Lag0000212 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0000212
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Reverse transcriptase domain-containing protein
Location	chr4: 1578374 .. 1581088 (+)
RNA-Seq Expression	Lag0000212
Synteny	Lag0000212

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCTCGCTATCAATGCCACGGTGGCAGGACTGATGACGGCCTCGAGGGGTGGGACCAAACCCTGTTGGAACAACACTTTAATGCCGCAGAGGTAAGCCTCATTTTATATATCCCCATTAGACAGCACCCCCCTAAGGACTCAGTTATCTGGCATTATGAAAAGTTTGGCCTCTTCTCGGTTAAAAGTGGATACAAGTTGGCTCAGTCAGTCACCCTGGCACGAGGGGCTTCATCTTCATCCCCTAACTCTCTCCAGGATTGGTGGAAGGGATGTTGGAAGATGACGCTCCCAAGTAAGATAAAGGTGTTCCTTTGGAGATTGTGTCTGAACAGGTTGCCGACAATTGACAATCTGATAAGAAGGGGGTGGACCTGCTAGATGTATGCGTTTTCTGCAGGAAGCGGGGGAATCTAGCATGCACTTTTTTGGGAATGCAAATGGGTCAGATCGATCCTACGAGAAGCGGGTTTTGGGGAGATCTTGGAGAGAGTCCGAGCAGGATGTTGTCTACTACTCTGTCAGGACATCAAAGAGGCGATAGGGGGAGAATTTGAGGAACTGGTGGTGTTATGGTGGTCGATGTGGTCGACCTGGAATAAAGTTCGTTTTCAAGGGGCTGATAGGCCGAGTGGGCTAGTGGAATGGGCTAAGGGGTATGTGGTGGCTTTTCGTGAGGTTGGGAGGAGTAGTAGGGAAGTGGGTCGGGAGACGGTTGGTCGGGAGAGGGTAGTGTGGCGGGCCCCGAGGAATGGCTGGTATAAGGTGAATTTTGATGCCTCTTTCCTCTCTCATTTGTCCAGGGTCGACCTAGGGATAATTGTGCGGGATCCTTTGGGCCAAGTAATGTTGTCGGCGACTTTTACTAAGGATAATGTGAGAGAGGTTGACATGGCTGAAGGATATGCAGCCGTGAAAAGTTTGGAGTTGGTGACAGATATGGGTTTGGGTCCAGCAATTCTTGAGACGGACTCGAGTAGAGTATTCCAGATCCTTAACCGAGAACGTGAGGATATCTCTGAGCTAGGTATGATTATTGCAGATGCACTAATGGCTTTTCCTTCTAATTTGCAGGTACTTTTCATTTCGTTCACCGTGAAGGGAATCAGGCGGCGCACCGATTGGCGAGGTTAGCAATAGCCCAGTGGAGTGATGAGGTTTGGATGGAGACTTGTCCTAGGTGTCTGGAGGACGTAATTTTGAACGATTCAGCGTACATGGAGGCATGAGGTGGGGACTAAGCTATGCAATTTCGGCCTATATGCCAACTGACGTGACATGCAATCAGGGGAGGTTTCTGCTCTCCTTTCTTTTTGTTGTGTATGCCTGATGTGGACTGCATCAGGGAGATTGCATGTTCATGGGGGTTTTGGGTTGTTCTTGCATGCTAGTGGATATTAGACTGACGATGTTGCATATGAGCACCAGGACATTATTAATTCATGTTTATTTACGTGTTTCTATGAATCTTGGTGGGGGTCATGCATCTGAGCAGTGGACCTTTTGCTTCTGGGATCAATGGAAGCGTGGTGAATTCTAATAGCTGAGGTCTGGGTTTGGGGTTGAGTCATGATACTGCTGTCGAGTACTCGTATACTTTTCTTTGGTGTATAGGTGAGGTTTAGCTTGCTAGCATAATTTTGGGGCCAGTTTATTGCTTTCTGAATGTCTTGCACATTTTGAGCACTTGGCTTGTCCTTTATGTAGATTGAAGGCCTAGACCTGCTTTATGGGGTTCATAAGTCTGTTGTCACTCCGATAGGGGTAAGGTTTCGCTAGGGAGTATTTGATCTTTGTAGTTTGGGTGTTGTCTTGGCCTTTGGGGAATTGAGAGGTTTGAAAAAGAAAAAAAAAAAAAGAAAAAAAAGGAAAAGATATGGAAAAGTGAAAATGCGAAAAAGAAGGAAAGAAATGGAAAAGCGAAAATGCGAAAAAGAAAGAAAAGAAATGAAAAGCATGAGTGGTTTGGGGCTTGGGGGTTTCCTTTGGGGAAAGTACATGCACACTGTTGTTTTCCTAAAAGCTTATTATGTGTCTTGATTATTCATATGCATGGGGTAAAAAGAGCTACCTTCAGTGTGTTCAGTTAGGCAAACCAAGGGTTGCAGTCTGGGTTAGGGGTCCCTGCACTGTAGTGTGAAAGCTAGTGCCCTTGATTGTAGCATCAGTGTGCGTTAGGATAAGGGAAAGGAAAGTGAATCCTTTTCTGGTTCCTGGGTTTTGAGATAGCATGCTAAATCTGCGCCAGTCGGTCTCCATTGCGAAACCTTTGTTGCCAATGTAGGGGGAAGATAGGTGGAGGATCCCATAAATCTGTATCTGGTCCATTAGAGGCTTCTTTAGGTAGGCTAATCACACACACACGCGAGTCTCTTGGAAAGCTTTGATCTGTTTCCTTATTTTACTAGTTGTGATAACTTCTCTTAGGCTAAAGGTTGAGTAGATGTAGTCCAGGATAGTGAGTATGTTTTCAACCCCCTAAAGTCTACTATTGGTATTTCATTCAGTGGGTATGAATTACATGTGGATTGATGAGGTTAGGTTAGGGGTGATGGGGTTTGGTTTTTTTCCGTCTAAGCTACTTATCTCTTTGATGTCTTAAGATGTTTTGTTCGGGTGTTCTGTTACAGGTGGTGAGCCTAAGGCCACAAACCTACATGGAACAATTCATATTTATCTAGGACGCAAGAGGATGGAGGTTTGGATAGCTAGCTGA

mRNA sequence

ATGCTCGCTATCAATGCCACGGTGGCAGGACTGATGACGGCCTCGAGGGGTGGGACCAAACCCTGTTGGAACAACACTTTAATGCCGCAGAGTGGATACAAGTTGGCTCAGTCAGTCACCCTGGCACGAGGGGCTTCATCTTCATCCCCTAACTCTCTCCAGGATTGGTGGAAGGGATGTTGGAAGATGACGCTCCCAAGTAAGATAAAGGTGTTCCTTTGGAGATTGTGTCTGAACAGATCGATCCTACGAGAAGCGGGTTTTGGGGAGATCTTGGAGAGAGTCCGAGCAGGATGTTGTCTACTACTCTGTCAGGACATCAAAGAGGCGATAGGGGGAGAATTTGAGGAACTGGTGGTGTTATGGTGGTCGATGTGGTCGACCTGGAATAAAGTTCGTTTTCAAGGGGCTGATAGGCCGAGTGGGCTAGTGGAATGGGCTAAGGGGTATGTGGTGGCTTTTCGTGAGGTTGGGAGGAGTAGTAGGGAAGTGGGTCGGGAGACGGTTGGTCGGGAGAGGGTAGTGTGGCGGGCCCCGAGGAATGGCTGGTATAAGGTGAATTTTGATGCCTCTTTCCTCTCTCATTTGTCCAGGGTCGACCTAGGGATAATTGTGCGGGATCCTTTGGGCCAAGTAATGTTGTCGGCGACTTTTACTAAGGATAATGTGAGAGAGGTTGACATGGCTGAAGGATATGCAGCCGTGAAAAGTTTGGAGTTGGTGACAGATATGGGTTTGGGTCCAGCAATTCTTGAGACGGACTCGAGTAGAGTATTCCAGATCCTTAACCGAGAACGTGAGGATATCTCTGAGCTAGGTACTTTTCATTTCGTTCACCGTGAAGGGAATCAGGCGGCGCACCGATTGGCGAGGTTAGCAATAGCCCAGTGGAGTGATGAGGTTTGGATGGAGACTTGTCCTAGGTGTCTGGAGGACATTGAAGGCCTAGACCTGCTTTATGGGGTTCATAAGTCTGTTGTCACTCCGATAGGGGTAAGGGGGAAGATAGGTGGAGGATCCCATAAATCTGTATCTGGTCCATTAGAGGCTTCTTTAGGTGGTGAGCCTAAGGCCACAAACCTACATGGAACAATTCATATTTATCTAGGACGCAAGAGGATGGAGGTTTGGATAGCTAGCTGA

Coding sequence (CDS)

ATGCTCGCTATCAATGCCACGGTGGCAGGACTGATGACGGCCTCGAGGGGTGGGACCAAACCCTGTTGGAACAACACTTTAATGCCGCAGAGTGGATACAAGTTGGCTCAGTCAGTCACCCTGGCACGAGGGGCTTCATCTTCATCCCCTAACTCTCTCCAGGATTGGTGGAAGGGATGTTGGAAGATGACGCTCCCAAGTAAGATAAAGGTGTTCCTTTGGAGATTGTGTCTGAACAGATCGATCCTACGAGAAGCGGGTTTTGGGGAGATCTTGGAGAGAGTCCGAGCAGGATGTTGTCTACTACTCTGTCAGGACATCAAAGAGGCGATAGGGGGAGAATTTGAGGAACTGGTGGTGTTATGGTGGTCGATGTGGTCGACCTGGAATAAAGTTCGTTTTCAAGGGGCTGATAGGCCGAGTGGGCTAGTGGAATGGGCTAAGGGGTATGTGGTGGCTTTTCGTGAGGTTGGGAGGAGTAGTAGGGAAGTGGGTCGGGAGACGGTTGGTCGGGAGAGGGTAGTGTGGCGGGCCCCGAGGAATGGCTGGTATAAGGTGAATTTTGATGCCTCTTTCCTCTCTCATTTGTCCAGGGTCGACCTAGGGATAATTGTGCGGGATCCTTTGGGCCAAGTAATGTTGTCGGCGACTTTTACTAAGGATAATGTGAGAGAGGTTGACATGGCTGAAGGATATGCAGCCGTGAAAAGTTTGGAGTTGGTGACAGATATGGGTTTGGGTCCAGCAATTCTTGAGACGGACTCGAGTAGAGTATTCCAGATCCTTAACCGAGAACGTGAGGATATCTCTGAGCTAGGTACTTTTCATTTCGTTCACCGTGAAGGGAATCAGGCGGCGCACCGATTGGCGAGGTTAGCAATAGCCCAGTGGAGTGATGAGGTTTGGATGGAGACTTGTCCTAGGTGTCTGGAGGACATTGAAGGCCTAGACCTGCTTTATGGGGTTCATAAGTCTGTTGTCACTCCGATAGGGGTAAGGGGGAAGATAGGTGGAGGATCCCATAAATCTGTATCTGGTCCATTAGAGGCTTCTTTAGGTGGTGAGCCTAAGGCCACAAACCTACATGGAACAATTCATATTTATCTAGGACGCAAGAGGATGGAGGTTTGGATAGCTAGCTGA

Protein sequence

MLAINATVAGLMTASRGGTKPCWNNTLMPQSGYKLAQSVTLARGASSSSPNSLQDWWKGCWKMTLPSKIKVFLWRLCLNRSILREAGFGEILERVRAGCCLLLCQDIKEAIGGEFEELVVLWWSMWSTWNKVRFQGADRPSGLVEWAKGYVVAFREVGRSSREVGRETVGRERVVWRAPRNGWYKVNFDASFLSHLSRVDLGIIVRDPLGQVMLSATFTKDNVREVDMAEGYAAVKSLELVTDMGLGPAILETDSSRVFQILNREREDISELGTFHFVHREGNQAAHRLARLAIAQWSDEVWMETCPRCLEDIEGLDLLYGVHKSVVTPIGVRGKIGGGSHKSVSGPLEASLGGEPKATNLHGTIHIYLGRKRMEVWIAS

Homology

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BLAST of Lag0000212 vs. NCBI nr
Match: XP_022150918.1 (uncharacterized protein LOC111018954 [Momordica charantia])

HSP 1 Score: 172.6 bits (436), Expect = 6.7e-39
Identity = 115/325 (35.38%), Postives = 166/325 (51.08%), Query Frame = 0


Query: 30   QSGYKLA-QSVTLARGASSSSPNSLQDWWKGCWKMTLPSKIKVFLWRLCLNR----SILR 89
            +SGYK+A  +    +  SSSS   ++ WW G WKM +P+KIKVFLWRLCL+R      L 
Sbjct: 808  RSGYKVALLNNPCVQAPSSSSSEEVRCWWNGFWKMHIPNKIKVFLWRLCLDRLPTGCNLS 867

Query: 90   EAG-------------------------FGEIL----ERVRAGCCLLLCQDIKEAIGGEF 149
            + G                         F E L    +  +    L+L +  +     +F
Sbjct: 868  KRGVEITNCCYFCGRNGEDSIHLFWICKFAEALWINSKFGKLSPFLILRESHESLSKADF 927

Query: 150  EELVVLWWSMWSTWNKVRFQGADRP-----SGLVEWAKGYVVAFREVGRSSREVGRETVG 209
            EEL V+ W +W+  N   F  + +        LVEWA  Y + FRE  +S+   GR T  
Sbjct: 928  EELCVVIWGLWNQRNARAFNDSTKTVFKIGMELVEWANKYAMEFRE-AKSNPITGRVTNT 987

Query: 210  RERVVWRAPRNGWYKVNFDASFLSHLSRVDLGIIVRDPLGQVMLSATFTKDNVREVDMAE 269
             E ++W+ P  G YK+N DASFL+      LGII+ +  GQVM +AT   +N++ VDMAE
Sbjct: 988  AE-ILWQPPDEGIYKINTDASFLASDQHAGLGIIIHNDRGQVMAAATKYLENIQSVDMAE 1047

Query: 270  GYAAVKSLELVTDMGLGPAILETDSSRVFQILNREREDISELGTFHFVHREGNQAAHRLA 312
              AAV+ L+L +++G+ PA+ +   +    +  +     S   +F+FV REGN+AAH LA
Sbjct: 1048 AIAAVEGLQLASEIGMHPALEDLSETGEIVLKAKNFWTQSLHASFNFVKREGNKAAHMLA 1107

[Close]

BLAST of Lag0000212 vs. NCBI nr
Match: XP_022140628.1 (uncharacterized protein LOC111011237 [Momordica charantia])

HSP 1 Score: 142.5 bits (358), Expect = 7.4e-30
Identity = 85/189 (44.97%), Postives = 110/189 (58.20%), Query Frame = 0


Query: 143 LVEWAKGYVVAFREVGRSSREVGRETVGRERVVWRAPRNGWYKVNFDASFLSHLSRVDLG 202
           LVEWA  YV+ FRE   S+   GR T   E V+W  P    YK+N DASFL+      LG
Sbjct: 3   LVEWANKYVMEFRE-ANSNPFPGRVTNTAE-VLWLPPDKRIYKINTDASFLASDQHAGLG 62

Query: 203 IIVRDPLGQVMLSATFTKDNVREVDMAEGYAAVKSLELVTDMGLGPAILETDSSRVFQIL 262
           II+R+  GQVM SAT   +N++ VDMAE   AV+ L+L + +G+ P ILETDSSR+F + 
Sbjct: 63  IIIRNDRGQVMASATKYLENIQSVDMAEAIVAVEGLQLASKIGVNPVILETDSSRIFNLF 122

Query: 263 NREREDISELG----------------TFHFVHREGNQAAHRLARLAIAQWSDEVWMETC 312
           ++  ED+SE G                +F+FV REGN+AAH LAR A+      +WME  
Sbjct: 123 SQPSEDLSETGEIVLKAKNFWTQSLHASFNFVKREGNKAAHMLARRALLLREFSIWMEDW 182

[Close]

BLAST of Lag0000212 vs. NCBI nr
Match: KAF4395712.1 (hypothetical protein G4B88_013486 [Cannabis sativa])

HSP 1 Score: 125.2 bits (313), Expect = 1.2e-24
Identity = 81/315 (25.71%), Postives = 139/315 (44.13%), Query Frame = 0


Query: 30  QSGYKLAQSVTLARGASSSSPNSLQDWWKGCWKMTLPSKIKVFLWRL------------- 89
           ++GY +A+        SSS+  +L  WWK  W++ +P KI+ F++RL             
Sbjct: 280 RTGYHVAKQAINRVQPSSSNTETLTRWWKSLWRLPIPPKIRHFVYRLAQHSLPTTNNLYN 339

Query: 90  --CLNRSILREAGFGEILERVRAGCCLLLCQDIKEAIGGEFEELVVLWWSMWSTWNKVRF 149
             C++  I          E V+    L  CQ++K+A   EF   + + W  W+  N   F
Sbjct: 340 RHCISSPICPRCSL--CFESVQH--ALFECQEMKKAWSDEFNLFLCMLWKCWNARNASVF 399

Query: 150 QG-ADRPSGLVEWAKGYVVAFREVGRSSREVGRETVGRERVVWRAPRNGWYKVNFDASFL 209
           +    RP  + + A+ Y+  ++          + T  R+ +VW  P  G+ K+N DA+  
Sbjct: 400 RNQVSRPETIEQEAQDYLAFYQAAQDKRGNHSQSTSDRDLLVWEPPPVGFLKLNTDAAIS 459

Query: 210 SHLSRVDLGIIVRDPLGQVMLSATFTKDNVREVDMAEGYAAVKSLELVTDMGLGPAILET 269
           SH +R   G +VRD  G+++ +  F +        AEG+A +++L+   D G+    +E 
Sbjct: 460 SHQNRTGGGALVRDHTGKIIAATAFNRIGQLHPQAAEGWALLEALKWCQDKGININHVEV 519

Query: 270 DSSRVFQILNREREDISELG----------------TFHFVHREGNQAAHRLARLAIAQW 313
           D   +   L    E+++  G                T H   R+GN AAH LA+++I   
Sbjct: 520 DCKNLLTELQTSNENLTSFGNIINAIRRLLSSFPTVTLHHTRRQGNTAAHNLAQVSIG-- 579

[Close]

BLAST of Lag0000212 vs. NCBI nr
Match: KAF4386115.1 (hypothetical protein F8388_016367 [Cannabis sativa])

HSP 1 Score: 125.2 bits (313), Expect = 1.2e-24
Identity = 81/315 (25.71%), Postives = 139/315 (44.13%), Query Frame = 0


Query: 30  QSGYKLAQSVTLARGASSSSPNSLQDWWKGCWKMTLPSKIKVFLWRL------------- 89
           ++GY +A+        SSS+  +L  WWK  W++ +P KI+ F++RL             
Sbjct: 305 RTGYHVAKQAINRVQPSSSNTETLTRWWKSLWRLPIPPKIRHFVYRLAQHSLPTTNNLYN 364

Query: 90  --CLNRSILREAGFGEILERVRAGCCLLLCQDIKEAIGGEFEELVVLWWSMWSTWNKVRF 149
             C++  I          E V+    L  CQ++K+A   EF   + + W  W+  N   F
Sbjct: 365 RHCISSPICPRCSL--CFESVQH--ALFECQEMKKAWSDEFNLFLCMLWKCWNARNASVF 424

Query: 150 QG-ADRPSGLVEWAKGYVVAFREVGRSSREVGRETVGRERVVWRAPRNGWYKVNFDASFL 209
           +    RP  + + A+ Y+  ++          + T  R+ +VW  P  G+ K+N DA+  
Sbjct: 425 RNQVSRPETIEQEAQDYLAFYQAAQDKRGNHSQSTSDRDLLVWEPPPVGFLKLNTDAAIS 484

Query: 210 SHLSRVDLGIIVRDPLGQVMLSATFTKDNVREVDMAEGYAAVKSLELVTDMGLGPAILET 269
           SH +R   G +VRD  G+++ +  F +        AEG+A +++L+   D G+    +E 
Sbjct: 485 SHQNRTGGGALVRDHTGKIIAATAFNRIGQLHPQAAEGWALLEALKWCQDKGININHVEV 544

Query: 270 DSSRVFQILNREREDISELG----------------TFHFVHREGNQAAHRLARLAIAQW 313
           D   +   L    E+++  G                T H   R+GN AAH LA+++I   
Sbjct: 545 DCKNLLTELQTSNENLTSFGNIINAIRRLLSSFPTVTLHHTRRQGNTAAHNLAQVSIG-- 604

[Close]

BLAST of Lag0000212 vs. NCBI nr
Match: KAF4364303.1 (hypothetical protein G4B88_028423 [Cannabis sativa])

HSP 1 Score: 124.4 bits (311), Expect = 2.1e-24
Identity = 80/305 (26.23%), Postives = 137/305 (44.92%), Query Frame = 0


Query: 30  QSGYKLAQSVTLARGASSSSPNSLQDWWKGCWKMTLPSKIKVFLWRL------------- 89
           ++GY +A+        SSS+  +L  WWK  W++ +P KI+ F++RL             
Sbjct: 403 RTGYHVAKQAINRVQPSSSNTETLTRWWKSLWRLPIPPKIRHFVYRLAQHSLPTTNNLYN 462

Query: 90  --CLNRSILREAGFGEILERVRAGCCLLLCQDIKEAIGGEFEELVVLWWSMWSTWNKVRF 149
             C++  I  +       E V+    L  CQ++K+A   EF   + + W  W+  N   F
Sbjct: 463 RHCISSPICPKCSL--CFESVQH--ALFECQEMKKAWSDEFNLFLCMLWKCWNARNASVF 522

Query: 150 QG-ADRPSGLVEWAKGYVVAFREVGRSSREVGRETVGRERVVWRAPRNGWYKVNFDASFL 209
           +    RP  + + A+ Y+  ++          + T  R+ +VW  P  G+ K+N DA+  
Sbjct: 523 RNQVSRPETIEQEAQDYLAFYQAAQDKRWNHSQSTSDRDLLVWEPPPVGFLKLNTDAAIS 582

Query: 210 SHLSRVDLGIIVRDPLGQVMLSATFTKDNVREVDMAEGYAAVKSLELVTDMGLGPAILET 269
           SH +R   G +VRD  G+++ +  F +        AEG+A +++L+   D G+    +E 
Sbjct: 583 SHQNRTGGGALVRDHTGKIIAATAFNRIGQLHPQAAEGWALLEALKWCQDKGININHVEV 642

Query: 270 DSSRVFQILNREREDISELG----------------TFHFVHREGNQAAHRLARLAIAQW 303
           D   +   L    E+++  G                T H   R+GN AAH LA+++I   
Sbjct: 643 DCKNLLTELQTSSENLTSFGNIINAIRRLLSSFPTVTLHHTRRQGNTAAHNLAQMSIG-- 701

[Close]

BLAST of Lag0000212 vs. ExPASy TrEMBL
Match: A0A6J1DAR4 (uncharacterized protein LOC111018954 OS=Momordica charantia OX=3673 GN=LOC111018954 PE=4 SV=1)

HSP 1 Score: 172.6 bits (436), Expect = 3.3e-39
Identity = 115/325 (35.38%), Postives = 166/325 (51.08%), Query Frame = 0


Query: 30   QSGYKLA-QSVTLARGASSSSPNSLQDWWKGCWKMTLPSKIKVFLWRLCLNR----SILR 89
            +SGYK+A  +    +  SSSS   ++ WW G WKM +P+KIKVFLWRLCL+R      L 
Sbjct: 808  RSGYKVALLNNPCVQAPSSSSSEEVRCWWNGFWKMHIPNKIKVFLWRLCLDRLPTGCNLS 867

Query: 90   EAG-------------------------FGEIL----ERVRAGCCLLLCQDIKEAIGGEF 149
            + G                         F E L    +  +    L+L +  +     +F
Sbjct: 868  KRGVEITNCCYFCGRNGEDSIHLFWICKFAEALWINSKFGKLSPFLILRESHESLSKADF 927

Query: 150  EELVVLWWSMWSTWNKVRFQGADRP-----SGLVEWAKGYVVAFREVGRSSREVGRETVG 209
            EEL V+ W +W+  N   F  + +        LVEWA  Y + FRE  +S+   GR T  
Sbjct: 928  EELCVVIWGLWNQRNARAFNDSTKTVFKIGMELVEWANKYAMEFRE-AKSNPITGRVTNT 987

Query: 210  RERVVWRAPRNGWYKVNFDASFLSHLSRVDLGIIVRDPLGQVMLSATFTKDNVREVDMAE 269
             E ++W+ P  G YK+N DASFL+      LGII+ +  GQVM +AT   +N++ VDMAE
Sbjct: 988  AE-ILWQPPDEGIYKINTDASFLASDQHAGLGIIIHNDRGQVMAAATKYLENIQSVDMAE 1047

Query: 270  GYAAVKSLELVTDMGLGPAILETDSSRVFQILNREREDISELGTFHFVHREGNQAAHRLA 312
              AAV+ L+L +++G+ PA+ +   +    +  +     S   +F+FV REGN+AAH LA
Sbjct: 1048 AIAAVEGLQLASEIGMHPALEDLSETGEIVLKAKNFWTQSLHASFNFVKREGNKAAHMLA 1107

[Close]

BLAST of Lag0000212 vs. ExPASy TrEMBL
Match: A0A6J1CIF1 (uncharacterized protein LOC111011237 OS=Momordica charantia OX=3673 GN=LOC111011237 PE=4 SV=1)

HSP 1 Score: 142.5 bits (358), Expect = 3.6e-30
Identity = 85/189 (44.97%), Postives = 110/189 (58.20%), Query Frame = 0


Query: 143 LVEWAKGYVVAFREVGRSSREVGRETVGRERVVWRAPRNGWYKVNFDASFLSHLSRVDLG 202
           LVEWA  YV+ FRE   S+   GR T   E V+W  P    YK+N DASFL+      LG
Sbjct: 3   LVEWANKYVMEFRE-ANSNPFPGRVTNTAE-VLWLPPDKRIYKINTDASFLASDQHAGLG 62

Query: 203 IIVRDPLGQVMLSATFTKDNVREVDMAEGYAAVKSLELVTDMGLGPAILETDSSRVFQIL 262
           II+R+  GQVM SAT   +N++ VDMAE   AV+ L+L + +G+ P ILETDSSR+F + 
Sbjct: 63  IIIRNDRGQVMASATKYLENIQSVDMAEAIVAVEGLQLASKIGVNPVILETDSSRIFNLF 122

Query: 263 NREREDISELG----------------TFHFVHREGNQAAHRLARLAIAQWSDEVWMETC 312
           ++  ED+SE G                +F+FV REGN+AAH LAR A+      +WME  
Sbjct: 123 SQPSEDLSETGEIVLKAKNFWTQSLHASFNFVKREGNKAAHMLARRALLLREFSIWMEDW 182

[Close]

BLAST of Lag0000212 vs. ExPASy TrEMBL
Match: A0A7J6HKE1 (Uncharacterized protein OS=Cannabis sativa OX=3483 GN=G4B88_013486 PE=4 SV=1)

HSP 1 Score: 125.2 bits (313), Expect = 6.0e-25
Identity = 81/315 (25.71%), Postives = 139/315 (44.13%), Query Frame = 0


Query: 30  QSGYKLAQSVTLARGASSSSPNSLQDWWKGCWKMTLPSKIKVFLWRL------------- 89
           ++GY +A+        SSS+  +L  WWK  W++ +P KI+ F++RL             
Sbjct: 280 RTGYHVAKQAINRVQPSSSNTETLTRWWKSLWRLPIPPKIRHFVYRLAQHSLPTTNNLYN 339

Query: 90  --CLNRSILREAGFGEILERVRAGCCLLLCQDIKEAIGGEFEELVVLWWSMWSTWNKVRF 149
             C++  I          E V+    L  CQ++K+A   EF   + + W  W+  N   F
Sbjct: 340 RHCISSPICPRCSL--CFESVQH--ALFECQEMKKAWSDEFNLFLCMLWKCWNARNASVF 399

Query: 150 QG-ADRPSGLVEWAKGYVVAFREVGRSSREVGRETVGRERVVWRAPRNGWYKVNFDASFL 209
           +    RP  + + A+ Y+  ++          + T  R+ +VW  P  G+ K+N DA+  
Sbjct: 400 RNQVSRPETIEQEAQDYLAFYQAAQDKRGNHSQSTSDRDLLVWEPPPVGFLKLNTDAAIS 459

Query: 210 SHLSRVDLGIIVRDPLGQVMLSATFTKDNVREVDMAEGYAAVKSLELVTDMGLGPAILET 269
           SH +R   G +VRD  G+++ +  F +        AEG+A +++L+   D G+    +E 
Sbjct: 460 SHQNRTGGGALVRDHTGKIIAATAFNRIGQLHPQAAEGWALLEALKWCQDKGININHVEV 519

Query: 270 DSSRVFQILNREREDISELG----------------TFHFVHREGNQAAHRLARLAIAQW 313
           D   +   L    E+++  G                T H   R+GN AAH LA+++I   
Sbjct: 520 DCKNLLTELQTSNENLTSFGNIINAIRRLLSSFPTVTLHHTRRQGNTAAHNLAQVSIG-- 579

[Close]

BLAST of Lag0000212 vs. ExPASy TrEMBL
Match: A0A7J6GT92 (Uncharacterized protein OS=Cannabis sativa OX=3483 GN=F8388_016367 PE=4 SV=1)

HSP 1 Score: 125.2 bits (313), Expect = 6.0e-25
Identity = 81/315 (25.71%), Postives = 139/315 (44.13%), Query Frame = 0


Query: 30  QSGYKLAQSVTLARGASSSSPNSLQDWWKGCWKMTLPSKIKVFLWRL------------- 89
           ++GY +A+        SSS+  +L  WWK  W++ +P KI+ F++RL             
Sbjct: 305 RTGYHVAKQAINRVQPSSSNTETLTRWWKSLWRLPIPPKIRHFVYRLAQHSLPTTNNLYN 364

Query: 90  --CLNRSILREAGFGEILERVRAGCCLLLCQDIKEAIGGEFEELVVLWWSMWSTWNKVRF 149
             C++  I          E V+    L  CQ++K+A   EF   + + W  W+  N   F
Sbjct: 365 RHCISSPICPRCSL--CFESVQH--ALFECQEMKKAWSDEFNLFLCMLWKCWNARNASVF 424

Query: 150 QG-ADRPSGLVEWAKGYVVAFREVGRSSREVGRETVGRERVVWRAPRNGWYKVNFDASFL 209
           +    RP  + + A+ Y+  ++          + T  R+ +VW  P  G+ K+N DA+  
Sbjct: 425 RNQVSRPETIEQEAQDYLAFYQAAQDKRGNHSQSTSDRDLLVWEPPPVGFLKLNTDAAIS 484

Query: 210 SHLSRVDLGIIVRDPLGQVMLSATFTKDNVREVDMAEGYAAVKSLELVTDMGLGPAILET 269
           SH +R   G +VRD  G+++ +  F +        AEG+A +++L+   D G+    +E 
Sbjct: 485 SHQNRTGGGALVRDHTGKIIAATAFNRIGQLHPQAAEGWALLEALKWCQDKGININHVEV 544

Query: 270 DSSRVFQILNREREDISELG----------------TFHFVHREGNQAAHRLARLAIAQW 313
           D   +   L    E+++  G                T H   R+GN AAH LA+++I   
Sbjct: 545 DCKNLLTELQTSNENLTSFGNIINAIRRLLSSFPTVTLHHTRRQGNTAAHNLAQVSIG-- 604

[Close]

BLAST of Lag0000212 vs. ExPASy TrEMBL
Match: A0A7J6F0S9 (Uncharacterized protein OS=Cannabis sativa OX=3483 GN=G4B88_028423 PE=4 SV=1)

HSP 1 Score: 124.4 bits (311), Expect = 1.0e-24
Identity = 80/305 (26.23%), Postives = 137/305 (44.92%), Query Frame = 0


Query: 30  QSGYKLAQSVTLARGASSSSPNSLQDWWKGCWKMTLPSKIKVFLWRL------------- 89
           ++GY +A+        SSS+  +L  WWK  W++ +P KI+ F++RL             
Sbjct: 403 RTGYHVAKQAINRVQPSSSNTETLTRWWKSLWRLPIPPKIRHFVYRLAQHSLPTTNNLYN 462

Query: 90  --CLNRSILREAGFGEILERVRAGCCLLLCQDIKEAIGGEFEELVVLWWSMWSTWNKVRF 149
             C++  I  +       E V+    L  CQ++K+A   EF   + + W  W+  N   F
Sbjct: 463 RHCISSPICPKCSL--CFESVQH--ALFECQEMKKAWSDEFNLFLCMLWKCWNARNASVF 522

Query: 150 QG-ADRPSGLVEWAKGYVVAFREVGRSSREVGRETVGRERVVWRAPRNGWYKVNFDASFL 209
           +    RP  + + A+ Y+  ++          + T  R+ +VW  P  G+ K+N DA+  
Sbjct: 523 RNQVSRPETIEQEAQDYLAFYQAAQDKRWNHSQSTSDRDLLVWEPPPVGFLKLNTDAAIS 582

Query: 210 SHLSRVDLGIIVRDPLGQVMLSATFTKDNVREVDMAEGYAAVKSLELVTDMGLGPAILET 269
           SH +R   G +VRD  G+++ +  F +        AEG+A +++L+   D G+    +E 
Sbjct: 583 SHQNRTGGGALVRDHTGKIIAATAFNRIGQLHPQAAEGWALLEALKWCQDKGININHVEV 642

Query: 270 DSSRVFQILNREREDISELG----------------TFHFVHREGNQAAHRLARLAIAQW 303
           D   +   L    E+++  G                T H   R+GN AAH LA+++I   
Sbjct: 643 DCKNLLTELQTSSENLTSFGNIINAIRRLLSSFPTVTLHHTRRQGNTAAHNLAQMSIG-- 701

[Close]

BLAST of Lag0000212 vs. TAIR 10
Match: AT2G34320.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 55.1 bits (131), Expect = 1.5e-07
Identity = 48/192 (25.00%), Postives = 79/192 (41.15%), Query Frame = 0


Query: 121 LWWSMWSTWNKVRFQGA--DRPSGLVEWAKGYVVAFREVGRSSREVGRETVGRERVVWRA 180
           L W +W + N++ F+G   D P  L    + +           +  G +      V W+A
Sbjct: 80  LLWRLWKSRNELMFKGKEYDAPEVLRRAMEDFEEWSTRRELEGKASGPQVERNLSVQWKA 139

Query: 181 PRNGWYKVNFDASFLSHLSRVDLGIIVRDPLGQVMLSATFTKDNVREVDMAEGYAAVKSL 240
           P   W K N DA++     R  +G I+R+  G V+          + V  AE  A   ++
Sbjct: 140 PPYQWVKCNTDATWQLENPRCGIGWILRNESGGVLWMGARALPRTKNVLEAELEALRWAV 199

Query: 241 ELVTDMGLGPAILETDSSRVFQILNRE---------REDISEL------GTFHFVHREGN 296
             ++       I E+D+  +  +LN +          EDI +L        F F  R GN
Sbjct: 200 LTMSRFNYKRIIFESDAQALVNLLNSDDFWPTLQPALEDIQQLLHHFEEVKFEFTPRGGN 259

The following BLAST results are available for this feature:

BLAST of Lag0000212 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022150918.1	6.7e-39	35.38	uncharacterized protein LOC111018954 [Momordica charantia]	[more]
XP_022140628.1	7.4e-30	44.97	uncharacterized protein LOC111011237 [Momordica charantia]	[more]
KAF4395712.1	1.2e-24	25.71	hypothetical protein G4B88_013486 [Cannabis sativa]	[more]
KAF4386115.1	1.2e-24	25.71	hypothetical protein F8388_016367 [Cannabis sativa]	[more]
KAF4364303.1	2.1e-24	26.23	hypothetical protein G4B88_028423 [Cannabis sativa]	[more]

BLAST of Lag0000212 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Lag0000212 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DAR4	3.3e-39	35.38	uncharacterized protein LOC111018954 OS=Momordica charantia OX=3673 GN=LOC111018...	[more]
A0A6J1CIF1	3.6e-30	44.97	uncharacterized protein LOC111011237 OS=Momordica charantia OX=3673 GN=LOC111011...	[more]
A0A7J6HKE1	6.0e-25	25.71	Uncharacterized protein OS=Cannabis sativa OX=3483 GN=G4B88_013486 PE=4 SV=1	[more]
A0A7J6GT92	6.0e-25	25.71	Uncharacterized protein OS=Cannabis sativa OX=3483 GN=F8388_016367 PE=4 SV=1	[more]
A0A7J6F0S9	1.0e-24	26.23	Uncharacterized protein OS=Cannabis sativa OX=3483 GN=G4B88_028423 PE=4 SV=1	[more]

BLAST of Lag0000212 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G34320.1	1.5e-07	25.00	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002156	Ribonuclease H domain	PFAM	PF13456	RVT_3	coord: 187..267 e-value: 2.8E-9 score: 36.9
IPR036397	Ribonuclease H superfamily	GENE3D	3.30.420.10		coord: 183..295 e-value: 1.3E-7 score: 33.7
None	No IPR available	PANTHER	PTHR47074	BNAC02G40300D PROTEIN	coord: 171..291
None	No IPR available	PANTHER	PTHR47074:SF25	SUBFAMILY NOT NAMED	coord: 171..291
IPR044730	Ribonuclease H-like domain, plant type	CDD	cd06222	RNase_H_like	coord: 186..291 e-value: 1.02459E-10 score: 56.9388
IPR012337	Ribonuclease H-like superfamily	SUPERFAMILY	53098	Ribonuclease H-like	coord: 182..297

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0000212.1	Lag0000212.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0004523	RNA-DNA hybrid ribonuclease activity