Lag0000139 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0000139
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Description1-phosphatidylinositol-3-phosphate 5-kinase
Locationchr4: 396763 .. 398088 (-)
RNA-Seq ExpressionLag0000139
SyntenyLag0000139
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCGGACGATAGTAAAAATTAAACGCTTGATCCTTCAACTTTGCATGAAACGTCCATTACAGTCCAAGGTTCCTATGGATCAAGGTATGCAGTCCTACCTAACCCTTCTTAATTCAATTTACTTTTATGCAGATAGCTTGCATAATGCATTTTTCGGTGCATAAACGATATTGAGGCAACTTTGTTTTGTTTATTTGCATTGCTCGCATGTTTCCCTTCAGGTGTTCCCCATCCTTGTTCAATTCTATTTGTTTATTGCATGCTCATTGTTCTCACAAAGATGCTCCTTTTTTATGCGTCGCTTCTTGAGTTAAGGTCTTAAATTGACGCTCTAGATGTGGCTAGTTGGAGCTTTCTCCTTACAAAAATGTTGGTTCAACGTGCTTGATCTTTCAATCTTGTACGGTGATAGGCCCCAGATAACGAAAGTGTATGAACGAAGGCGCGGGAAGAAGAAAGAGAAGAGAGAGAGTGAGGAGTAGCGAGTTTGTTAGGAGTAGTGGGGCCCGCGTGAATGTCTGTGCGTGGGTAGTAGGGGGTTATAAGGGAATGTTTCGTTAGGGCGTAGGCATCTTTTTGCTATTTTGAATTGTATTTCTCTGATTGAGGAAATTGGGAGAGAGGGGAGCTCTCGAATCTTCCCCGGTTGATGTTGATAAATAAAATTGGTGGCTAAGGCCTATCAATTTTGGTATCAGAGCACTTTGCTGGGGAATGGTGGGAAAGATGGAAGGACGGATCGTTGCGTTGGAAGAGAAAATGTCTGAGTTGGCCGTGAAACAGGTAGATATGGAGGTTAGTTTAGGAACACAGATTGCAGAGGTTGGAGAGGCTCAGGCAAATGTTGACGCCAAATTGGATCTTCAGTTTACAATGGTTAGAGAAGAGATGAAGGCAATGTTTGCTAAGATAGAAGGAATGGTGCATACCGAGAGGGTTCCCCGTCGTCGGAAAGGACCGTTACTGATAGAGGCAAAAGAGTGGTGGAAGAAGAATCGAGCCAGAAACACGAAGAACCCAAAGAAAGCAGTGCGACGTTTTCGAAAGGAAGCGAAGGGATTCAAGCACCAACTAATCGAGATATTCCGCTGTTTGATATGCGATTGAGAAAGCTTGAAGTGCCGGTGTACAAGGGAGAGGAAGGCGAAGACCCAGATGGGTGGTTGCATCGCGTGGAGAGGTACTTTGTGGTTAATCGACTGTCTGAGAAAGACAAGCTCGAAGCGGCGGTGATGTGTTTGGAAGGTGAAGCCTTGTCTTGGCACCAATACGAGGAAGACCGAACACCGATACGTAGCTGGCAGGATTTCGAACGCTGTTGCTAG

mRNA sequence

ATGCGGACGATAGTAAAAATTAAACGCTTGATCCTTCAACTTTGCATGAAACGTCCATTACAGTCCAAGGTTCCTATGGATCAAGGTGTTCCCCATCCTTGTTCAATTCTATTTGTTTATTGCATGCTCATTGTTCTCACAAAGATGCTCCTTTTTTATGCGTCGCTTCTTGAGTTAAGAGCACTTTGCTGGGGAATGGTGGGAAAGATGGAAGGACGGATCGTTGCGTTGGAAGAGAAAATGTCTGAGTTGGCCGTGAAACAGGTAGATATGGAGGTTAGTTTAGGAACACAGATTGCAGAGGTTGGAGAGGCTCAGGCAAATGTTGACGCCAAATTGGATCTTCAGTTTACAATGGGTTCCCCGTCGTCGGAAAGGACCGTTACTGATAGAGGCAAAAGAGTGGTGGAAGAAGAATCGAGCCAGAAACACGAAGAACCCAAAGAAAGCAGTGCGACGTTTTCGAAAGGAAGCGAAGGGATTCAAGCACCAACTAATCGAGATATTCCGCTGTTTGATATGCGATTGAGAAAGCTTGAAGTGCCGGTGTACAAGGGAGAGGAAGGCGAAGACCCAGATGGGTGGTTGCATCGCGTGGAGAGGTACTTTGTGGTTAATCGACTGTCTGAGAAAGACAAGCTCGAAGCGGCGGTGATGTGTTTGGAAGGTGAAGCCTTGTCTTGGCACCAATACGAGGAAGACCGAACACCGATACGTAGCTGGCAGGATTTCGAACGCTGTTGCTAG

Coding sequence (CDS)

ATGCGGACGATAGTAAAAATTAAACGCTTGATCCTTCAACTTTGCATGAAACGTCCATTACAGTCCAAGGTTCCTATGGATCAAGGTGTTCCCCATCCTTGTTCAATTCTATTTGTTTATTGCATGCTCATTGTTCTCACAAAGATGCTCCTTTTTTATGCGTCGCTTCTTGAGTTAAGAGCACTTTGCTGGGGAATGGTGGGAAAGATGGAAGGACGGATCGTTGCGTTGGAAGAGAAAATGTCTGAGTTGGCCGTGAAACAGGTAGATATGGAGGTTAGTTTAGGAACACAGATTGCAGAGGTTGGAGAGGCTCAGGCAAATGTTGACGCCAAATTGGATCTTCAGTTTACAATGGGTTCCCCGTCGTCGGAAAGGACCGTTACTGATAGAGGCAAAAGAGTGGTGGAAGAAGAATCGAGCCAGAAACACGAAGAACCCAAAGAAAGCAGTGCGACGTTTTCGAAAGGAAGCGAAGGGATTCAAGCACCAACTAATCGAGATATTCCGCTGTTTGATATGCGATTGAGAAAGCTTGAAGTGCCGGTGTACAAGGGAGAGGAAGGCGAAGACCCAGATGGGTGGTTGCATCGCGTGGAGAGGTACTTTGTGGTTAATCGACTGTCTGAGAAAGACAAGCTCGAAGCGGCGGTGATGTGTTTGGAAGGTGAAGCCTTGTCTTGGCACCAATACGAGGAAGACCGAACACCGATACGTAGCTGGCAGGATTTCGAACGCTGTTGCTAG

Protein sequence

MRTIVKIKRLILQLCMKRPLQSKVPMDQGVPHPCSILFVYCMLIVLTKMLLFYASLLELRALCWGMVGKMEGRIVALEEKMSELAVKQVDMEVSLGTQIAEVGEAQANVDAKLDLQFTMGSPSSERTVTDRGKRVVEEESSQKHEEPKESSATFSKGSEGIQAPTNRDIPLFDMRLRKLEVPVYKGEEGEDPDGWLHRVERYFVVNRLSEKDKLEAAVMCLEGEALSWHQYEEDRTPIRSWQDFERCC
Homology
BLAST of Lag0000139 vs. NCBI nr
Match: XP_038904464.1 (uncharacterized protein LOC120090832 [Benincasa hispida])

HSP 1 Score: 149.8 bits (377), Expect = 3.0e-32
Identity = 88/203 (43.35%), Postives = 118/203 (58.13%), Query Frame = 0

Query: 66  MVGKMEGRIVALEEKMSELAVKQVDMEVSLGTQIAEVGEAQ-----ANVDA--------- 125
           MV KME R+ A+EE   +L + QV ME  +  +  E+ +       A ++A         
Sbjct: 1   MVKKMEARLSAVEE---QLTLLQVGMENRMDQRFREMQQITESMMIAKIEALGETLEQKM 60

Query: 126 ---------KLDLQFTMGSPSSERTVTDRGKRVVEEESSQKHEEPKESSATFSKGSEGIQ 185
                    KL++     S SSE+TV DRGK+   E+  ++                   
Sbjct: 61  IRPLEVWSKKLEILKEGESGSSEKTVMDRGKQPAREDVGER------------------- 120

Query: 186 APTNRDIPLFDMRLRKLEVPVYKGEEGEDPDGWLHRVERYFVVNRLSEKDKLEAAVMCLE 245
               R++ LFDMRLRKLE+P++KGE GEDP GW HRVERYFVVNRLSEKDK+EAA++CLE
Sbjct: 121 ----REVLLFDMRLRKLEIPIFKGEIGEDPMGWFHRVERYFVVNRLSEKDKIEAAILCLE 177

BLAST of Lag0000139 vs. NCBI nr
Match: TYJ97047.1 (aminoacyl-tRNA ligase [Cucumis melo var. makuwa])

HSP 1 Score: 113.2 bits (282), Expect = 3.2e-21
Identity = 49/71 (69.01%), Postives = 59/71 (83.10%), Query Frame = 0

Query: 174 MRLRKLEVPVYKGEEGEDPDGWLHRVERYFVVNRLSEKDKLEAAVMCLEGEALSWHQYEE 233
           MRLRKLEVP++KGEE +  DGWLHRVERYF+VNRL+EKDKL+ AV+C EGEAL W Q++ 
Sbjct: 1   MRLRKLEVPIFKGEECKSVDGWLHRVERYFMVNRLTEKDKLDVAVLCWEGEALDWFQWKG 60

Query: 234 DRTPIRSWQDF 245
           DR  I SW +F
Sbjct: 61  DRDSIMSWSEF 71

BLAST of Lag0000139 vs. NCBI nr
Match: KAA0039074.1 (keratin, type I cytoskeletal 10-like [Cucumis melo var. makuwa] >TYJ99731.1 keratin, type I cytoskeletal 10-like [Cucumis melo var. makuwa])

HSP 1 Score: 103.2 bits (256), Expect = 3.3e-18
Identity = 56/96 (58.33%), Postives = 71/96 (73.96%), Query Frame = 0

Query: 134 RVVEEESSQKHEEPKESSATFSKGSEGIQ---APTNRDIPLFDMRLRKLEVPVYKGEEGE 193
           R++E E++ + E  + +       +EGI+   AP  RDIPLF MRL KLEVP++KGEE E
Sbjct: 8   RLIELEANLEGESTRMTE--MEVVNEGIRVPCAPNQRDIPLFGMRLWKLEVPIFKGEEEE 67

Query: 194 DPDGWLHRVERYFVVNRLSEKDKLEAAVMCLEGEAL 227
           + D WLH VERYFVVNRL+E+DKLEAAV+CLE EAL
Sbjct: 68  NVDEWLHHVERYFVVNRLTERDKLEAAVLCLEVEAL 101

BLAST of Lag0000139 vs. NCBI nr
Match: XP_022897442.1 (uncharacterized protein LOC111411108 [Olea europaea var. sylvestris])

HSP 1 Score: 99.4 bits (246), Expect = 4.7e-17
Identity = 58/161 (36.02%), Postives = 87/161 (54.04%), Query Frame = 0

Query: 90  DMEVSLGTQIAEVGEAQANVDAKLDLQF----TMGSPSSERTVTDRGKRVVEEESSQKHE 149
           +++  LG+  +E     A +  + ++      T      E+ +T      +  ES     
Sbjct: 67  ELQAGLGSLQSEFRLMHAGMTERFEILMRKWDTQEKERKEKGITSEKSPTLSAESGLTQS 126

Query: 150 EPKESSAT-FSKGSEGIQAPTNRDIPLFDMRLRKLEVPVYKGEEGEDPDGWLHRVERYFV 209
            P  +     S GSE    P          R+R+LE+PV+   EG DPDGW+ RVERYF 
Sbjct: 127 RPSSNQCVGGSAGSEWRGEP----------RIRRLEMPVF---EGNDPDGWVFRVERYFS 186

Query: 210 VNRLSEKDKLEAAVMCLEGEALSWHQYEEDRTPIRSWQDFE 246
           VNRLSE++KLEAA +C +GEAL+W Q+EE R P+++W+D +
Sbjct: 187 VNRLSEEEKLEAAAVCFDGEALAWFQWEERRRPVKAWEDLK 214

BLAST of Lag0000139 vs. NCBI nr
Match: EXB66634.1 (hypothetical protein L484_024931 [Morus notabilis])

HSP 1 Score: 99.0 bits (245), Expect = 6.2e-17
Identity = 41/74 (55.41%), Postives = 60/74 (81.08%), Query Frame = 0

Query: 173 DMRLRKLEVPVYKGEEGEDPDGWLHRVERYFVVNRLSEKDKLEAAVMCLEGEALSWHQYE 232
           D+ LR+LE+P++   EG++P+GWL RVERYF VNRL+E+DKL AA +C +G+AL+W Q+E
Sbjct: 98  DLGLRRLEMPLF---EGDNPEGWLFRVERYFSVNRLTEEDKLSAAAICFKGDALAWFQWE 157

Query: 233 EDRTPIRSWQDFER 247
           + R P+RSW + +R
Sbjct: 158 DGRNPVRSWLELKR 168

BLAST of Lag0000139 vs. ExPASy TrEMBL
Match: A0A5D3BD43 (Aminoacyl-tRNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold506G001130 PE=4 SV=1)

HSP 1 Score: 113.2 bits (282), Expect = 1.5e-21
Identity = 49/71 (69.01%), Postives = 59/71 (83.10%), Query Frame = 0

Query: 174 MRLRKLEVPVYKGEEGEDPDGWLHRVERYFVVNRLSEKDKLEAAVMCLEGEALSWHQYEE 233
           MRLRKLEVP++KGEE +  DGWLHRVERYF+VNRL+EKDKL+ AV+C EGEAL W Q++ 
Sbjct: 1   MRLRKLEVPIFKGEECKSVDGWLHRVERYFMVNRLTEKDKLDVAVLCWEGEALDWFQWKG 60

Query: 234 DRTPIRSWQDF 245
           DR  I SW +F
Sbjct: 61  DRDSIMSWSEF 71

BLAST of Lag0000139 vs. ExPASy TrEMBL
Match: A0A5A7T6F9 (Keratin, type I cytoskeletal 10-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold252G00060 PE=4 SV=1)

HSP 1 Score: 103.2 bits (256), Expect = 1.6e-18
Identity = 56/96 (58.33%), Postives = 71/96 (73.96%), Query Frame = 0

Query: 134 RVVEEESSQKHEEPKESSATFSKGSEGIQ---APTNRDIPLFDMRLRKLEVPVYKGEEGE 193
           R++E E++ + E  + +       +EGI+   AP  RDIPLF MRL KLEVP++KGEE E
Sbjct: 8   RLIELEANLEGESTRMTE--MEVVNEGIRVPCAPNQRDIPLFGMRLWKLEVPIFKGEEEE 67

Query: 194 DPDGWLHRVERYFVVNRLSEKDKLEAAVMCLEGEAL 227
           + D WLH VERYFVVNRL+E+DKLEAAV+CLE EAL
Sbjct: 68  NVDEWLHHVERYFVVNRLTERDKLEAAVLCLEVEAL 101

BLAST of Lag0000139 vs. ExPASy TrEMBL
Match: W9RBJ4 (Retrotrans_gag domain-containing protein OS=Morus notabilis OX=981085 GN=L484_024931 PE=4 SV=1)

HSP 1 Score: 99.0 bits (245), Expect = 3.0e-17
Identity = 41/74 (55.41%), Postives = 60/74 (81.08%), Query Frame = 0

Query: 173 DMRLRKLEVPVYKGEEGEDPDGWLHRVERYFVVNRLSEKDKLEAAVMCLEGEALSWHQYE 232
           D+ LR+LE+P++   EG++P+GWL RVERYF VNRL+E+DKL AA +C +G+AL+W Q+E
Sbjct: 98  DLGLRRLEMPLF---EGDNPEGWLFRVERYFSVNRLTEEDKLSAAAICFKGDALAWFQWE 157

Query: 233 EDRTPIRSWQDFER 247
           + R P+RSW + +R
Sbjct: 158 DGRNPVRSWLELKR 168

BLAST of Lag0000139 vs. ExPASy TrEMBL
Match: A0A2I0WBL0 (1-phosphatidylinositol-3-phosphate 5-kinase OS=Dendrobium catenatum OX=906689 GN=MA16_Dca024607 PE=4 SV=1)

HSP 1 Score: 95.9 bits (237), Expect = 2.5e-16
Identity = 41/75 (54.67%), Postives = 60/75 (80.00%), Query Frame = 0

Query: 173 DMRLRKLEVPVYKGEEGEDPDGWLHRVERYFVVNRLSEKDKLEAAVMCLEGEALSWHQYE 232
           D R+RKL++PV++GE+G    GW+++VERYF VN L E++KL A  MCLEG+AL+W+Q+ 
Sbjct: 13  DPRVRKLKMPVFEGEDGH---GWIYKVERYFAVNGLIEEEKLTAVGMCLEGKALAWYQWC 72

Query: 233 EDRTPIRSWQDFERC 248
           + R PIRSW++F+ C
Sbjct: 73  DRREPIRSWREFKDC 84

BLAST of Lag0000139 vs. ExPASy TrEMBL
Match: A0A2I0VV18 (1-phosphatidylinositol-3-phosphate 5-kinase OS=Dendrobium catenatum OX=906689 GN=MA16_Dca015979 PE=4 SV=1)

HSP 1 Score: 95.5 bits (236), Expect = 3.3e-16
Identity = 38/75 (50.67%), Postives = 61/75 (81.33%), Query Frame = 0

Query: 173 DMRLRKLEVPVYKGEEGEDPDGWLHRVERYFVVNRLSEKDKLEAAVMCLEGEALSWHQYE 232
           D ++RKL++P+++GE+G    GW+++VERYF VN L++ +KL AA +CLEG+AL+W+Q+ 
Sbjct: 13  DPKVRKLKMPIFEGEDGH---GWIYKVERYFAVNGLTDDEKLTAAGLCLEGKALAWYQWR 72

Query: 233 EDRTPIRSWQDFERC 248
           + R PIRSW++F+ C
Sbjct: 73  DRREPIRSWREFKDC 84

BLAST of Lag0000139 vs. TAIR 10
Match: AT1G67020.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: cellular_component unknown; EXPRESSED IN: leaf; Has 72 Blast hits to 72 proteins in 9 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 72; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 57.0 bits (136), Expect = 2.5e-08
Identity = 41/125 (32.80%), Postives = 60/125 (48.00%), Query Frame = 0

Query: 125 ERTVTDRGKRVVEEESSQKHEEPKESSATFSKGSEGIQAPTNRDIPLFDMR---LRKLEV 184
           ER     GKRV + E S +      SS+   +G+   ++P      L D R   +R++E+
Sbjct: 60  ERMEIREGKRVEQGEHSCQSPRHSFSSSMPQRGAG--ESPI-----LLDNRSSLIRRIEM 119

Query: 185 PVYKGEEGEDPDGWLHRVERYFVVNRLSEKDKLEAAVMCLEGEALSWHQYEEDRTPIRSW 244
           PV+   +G     W  +VER+F V R  + DKL+   + LEG AL W   E      R W
Sbjct: 120 PVF---DGSGVYEWFSKVERFFRVGRYQDSDKLDLVALSLEGVALKWFLREMSTLEFRDW 174

Query: 245 QDFER 247
             FE+
Sbjct: 180 NSFEQ 174

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038904464.13.0e-3243.35uncharacterized protein LOC120090832 [Benincasa hispida][more]
TYJ97047.13.2e-2169.01aminoacyl-tRNA ligase [Cucumis melo var. makuwa][more]
KAA0039074.13.3e-1858.33keratin, type I cytoskeletal 10-like [Cucumis melo var. makuwa] >TYJ99731.1 kera... [more]
XP_022897442.14.7e-1736.02uncharacterized protein LOC111411108 [Olea europaea var. sylvestris][more]
EXB66634.16.2e-1755.41hypothetical protein L484_024931 [Morus notabilis][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A5D3BD431.5e-2169.01Aminoacyl-tRNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold50... [more]
A0A5A7T6F91.6e-1858.33Keratin, type I cytoskeletal 10-like OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
W9RBJ43.0e-1755.41Retrotrans_gag domain-containing protein OS=Morus notabilis OX=981085 GN=L484_02... [more]
A0A2I0WBL02.5e-1654.671-phosphatidylinositol-3-phosphate 5-kinase OS=Dendrobium catenatum OX=906689 GN... [more]
A0A2I0VV183.3e-1650.671-phosphatidylinositol-3-phosphate 5-kinase OS=Dendrobium catenatum OX=906689 GN... [more]
Match NameE-valueIdentityDescription
AT1G67020.12.5e-0832.80unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic... [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 127..152
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 120..165

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0000139.1Lag0000139.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016310 phosphorylation
molecular_function GO:0016301 kinase activity
molecular_function GO:0016874 ligase activity