Lag0000140 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0000140
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Cytochrome P450
Location	chr4: 399057 .. 400593 (+)
RNA-Seq Expression	Lag0000140
Synteny	Lag0000140

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCAAGGACATGAACTTTTCACTAGACTACTGGACACTAGCTGCAGGTAGCTCATGTTCACTAATAGCATAAGCTGAGCTGTCATCATTGTTGTCCTTTAAGCATAATCTTGTAAATTATAAAAGATTGATCGTATAGTTCACTTCTCTATCATTATGCCTAGTCTTGTAAATTAAATTAAATAGGTCTCCAAAATATAAAATTAATAACTCAAATTATTTCCTCTAAGAACTCGGAAAATGGTGTCAAGGAATAAGTCATCAACATAGAAAGAAAGCAATTTTTCTCATAAATAGATATGACCAAATAATACCTCCAGAAGGTGTAACTGTTTGTTGGCTGCGGCTATAGTTTCTTTCCCTAGTTGTGCTTTACAGAAGTTCTTGATAACACCAAGAACAACCTCCAATTCAGAAGGATTCTTGGACACCTAAAAATCGGATCAGCAAGGTCAAAATTGTGAATAAGGTCTGGTGCAAGAAAACATGAGGGTCAAACAAGAATACATAATTTTAATTTGGGAAAAATATCTGAAGTTTGAACTTCATGTCCCCTAAAGTTTAAAAAGTGACACTTTAGTCCCCAAAGTTTGAAAAATAGTTCTAAATGGTCCCTGAGTTAATTTGACCGTTAGTTGACTAACTGAAAAATGATGTGGCATGCTGAGGTGACACATTTTAATTGAGGTGACCAATTTAATAATTTTATATTATTACTTTTAGATTACATGGCATTTTATTTCTTTTTTTTCATTTTCTCTTTCTCTCTCTTCCCTTACCTTCCTTCTCCCCTCCGTTCTCCCGTTTCCCTCTCCTCTATCCATGTTCTCCTTCTATTGTCAATGATCTTAGTTCCACTCATTCCTTTCTTCCTTTTCATTTTCACAACCTTTCTGTGGTAGAGAAGGAAACCCACAACCAAAAATCTTCCTCCCAACCCTCTCGCTCTCCCCGTCATCGGCCATCTCCATCTCCTCAAACACCCCATCCACAGAACTCTGCAGACTCTCTCACAAAAGTACAACCCCATTTTCACCCCCTGCCTCGGCACCCGTCTCGTCATCGTCGTCTCCTCCACATCCGCCATCGAATAATGCTTCACCACGAACGACATCGTTTTCGCAAACCGCCCTGAGTTCGTTTCCGGCAAGTACTTCACCTACGACAATAGCACCCTCGGAGCCGCCCCGTACAGCGACCACTGGCGCAACCTGAGGAAGCTGGGCACGACAGAGGTCCTGTCGTCGATCCAACTGAACTCGTCGGCGGGGATCCGAAGGAAGGAAATCGGTATTTTACTGTGGAAGCTGCATCGAATTTCGAGGGACGATTTCGGTAGGGTGAAGTTGAAATCGTTGTTTTCGGAGTTGACGTTCAATATAATCATGAGAATGGTGTCCGAAAAACGGTACTACGGTGAGAAAGTGGTGGAATTGGAAGAGGCGAAGAAGTTTAGAGAAATCATGGAGAAGGCGTTTCAATTGGGGTCATATCCAGGGGATTTCTTGCCGTTTCTGAAGTGGGTGGATCGTTAG

mRNA sequence

ATGGCCAAGGACATGAACTTTTCACTAGACTACTGGACACTAGCTGCAGAACAACCTCCAATTCAGAAGGATTCTTGGACACCTAAAAATCGGATCAGCAAGGTCAAAATTGTGAATAAGTACTTCACCTACGACAATAGCACCCTCGGAGCCGCCCCGTACAGCGACCACTGGCGCAACCTGAGGAAGCTGGGCACGACAGAGGTCCTGTCGTCGATCCAACTGAACTCGTCGGCGGGGATCCGAAGGAAGGAAATCGGTATTTTACTGTGGAAGCTGCATCGAATTTCGAGGGACGATTTCGGTAGGGTGAAGTTGAAATCGTTGTTTTCGGAGTTGACGTTCAATATAATCATGAGAATGGTGTCCGAAAAACGGTACTACGGTGAGAAAGTGGTGGAATTGGAAGAGGCGAAGAAGTTTAGAGAAATCATGGAGAAGGCGTTTCAATTGGGGTCATATCCAGGGGATTTCTTGCCGTTTCTGAAGTGGGTGGATCGTTAG

Coding sequence (CDS)

ATGGCCAAGGACATGAACTTTTCACTAGACTACTGGACACTAGCTGCAGAACAACCTCCAATTCAGAAGGATTCTTGGACACCTAAAAATCGGATCAGCAAGGTCAAAATTGTGAATAAGTACTTCACCTACGACAATAGCACCCTCGGAGCCGCCCCGTACAGCGACCACTGGCGCAACCTGAGGAAGCTGGGCACGACAGAGGTCCTGTCGTCGATCCAACTGAACTCGTCGGCGGGGATCCGAAGGAAGGAAATCGGTATTTTACTGTGGAAGCTGCATCGAATTTCGAGGGACGATTTCGGTAGGGTGAAGTTGAAATCGTTGTTTTCGGAGTTGACGTTCAATATAATCATGAGAATGGTGTCCGAAAAACGGTACTACGGTGAGAAAGTGGTGGAATTGGAAGAGGCGAAGAAGTTTAGAGAAATCATGGAGAAGGCGTTTCAATTGGGGTCATATCCAGGGGATTTCTTGCCGTTTCTGAAGTGGGTGGATCGTTAG

Protein sequence

MAKDMNFSLDYWTLAAEQPPIQKDSWTPKNRISKVKIVNKYFTYDNSTLGAAPYSDHWRNLRKLGTTEVLSSIQLNSSAGIRRKEIGILLWKLHRISRDDFGRVKLKSLFSELTFNIIMRMVSEKRYYGEKVVELEEAKKFREIMEKAFQLGSYPGDFLPFLKWVDR

Homology

[Close]

BLAST of Lag0000140 vs. NCBI nr
Match: KAA0039876.1 (cytochrome P450 81E8-like [Cucumis melo var. makuwa] >TYK24625.1 cytochrome P450 81E8-like [Cucumis melo var. makuwa])

HSP 1 Score: 209.1 bits (531), Expect = 2.8e-50
Identity = 101/130 (77.69%), Postives = 115/130 (88.46%), Query Frame = 0


Query: 37  IVNKYFTYDNSTLGAAPYSDHWRNLRKLGTTEVLSSIQLNSSAGIRRKEIGILLWKLHRI 96
           +  KY TY NSTLGAAPY DHWRNLR+L  TEVLS+I+LN SA IR++EI IL+ KL+R+
Sbjct: 105 VSGKYLTYGNSTLGAAPYGDHWRNLRRLSATEVLSTIRLNMSARIRKEEISILIKKLNRV 164

Query: 97  SRDDFGRVKLKSLFSELTFNIIMRMVSEKRYYGEKVVELEEAKKFREIMEKAFQLGSYPG 156
           S  DFG+VKLKSLFSELTFN+IMRMV+ KRYYGE+V ELEEAKKFREIME+AFQLGSYPG
Sbjct: 165 SSTDFGKVKLKSLFSELTFNMIMRMVAGKRYYGEEVSELEEAKKFREIMERAFQLGSYPG 224

Query: 157 DFLPFLKWVD 167
           DFLPFLKWVD
Sbjct: 225 DFLPFLKWVD 234

[Close]

BLAST of Lag0000140 vs. NCBI nr
Match: XP_008459939.1 (PREDICTED: cytochrome P450 81E8-like [Cucumis melo])

HSP 1 Score: 206.8 bits (525), Expect = 1.4e-49
Identity = 100/130 (76.92%), Postives = 114/130 (87.69%), Query Frame = 0


Query: 37  IVNKYFTYDNSTLGAAPYSDHWRNLRKLGTTEVLSSIQLNSSAGIRRKEIGILLWKLHRI 96
           +  KY TY NSTLGAAPY DHWRNLR+L  TEVLS+I+LN SA IR++EI IL+ KL+R+
Sbjct: 105 VSGKYLTYGNSTLGAAPYGDHWRNLRRLSATEVLSTIRLNMSARIRKEEISILIKKLNRV 164

Query: 97  SRDDFGRVKLKSLFSELTFNIIMRMVSEKRYYGEKVVELEEAKKFREIMEKAFQLGSYPG 156
           S  DFG+VKLKSLFSELTFN+IM MV+ KRYYGE+V ELEEAKKFREIME+AFQLGSYPG
Sbjct: 165 SSTDFGKVKLKSLFSELTFNMIMTMVAGKRYYGEEVSELEEAKKFREIMERAFQLGSYPG 224

Query: 157 DFLPFLKWVD 167
           DFLPFLKWVD
Sbjct: 225 DFLPFLKWVD 234

[Close]

BLAST of Lag0000140 vs. NCBI nr
Match: XP_004140662.1 (cytochrome P450 81Q32 [Cucumis sativus] >AIY67847.1 cytochrome P450 [Cucumis sativus] >KGN46390.1 hypothetical protein Csa_005013 [Cucumis sativus])

HSP 1 Score: 206.8 bits (525), Expect = 1.4e-49
Identity = 99/130 (76.15%), Postives = 115/130 (88.46%), Query Frame = 0


Query: 37  IVNKYFTYDNSTLGAAPYSDHWRNLRKLGTTEVLSSIQLNSSAGIRRKEIGILLWKLHRI 96
           +  KY TY NSTLGAAPY DHWRNLR+L  TE+LS+I+LN SA IR++EIGIL+ KL+R+
Sbjct: 102 VSGKYLTYGNSTLGAAPYGDHWRNLRRLSATEILSTIRLNMSARIRKEEIGILIKKLNRV 161

Query: 97  SRDDFGRVKLKSLFSELTFNIIMRMVSEKRYYGEKVVELEEAKKFREIMEKAFQLGSYPG 156
              +FG+VKLKSLFSELTFNIIMRM++ KRYYGE+V ELEEAKKFREIME+AFQLGSYPG
Sbjct: 162 LGTEFGKVKLKSLFSELTFNIIMRMLAGKRYYGEEVSELEEAKKFREIMERAFQLGSYPG 221

Query: 157 DFLPFLKWVD 167
           DFLPFLKWVD
Sbjct: 222 DFLPFLKWVD 231

[Close]

BLAST of Lag0000140 vs. NCBI nr
Match: XP_023549077.1 (cytochrome P450 81E8-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 203.0 bits (515), Expect = 2.0e-48
Identity = 94/127 (74.02%), Postives = 115/127 (90.55%), Query Frame = 0


Query: 41  YFTYDNSTLGAAPYSDHWRNLRKLGTTEVLSSIQLNSSAGIRRKEIGILLWKLHRISRDD 100
           Y +Y N+T+GAAPY DHWRNLR+L +TE+LSSI+LN SAGIR++E+ ILL KL++ SR D
Sbjct: 107 YLSYGNTTMGAAPYGDHWRNLRRLSSTEILSSIRLNMSAGIRKEEVNILLRKLYQFSRHD 166

Query: 101 FGRVKLKSLFSELTFNIIMRMVSEKRYYGEKVVELEEAKKFREIMEKAFQLGSYPGDFLP 160
           FG+VKLK+LFSELTFNIIMRMV+ KRYYG++V ELEEAK+FR+IME +F+LGSYPGDFLP
Sbjct: 167 FGKVKLKTLFSELTFNIIMRMVAGKRYYGDEVTELEEAKEFRQIMETSFRLGSYPGDFLP 226

Query: 161 FLKWVDR 168
           FLKWVDR
Sbjct: 227 FLKWVDR 233

[Close]

BLAST of Lag0000140 vs. NCBI nr
Match: KAG7014609.1 (Cytochrome P450 81E8, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 203.0 bits (515), Expect = 2.0e-48
Identity = 94/127 (74.02%), Postives = 115/127 (90.55%), Query Frame = 0


Query: 41  YFTYDNSTLGAAPYSDHWRNLRKLGTTEVLSSIQLNSSAGIRRKEIGILLWKLHRISRDD 100
           Y +Y N+T+GAAPY DHWRNLR+L +TE+LSSI+LN SAGIR++E+ ILL KL++ SR D
Sbjct: 107 YLSYGNTTMGAAPYGDHWRNLRRLSSTEILSSIRLNMSAGIRKEEVNILLRKLYQFSRHD 166

Query: 101 FGRVKLKSLFSELTFNIIMRMVSEKRYYGEKVVELEEAKKFREIMEKAFQLGSYPGDFLP 160
           FG+VKLK+LFSELTFNIIMRMV+ KRYYG++V ELEEAK+FR+IME +F+LGSYPGDFLP
Sbjct: 167 FGKVKLKTLFSELTFNIIMRMVAGKRYYGDEVTELEEAKEFRQIMETSFRLGSYPGDFLP 226

Query: 161 FLKWVDR 168
           FLKWVDR
Sbjct: 227 FLKWVDR 233

[Close]

BLAST of Lag0000140 vs. ExPASy Swiss-Prot
Match: Q6WNQ8 (Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1)

HSP 1 Score: 125.6 bits (314), Expect = 5.4e-28
Identity = 67/134 (50.00%), Postives = 92/134 (68.66%), Query Frame = 0


Query: 37  IVNKYFTYDNSTLGAAPYSDHWRNLRKLGTTEVLSSIQLNSSAGIRRKEIGILLWKLHRI 96
           +  KY  Y+N+T+  +PY DHWRNLR++ + E+LSS +LNS   IRR EI  L+ KL + 
Sbjct: 103 LTGKYIGYNNTTVAQSPYGDHWRNLRRILSIEILSSHRLNSFLEIRRDEIMRLIQKLAQK 162

Query: 97  SRDDFGRVKLKSLFSELTFNIIMRMVSEKRYYGE--KVVELEEAKKFREIMEKAFQLG-- 156
           S + F  V+L+ +FSE+TFN IMRMVS KRYYG    V ++EEA+ FR I+++   LG  
Sbjct: 163 SYNGFTEVELRPMFSEMTFNTIMRMVSGKRYYGNDCDVSDVEEARLFRGIIKEVVSLGGA 222

Query: 157 SYPGDFLPFLKWVD 167
           +  GDFL FL+W D
Sbjct: 223 NNVGDFLGFLRWFD 236

[Close]

BLAST of Lag0000140 vs. ExPASy Swiss-Prot
Match: W8JMU7 (Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1)

HSP 1 Score: 125.2 bits (313), Expect = 7.1e-28
Identity = 61/132 (46.21%), Postives = 90/132 (68.18%), Query Frame = 0


Query: 37  IVNKYFTYDNSTLGAAPYSDHWRNLRKLGTTEVLSSIQLNSSAGIRRKEIGILLWKLHRI 96
           I+ KY  Y+ +T+  +PY +HWRNLR+L   E+ S+  LN    IR  E+  LL  L++ 
Sbjct: 104 ILGKYIGYNYTTMVGSPYGEHWRNLRRLAAVEIFSAGSLNRFLSIREDEVKQLLLSLYQS 163

Query: 97  SRDDFGRVKLKSLFSELTFNIIMRMVSEKRYYGEKVVELEEAKKFREIMEKAFQL--GSY 156
           S  DFG+V++KS  SEL+FN+ MRMV+ KRY+G+  V+ +EAK FR ++ + F+    S 
Sbjct: 164 SGQDFGKVEMKSKLSELSFNVTMRMVAGKRYFGQD-VDSDEAKLFRALIGEVFEHAGASN 223

Query: 157 PGDFLPFLKWVD 167
           PGDF+PFL+W+D
Sbjct: 224 PGDFVPFLRWID 234

[Close]

BLAST of Lag0000140 vs. ExPASy Swiss-Prot
Match: Q6WNQ9 (Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 PE=1 SV=1)

HSP 1 Score: 116.7 bits (291), Expect = 2.5e-25
Identity = 64/137 (46.72%), Postives = 93/137 (67.88%), Query Frame = 0


Query: 37  IVNKYFTYDNSTLGAAPYSDHWRNLRKLGTTEVLSSIQLNSSAGIRRKEIGILLWKLHR- 96
           +  KY  Y+ +TLG+A Y DHWRNLR++ T +VLS+ +LNS  G+RR E   L+ KL + 
Sbjct: 101 LTGKYIFYNYTTLGSASYGDHWRNLRRITTIDVLSNNRLNSFLGVRRDETNRLIQKLLKD 160

Query: 97  ISRDDFG--RVKLKSLFSELTFNIIMRMVSEKRYYGE--KVVELEEAKKFREIMEKAFQL 156
           +  + FG  +V+L+   +E+TFN +MRM+S KRYYG+   V ++EEAK+FREI+ +   L
Sbjct: 161 VVSEGFGFTKVELRPRLTEMTFNAMMRMISGKRYYGDDGDVSDVEEAKQFREIISEMMSL 220

Query: 157 --GSYPGDFLPFLKWVD 167
              +  GDFLP L+ VD
Sbjct: 221 LGANNKGDFLPLLRVVD 237

[Close]

BLAST of Lag0000140 vs. ExPASy Swiss-Prot
Match: Q9LVD6 (Cytochrome P450 81F2 OS=Arabidopsis thaliana OX=3702 GN=CYP81F2 PE=2 SV=1)

HSP 1 Score: 109.4 bits (272), Expect = 4.0e-23
Identity = 67/167 (40.12%), Postives = 101/167 (60.48%), Query Frame = 0


Query: 7   FSLDYWT---LAAEQPPIQKDSWTPKNRISKVK----IVNKYFTYDNSTLGAAPYSDHWR 66
           FSL Y +   +     P+ ++S+T +N +        +  KY  YD +T+G A Y DHWR
Sbjct: 65  FSLRYGSRQVVVISSLPLVRESFTGQNDVILTNRPHFLTAKYVAYDYTTIGTAAYGDHWR 124

Query: 67  NLRKLGTTEVLSSIQLNSSAGIRRKEIGILLWKLHRISRDDFGR-VKLKSLFSELTFNII 126
           NLR++ + E+LSS +L     +R+ EI  LL KL   SR+  GR V+L+ L ++LTFN I
Sbjct: 125 NLRRICSLEILSSNRLTGFLSVRKDEIRRLLTKL---SREYDGRVVELEPLLADLTFNNI 184

Query: 127 MRMVSEKRYYGEKVVELEEAKKFREIMEKAFQL--GSYPGDFLPFLK 164
           +RMV+ +RYYG++V   EEA  F++++         S+PGD+LP LK
Sbjct: 185 VRMVTGRRYYGDQVHNKEEANLFKKLVTDINDNSGASHPGDYLPILK 228

[Close]

BLAST of Lag0000140 vs. ExPASy Swiss-Prot
Match: O65790 (Cytochrome P450 81F1 OS=Arabidopsis thaliana OX=3702 GN=CYP81F1 PE=2 SV=2)

HSP 1 Score: 109.0 bits (271), Expect = 5.3e-23
Identity = 62/159 (38.99%), Postives = 101/159 (63.52%), Query Frame = 0


Query: 19  PPIQKDSWTPKNRI----SKVKIVNKYFTYDNSTLGAAPYSDHWRNLRKLGTTEVLSSIQ 78
           P + ++S+T +N I      +++  KY  Y+++T+G APY DHWRNLR++ + E+LSS +
Sbjct: 78  PSLAQESFTGQNDIVLSSRPLQLTAKYVAYNHTTVGTAPYGDHWRNLRRICSQEILSSHR 137

Query: 79  LNSSAGIRRKEIGILLWKLHRISR-----DDFGRVKLKSLFSELTFNIIMRMVSEKRYYG 138
           L +   IR+ EI  +L +L R ++     +DF  ++L+ L S+LTFN I+RMV+ KRYYG
Sbjct: 138 LINFQHIRKDEILRMLTRLSRYTQTSNESNDFTHIELEPLLSDLTFNNIVRMVTGKRYYG 197

Query: 139 EKVVELEEAKKFREIMEKAFQLGSYPG-----DFLPFLK 164
           + V   EEA+ F++++   + +  Y G     D+LP LK
Sbjct: 198 DDVNNKEEAELFKKLV---YDIAMYSGANHSADYLPILK 233

The following BLAST results are available for this feature:

BLAST of Lag0000140 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0039876.1	2.8e-50	77.69	cytochrome P450 81E8-like [Cucumis melo var. makuwa] >TYK24625.1 cytochrome P450...	[more]
XP_008459939.1	1.4e-49	76.92	PREDICTED: cytochrome P450 81E8-like [Cucumis melo]	[more]
XP_004140662.1	1.4e-49	76.15	cytochrome P450 81Q32 [Cucumis sativus] >AIY67847.1 cytochrome P450 [Cucumis sat...	[more]
XP_023549077.1	2.0e-48	74.02	cytochrome P450 81E8-like [Cucurbita pepo subsp. pepo]	[more]
KAG7014609.1	2.0e-48	74.02	Cytochrome P450 81E8, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]

BLAST of Lag0000140 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6WNQ8	5.4e-28	50.00	Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1	[more]
W8JMU7	7.1e-28	46.21	Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1	[more]
Q6WNQ9	2.5e-25	46.72	Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 P...	[more]
Q9LVD6	4.0e-23	40.12	Cytochrome P450 81F2 OS=Arabidopsis thaliana OX=3702 GN=CYP81F2 PE=2 SV=1	[more]
O65790	5.3e-23	38.99	Cytochrome P450 81F1 OS=Arabidopsis thaliana OX=3702 GN=CYP81F1 PE=2 SV=2	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 21..167 e-value: 5.7E-14 score: 53.5
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 40..165
None	No IPR available	PANTHER	PTHR24298	FLAVONOID 3'-MONOOXYGENASE-RELATED	coord: 37..166
None	No IPR available	PANTHER	PTHR24298:SF838	CYTOCHROME P450	coord: 37..166

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0000140.1	Lag0000140.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0016709	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen