Lag0000153 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0000153
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionSHSP domain-containing protein
Locationchr4: 584559 .. 585356 (-)
RNA-Seq ExpressionLag0000153
SyntenyLag0000153
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCTCCATCCACCACCGTCTTCGCCAAAAAAAAACTAATTACACGAGCCATGGCTTCAACTCTTTCCTCTTTACCTTCACCCCTGTTCTTCAAACCCCGATCCCTTATGAAAACCGCACCCAGCCGAGTCTCTATCCCGCCTTTCAGGGTGAGAGCTGAGGCTGATGGCGACCACAGCAAAGACACGTCTTTGGATGTGCATGTCAATCAAGGCGTGGAGAGGCGGCCCGAGCCCAGGAGGCTGGCCCTGGATGTTTCACCTTATGGCAAGTCCTCTCAACACTTAATTAACTCAAAAGCTCATACCCATTTGTCGAACTGAGATTCATTTAGTTACCCTTTTGCAGGAATCTTAGACCCCGTATCTCCAATGAGGACGATGAGGCAAATGCTGGACACAGTGGACAGGCTGTTCGAGTTCGACGACGCAATGCTGAGACCAGGATGGAGGTGGAGCAGAGGAGAGGTGCGAGCCCCTTGGGACATTGAAGAGCACGAAAACCAAATCAGGATGCGTTTCGACATGCCTGGCTTCTCCAAGGAACATGTCAAGGTCTCCATTGAAGACAACTTTTTGACTATAAGAGGCGGCCACGAGAATGCCCAAACCCATGATGACGGCTCCGACTCAGGCTCATGGTCCACAAGGAACGCCTCTACTTACCACACCCGCCTCCAACTTCCTGATGGGATTGACAAGGACAACATCAAGGCCGAGCTCAAGAATGGGGTTCTCTACATTACATTGCCCAAGATCAATGTTCAACGCAACGTCATGGATATTCAGATAGAGTGA

mRNA sequence

ATGTCTCCATCCACCACCGTCTTCGCCAAAAAAAAACTAATTACACGAGCCATGGCTTCAACTCTTTCCTCTTTACCTTCACCCCTGTTCTTCAAACCCCGATCCCTTATGAAAACCGCACCCAGCCGAGTCTCTATCCCGCCTTTCAGGGTGAGAGCTGAGGCTGATGGCGACCACAGCAAAGACACGTCTTTGGATGTGCATGTCAATCAAGGCGTGGAGAGGCGGCCCGAGCCCAGGAGGCTGGCCCTGGATGTTTCACCTTATGGAATCTTAGACCCCGTATCTCCAATGAGGACGATGAGGCAAATGCTGGACACAGTGGACAGGCTGTTCGAGTTCGACGACGCAATGCTGAGACCAGGATGGAGGTGGAGCAGAGGAGAGGTGCGAGCCCCTTGGGACATTGAAGAGCACGAAAACCAAATCAGGATGCGTTTCGACATGCCTGGCTTCTCCAAGGAACATGTCAAGGTCTCCATTGAAGACAACTTTTTGACTATAAGAGGCGGCCACGAGAATGCCCAAACCCATGATGACGGCTCCGACTCAGGCTCATGGTCCACAAGGAACGCCTCTACTTACCACACCCGCCTCCAACTTCCTGATGGGATTGACAAGGACAACATCAAGGCCGAGCTCAAGAATGGGGTTCTCTACATTACATTGCCCAAGATCAATGTTCAACGCAACGTCATGGATATTCAGATAGAGTGA

Coding sequence (CDS)

ATGTCTCCATCCACCACCGTCTTCGCCAAAAAAAAACTAATTACACGAGCCATGGCTTCAACTCTTTCCTCTTTACCTTCACCCCTGTTCTTCAAACCCCGATCCCTTATGAAAACCGCACCCAGCCGAGTCTCTATCCCGCCTTTCAGGGTGAGAGCTGAGGCTGATGGCGACCACAGCAAAGACACGTCTTTGGATGTGCATGTCAATCAAGGCGTGGAGAGGCGGCCCGAGCCCAGGAGGCTGGCCCTGGATGTTTCACCTTATGGAATCTTAGACCCCGTATCTCCAATGAGGACGATGAGGCAAATGCTGGACACAGTGGACAGGCTGTTCGAGTTCGACGACGCAATGCTGAGACCAGGATGGAGGTGGAGCAGAGGAGAGGTGCGAGCCCCTTGGGACATTGAAGAGCACGAAAACCAAATCAGGATGCGTTTCGACATGCCTGGCTTCTCCAAGGAACATGTCAAGGTCTCCATTGAAGACAACTTTTTGACTATAAGAGGCGGCCACGAGAATGCCCAAACCCATGATGACGGCTCCGACTCAGGCTCATGGTCCACAAGGAACGCCTCTACTTACCACACCCGCCTCCAACTTCCTGATGGGATTGACAAGGACAACATCAAGGCCGAGCTCAAGAATGGGGTTCTCTACATTACATTGCCCAAGATCAATGTTCAACGCAACGTCATGGATATTCAGATAGAGTGA

Protein sequence

MSPSTTVFAKKKLITRAMASTLSSLPSPLFFKPRSLMKTAPSRVSIPPFRVRAEADGDHSKDTSLDVHVNQGVERRPEPRRLALDVSPYGILDPVSPMRTMRQMLDTVDRLFEFDDAMLRPGWRWSRGEVRAPWDIEEHENQIRMRFDMPGFSKEHVKVSIEDNFLTIRGGHENAQTHDDGSDSGSWSTRNASTYHTRLQLPDGIDKDNIKAELKNGVLYITLPKINVQRNVMDIQIE
Homology
BLAST of Lag0000153 vs. NCBI nr
Match: ADU55784.1 (HSP25.5 [Citrullus lanatus])

HSP 1 Score: 337.0 bits (863), Expect = 1.3e-88
Identity = 175/229 (76.42%), Postives = 194/229 (84.72%), Query Frame = 0

Query: 18  MASTLSSLPSPLF-FKPRSLMKTAPSRVSIPPFRVRAE--ADGDHSKDTSLDVHVN---Q 77
           MASTLSS PSPL  FKPR L KT P+RV++ P RVRAE  A GDH+KDTSL+VHVN   Q
Sbjct: 1   MASTLSSSPSPLLSFKPRPLKKTPPARVTVLPLRVRAEAGAGGDHNKDTSLEVHVNPSAQ 60

Query: 78  G--VERRPEPRRLALDVSPYGILDPVSPMRTMRQMLDTVDRLFEFDDAMLRPGWRWSRGE 137
           G  VERRP P+RLALDVSPYGILDP+SPMRTMRQMLDTVDRLF+ D  M+RPGWRWSRGE
Sbjct: 61  GTSVERRPGPKRLALDVSPYGILDPLSPMRTMRQMLDTVDRLFD-DAVMMRPGWRWSRGE 120

Query: 138 VRAPWDIEEHENQIRMRFDMPGFSKEHVKVSIEDNFLTIRGGHENAQTHDDGSDSGSWST 197
           VRAPWDIEEHEN+IRMRFDMPG SKEHVKVS+ED+FL I+GGHE   T+   +D G WST
Sbjct: 121 VRAPWDIEEHENEIRMRFDMPGLSKEHVKVSVEDHFLIIKGGHETETTNTSSNDDG-WST 180

Query: 198 RNASTYHTRLQLPDGIDKDNIKAELKNGVLYITLPKINVQRNVMDIQIE 239
           RNAS YHTRLQLP+GID DNIKA+L NGVLYITLPKI VQR VMDI+I+
Sbjct: 181 RNASAYHTRLQLPEGIDTDNIKAQLTNGVLYITLPKIKVQRKVMDIEIQ 227

BLAST of Lag0000153 vs. NCBI nr
Match: XP_038875982.1 (small heat shock protein, chloroplastic [Benincasa hispida])

HSP 1 Score: 328.6 bits (841), Expect = 4.6e-86
Identity = 170/231 (73.59%), Postives = 191/231 (82.68%), Query Frame = 0

Query: 18  MASTLSSLPSPLF-FKPRSLMKTAPSRVSIPPFRVRAE--ADGDHSKDTSLDVHVN---- 77
           MASTLSS PSPL  FKPR L KT P+RV++ P RV+AE  A  DH+KD SL+VHVN    
Sbjct: 1   MASTLSSSPSPLLSFKPRPLKKTPPARVTVLPPRVKAEAGAGSDHNKDASLEVHVNPSGQ 60

Query: 78  -QG--VERRPEPRRLALDVSPYGILDPVSPMRTMRQMLDTVDRLFEFDDAMLRPGWRWSR 137
            QG  VERRP P+RLALDVSPYGILDP+SPMRTMRQMLDTVDRLF+ D  M+ PGWRWSR
Sbjct: 61  GQGTSVERRPGPKRLALDVSPYGILDPLSPMRTMRQMLDTVDRLFD-DAVMMTPGWRWSR 120

Query: 138 GEVRAPWDIEEHENQIRMRFDMPGFSKEHVKVSIEDNFLTIRGGHENAQTHDDGSDSGSW 197
           GEVRAPWDIEEHEN+IRMRFDMPG +KEH KVS+ED+FL I+GGHE   T+   +D G W
Sbjct: 121 GEVRAPWDIEEHENEIRMRFDMPGLTKEHAKVSVEDDFLIIKGGHETETTNSSSNDDG-W 180

Query: 198 STRNASTYHTRLQLPDGIDKDNIKAELKNGVLYITLPKINVQRNVMDIQIE 239
           STRNAS YHTRLQLPDG+DKDNIKA+L NGVLYITLPKI VQR VMDI+I+
Sbjct: 181 STRNASAYHTRLQLPDGVDKDNIKAQLSNGVLYITLPKIKVQRKVMDIEIQ 229

BLAST of Lag0000153 vs. NCBI nr
Match: XP_023551613.1 (small heat shock protein, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 324.7 bits (831), Expect = 6.6e-85
Identity = 170/231 (73.59%), Postives = 190/231 (82.25%), Query Frame = 0

Query: 18  MASTLSSLPSPLF-FKPRSLMKTAPSRVSIPPFRVRAE--ADGDHSKDTSLDVHVN---- 77
           MASTLSS  SPL  FKPR L K  P R++  PFRVRAE  A GDHSKDTSL+VHVN    
Sbjct: 1   MASTLSSSASPLLSFKPRPLKKAPPPRLTSLPFRVRAEAGAGGDHSKDTSLEVHVNPSGQ 60

Query: 78  -QG--VERRPEPRRLALDVSPYGILDPVSPMRTMRQMLDTVDRLFEFDDAMLRPGWRWSR 137
            QG  VERR  P+RLA+DVSPYGI DP+SPMRTMRQMLDTVDRLF+ D AMLRPG  WSR
Sbjct: 61  SQGTSVERRSSPKRLAMDVSPYGIFDPLSPMRTMRQMLDTVDRLFD-DAAMLRPGGVWSR 120

Query: 138 GEVRAPWDIEEHENQIRMRFDMPGFSKEHVKVSIEDNFLTIRGGHENAQTHDDGSDSGSW 197
           GEVRAPWDIEEHEN+I+MRFDMPG SKEHVKVSIEDNFL I+GGHE+  +H++G     W
Sbjct: 121 GEVRAPWDIEEHENEIKMRFDMPGLSKEHVKVSIEDNFLIIKGGHEDTSSHEEG-----W 180

Query: 198 STRNASTYHTRLQLPDGIDKDNIKAELKNGVLYITLPKINVQRNVMDIQIE 239
           ST+NAS YHTRLQLPDG+DKDNIKA+L NGVLYITLPK  +QRNVMDI+I+
Sbjct: 181 STKNASAYHTRLQLPDGVDKDNIKAKLNNGVLYITLPKTKIQRNVMDIEIQ 225

BLAST of Lag0000153 vs. NCBI nr
Match: XP_022922388.1 (small heat shock protein, chloroplastic [Cucurbita moschata])

HSP 1 Score: 322.8 bits (826), Expect = 2.5e-84
Identity = 170/231 (73.59%), Postives = 189/231 (81.82%), Query Frame = 0

Query: 18  MASTLSSLPSPLF-FKPRSLMKTAPSRVSIPPFRVRAE--ADGDHSKDTSLDVHVN---- 77
           MASTLSS  SPL  FKPR L K  P R++  PFRVRAE  A GDHSKDTSL+VHVN    
Sbjct: 1   MASTLSSSASPLLSFKPRPLKKAPPPRLTSLPFRVRAETGAGGDHSKDTSLEVHVNPSGQ 60

Query: 78  -QG--VERRPEPRRLALDVSPYGILDPVSPMRTMRQMLDTVDRLFEFDDAMLRPGWRWSR 137
            QG  VERR  P+RLA+DVSPYGI DP+SPMRTMRQMLDTVDRLF+ D AMLRPG  WSR
Sbjct: 61  SQGTSVERRSSPKRLAMDVSPYGIFDPLSPMRTMRQMLDTVDRLFD-DAAMLRPGGMWSR 120

Query: 138 GEVRAPWDIEEHENQIRMRFDMPGFSKEHVKVSIEDNFLTIRGGHENAQTHDDGSDSGSW 197
           GEVRAPWDIEE EN+I+MRFDMPG SKEHVKVSIEDNFL I+GGHE+  +H++G     W
Sbjct: 121 GEVRAPWDIEEQENEIKMRFDMPGLSKEHVKVSIEDNFLIIKGGHEDTSSHEEG-----W 180

Query: 198 STRNASTYHTRLQLPDGIDKDNIKAELKNGVLYITLPKINVQRNVMDIQIE 239
           ST+NAS YHTRLQLPDGIDKDNIKA+L NGVLYITLPK  +QRNVMDI+I+
Sbjct: 181 STKNASAYHTRLQLPDGIDKDNIKAKLNNGVLYITLPKTKIQRNVMDIEIQ 225

BLAST of Lag0000153 vs. NCBI nr
Match: XP_022974357.1 (small heat shock protein, chloroplastic [Cucurbita maxima])

HSP 1 Score: 322.0 bits (824), Expect = 4.3e-84
Identity = 169/231 (73.16%), Postives = 189/231 (81.82%), Query Frame = 0

Query: 18  MASTLSSLPSPLF-FKPRSLMKTAPSRVSIPPFRVRAE--ADGDHSKDTSLDVHVN---- 77
           MASTLSS  SPL  FKPR L K  P R++  PFRVRAE  A GDHSKDTSL+VHVN    
Sbjct: 1   MASTLSSSASPLLSFKPRPLKKAPPPRLTSLPFRVRAEAGAGGDHSKDTSLEVHVNPSGQ 60

Query: 78  -QG--VERRPEPRRLALDVSPYGILDPVSPMRTMRQMLDTVDRLFEFDDAMLRPGWRWSR 137
            QG  VERRP P+RLA+DVSPYGI DP+SPMRTMRQMLDTVDRLF+ D A+LRPG  WSR
Sbjct: 61  SQGTSVERRPGPKRLAMDVSPYGIFDPLSPMRTMRQMLDTVDRLFD-DAAILRPGGMWSR 120

Query: 138 GEVRAPWDIEEHENQIRMRFDMPGFSKEHVKVSIEDNFLTIRGGHENAQTHDDGSDSGSW 197
           GEVRAPWDIEEHEN+I+MRFDMPG SKEHVKVSIEDNFL I+G HE+  +H++G     W
Sbjct: 121 GEVRAPWDIEEHENEIKMRFDMPGLSKEHVKVSIEDNFLIIKGSHEDTSSHEEG-----W 180

Query: 198 STRNASTYHTRLQLPDGIDKDNIKAELKNGVLYITLPKINVQRNVMDIQIE 239
           S +NAS YHTRLQLPDGIDKDNIKA+L NGVLYITLPK  +QRNVMDI+I+
Sbjct: 181 SAKNASDYHTRLQLPDGIDKDNIKAKLNNGVLYITLPKTKIQRNVMDIEIQ 225

BLAST of Lag0000153 vs. ExPASy Swiss-Prot
Match: P30222 (Small heat shock protein, chloroplastic OS=Petunia hybrida OX=4102 GN=HSP22 PE=2 SV=1)

HSP 1 Score: 213.4 bits (542), Expect = 2.8e-54
Identity = 118/203 (58.13%), Postives = 149/203 (73.40%), Query Frame = 0

Query: 47  PPFRVRAEADGDHSKDTSLDVHV--------NQG--VERRPEPRRLALDVSPYGILDPVS 106
           P  R+ A+A GD +KDTS+DVHV        NQG  VERR  PRR+ALDVSP+G+LDP+S
Sbjct: 49  PASRLVAQATGD-NKDTSVDVHVSNNNQGGNNQGSAVERR--PRRMALDVSPFGLLDPMS 108

Query: 107 PMRTMRQMLDTVDRLFEFDDAMLRPGWR-WSRGEVRAPWDIEEHENQIRMRFDMPGFSKE 166
           PMRTMRQM+DT+DRLFE  D M  PG R    GE+RAPWDI++ EN+I+MRFDMPG SKE
Sbjct: 109 PMRTMRQMMDTMDRLFE--DTMTFPGSRNRGTGEIRAPWDIKDDENEIKMRFDMPGLSKE 168

Query: 167 HVKVSIEDNFLTIRGGHENAQTHDDGSDSGSWSTRNASTYHTRLQLPDGIDKDNIKAELK 226
            VKVS+ED+ L I+G H+  ++  D     SW  RN S+Y TRL LPD +DKD +KAELK
Sbjct: 169 EVKVSVEDDVLVIKGEHKKEESGKD----DSWG-RNYSSYDTRLSLPDNVDKDKVKAELK 228

Query: 227 NGVLYITLPKINVQRNVMDIQIE 239
           NGVL I++PK  V++ V D++I+
Sbjct: 229 NGVLLISIPKTKVEKKVTDVEIK 241

BLAST of Lag0000153 vs. ExPASy Swiss-Prot
Match: P09887 (Small heat shock protein, chloroplastic (Fragment) OS=Glycine max OX=3847 GN=HSP22 PE=2 SV=1)

HSP 1 Score: 212.2 bits (539), Expect = 6.3e-54
Identity = 107/186 (57.53%), Postives = 141/186 (75.81%), Query Frame = 0

Query: 57  GDHSKDTSLDV-HV---NQGVERRPEPRRLALDVSPYGILDPVSPMRTMRQMLDTVDRLF 116
           G  +KD S++V HV   +QG     +PRR A+D+SP+GILDP SPMR+MRQ+LDT+DR+F
Sbjct: 1   GGDNKDNSVEVQHVSKGDQGTAVEKKPRRTAMDISPFGILDPWSPMRSMRQILDTMDRVF 60

Query: 117 EFDDAMLRPGWRWSRGEVRAPWDIEEHENQIRMRFDMPGFSKEHVKVSIEDNFLTIRGGH 176
           E  D M  PG     GE+RAPWDI++ E++IRMRFDMPG +KE VKVS+ED+ L I+GGH
Sbjct: 61  E--DTMTFPGRNIGGGEIRAPWDIKDEEHEIRMRFDMPGLAKEDVKVSVEDDMLVIKGGH 120

Query: 177 ENAQTHDDGSDSGSWSTRNASTYHTRLQLPDGIDKDNIKAELKNGVLYITLPKINVQRNV 236
           ++ Q H  G D  SWS+R  S+Y TRL+LPD  +KD +KAELKNGVLYIT+PK  V+R V
Sbjct: 121 KSEQEH--GGDD-SWSSRTYSSYDTRLKLPDNCEKDKVKAELKNGVLYITIPKTKVERKV 180

Query: 237 MDIQIE 239
           +D+Q++
Sbjct: 181 IDVQVQ 181

BLAST of Lag0000153 vs. ExPASy Swiss-Prot
Match: Q95661 (Small heat shock protein, chloroplastic OS=Solanum lycopersicum OX=4081 GN=HSP21 PE=2 SV=1)

HSP 1 Score: 207.6 bits (527), Expect = 1.5e-52
Identity = 115/213 (53.99%), Postives = 146/213 (68.54%), Query Frame = 0

Query: 39  TAPSRVSIPPF-----RVRAEADGDHSKDTSLDVH-------VNQGVERRPEPRRLALDV 98
           +AP  V +P       R+ A A GD +KDTS+DVH        NQG      P R+ALDV
Sbjct: 29  SAPCSVFVPSMRRPTTRLVARATGD-NKDTSVDVHHSSAQGGNNQGTAVERRPTRMALDV 88

Query: 99  SPYGILDPVSPMRTMRQMLDTVDRLFEFDDAMLRPGWRWS--RGEVRAPWDIEEHENQIR 158
           SP+G+LDP+SPMRTMRQM+DT+DRLFE  D M  PG   +   GE+R PWDI + EN+I+
Sbjct: 89  SPFGVLDPMSPMRTMRQMIDTMDRLFE--DTMTFPGRNRASGTGEIRTPWDIHDDENEIK 148

Query: 159 MRFDMPGFSKEHVKVSIEDNFLTIRGGHENAQTHDDGSDSGSWSTRNASTYHTRLQLPDG 218
           MRFDMPG SKE VKVS+E++ L I+G H+     +DG D  SW  RN S+Y TRL LPD 
Sbjct: 149 MRFDMPGLSKEDVKVSVENDMLVIKGEHKK---EEDGRDKHSWG-RNYSSYDTRLSLPDN 208

Query: 219 IDKDNIKAELKNGVLYITLPKINVQRNVMDIQI 238
           + KD IKAELKNGVL+I++PK  V++ V+D+QI
Sbjct: 209 VVKDKIKAELKNGVLFISIPKTEVEKKVIDVQI 234

BLAST of Lag0000153 vs. ExPASy Swiss-Prot
Match: P31170 (Heat shock protein 21, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=HSP21 PE=1 SV=1)

HSP 1 Score: 204.9 bits (520), Expect = 1.0e-51
Identity = 126/235 (53.62%), Postives = 160/235 (68.09%), Query Frame = 0

Query: 18  MASTL----SSLPSPLFFKPRSLMKTA-PSRVSIP---PFRVRAEADGDHSKDTSLDVHV 77
           MASTL    S+L SPL   P    K+A P  VS P   P R+RA+   ++S D       
Sbjct: 1   MASTLSFAASALCSPLAPSPSVSSKSATPFSVSFPRKIPSRIRAQDQRENSIDVVQQGQQ 60

Query: 78  --NQG--VERRPEPRRLALDVSPYGILDPVSPMRTMRQMLDTVDRLFEFDDAMLRPGWRW 137
             NQG  VE+RP+ +RL +DVSP+G+LDP+SPMRTMRQMLDT+DR+FE D   +    R 
Sbjct: 61  KGNQGSSVEKRPQ-QRLTMDVSPFGLLDPLSPMRTMRQMLDTMDRMFE-DTMPVSGRNRG 120

Query: 138 SRG--EVRAPWDIEEHENQIRMRFDMPGFSKEHVKVSIEDNFLTIRGGHENAQTHDDGSD 197
             G  E+RAPWDI+E E++I+MRFDMPG SKE VK+S+EDN L I+G     Q  +D  D
Sbjct: 121 GSGVSEIRAPWDIKEEEHEIKMRFDMPGLSKEDVKISVEDNVLVIKG----EQKKEDSDD 180

Query: 198 SGSWSTRNASTYHTRLQLPDGIDKDNIKAELKNGVLYITLPKINVQRNVMDIQIE 239
             SWS R+ S+Y TRLQLPD  +KD IKAELKNGVL+IT+PK  V+R V+D+QI+
Sbjct: 181 --SWSGRSVSSYGTRLQLPDNCEKDKIKAELKNGVLFITIPKTKVERKVIDVQIQ 227

BLAST of Lag0000153 vs. ExPASy Swiss-Prot
Match: P09886 (Small heat shock protein, chloroplastic OS=Pisum sativum OX=3888 GN=HSP21 PE=2 SV=1)

HSP 1 Score: 204.1 bits (518), Expect = 1.7e-51
Identity = 114/238 (47.90%), Postives = 160/238 (67.23%), Query Frame = 0

Query: 17  AMASTLSSLPSPLFF-KPRSLMKTAPSRVSIPPFR----------VRAEADGD-HSKDTS 76
           A + +LS++ SP+   KP S +K+ P  ++  P R          VRA+A GD  +KD S
Sbjct: 2   AQSVSLSTIASPILSQKPGSSVKSTPPCMASFPLRRQLPRLGLRNVRAQAGGDGDNKDNS 61

Query: 77  LDVH-VN---QGVERRPEPRRLALDVSPYGILDPVSPMRTMRQMLDTVDRLFEFDDAMLR 136
           ++VH VN   QG     +PRR ++D+SP+G+LDP SPMR+MRQMLDT+DR+FE  DA+  
Sbjct: 62  VEVHRVNKDDQGTAVERKPRRSSIDISPFGLLDPWSPMRSMRQMLDTMDRIFE--DAITI 121

Query: 137 PGWRWSRGEVRAPWDIEEHENQIRMRFDMPGFSKEHVKVSIEDNFLTIRGGHENAQTHDD 196
           PG     GE+R PW+I++ E++IRMRFDMPG SKE VKVS+ED+ L I+  H      +D
Sbjct: 122 PGRNIGGGEIRVPWEIKDEEHEIRMRFDMPGVSKEDVKVSVEDDVLVIKSDHREENGGED 181

Query: 197 GSDSGSWSTRNASTYHTRLQLPDGIDKDNIKAELKNGVLYITLPKINVQRNVMDIQIE 239
                 WS ++ S Y TRL+LPD  +K+ +KAELK+GVLYIT+PK  ++R V+D+QI+
Sbjct: 182 -----CWSRKSYSCYDTRLKLPDNCEKEKVKAELKDGVLYITIPKTKIERTVIDVQIQ 232

BLAST of Lag0000153 vs. ExPASy TrEMBL
Match: H6TB37 (HSP25.5 OS=Citrullus lanatus OX=3654 PE=2 SV=1)

HSP 1 Score: 337.0 bits (863), Expect = 6.2e-89
Identity = 175/229 (76.42%), Postives = 194/229 (84.72%), Query Frame = 0

Query: 18  MASTLSSLPSPLF-FKPRSLMKTAPSRVSIPPFRVRAE--ADGDHSKDTSLDVHVN---Q 77
           MASTLSS PSPL  FKPR L KT P+RV++ P RVRAE  A GDH+KDTSL+VHVN   Q
Sbjct: 1   MASTLSSSPSPLLSFKPRPLKKTPPARVTVLPLRVRAEAGAGGDHNKDTSLEVHVNPSAQ 60

Query: 78  G--VERRPEPRRLALDVSPYGILDPVSPMRTMRQMLDTVDRLFEFDDAMLRPGWRWSRGE 137
           G  VERRP P+RLALDVSPYGILDP+SPMRTMRQMLDTVDRLF+ D  M+RPGWRWSRGE
Sbjct: 61  GTSVERRPGPKRLALDVSPYGILDPLSPMRTMRQMLDTVDRLFD-DAVMMRPGWRWSRGE 120

Query: 138 VRAPWDIEEHENQIRMRFDMPGFSKEHVKVSIEDNFLTIRGGHENAQTHDDGSDSGSWST 197
           VRAPWDIEEHEN+IRMRFDMPG SKEHVKVS+ED+FL I+GGHE   T+   +D G WST
Sbjct: 121 VRAPWDIEEHENEIRMRFDMPGLSKEHVKVSVEDHFLIIKGGHETETTNTSSNDDG-WST 180

Query: 198 RNASTYHTRLQLPDGIDKDNIKAELKNGVLYITLPKINVQRNVMDIQIE 239
           RNAS YHTRLQLP+GID DNIKA+L NGVLYITLPKI VQR VMDI+I+
Sbjct: 181 RNASAYHTRLQLPEGIDTDNIKAQLTNGVLYITLPKIKVQRKVMDIEIQ 227

BLAST of Lag0000153 vs. ExPASy TrEMBL
Match: A0A6J1E6G1 (small heat shock protein, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111430395 PE=3 SV=1)

HSP 1 Score: 322.8 bits (826), Expect = 1.2e-84
Identity = 170/231 (73.59%), Postives = 189/231 (81.82%), Query Frame = 0

Query: 18  MASTLSSLPSPLF-FKPRSLMKTAPSRVSIPPFRVRAE--ADGDHSKDTSLDVHVN---- 77
           MASTLSS  SPL  FKPR L K  P R++  PFRVRAE  A GDHSKDTSL+VHVN    
Sbjct: 1   MASTLSSSASPLLSFKPRPLKKAPPPRLTSLPFRVRAETGAGGDHSKDTSLEVHVNPSGQ 60

Query: 78  -QG--VERRPEPRRLALDVSPYGILDPVSPMRTMRQMLDTVDRLFEFDDAMLRPGWRWSR 137
            QG  VERR  P+RLA+DVSPYGI DP+SPMRTMRQMLDTVDRLF+ D AMLRPG  WSR
Sbjct: 61  SQGTSVERRSSPKRLAMDVSPYGIFDPLSPMRTMRQMLDTVDRLFD-DAAMLRPGGMWSR 120

Query: 138 GEVRAPWDIEEHENQIRMRFDMPGFSKEHVKVSIEDNFLTIRGGHENAQTHDDGSDSGSW 197
           GEVRAPWDIEE EN+I+MRFDMPG SKEHVKVSIEDNFL I+GGHE+  +H++G     W
Sbjct: 121 GEVRAPWDIEEQENEIKMRFDMPGLSKEHVKVSIEDNFLIIKGGHEDTSSHEEG-----W 180

Query: 198 STRNASTYHTRLQLPDGIDKDNIKAELKNGVLYITLPKINVQRNVMDIQIE 239
           ST+NAS YHTRLQLPDGIDKDNIKA+L NGVLYITLPK  +QRNVMDI+I+
Sbjct: 181 STKNASAYHTRLQLPDGIDKDNIKAKLNNGVLYITLPKTKIQRNVMDIEIQ 225

BLAST of Lag0000153 vs. ExPASy TrEMBL
Match: A0A6J1IA25 (small heat shock protein, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111472985 PE=3 SV=1)

HSP 1 Score: 322.0 bits (824), Expect = 2.1e-84
Identity = 169/231 (73.16%), Postives = 189/231 (81.82%), Query Frame = 0

Query: 18  MASTLSSLPSPLF-FKPRSLMKTAPSRVSIPPFRVRAE--ADGDHSKDTSLDVHVN---- 77
           MASTLSS  SPL  FKPR L K  P R++  PFRVRAE  A GDHSKDTSL+VHVN    
Sbjct: 1   MASTLSSSASPLLSFKPRPLKKAPPPRLTSLPFRVRAEAGAGGDHSKDTSLEVHVNPSGQ 60

Query: 78  -QG--VERRPEPRRLALDVSPYGILDPVSPMRTMRQMLDTVDRLFEFDDAMLRPGWRWSR 137
            QG  VERRP P+RLA+DVSPYGI DP+SPMRTMRQMLDTVDRLF+ D A+LRPG  WSR
Sbjct: 61  SQGTSVERRPGPKRLAMDVSPYGIFDPLSPMRTMRQMLDTVDRLFD-DAAILRPGGMWSR 120

Query: 138 GEVRAPWDIEEHENQIRMRFDMPGFSKEHVKVSIEDNFLTIRGGHENAQTHDDGSDSGSW 197
           GEVRAPWDIEEHEN+I+MRFDMPG SKEHVKVSIEDNFL I+G HE+  +H++G     W
Sbjct: 121 GEVRAPWDIEEHENEIKMRFDMPGLSKEHVKVSIEDNFLIIKGSHEDTSSHEEG-----W 180

Query: 198 STRNASTYHTRLQLPDGIDKDNIKAELKNGVLYITLPKINVQRNVMDIQIE 239
           S +NAS YHTRLQLPDGIDKDNIKA+L NGVLYITLPK  +QRNVMDI+I+
Sbjct: 181 SAKNASDYHTRLQLPDGIDKDNIKAKLNNGVLYITLPKTKIQRNVMDIEIQ 225

BLAST of Lag0000153 vs. ExPASy TrEMBL
Match: A0A1S3AV56 (small heat shock protein, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103483006 PE=3 SV=1)

HSP 1 Score: 311.2 bits (796), Expect = 3.7e-81
Identity = 165/231 (71.43%), Postives = 188/231 (81.39%), Query Frame = 0

Query: 18  MASTLSSLPSPLF-FKPRSLMKTAPSRVSIPPFRVRAE--ADGDHSKDTSLDVHVN---- 77
           MASTLS  PSPL  FKPR L K    RV++ P RVRAE  A GD+SKDTSL+VHVN    
Sbjct: 1   MASTLSYSPSPLLSFKPRPLKKIPLPRVTVLPLRVRAEAGASGDNSKDTSLEVHVNPSGQ 60

Query: 78  -QG--VERRPEPRRLALDVSPYGILDPVSPMRTMRQMLDTVDRLFEFDDAMLRPGWRWSR 137
            QG  VERRP P+RLALD+SPYGILDP+SPMRTMRQML+TVDRLF+ D  M+ PG RW+R
Sbjct: 61  GQGTSVERRPGPKRLALDISPYGILDPLSPMRTMRQMLETVDRLFD-DAVMMSPGLRWNR 120

Query: 138 GEVRAPWDIEEHENQIRMRFDMPGFSKEHVKVSIEDNFLTIRGGHENAQTHDDGSDSGSW 197
           G VRAPWDIEEHEN+IRMRFDMPG SKE+VKVS+EDNFL I+GGHE A+T +  S+   W
Sbjct: 121 GGVRAPWDIEEHENEIRMRFDMPGLSKEYVKVSVEDNFLIIKGGHE-AETSNTSSNDDGW 180

Query: 198 STRNASTYHTRLQLPDGIDKDNIKAELKNGVLYITLPKINVQRNVMDIQIE 239
           S RNAS YHTRLQLPDG+DKD+IKA+L NGVLYITLPKI VQR VMDI+I+
Sbjct: 181 SARNASAYHTRLQLPDGVDKDSIKAQLTNGVLYITLPKIKVQRKVMDIEIQ 229

BLAST of Lag0000153 vs. ExPASy TrEMBL
Match: A0A0A0KQ14 (SHSP domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G138480 PE=3 SV=1)

HSP 1 Score: 309.3 bits (791), Expect = 1.4e-80
Identity = 165/231 (71.43%), Postives = 187/231 (80.95%), Query Frame = 0

Query: 18  MASTLSSLPSPLF-FKPRSLMKTAPSRVSIPPFRVRAE--ADGDHSKDTSLDVHVN---- 77
           MASTLSS PS L  FKPR L K  P RV++ P RVRAE  A   +SKDTSL+VHVN    
Sbjct: 1   MASTLSSSPSLLLSFKPRPLKKIPPPRVTVLPLRVRAEAGASSGNSKDTSLEVHVNPSSQ 60

Query: 78  -QG--VERRPEPRRLALDVSPYGILDPVSPMRTMRQMLDTVDRLFEFDDAMLRPGWRWSR 137
            QG  VERRP  +RLALD+SPYGILDP+SPMRTMRQML+TVDRLF+ D  M+ PG RW+R
Sbjct: 61  GQGTSVERRPGSKRLALDISPYGILDPLSPMRTMRQMLETVDRLFD-DAVMMTPGLRWNR 120

Query: 138 GEVRAPWDIEEHENQIRMRFDMPGFSKEHVKVSIEDNFLTIRGGHENAQTHDDGSDSGSW 197
           G VRAPWDIEEHEN+IRMRFDMPG SKE+VKVS+EDNFL I+GGHE A+T +  S+   W
Sbjct: 121 GGVRAPWDIEEHENEIRMRFDMPGLSKEYVKVSVEDNFLIIKGGHE-AETSNTSSNDDGW 180

Query: 198 STRNASTYHTRLQLPDGIDKDNIKAELKNGVLYITLPKINVQRNVMDIQIE 239
           STRNAS YHTRLQLPDG+DKDNIKA+L NGVLYITLPKI VQR VMDI+I+
Sbjct: 181 STRNASAYHTRLQLPDGVDKDNIKAQLTNGVLYITLPKIKVQRKVMDIEIQ 229

BLAST of Lag0000153 vs. TAIR 10
Match: AT4G27670.1 (heat shock protein 21 )

HSP 1 Score: 204.9 bits (520), Expect = 7.1e-53
Identity = 126/235 (53.62%), Postives = 160/235 (68.09%), Query Frame = 0

Query: 18  MASTL----SSLPSPLFFKPRSLMKTA-PSRVSIP---PFRVRAEADGDHSKDTSLDVHV 77
           MASTL    S+L SPL   P    K+A P  VS P   P R+RA+   ++S D       
Sbjct: 1   MASTLSFAASALCSPLAPSPSVSSKSATPFSVSFPRKIPSRIRAQDQRENSIDVVQQGQQ 60

Query: 78  --NQG--VERRPEPRRLALDVSPYGILDPVSPMRTMRQMLDTVDRLFEFDDAMLRPGWRW 137
             NQG  VE+RP+ +RL +DVSP+G+LDP+SPMRTMRQMLDT+DR+FE D   +    R 
Sbjct: 61  KGNQGSSVEKRPQ-QRLTMDVSPFGLLDPLSPMRTMRQMLDTMDRMFE-DTMPVSGRNRG 120

Query: 138 SRG--EVRAPWDIEEHENQIRMRFDMPGFSKEHVKVSIEDNFLTIRGGHENAQTHDDGSD 197
             G  E+RAPWDI+E E++I+MRFDMPG SKE VK+S+EDN L I+G     Q  +D  D
Sbjct: 121 GSGVSEIRAPWDIKEEEHEIKMRFDMPGLSKEDVKISVEDNVLVIKG----EQKKEDSDD 180

Query: 198 SGSWSTRNASTYHTRLQLPDGIDKDNIKAELKNGVLYITLPKINVQRNVMDIQIE 239
             SWS R+ S+Y TRLQLPD  +KD IKAELKNGVL+IT+PK  V+R V+D+QI+
Sbjct: 181 --SWSGRSVSSYGTRLQLPDNCEKDKIKAELKNGVLFITIPKTKVERKVIDVQIQ 227

BLAST of Lag0000153 vs. TAIR 10
Match: AT4G25200.1 (mitochondrion-localized small heat shock protein 23.6 )

HSP 1 Score: 94.4 bits (233), Expect = 1.4e-19
Identity = 77/225 (34.22%), Postives = 116/225 (51.56%), Query Frame = 0

Query: 18  MASTLS---SLPSPLFFKPRSLMKTAPSRVSIPPFRVRAEADGDHSKDTSLDVHVNQGVE 77
           MAS L+    L S +  + RS+++ A S        VR+  D D      +D++      
Sbjct: 1   MASALALKRLLSSSIAPRSRSVLRPAVSSRLFNTNAVRS-YDDDGENGDGVDLY------ 60

Query: 78  RRPEPRRLALDVSPYGILDPVSPMRTMRQMLDTVDRLFEFDDAMLRPGWRWSRGEVRAPW 137
           RR  PRR     S   + DP SP R++ Q+L+ +D+  E  + +L           R  W
Sbjct: 61  RRSVPRRRGDFFS--DVFDPFSPTRSVSQVLNLMDQFME--NPLLSATRGMGASGARRGW 120

Query: 138 DIEEHENQIRMRFDMPGFSKEHVKVSIEDNFLTIRGGHENAQTHDDGSDSGSWSTRNAST 197
           DI+E ++ + +R DMPG S+E VK+++E + L IRG  +N    +DG + G    R    
Sbjct: 121 DIKEKDDALYLRIDMPGLSREDVKLALEQDTLVIRGEGKN---EEDGGEEGESGNRR--- 180

Query: 198 YHTRLQLPDGIDK-DNIKAELKNGVLYITLPKINVQRNVMDIQIE 239
           + +R+ LPD I K D IKAE+KNGVL + +PK+  Q      QIE
Sbjct: 181 FTSRIGLPDKIYKIDEIKAEMKNGVLKVVIPKMKEQERNDVRQIE 208

BLAST of Lag0000153 vs. TAIR 10
Match: AT5G51440.1 (HSP20-like chaperones superfamily protein )

HSP 1 Score: 94.4 bits (233), Expect = 1.4e-19
Identity = 64/197 (32.49%), Postives = 102/197 (51.78%), Query Frame = 0

Query: 51  VRAEADGDHSKDTSLDVHVNQGVERRPEPRRLALDVSPYG------ILDPVSPMRTMRQM 110
           VR  A      +T+   +   GV+R     R    VS +G      ILDP +P R++ QM
Sbjct: 27  VRPVAASSRLFNTNAARNYEDGVDRNHHSNR---HVSRHGGDFFSHILDPFTPTRSLSQM 86

Query: 111 LDTVDRLFEFDDAMLRPGWRWSRGEVRAPWDIEEHENQIRMRFDMPGFSKEHVKVSIEDN 170
           L+ +D++ E        G       VR  W+++E ++ + +R DMPG S+E VK+++E N
Sbjct: 87  LNFMDQVSEIPLVSATRG--MGASGVRRGWNVKEKDDALHLRIDMPGLSREDVKLALEQN 146

Query: 171 FLTIRGGHENAQTHDDGSDSGSWSTRNASTYHTRLQLPDGIDK-DNIKAELKNGVLYITL 230
            L IRG  E  +  D   D           + +R++LP+ + K D IKAE+KNGVL + +
Sbjct: 147 TLVIRGEGETEEGEDVSGD--------GRRFTSRIELPEKVYKTDEIKAEMKNGVLKVVI 206

Query: 231 PKI--NVQRNVMDIQIE 239
           PKI  + + N+  I ++
Sbjct: 207 PKIKEDERNNIRHINVD 210

BLAST of Lag0000153 vs. TAIR 10
Match: AT1G52560.2 (HSP20-like chaperones superfamily protein )

HSP 1 Score: 89.0 bits (219), Expect = 5.7e-18
Identity = 52/196 (26.53%), Postives = 99/196 (50.51%), Query Frame = 0

Query: 50  RVRAEADGDHSKDTSLDVHVN------QGVERRPEPRRLALDVSPYGILDPVSPMRTMRQ 109
           R  A + G+     + +V V+      Q   RR   + L  +   +G   P     T+ Q
Sbjct: 40  RFMATSAGEQEDKMNTEVSVSEKKSPRQNFPRRRGRKSLWRNTDDHGYFTPTLNGNTLIQ 99

Query: 110 MLDTVDRLFEFDDAMLRPGWRWSRGEVRAPWDIEEHENQIRMRFDMPGFSKEHVKVSIED 169
             + ++R+F+           ++    +    ++E ++  ++R+++PG +KE VK+++ D
Sbjct: 100 ATENMNRIFD----------NFNVNPFQLMGQVKEQDDCYKLRYEVPGLTKEDVKITVND 159

Query: 170 NFLTIRGGHENAQTHDDGSDSGSWSTRNASTYHTRLQLPDGIDKDNIKAELKNGVLYITL 229
             LTI+G H+  +      +   WS+++   Y+T L LPD    ++IKAELKNGVL + +
Sbjct: 160 GILTIKGDHKAEEEKGSPEEDEYWSSKSYGYYNTSLSLPDDAKVEDIKAELKNGVLNLVI 219

Query: 230 PKI-NVQRNVMDIQIE 239
           P+    ++NV +I +E
Sbjct: 220 PRTEKPKKNVQEISVE 225

BLAST of Lag0000153 vs. TAIR 10
Match: AT1G52560.1 (HSP20-like chaperones superfamily protein )

HSP 1 Score: 82.4 bits (202), Expect = 5.3e-16
Identity = 37/104 (35.58%), Postives = 67/104 (64.42%), Query Frame = 0

Query: 136 IEEHENQIRMRFDMPGFSKEHVKVSIEDNFLTIRGGHENAQTHDDGSDSGSWSTRNASTY 195
           ++E ++  ++R+++PG +KE VK+++ D  LTI+G H+  +      +   WS+++   Y
Sbjct: 129 VKEQDDCYKLRYEVPGLTKEDVKITVNDGILTIKGDHKAEEEKGSPEEDEYWSSKSYGYY 188

Query: 196 HTRLQLPDGIDKDNIKAELKNGVLYITLPKI-NVQRNVMDIQIE 239
           +T L LPD    ++IKAELKNGVL + +P+    ++NV +I +E
Sbjct: 189 NTSLSLPDDAKVEDIKAELKNGVLNLVIPRTEKPKKNVQEISVE 232

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
ADU55784.11.3e-8876.42HSP25.5 [Citrullus lanatus][more]
XP_038875982.14.6e-8673.59small heat shock protein, chloroplastic [Benincasa hispida][more]
XP_023551613.16.6e-8573.59small heat shock protein, chloroplastic [Cucurbita pepo subsp. pepo][more]
XP_022922388.12.5e-8473.59small heat shock protein, chloroplastic [Cucurbita moschata][more]
XP_022974357.14.3e-8473.16small heat shock protein, chloroplastic [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
P302222.8e-5458.13Small heat shock protein, chloroplastic OS=Petunia hybrida OX=4102 GN=HSP22 PE=2... [more]
P098876.3e-5457.53Small heat shock protein, chloroplastic (Fragment) OS=Glycine max OX=3847 GN=HSP... [more]
Q956611.5e-5253.99Small heat shock protein, chloroplastic OS=Solanum lycopersicum OX=4081 GN=HSP21... [more]
P311701.0e-5153.62Heat shock protein 21, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=HSP21 PE... [more]
P098861.7e-5147.90Small heat shock protein, chloroplastic OS=Pisum sativum OX=3888 GN=HSP21 PE=2 S... [more]
Match NameE-valueIdentityDescription
H6TB376.2e-8976.42HSP25.5 OS=Citrullus lanatus OX=3654 PE=2 SV=1[more]
A0A6J1E6G11.2e-8473.59small heat shock protein, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A6J1IA252.1e-8473.16small heat shock protein, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111472... [more]
A0A1S3AV563.7e-8171.43small heat shock protein, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103483006 ... [more]
A0A0A0KQ141.4e-8071.43SHSP domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G138480 PE=3 S... [more]
Match NameE-valueIdentityDescription
AT4G27670.17.1e-5353.62heat shock protein 21 [more]
AT4G25200.11.4e-1934.22mitochondrion-localized small heat shock protein 23.6 [more]
AT5G51440.11.4e-1932.49HSP20-like chaperones superfamily protein [more]
AT1G52560.25.7e-1826.53HSP20-like chaperones superfamily protein [more]
AT1G52560.15.3e-1635.58HSP20-like chaperones superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002068Alpha crystallin/Hsp20 domainPFAMPF00011HSP20coord: 135..237
e-value: 1.4E-25
score: 89.4
IPR002068Alpha crystallin/Hsp20 domainPROSITEPS01031SHSPcoord: 125..238
score: 24.917858
IPR008978HSP20-like chaperoneGENE3D2.60.40.790coord: 90..238
e-value: 4.1E-29
score: 103.2
IPR008978HSP20-like chaperoneSUPERFAMILY49764HSP20-like chaperonescoord: 96..230
IPR044587Heat shock protein 21-likePANTHERPTHR4673326.5 KDA HEAT SHOCK PROTEIN, MITOCHONDRIALcoord: 20..237
NoneNo IPR availablePANTHERPTHR46733:SF4HEAT SHOCK PROTEIN 21, CHLOROPLASTICcoord: 20..237
NoneNo IPR availableCDDcd06464ACD_sHsps-likecoord: 135..225
e-value: 1.0208E-27
score: 99.5508

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0000153.1Lag0000153.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009408 response to heat