Lag0000138 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0000138
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	E3 ubiquitin-protein ligase SHPRH
Location	chr4: 387118 .. 388627 (-)
RNA-Seq Expression	Lag0000138
Synteny	Lag0000138

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACGCCGAAACCTGCTGTGGACTCGCCGGAAGGGAAGAAGATCACGAGTTTGGTGCTATTGGACTCGCCGGACGATGGTCGGACTGAGGACTACGGAGATGTGGAGAGTTGACGGCGTGAGAGATCGCAGAGGGGAGGGAAGCCGAAGGGTGCTTAATCCTGAATGGAAGAGGGGTTTGAGAGATGGGAAAAATCGAGGGAGAAAGGGTTTTCTTTTTTTAAGTTGCCCTAGTTGTAAGCCAATATTACATAATTGCCCCTCTCAAAATTATAAAGAAAGGTATTCTTGACATTTAAAATATGACATGTAAATTATCAATAAACTTGACATTTTTGAAATGTCAAGTTTTATGTTGGCGTGAAAATTTCCGAAAAATTCCGTCAAGCTCCGTGTTTTTTTTTTTTTCAGTTTTCTTGACATTTTTCATTAAAAAATACATTTTTCTTGACATTTTTCTTAAAAAGAAACATGTTACTTGACATTTTTTCATAAAAACATCAAGTATTTAAATTTTACAAATGTCAAGTAATATGGGTTTTGTAGTAGTGTTTGGAAGGAAAATGGGAAACATGAGGGGTGAAAGAGTCGAAACATGTTTCCTAGATAATAGTCTAGTATCGAGGACGAAGACTAGTGGGGTATATGTCATTATATGAGGACATTATCGTCGAGCACATGCCTCATGATCTTCTTTTTTACCAGTTAATGTATTATCATTGTTTTCCTTCAAACAAAAAACTTAGAGAATAAAACTACATCATATTAGTTTTTGTATAAATGGTGCAAGCTTGTTTCGAAGTCATAATTTTGACTATCTACTACTTTCTGTAGATTGGAGCGGTTGTAAGACGAATCTTGTGGATCAAGTATACAGATTCCAAAGCAAAAGTTCTTGTTTTCTCTAGTTGGAACGATGTTCTTGATGTTTTGCAATATGCCTTTGCCGCGAACGGCATTACCTTCATCAGGATGAAAGGAGGCAGGTGAGATGATATCAAATACAGTGAAAATTTGAATAGCTCAAATCGAAGGCTAGAGCAGAAATCGACCTCTATATTTCAAGTTTCAATTTCAACTGTGTAGCTTACCTTTTCTTCCTCGACCTCGGTTTAGACTTCCATAGCAGGGTTAAACTGCTCTGTACTTCCACGTTATTCAGTAATATTGATGATTTGGCTTTGCAAATGCAGAAAATCTCATACAGCCATTAGTGAATTTAGTGGACAAAAGATCAATGCAAAAGAAAGTCACAAGAACAAGCATCGACACAGGTGCCAGAATTAAGATTTGCTCAAGTTTTATTGTTCTTGATTCAACATGGAGCTAATGGCCTGAACCTCTTAGAAGCTCAACACGTTGTCCTTGTGGAACCACTGTTCAATCCAGCTGCAGAAGCACAAGCAATTAGCAGGGTACACTGAATTGAACAGGAAAACAAGACATTTGTTCACCGATTCATAGTAAGTACGAGTTCCTCTTGTACAGTCATGTAGAGCTTTCACCATAG

mRNA sequence

ATGACGCCGAAACCTGCTGTGGACTCGCCGGAAGGGAAGAAGATCACGAGTTTGGTGCTATTGGACTCGCCGGACGATGGTCGGACTGAGGACTACGGAGATATTGGAGCGGTTGTAAGACGAATCTTGTGGATCAAGTATACAGATTCCAAAGCAAAAGTTCTTGTTTTCTCTAGTTGGAACGATGTTCTTGATGTTTTGCAATATGCCTTTGCCGCGAACGGCATTACCTTCATCAGGATGAAAGGAGGCAGAAAATCTCATACAGCCATTAGTGAATTTAGTGGACAAAAGATCAATGCAAAAGAAAGTCACAAGAACAAGCATCGACACAGTCATGTAGAGCTTTCACCATAG

Coding sequence (CDS)

ATGACGCCGAAACCTGCTGTGGACTCGCCGGAAGGGAAGAAGATCACGAGTTTGGTGCTATTGGACTCGCCGGACGATGGTCGGACTGAGGACTACGGAGATATTGGAGCGGTTGTAAGACGAATCTTGTGGATCAAGTATACAGATTCCAAAGCAAAAGTTCTTGTTTTCTCTAGTTGGAACGATGTTCTTGATGTTTTGCAATATGCCTTTGCCGCGAACGGCATTACCTTCATCAGGATGAAAGGAGGCAGAAAATCTCATACAGCCATTAGTGAATTTAGTGGACAAAAGATCAATGCAAAAGAAAGTCACAAGAACAAGCATCGACACAGTCATGTAGAGCTTTCACCATAG

Protein sequence

MTPKPAVDSPEGKKITSLVLLDSPDDGRTEDYGDIGAVVRRILWIKYTDSKAKVLVFSSWNDVLDVLQYAFAANGITFIRMKGGRKSHTAISEFSGQKINAKESHKNKHRHSHVELSP

Homology

[Close]

BLAST of Lag0000138 vs. NCBI nr
Match: XP_022134046.1 (E3 ubiquitin-protein ligase SHPRH [Momordica charantia] >XP_022134047.1 E3 ubiquitin-protein ligase SHPRH [Momordica charantia])

HSP 1 Score: 136.7 bits (343), Expect = 1.3e-28
Identity = 68/74 (91.89%), Postives = 70/74 (94.59%), Query Frame = 0


Query: 35   IGAVVRRILWIKYTDSKAKVLVFSSWNDVLDVLQYAFAANGITFIRMKGGRKSHTAISEF 94
            I AVVRRILWIKYTDSKAKVLVFSSWNDVLDVLQYAFAANGITFIRMKGGRKS TAISEF
Sbjct: 1450 IEAVVRRILWIKYTDSKAKVLVFSSWNDVLDVLQYAFAANGITFIRMKGGRKSQTAISEF 1509

Query: 95   SGQKINAKESHKNK 109
             GQKINAKE+HK +
Sbjct: 1510 RGQKINAKENHKKR 1523

[Close]

BLAST of Lag0000138 vs. NCBI nr
Match: XP_022938769.1 (E3 ubiquitin-protein ligase SHPRH isoform X9 [Cucurbita moschata] >XP_022938770.1 E3 ubiquitin-protein ligase SHPRH isoform X9 [Cucurbita moschata])

HSP 1 Score: 135.2 bits (339), Expect = 3.7e-28
Identity = 66/74 (89.19%), Postives = 70/74 (94.59%), Query Frame = 0


Query: 35   IGAVVRRILWIKYTDSKAKVLVFSSWNDVLDVLQYAFAANGITFIRMKGGRKSHTAISEF 94
            I AVVRRILWIKYTDS+AKVLVFSSWNDVLDVLQYAFAAN ITF+RMKGGRKSHTAISEF
Sbjct: 1163 IEAVVRRILWIKYTDSEAKVLVFSSWNDVLDVLQYAFAANNITFVRMKGGRKSHTAISEF 1222

Query: 95   SGQKINAKESHKNK 109
             GQKINAKE+HK +
Sbjct: 1223 RGQKINAKENHKKQ 1236

[Close]

BLAST of Lag0000138 vs. NCBI nr
Match: XP_022938762.1 (E3 ubiquitin-protein ligase SHPRH isoform X2 [Cucurbita moschata])

HSP 1 Score: 135.2 bits (339), Expect = 3.7e-28
Identity = 66/74 (89.19%), Postives = 70/74 (94.59%), Query Frame = 0


Query: 35   IGAVVRRILWIKYTDSKAKVLVFSSWNDVLDVLQYAFAANGITFIRMKGGRKSHTAISEF 94
            I AVVRRILWIKYTDS+AKVLVFSSWNDVLDVLQYAFAAN ITF+RMKGGRKSHTAISEF
Sbjct: 1428 IEAVVRRILWIKYTDSEAKVLVFSSWNDVLDVLQYAFAANNITFVRMKGGRKSHTAISEF 1487

Query: 95   SGQKINAKESHKNK 109
             GQKINAKE+HK +
Sbjct: 1488 RGQKINAKENHKKQ 1501

[Close]

BLAST of Lag0000138 vs. NCBI nr
Match: XP_022938767.1 (E3 ubiquitin-protein ligase SHPRH isoform X7 [Cucurbita moschata])

HSP 1 Score: 135.2 bits (339), Expect = 3.7e-28
Identity = 66/74 (89.19%), Postives = 70/74 (94.59%), Query Frame = 0


Query: 35   IGAVVRRILWIKYTDSKAKVLVFSSWNDVLDVLQYAFAANGITFIRMKGGRKSHTAISEF 94
            I AVVRRILWIKYTDS+AKVLVFSSWNDVLDVLQYAFAAN ITF+RMKGGRKSHTAISEF
Sbjct: 1175 IEAVVRRILWIKYTDSEAKVLVFSSWNDVLDVLQYAFAANNITFVRMKGGRKSHTAISEF 1234

Query: 95   SGQKINAKESHKNK 109
             GQKINAKE+HK +
Sbjct: 1235 RGQKINAKENHKKQ 1248

[Close]

BLAST of Lag0000138 vs. NCBI nr
Match: XP_022992999.1 (E3 ubiquitin-protein ligase SHPRH isoform X1 [Cucurbita maxima] >XP_022993000.1 E3 ubiquitin-protein ligase SHPRH isoform X1 [Cucurbita maxima] >XP_022993001.1 E3 ubiquitin-protein ligase SHPRH isoform X1 [Cucurbita maxima])

HSP 1 Score: 135.2 bits (339), Expect = 3.7e-28
Identity = 66/74 (89.19%), Postives = 70/74 (94.59%), Query Frame = 0


Query: 35   IGAVVRRILWIKYTDSKAKVLVFSSWNDVLDVLQYAFAANGITFIRMKGGRKSHTAISEF 94
            I AVVRRILWIKYTDS+AKVLVFSSWNDVLDVLQYAFAAN ITF+RMKGGRKSHTAISEF
Sbjct: 1464 IEAVVRRILWIKYTDSEAKVLVFSSWNDVLDVLQYAFAANNITFVRMKGGRKSHTAISEF 1523

Query: 95   SGQKINAKESHKNK 109
             GQKINAKE+HK +
Sbjct: 1524 RGQKINAKENHKKQ 1537

[Close]

BLAST of Lag0000138 vs. ExPASy Swiss-Prot
Match: Q9UTL9 (Uncharacterized ATP-dependent helicase C144.05 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC144.05 PE=3 SV=1)

HSP 1 Score: 56.2 bits (134), Expect = 2.9e-07
Identity = 28/70 (40.00%), Postives = 43/70 (61.43%), Query Frame = 0


Query: 30   EDYGD-IGAVVRRILWIKYTDSKAKVLVFSSWNDVLDVLQYAFAANGITFIRMKGGRKSH 89
            E +G  I  + + +L++K+ +   KV+VFS W DVLDVL  +F ANGI FIR   G+  +
Sbjct: 1182 ESFGSKIDTISKHLLYLKHNELYPKVVVFSQWLDVLDVLHKSFEANGIVFIRF-DGKSKN 1241

Query: 90   TAISEFSGQK 99
            T +  F  ++
Sbjct: 1242 TCLKRFKEER 1250

[Close]

BLAST of Lag0000138 vs. ExPASy Swiss-Prot
Match: Q149N8 (E3 ubiquitin-protein ligase SHPRH OS=Homo sapiens OX=9606 GN=SHPRH PE=1 SV=2)

HSP 1 Score: 45.4 bits (106), Expect = 5.0e-04
Identity = 22/60 (36.67%), Postives = 34/60 (56.67%), Query Frame = 0


Query: 35   IGAVVRRILWIKYTDSKAKVLVFSSWNDVLDVLQYAFAANGITFIRMKGGRKSHTAISEF 94
            + AVVR ++ I+  D  AK LVFS+W DVLD++  A   N + F ++   +     +S F
Sbjct: 1512 VEAVVRTLMKIQLRDPGAKALVFSTWQDVLDIISKALTDNNMEFAQISRVKTFQENLSAF 1571

[Close]

BLAST of Lag0000138 vs. ExPASy Swiss-Prot
Match: Q7TPQ3 (E3 ubiquitin-protein ligase SHPRH OS=Mus musculus OX=10090 GN=Shprh PE=1 SV=1)

HSP 1 Score: 45.1 bits (105), Expect = 6.6e-04
Identity = 22/60 (36.67%), Postives = 34/60 (56.67%), Query Frame = 0


Query: 35   IGAVVRRILWIKYTDSKAKVLVFSSWNDVLDVLQYAFAANGITFIRMKGGRKSHTAISEF 94
            + AVVR ++ I+  D  AK LVFS+W DVLD++  A   N + F ++   +     +S F
Sbjct: 1503 VEAVVRTLMKIQLRDPGAKALVFSTWQDVLDIISKALTDNNMEFTQISRIKTFQENLSAF 1562

The following BLAST results are available for this feature:

BLAST of Lag0000138 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022134046.1	1.3e-28	91.89	E3 ubiquitin-protein ligase SHPRH [Momordica charantia] >XP_022134047.1 E3 ubiqu...	[more]
XP_022938769.1	3.7e-28	89.19	E3 ubiquitin-protein ligase SHPRH isoform X9 [Cucurbita moschata] >XP_022938770....	[more]
XP_022938762.1	3.7e-28	89.19	E3 ubiquitin-protein ligase SHPRH isoform X2 [Cucurbita moschata]	[more]
XP_022938767.1	3.7e-28	89.19	E3 ubiquitin-protein ligase SHPRH isoform X7 [Cucurbita moschata]	[more]
XP_022992999.1	3.7e-28	89.19	E3 ubiquitin-protein ligase SHPRH isoform X1 [Cucurbita maxima] >XP_022993000.1 ...	[more]

BLAST of Lag0000138 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9UTL9	2.9e-07	40.00	Uncharacterized ATP-dependent helicase C144.05 OS=Schizosaccharomyces pombe (str...	[more]
Q149N8	5.0e-04	36.67	E3 ubiquitin-protein ligase SHPRH OS=Homo sapiens OX=9606 GN=SHPRH PE=1 SV=2	[more]
Q7TPQ3	6.6e-04	36.67	E3 ubiquitin-protein ligase SHPRH OS=Mus musculus OX=10090 GN=Shprh PE=1 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR027417	P-loop containing nucleoside triphosphate hydrolase	GENE3D	3.40.50.300		coord: 26..113 e-value: 1.4E-7 score: 32.9
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 95..118
None	No IPR available	PANTHER	PTHR45865	E3 UBIQUITIN-PROTEIN LIGASE SHPRH FAMILY MEMBER	coord: 35..98

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0000138.1	Lag0000138.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0046872	metal ion binding