVIQHINRAKDKNHVIISIDAEKAFDKIQQPFMLKTLNKLGIDG 624

Query: 229 TWPK-----------------RKIKA---ERGIRQGDPISPFIFVLAMDYLSRILQSAEQ 288
            + K                 +K++A   + G RQG P+SP +F + ++ L+R ++  ++
Sbjct: 625 MYLKIIRAIYDKPTANIILNGQKLEAFPLKTGTRQGCPLSPLLFNIVLEVLARAIRQEKE 684

Query: 289 KGLVKGCSLNSVSVSHLLFADDILLFVQDNDAMLGNLFNIIKVFELSSGLNINFSKSSIT 348
              +KG  L    V   LFADD+++++++      NL  +I  F   SG  IN  KS   
Sbjct: 685 ---IKGIQLGKEEVKLSLFADDMIVYLENPIVSAQNLLKLISNFSKVSGYKINVQKSQAF 744

Query: 349 GINVEDSRVAQIAANWGCPTTQFPIPYLGSPLGGNPSS--SSFWANTVDKIHRKLDSWRY 408
             N      +QI            I YLG  L  +        +   + +I    + W+ 
Sbjct: 745 LYNNNRQTESQIMGELPFTIASKRIKYLGIQLTRDVKDLFKENYKPLLKEIKEDTNKWKN 804

Query: 409 SYISKGGRLTLIRATLSGIPNYLLSI--FKAPQSVCFSIDKIIRSFLWH 432
              S  GR+ +++  +     Y  +    K P +    ++K    F+W+
Sbjct: 805 IPCSWVGRINIVKMAILPKVIYRFNAIPIKLPMTFFTELEKTTLKFIWN 850

BLAST of Lag0000342 vs. ExPASy Swiss-Prot
Match: P08548 (LINE-1 reverse transcriptase homolog OS=Nycticebus coucang OX=9470 PE=4 SV=1)

HSP 1 Score: 139.4 bits (350), Expect = 1.2e-31
Identity = 110/415 (26.51%), Postives = 191/415 (46.02%), Query Frame = 0

Query: 45  ISGPEATSLIQPFSELEVFQNLKAMGHNKSPGPDGFTVEFFKKSWIAIRPSVMEVFRDFF 104
           +S  E   L +P S  E+   ++ +   KSPGPDGFT EF++     + P ++ +F++  
Sbjct: 440 LSQKEVEMLNRPISSSEIASTIQNLPKKKSPGPDGFTSEFYQTFKEELVPILLNLFQNIE 499

Query: 105 QKGIINCNVNETYITLIPKKNK-AMQIQDFRPISLTTVLYRLIAKTLAERLKSTLQGTIS 164
           ++GI+     E  ITLIPK  K   + +++RPISL  +  +++ K L  R++  ++  I 
Sbjct: 500 KEGILPNTFYEANITLIPKPGKDPTRKENYRPISLMNIDAKILNKILTNRIQQHIKKIIH 559

Query: 165 ENQLAFVKGRQITDAILVANEAID-FWKCSRTRGFVIKLDIEKAFDKICWNFVDKILAFK 224
            +Q+ F+ G Q    I  +   I    K       ++ +D EKAFD I   F+ + L   
Sbjct: 560 HDQVGFIPGSQGWFNIRKSINVIQHINKLKNKDHMILSIDAEKAFDNIQHPFMIRTLKKI 619

Query: 225 GYPITWPK--------------------RKIKAERGIRQGDPISPFIFVLAMDYLSRILQ 284
           G   T+ K                    +      G RQG P+SP +F + M+ L+  ++
Sbjct: 620 GIEGTFLKLIEAIYSKPTANIILNGVKLKSFPLRSGTRQGCPLSPLLFNIVMEVLAIAIR 679

Query: 285 SAEQKGLVKGCSLNSVSVSHLLFADDILLFVQDNDAMLGNLFNIIKVFELSSGLNINFSK 344
             E+K  +KG  + S  +   LFADD+++++++       L  +IK +   SG  IN  K
Sbjct: 680 --EEKA-IKGIHIGSEEIKLSLFADDMIVYLENTRDSTTKLLEVIKEYSNVSGYKINTHK 739

Query: 345 SSITGINVEDSRVAQIAANWGCPTTQFP--IPYLGSPLGGNPSS--SSFWANTVDKIHRK 404
           S        ++  A+       P T  P  + YLG  L  +        +     +I   
Sbjct: 740 S--VAFIYTNNNQAEKTVKDSIPFTVVPKKMKYLGVYLTKDVKDLYKENYETLRKEIAED 799

Query: 405 LDSWRYSYISKGGRLTLIRATL--SGIPNYLLSIFKAPQSVCFSIDKIIRSFLWH 432
           ++ W+    S  GR+ +++ ++    I N+     KAP S    ++KII  F+W+
Sbjct: 800 VNKWKNIPCSWLGRINIVKMSILPKAIYNFNAIPIKAPLSYFKDLEKIILHFIWN 849

BLAST of Lag0000342 vs. ExPASy Swiss-Prot
Match: P14381 (Transposon TX1 uncharacterized 149 kDa protein OS=Xenopus laevis OX=8355 PE=4 SV=1)

HSP 1 Score: 132.9 bits (333), Expect = 1.1e-29
Identity = 120/417 (28.78%), Postives = 191/417 (45.80%), Query Frame = 0

Query: 56  PFSELEVFQNLKAMGHNKSPGPDGFTVEFFKKSWIAIRPSVMEVFRDFFQKGIINCNVNE 115
           P +  E+ Q L+ M HNKSPG DG T+EFF+  W  + P    V  + F+KG +  +   
Sbjct: 448 PITLDELSQALRLMPHNKSPGLDGLTIEFFQFFWDTLGPDFHRVLTEAFKKGELPLSCRR 507

Query: 116 TYITLIPKKNKAMQIQDFRPISLTTVLYRLIAKTLAERLKSTLQGTISENQLAFVKGRQI 175
             ++L+PKK     I+++RP+SL +  Y+++AK ++ RLKS L   I  +Q   V GR I
Sbjct: 508 AVLSLLPKKGDLRLIKNWRPVSLLSTDYKIVAKAISLRLKSVLAEVIHPDQSYTVPGRTI 567

Query: 176 TDAILVANEAIDFWKCSRTRGFVIKLDIEKAFDKICWNFVDKIL-------AFKGY---- 235
            D + +  + + F + +      + LD EKAFD++   ++   L        F GY    
Sbjct: 568 FDNVFLIRDLLHFARRTGLSLAFLSLDQEKAFDRVDHQYLIGTLQAYSFGPQFVGYLKTM 627

Query: 236 --------PITWP-KRKIKAERGIRQGDPISPFIFVLAMDYLSRILQSAEQKGLVKGCSL 295
                    I W     +   RG+RQG P+S  ++ LA++    +L+      ++K   +
Sbjct: 628 YASAECLVKINWSLTAPLAFGRGVRQGCPLSGQLYSLAIEPFLCLLRKRLTGLVLKEPDM 687

Query: 296 NSVSVSHLLFADDILLFVQDNDAMLGNLFNIIKVFELSSGLNINFSKSS--ITG-INVED 355
             V  +   +ADD++L  QD    L       +V+  +S   IN+SKSS  + G + V+ 
Sbjct: 688 RVVLSA---YADDVILVAQDL-VDLERAQECQEVYAAASSARINWSKSSGLLEGSLKVDF 747

Query: 356 SRVAQIAANWGCPTTQFPIPYLGSPLGGN--PSSSSFWANTVDKIHRKLDSWR--YSYIS 415
              A    +W        I YLG  L     P S +F     + +  +L  W+     +S
Sbjct: 748 LPPAFRDISWESKI----IKYLGVYLSAEEYPVSQNF-IELEECVLTRLGKWKGFAKVLS 807

Query: 416 KGGRLTLIRATLSGIPNYLLSIFKAPQSVCFSIDKIIRSFLWHG-HDQS---SSIPL 442
             GR  +I   ++    Y L      Q     I + +  FLW G H  S   SS+PL
Sbjct: 808 MRGRALVINQLVASQIWYRLICLSPTQEFIAKIQRRLLDFLWIGKHWVSAGVSSLPL 855

BLAST of Lag0000342 vs. ExPASy Swiss-Prot
Match: P11369 (LINE-1 retrotransposable element ORF2 protein OS=Mus musculus OX=10090 GN=Pol PE=1 SV=2)

HSP 1 Score: 131.7 bits (330), Expect = 2.6e-29
Identity = 117/454 (25.77%), Postives = 193/454 (42.51%), Query Frame = 0

Query: 4   IRMWKQRCKKTWLKEGDENTTFFHKGTTSEGWMVSNLNWCPISGPEATSLIQPFSELEVF 63
           IR + +R   T L+  DE   F  +      + V  LN       +   L  P S  E+ 
Sbjct: 418 IRSFYKRLYSTKLENLDEMDKFLDR------YQVPKLN-----QDQVDHLNSPISPKEIE 477

Query: 64  QNLKAMGHNKSPGPDGFTVEFFKKSWIAIRPSVMEVFRDFFQKGIINCNVNETYITLIPK 123
             + ++   KSPGPDGF+ EF++     + P + ++F     +G +  +  E  ITLIPK
Sbjct: 478 AVINSLPTKKSPGPDGFSAEFYQTFKEDLIPILHKLFHKIEVEGTLPNSFYEATITLIPK 537

Query: 124 KNK-AMQIQDFRPISLTTVLYRLIAKTLAERLKSTLQGTISENQLAFVKGRQITDAILVA 183
             K   +I++FRPISL  +  +++ K LA R++  ++  I  +Q+ F+ G Q    I  +
Sbjct: 538 PQKDPTKIENFRPISLMNIDAKILNKILANRIQEHIKAIIHPDQVGFIPGMQGWFNIRKS 597

Query: 184 NEAIDFW-KCSRTRGFVIKLDIEKAFDKICWNFVDKILAFKG------------YPITWP 243
              I +  K       +I LD EKAFDKI   F+ K+L   G            Y     
Sbjct: 598 INVIHYINKLKDKNHMIISLDAEKAFDKIQHPFMIKVLERSGIQGPYLNMIKAIYSKPVA 657

Query: 244 KRKIKAER--------GIRQGDPISPFIFVLAMDYLSRILQSAEQKGLVKGCSLNSVSVS 303
             K+  E+        G RQG P+SP++F + ++ L+R ++  ++   +KG  +    V 
Sbjct: 658 NIKVNGEKLEAIPLKSGTRQGCPLSPYLFNIVLEVLARAIRQQKE---IKGIQIGKEEVK 717

Query: 304 HLLFADDILLFVQDNDAMLGNLFNIIKVFELSSGLNINFSKSSITGINVEDSRVAQIAAN 363
             L ADD+++++ D       L N+I  F    G  IN +KS             +I   
Sbjct: 718 ISLLADDMIVYISDPKNSTRELLNLINSFGEVVGYKINSNKSMAFLYTKNKQAEKEIRET 777

Query: 364 WGCPTTQFPIPYLGSPLGGNPSS--SSFWANTVDKIHRKLDSWRYSYISKGGRLTLIRAT 423
                    I YLG  L           + +   +I   L  W+    S  GR+ +++  
Sbjct: 778 TPFSIVTNNIKYLGVTLTKEVKDLYDKNFKSLKKEIKEDLRRWKDLPCSWIGRINIVKMA 837

Query: 424 L--SGIPNYLLSIFKAPQSVCFSIDKIIRSFLWH 432
           +    I  +     K P      ++  I  F+W+
Sbjct: 838 ILPKAIYRFNAIPIKIPTQFFNELEGAICKFVWN 857

BLAST of Lag0000342 vs. ExPASy Swiss-Prot
Match: Q03274 (Retrovirus-related Pol polyprotein from type-1 retrotransposable element R2 (Fragment) OS=Popillia japonica OX=7064 PE=4 SV=1)

HSP 1 Score: 89.4 bits (220), Expect = 1.5e-16
Identity = 77/275 (28.00%), Postives = 129/275 (46.91%), Query Frame = 0

Query: 74  SPGPDGFTVEFFKKSWIAIRPSVMEVFRDFFQKGIINCNVNETY----ITLIPKKNKAMQ 133
           +PG DG TV+   ++ +          R+F Q  ++  +V   +     TLIPK      
Sbjct: 22  APGSDGLTVQAITRTRLP---------RNFVQLHLLRGHVPTPWTAMRTTLIPKDGDLEN 81

Query: 134 IQDFRPISLTTVLYRLIAKTLAERLKSTLQGTISENQLAFVKGRQITDAILVANEAIDFW 193
             ++RPI++ + L RL+ + LA+RL++ ++   ++   A + G  +   +L  +  I   
Sbjct: 82  PSNWRPITIASALQRLLHRILAKRLEAAVELHPAQKGYARIDGTLVNSLLL--DTYISSR 141

Query: 194 KCSRTRGFVIKLDIEKAFDKICWNFVDKILAFKGYP------ITW--------------- 253
           +  R    V+ LD+ KAFD +  + + + L   G        IT                
Sbjct: 142 REQRKTYNVVSLDVRKAFDTVSHSSICRALQRLGIDEGTSNYITGSLSDSTTTIRVGPGS 201

Query: 254 PKRKIKAERGIRQGDPISPFIFVLAMDYLSRILQSAEQKGLVKGCSLNSVSVSHLLFADD 313
             RKI   RG++QGDP+SPF+F   +D L   LQS    G+  G ++    +  L FADD
Sbjct: 202 QTRKICIRRGVKQGDPLSPFLFNAVLDELLCSLQST--PGI--GGTIGEEKIPVLAFADD 261

Query: 314 ILLFVQDNDAMLGNLFNIIKVFELSSGLNINFSKS 324
           +LL ++DND +L      +  F    G+++N  KS
Sbjct: 262 LLL-LEDNDVLLPTTLATVANFFRLRGMSLNAKKS 280

BLAST of Lag0000342 vs. ExPASy TrEMBL
Match: A0A5D3DM72 (LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold266G002870 PE=4 SV=1)

HSP 1 Score: 493.4 bits (1269), Expect = 1.2e-135
Identity = 252/524 (48.09%), Postives = 338/524 (64.50%), Query Frame = 0

Query: 2   QEIRMWKQRCKKTWLKEGDENTTFFHK--------------------------------- 61
           +E ++W Q+ K+ W+ EGDENT+FFHK                                 
Sbjct: 181 KEAQIWYQKSKRLWITEGDENTSFFHKICSARQRRSIISNINSVDGVPCSTNESIAKAFL 240

Query: 62  ---------GTTSEGWMVSNLNWCPISGPEATSLIQPFSELEVFQNLKAMGHNKSPGPDG 121
                    G     W++ NLNW PIS  +A +L   F+E E+ + L A  +NKSPGPDG
Sbjct: 241 DHFEDIYKGGGEESPWLIDNLNWSPISTNQAQNLCSMFTEEEIHEALTAFSNNKSPGPDG 300

Query: 122 FTVEFFKKSWIAIRPSVMEVFRDFFQKGIINCNVNETYITLIPKKNKAMQIQDFRPISLT 181
           FT+EF+K +W  ++  ++ +FRDF    IIN  VN T I LI KK K  +  D+RPISLT
Sbjct: 301 FTMEFYKSTWSVLKEEILNIFRDFHSNCIINKAVNITNIALIAKKEKCAEPADYRPISLT 360

Query: 182 TVLYRLIAKTLAERLKSTLQGTISENQLAFVKGRQITDAILVANEAIDFWKCSRTRGFVI 241
           T +Y+LIAK +AERLK TL  T++ENQ+AFVKGRQI DAILVANEAID+W+  + +GFVI
Sbjct: 361 TSIYKLIAKVIAERLKDTLPYTVAENQMAFVKGRQIIDAILVANEAIDYWRVKKIQGFVI 420

Query: 242 KLDIEKAFDKICWNFVDKILAFKGYPITW--------------------PKRKIKAERGI 301
           KLDIEKAFDK+ W F+D +L  KGYP  W                    P+ KI+  RGI
Sbjct: 421 KLDIEKAFDKLNWRFIDFMLMKKGYPFKWRNWIRACISSVQYSIIINGRPRGKIQPSRGI 480

Query: 302 RQGDPISPFIFVLAMDYLSRILQSAEQKGLVKGCSL-NSVSVSHLLFADDILLFVQDNDA 361
           RQGDPISPFIFVLAMDY+SR+L S  +K  +KG  L  +++++HLLFADDILLFV+D++ 
Sbjct: 481 RQGDPISPFIFVLAMDYMSRLLNSVGEK--IKGVKLEGNINLTHLLFADDILLFVEDDEH 540

Query: 362 MLGNLFNIIKVFELSSGLNINFSKSSITGINVEDSRVAQIAANWGCPTTQFPIPYLGSPL 421
            + NL NII +F+L+SGL+IN +KS+I+ INV+ SR  QIA+ WG  T   PI YLG PL
Sbjct: 541 SIQNLKNIINLFQLASGLSINLNKSTISPINVDASRTEQIASQWGISTKFLPINYLGVPL 600

Query: 422 GGNPSSSSFWANTVDKIHRKLDSWRYSYISKGGRLTLIRATLSGIPNYLLSIFKAPQSVC 463
           GG   + +FW N  +KI++KL SW+YS +SKGG++TLI+++L+ +P Y LSIFKAP S C
Sbjct: 601 GGKQITKTFWKNVEEKINKKLASWKYSMLSKGGKITLIKSSLASLPTYQLSIFKAPVSTC 660

BLAST of Lag0000342 vs. ExPASy TrEMBL
Match: A0A5A7TI93 (LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold206G00120 PE=4 SV=1)

HSP 1 Score: 493.0 bits (1268), Expect = 1.6e-135
Identity = 250/524 (47.71%), Postives = 339/524 (64.69%), Query Frame = 0

Query: 2   QEIRMWKQRCKKTWLKEGDENTTFFHK--------------------------------- 61
           +E ++W Q+ K+ W+ EGDENT+FFHK                                 
Sbjct: 5   KEAQIWYQKSKRLWITEGDENTSFFHKICTARQRRSIISNIKSVDGVPCSTNESIAKAFL 64

Query: 62  ---------GTTSEGWMVSNLNWCPISGPEATSLIQPFSELEVFQNLKAMGHNKSPGPDG 121
                    G     W++ NL+W PIS  +A +L   F+E E+   L A  +NKSPGPDG
Sbjct: 65  DHFEDIYKGGGEESPWLIDNLSWSPISTTQAQNLCSCFTEEEIHAALTAFSNNKSPGPDG 124

Query: 122 FTVEFFKKSWIAIRPSVMEVFRDFFQKGIINCNVNETYITLIPKKNKAMQIQDFRPISLT 181
           FT+EF+K +W  ++  ++ +FRDF    IIN  VN T I LI KK K  +  D+RPISLT
Sbjct: 125 FTMEFYKSTWSDLKEEILNIFRDFHSNCIINKAVNITNIALIAKKEKCAEPADYRPISLT 184

Query: 182 TVLYRLIAKTLAERLKSTLQGTISENQLAFVKGRQITDAILVANEAIDFWKCSRTRGFVI 241
           T +Y+LIAK +AERLK TL  T++ENQ+AFVKGRQI DAILVANEAID+W+  + +GFVI
Sbjct: 185 TSIYKLIAKVIAERLKETLPSTVAENQMAFVKGRQIIDAILVANEAIDYWRVKKIQGFVI 244

Query: 242 KLDIEKAFDKICWNFVDKILAFKGYPITW--------------------PKRKIKAERGI 301
           KLDIEKAFDK+ W F+D +L  KGYP  W                    P+ KI+  RGI
Sbjct: 245 KLDIEKAFDKLNWRFIDFMLMKKGYPFRWRRWIRACISSVQYSIIINGRPRGKIQPSRGI 304

Query: 302 RQGDPISPFIFVLAMDYLSRILQSAEQKGLVKGCSL-NSVSVSHLLFADDILLFVQDNDA 361
           RQGDPISPFIFVLAMDY+SR+L S  +K  +KG  +  +++++HLLFADDILLFV+D++ 
Sbjct: 305 RQGDPISPFIFVLAMDYISRLLNSVGEK--IKGVKMEGNINLTHLLFADDILLFVEDDEH 364

Query: 362 MLGNLFNIIKVFELSSGLNINFSKSSITGINVEDSRVAQIAANWGCPTTQFPIPYLGSPL 421
            + NL NII +F+L+SGL+IN +KS+I+ INV+ +R  QIA+ WG  T   PI YLG PL
Sbjct: 365 SIQNLKNIINLFQLASGLSINLNKSTISPINVDAARTEQIASQWGISTKFLPINYLGVPL 424

Query: 422 GGNPSSSSFWANTVDKIHRKLDSWRYSYISKGGRLTLIRATLSGIPNYLLSIFKAPQSVC 463
           GG  ++ +FW N  +KI++KL SW+YS +SKGG++TLI+++L+ +P Y LSIFKAP S C
Sbjct: 425 GGKQTTKTFWKNVEEKINKKLASWKYSMLSKGGKITLIKSSLASLPTYQLSIFKAPVSAC 484

BLAST of Lag0000342 vs. ExPASy TrEMBL
Match: A0A1S4E2K5 (LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo OX=3656 GN=LOC107991687 PE=4 SV=1)

HSP 1 Score: 491.9 bits (1265), Expect = 3.6e-135
Identity = 251/524 (47.90%), Postives = 337/524 (64.31%), Query Frame = 0

Query: 2   QEIRMWKQRCKKTWLKEGDENTTFFHK--------------------------------- 61
           +E ++W Q+ K+ W+ EGDENT+FFHK                                 
Sbjct: 181 KEAQIWYQKSKRLWITEGDENTSFFHKICSARQRRSIISNINSVDGVPCSTNESIAKAFL 240

Query: 62  ---------GTTSEGWMVSNLNWCPISGPEATSLIQPFSELEVFQNLKAMGHNKSPGPDG 121
                    G     W++ NLNW PIS  +A +L   F+E E+ + L A  +NKSPGPDG
Sbjct: 241 DHFEDIYKGGGEESPWLIDNLNWSPISTNQAQNLCSMFTEEEIHEALTAFSNNKSPGPDG 300

Query: 122 FTVEFFKKSWIAIRPSVMEVFRDFFQKGIINCNVNETYITLIPKKNKAMQIQDFRPISLT 181
           FT+EF+K +W  ++  ++ +FRDF    IIN  VN T I LI KK K  +  D+RPISLT
Sbjct: 301 FTMEFYKSTWSVLKEEILNIFRDFHSNCIINKAVNITNIALIAKKEKCAEPADYRPISLT 360

Query: 182 TVLYRLIAKTLAERLKSTLQGTISENQLAFVKGRQITDAILVANEAIDFWKCSRTRGFVI 241
           T +Y+LIAK +AERLK TL  T++ENQ+AFVKGRQI DAILVANEAID+W+  + +GFVI
Sbjct: 361 TSIYKLIAKVIAERLKDTLPYTVAENQMAFVKGRQIIDAILVANEAIDYWRVKKIQGFVI 420

Query: 242 KLDIEKAFDKICWNFVDKILAFKGYPITW--------------------PKRKIKAERGI 301
           KLDIEKAFDK+ W F+D +L  KGYP  W                    P+ KI+  RGI
Sbjct: 421 KLDIEKAFDKLNWRFIDFMLMKKGYPFKWRNWIRACISSVQYSIIINGRPRGKIQPSRGI 480

Query: 302 RQGDPISPFIFVLAMDYLSRILQSAEQKGLVKGCSL-NSVSVSHLLFADDILLFVQDNDA 361
           RQGDPISPFIFVLAMDY+SR+L S  +K  +KG  L  +++++HLLFADDILLFV+D++ 
Sbjct: 481 RQGDPISPFIFVLAMDYMSRLLNSVGEK--IKGVKLEGNINLTHLLFADDILLFVEDDEH 540

Query: 362 MLGNLFNIIKVFELSSGLNINFSKSSITGINVEDSRVAQIAANWGCPTTQFPIPYLGSPL 421
            + NL NII +F+L+SGL+IN +KS+I+ INV+ SR  QIA+ WG  T   PI YLG PL
Sbjct: 541 SIQNLKNIINLFQLASGLSINLNKSTISPINVDASRTEQIASQWGISTKFLPINYLGVPL 600

Query: 422 GGNPSSSSFWANTVDKIHRKLDSWRYSYISKGGRLTLIRATLSGIPNYLLSIFKAPQSVC 463
           GG   + +FW N  +KI++KL SW+YS +SKGG++TLI+++L+ +P Y LSIFK P S C
Sbjct: 601 GGKQITKTFWKNVEEKINKKLASWKYSMLSKGGKITLIKSSLASLPTYQLSIFKVPVSTC 660

BLAST of Lag0000342 vs. ExPASy TrEMBL
Match: A0A5A7TTK1 (LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold216G00450 PE=4 SV=1)

HSP 1 Score: 489.6 bits (1259), Expect = 1.8e-134
Identity = 241/524 (45.99%), Postives = 330/524 (62.98%), Query Frame = 0

Query: 1   MQEIRMWKQRCKKTWLKEGDENTTFFHK-------------------------------- 60
           ++E + W QR KK WL+EGDEN++FFH+                                
Sbjct: 180 LKESQFWYQRAKKLWLREGDENSSFFHRICTARQKRNFIQEIQDEEGLIQNSNDSISSAF 239

Query: 61  ----------GTTSEGWMVSNLNWCPISGPEATSLIQPFSELEVFQNLKAMGHNKSPGPD 120
                      T S+ + + NL W PI   E  +L  PF E E+   + ++   K+PGPD
Sbjct: 240 IKFFSRIFRGPTKSDPFFIDNLEWNPIEHSEGLNLCAPFLEDEIKGVINSLDGKKTPGPD 299

Query: 121 GFTVEFFKKSWIAIRPSVMEVFRDFFQKGIINCNVNETYITLIPKKNKAMQIQDFRPISL 180
           GF + FFK  W  ++  +M++F+DF+ KG+IN N+N TYI LIPKK      +DFRPISL
Sbjct: 300 GFPISFFKSYWYLLKEDIMDIFKDFYDKGVINKNMNNTYIALIPKKKDFSNPKDFRPISL 359

Query: 181 TTVLYRLIAKTLAERLKSTLQGTISENQLAFVKGRQITDAILVANEAIDFWKCSRTRGFV 240
           TT +Y++IAKTL+ RLK++L  TISENQLAFVK RQITDAIL+ANEA+DFWK  + +GF+
Sbjct: 360 TTSIYKIIAKTLSNRLKTSLPNTISENQLAFVKNRQITDAILMANEAVDFWKVKKIKGFI 419

Query: 241 IKLDIEKAFDKICWNFVDKILAFKGYPITW--------------------PKRKIKAERG 300
           +KLDIEKAFD + W+F+D +L  K +PI W                    P+ +IKA RG
Sbjct: 420 LKLDIEKAFDNLNWDFIDFVLEKKNFPILWRKWIRGCISNVTYSIIVNGRPQGRIKANRG 479

Query: 301 IRQGDPISPFIFVLAMDYLSRILQSAEQKGLVKGCSLNS-VSVSHLLFADDILLFVQDND 360
           +RQGDP+SPF+FV+AMDYLSR+L   E  G +KG S +S  ++SH+LFADDILLF++DND
Sbjct: 480 LRQGDPLSPFLFVIAMDYLSRLLSHLEITGAIKGVSFSSNCNISHILFADDILLFIEDND 539

Query: 361 AMLGNLFNIIKVFELSSGLNINFSKSSITGINVEDSRVAQIAANWGCPTTQFPIPYLGSP 420
             L NL   + +FE +SGL IN  KS++  +NV + R  + A+ WG  +   P+ YLG P
Sbjct: 540 FFLNNLRMALSLFERASGLKINLLKSALVPMNVSEIRAKECASFWGISSQSLPLSYLGVP 599

Query: 421 LGGNPSSSSFWANTVDKIHRKLDSWRYSYISKGGRLTLIRATLSGIPNYLLSIFKAPQSV 462
           LGGNP S  FW+N  +KI +KL++W+Y+ ISKGGRLTLI++TLS +P Y LS+F+AP   
Sbjct: 600 LGGNPKSRLFWSNVEEKIQKKLNNWKYAQISKGGRLTLIKSTLSSLPTYQLSVFQAPSLT 659

BLAST of Lag0000342 vs. ExPASy TrEMBL
Match: A0A5D3BUZ3 (LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold808G00050 PE=4 SV=1)

HSP 1 Score: 483.8 bits (1244), Expect = 9.8e-133
Identity = 243/524 (46.37%), Postives = 326/524 (62.21%), Query Frame = 0

Query: 1   MQEIRMWKQRCKKTWLKEGDENTTFFHK-------------------------------- 60
           ++E + W QR KK WL+EGDEN++FFH+                                
Sbjct: 180 LKESQFWYQRAKKLWLREGDENSSFFHRICSSRQKRSFIHEIQDEEGLIQNTNNGISTAF 239

Query: 61  ----------GTTSEGWMVSNLNWCPISGPEATSLIQPFSELEVFQNLKAMGHNKSPGPD 120
                      T S+   + NL+W PI   E   L  PF E E+   + ++   K+PGPD
Sbjct: 240 IKFFSRIYRSSTKSDPLFIDNLDWNPIEHSEWPHLCAPFLEGEIKGVINSLDGKKTPGPD 299

Query: 121 GFTVEFFKKSWIAIRPSVMEVFRDFFQKGIINCNVNETYITLIPKKNKAMQIQDFRPISL 180
           GF + FFK  W  ++  +M++F+DF+ KG+IN N+N TYI LIPKK      +DFRPISL
Sbjct: 300 GFPISFFKSYWYLLKEDIMDIFKDFYDKGVINKNMNNTYIALIPKKKNYSHPKDFRPISL 359

Query: 181 TTVLYRLIAKTLAERLKSTLQGTISENQLAFVKGRQITDAILVANEAIDFWKCSRTRGFV 240
           TT +Y++IAKTL+ RLK+ L  TIS NQLAFVK RQITDAIL+ANEA+DFWK  + +GF+
Sbjct: 360 TTSIYKIIAKTLSNRLKTALPDTISGNQLAFVKNRQITDAILMANEALDFWKVKKIKGFI 419

Query: 241 IKLDIEKAFDKICWNFVDKILAFKGYPITW--------------------PKRKIKAERG 300
           +KLDIEKAFD + W+F+D +L  K +P  W                    P+ +IKA RG
Sbjct: 420 LKLDIEKAFDNLNWDFIDFVLEKKNFPNLWRKWIRGCISNVTYSVIVNGRPQGRIKANRG 479

Query: 301 IRQGDPISPFIFVLAMDYLSRILQSAEQKGLVKGCSLNS-VSVSHLLFADDILLFVQDND 360
           +RQGDP+SPF+FV+AMDYLSR+L   E  G +KG SLNS  ++SH+LFADDILLF++DND
Sbjct: 480 LRQGDPLSPFLFVIAMDYLSRLLSHLESSGAIKGVSLNSNCNISHILFADDILLFIEDND 539

Query: 361 AMLGNLFNIIKVFELSSGLNINFSKSSITGINVEDSRVAQIAANWGCPTTQFPIPYLGSP 420
             L NL   + +FE +SGL IN  KS++  +NV  +R  + A+ WG      P+ YLG P
Sbjct: 540 YFLKNLRMALSLFEKASGLKINLLKSALVPVNVSVNRAKECASFWGISCHSLPLSYLGVP 599

Query: 421 LGGNPSSSSFWANTVDKIHRKLDSWRYSYISKGGRLTLIRATLSGIPNYLLSIFKAPQSV 462
           LGGNP S+ FW N  DKI +KL++W+Y+ ISKGGRLTLI++TLS +P Y LS+F+ P   
Sbjct: 600 LGGNPKSNLFWRNVEDKIQKKLNNWKYAQISKGGRLTLIKSTLSSLPIYQLSVFQDPSLT 659

BLAST of Lag0000342 vs. TAIR 10
Match: AT1G43760.1 (DNAse I-like superfamily protein )

HSP 1 Score: 84.3 bits (207), Expect = 3.3e-16
Identity = 56/186 (30.11%), Postives = 86/186 (46.24%), Query Frame = 0

Query: 7   WKQRCKKTWLKEGDENTTFFHKGTTS-------------EGWMVSNLN---------WCP 66
           ++Q+ +  WL++GD NT FFHK   +             +   V N+          +  
Sbjct: 435 YRQKSRIKWLQDGDANTRFFHKVILANQAKNLIKFLRMDDDVRVENVTQVKEMIVAYYTH 494

Query: 67  ISGPEATSL----------IQPF--------------SELEVFQNLKAMGHNKSPGPDGF 126
           + G ++  L          I PF              S+ E+   + AM  NK+PGPD F
Sbjct: 495 LLGSDSDILTPDSVQRIKDIHPFRCNDTLASRLSALPSDKEITAAVFAMPRNKAPGPDSF 554

Query: 127 TVEFFKKSWIAIRPSVMEVFRDFFQKGIINCNVNETYITLIPKKNKAMQIQDFRPISLTT 147
           T EFF +SW  ++ S +   ++FF+ G +    N T ITLIPK     Q+  FRP+S  T
Sbjct: 555 TAEFFWESWFVVKDSTIAAVKEFFRTGHLLKRFNATAITLIPKVTGVDQLSMFRPVSCCT 614

BLAST of Lag0000342 vs. TAIR 10
Match: ATMG01250.1 (RNA-directed DNA polymerase (reverse transcriptase) )

HSP 1 Score: 60.8 bits (146), Expect = 3.9e-09
Identity = 27/62 (43.55%), Postives = 43/62 (69.35%), Query Frame = 0

Query: 229 PKRKIKAERGIRQGDPISPFIFVLAMDYLSRILQSAEQKGLVKG--CSLNSVSVSHLLFA 288
           P+  +   RG+RQGDP+SP++F+L  + LS + + A+++G + G   S NS  ++HLLFA
Sbjct: 18  PQGLVTPSRGLRQGDPLSPYLFILCTEVLSGLCRRAQEQGRLPGIRVSNNSPRINHLLFA 77

BLAST of Lag0000342 vs. TAIR 10
Match: AT4G20520.1 (RNA binding;RNA-directed DNA polymerases )

HSP 1 Score: 60.1 bits (144), Expect = 6.7e-09
Identity = 32/99 (32.32%), Postives = 53/99 (53.54%), Query Frame = 0

Query: 150 LAERLKSTLQGTISENQLAFVKGRQITDAILVANEAIDFWKCSRTRG----FVIKLDIEK 209
           + ERLK  +   I   Q +F+ GR  TD I+   EA+   +  R +G     ++KLD+EK
Sbjct: 1   MVERLKPLMTNLIGPAQASFIPGRVSTDNIVFVQEAVHSMR--RKKGVKGWMLLKLDLEK 60

Query: 210 AFDKICWNFVDKILAFKGYPITWPKRKIKAERGIRQGDP 245
           A+D+I W++++  L   G+P  W     ++  G R+  P
Sbjct: 61  AYDRIRWDYLEDTLISAGFPEVWLPEIARSTFGARRVAP 97

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAA0039770.12.6e-13548.09LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa] >TYK247... [more]
KAA0041367.13.3e-13547.71LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa][more]
XP_016902461.17.4e-13547.90PREDICTED: LINE-1 retrotransposable element ORF2 protein [Cucumis melo][more]
KAA0046762.13.7e-13445.99LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa][more]
KAA0037445.12.0e-13246.37LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa] >TYK019... [more]
Match NameE-valueIdentityDescription
O003702.5e-3226.41LINE-1 retrotransposable element ORF2 protein OS=Homo sapiens OX=9606 PE=1 SV=1[more]
P085481.2e-3126.51LINE-1 reverse transcriptase homolog OS=Nycticebus coucang OX=9470 PE=4 SV=1[more]
P143811.1e-2928.78Transposon TX1 uncharacterized 149 kDa protein OS=Xenopus laevis OX=8355 PE=4 SV... [more]
P113692.6e-2925.77LINE-1 retrotransposable element ORF2 protein OS=Mus musculus OX=10090 GN=Pol PE... [more]
Q032741.5e-1628.00Retrovirus-related Pol polyprotein from type-1 retrotransposable element R2 (Fra... [more]
Match NameE-valueIdentityDescription
A0A5D3DM721.2e-13548.09LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo var. makuwa OX=119... [more]
A0A5A7TI931.6e-13547.71LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo var. makuwa OX=119... [more]
A0A1S4E2K53.6e-13547.90LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo OX=3656 GN=LOC1079... [more]
A0A5A7TTK11.8e-13445.99LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo var. makuwa OX=119... [more]
A0A5D3BUZ39.8e-13346.37LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo var. makuwa OX=119... [more]
Match NameE-valueIdentityDescription
AT1G43760.13.3e-1630.11DNAse I-like superfamily protein [more]
ATMG01250.13.9e-0943.55RNA-directed DNA polymerase (reverse transcriptase) [more]
AT4G20520.16.7e-0932.32RNA binding;RNA-directed DNA polymerases [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000477Reverse transcriptase domainPFAMPF00078RVT_1coord: 121..355
e-value: 1.2E-36
score: 126.3
IPR000477Reverse transcriptase domainPROSITEPS50878RT_POLcoord: 102..358
score: 19.596594
NoneNo IPR availablePANTHERPTHR33116REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED-RELATEDcoord: 49..454
NoneNo IPR availablePANTHERPTHR33116:SF38OS01G0158850 PROTEINcoord: 49..454
NoneNo IPR availableCDDcd01650RT_nLTR_likecoord: 115..358
e-value: 1.07013E-60
score: 198.284
IPR043502DNA/RNA polymerase superfamilySUPERFAMILY56672DNA/RNA polymerasescoord: 55..327

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0000342.1Lag0000342.1mRNA