Lag0000250 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0000250
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionCore-2/I-branching beta-1,6-N-acetylglucosaminyltransferase family protein
Locationchr4: 2301711 .. 2304119 (-)
RNA-Seq ExpressionLag0000250
SyntenyLag0000250
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGGTTTGAACAAAAGCAACCCCAATGGCCTCTCAAGTCCCCAAATCCCAATTTTCAACTCAAACATGTCTTCCATTTCCTCTTCTTCCTTGTTGGATTTTCTCTTGGAATCATCCTTTGCTTATACTTCAAAACCTTATTCTCTCTCACTTCACAACCTTCCTCCTCCATTCCAACCCCTAACTCTCCTTCTTCCTTCCCCACCTCACCACCGTCGTCCCTGCTCGCATCTACCTCTCCTCCACCACCACCACCAGATCGACCTCAGCCTCGGCCTCCGTCAGTTCTAGTTTTATCAATCCCAAGAAATGGGAGCTCCTCCAAGTCATCAAGTGTTTCACTTGAAGAACATAAGTCTTTGCTCCACAACATGAGTGATGAGGAGCTGTTTTGGAGAGCAAGCATGGTTCCTAGGATTGTGGAGCTGAGTGACAAGACAGTTCCAAAGGTGGCTTTCATGTTTTTGACGGCTGGACCTTTGCCACTGGCTCTTCTTTGGGAGAAGTTTTTTAAAGGCCACAATGGATTCTACTCCATTTATGTTCATTCACATCCCTCTTATGCTCATGAAATGCCTCAAAATTCTGTTTTTTATGGTACAAGGATACCTAGTCAGGTTGGTTTCTTCTTCTGTGTTCTTTTTATTTTTATTTTTTTGATATTTTTTTTTTAAATATTGACTATTTGGAATTTGAAAATTTTCGAAGGTTATTATATTTCTTTCTTCAAATATTATTATTAAGTTTACATTTGTTGAAATGAAATTAACTTTTATTATTATATATAATTTACACTAGGTGAGGACTTGAACTTTGAGAAAATTTCTAATACTAGGATAGTATGAATAATTTTCCATATATACTACCTTCATTTATTGTTTGCCTTGAACGAATCATTTAAGATGATATGGGATCCATGGAATAAAATAGGGAAAATTATCTTTTTGGTCCCTAAGTTTTGAGAAATATGTGCGTTTCGTTCCTGAGTTTTGAAATATAGGTTTAAAAGGTCCCTAAGTTTTCAAAATGGACCTTTTTAGTCCCTGAGTTTTGAGAAATAGGTTTAAAAGGTCCCTAACATTATTTTTTCCTCTTTTTTAAATTAATATTTTTTATTATTTTTAATATTGAATGGTTTTGTAAATTAAAAAAAATAAAAAGTATTAATTTAAAAAATAAATTATTATTTTTAATATTGAATGGTTTTGTAAATTAAATGATGTGTGAAATGCACAAGATTATTAGTGTTTGACTAATAAATTGGATTCAAAGAGGTTATTACGATTTGTTATTTATTACTTATTATTATTATTTTTAAATCTTAATCACATTTCTAAAACATATAACAAGAATTTAAAAATTACGGTCTTGTTTTGTTTTTAAAAATTAAGTTTCTTTGCTAATAATTTATTTGTTTATTTATTTTTATCTTTTTGTTAAAAAAAAAAATCTAGACAAAATTTTAAAAAGAGGTCAACTTCTAGGTTTTTTTTTCATTTCAAAATTCAGCTGACTATTGACTTTTCAGATTTAAAACAATAAAAGTTGTTCAGACAACCTCGAAATTTTTAAAAAATATAAGACAAAATTAAAGCGACTTCAACAAAAGGGTCTTCTTTTTATTATTATTAATTTTTTAAAGTTTTTCTAATTTGAGCTAAAATTCTATTAAGAAAAGAAACTTATTTAGAAAATAAATTATATGAAAACAACTTTGGTTAGGGGTTATGAAAGTAGCTGTTTTTAATAACTTTGATTTGCAGCCTGTGTATTGGGGAACTGCAACCATGATTGATGCAGAGAGACGCCTTCTAGCAAACGCCCTTTTAGACTTTTCAAACCAAAGATTTGTTCTTCTCTCTGACTCTTGCATCCCTTTGTTCAACTTCAACACCATCTACAATCATCTCATGAGCTCAAACCGAAGCTTTCTCGCTTCTTTCGACGACCCGAGAAAGTCCTGTCGGGGCCGCTACAACCCCCAAATGTCACCGGAGATAAACATCACCAACTGGAGAAAGGGTTCCCAATGGTTCGAGGTACATCGAGAGGTTGCTCTTAGCATTGTTTCCGACACGAAATACTATCCGATTTTTAAAAACTATTGCCTCCCACCTTGCTACATGGATGAGCATTACATACCAACTTTGGTGACTATGTTGCAGCCTGAATTGAATTCTAATAGGAGCATCACTTGGGTTGACTGGTCCAAGGGTGGCCCTCATCCAAGTAAATTTGGATGGAATGATATCACTGATGAGTTCTTAAATAGGATAAGGTTTGAATCAACATGTAATGAGGGTCAAAATTCCACTTCCATTTGTTTCCTTTTTGCTAGAAAATTTTTACCAAACACTTTGGAGCCTTTGTTGAGGGTCACTCCTCTATTGCTTTGTATTGATCCATAG

mRNA sequence

ATGGGGTTTGAACAAAAGCAACCCCAATGGCCTCTCAAGTCCCCAAATCCCAATTTTCAACTCAAACATGTCTTCCATTTCCTCTTCTTCCTTGTTGGATTTTCTCTTGGAATCATCCTTTGCTTATACTTCAAAACCTTATTCTCTCTCACTTCACAACCTTCCTCCTCCATTCCAACCCCTAACTCTCCTTCTTCCTTCCCCACCTCACCACCGTCGTCCCTGCTCGCATCTACCTCTCCTCCACCACCACCACCAGATCGACCTCAGCCTCGGCCTCCGTCAGTTCTAGTTTTATCAATCCCAAGAAATGGGAGCTCCTCCAAGTCATCAAGTGTTTCACTTGAAGAACATAAGTCTTTGCTCCACAACATGAGTGATGAGGAGCTGTTTTGGAGAGCAAGCATGGTTCCTAGGATTGTGGAGCTGAGTGACAAGACAGTTCCAAAGGTGGCTTTCATGTTTTTGACGGCTGGACCTTTGCCACTGGCTCTTCTTTGGGAGAAGTTTTTTAAAGGCCACAATGGATTCTACTCCATTTATGTTCATTCACATCCCTCTTATGCTCATGAAATGCCTCAAAATTCTGTTTTTTATGGTACAAGGATACCTAGTCAGCCTGTGTATTGGGGAACTGCAACCATGATTGATGCAGAGAGACGCCTTCTAGCAAACGCCCTTTTAGACTTTTCAAACCAAAGATTTGTTCTTCTCTCTGACTCTTGCATCCCTTTGTTCAACTTCAACACCATCTACAATCATCTCATGAGCTCAAACCGAAGCTTTCTCGCTTCTTTCGACGACCCGAGAAAGTCCTGTCGGGGCCGCTACAACCCCCAAATGTCACCGGAGATAAACATCACCAACTGGAGAAAGGGTTCCCAATGGTTCGAGGTACATCGAGAGGTTGCTCTTAGCATTGTTTCCGACACGAAATACTATCCGATTTTTAAAAACTATTGCCTCCCACCTTGCTACATGGATGAGCATTACATACCAACTTTGGTGACTATGTTGCAGCCTGAATTGAATTCTAATAGGAGCATCACTTGGGTTGACTGGTCCAAGGGTGGCCCTCATCCAAGTAAATTTGGATGGAATGATATCACTGATGAGTTCTTAAATAGGATAAGGTTTGAATCAACATGTAATGAGGGTCAAAATTCCACTTCCATTTGTTTCCTTTTTGCTAGAAAATTTTTACCAAACACTTTGGAGCCTTTGTTGAGGGTCACTCCTCTATTGCTTTGTATTGATCCATAG

Coding sequence (CDS)

ATGGGGTTTGAACAAAAGCAACCCCAATGGCCTCTCAAGTCCCCAAATCCCAATTTTCAACTCAAACATGTCTTCCATTTCCTCTTCTTCCTTGTTGGATTTTCTCTTGGAATCATCCTTTGCTTATACTTCAAAACCTTATTCTCTCTCACTTCACAACCTTCCTCCTCCATTCCAACCCCTAACTCTCCTTCTTCCTTCCCCACCTCACCACCGTCGTCCCTGCTCGCATCTACCTCTCCTCCACCACCACCACCAGATCGACCTCAGCCTCGGCCTCCGTCAGTTCTAGTTTTATCAATCCCAAGAAATGGGAGCTCCTCCAAGTCATCAAGTGTTTCACTTGAAGAACATAAGTCTTTGCTCCACAACATGAGTGATGAGGAGCTGTTTTGGAGAGCAAGCATGGTTCCTAGGATTGTGGAGCTGAGTGACAAGACAGTTCCAAAGGTGGCTTTCATGTTTTTGACGGCTGGACCTTTGCCACTGGCTCTTCTTTGGGAGAAGTTTTTTAAAGGCCACAATGGATTCTACTCCATTTATGTTCATTCACATCCCTCTTATGCTCATGAAATGCCTCAAAATTCTGTTTTTTATGGTACAAGGATACCTAGTCAGCCTGTGTATTGGGGAACTGCAACCATGATTGATGCAGAGAGACGCCTTCTAGCAAACGCCCTTTTAGACTTTTCAAACCAAAGATTTGTTCTTCTCTCTGACTCTTGCATCCCTTTGTTCAACTTCAACACCATCTACAATCATCTCATGAGCTCAAACCGAAGCTTTCTCGCTTCTTTCGACGACCCGAGAAAGTCCTGTCGGGGCCGCTACAACCCCCAAATGTCACCGGAGATAAACATCACCAACTGGAGAAAGGGTTCCCAATGGTTCGAGGTACATCGAGAGGTTGCTCTTAGCATTGTTTCCGACACGAAATACTATCCGATTTTTAAAAACTATTGCCTCCCACCTTGCTACATGGATGAGCATTACATACCAACTTTGGTGACTATGTTGCAGCCTGAATTGAATTCTAATAGGAGCATCACTTGGGTTGACTGGTCCAAGGGTGGCCCTCATCCAAGTAAATTTGGATGGAATGATATCACTGATGAGTTCTTAAATAGGATAAGGTTTGAATCAACATGTAATGAGGGTCAAAATTCCACTTCCATTTGTTTCCTTTTTGCTAGAAAATTTTTACCAAACACTTTGGAGCCTTTGTTGAGGGTCACTCCTCTATTGCTTTGTATTGATCCATAG

Protein sequence

MGFEQKQPQWPLKSPNPNFQLKHVFHFLFFLVGFSLGIILCLYFKTLFSLTSQPSSSIPTPNSPSSFPTSPPSSLLASTSPPPPPPDRPQPRPPSVLVLSIPRNGSSSKSSSVSLEEHKSLLHNMSDEELFWRASMVPRIVELSDKTVPKVAFMFLTAGPLPLALLWEKFFKGHNGFYSIYVHSHPSYAHEMPQNSVFYGTRIPSQPVYWGTATMIDAERRLLANALLDFSNQRFVLLSDSCIPLFNFNTIYNHLMSSNRSFLASFDDPRKSCRGRYNPQMSPEINITNWRKGSQWFEVHREVALSIVSDTKYYPIFKNYCLPPCYMDEHYIPTLVTMLQPELNSNRSITWVDWSKGGPHPSKFGWNDITDEFLNRIRFESTCNEGQNSTSICFLFARKFLPNTLEPLLRVTPLLLCIDP
Homology
BLAST of Lag0000250 vs. NCBI nr
Match: XP_038887114.1 (glycosyltransferase BC10-like [Benincasa hispida])

HSP 1 Score: 685.3 bits (1767), Expect = 3.4e-193
Identity = 360/436 (82.57%), Postives = 378/436 (86.70%), Query Frame = 0

Query: 1   MGFEQKQPQWPLKSPNPNFQLKHVFHFLFFLVGFSLGIILCLYFKTLFSLTSQPSSSIPT 60
           MGFEQKQPQ  LKS N NFQLKHVFHFLFFLVGFS GIILCLYFKT +SLT+      PT
Sbjct: 1   MGFEQKQPQGFLKSHNLNFQLKHVFHFLFFLVGFSFGIILCLYFKT-YSLTN------PT 60

Query: 61  -PNSPSSFPTSPPSSLLAS------TSPPPPPPDRPQPRPPSVLVLSIPRNGSSSKSSS- 120
            PNSPSSF   PPSS L +       SPPP PPD+ QPR   +L LSIPRNGSSS +SS 
Sbjct: 61  PPNSPSSFSALPPSSFLLAPVSSPPLSPPPSPPDQSQPRSRPLLFLSIPRNGSSSSNSSR 120

Query: 121 --VSLEEHKSLLHNMSDEELFWRASMVPRIVELSDKTVP-KVAFMFLTAGPLPLALLWEK 180
             VSLEEHKSL+HNMSDEELFWRASMVPRIVE SDKTVP KVAFMFLT GPLPLA+LWEK
Sbjct: 121 VIVSLEEHKSLVHNMSDEELFWRASMVPRIVE-SDKTVPKKVAFMFLTTGPLPLAILWEK 180

Query: 181 FFKGHNGFYSIYVHSHPSYAHEMPQNSVFYGTRIPSQPVYWGTATMIDAERRLLANALLD 240
           FFKGHNG YSIYVHSHPSYA EMPQ SVFYG RIPSQ VYWGTA+MIDAERRLLANALLD
Sbjct: 181 FFKGHNGLYSIYVHSHPSYADEMPQTSVFYGRRIPSQAVYWGTASMIDAERRLLANALLD 240

Query: 241 FSNQRFVLLSDSCIPLFNFNTIYNHLMSSNRSFLASFDDPRKSCRGRYNPQMSPEINITN 300
            SNQRFVLLSDSCIPLFNF TIYNH+MSSN SFL SF DPRKSC+GRYNPQMSPEINIT+
Sbjct: 241 LSNQRFVLLSDSCIPLFNFTTIYNHVMSSNLSFLGSFYDPRKSCQGRYNPQMSPEINITD 300

Query: 301 WRKGSQWFEVHREVALSIVSDTKYYPIFKNYCLPPCYMDEHYIPTLVTMLQPELNSNRSI 360
           WRKGSQWFEVHRE+AL IVSDTKYYPIFKNYCLPPCYMDEHYIPTLV ML+PELNSNRSI
Sbjct: 301 WRKGSQWFEVHRELALHIVSDTKYYPIFKNYCLPPCYMDEHYIPTLVHMLKPELNSNRSI 360

Query: 361 TWVDWSKGGPHPSKFGWNDITDEFLNRIRFESTCN-----EGQNSTSICFLFARKFLPNT 420
           TWVDWSKGGPHPSKFGWNDITDEFLNRIRFESTCN     +  +++SICFLFARKFLPNT
Sbjct: 361 TWVDWSKGGPHPSKFGWNDITDEFLNRIRFESTCNNENYDQYYSTSSICFLFARKFLPNT 420

BLAST of Lag0000250 vs. NCBI nr
Match: KAA0048474.1 (Core-2/I-branching beta-1,6-N-acetylglucosaminyltransferase family protein [Cucumis melo var. makuwa] >TYK28270.1 Core-2/I-branching beta-1,6-N-acetylglucosaminyltransferase family protein [Cucumis melo var. makuwa])

HSP 1 Score: 660.6 bits (1703), Expect = 9.0e-186
Identity = 345/432 (79.86%), Postives = 371/432 (85.88%), Query Frame = 0

Query: 1   MGFEQKQPQWPLKSPNPNFQLKHVFHFLFFLVGFSLGIILCLYFKTLFSLTSQPSSSIPT 60
           MGFEQKQ Q   KS N +FQLKHVFHFLFFLVGFSLGIILCLY K+ F LT+      PT
Sbjct: 1   MGFEQKQSQ---KSHNLHFQLKHVFHFLFFLVGFSLGIILCLYSKSSFFLTN------PT 60

Query: 61  PNSPSSFPTSPPSSLLASTSPPPP---PPDRPQPRPPSVLVLSIPR-NGSSSKSSS---V 120
           PNS SSF   PPSSL  + SPPPP   P D+PQ     +LVL+IPR NG+SS +SS   V
Sbjct: 61  PNSSSSFSAPPPSSLFETVSPPPPLPLPDDQPQSPSRPLLVLTIPRSNGTSSSNSSKVVV 120

Query: 121 SLEEHKSLLHNMSDEELFWRASMVPRIVELSDKTVP-KVAFMFLTAGPLPLALLWEKFFK 180
           SLEEHKSL+HNMSDEELFWRASMVPRIVE + KTVP KVAFMFLTAGPLPLA LWEKFF+
Sbjct: 121 SLEEHKSLVHNMSDEELFWRASMVPRIVEGNYKTVPKKVAFMFLTAGPLPLATLWEKFFE 180

Query: 181 GHNGFYSIYVHSHPSYAHEMPQNSVFYGTRIPSQPVYWGTATMIDAERRLLANALLDFSN 240
           GHNG YSIYVHSHPSY  E+PQ SVFYG RIPSQ VYWGTA+MIDAERRLLANALLD SN
Sbjct: 181 GHNGLYSIYVHSHPSYVDEIPQTSVFYGRRIPSQAVYWGTASMIDAERRLLANALLDLSN 240

Query: 241 QRFVLLSDSCIPLFNFNTIYNHLMSSNRSFLASFDDPRKSCRGRYNPQMSPEINITNWRK 300
           QRFVLLSDSCIPLFNFNTIYNHLM+SN SF++SF DPRKSC GRYNPQMSPEINITNWRK
Sbjct: 241 QRFVLLSDSCIPLFNFNTIYNHLMTSNLSFISSFYDPRKSCGGRYNPQMSPEINITNWRK 300

Query: 301 GSQWFEVHREVALSIVSDTKYYPIFKNYCLPPCYMDEHYIPTLVTMLQPELNSNRSITWV 360
           GSQWFEVHRE+AL IVSDTKYYPIFK YCLPPCYMDEHYIPTLV +LQPELNSNRSITWV
Sbjct: 301 GSQWFEVHRELALRIVSDTKYYPIFKKYCLPPCYMDEHYIPTLVHLLQPELNSNRSITWV 360

Query: 361 DWSKGGPHPSKFGWNDITDEFLNRIRFESTCN----EGQNSTSICFLFARKFLPNTLEPL 420
           DWS+GGPHPSKFGWNDI DEFLN+IRFESTC+    +  +++SICFLFARKFLPNTLEPL
Sbjct: 361 DWSRGGPHPSKFGWNDIGDEFLNKIRFESTCDNENYQNYSTSSICFLFARKFLPNTLEPL 420

BLAST of Lag0000250 vs. NCBI nr
Match: XP_008461555.1 (PREDICTED: uncharacterized protein LOC103500127 [Cucumis melo])

HSP 1 Score: 659.4 bits (1700), Expect = 2.0e-185
Identity = 345/432 (79.86%), Postives = 370/432 (85.65%), Query Frame = 0

Query: 1   MGFEQKQPQWPLKSPNPNFQLKHVFHFLFFLVGFSLGIILCLYFKTLFSLTSQPSSSIPT 60
           MGFEQKQ Q   KS N +FQLKHVFHFLFFLVGFSLGIILCLY K+ F LT+      PT
Sbjct: 1   MGFEQKQSQ---KSHNLHFQLKHVFHFLFFLVGFSLGIILCLYSKSSFFLTN------PT 60

Query: 61  PNSPSSFPTSPPSSLLASTSPPPP---PPDRPQPRPPSVLVLSIPR-NGSSSKSSS---V 120
           PNS SSF   PPSSL    SPPPP   P D+PQ     +LVL+IPR NG+SS +SS   V
Sbjct: 61  PNSSSSFSAPPPSSLFEIVSPPPPLPLPDDQPQSPSRPLLVLTIPRSNGTSSSNSSKVVV 120

Query: 121 SLEEHKSLLHNMSDEELFWRASMVPRIVELSDKTVP-KVAFMFLTAGPLPLALLWEKFFK 180
           SLEEHKSL+HNMSDEELFWRASMVPRIVE + KTVP KVAFMFLTAGPLPLA LWEKFF+
Sbjct: 121 SLEEHKSLVHNMSDEELFWRASMVPRIVEGNYKTVPKKVAFMFLTAGPLPLATLWEKFFE 180

Query: 181 GHNGFYSIYVHSHPSYAHEMPQNSVFYGTRIPSQPVYWGTATMIDAERRLLANALLDFSN 240
           GHNG YSIYVHSHPSY  E+PQ SVFYG RIPSQ VYWGTA+MIDAERRLLANALLD SN
Sbjct: 181 GHNGLYSIYVHSHPSYVDEIPQTSVFYGRRIPSQAVYWGTASMIDAERRLLANALLDLSN 240

Query: 241 QRFVLLSDSCIPLFNFNTIYNHLMSSNRSFLASFDDPRKSCRGRYNPQMSPEINITNWRK 300
           QRFVLLSDSCIPLFNFNTIYNHLM+SN SF++SF DPRKSC GRYNPQMSPEINITNWRK
Sbjct: 241 QRFVLLSDSCIPLFNFNTIYNHLMTSNLSFISSFYDPRKSCGGRYNPQMSPEINITNWRK 300

Query: 301 GSQWFEVHREVALSIVSDTKYYPIFKNYCLPPCYMDEHYIPTLVTMLQPELNSNRSITWV 360
           GSQWFEVHRE+AL IVSDTKYYPIFK YCLPPCYMDEHYIPTLV +LQPELNSNRSITWV
Sbjct: 301 GSQWFEVHRELALRIVSDTKYYPIFKKYCLPPCYMDEHYIPTLVHLLQPELNSNRSITWV 360

Query: 361 DWSKGGPHPSKFGWNDITDEFLNRIRFESTCN----EGQNSTSICFLFARKFLPNTLEPL 420
           DWS+GGPHPSKFGWNDI DEFLN+IRFESTC+    +  +++SICFLFARKFLPNTLEPL
Sbjct: 361 DWSRGGPHPSKFGWNDIGDEFLNKIRFESTCDNENYQNYSTSSICFLFARKFLPNTLEPL 420

BLAST of Lag0000250 vs. NCBI nr
Match: XP_011651351.1 (glycosyltransferase BC10 [Cucumis sativus] >KGN57704.1 hypothetical protein Csa_010749 [Cucumis sativus])

HSP 1 Score: 650.2 bits (1676), Expect = 1.2e-182
Identity = 336/433 (77.60%), Postives = 364/433 (84.06%), Query Frame = 0

Query: 1   MGFEQKQPQWPLKSPNPNFQLKHVFHFLFFLVGFSLGIILCLYFKTLFSLTSQPSSSIPT 60
           MGFEQKQ Q   KS N +FQLKHVFHFLFFL+GFSLGIILCLYFK+ F L +      PT
Sbjct: 1   MGFEQKQSQ---KSHNLHFQLKHVFHFLFFLIGFSLGIILCLYFKSSFFLIN------PT 60

Query: 61  PNSPSSFPTSPPSSLLASTSPPPP---PPDRPQPRPPSVLVLSIPRNGSSSKSSS----V 120
           PNS SSF   PPSSL  + S PPP   P D+P P P  +LVLS PRN  SS S+S    V
Sbjct: 61  PNSSSSFSAPPPSSLFETVSSPPPQPLPDDQPHPHPHPLLVLSNPRNNGSSSSNSSKLVV 120

Query: 121 SLEEHKSLLHNMSDEELFWRASMVPRIVELSDKTVP-KVAFMFLTAGPLPLALLWEKFFK 180
           SLEEHKSL+HNM+DEELFW ASMVPRIVE + KTVP KVAFMFLT+GPLPLA LWEKFF+
Sbjct: 121 SLEEHKSLVHNMNDEELFWTASMVPRIVESNYKTVPKKVAFMFLTSGPLPLATLWEKFFE 180

Query: 181 GHNGFYSIYVHSHPSYAHEMPQNSVFYGTRIPSQPVYWGTATMIDAERRLLANALLDFSN 240
           G+NG YSIYVHSHPSY  E+PQ SVFYG RIPSQ VYWGTA+MIDAERRLLANALLD SN
Sbjct: 181 GNNGLYSIYVHSHPSYVDEIPQTSVFYGRRIPSQAVYWGTASMIDAERRLLANALLDLSN 240

Query: 241 QRFVLLSDSCIPLFNFNTIYNHLMSSNRSFLASFDDPRKSCRGRYNPQMSPEINITNWRK 300
            RFVLLSDSCIPLFNFNTIYNHL++S  SF++SF DPRKSC GRYNPQMSP+INITNWRK
Sbjct: 241 HRFVLLSDSCIPLFNFNTIYNHLITSKLSFISSFYDPRKSCGGRYNPQMSPQINITNWRK 300

Query: 301 GSQWFEVHREVALSIVSDTKYYPIFKNYCLPPCYMDEHYIPTLVTMLQPELNSNRSITWV 360
           GSQWFEVHRE+AL IVSDTKYYPIFKNYCLPPCYMDEHYIPTLV MLQPELNSNRSITWV
Sbjct: 301 GSQWFEVHRELALRIVSDTKYYPIFKNYCLPPCYMDEHYIPTLVHMLQPELNSNRSITWV 360

Query: 361 DWSKGGPHPSKFGWNDITDEFLNRIRFESTCN-----EGQNSTSICFLFARKFLPNTLEP 420
           DWS+GGPHPSKFGW DI DEFLN+IRFESTCN     +  +++SICFLFARKFLPNTLEP
Sbjct: 361 DWSRGGPHPSKFGWKDIGDEFLNKIRFESTCNNETYDQNYSTSSICFLFARKFLPNTLEP 420

BLAST of Lag0000250 vs. NCBI nr
Match: KAG7017896.1 (hypothetical protein SDJN02_19762, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 638.3 bits (1645), Expect = 4.8e-179
Identity = 332/427 (77.75%), Postives = 357/427 (83.61%), Query Frame = 0

Query: 1   MGFEQKQPQWPLKSPNPNFQLKHVFHFLFFLVGFSLGIILCLYFKTLFSLTSQPSSSIPT 60
           MGF+QK    P    NP+F LKHVF FLFFLVGFSLGIILC Y KT FSL +  + + P 
Sbjct: 1   MGFDQK--PLPKSHINPHFHLKHVFQFLFFLVGFSLGIILCFYVKT-FSLVNPTTPNPPP 60

Query: 61  PNSPSSFPTSPPSSLLAS----TSPPPPPPDRPQPRPPSVLVLSIPRNGSSSKSSS--VS 120
           P  P SFP  PPSSLLA      SPPP P       PPS+LVLSIPRNGSSS++SS  VS
Sbjct: 61  P--PPSFPAPPPSSLLAPPIVVLSPPPTP-------PPSILVLSIPRNGSSSEASSSVVS 120

Query: 121 LEEHKSLLHNMSDEELFWRASMVPRIVELSDKTVP-KVAFMFLTAGPLPLALLWEKFFKG 180
           LEEHKSLLHNMSDEELFWR+SMVPR++E+SDKT P KVAFMFLTAGPLPLA LWE FF+G
Sbjct: 121 LEEHKSLLHNMSDEELFWRSSMVPRMLEVSDKTAPRKVAFMFLTAGPLPLADLWEMFFEG 180

Query: 181 HNGFYSIYVHSHPSYAHEMPQNSVFYGTRIPSQPVYWGTATMIDAERRLLANALLDFSNQ 240
           H+GFYSIYVHSHPSY HEMP  SVFYG RIPSQPV+WGTA+M+DAERRLLANALLD +NQ
Sbjct: 181 HHGFYSIYVHSHPSYVHEMPPTSVFYGRRIPSQPVHWGTASMVDAERRLLANALLDITNQ 240

Query: 241 RFVLLSDSCIPLFNFNTIYNHLMSSNRSFLASFDDPRKSCRGRYNPQMSPEINITNWRKG 300
           RFVLLSDSCIPLFNF TIYNHL++SN SF+ SFDDPRK  RGRYNP+M P INITNWRKG
Sbjct: 241 RFVLLSDSCIPLFNFTTIYNHLITSNLSFIGSFDDPRKLGRGRYNPKMWPMINITNWRKG 300

Query: 301 SQWFEVHREVALSIVSDTKYYPIFKNYCLPPCYMDEHYIPTLVTMLQPELNSNRSITWVD 360
           SQWFEVHRE+AL IVSDTKYYPIFKNYCLPPCYMDEHYIPTLV M+QPE N NRSITWVD
Sbjct: 301 SQWFEVHRELALGIVSDTKYYPIFKNYCLPPCYMDEHYIPTLVNMVQPERNHNRSITWVD 360

Query: 361 WSKGGPHPSKFGWNDITDEFLNRIRFESTCNEGQNSTSICFLFARKFLPNTLEPLLRVTP 420
           WSKGGPHP KFGWN I DEFLN IRFESTC    NS+SICFLFARKFLPNTLEPLLRVTP
Sbjct: 361 WSKGGPHPGKFGWNHINDEFLNTIRFESTC--ANNSSSICFLFARKFLPNTLEPLLRVTP 413

BLAST of Lag0000250 vs. ExPASy Swiss-Prot
Match: Q65XS5 (Glycosyltransferase BC10 OS=Oryza sativa subsp. japonica OX=39947 GN=BC10 PE=1 SV=1)

HSP 1 Score: 176.8 bits (447), Expect = 5.2e-43
Identity = 115/297 (38.72%), Postives = 162/297 (54.55%), Query Frame = 0

Query: 150 KVAFMFLTAGPLPLALLWEKFFKG-HNGFYSIYVHSHPSY--AHEMPQNSVFYGTRI-PS 209
           ++AF+F+    LPL L+W+ FF+G   G +SI+VHS P +       ++  FY  ++  S
Sbjct: 85  RLAFLFIARNRLPLDLVWDAFFRGDKEGRFSIFVHSRPGFVLTRATTRSGFFYNRQVNNS 144

Query: 210 QPVYWGTATMIDAERRLLANALLDFSNQRFVLLSDSCIPLFNFNTIYNHLMSSNRSFLAS 269
             V WG A+MI+AER LLA+AL D  N+RFV +SDSC+PL+NFN  Y+++MSS+ SF+ S
Sbjct: 145 VQVDWGEASMIEAERVLLAHALKDPLNERFVFVSDSCVPLYNFNYTYDYIMSSSTSFVDS 204

Query: 270 FDDPRKSCRGRYNPQMSPEINITNWRKGSQWFEVHREVALSIVSDTKYYPIFKNYC---- 329
           F D +    GRYNP+M P I + NWRKGSQW  + R+ A  +V D +  P F+ +C    
Sbjct: 205 FADTK---AGRYNPRMDPIIPVENWRKGSQWAVLTRKHAEVVVEDEEVLPEFQKHCRRRP 264

Query: 330 LPP------------------CYMDEHYIPTLVTM--LQPELNSNRSITWVDW------- 389
           LP                   C  DEHY+ TL+    L+ EL + RS+T   W       
Sbjct: 265 LPEFWRDWDRPIPAEAWKAHNCIPDEHYVQTLLAQHGLEEEL-TRRSVTHSAWDLSSSKD 324

Query: 390 -SKGGPHPSKFGWNDITDEFLNRIR-----FESTCNEGQNSTS-----ICFLFARKF 401
             + G HP  +  +D T   +  I+     +  T N  +  TS      CFLFARKF
Sbjct: 325 RERRGWHPVTYKISDATPALVKSIKDIDNIYYETENRKEWCTSNGKPAPCFLFARKF 377

BLAST of Lag0000250 vs. ExPASy TrEMBL
Match: A0A5D3DYE7 (Core-2/I-branching beta-1,6-N-acetylglucosaminyltransferase family protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold600G00780 PE=4 SV=1)

HSP 1 Score: 660.6 bits (1703), Expect = 4.4e-186
Identity = 345/432 (79.86%), Postives = 371/432 (85.88%), Query Frame = 0

Query: 1   MGFEQKQPQWPLKSPNPNFQLKHVFHFLFFLVGFSLGIILCLYFKTLFSLTSQPSSSIPT 60
           MGFEQKQ Q   KS N +FQLKHVFHFLFFLVGFSLGIILCLY K+ F LT+      PT
Sbjct: 1   MGFEQKQSQ---KSHNLHFQLKHVFHFLFFLVGFSLGIILCLYSKSSFFLTN------PT 60

Query: 61  PNSPSSFPTSPPSSLLASTSPPPP---PPDRPQPRPPSVLVLSIPR-NGSSSKSSS---V 120
           PNS SSF   PPSSL  + SPPPP   P D+PQ     +LVL+IPR NG+SS +SS   V
Sbjct: 61  PNSSSSFSAPPPSSLFETVSPPPPLPLPDDQPQSPSRPLLVLTIPRSNGTSSSNSSKVVV 120

Query: 121 SLEEHKSLLHNMSDEELFWRASMVPRIVELSDKTVP-KVAFMFLTAGPLPLALLWEKFFK 180
           SLEEHKSL+HNMSDEELFWRASMVPRIVE + KTVP KVAFMFLTAGPLPLA LWEKFF+
Sbjct: 121 SLEEHKSLVHNMSDEELFWRASMVPRIVEGNYKTVPKKVAFMFLTAGPLPLATLWEKFFE 180

Query: 181 GHNGFYSIYVHSHPSYAHEMPQNSVFYGTRIPSQPVYWGTATMIDAERRLLANALLDFSN 240
           GHNG YSIYVHSHPSY  E+PQ SVFYG RIPSQ VYWGTA+MIDAERRLLANALLD SN
Sbjct: 181 GHNGLYSIYVHSHPSYVDEIPQTSVFYGRRIPSQAVYWGTASMIDAERRLLANALLDLSN 240

Query: 241 QRFVLLSDSCIPLFNFNTIYNHLMSSNRSFLASFDDPRKSCRGRYNPQMSPEINITNWRK 300
           QRFVLLSDSCIPLFNFNTIYNHLM+SN SF++SF DPRKSC GRYNPQMSPEINITNWRK
Sbjct: 241 QRFVLLSDSCIPLFNFNTIYNHLMTSNLSFISSFYDPRKSCGGRYNPQMSPEINITNWRK 300

Query: 301 GSQWFEVHREVALSIVSDTKYYPIFKNYCLPPCYMDEHYIPTLVTMLQPELNSNRSITWV 360
           GSQWFEVHRE+AL IVSDTKYYPIFK YCLPPCYMDEHYIPTLV +LQPELNSNRSITWV
Sbjct: 301 GSQWFEVHRELALRIVSDTKYYPIFKKYCLPPCYMDEHYIPTLVHLLQPELNSNRSITWV 360

Query: 361 DWSKGGPHPSKFGWNDITDEFLNRIRFESTCN----EGQNSTSICFLFARKFLPNTLEPL 420
           DWS+GGPHPSKFGWNDI DEFLN+IRFESTC+    +  +++SICFLFARKFLPNTLEPL
Sbjct: 361 DWSRGGPHPSKFGWNDIGDEFLNKIRFESTCDNENYQNYSTSSICFLFARKFLPNTLEPL 420

BLAST of Lag0000250 vs. ExPASy TrEMBL
Match: A0A1S3CF00 (uncharacterized protein LOC103500127 OS=Cucumis melo OX=3656 GN=LOC103500127 PE=4 SV=1)

HSP 1 Score: 659.4 bits (1700), Expect = 9.7e-186
Identity = 345/432 (79.86%), Postives = 370/432 (85.65%), Query Frame = 0

Query: 1   MGFEQKQPQWPLKSPNPNFQLKHVFHFLFFLVGFSLGIILCLYFKTLFSLTSQPSSSIPT 60
           MGFEQKQ Q   KS N +FQLKHVFHFLFFLVGFSLGIILCLY K+ F LT+      PT
Sbjct: 1   MGFEQKQSQ---KSHNLHFQLKHVFHFLFFLVGFSLGIILCLYSKSSFFLTN------PT 60

Query: 61  PNSPSSFPTSPPSSLLASTSPPPP---PPDRPQPRPPSVLVLSIPR-NGSSSKSSS---V 120
           PNS SSF   PPSSL    SPPPP   P D+PQ     +LVL+IPR NG+SS +SS   V
Sbjct: 61  PNSSSSFSAPPPSSLFEIVSPPPPLPLPDDQPQSPSRPLLVLTIPRSNGTSSSNSSKVVV 120

Query: 121 SLEEHKSLLHNMSDEELFWRASMVPRIVELSDKTVP-KVAFMFLTAGPLPLALLWEKFFK 180
           SLEEHKSL+HNMSDEELFWRASMVPRIVE + KTVP KVAFMFLTAGPLPLA LWEKFF+
Sbjct: 121 SLEEHKSLVHNMSDEELFWRASMVPRIVEGNYKTVPKKVAFMFLTAGPLPLATLWEKFFE 180

Query: 181 GHNGFYSIYVHSHPSYAHEMPQNSVFYGTRIPSQPVYWGTATMIDAERRLLANALLDFSN 240
           GHNG YSIYVHSHPSY  E+PQ SVFYG RIPSQ VYWGTA+MIDAERRLLANALLD SN
Sbjct: 181 GHNGLYSIYVHSHPSYVDEIPQTSVFYGRRIPSQAVYWGTASMIDAERRLLANALLDLSN 240

Query: 241 QRFVLLSDSCIPLFNFNTIYNHLMSSNRSFLASFDDPRKSCRGRYNPQMSPEINITNWRK 300
           QRFVLLSDSCIPLFNFNTIYNHLM+SN SF++SF DPRKSC GRYNPQMSPEINITNWRK
Sbjct: 241 QRFVLLSDSCIPLFNFNTIYNHLMTSNLSFISSFYDPRKSCGGRYNPQMSPEINITNWRK 300

Query: 301 GSQWFEVHREVALSIVSDTKYYPIFKNYCLPPCYMDEHYIPTLVTMLQPELNSNRSITWV 360
           GSQWFEVHRE+AL IVSDTKYYPIFK YCLPPCYMDEHYIPTLV +LQPELNSNRSITWV
Sbjct: 301 GSQWFEVHRELALRIVSDTKYYPIFKKYCLPPCYMDEHYIPTLVHLLQPELNSNRSITWV 360

Query: 361 DWSKGGPHPSKFGWNDITDEFLNRIRFESTCN----EGQNSTSICFLFARKFLPNTLEPL 420
           DWS+GGPHPSKFGWNDI DEFLN+IRFESTC+    +  +++SICFLFARKFLPNTLEPL
Sbjct: 361 DWSRGGPHPSKFGWNDIGDEFLNKIRFESTCDNENYQNYSTSSICFLFARKFLPNTLEPL 420

BLAST of Lag0000250 vs. ExPASy TrEMBL
Match: A0A0A0L9P1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G257090 PE=4 SV=1)

HSP 1 Score: 650.2 bits (1676), Expect = 5.9e-183
Identity = 336/433 (77.60%), Postives = 364/433 (84.06%), Query Frame = 0

Query: 1   MGFEQKQPQWPLKSPNPNFQLKHVFHFLFFLVGFSLGIILCLYFKTLFSLTSQPSSSIPT 60
           MGFEQKQ Q   KS N +FQLKHVFHFLFFL+GFSLGIILCLYFK+ F L +      PT
Sbjct: 1   MGFEQKQSQ---KSHNLHFQLKHVFHFLFFLIGFSLGIILCLYFKSSFFLIN------PT 60

Query: 61  PNSPSSFPTSPPSSLLASTSPPPP---PPDRPQPRPPSVLVLSIPRNGSSSKSSS----V 120
           PNS SSF   PPSSL  + S PPP   P D+P P P  +LVLS PRN  SS S+S    V
Sbjct: 61  PNSSSSFSAPPPSSLFETVSSPPPQPLPDDQPHPHPHPLLVLSNPRNNGSSSSNSSKLVV 120

Query: 121 SLEEHKSLLHNMSDEELFWRASMVPRIVELSDKTVP-KVAFMFLTAGPLPLALLWEKFFK 180
           SLEEHKSL+HNM+DEELFW ASMVPRIVE + KTVP KVAFMFLT+GPLPLA LWEKFF+
Sbjct: 121 SLEEHKSLVHNMNDEELFWTASMVPRIVESNYKTVPKKVAFMFLTSGPLPLATLWEKFFE 180

Query: 181 GHNGFYSIYVHSHPSYAHEMPQNSVFYGTRIPSQPVYWGTATMIDAERRLLANALLDFSN 240
           G+NG YSIYVHSHPSY  E+PQ SVFYG RIPSQ VYWGTA+MIDAERRLLANALLD SN
Sbjct: 181 GNNGLYSIYVHSHPSYVDEIPQTSVFYGRRIPSQAVYWGTASMIDAERRLLANALLDLSN 240

Query: 241 QRFVLLSDSCIPLFNFNTIYNHLMSSNRSFLASFDDPRKSCRGRYNPQMSPEINITNWRK 300
            RFVLLSDSCIPLFNFNTIYNHL++S  SF++SF DPRKSC GRYNPQMSP+INITNWRK
Sbjct: 241 HRFVLLSDSCIPLFNFNTIYNHLITSKLSFISSFYDPRKSCGGRYNPQMSPQINITNWRK 300

Query: 301 GSQWFEVHREVALSIVSDTKYYPIFKNYCLPPCYMDEHYIPTLVTMLQPELNSNRSITWV 360
           GSQWFEVHRE+AL IVSDTKYYPIFKNYCLPPCYMDEHYIPTLV MLQPELNSNRSITWV
Sbjct: 301 GSQWFEVHRELALRIVSDTKYYPIFKNYCLPPCYMDEHYIPTLVHMLQPELNSNRSITWV 360

Query: 361 DWSKGGPHPSKFGWNDITDEFLNRIRFESTCN-----EGQNSTSICFLFARKFLPNTLEP 420
           DWS+GGPHPSKFGW DI DEFLN+IRFESTCN     +  +++SICFLFARKFLPNTLEP
Sbjct: 361 DWSRGGPHPSKFGWKDIGDEFLNKIRFESTCNNETYDQNYSTSSICFLFARKFLPNTLEP 420

BLAST of Lag0000250 vs. ExPASy TrEMBL
Match: A0A6J1F2L4 (uncharacterized protein LOC111441621 OS=Cucurbita moschata OX=3662 GN=LOC111441621 PE=4 SV=1)

HSP 1 Score: 634.8 bits (1636), Expect = 2.6e-178
Identity = 331/427 (77.52%), Postives = 357/427 (83.61%), Query Frame = 0

Query: 1   MGFEQKQPQWPLKSPNPNFQLKHVFHFLFFLVGFSLGIILCLYFKTLFSLTSQPSSSIPT 60
           MGF+QK    P    NP+F LKHVF FLFFLVGFSLGIILC Y KT FSL +  + + P 
Sbjct: 1   MGFDQK--PLPKSHINPHFHLKHVFQFLFFLVGFSLGIILCFYVKT-FSLVNPTTPNPPL 60

Query: 61  PNSPSSFPTSPPSSLLAS----TSPPPPPPDRPQPRPPSVLVLSIPRNGSSSKSSS--VS 120
           P  P SFP  P SSLLA      SPPP P       PPS+LVLSIPRNGSSS++SS  VS
Sbjct: 61  P--PPSFPAPPLSSLLAPPIVVLSPPPTP-------PPSILVLSIPRNGSSSEASSSVVS 120

Query: 121 LEEHKSLLHNMSDEELFWRASMVPRIVELSDKTVP-KVAFMFLTAGPLPLALLWEKFFKG 180
           LEEHKSLLHNMSDEELFWR+SMVPR++E+SDKT P KVAFMFLTAGPLPLA LWE FF+G
Sbjct: 121 LEEHKSLLHNMSDEELFWRSSMVPRMLEVSDKTAPRKVAFMFLTAGPLPLADLWEMFFEG 180

Query: 181 HNGFYSIYVHSHPSYAHEMPQNSVFYGTRIPSQPVYWGTATMIDAERRLLANALLDFSNQ 240
           H+GFYSIYVHSHPSY HEMP  SVFYG RIPSQPV+WGTA+M+DAERRLLANALLD +NQ
Sbjct: 181 HHGFYSIYVHSHPSYVHEMPPTSVFYGRRIPSQPVHWGTASMVDAERRLLANALLDITNQ 240

Query: 241 RFVLLSDSCIPLFNFNTIYNHLMSSNRSFLASFDDPRKSCRGRYNPQMSPEINITNWRKG 300
           RFVLLSDSCIPLFNF TIYNHL++SN SF++SFDDPRK  RGRYNP+M P INITNWRKG
Sbjct: 241 RFVLLSDSCIPLFNFTTIYNHLITSNLSFISSFDDPRKLGRGRYNPKMWPMINITNWRKG 300

Query: 301 SQWFEVHREVALSIVSDTKYYPIFKNYCLPPCYMDEHYIPTLVTMLQPELNSNRSITWVD 360
           SQWFEVHRE+AL IVSDTKYYPIFKNYCLPPCYMDEHYIPTLV M+QPE N NRSITWVD
Sbjct: 301 SQWFEVHRELALGIVSDTKYYPIFKNYCLPPCYMDEHYIPTLVNMVQPERNHNRSITWVD 360

Query: 361 WSKGGPHPSKFGWNDITDEFLNRIRFESTCNEGQNSTSICFLFARKFLPNTLEPLLRVTP 420
           WSKGGPHP KFGWN I DEFLN IRFESTC    NS+SICFLFARKFLPNTLEPLLRVTP
Sbjct: 361 WSKGGPHPGKFGWNHINDEFLNTIRFESTC--ANNSSSICFLFARKFLPNTLEPLLRVTP 413

BLAST of Lag0000250 vs. ExPASy TrEMBL
Match: A0A6J1D334 (uncharacterized protein LOC111016883 OS=Momordica charantia OX=3673 GN=LOC111016883 PE=4 SV=1)

HSP 1 Score: 633.3 bits (1632), Expect = 7.4e-178
Identity = 320/423 (75.65%), Postives = 357/423 (84.40%), Query Frame = 0

Query: 1   MGFEQKQPQWPLKSPNPNFQLKHVFHFLFFLVGFSLGIILCLYFKTLFSLTSQPSSSIPT 60
           MGF+QKQPQW  K  NP+  LKHVFHFLFFLVGFSLGIILCLYFKT FSLT QP      
Sbjct: 1   MGFDQKQPQWIPKCHNPHLHLKHVFHFLFFLVGFSLGIILCLYFKT-FSLTPQP------ 60

Query: 61  PNSPSSFPTSPPSSLLASTSPPPPPPDRPQPRPPSVLVLSIPRNGSSSKSSSVSLEEHKS 120
              PSS P +P       +SPPPP P  P P   S  +L +     S+ SSSVSLE+  S
Sbjct: 61  --PPSSSPPTP-------SSPPPPQPPSPPPPTSSTTILVL-----SNASSSVSLEQQSS 120

Query: 121 LLHNMSDEELFWRASMVPRIVELSDKTVPKVAFMFLTAGPLPLALLWEKFFKGHNGFYSI 180
           LLHNM+DEELFWRA+MVPRI EL++ + PKVAFMFLTAGPLPLA+LWEKFFKGH+GFYSI
Sbjct: 121 LLHNMTDEELFWRATMVPRIEELNN-SAPKVAFMFLTAGPLPLAVLWEKFFKGHHGFYSI 180

Query: 181 YVHSHPSYAHEMPQNSVFYGTRIPSQPVYWGTATMIDAERRLLANALLDFSNQRFVLLSD 240
           YVH HPSYA E+PQ+SVFYG RIPSQPVYWGT++MIDAERRLLANALLD SNQRFVLLSD
Sbjct: 181 YVHPHPSYADEIPQDSVFYGRRIPSQPVYWGTSSMIDAERRLLANALLDLSNQRFVLLSD 240

Query: 241 SCIPLFNFNTIYNHLMSSNRSFLASFDDPRKSCRGRYNPQMSPEINITNWRKGSQWFEVH 300
           SCIPLFNFNTIYNHL++SN SFLASFDDPRKS RGRYNP+MSP +N+T+WRKGSQWFE+H
Sbjct: 241 SCIPLFNFNTIYNHLITSNLSFLASFDDPRKSGRGRYNPKMSPAVNVTDWRKGSQWFEIH 300

Query: 301 REVALSIVSDTKYYPIFKNYCLPPCYMDEHYIPTLVTMLQPELNSNRSITWVDWSKGGPH 360
           R++AL IVSDT+YYPIFK++CLPPCYMDEHYIPTLV ML+P+LN NRSITWVDWSKGGPH
Sbjct: 301 RKLALRIVSDTRYYPIFKSHCLPPCYMDEHYIPTLVNMLEPQLNCNRSITWVDWSKGGPH 360

Query: 361 PSKFGWNDITDEFLNRIRFESTCNEG---QNSTSICFLFARKFLPNTLEPLLRVTPLLLC 420
           P KFGWNDITDEFLN IRFESTC++     +STSICFLFARKFLPNTLEPLLR+ PLLL 
Sbjct: 361 PRKFGWNDITDEFLNXIRFESTCDQDHHQNSSTSICFLFARKFLPNTLEPLLRIVPLLLG 401

BLAST of Lag0000250 vs. TAIR 10
Match: AT5G16170.1 (Core-2/I-branching beta-1,6-N-acetylglucosaminyltransferase family protein )

HSP 1 Score: 402.9 bits (1034), Expect = 3.1e-112
Identity = 225/416 (54.09%), Postives = 270/416 (64.90%), Query Frame = 0

Query: 23  HVFHFLFFLVGFSLGIILCLYFKTLFSLTSQPSSSIPTPNSPSSFPTSPPSSLLASTSPP 82
           +V   LFF+ GFS+G+ LCL  K +        S+  T   P        S+LL + +  
Sbjct: 32  YVLSSLFFVFGFSVGLFLCLQLKAVH------MSTTTTQRQPLW------STLLFNHTVH 91

Query: 83  PPPPDRPQPRPPSVLVLSIPRNGSSSKSSSVSLEEHKSLLHNMSDEELFWRASMVP---- 142
               D  Q      L L +                   L HNMSD+ELF +AS +     
Sbjct: 92  TTEMDIKQE-----LQLQV-------------------LQHNMSDQELFTKASSLSSPTS 151

Query: 143 ---------RIVELSDKTVPKVAFMFLTAGPLPLALLWEKFFKGHNGFYSIYVHSHPSYA 202
                    R      K   KVAFMF+T G LPLA LWEKFF+GH GFYSIYVH++PS+ 
Sbjct: 152 SLSSSSWLGRRHNNDGKMAVKVAFMFMTGGRLPLAGLWEKFFEGHEGFYSIYVHTNPSFQ 211

Query: 203 HEMPQNSVFYGTRIPSQPVYWGTATMIDAERRLLANALLDFSNQRFVLLSDSCIPLFNFN 262
              P+ SVFY  RIPSQPVYWGT++M+DAE+RLLANALLD SNQRFVLLSDSCIPL+NF 
Sbjct: 212 DSFPETSVFYSRRIPSQPVYWGTSSMVDAEKRLLANALLDESNQRFVLLSDSCIPLYNFT 271

Query: 263 TIYNHLMSSNRSFLASFDDPRKSCRGRYNPQMSPEINITNWRKGSQWFEVHREVALSIVS 322
           TIY++L  +N SF+ SFDDPRKS RGRYN  M P INIT+WRKGSQWFE  RE+AL I+ 
Sbjct: 272 TIYDYLTGTNLSFIGSFDDPRKSGRGRYNHTMYPHINITHWRKGSQWFETTRELALHIIE 331

Query: 323 DTKYYPIFKNYCLPPCYMDEHYIPTLVTMLQPELNSNRSITWVDWSKGGPHPSKFGWNDI 382
           DT YY IF  +C PPCYMDEHYIPTLV ML  E+++NR++TWVDWSK GPHP +F W DI
Sbjct: 332 DTVYYKIFDQHCKPPCYMDEHYIPTLVHMLHGEMSANRTLTWVDWSKAGPHPGRFIWPDI 391

Query: 383 TDEFLNRIRFESTC----NEGQN-STSICFLFARKFLPNTLEPLLRVTPLLLCIDP 421
           TDEFLNRIRF+  C      G+N +TS CFLFARKF   TLEPLLR++P++L   P
Sbjct: 392 TDEFLNRIRFKEECVYFGRGGENVTTSKCFLFARKFTAETLEPLLRISPIVLGFGP 411

BLAST of Lag0000250 vs. TAIR 10
Match: AT5G11730.1 (Core-2/I-branching beta-1,6-N-acetylglucosaminyltransferase family protein )

HSP 1 Score: 384.8 bits (987), Expect = 8.9e-107
Identity = 179/316 (56.65%), Postives = 228/316 (72.15%), Query Frame = 0

Query: 101 IPRNGSSSKSSSVSLEEHKSLLHNMSDEELFWRASMVPRIVELSDKTVPKVAFMFLTAGP 160
           +P       S S  ++    L+HNMSDEEL WRAS  PR  E   K VPKVAFMFLT GP
Sbjct: 69  VPCREGEPNSLSKWIQPPAVLMHNMSDEELLWRASFWPRRKEYPFKRVPKVAFMFLTKGP 128

Query: 161 LPLALLWEKFFKGHNGFYSIYVHSHPSYAHEMPQNSVFYGTRIPSQPVYWGTATMIDAER 220
           LPLA LWE+F KGH G YS+Y+H HPS+  + P +SVF+  +IPSQ   WG  +M DAE+
Sbjct: 129 LPLASLWERFLKGHKGLYSVYLHPHPSFTAKFPASSVFHRRQIPSQVAEWGRMSMCDAEK 188

Query: 221 RLLANALLDFSNQRFVLLSDSCIPLFNFNTIYNHLMSSNRSFLASFDDPRKSCRGRYNPQ 280
           RLLANALLD SN+ FVL+S+SCIPL+NF TIY++L  S  SF+ +FDDP    RGRYN  
Sbjct: 189 RLLANALLDVSNEWFVLVSESCIPLYNFTTIYSYLSRSKHSFMGAFDDPGPFGRGRYNGN 248

Query: 281 MSPEINITNWRKGSQWFEVHREVALSIVSDTKYYPIFKNYCLPPCYMDEHYIPTLVTMLQ 340
           M PE+ +T WRKGSQWFEV+R++A +IV DT YYP FK +C P CY+DEHY PT++T+ +
Sbjct: 249 MEPEVPLTKWRKGSQWFEVNRDLAATIVKDTLYYPKFKEFCRPACYVDEHYFPTMLTIEK 308

Query: 341 PELNSNRSITWVDWSKGGPHPSKFGWNDITDEFLNRIRFESTCNEGQNSTSICFLFARKF 400
           P + +NRS+TWVDWS+GGPHP+ FG +DIT+ F  +I     C+    +TS+C+LFARKF
Sbjct: 309 PTVLANRSLTWVDWSRGGPHPATFGRSDITENFFGKIFDGRNCSYNGRNTSMCYLFARKF 368

Query: 401 LPNTLEPLLRVTPLLL 417
            P+ LEPLL + P +L
Sbjct: 369 APSALEPLLHIAPKIL 384

BLAST of Lag0000250 vs. TAIR 10
Match: AT5G25970.1 (Core-2/I-branching beta-1,6-N-acetylglucosaminyltransferase family protein )

HSP 1 Score: 372.1 bits (954), Expect = 5.9e-103
Identity = 175/296 (59.12%), Postives = 213/296 (71.96%), Query Frame = 0

Query: 121 LLHNMSDEELFWRASMVPRIVELSDKTVPKVAFMFLTAGPLPLALLWEKFFKGHNGFYSI 180
           L+HNMSDEEL W AS +PR  E     VPK+AFMFLT GPLPLA LWE+  KGH   YS+
Sbjct: 90  LMHNMSDEELLWGASFMPRRKEYPFNRVPKIAFMFLTMGPLPLAPLWERLLKGHEKLYSV 149

Query: 181 YVHSHPSYAHEMPQNSVFYGTRIPSQPVYWGTATMIDAERRLLANALLDFSNQRFVLLSD 240
           Y+HS  S + + P +SVFY   IPSQ   WG  TM DAERRLLANALLD SN+ FVLLS+
Sbjct: 150 YIHSPVSSSAKFPASSVFYRRHIPSQVAEWGRMTMCDAERRLLANALLDISNEWFVLLSE 209

Query: 241 SCIPLFNFNTIYNHLMSSNRSFLASFDDPRKSCRGRYNPQMSPEINITNWRKGSQWFEVH 300
           SCIPLFNF TIY ++  S  SF+ SFDDP    RGRY+  M+PE+ I  WRKGSQWFE++
Sbjct: 210 SCIPLFNFTTIYTYMTKSEHSFMGSFDDPGAYGRGRYHGNMAPEVFIDQWRKGSQWFEIN 269

Query: 301 REVALSIVSDTKYYPIFKNYCLPPCYMDEHYIPTLVTMLQPELNSNRSITWVDWSKGGPH 360
           RE+A+SIV DT YYP FK +C P CY+DEHY PT++T+ +P   +NRS+TWVDWS+GG H
Sbjct: 270 RELAVSIVKDTLYYPKFKEFCQPACYVDEHYFPTMLTIEKPAALANRSVTWVDWSRGGAH 329

Query: 361 PSKFGWNDITDEFLNRIRFESTCNEGQNSTSICFLFARKFLPNTLEPLLRVTPLLL 417
           P+ FG  DI +EF  RI     C      TS+C+LFARKF P+ LEPL+++ P LL
Sbjct: 330 PATFGAQDINEEFFARILKGDNCTYNGGYTSMCYLFARKFSPSALEPLVQIAPKLL 385

BLAST of Lag0000250 vs. TAIR 10
Match: AT1G10280.1 (Core-2/I-branching beta-1,6-N-acetylglucosaminyltransferase family protein )

HSP 1 Score: 358.2 bits (918), Expect = 8.9e-99
Identity = 165/299 (55.18%), Postives = 224/299 (74.92%), Query Frame = 0

Query: 119 KSLLHNMSDEELFWRASMVPRIVELSDKTVPKVAFMFLTAGPLPLALLWEKFFKGHNGFY 178
           ++L H M+D+ELFWRASMVP   E     VPKVAFMFLT GPLP+  LWEKFFKG+  + 
Sbjct: 112 ENLSHGMTDDELFWRASMVPVKEEYPYDRVPKVAFMFLTRGPLPMLPLWEKFFKGNEKYL 171

Query: 179 SIYVHSHPSYAHEMPQNSVFYGTRIPSQPVYWGTATMIDAERRLLANALLDFSNQRFVLL 238
           S+YVH+ P Y   + ++S FY  +IPSQ V WG+  + DAE+RLLANALLDFSN+RFVLL
Sbjct: 172 SVYVHTPPGYDMNVSRDSPFYDRQIPSQRVEWGSPLLTDAEKRLLANALLDFSNERFVLL 231

Query: 239 SDSCIPLFNFNTIYNHLMSSNRSFLASFDDPRKSCRGRYNPQMSPEINITNWRKGSQWFE 298
           S+SC+P++NF+T+Y +L++S  SF+ S+D+P +  RGRY+ +M P+I + +WRKGSQWFE
Sbjct: 232 SESCVPVYNFSTVYTYLINSAYSFVDSYDEPTRYGRGRYSRKMLPDIKLHHWRKGSQWFE 291

Query: 299 VHREVALSIVSDTKYYPIFKNYCLPPCYMDEHYIPTLVTMLQPELNSNRSITWVDWSKGG 358
           V+R++A+ I+SD+KYY +FK +C P CY DEHYIPT + M    +N+NRS+TWVDWS GG
Sbjct: 292 VNRKIAIYIISDSKYYSLFKQFCRPACYPDEHYIPTFLNMFHGSMNANRSVTWVDWSIGG 351

Query: 359 PHPSKFGWNDITDEFLNRIR-FESTCNEGQNSTSICFLFARKFLPNTLEPLLRVTPLLL 417
           PHP+ +   +IT+ FL  IR  E+ C   +  TS+CFLFARKF P+ L PL+ ++  +L
Sbjct: 352 PHPATYAAANITEGFLQSIRKNETDCLYNEEPTSLCFLFARKFSPSALAPLMNLSSTVL 410

BLAST of Lag0000250 vs. TAIR 10
Match: AT3G21310.1 (Core-2/I-branching beta-1,6-N-acetylglucosaminyltransferase family protein )

HSP 1 Score: 354.8 bits (909), Expect = 9.8e-98
Identity = 160/294 (54.42%), Postives = 213/294 (72.45%), Query Frame = 0

Query: 123 HNMSDEELFWRASMVPRIVELSDKTVPKVAFMFLTAGPLPLALLWEKFFKGHNGFYSIYV 182
           H+M+D EL WRASM PRI++   K VPK+AFMFLT GPLP A LWE+FFKGH GFYSIYV
Sbjct: 88  HSMNDSELLWRASMEPRILDYPFKRVPKMAFMFLTKGPLPFAPLWERFFKGHEGFYSIYV 147

Query: 183 HSHPSYAHEMPQNSVFYGTRIPSQPVYWGTATMIDAERRLLANALLDFSNQRFVLLSDSC 242
           H+ P+Y  + P +SVFY  +IPSQ V WG  +M DAERRLLANALLD SN+ FVLLS++C
Sbjct: 148 HTLPNYRSDFPSSSVFYRRQIPSQHVAWGEMSMCDAERRLLANALLDISNEWFVLLSEAC 207

Query: 243 IPLFNFNTIYNHLMSSNRSFLASFDDPRKSCRGRYNPQMSPEINITNWRKGSQWFEVHRE 302
           IPL  FN +Y ++  S  SF+ S D+     RGRY+  M PE+++  WRKGSQWFE++R 
Sbjct: 208 IPLRGFNFVYRYVSRSRYSFMGSVDEDGPYGRGRYSYAMGPEVSLNEWRKGSQWFEINRA 267

Query: 303 VALSIVSDTKYYPIFKNYCLPPCYMDEHYIPTLVTMLQPELNSNRSITWVDWSKGGPHPS 362
           +A+ IV D  YY  FK +C PPCY+DEHY PT++++  P+  +NR++TW DWS+GG HP+
Sbjct: 268 LAVDIVEDMVYYNKFKEFCRPPCYVDEHYFPTMLSIGYPDFLANRTLTWTDWSRGGAHPA 327

Query: 363 KFGWNDITDEFLNRIRFESTCNEGQNSTSICFLFARKFLPNTLEPLLRVTPLLL 417
            FG  DIT++F+ ++     C      + +C+LFARKF P+ L+PLL++ P +L
Sbjct: 328 TFGKADITEKFIKKLSRGKACFYNDQPSQVCYLFARKFAPSALKPLLKLAPKVL 381

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038887114.13.4e-19382.57glycosyltransferase BC10-like [Benincasa hispida][more]
KAA0048474.19.0e-18679.86Core-2/I-branching beta-1,6-N-acetylglucosaminyltransferase family protein [Cucu... [more]
XP_008461555.12.0e-18579.86PREDICTED: uncharacterized protein LOC103500127 [Cucumis melo][more]
XP_011651351.11.2e-18277.60glycosyltransferase BC10 [Cucumis sativus] >KGN57704.1 hypothetical protein Csa_... [more]
KAG7017896.14.8e-17977.75hypothetical protein SDJN02_19762, partial [Cucurbita argyrosperma subsp. argyro... [more]
Match NameE-valueIdentityDescription
Q65XS55.2e-4338.72Glycosyltransferase BC10 OS=Oryza sativa subsp. japonica OX=39947 GN=BC10 PE=1 S... [more]
Match NameE-valueIdentityDescription
A0A5D3DYE74.4e-18679.86Core-2/I-branching beta-1,6-N-acetylglucosaminyltransferase family protein OS=Cu... [more]
A0A1S3CF009.7e-18679.86uncharacterized protein LOC103500127 OS=Cucumis melo OX=3656 GN=LOC103500127 PE=... [more]
A0A0A0L9P15.9e-18377.60Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G257090 PE=4 SV=1[more]
A0A6J1F2L42.6e-17877.52uncharacterized protein LOC111441621 OS=Cucurbita moschata OX=3662 GN=LOC1114416... [more]
A0A6J1D3347.4e-17875.65uncharacterized protein LOC111016883 OS=Momordica charantia OX=3673 GN=LOC111016... [more]
Match NameE-valueIdentityDescription
AT5G16170.13.1e-11254.09Core-2/I-branching beta-1,6-N-acetylglucosaminyltransferase family protein [more]
AT5G11730.18.9e-10756.65Core-2/I-branching beta-1,6-N-acetylglucosaminyltransferase family protein [more]
AT5G25970.15.9e-10359.12Core-2/I-branching beta-1,6-N-acetylglucosaminyltransferase family protein [more]
AT1G10280.18.9e-9955.18Core-2/I-branching beta-1,6-N-acetylglucosaminyltransferase family protein [more]
AT3G21310.19.8e-9854.42Core-2/I-branching beta-1,6-N-acetylglucosaminyltransferase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003406Glycosyl transferase, family 14PFAMPF02485Branchcoord: 151..378
e-value: 1.4E-84
score: 283.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 58..74
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 75..96
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 58..113
NoneNo IPR availablePANTHERPTHR31042:SF20CORE-2/I-BRANCHING BETA-1,6-N-ACETYLGLUCOSAMINYLTRANSFERASE FAMILY PROTEINcoord: 12..416
IPR044174Glycosyltransferase BC10-likePANTHERPTHR31042CORE-2/I-BRANCHING BETA-1,6-N-ACETYLGLUCOSAMINYLTRANSFERASE FAMILY PROTEIN-RELATEDcoord: 12..416

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0000250.1Lag0000250.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0016757 glycosyltransferase activity