Lag0000336 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0000336
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Retrovirus-related Pol polyprotein from transposon TNT 1-94
Location	chr4: 4528106 .. 4528681 (+)
RNA-Seq Expression	Lag0000336
Synteny	Lag0000336

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTGGAAGGTTCAAATTGAATTCGCACTTGAAGGACATGATCTAGAAAATTTCATTAACAGTGATGTTGAACCTCCACCTAAAAGAATCTCGGTATCGGAAAATTCATCTGCTACTAAAGTTAATCCAGAATATACCAAATGGAAGAAACAAGATCGGTTAATTTCTTCTTGGTTACTTGGCTCAATGACTGAAAATATATTGGAACAAGTCATTCACTGTAAGTCTTCTAGAGAAATTTGGATGTGTTTACTTCAGATTTTTAATTCAAAGAATAAGGCACAAGTGATGAGGATGAAAAATAGGCTTCAGACTATTCAAAAAGGAGGAATGTCGCTTAGTGAATATTTCTCCCAGATTAAGAAGTGCATTGATGCACTGGCTGCAATAGGAAAGACTATTGATGAAGAAGATCATGTTCTTTATATACTCGGCGGTTTGGGTCCAGACTATGAGTCCATGGTTTCTGCAATCACTGCAACTACTGAAGATCAAAGTGTTCAAGAAGTCATGGCACTTCTCTTAACTCATGAGAACCAGATTGAAAGAAAACTGAAGTCTATTGAGCAAATTTGA

mRNA sequence

ATGTGGAAGGTTCAAATTGAATTCGCACTTGAAGGACATGATCTAGAAAATTTCATTAACAGTGATGTTGAACCTCCACCTAAAAGAATCTCGGTATCGGAAAATTCATCTGCTACTAAAGTTAATCCAGAATATACCAAATGGAAGAAACAAGATCGGTTAATTTCTTCTTGGTTACTTGGCTCAATGACTGAAAATATATTGGAACAAGTCATTCACTGTAAGTCTTCTAGAGAAATTTGGATGTGTTTACTTCAGATTTTTAATTCAAAGAATAAGGCACAAGTGATGAGGATGAAAAATAGGCTTCAGACTATTCAAAAAGGAGGAATGTCGCTTAGTGAATATTTCTCCCAGATTAAGAAGTGCATTGATGCACTGGCTGCAATAGGAAAGACTATTGATGAAGAAGATCATGTTCTTTATATACTCGGCGGTTTGGGTCCAGACTATGAGTCCATGGTTTCTGCAATCACTGCAACTACTGAAGATCAAAGTGTTCAAGAAGTCATGGCACTTCTCTTAACTCATGAGAACCAGATTGAAAGAAAACTGAAGTCTATTGAGCAAATTTGA

Coding sequence (CDS)

ATGTGGAAGGTTCAAATTGAATTCGCACTTGAAGGACATGATCTAGAAAATTTCATTAACAGTGATGTTGAACCTCCACCTAAAAGAATCTCGGTATCGGAAAATTCATCTGCTACTAAAGTTAATCCAGAATATACCAAATGGAAGAAACAAGATCGGTTAATTTCTTCTTGGTTACTTGGCTCAATGACTGAAAATATATTGGAACAAGTCATTCACTGTAAGTCTTCTAGAGAAATTTGGATGTGTTTACTTCAGATTTTTAATTCAAAGAATAAGGCACAAGTGATGAGGATGAAAAATAGGCTTCAGACTATTCAAAAAGGAGGAATGTCGCTTAGTGAATATTTCTCCCAGATTAAGAAGTGCATTGATGCACTGGCTGCAATAGGAAAGACTATTGATGAAGAAGATCATGTTCTTTATATACTCGGCGGTTTGGGTCCAGACTATGAGTCCATGGTTTCTGCAATCACTGCAACTACTGAAGATCAAAGTGTTCAAGAAGTCATGGCACTTCTCTTAACTCATGAGAACCAGATTGAAAGAAAACTGAAGTCTATTGAGCAAATTTGA

Protein sequence

MWKVQIEFALEGHDLENFINSDVEPPPKRISVSENSSATKVNPEYTKWKKQDRLISSWLLGSMTENILEQVIHCKSSREIWMCLLQIFNSKNKAQVMRMKNRLQTIQKGGMSLSEYFSQIKKCIDALAAIGKTIDEEDHVLYILGGLGPDYESMVSAITATTEDQSVQEVMALLLTHENQIERKLKSIEQI

Homology

[Close]

BLAST of Lag0000336 vs. NCBI nr
Match: KAA0048297.1 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Cucumis melo var. makuwa])

HSP 1 Score: 215.7 bits (548), Expect = 3.5e-52
Identity = 109/190 (57.37%), Postives = 141/190 (74.21%), Query Frame = 0


Query: 1   MWKVQIEFALEGHDLENFINSDVEPPPKRISVSENSSATKV---NPEYTKWKKQDRLISS 60
           +WK QI  ALE +DLENF+ S+ EPP K +  +E+SSA+     NP Y  WK+QDRLISS
Sbjct: 41  LWKFQILTALEAYDLENFLESESEPPSKYLISTESSSASATGTPNPAYKVWKRQDRLISS 100

Query: 61  WLLGSMTENILEQVIHCKSSREIWMCLLQIFNSKNKAQVMRMKNRLQTIQKGGMSLSEYF 120
           WLLGSM+E IL Q++HCKS++EIW  L  IF+S+  AQ M+ KN+L  I+KG M L EYF
Sbjct: 101 WLLGSMSEEILNQMLHCKSAKEIWETLQGIFSSRYLAQAMQFKNKLHNIKKGSMPLKEYF 160

Query: 121 SQIKKCIDALAAIGKTIDEEDHVLYILGGLGPDYESMVSAITATTEDQSVQEVMALLLTH 180
            +I +C+DALA+I K +  +DH+LYIL GLG DY+SM+S I+A T+  SVQEVM+LLLT 
Sbjct: 161 LKILQCVDALASINKPVSSDDHILYILAGLGSDYQSMISVISARTDSPSVQEVMSLLLTQ 220

Query: 181 ENQIERKLKS 188
           E+Q E KL S
Sbjct: 221 ESQNESKLIS 230

[Close]

BLAST of Lag0000336 vs. NCBI nr
Match: TYK10642.1 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Cucumis melo var. makuwa])

HSP 1 Score: 215.7 bits (548), Expect = 3.5e-52
Identity = 109/190 (57.37%), Postives = 141/190 (74.21%), Query Frame = 0


Query: 1   MWKVQIEFALEGHDLENFINSDVEPPPKRISVSENSSATKV---NPEYTKWKKQDRLISS 60
           +WK QI  ALE +DLENF+ S+ EPP K +  +E+SSA+     NP Y  WK+QDRLISS
Sbjct: 41  LWKFQILTALEAYDLENFLESESEPPSKYLISTESSSASATGTPNPAYKVWKRQDRLISS 100

Query: 61  WLLGSMTENILEQVIHCKSSREIWMCLLQIFNSKNKAQVMRMKNRLQTIQKGGMSLSEYF 120
           WLLGSM+E IL Q++HCKS++EIW  L  IF+S+  AQ M+ KN+L  I+KG M L EYF
Sbjct: 101 WLLGSMSEEILNQMLHCKSAKEIWETLQGIFSSRYLAQAMQFKNKLHNIKKGSMPLKEYF 160

Query: 121 SQIKKCIDALAAIGKTIDEEDHVLYILGGLGPDYESMVSAITATTEDQSVQEVMALLLTH 180
            +I +C+DALA+I K +  +DH+LYIL GLG DY+SM+S I+A T+  SVQEVM+LLLT 
Sbjct: 161 LKILQCVDALASINKPVSSDDHILYILAGLGSDYQSMISVISARTDSPSVQEVMSLLLTQ 220

Query: 181 ENQIERKLKS 188
           E+Q E KL S
Sbjct: 221 ESQNESKLIS 230

[Close]

BLAST of Lag0000336 vs. NCBI nr
Match: KAA0053143.1 (keratin, type II cytoskeletal 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 210.7 bits (535), Expect = 1.1e-50
Identity = 107/190 (56.32%), Postives = 143/190 (75.26%), Query Frame = 0


Query: 1   MWKVQIEFALEGHDLENFINSDVEPPPKRISV--SENSSATKV-NPEYTKWKKQDRLISS 60
           +WK QI  ALE +DLENF  S++EPP K ++   S ++SAT+  NPEY  WK+ +RLIS 
Sbjct: 36  LWKFQILTALEAYDLENFFESELEPPSKYLTSTGSSSTSATRTPNPEYKVWKRHNRLISP 95

Query: 61  WLLGSMTENILEQVIHCKSSREIWMCLLQIFNSKNKAQVMRMKNRLQTIQKGGMSLSEYF 120
           WLLGSM+E IL Q++HCKS++EIW  L  IF+S+  AQ M+ KN+L  I+KG MSL EYF
Sbjct: 96  WLLGSMSEEILNQMVHCKSAKEIWGTLQGIFSSRYLAQAMQFKNKLHNIKKGSMSLKEYF 155

Query: 121 SQIKKCIDALAAIGKTIDEEDHVLYILGGLGPDYESMVSAITATTEDQSVQEVMALLLTH 180
            +I++C+DALA+I K +  +DH+LYIL GLG DY+SM+S I+A T+  S+QEVM+LLLT 
Sbjct: 156 LKIQQCVDALASINKPVSSDDHILYILVGLGYDYQSMISIISARTDSPSIQEVMSLLLTQ 215

Query: 181 ENQIERKLKS 188
           E+Q E KL S
Sbjct: 216 ESQNESKLIS 225

[Close]

BLAST of Lag0000336 vs. NCBI nr
Match: KAA0045111.1 (putative glutathione S-transferase isoform X1 [Cucumis melo var. makuwa] >TYK23627.1 putative glutathione S-transferase isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 192.2 bits (487), Expect = 4.1e-45
Identity = 98/195 (50.26%), Postives = 141/195 (72.31%), Query Frame = 0


Query: 4   VQIEFALEGHDLENFINSDVEPPPKRIS-------VSENSSATK----VNPEYTKWKKQD 63
           V+  F  E +DLE++++S+ EPP K I+       V+  SS+      +NPEY  WK+QD
Sbjct: 171 VEEAFGFEAYDLESYLDSNAEPPIKYINQTPHQSLVAAGSSSAPPESILNPEYKVWKRQD 230

Query: 64  RLISSWLLGSMTENILEQVIHCKSSREIWMCLLQIFNSKNKAQVMRMKNRLQTIQKGGMS 123
           RLISSWLLGSM E+IL Q++HC S+++IW  L  I++S+  A+ M+ KN+L  ++KG +S
Sbjct: 231 RLISSWLLGSMGEDILNQMLHCTSAKQIWQTLQGIYSSRYLAKAMQFKNKLHNMKKGVIS 290

Query: 124 LSEYFSQIKKCIDALAAIGKTIDEEDHVLYILGGLGPDYESMVSAITATTEDQSVQEVMA 183
           L EYF +I++C+DALA+I K I  +DH+LYIL GLG +Y+SM+S I+A T+  SVQ+VM+
Sbjct: 291 LKEYFLKIQQCVDALASINKPISTDDHILYILAGLGNEYQSMISVISARTDSPSVQDVMS 350

Query: 184 LLLTHENQIERKLKS 188
           LLLT E+QIE K+ S
Sbjct: 351 LLLTQESQIESKITS 365

[Close]

BLAST of Lag0000336 vs. NCBI nr
Match: KAA0067213.1 (keratin, type II cytoskeletal 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 183.0 bits (463), Expect = 2.5e-42
Identity = 93/170 (54.71%), Postives = 122/170 (71.76%), Query Frame = 0


Query: 1   MWKVQIEFALEGHDLENFINSDVEPPPKRI--SVSENSSATKV-NPEYTKWKKQDRLISS 60
           +WK QI  ALE +DLENF+ S+ EPP K +  + S ++SAT+  NP Y  WK+QDRLISS
Sbjct: 41  LWKFQILTALEAYDLENFLESESEPPSKYLISTGSSSASATRTPNPTYKVWKRQDRLISS 100

Query: 61  WLLGSMTENILEQVIHCKSSREIWMCLLQIFNSKNKAQVMRMKNRLQTIQKGGMSLSEYF 120
           WLLGSM+E IL Q++HCKS++EIW  L  IF+S+  AQ M+ KN+L  I+K  M L EYF
Sbjct: 101 WLLGSMSEEILNQMLHCKSAKEIWGTLQGIFSSRYLAQAMKFKNKLHNIKKESMPLKEYF 160

Query: 121 SQIKKCIDALAAIGKTIDEEDHVLYILGGLGPDYESMVSAITATTEDQSV 168
            +I+  +DALA+I K +  +DH+LYIL GLG DY+SM+S I   TE  SV
Sbjct: 161 LKIQHRVDALASINKPVSSDDHILYILAGLGSDYQSMISVIFPRTESPSV 210

[Close]

BLAST of Lag0000336 vs. ExPASy Swiss-Prot
Match: Q94HW2 (Retrovirus-related Pol polyprotein from transposon RE1 OS=Arabidopsis thaliana OX=3702 GN=RE1 PE=2 SV=1)

HSP 1 Score: 102.8 bits (255), Expect = 4.3e-21
Identity = 52/181 (28.73%), Postives = 100/181 (55.25%), Query Frame = 0


Query: 1   MWKVQIEFALEGHDLENFINSDVEPPPKRISVSENSSATKVNPEYTKWKKQDRLISSWLL 60
           MW  Q+    +G++L  F++     PP  I      +A +VNP+YT+WK+QD+LI S +L
Sbjct: 33  MWSRQVHALFDGYELAGFLDGSTTMPPATIG---TDAAPRVNPDYTRWKRQDKLIYSAVL 92

Query: 61  GSMTENILEQVIHCKSSREIWMCLLQIFNSKNKAQVMRMKNRLQTIQKGGMSLSEYFSQI 120
           G+++ ++   V    ++ +IW  L +I+ + +   V +++ +L+   KG  ++ +Y   +
Sbjct: 93  GAISMSVQPAVSRATTAAQIWETLRKIYANPSYGHVTQLRTQLKQWTKGTKTIDDYMQGL 152

Query: 121 KKCIDALAAIGKTIDEEDHVLYILGGLGPDYESMVSAITATTEDQSVQEVMALLLTHENQ 180
               D LA +GK +D ++ V  +L  L  +Y+ ++  I A     ++ E+   LL HE++
Sbjct: 153 VTRFDQLALLGKPMDHDEQVERVLENLPEEYKPVIDQIAAKDTPPTLTEIHERLLNHESK 210

Query: 181 I 182
           I
Sbjct: 213 I 210

[Close]

BLAST of Lag0000336 vs. ExPASy Swiss-Prot
Match: Q9ZT94 (Retrovirus-related Pol polyprotein from transposon RE2 OS=Arabidopsis thaliana OX=3702 GN=RE2 PE=4 SV=1)

HSP 1 Score: 79.3 bits (194), Expect = 5.1e-14
Identity = 45/181 (24.86%), Postives = 91/181 (50.28%), Query Frame = 0


Query: 1   MWKVQIEFALEGHDLENFINSDVEPPPKRISVSENSSATKVNPEYTKWKKQDRLISSWLL 60
           MW  Q+    +G++L  F++     PP  I      +  +VNP+YT+W++QD+LI S +L
Sbjct: 33  MWSRQVHALFDGYELAGFLDGSTPMPPATIG---TDAVPRVNPDYTRWRRQDKLIYSAIL 92

Query: 61  GSMTENILEQVIHCKSSREIWMCLLQIFNSKNKAQVMRMKNRLQTIQKGGMSLSEYFSQI 120
           G+++ ++   V    ++ +IW  L +I+ + +   V +++               + ++ 
Sbjct: 93  GAISMSVQPAVSRATTAAQIWETLRKIYANPSYGHVTQLR---------------FITR- 152

Query: 121 KKCIDALAAIGKTIDEEDHVLYILGGLGPDYESMVSAITATTEDQSVQEVMALLLTHENQ 180
               D LA +GK +D ++ V  +L  L  DY+ ++  I A     S+ E+   L+  E++
Sbjct: 153 ---FDQLALLGKPMDHDEQVERVLENLPDDYKPVIDQIAAKDTPPSLTEIHERLINRESK 191

Query: 181 I 182
           +
Sbjct: 213 L 191

The following BLAST results are available for this feature:

BLAST of Lag0000336 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0048297.1	3.5e-52	57.37	Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Cucumis melo var. m...	[more]
TYK10642.1	3.5e-52	57.37	Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Cucumis melo var. m...	[more]
KAA0053143.1	1.1e-50	56.32	keratin, type II cytoskeletal 1-like [Cucumis melo var. makuwa]	[more]
KAA0045111.1	4.1e-45	50.26	putative glutathione S-transferase isoform X1 [Cucumis melo var. makuwa] >TYK236...	[more]
KAA0067213.1	2.5e-42	54.71	keratin, type II cytoskeletal 1-like [Cucumis melo var. makuwa]	[more]

BLAST of Lag0000336 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q94HW2	4.3e-21	28.73	Retrovirus-related Pol polyprotein from transposon RE1 OS=Arabidopsis thaliana O...	[more]
Q9ZT94	5.1e-14	24.86	Retrovirus-related Pol polyprotein from transposon RE2 OS=Arabidopsis thaliana O...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PFAM	PF14223	Retrotran_gag_2	coord: 48..180 e-value: 3.2E-25 score: 88.5
None	No IPR available	PANTHER	PTHR34222	FAMILY NOT NAMED	coord: 4..181
None	No IPR available	PANTHER	PTHR34222:SF48	SUBFAMILY NOT NAMED	coord: 4..181

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0000336.1	Lag0000336.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0034641	cellular nitrogen compound metabolic process
biological_process	GO:0071704	organic substance metabolic process
molecular_function	GO:0005488	binding
molecular_function	GO:0016740	transferase activity