Lag0000137 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0000137
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Descriptiontranscription elongation factor TFIIS-like
Locationchr4: 376258 .. 377747 (+)
RNA-Seq ExpressionLag0000137
SyntenyLag0000137
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGAATGAGTTGGTGGAATTGTTTGAAGTAGCGAAGAAGGCTGCAGACGCCGCTGCAGCATCATCAAATGATGGGGGAGTCGAGGAAAGTCGTTGTCTAGATGCCCTACGGCAGCTCAAGAAATTTCCTGTTACTTATCAAATCCTAGTTTCTACTCAGGTTTTCTTCTCATTAGACTTGAAATTTGTTTGTTTTGTTGATGGTGGATTAGTCAAAACGTAAAAAGCCGACTAGTTTGTAAAATCATACATTGTTTTTATTATGAATTAAGCTTTTTTTTTTCCTGGCTTACGAATTGGTTTCATTTCGAGCTGAGTTCACTCTATTTCATCTGTTATTTGAGTTGTTCACATGTCGAAATCATCTCACCTGTGACTCTTTAAAATTTTGGAATATAACTCTGGTTGGCGCCACAGTTGTTATATATTCTGTTCTAGAATTCTTATAATTCCTTTTCTCGTGTAATTGAGGTTCATTTGCCGTTGTTGATTGTTCCGTTATCTTGTCCTCTGTAGGTTGGGAAGCGGCTTCGGCACCTCACTAAACACCCCAGGAGGAAAATACAGGACTATGCTTCTGACCTGATTGAGATGTGGAAGGACATAGTAATCAAGGAGACCAACAAGAATAAGAAAAATGGAAATGGAAATGCCAACTGTAAAGAGCCTCCTAAAATTGGTTCTCCCAGTGCAGAGTCTGTTAAGGTGGAGAAGTTTCAAAAATCATCTTCCATGAAGGTTGAGAAAGTTTCCAAGGTTGAGCACTTCGATTGGAATGGTGCTACAAGCTCTGTAAAATTTTCCAGATCAGAAAGTGCCATCTCAGAGAAAAACTCTGTCAAGGTTGAGAAAACTGATTCAGTTGTCAAGGTTGAAAGAATAGTTAAAGAAGAGAAGAAAATCTCAATAGAAAAGAAACCATCAGGTGGGGCTGCTGCACCTCCAAAGCTTACTTCCATGATTAAATCAAAAGATGCTGCTCGAGACAAAATCAGAGAACTTCTCTTTGAGGCCTTCTCCAAGGTTCCTGGCGAGGCTGGCGAAGATGTTATAGATGAAGTAAATGCAAGTGATCCTATCCGTGTTGCTGTTTCTGTAGAATCCGTGATGTTTGAAAATTGGGGAGGTTCTACCGGAGCACAAAAGGCCAAGTACAGATCTGTAATGTTTAACCTCAAGGATCCGAAGAACCCAGACTTTCGGAGAAAAGTTCTTCTTGGACTTATCAAGCCAGAAAGGCTGATCAACTTGAGCACAACTGATATGGCAAGTGATCAGAGAAAACGTGAGAATGAGGAGATTGCACAAAAGGCTTTATTTGATTGTGAGCGTGGAGGAGCTCCAAAAGCTACTACTGATCAGTTCAAGTGTGGTCGATGTGGTCAACGCAAGACAACCTACTATCAGTTGCAGACGCGGAGTGCTGATGAACCTATGACCACCTTTGTAACTTGTGTAAACTGCAATAACCATTGGAAGTTCTGTTGA

mRNA sequence

ATGGAGAATGAGTTGGTGGAATTGTTTGAAGTAGCGAAGAAGGCTGCAGACGCCGCTGCAGCATCATCAAATGATGGGGGAGTCGAGGAAAGTCGTTGTCTAGATGCCCTACGGCAGCTCAAGAAATTTCCTGTTACTTATCAAATCCTAGTTTCTACTCAGGTTGGGAAGCGGCTTCGGCACCTCACTAAACACCCCAGGAGGAAAATACAGGACTATGCTTCTGACCTGATTGAGATGTGGAAGGACATAGTAATCAAGGAGACCAACAAGAATAAGAAAAATGGAAATGGAAATGCCAACTGTAAAGAGCCTCCTAAAATTGGTTCTCCCAGTGCAGAGTCTGTTAAGGTGGAGAAGTTTCAAAAATCATCTTCCATGAAGGTTGAGAAAGTTTCCAAGGTTGAGCACTTCGATTGGAATGGTGCTACAAGCTCTGTAAAATTTTCCAGATCAGAAAGTGCCATCTCAGAGAAAAACTCTGTCAAGGTTGAGAAAACTGATTCAGTTGTCAAGGTTGAAAGAATAGTTAAAGAAGAGAAGAAAATCTCAATAGAAAAGAAACCATCAGGTGGGGCTGCTGCACCTCCAAAGCTTACTTCCATGATTAAATCAAAAGATGCTGCTCGAGACAAAATCAGAGAACTTCTCTTTGAGGCCTTCTCCAAGGTTCCTGGCGAGGCTGGCGAAGATGTTATAGATGAAGTAAATGCAAGTGATCCTATCCGTGTTGCTGTTTCTGTAGAATCCGTGATGTTTGAAAATTGGGGAGGTTCTACCGGAGCACAAAAGGCCAAGTACAGATCTGTAATGTTTAACCTCAAGGATCCGAAGAACCCAGACTTTCGGAGAAAAGTTCTTCTTGGACTTATCAAGCCAGAAAGGCTGATCAACTTGAGCACAACTGATATGGCAAGTGATCAGAGAAAACGTGAGAATGAGGAGATTGCACAAAAGGCTTTATTTGATTGTGAGCGTGGAGGAGCTCCAAAAGCTACTACTGATCAGTTCAAGTGTGGTCGATGTGGTCAACGCAAGACAACCTACTATCAGTTGCAGACGCGGAGTGCTGATGAACCTATGACCACCTTTGTAACTTGTGTAAACTGCAATAACCATTGGAAGTTCTGTTGA

Coding sequence (CDS)

ATGGAGAATGAGTTGGTGGAATTGTTTGAAGTAGCGAAGAAGGCTGCAGACGCCGCTGCAGCATCATCAAATGATGGGGGAGTCGAGGAAAGTCGTTGTCTAGATGCCCTACGGCAGCTCAAGAAATTTCCTGTTACTTATCAAATCCTAGTTTCTACTCAGGTTGGGAAGCGGCTTCGGCACCTCACTAAACACCCCAGGAGGAAAATACAGGACTATGCTTCTGACCTGATTGAGATGTGGAAGGACATAGTAATCAAGGAGACCAACAAGAATAAGAAAAATGGAAATGGAAATGCCAACTGTAAAGAGCCTCCTAAAATTGGTTCTCCCAGTGCAGAGTCTGTTAAGGTGGAGAAGTTTCAAAAATCATCTTCCATGAAGGTTGAGAAAGTTTCCAAGGTTGAGCACTTCGATTGGAATGGTGCTACAAGCTCTGTAAAATTTTCCAGATCAGAAAGTGCCATCTCAGAGAAAAACTCTGTCAAGGTTGAGAAAACTGATTCAGTTGTCAAGGTTGAAAGAATAGTTAAAGAAGAGAAGAAAATCTCAATAGAAAAGAAACCATCAGGTGGGGCTGCTGCACCTCCAAAGCTTACTTCCATGATTAAATCAAAAGATGCTGCTCGAGACAAAATCAGAGAACTTCTCTTTGAGGCCTTCTCCAAGGTTCCTGGCGAGGCTGGCGAAGATGTTATAGATGAAGTAAATGCAAGTGATCCTATCCGTGTTGCTGTTTCTGTAGAATCCGTGATGTTTGAAAATTGGGGAGGTTCTACCGGAGCACAAAAGGCCAAGTACAGATCTGTAATGTTTAACCTCAAGGATCCGAAGAACCCAGACTTTCGGAGAAAAGTTCTTCTTGGACTTATCAAGCCAGAAAGGCTGATCAACTTGAGCACAACTGATATGGCAAGTGATCAGAGAAAACGTGAGAATGAGGAGATTGCACAAAAGGCTTTATTTGATTGTGAGCGTGGAGGAGCTCCAAAAGCTACTACTGATCAGTTCAAGTGTGGTCGATGTGGTCAACGCAAGACAACCTACTATCAGTTGCAGACGCGGAGTGCTGATGAACCTATGACCACCTTTGTAACTTGTGTAAACTGCAATAACCATTGGAAGTTCTGTTGA

Protein sequence

MENELVELFEVAKKAADAAAASSNDGGVEESRCLDALRQLKKFPVTYQILVSTQVGKRLRHLTKHPRRKIQDYASDLIEMWKDIVIKETNKNKKNGNGNANCKEPPKIGSPSAESVKVEKFQKSSSMKVEKVSKVEHFDWNGATSSVKFSRSESAISEKNSVKVEKTDSVVKVERIVKEEKKISIEKKPSGGAAAPPKLTSMIKSKDAARDKIRELLFEAFSKVPGEAGEDVIDEVNASDPIRVAVSVESVMFENWGGSTGAQKAKYRSVMFNLKDPKNPDFRRKVLLGLIKPERLINLSTTDMASDQRKRENEEIAQKALFDCERGGAPKATTDQFKCGRCGQRKTTYYQLQTRSADEPMTTFVTCVNCNNHWKFC
Homology
BLAST of Lag0000137 vs. NCBI nr
Match: XP_038884917.1 (transcription elongation factor TFIIS [Benincasa hispida])

HSP 1 Score: 670.6 bits (1729), Expect = 7.8e-189
Identity = 343/377 (90.98%), Postives = 361/377 (95.76%), Query Frame = 0

Query: 1   MENELVELFEVAKKAADAAAASSNDGGVEESRCLDALRQLKKFPVTYQILVSTQVGKRLR 60
           MENELVELF+ AKKAADAAAA SNDGG EESRCLDALRQLKKFPVTYQILVSTQVGKRLR
Sbjct: 1   MENELVELFQAAKKAADAAAAPSNDGGAEESRCLDALRQLKKFPVTYQILVSTQVGKRLR 60

Query: 61  HLTKHPRRKIQDYASDLIEMWKDIVIKETNKNKKNGNGNANCKEPPKIGSPSAESVKVEK 120
           HLTKHP++KIQ+YASDL+EMWK+IVIKETNKNKKNGNG  NCK+ PKIGSPSAESVKVEK
Sbjct: 61  HLTKHPKKKIQEYASDLVEMWKEIVIKETNKNKKNGNG--NCKDSPKIGSPSAESVKVEK 120

Query: 121 FQKSSSMKVEKVSKVEHFDWNGATSSVKFSRSESAISEKNSVKVEKTDSVVKVERIVKEE 180
           FQKSSSMKVE+VSKVE FD NGAT SVK+SR ESA+SE+NSVKVEKTDS VKVE  VKEE
Sbjct: 121 FQKSSSMKVERVSKVEQFDKNGATGSVKYSRLESAVSERNSVKVEKTDSAVKVEGTVKEE 180

Query: 181 KKISIEKKPSGGAAAPPKLTSMIKSKDAARDKIRELLFEAFSKVPGEAGEDVIDEVNASD 240
           ++ISI+KKPSGG AAPPKLTSMIKSKDAARDKIRELLFEAFSKVPGEA EDV+DEVNASD
Sbjct: 181 RRISIDKKPSGGTAAPPKLTSMIKSKDAARDKIRELLFEAFSKVPGEADEDVMDEVNASD 240

Query: 241 PIRVAVSVESVMFENWGGSTGAQKAKYRSVMFNLKDPKNPDFRRKVLLGLIKPERLINLS 300
           PIRVAVSVESVMFENWGGSTGAQKAKYRS+MFNLKDPKNPDFRRKVLLGLIKPER+IN+S
Sbjct: 241 PIRVAVSVESVMFENWGGSTGAQKAKYRSIMFNLKDPKNPDFRRKVLLGLIKPERMINMS 300

Query: 301 TTDMASDQRKRENEEIAQKALFDCERGGAPKATTDQFKCGRCGQRKTTYYQLQTRSADEP 360
           T DMASDQRKRENEEIAQKALFDCERGGAPKATTDQFKCGRCGQRKTTYYQLQTRSADEP
Sbjct: 301 TADMASDQRKRENEEIAQKALFDCERGGAPKATTDQFKCGRCGQRKTTYYQLQTRSADEP 360

Query: 361 MTTFVTCVNCNNHWKFC 378
           MTTFVTCVNCNNHWKFC
Sbjct: 361 MTTFVTCVNCNNHWKFC 375

BLAST of Lag0000137 vs. NCBI nr
Match: XP_022957122.1 (transcription elongation factor TFIIS-like [Cucurbita moschata])

HSP 1 Score: 668.7 bits (1724), Expect = 3.0e-188
Identity = 350/377 (92.84%), Postives = 360/377 (95.49%), Query Frame = 0

Query: 1   MENELVELFEVAKKAADAAAASSNDGGVEESRCLDALRQLKKFPVTYQILVSTQVGKRLR 60
           ME ELVELFE AKKAADAAAA SNDGG EESRCLDALRQLKKFPVTYQILVSTQVGKRLR
Sbjct: 1   MEKELVELFEAAKKAADAAAARSNDGGAEESRCLDALRQLKKFPVTYQILVSTQVGKRLR 60

Query: 61  HLTKHPRRKIQDYASDLIEMWKDIVIKETNKNKKNGNGNANCKEPPKIGSPSAESVKVEK 120
           HLTKHP++KIQDYASDLIEMWKDIVIKETNKNKKNGNGN+  KEP KIGSPSAESVKVE 
Sbjct: 61  HLTKHPKKKIQDYASDLIEMWKDIVIKETNKNKKNGNGNS--KEPSKIGSPSAESVKVEN 120

Query: 121 FQKSSSMKVEKVSKVEHFDWNGATSSVKFSRSESAISEKNSVKVEKTDSVVKVERIVKEE 180
            QKSSSMKVE+VSKVE FD NG+TSSVKFSRS SA+SEKNSVKVEKTDSVVKVER+VKEE
Sbjct: 121 LQKSSSMKVERVSKVEQFDRNGSTSSVKFSRSASAVSEKNSVKVEKTDSVVKVERMVKEE 180

Query: 181 KKISIEKKPSGGAAAPPKLTSMIKSKDAARDKIRELLFEAFSKVPGEAGEDVIDEVNASD 240
           KKISIEKKPS GAA PPKLTSMIKSKDAARDKIRELL EAFSKVPGEA EDVIDEVNASD
Sbjct: 181 KKISIEKKPS-GAAGPPKLTSMIKSKDAARDKIRELLVEAFSKVPGEADEDVIDEVNASD 240

Query: 241 PIRVAVSVESVMFENWGGSTGAQKAKYRSVMFNLKDPKNPDFRRKVLLGLIKPERLINLS 300
           PIRVAVSVESVMFENWGGSTGAQKAKYRS+MFNLKDPKNPDFRRKVLLGLIKPER+ NLS
Sbjct: 241 PIRVAVSVESVMFENWGGSTGAQKAKYRSIMFNLKDPKNPDFRRKVLLGLIKPERMTNLS 300

Query: 301 TTDMASDQRKRENEEIAQKALFDCERGGAPKATTDQFKCGRCGQRKTTYYQLQTRSADEP 360
           TTDMASDQRKRENEEIAQKALF+CERGGAPKATTDQFKCGRCGQRKTTYYQLQTRSADEP
Sbjct: 301 TTDMASDQRKRENEEIAQKALFECERGGAPKATTDQFKCGRCGQRKTTYYQLQTRSADEP 360

Query: 361 MTTFVTCVNCNNHWKFC 378
           MTTFVTCVNCNNHWKFC
Sbjct: 361 MTTFVTCVNCNNHWKFC 374

BLAST of Lag0000137 vs. NCBI nr
Match: KAG6602582.1 (Transcription elongation factor TFIIS, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 667.5 bits (1721), Expect = 6.6e-188
Identity = 349/377 (92.57%), Postives = 360/377 (95.49%), Query Frame = 0

Query: 1   MENELVELFEVAKKAADAAAASSNDGGVEESRCLDALRQLKKFPVTYQILVSTQVGKRLR 60
           ME ELVELFE AKKAADAAAA SNDGG EESRCLDALRQLKKFPVTYQILVSTQVGKRLR
Sbjct: 300 MEKELVELFEAAKKAADAAAARSNDGGAEESRCLDALRQLKKFPVTYQILVSTQVGKRLR 359

Query: 61  HLTKHPRRKIQDYASDLIEMWKDIVIKETNKNKKNGNGNANCKEPPKIGSPSAESVKVEK 120
           HLTKHP++KIQDYASDLIEMWKDIVIKETNKNKKNGNGN+  KEP KIGSPSAESVKVE 
Sbjct: 360 HLTKHPKKKIQDYASDLIEMWKDIVIKETNKNKKNGNGNS--KEPSKIGSPSAESVKVEN 419

Query: 121 FQKSSSMKVEKVSKVEHFDWNGATSSVKFSRSESAISEKNSVKVEKTDSVVKVERIVKEE 180
            QKSSSMKVE+VSKVE FD NG+TSSVKFSRS SA+SEKNSVKVEKTDSVVKVERIVKEE
Sbjct: 420 LQKSSSMKVERVSKVEQFDRNGSTSSVKFSRSASAVSEKNSVKVEKTDSVVKVERIVKEE 479

Query: 181 KKISIEKKPSGGAAAPPKLTSMIKSKDAARDKIRELLFEAFSKVPGEAGEDVIDEVNASD 240
           KKISIEKKPS GAA PPKLT+MIKSKDAARDKIRELL EAFSKVPGEA EDV+DEVNASD
Sbjct: 480 KKISIEKKPS-GAAGPPKLTAMIKSKDAARDKIRELLVEAFSKVPGEADEDVMDEVNASD 539

Query: 241 PIRVAVSVESVMFENWGGSTGAQKAKYRSVMFNLKDPKNPDFRRKVLLGLIKPERLINLS 300
           PIRVAVSVESVMFENWGGSTGAQKAKYRS+MFNLKDPKNPDFRRKVLLGLIKPER+ NLS
Sbjct: 540 PIRVAVSVESVMFENWGGSTGAQKAKYRSIMFNLKDPKNPDFRRKVLLGLIKPERMTNLS 599

Query: 301 TTDMASDQRKRENEEIAQKALFDCERGGAPKATTDQFKCGRCGQRKTTYYQLQTRSADEP 360
           TTDMASDQRKRENEEIAQKALF+CERGGAPKATTDQFKCGRCGQRKTTYYQLQTRSADEP
Sbjct: 600 TTDMASDQRKRENEEIAQKALFECERGGAPKATTDQFKCGRCGQRKTTYYQLQTRSADEP 659

Query: 361 MTTFVTCVNCNNHWKFC 378
           MTTFVTCVNCNNHWKFC
Sbjct: 660 MTTFVTCVNCNNHWKFC 673

BLAST of Lag0000137 vs. NCBI nr
Match: XP_022989946.1 (transcription elongation factor TFIIS-like [Cucurbita maxima])

HSP 1 Score: 665.6 bits (1716), Expect = 2.5e-187
Identity = 349/377 (92.57%), Postives = 360/377 (95.49%), Query Frame = 0

Query: 1   MENELVELFEVAKKAADAAAASSNDGGVEESRCLDALRQLKKFPVTYQILVSTQVGKRLR 60
           ME ELVELFE AKKAADAAAA SNDGG EESRCLDALRQLKKFPVTYQILVSTQVGKRLR
Sbjct: 1   MEKELVELFEAAKKAADAAAAPSNDGGAEESRCLDALRQLKKFPVTYQILVSTQVGKRLR 60

Query: 61  HLTKHPRRKIQDYASDLIEMWKDIVIKETNKNKKNGNGNANCKEPPKIGSPSAESVKVEK 120
           HLTKHP++KIQDYASDLIEMWKDIVIKETNKNKKNGNGN+  KEP KIGSPSAESVKVE 
Sbjct: 61  HLTKHPKKKIQDYASDLIEMWKDIVIKETNKNKKNGNGNS--KEPSKIGSPSAESVKVEN 120

Query: 121 FQKSSSMKVEKVSKVEHFDWNGATSSVKFSRSESAISEKNSVKVEKTDSVVKVERIVKEE 180
           FQKSSSMKVE+VSKVE F+  G+TSSVKFSRS SA+SEKNSVKVEKTDSVVKVER+VKEE
Sbjct: 121 FQKSSSMKVERVSKVEQFNRIGSTSSVKFSRSASAVSEKNSVKVEKTDSVVKVERMVKEE 180

Query: 181 KKISIEKKPSGGAAAPPKLTSMIKSKDAARDKIRELLFEAFSKVPGEAGEDVIDEVNASD 240
           KKISIEKKPS GAA PPKLTSMIKSKDAARDKIRELL EAFSKVPGEA EDVIDEVNASD
Sbjct: 181 KKISIEKKPS-GAAGPPKLTSMIKSKDAARDKIRELLVEAFSKVPGEADEDVIDEVNASD 240

Query: 241 PIRVAVSVESVMFENWGGSTGAQKAKYRSVMFNLKDPKNPDFRRKVLLGLIKPERLINLS 300
           PIRVAVSVESVMFENWGGSTGAQKAKYRS+MFNLKDPKNPDFRRKVLLGLIKPER+ NLS
Sbjct: 241 PIRVAVSVESVMFENWGGSTGAQKAKYRSIMFNLKDPKNPDFRRKVLLGLIKPERMTNLS 300

Query: 301 TTDMASDQRKRENEEIAQKALFDCERGGAPKATTDQFKCGRCGQRKTTYYQLQTRSADEP 360
           TTDMASDQRKRENEEIAQKALF+CERGGAPKATTDQFKCGRCGQRKTTYYQLQTRSADEP
Sbjct: 301 TTDMASDQRKRENEEIAQKALFECERGGAPKATTDQFKCGRCGQRKTTYYQLQTRSADEP 360

Query: 361 MTTFVTCVNCNNHWKFC 378
           MTTFVTCVNCNNHWKFC
Sbjct: 361 MTTFVTCVNCNNHWKFC 374

BLAST of Lag0000137 vs. NCBI nr
Match: KAG7033260.1 (Transcription elongation factor TFIIS, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 665.2 bits (1715), Expect = 3.3e-187
Identity = 348/377 (92.31%), Postives = 359/377 (95.23%), Query Frame = 0

Query: 1   MENELVELFEVAKKAADAAAASSNDGGVEESRCLDALRQLKKFPVTYQILVSTQVGKRLR 60
           ME ELVELFE AKKAADAAAA SNDGG EESRCLDALRQLKKFPVTYQILVSTQVGKRLR
Sbjct: 1   MEKELVELFEAAKKAADAAAARSNDGGAEESRCLDALRQLKKFPVTYQILVSTQVGKRLR 60

Query: 61  HLTKHPRRKIQDYASDLIEMWKDIVIKETNKNKKNGNGNANCKEPPKIGSPSAESVKVEK 120
           HLTKHP++KIQDYASDLIEMWKDIVIKETNKNKKNGNG++  KEP KIGSPSAESVKVE 
Sbjct: 61  HLTKHPKKKIQDYASDLIEMWKDIVIKETNKNKKNGNGDS--KEPSKIGSPSAESVKVEN 120

Query: 121 FQKSSSMKVEKVSKVEHFDWNGATSSVKFSRSESAISEKNSVKVEKTDSVVKVERIVKEE 180
            QKSSSMK E+VSKVE FD NG+TSSVKFSRS SA+SEKNSVKVEKTDSVVKVERIVKEE
Sbjct: 121 LQKSSSMKAERVSKVEQFDRNGSTSSVKFSRSASAVSEKNSVKVEKTDSVVKVERIVKEE 180

Query: 181 KKISIEKKPSGGAAAPPKLTSMIKSKDAARDKIRELLFEAFSKVPGEAGEDVIDEVNASD 240
           KKISIEKKPS GAA PPKLTSMIKSKDAARDKIRELL EAFSKVPGEA EDV+DEVNASD
Sbjct: 181 KKISIEKKPS-GAAGPPKLTSMIKSKDAARDKIRELLVEAFSKVPGEADEDVMDEVNASD 240

Query: 241 PIRVAVSVESVMFENWGGSTGAQKAKYRSVMFNLKDPKNPDFRRKVLLGLIKPERLINLS 300
           PIRVAVSVESVMFENWGGSTGAQKAKYRS+MFNLKDPKNPDFRRKVLLGLIKPER+ NLS
Sbjct: 241 PIRVAVSVESVMFENWGGSTGAQKAKYRSIMFNLKDPKNPDFRRKVLLGLIKPERMTNLS 300

Query: 301 TTDMASDQRKRENEEIAQKALFDCERGGAPKATTDQFKCGRCGQRKTTYYQLQTRSADEP 360
           TTDMASDQRKRENEEIAQKALF+CERGGAPKATTDQFKCGRCGQRKTTYYQLQTRSADEP
Sbjct: 301 TTDMASDQRKRENEEIAQKALFECERGGAPKATTDQFKCGRCGQRKTTYYQLQTRSADEP 360

Query: 361 MTTFVTCVNCNNHWKFC 378
           MTTFVTCVNCNNHWKFC
Sbjct: 361 MTTFVTCVNCNNHWKFC 374

BLAST of Lag0000137 vs. ExPASy Swiss-Prot
Match: Q9ZVH8 (Transcription elongation factor TFIIS OS=Arabidopsis thaliana OX=3702 GN=TFIIS PE=1 SV=1)

HSP 1 Score: 393.7 bits (1010), Expect = 2.4e-108
Identity = 222/384 (57.81%), Postives = 268/384 (69.79%), Query Frame = 0

Query: 1   MENELVELFEVAKKAADAAAASS-NDGGVEESRCLDALRQLKKFPVTYQILVSTQVGKRL 60
           ME++L++LFE AKKAADAAA       G E S+C+DAL+QLKKFPVTY  LV+TQVGK+L
Sbjct: 1   MESDLIDLFEGAKKAADAAALDGVTSSGPEVSQCIDALKQLKKFPVTYDTLVATQVGKKL 60

Query: 61  RHLTKHPRRKIQDYASDLIEMWKDIVIKETNKNKKNGNGNANCKEPPKIGSPSAES---- 120
           R L KHP   I+  A+DL+E+WK +VI+ET K KK   G   CKE  K+     E     
Sbjct: 61  RSLAKHPVEDIKSVATDLLEIWKKVVIEETAKAKKT-EGTNGCKE-AKVNKMDVEKPSNP 120

Query: 121 --VKVEKFQKSSSMKVEKVSKVEHFDWNGATSSVKFSRSESAISEKNSVKVEKTDSVVKV 180
             VKV+K Q+  S K  KV + E    N   + VK  R    I   N  K++     VK 
Sbjct: 121 APVKVQKLQRGDSAKSIKVERKE--PDNKVVTGVKIERKVPDIKVTNGTKIDYRGQAVKD 180

Query: 181 ERIVKEEKKISIEKKPSGGAAAPPKLTSMIKSKDAARDKIRELLFEAFSKVPGEAGEDVI 240
           E++ K+ +  S  K P+  A APPKLT+M+K  D  RDKIRELL EA  +V GEA +   
Sbjct: 181 EKVSKDNQ--SSMKAPAKAANAPPKLTAMLKCNDPVRDKIRELLVEALCRVAGEADDYER 240

Query: 241 DEVNASDPIRVAVSVESVMFENWGGSTGAQKAKYRSVMFNLKDPKNPDFRRKVLLGLIKP 300
           + VNASDP+RVAVSVES+MFE  G STGAQK KYRS+MFNL+D  NPD RR+VL G I P
Sbjct: 241 ESVNASDPLRVAVSVESLMFEKLGRSTGAQKLKYRSIMFNLRDSNNPDLRRRVLTGEISP 300

Query: 301 ERLINLSTTDMASDQRKRENEEIAQKALFDCERGGAPKATTDQFKCGRCGQRKTTYYQLQ 360
           E+LI LS  DMASD+RK+EN +I +KALFDCERG A KA+TDQFKCGRCGQRK TYYQ+Q
Sbjct: 301 EKLITLSAEDMASDKRKQENNQIKEKALFDCERGLAAKASTDQFKCGRCGQRKCTYYQMQ 360

Query: 361 TRSADEPMTTFVTCVNCNNHWKFC 378
           TRSADEPMTT+VTCVNC+NHWKFC
Sbjct: 361 TRSADEPMTTYVTCVNCDNHWKFC 378

BLAST of Lag0000137 vs. ExPASy Swiss-Prot
Match: P20232 (Transcription elongation factor S-II OS=Drosophila melanogaster OX=7227 GN=TfIIS PE=2 SV=1)

HSP 1 Score: 132.1 bits (331), Expect = 1.3e-29
Identity = 109/374 (29.14%), Postives = 160/374 (42.78%), Query Frame = 0

Query: 7   ELFEVAKKAADAAAASSNDGGVEESRCLDALRQLKKFPVTYQILVSTQVGKRLRHLTKHP 66
           E+F + KK +  A+      G  + + LD L+ L+   +   IL  T++G  +  L K  
Sbjct: 6   EVFRIQKKMSKMAS-----DGTGQDQALDLLKALQTLNINLDILTKTRIGMTVNELRKSS 65

Query: 67  R-RKIQDYASDLIEMWKDIVIKETNKNKKNGNGNANCKEPPKIGSPSAESVKVEKFQKSS 126
           +  ++   A  LI+ WK  +             N++ KE     S +++S    K   S 
Sbjct: 66  KDDEVIALAKTLIKNWKRFLASPAPTTP----NNSSAKEGSSNNSSASKSTSAAKSSSSI 125

Query: 127 SMKVEKVSKVEHFDWNGATSSVKFSRSESAISEKNSVKVEKTDSVVKVERIVKEEKKISI 186
           S K                      +S S+ S K+  K   T S              S 
Sbjct: 126 SGK---------------------DKSSSSSSSKDKEKKGSTSS--------------SQ 185

Query: 187 EKKPSGGAAAPPKLTSMIKSKDAARDKIRELLFEA--FSKVPGEAGEDVIDEVNASDPIR 246
              PSGG              DA R K RE+L  A    +VP   GE          P  
Sbjct: 186 TSFPSGGMT------------DAVRIKCREMLATALKIGEVPEGCGE----------PEE 245

Query: 247 VAVSVESVMFENWGGSTGAQKAKYRSVMFNLKDPKNPDFRRKVLLGLIKPERLINLSTTD 306
           +A  +E  ++  +  +    K + RS + NLKDPKNP  R   + G +  ++L  ++  +
Sbjct: 246 MAAELEDAIYSEFNNTDMKYKNRIRSRVANLKDPKNPGLRGNFMCGAVTAKQLAKMTPEE 305

Query: 307 MASDQRKRENEEIAQKALFDCERGGAPKATTDQFKCGRCGQRKTTYYQLQTRSADEPMTT 366
           MASD+ K+  E+  ++A+ D +        TD  KC +C +R  TY QLQTRSADEPMTT
Sbjct: 306 MASDEMKKLREKFVKEAINDAQLATVQGTKTDLLKCAKCKKRNCTYNQLQTRSADEPMTT 313

Query: 367 FVTCVNCNNHWKFC 378
           FV C  C N WKFC
Sbjct: 366 FVMCNECGNRWKFC 313

BLAST of Lag0000137 vs. ExPASy Swiss-Prot
Match: Q2KI09 (Transcription elongation factor A protein 3 OS=Bos taurus OX=9913 GN=TCEA3 PE=2 SV=1)

HSP 1 Score: 125.2 bits (313), Expect = 1.6e-27
Identity = 108/380 (28.42%), Postives = 171/380 (45.00%), Query Frame = 0

Query: 7   ELFEVAKKAADAAAASSNDGGVEESRCLDALRQLKKFPVTYQILVSTQVGKRLRHLTKH- 66
           EL  +AKK     A    +G       LD L++L  + ++ Q+L +T++G  +  + KH 
Sbjct: 6   ELLRIAKKLEKMVARKKTEG------ALDLLKKLGSWQMSIQLLQTTRIGVAVNGVRKHC 65

Query: 67  PRRKIQDYASDLIEMWKDIVIKETNKNKKNGNGNANCKEPPKIGSPSAESVKVEKFQKSS 126
             +++   A  LI  WK ++        + G      K+  K        +    ++  +
Sbjct: 66  SNKEVVALAKVLIRNWKQLLDSPATPKGEKGEERVKAKKKEK-------GLDCSDWKPET 125

Query: 127 SMKVEKVSKVEH--------FDWNGATSSVKFSRSESAISEKNSVKVEKTDSVVKVERIV 186
           S+   +  +VE            + ATSS K    E + S K+  +  +T S        
Sbjct: 126 SLSPPRKKRVEEPKDRRDSVDSKSSATSSPKRPSMERSNSSKSKAETPRTPS-------- 185

Query: 187 KEEKKISIEKKPSGGAAAPPKLTSMIKSKDAARDKIRELLFEAFSKVPGEAGEDVIDEVN 246
                 S    PS    AP  LT      D+ RDK  E+L  A      +A +D  D   
Sbjct: 186 ---SPSSPTFAPSVCLLAPCYLTG-----DSVRDKCVEMLSAAL-----KADDDYKDYGV 245

Query: 247 ASDPIRVAVSVESVMFENWGGSTGAQKAKYRSVMFNLKDPKNPDFRRKVLLGLIKPERLI 306
             D  ++A  +E  +++    +    + + RS + NLKDP+NP  RR VL G I    + 
Sbjct: 246 NCD--KMASEIEDHIYQELKSTDMKYRNRVRSRISNLKDPRNPGLRRNVLSGAISAGLIA 305

Query: 307 NLSTTDMASDQRKRENEEIAQKALFDCERGGAPKATTDQFKCGRCGQRKTTYYQLQTRSA 366
            ++  +MASD+ +     + Q+A+ + +       TTD F+C +C ++  TY Q+QTRSA
Sbjct: 306 KMTAEEMASDELRELRNAMTQEAIREHQMAKTGGTTTDLFQCSKCKKKNCTYNQVQTRSA 349

Query: 367 DEPMTTFVTCVNCNNHWKFC 378
           DEPMTTFV C  C N WKFC
Sbjct: 366 DEPMTTFVLCNECGNRWKFC 349

BLAST of Lag0000137 vs. ExPASy Swiss-Prot
Match: O75764 (Transcription elongation factor A protein 3 OS=Homo sapiens OX=9606 GN=TCEA3 PE=1 SV=2)

HSP 1 Score: 122.5 bits (306), Expect = 1.0e-26
Identity = 111/382 (29.06%), Postives = 175/382 (45.81%), Query Frame = 0

Query: 7   ELFEVAKKAADAAAASSNDGGVEESRCLDALRQLKKFPVTYQILVSTQVGKRLRHLTKH- 66
           EL  +AKK     A  + +G       LD L++L    ++ Q+L +T++G  +  + KH 
Sbjct: 6   ELLRIAKKLEKMVARKNTEG------ALDLLKKLHSCQMSIQLLQTTRIGVAVNGVRKHC 65

Query: 67  PRRKIQDYASDLIEMWKDIVIKETNKNKKNGNGNANCKEPPKIGSPSAESVKVEKFQKSS 126
             +++   A  LI+ WK ++               +   PPK G    E  K +K +K  
Sbjct: 66  SDKEVVSLAKVLIKNWKRLL---------------DSPGPPK-GEKGEEREKAKKKEKG- 125

Query: 127 SMKVEKVSKVEHFDWN-GATSSVKFSRSESAISEKNSVKVEKTDSV----VKVERIVKEE 186
                    +E  DW   A  S    + E   + ++SV  + + S       VER    +
Sbjct: 126 ---------LECSDWKPEAGLSPPRKKREDPKTRRDSVDSKSSASSSPKRPSVERSNSSK 185

Query: 187 KKISIEKKPSGG-----AAAPPKLTSMIKSKDAARDKIRELLFEAFSKVPGEAGEDVIDE 246
            K    K PS       A++   L     + D+ RDK  E+L  A      +A +D  D 
Sbjct: 186 SKAESPKTPSSPLTPTFASSMCLLAPCYLTGDSVRDKCVEMLSAAL-----KADDDYKDY 245

Query: 247 VNASDPIRVAVSVESVMFENWGGSTGAQKAKYRSVMFNLKDPKNPDFRRKVLLGLIKPER 306
               D  ++A  +E  +++    +    + + RS + NLKDP+NP  RR VL G I    
Sbjct: 246 GVNCD--KMASEIEDHIYQELKSTDMKYRNRVRSRISNLKDPRNPGLRRNVLSGAISAGL 305

Query: 307 LINLSTTDMASDQRKRENEEIAQKALFDCERGGAPKATTDQFKCGRCGQRKTTYYQLQTR 366
           +  ++  +MASD+ +     + Q+A+ + +       TTD F+C +C ++  TY Q+QTR
Sbjct: 306 IAKMTAEEMASDELRELRNAMTQEAIREHQMAKTGGTTTDLFQCSKCKKKNCTYNQVQTR 348

Query: 367 SADEPMTTFVTCVNCNNHWKFC 378
           SADEPMTTFV C  C N WKFC
Sbjct: 366 SADEPMTTFVLCNECGNRWKFC 348

BLAST of Lag0000137 vs. ExPASy Swiss-Prot
Match: P23881 (Transcription elongation factor A protein 3 OS=Mus musculus OX=10090 GN=Tcea3 PE=1 SV=3)

HSP 1 Score: 122.1 bits (305), Expect = 1.4e-26
Identity = 110/382 (28.80%), Postives = 172/382 (45.03%), Query Frame = 0

Query: 7   ELFEVAKKAADAAAASSNDGGVEESRCLDALRQLKKFPVTYQILVSTQVGKRLRHLTKH- 66
           EL  +AKK     +    +G       LD L++L    ++ Q+L +T++G  +  + KH 
Sbjct: 6   ELLRIAKKLEKMVSRKKTEG------ALDLLKKLNSCQMSIQLLQTTRIGVAVNGVRKHC 65

Query: 67  PRRKIQDYASDLIEMWKDIV---------IKETNKNKK-NGNGNANCKEPPKIGSPSAES 126
             +++   A  LI+ WK ++          +E  K KK  G G ++ K    +  P  + 
Sbjct: 66  SDKEVVSLAKVLIKNWKRLLDSPRTTKGEREEREKAKKEKGLGCSDWKPEAGLSPPRKKG 125

Query: 127 VKVEKFQKSSSMKVEKVSKVEHFDWNGATSSVKFSRSESAISEKNSVKVEKTDSVVKVER 186
               K ++ S   V+  S          TSS K    E + S K+ V+   T S      
Sbjct: 126 GGEPKTRRDS---VDSRS--------STTSSPKRPSLERSNSSKSKVETPTTPSSPSTPT 185

Query: 187 IVKEEKKISIEKKPSGGAAAPPKLTSMIKSKDAARDKIRELLFEAFSKVPGEAGEDVIDE 246
                        P+    AP  LT      D+ RDK  E+L  A         ED   +
Sbjct: 186 FA-----------PAVCLLAPCYLTG-----DSVRDKCVEMLSAALK------AEDNFKD 245

Query: 247 VNASDPIRVAVSVESVMFENWGGSTGAQKAKYRSVMFNLKDPKNPDFRRKVLLGLIKPER 306
              +   ++A  +E  +++    +    + + RS + NLKDP+NP  RR VL G I PE 
Sbjct: 246 YGVNCD-KLASEIEDHIYQELKSTDMKYRNRVRSRISNLKDPRNPGLRRNVLSGAISPEL 305

Query: 307 LINLSTTDMASDQRKRENEEIAQKALFDCERGGAPKATTDQFKCGRCGQRKTTYYQLQTR 366
           +  ++  +MASD+ +     + Q+A+ + +       TTD  +C +C ++  TY Q+QTR
Sbjct: 306 IAKMTAEEMASDELRELRNAMTQEAIREHQMAKTGGTTTDLLRCSKCKKKNCTYNQVQTR 347

Query: 367 SADEPMTTFVTCVNCNNHWKFC 378
           SADEPMTTFV C  C N WKFC
Sbjct: 366 SADEPMTTFVLCNECGNRWKFC 347

BLAST of Lag0000137 vs. ExPASy TrEMBL
Match: A0A6J1GYA3 (transcription elongation factor TFIIS-like OS=Cucurbita moschata OX=3662 GN=LOC111458597 PE=4 SV=1)

HSP 1 Score: 668.7 bits (1724), Expect = 1.4e-188
Identity = 350/377 (92.84%), Postives = 360/377 (95.49%), Query Frame = 0

Query: 1   MENELVELFEVAKKAADAAAASSNDGGVEESRCLDALRQLKKFPVTYQILVSTQVGKRLR 60
           ME ELVELFE AKKAADAAAA SNDGG EESRCLDALRQLKKFPVTYQILVSTQVGKRLR
Sbjct: 1   MEKELVELFEAAKKAADAAAARSNDGGAEESRCLDALRQLKKFPVTYQILVSTQVGKRLR 60

Query: 61  HLTKHPRRKIQDYASDLIEMWKDIVIKETNKNKKNGNGNANCKEPPKIGSPSAESVKVEK 120
           HLTKHP++KIQDYASDLIEMWKDIVIKETNKNKKNGNGN+  KEP KIGSPSAESVKVE 
Sbjct: 61  HLTKHPKKKIQDYASDLIEMWKDIVIKETNKNKKNGNGNS--KEPSKIGSPSAESVKVEN 120

Query: 121 FQKSSSMKVEKVSKVEHFDWNGATSSVKFSRSESAISEKNSVKVEKTDSVVKVERIVKEE 180
            QKSSSMKVE+VSKVE FD NG+TSSVKFSRS SA+SEKNSVKVEKTDSVVKVER+VKEE
Sbjct: 121 LQKSSSMKVERVSKVEQFDRNGSTSSVKFSRSASAVSEKNSVKVEKTDSVVKVERMVKEE 180

Query: 181 KKISIEKKPSGGAAAPPKLTSMIKSKDAARDKIRELLFEAFSKVPGEAGEDVIDEVNASD 240
           KKISIEKKPS GAA PPKLTSMIKSKDAARDKIRELL EAFSKVPGEA EDVIDEVNASD
Sbjct: 181 KKISIEKKPS-GAAGPPKLTSMIKSKDAARDKIRELLVEAFSKVPGEADEDVIDEVNASD 240

Query: 241 PIRVAVSVESVMFENWGGSTGAQKAKYRSVMFNLKDPKNPDFRRKVLLGLIKPERLINLS 300
           PIRVAVSVESVMFENWGGSTGAQKAKYRS+MFNLKDPKNPDFRRKVLLGLIKPER+ NLS
Sbjct: 241 PIRVAVSVESVMFENWGGSTGAQKAKYRSIMFNLKDPKNPDFRRKVLLGLIKPERMTNLS 300

Query: 301 TTDMASDQRKRENEEIAQKALFDCERGGAPKATTDQFKCGRCGQRKTTYYQLQTRSADEP 360
           TTDMASDQRKRENEEIAQKALF+CERGGAPKATTDQFKCGRCGQRKTTYYQLQTRSADEP
Sbjct: 301 TTDMASDQRKRENEEIAQKALFECERGGAPKATTDQFKCGRCGQRKTTYYQLQTRSADEP 360

Query: 361 MTTFVTCVNCNNHWKFC 378
           MTTFVTCVNCNNHWKFC
Sbjct: 361 MTTFVTCVNCNNHWKFC 374

BLAST of Lag0000137 vs. ExPASy TrEMBL
Match: A0A6J1JLK7 (transcription elongation factor TFIIS-like OS=Cucurbita maxima OX=3661 GN=LOC111486982 PE=4 SV=1)

HSP 1 Score: 665.6 bits (1716), Expect = 1.2e-187
Identity = 349/377 (92.57%), Postives = 360/377 (95.49%), Query Frame = 0

Query: 1   MENELVELFEVAKKAADAAAASSNDGGVEESRCLDALRQLKKFPVTYQILVSTQVGKRLR 60
           ME ELVELFE AKKAADAAAA SNDGG EESRCLDALRQLKKFPVTYQILVSTQVGKRLR
Sbjct: 1   MEKELVELFEAAKKAADAAAAPSNDGGAEESRCLDALRQLKKFPVTYQILVSTQVGKRLR 60

Query: 61  HLTKHPRRKIQDYASDLIEMWKDIVIKETNKNKKNGNGNANCKEPPKIGSPSAESVKVEK 120
           HLTKHP++KIQDYASDLIEMWKDIVIKETNKNKKNGNGN+  KEP KIGSPSAESVKVE 
Sbjct: 61  HLTKHPKKKIQDYASDLIEMWKDIVIKETNKNKKNGNGNS--KEPSKIGSPSAESVKVEN 120

Query: 121 FQKSSSMKVEKVSKVEHFDWNGATSSVKFSRSESAISEKNSVKVEKTDSVVKVERIVKEE 180
           FQKSSSMKVE+VSKVE F+  G+TSSVKFSRS SA+SEKNSVKVEKTDSVVKVER+VKEE
Sbjct: 121 FQKSSSMKVERVSKVEQFNRIGSTSSVKFSRSASAVSEKNSVKVEKTDSVVKVERMVKEE 180

Query: 181 KKISIEKKPSGGAAAPPKLTSMIKSKDAARDKIRELLFEAFSKVPGEAGEDVIDEVNASD 240
           KKISIEKKPS GAA PPKLTSMIKSKDAARDKIRELL EAFSKVPGEA EDVIDEVNASD
Sbjct: 181 KKISIEKKPS-GAAGPPKLTSMIKSKDAARDKIRELLVEAFSKVPGEADEDVIDEVNASD 240

Query: 241 PIRVAVSVESVMFENWGGSTGAQKAKYRSVMFNLKDPKNPDFRRKVLLGLIKPERLINLS 300
           PIRVAVSVESVMFENWGGSTGAQKAKYRS+MFNLKDPKNPDFRRKVLLGLIKPER+ NLS
Sbjct: 241 PIRVAVSVESVMFENWGGSTGAQKAKYRSIMFNLKDPKNPDFRRKVLLGLIKPERMTNLS 300

Query: 301 TTDMASDQRKRENEEIAQKALFDCERGGAPKATTDQFKCGRCGQRKTTYYQLQTRSADEP 360
           TTDMASDQRKRENEEIAQKALF+CERGGAPKATTDQFKCGRCGQRKTTYYQLQTRSADEP
Sbjct: 301 TTDMASDQRKRENEEIAQKALFECERGGAPKATTDQFKCGRCGQRKTTYYQLQTRSADEP 360

Query: 361 MTTFVTCVNCNNHWKFC 378
           MTTFVTCVNCNNHWKFC
Sbjct: 361 MTTFVTCVNCNNHWKFC 374

BLAST of Lag0000137 vs. ExPASy TrEMBL
Match: A0A6J1BWY6 (transcription elongation factor TFIIS OS=Momordica charantia OX=3673 GN=LOC111006416 PE=4 SV=1)

HSP 1 Score: 657.9 bits (1696), Expect = 2.5e-185
Identity = 342/379 (90.24%), Postives = 356/379 (93.93%), Query Frame = 0

Query: 1   MENELVELFEVAKKAADAAAASSNDGGVEESRCLDALRQLKKFPVTYQILVSTQVGKRLR 60
           MENELVELFE AKKAADAAAA SNDGG EESRCLDALRQLKKFPVTYQILVSTQVGKRLR
Sbjct: 1   MENELVELFEAAKKAADAAAAPSNDGGAEESRCLDALRQLKKFPVTYQILVSTQVGKRLR 60

Query: 61  HLTKHPRRKIQDYASDLIEMWKDIVIKETNKNKKNGNGNANCKEPPKIGSPSAESVKVEK 120
           HLTKHP++KIQDYASDLIEMWK+IVIKETNKNKKNGNG  NCKE PKIGSPSAESVKVEK
Sbjct: 61  HLTKHPKKKIQDYASDLIEMWKEIVIKETNKNKKNGNG--NCKESPKIGSPSAESVKVEK 120

Query: 121 FQKSSSMKVEKVSKVEHFDWNGATSSVKFSRSESAISEKNSVKVEKTDSV--VKVERIVK 180
           FQKSSSMKVE+VSKVE  D NG TSSVK+SRSES ISEKN VKVEKTDS+  VK ER+VK
Sbjct: 121 FQKSSSMKVERVSKVEQSDRNGTTSSVKYSRSESVISEKNIVKVEKTDSLEKVKAERVVK 180

Query: 181 EEKKISIEKKPSGGAAAPPKLTSMIKSKDAARDKIRELLFEAFSKVPGEAGEDVIDEVNA 240
           EEKKISI+K+PS G AAPPKLTSMIKSKDAARDKIRELL EAFSKVPGEA EDVIDEVNA
Sbjct: 181 EEKKISIDKRPSSGTAAPPKLTSMIKSKDAARDKIRELLCEAFSKVPGEADEDVIDEVNA 240

Query: 241 SDPIRVAVSVESVMFENWGGSTGAQKAKYRSVMFNLKDPKNPDFRRKVLLGLIKPERLIN 300
           SDP+RVAVSVESVMFENWGGSTGAQKAKYRS+MFNLKDPKNPDFRR VL+G IKPERLIN
Sbjct: 241 SDPVRVAVSVESVMFENWGGSTGAQKAKYRSIMFNLKDPKNPDFRRNVLVGHIKPERLIN 300

Query: 301 LSTTDMASDQRKRENEEIAQKALFDCERGGAPKATTDQFKCGRCGQRKTTYYQLQTRSAD 360
           +ST +MASDQRKRENEEI QKALFDCERGGAPKATTDQFKCGRCGQRKTTYYQLQTRSAD
Sbjct: 301 MSTAEMASDQRKRENEEIEQKALFDCERGGAPKATTDQFKCGRCGQRKTTYYQLQTRSAD 360

Query: 361 EPMTTFVTCVNCNNHWKFC 378
           EPMTTFVTCVNCNNHWKFC
Sbjct: 361 EPMTTFVTCVNCNNHWKFC 377

BLAST of Lag0000137 vs. ExPASy TrEMBL
Match: A0A0A0KDH9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G014610 PE=4 SV=1)

HSP 1 Score: 652.9 bits (1683), Expect = 8.1e-184
Identity = 338/377 (89.66%), Postives = 357/377 (94.69%), Query Frame = 0

Query: 1   MENELVELFEVAKKAADAAAASSNDGGVEESRCLDALRQLKKFPVTYQILVSTQVGKRLR 60
           MENELVELFE AKKAADAAAA SNDGG EESRCLDALRQLKKFPVTYQILVSTQVGKRLR
Sbjct: 1   MENELVELFEAAKKAADAAAAPSNDGGAEESRCLDALRQLKKFPVTYQILVSTQVGKRLR 60

Query: 61  HLTKHPRRKIQDYASDLIEMWKDIVIKETNKNKKNGNGNANCKEPPKIGSPSAESVKVEK 120
           HLTKHP++KIQ++ASDLIEMWK+IVIKETNKNKK  NGNA+ K+ PKIGSPSAESVKVEK
Sbjct: 61  HLTKHPKKKIQEHASDLIEMWKEIVIKETNKNKK--NGNASSKDSPKIGSPSAESVKVEK 120

Query: 121 FQKSSSMKVEKVSKVEHFDWNGATSSVKFSRSESAISEKNSVKVEKTDSVVKVERIVKEE 180
           FQKSSSMKVE+VSKVE FD NGATSSVK+S+SES +SE+NSVKVEKTDS+VKVER+VKE 
Sbjct: 121 FQKSSSMKVERVSKVEQFDRNGATSSVKYSKSESVVSERNSVKVEKTDSMVKVERVVKE- 180

Query: 181 KKISIEKKPSGGAAAPPKLTSMIKSKDAARDKIRELLFEAFSKVPGEAGEDVIDEVNASD 240
                EKKPS GAAAPPKLTSMIKSKDAARDKIRELLFEAFSKVPGEA E+ +DEVNASD
Sbjct: 181 -----EKKPSSGAAAPPKLTSMIKSKDAARDKIRELLFEAFSKVPGEADEEFMDEVNASD 240

Query: 241 PIRVAVSVESVMFENWGGSTGAQKAKYRSVMFNLKDPKNPDFRRKVLLGLIKPERLINLS 300
           PIRVAVSVESVMFENWGGSTGAQKAKYRS+MFNLKDPKNPDFRRKVLLGLIKPER+IN+S
Sbjct: 241 PIRVAVSVESVMFENWGGSTGAQKAKYRSIMFNLKDPKNPDFRRKVLLGLIKPERMINMS 300

Query: 301 TTDMASDQRKRENEEIAQKALFDCERGGAPKATTDQFKCGRCGQRKTTYYQLQTRSADEP 360
           T DMASDQRKRENEEIAQKALFDCERGGAPKATTDQFKCGRCGQRKTTYYQLQTRSADEP
Sbjct: 301 TADMASDQRKRENEEIAQKALFDCERGGAPKATTDQFKCGRCGQRKTTYYQLQTRSADEP 360

Query: 361 MTTFVTCVNCNNHWKFC 378
           MTTFVTCVNCNNHWKFC
Sbjct: 361 MTTFVTCVNCNNHWKFC 369

BLAST of Lag0000137 vs. ExPASy TrEMBL
Match: A0A6J1FF11 (transcription elongation factor TFIIS-like OS=Cucurbita moschata OX=3662 GN=LOC111444888 PE=4 SV=1)

HSP 1 Score: 652.9 bits (1683), Expect = 8.1e-184
Identity = 338/377 (89.66%), Postives = 354/377 (93.90%), Query Frame = 0

Query: 1   MENELVELFEVAKKAADAAAASSNDGGVEESRCLDALRQLKKFPVTYQILVSTQVGKRLR 60
           MENELVELFE AKKAADAAAA SNDGG EESRCLDALRQLKKFPVTYQILVSTQVGKRLR
Sbjct: 1   MENELVELFEAAKKAADAAAAPSNDGGAEESRCLDALRQLKKFPVTYQILVSTQVGKRLR 60

Query: 61  HLTKHPRRKIQDYASDLIEMWKDIVIKETNKNKKNGNGNANCKEPPKIGSPSAESVKVEK 120
           HLTKHP++KIQ+YASDLIE+WK+IVIK+TNKNKK  NGN +C+E PKIGSPSAESVKVEK
Sbjct: 61  HLTKHPKKKIQNYASDLIEIWKEIVIKDTNKNKK--NGNIDCEESPKIGSPSAESVKVEK 120

Query: 121 FQKSSSMKVEKVSKVEHFDWNGATSSVKFSRSESAISEKNSVKVEKTDSVVKVERIVKEE 180
           F+KSSSMKVE+VS+VE FD NG TSS KFSRSES IS KN VKVEKTDSVVKVERIVKEE
Sbjct: 121 FRKSSSMKVERVSQVEQFDRNGVTSSAKFSRSESDISGKNPVKVEKTDSVVKVERIVKEE 180

Query: 181 KKISIEKKPSGGAAAPPKLTSMIKSKDAARDKIRELLFEAFSKVPGEAGEDVIDEVNASD 240
           K+ISIEKKPS G  APPKLTSMIKSKDAARDKIRELLFEAFSKVP EA EDV+DEVNASD
Sbjct: 181 KRISIEKKPSSGPDAPPKLTSMIKSKDAARDKIRELLFEAFSKVPSEADEDVMDEVNASD 240

Query: 241 PIRVAVSVESVMFENWGGSTGAQKAKYRSVMFNLKDPKNPDFRRKVLLGLIKPERLINLS 300
           PIRVAVSVESVMFENWGGSTG+QK KYRS+MFNLKDPKNPDFRRKVLLGLIKPERLIN+S
Sbjct: 241 PIRVAVSVESVMFENWGGSTGSQKTKYRSIMFNLKDPKNPDFRRKVLLGLIKPERLINMS 300

Query: 301 TTDMASDQRKRENEEIAQKALFDCERGGAPKATTDQFKCGRCGQRKTTYYQLQTRSADEP 360
           T DMASDQRKRENEEI QKALFDCER GAPKATTDQFKCGRCGQRKTTYYQLQTRSADEP
Sbjct: 301 TADMASDQRKRENEEIEQKALFDCERAGAPKATTDQFKCGRCGQRKTTYYQLQTRSADEP 360

Query: 361 MTTFVTCVNCNNHWKFC 378
           MTTFVTCVNCNNHWKFC
Sbjct: 361 MTTFVTCVNCNNHWKFC 375

BLAST of Lag0000137 vs. TAIR 10
Match: AT2G38560.1 (transcript elongation factor IIS )

HSP 1 Score: 393.7 bits (1010), Expect = 1.7e-109
Identity = 222/384 (57.81%), Postives = 268/384 (69.79%), Query Frame = 0

Query: 1   MENELVELFEVAKKAADAAAASS-NDGGVEESRCLDALRQLKKFPVTYQILVSTQVGKRL 60
           ME++L++LFE AKKAADAAA       G E S+C+DAL+QLKKFPVTY  LV+TQVGK+L
Sbjct: 1   MESDLIDLFEGAKKAADAAALDGVTSSGPEVSQCIDALKQLKKFPVTYDTLVATQVGKKL 60

Query: 61  RHLTKHPRRKIQDYASDLIEMWKDIVIKETNKNKKNGNGNANCKEPPKIGSPSAES---- 120
           R L KHP   I+  A+DL+E+WK +VI+ET K KK   G   CKE  K+     E     
Sbjct: 61  RSLAKHPVEDIKSVATDLLEIWKKVVIEETAKAKKT-EGTNGCKE-AKVNKMDVEKPSNP 120

Query: 121 --VKVEKFQKSSSMKVEKVSKVEHFDWNGATSSVKFSRSESAISEKNSVKVEKTDSVVKV 180
             VKV+K Q+  S K  KV + E    N   + VK  R    I   N  K++     VK 
Sbjct: 121 APVKVQKLQRGDSAKSIKVERKE--PDNKVVTGVKIERKVPDIKVTNGTKIDYRGQAVKD 180

Query: 181 ERIVKEEKKISIEKKPSGGAAAPPKLTSMIKSKDAARDKIRELLFEAFSKVPGEAGEDVI 240
           E++ K+ +  S  K P+  A APPKLT+M+K  D  RDKIRELL EA  +V GEA +   
Sbjct: 181 EKVSKDNQ--SSMKAPAKAANAPPKLTAMLKCNDPVRDKIRELLVEALCRVAGEADDYER 240

Query: 241 DEVNASDPIRVAVSVESVMFENWGGSTGAQKAKYRSVMFNLKDPKNPDFRRKVLLGLIKP 300
           + VNASDP+RVAVSVES+MFE  G STGAQK KYRS+MFNL+D  NPD RR+VL G I P
Sbjct: 241 ESVNASDPLRVAVSVESLMFEKLGRSTGAQKLKYRSIMFNLRDSNNPDLRRRVLTGEISP 300

Query: 301 ERLINLSTTDMASDQRKRENEEIAQKALFDCERGGAPKATTDQFKCGRCGQRKTTYYQLQ 360
           E+LI LS  DMASD+RK+EN +I +KALFDCERG A KA+TDQFKCGRCGQRK TYYQ+Q
Sbjct: 301 EKLITLSAEDMASDKRKQENNQIKEKALFDCERGLAAKASTDQFKCGRCGQRKCTYYQMQ 360

Query: 361 TRSADEPMTTFVTCVNCNNHWKFC 378
           TRSADEPMTT+VTCVNC+NHWKFC
Sbjct: 361 TRSADEPMTTYVTCVNCDNHWKFC 378

BLAST of Lag0000137 vs. TAIR 10
Match: AT2G42730.1 (F-box family protein )

HSP 1 Score: 100.9 bits (250), Expect = 2.3e-21
Identity = 57/127 (44.88%), Postives = 79/127 (62.20%), Query Frame = 0

Query: 197 PKLTSMIKSKDAARDKIRELLFEAFSKVPGE-AGEDVIDEVNASDPIRVAVSVESVMFEN 256
           P  ++M K+ D+ RDK+RE+L  +  KV  E    ++   V A DP  VAVSVES MFE 
Sbjct: 594 PTHSTMKKTGDSKRDKVREILQTSLVKVASEIVDTEMKTRVTACDPSVVAVSVESAMFEK 653

Query: 257 WGGSTGAQKAKYRSVMFNLKDPKNPDFRRKVLLGLIKPERLINLSTTDMASDQRKRENEE 316
            G   G  KAKYRS++FN+ D  NPD RRKVL+G I  ERL+ +   +M S++ ++E + 
Sbjct: 654 LGCFMGPHKAKYRSILFNMGDSNNPDLRRKVLIGEINGERLVTMERQEMGSEKIQKEVQR 713

Query: 317 IAQKALF 323
           I + A F
Sbjct: 714 IKENARF 720

BLAST of Lag0000137 vs. TAIR 10
Match: AT4G18720.1 (Transcription factor IIS protein )

HSP 1 Score: 98.6 bits (244), Expect = 1.1e-20
Identity = 55/111 (49.55%), Postives = 72/111 (64.86%), Query Frame = 0

Query: 197 PKLTSMIKSKDAARDKIRELLFEAFSKVPGEAGE-DVIDEVNASDPIRVAVSVESVMFEN 256
           P   +M K+ D+ RDK+RE+L  + +KV  E  + ++   V A DP  VAVSVE+ MFEN
Sbjct: 105 PTHATMKKTGDSKRDKVREILQTSLAKVASEVVDTEMKTRVTACDPWVVAVSVETAMFEN 164

Query: 257 WGGSTGAQKAKYRSVMFNLKDPKNPDFRRKVLLGLIKPERLINLSTTDMAS 307
            G   G QKAKYRS++FN+ D  NPD RRKVLLG I  ERL+ +   +M S
Sbjct: 165 LGCFMGPQKAKYRSILFNMGDSNNPDLRRKVLLGEISGERLVKMEKEEMGS 215

BLAST of Lag0000137 vs. TAIR 10
Match: AT5G42325.1 (Transcription factor IIS protein )

HSP 1 Score: 70.9 bits (172), Expect = 2.5e-12
Identity = 45/128 (35.16%), Postives = 70/128 (54.69%), Query Frame = 0

Query: 197 PKLTSMIKSKDAARDKIRELLFEAFSKVPGEAGEDVIDE--VNASDPIRVAVSVESVMFE 256
           P  ++M K+ D+ RDK+ E+L  + +KV  E  +  +    +   DP  VAVSVES M  
Sbjct: 104 PTHSTMKKTGDSKRDKVHEILQSSLAKVATEVVDTEMKRRVMTVCDPWVVAVSVESAM-- 163

Query: 257 NWGGSTGAQKAKYRSVMFNLKDPKNPDFRRKVLLGLIKPERLINLSTTDMASDQRKRENE 316
                         S++FN+ D  NPD RRKVL+G I  ERL+ +   +M S++ ++E +
Sbjct: 164 --------------SILFNMGDSNNPDLRRKVLIGEISGERLVKMEKDEMGSEKIQKEVQ 215

Query: 317 EIAQKALF 323
            I ++A F
Sbjct: 224 RIKERARF 215

BLAST of Lag0000137 vs. TAIR 10
Match: AT2G33090.1 (Transcription elongation factor (TFIIS) family protein )

HSP 1 Score: 63.9 bits (154), Expect = 3.1e-10
Identity = 35/98 (35.71%), Postives = 59/98 (60.20%), Query Frame = 0

Query: 2   ENELVELFEVAKKAADAAAASS-NDGGVEESRCLDALRQLKKFPVTYQILVSTQVGKRLR 61
           + + +ELFE+AKK+AD A A       VE S C+D L  L +FP++ + + + ++  RL 
Sbjct: 151 DTDTLELFEIAKKSADFANAKGILASEVETSMCVDTLTLLLEFPISARDMETRKLMVRLE 210

Query: 62  HLTKHPRRKIQDYASDLIEMWKDIVIKETNKNKKNGNG 99
           HLTKH  RKI + AS L++ W++ + ++  +  +   G
Sbjct: 211 HLTKHKNRKICNSASKLLQCWRESIREQQLRESRKTQG 248

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038884917.17.8e-18990.98transcription elongation factor TFIIS [Benincasa hispida][more]
XP_022957122.13.0e-18892.84transcription elongation factor TFIIS-like [Cucurbita moschata][more]
KAG6602582.16.6e-18892.57Transcription elongation factor TFIIS, partial [Cucurbita argyrosperma subsp. so... [more]
XP_022989946.12.5e-18792.57transcription elongation factor TFIIS-like [Cucurbita maxima][more]
KAG7033260.13.3e-18792.31Transcription elongation factor TFIIS, partial [Cucurbita argyrosperma subsp. ar... [more]
Match NameE-valueIdentityDescription
Q9ZVH82.4e-10857.81Transcription elongation factor TFIIS OS=Arabidopsis thaliana OX=3702 GN=TFIIS P... [more]
P202321.3e-2929.14Transcription elongation factor S-II OS=Drosophila melanogaster OX=7227 GN=TfIIS... [more]
Q2KI091.6e-2728.42Transcription elongation factor A protein 3 OS=Bos taurus OX=9913 GN=TCEA3 PE=2 ... [more]
O757641.0e-2629.06Transcription elongation factor A protein 3 OS=Homo sapiens OX=9606 GN=TCEA3 PE=... [more]
P238811.4e-2628.80Transcription elongation factor A protein 3 OS=Mus musculus OX=10090 GN=Tcea3 PE... [more]
Match NameE-valueIdentityDescription
A0A6J1GYA31.4e-18892.84transcription elongation factor TFIIS-like OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
A0A6J1JLK71.2e-18792.57transcription elongation factor TFIIS-like OS=Cucurbita maxima OX=3661 GN=LOC111... [more]
A0A6J1BWY62.5e-18590.24transcription elongation factor TFIIS OS=Momordica charantia OX=3673 GN=LOC11100... [more]
A0A0A0KDH98.1e-18489.66Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G014610 PE=4 SV=1[more]
A0A6J1FF118.1e-18489.66transcription elongation factor TFIIS-like OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
Match NameE-valueIdentityDescription
AT2G38560.11.7e-10957.81transcript elongation factor IIS [more]
AT2G42730.12.3e-2144.88F-box family protein [more]
AT4G18720.11.1e-2049.55Transcription factor IIS protein [more]
AT5G42325.12.5e-1235.16Transcription factor IIS protein [more]
AT2G33090.13.1e-1035.71Transcription elongation factor (TFIIS) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001222Zinc finger, TFIIS-typeSMARTSM00440Cys4_2coord: 337..376
e-value: 9.7E-24
score: 94.9
IPR001222Zinc finger, TFIIS-typePFAMPF01096TFIIS_Ccoord: 337..375
e-value: 1.1E-19
score: 70.0
IPR001222Zinc finger, TFIIS-typePROSITEPS00466ZF_TFIIS_1coord: 339..374
IPR001222Zinc finger, TFIIS-typePROSITEPS51133ZF_TFIIS_2coord: 335..375
score: 14.636859
IPR003617Transcription elongation factor, TFIIS/CRSP70, N-terminal, sub-typeSMARTSM00509TFS2_5coord: 8..87
e-value: 1.6E-23
score: 94.1
IPR003618Transcription elongation factor S-II, central domainSMARTSM00510mid_6coord: 207..315
e-value: 5.5E-35
score: 132.2
IPR003618Transcription elongation factor S-II, central domainPFAMPF07500TFIIS_Mcoord: 207..323
e-value: 8.3E-31
score: 106.8
IPR003618Transcription elongation factor S-II, central domainPROSITEPS51321TFIIS_CENTRALcoord: 209..332
score: 34.197464
IPR035441TFIIS/LEDGF domain superfamilyGENE3D1.20.930.10Conserved domain common to transcription factors TFIIS, elongin A, CRSP70coord: 1..101
e-value: 3.4E-23
score: 83.2
IPR035441TFIIS/LEDGF domain superfamilySUPERFAMILY47676Conserved domain common to transcription factors TFIIS, elongin A, CRSP70coord: 29..98
NoneNo IPR availableGENE3D2.20.25.10coord: 306..377
e-value: 1.4E-27
score: 97.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 91..114
NoneNo IPR availablePANTHERPTHR11477TRANSCRIPTION FACTOR S-II ZINC FINGER DOMAIN-CONTAINING PROTEINcoord: 1..377
NoneNo IPR availablePANTHERPTHR11477:SF36TRANSCRIPTION ELONGATION FACTOR TFIIScoord: 1..377
NoneNo IPR availableCDDcd00183TFIIS_Icoord: 22..85
e-value: 2.19749E-16
score: 71.1854
NoneNo IPR availableCDDcd13749Zn-ribbon_TFIIScoord: 330..376
e-value: 3.54635E-30
score: 108.077
NoneNo IPR availableSUPERFAMILY57783Zinc beta-ribboncoord: 318..375
IPR006289Transcription elongation factor, TFIISTIGRFAMTIGR01385TIGR01385coord: 13..377
e-value: 8.8E-98
score: 325.6
IPR017923Transcription factor IIS, N-terminalPFAMPF08711Med26coord: 36..85
e-value: 5.8E-11
score: 42.2
IPR017923Transcription factor IIS, N-terminalPROSITEPS51319TFIIS_Ncoord: 10..88
score: 20.486874
IPR036575Transcription elongation factor S-II, central domain superfamilyGENE3D1.10.472.30coord: 192..305
e-value: 2.3E-25
score: 91.0
IPR036575Transcription elongation factor S-II, central domain superfamilySUPERFAMILY46942Elongation factor TFIIS domain 2coord: 196..307
IPR035100Transcription elongation factor, IIS-typePIRSFPIRSF006704TFIIScoord: 2..377
e-value: 1.7E-97
score: 324.7

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0000137.1Lag0000137.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006355 regulation of transcription, DNA-templated
biological_process GO:0006414 translational elongation
biological_process GO:0006351 transcription, DNA-templated
cellular_component GO:0005634 nucleus
molecular_function GO:0003746 translation elongation factor activity
molecular_function GO:0008270 zinc ion binding
molecular_function GO:0003676 nucleic acid binding