IVF0025894 (gene) Melon (IVF77) v1

Overview
NameIVF0025894
Typegene
OrganismCucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
DescriptionPalmitoyl-monogalactosyldiacylglycerol delta-7 desaturase
Locationchr02: 16740534 .. 16743999 (+)
RNA-Seq ExpressionIVF0025894
SyntenyIVF0025894
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
CCTAATTGACAATGGATGTATCAATGGAACCAAGTGAAATAGAAAGATCTCCAACATCATTCGGGAAGGAAGAATGGGCCGAGATACACAAAGGAATGGCTTTTATAATTTTCCTCTTGCATCTCCTTTCCATTTTTGCACCCTTTCAATTTAGTTGGAGTGCATTATGGGTTGCTGTGGCATTACATGTTATCACGGGTCTCTTTGGTATGAATCTTTCATATCACAGAAATCTTTCGCACAAGAGTTTTAAGCTTCCAAATTGGCTCGAGTACTTGTTTGCTTACTGTGGAGTTCAAGCACTTGAGGTTAATTAAACAACAAACTCTAAATTAAAACACGTTAAAGTATCTAGTATTTTTAAAGTTATATTTTATATGTACATATTTTTTAGGGAGATCCAATTGATTGGGTGAGTACGCATAGATATTACCACCAAGTTGCTGATACTAATAAAGATCCACAACGTCTTGAGCAAAGATTTTTGTTGCTTTCAAATTACTATGGATCAAATAAAAGAGTTAATTCGAGGTACTTTGCTATTTTTAAAGAGATAGAAAAGAACGTGTTTTTAATGGTTAGGAAGAAAGTGAGACGAGATAATGTTGAGGACTTGGAGAAGCAAAGTTTCTACAAATTCATTCACAAAACGTATATTATTCATCCAATTGCTCTTGCAATCTTACTATATGCAGTTGGAGGAATGCCTTTTCTTATATGGGGAATGGTAAAGTAAAACAACTCTCTCTTTGTTTGTTTCTTTTAGATTCTTAAATAACCAAGTTGTTTTAGAAACTAACTTTAAAAACTATTGTTTTTCAGTTTTCAAATTTTGGTTTGATTTTTTAAAAACATTGGTAAAAGGAGGTTATAATTTATACCATCATGTTTTAATTTTAAAATTTTGGTTAGATTTATAGTCGAAGAAGATGAAAATTATAGATAAGAAGTAGTAAAGGTGAAAACAACCATTAAAAAAATCACAAATACAAAGAGCTAACTTCTGTCTCTGTTGAGAACCTCAAGTTGGTCCCACTTCCACTTTATAATAATAAAAAAAAAATAATGGAAAAAAATAATCAAACATTTGAGCAGAGCCTACATAATATTATGCTAATAATAATTTAGATAAGATAGTAAATCAAACATTAACTTAGTAGTTTAGTCAAAGTTTGAGGCCCATTGGTTCTTGAAGTGACTTATAGCTATTTTGGCATTCCATTTTATATCTGATAAGTTTCGGCTGGCCTTTGAAACCATTCAATAAGTTGATAAACTTTTTTTGCCATTAAGTTTTTGCCCATACATTTCTAACATAAATGGAAAATGTTGTAAATTAACAAGTATCTTTGCAAGTAATTATTGAGATGGTCAAAATCACTTTTTCTCTCGTCAAAATGATAGTGTTTGGCATAAACTAAAAATTGATTTTAGAAAAATATAAAATCATTTTAAATTGATTTAAAAATAAGTAAGTGATATGTGACTTTTGTTAGGGATTTTCTCAAAAATAGAAGTATTTGACAAACTATTTACACTCCATAAAACAAAATTACTAAAAGATAAAATTTATTACACTTGATTTTTCTATGAAGCGTAGATATTTTGTCAAACATTCTATTTTTTATAATTTTTCCTTTTATATAATTATGTAATTACGGGTCAATCACTCAAAAAATGACCATCTAATTTAAATTTTGTGTAAAAATATTGTTGGTTAGTAGTATGTGAATTTTTTTTTTTTTTAAACTTAAATCTATCTCATCCACATTTTTACAATTGATCTTTCGTACGTATAATGACTAAATTCTTAGCCAATTTCCAAATATAAAAATAACATTTTTTAGTTCTTAAAATTTGGTTTGGTTTTTTTAAACTATGGATAACTTTAACAAATAAAAACAAATACACAATGGTTATTAGATGGATGCTTAATTTTTCGACTAATCAAAGGAACACATAGTTTAATTGTGAAGTCCTCACTAATTCATATAATATATATATATATATATATATATATATATATATATATATCTGTAAGAAAATAGAAGGAAGGGCCACGAAGCTGTCTTAGAATTAATTAAAAATCAATCACACAAATTGTTAAGGTATCATCTTTAAACTTCCATTTTGAAAATTAATGAGATAGATAGCAAATTAATCAAAAATTGATCTACTTTATTTTTAAATATAGTAAAATAAATTAAAATATATATAAATATAATAAAATATTATTGTCAATTTGTAATAGATTCTACGGTAGATTTTGATAAACTACTACACACATATAATTTATCGAATATGATATTTTGTTATATTTGTAAATATTTTTTAGCAATTTTTTCGTTAATTAAAAAATATATATTTTTTTTTTTTATGATACAGTGTGTGAGAACGGTGGTAGTGTTACACACATCATTCCTAGTAAACTCAATATGCCATAGATTGGGAAAGCAACAATGGAAGACTCGTGATTTGTCATTAAACAATGGGTAAGTTAATTTGTTTTGCCTTCTCTATTAATATATACTTGTTTGGTTTCACTACTTTTCAATAGTTTGATGGATAAGCTTGTGTGAGACTTGTAATTTTATTAGATAAAACCTACTCGTCAACTAACCATAAATCAAAGGTGAATTCTCAATTATGACTTTCTTTCAAAATTCTCCATTTTGTTTAGTTCCACTTTTTTTTTTAAGTACTTCACGTACATGTATACATACAATATTTTCAAACAACACTTGAATACCTAAATAATGTTGTACTTATATGTTAAGAAAATACATTTTGAATGATACACAAATAACCTAAGTTTAGTGAATTAAAATATAGGCTCTTTAATTTCTAAGGTTGAGACTCTCTCTCAATTGTAAATTACCTTTCTTTTCTCAATCAAAAGTCCCTACACTTGGGAAACCCTAGCTTAAGCTCCGGTATCTAAGAATGCGAAGACTTCTCAAGTATAAGAAATTGCACTTCAAAATAGTACAAAGATAACGATCTCATTTCAAGTGGAAGCCTTAGACTTAATTGAAATAATTATACGTTAAATTTTACATCATATTTAGTCAAATAAGATCCAAAGGAGTTACTTCTAAATTAATAAATCTATGAAAATTTATGATTTAAATTAATACGGACCACTGATGATGTTACGTAATACAATTTTTAAATTAAAAGAAGTATAAAATTATATATATATATTTTTTATATAAATGATGATGTTATTTATGAATAGTAGTTTATAATAATTGATATTGGTTGTATTAGGTGGATGAGTTTGGTAAGTTTTGGAGAAAGTTGGCATAATAACCACCATGCCTTTCCTTATTCAGCCAGACATGGATTTGAATGGTGGCAGATTGACTTTAGTTGGTATGCTATTAACTTTCTTCAAGCCATTGGGGTTGCCACTAATGTCAAATTGCCCCCTCCCAAAGTCATACATAACCAAAACCCTCTATAA

mRNA sequence

CCTAATTGACAATGGATGTATCAATGGAACCAAGTGAAATAGAAAGATCTCCAACATCATTCGGGAAGGAAGAATGGGCCGAGATACACAAAGGAATGGCTTTTATAATTTTCCTCTTGCATCTCCTTTCCATTTTTGCACCCTTTCAATTTAGTTGGAGTGCATTATGGGTTGCTGTGGCATTACATGTTATCACGGGTCTCTTTGGTATGAATCTTTCATATCACAGAAATCTTTCGCACAAGAGTTTTAAGCTTCCAAATTGGCTCGAGTACTTGTTTGCTTACTGTGGAGTTCAAGCACTTGAGGGAGATCCAATTGATTGGGTGAGTACGCATAGATATTACCACCAAGTTGCTGATACTAATAAAGATCCACAACGTCTTGAGCAAAGATTTTTGTTGCTTTCAAATTACTATGGATCAAATAAAAGAGTTAATTCGAGGTACTTTGCTATTTTTAAAGAGATAGAAAAGAACGTGTTTTTAATGGTTAGGAAGAAAGTGAGACGAGATAATGTTGAGGACTTGGAGAAGCAAAGTTTCTACAAATTCATTCACAAAACGTATATTATTCATCCAATTGCTCTTGCAATCTTACTATATGCAGTTGGAGGAATGCCTTTTCTTATATGGGGAATGGTAAAATTGGGAAAGCAACAATGGAAGACTCGTGATTTGTCATTAAACAATGGGTGGATGAGTTTGGTAAGTTTTGGAGAAAGTTGGCATAATAACCACCATGCCTTTCCTTATTCAGCCAGACATGGATTTGAATGGTGGCAGATTGACTTTAGTTGGTATGCTATTAACTTTCTTCAAGCCATTGGGGTTGCCACTAATGTCAAATTGCCCCCTCCCAAAGTCATACATAACCAAAACCCTCTATAA

Coding sequence (CDS)

ATGGATGTATCAATGGAACCAAGTGAAATAGAAAGATCTCCAACATCATTCGGGAAGGAAGAATGGGCCGAGATACACAAAGGAATGGCTTTTATAATTTTCCTCTTGCATCTCCTTTCCATTTTTGCACCCTTTCAATTTAGTTGGAGTGCATTATGGGTTGCTGTGGCATTACATGTTATCACGGGTCTCTTTGGTATGAATCTTTCATATCACAGAAATCTTTCGCACAAGAGTTTTAAGCTTCCAAATTGGCTCGAGTACTTGTTTGCTTACTGTGGAGTTCAAGCACTTGAGGGAGATCCAATTGATTGGGTGAGTACGCATAGATATTACCACCAAGTTGCTGATACTAATAAAGATCCACAACGTCTTGAGCAAAGATTTTTGTTGCTTTCAAATTACTATGGATCAAATAAAAGAGTTAATTCGAGGTACTTTGCTATTTTTAAAGAGATAGAAAAGAACGTGTTTTTAATGGTTAGGAAGAAAGTGAGACGAGATAATGTTGAGGACTTGGAGAAGCAAAGTTTCTACAAATTCATTCACAAAACGTATATTATTCATCCAATTGCTCTTGCAATCTTACTATATGCAGTTGGAGGAATGCCTTTTCTTATATGGGGAATGGTAAAATTGGGAAAGCAACAATGGAAGACTCGTGATTTGTCATTAAACAATGGGTGGATGAGTTTGGTAAGTTTTGGAGAAAGTTGGCATAATAACCACCATGCCTTTCCTTATTCAGCCAGACATGGATTTGAATGGTGGCAGATTGACTTTAGTTGGTATGCTATTAACTTTCTTCAAGCCATTGGGGTTGCCACTAATGTCAAATTGCCCCCTCCCAAAGTCATACATAACCAAAACCCTCTATAA

Protein sequence

MDVSMEPSEIERSPTSFGKEEWAEIHKGMAFIIFLLHLLSIFAPFQFSWSALWVAVALHVITGLFGMNLSYHRNLSHKSFKLPNWLEYLFAYCGVQALEGDPIDWVSTHRYYHQVADTNKDPQRLEQRFLLLSNYYGSNKRVNSRYFAIFKEIEKNVFLMVRKKVRRDNVEDLEKQSFYKFIHKTYIIHPIALAILLYAVGGMPFLIWGMVKLGKQQWKTRDLSLNNGWMSLVSFGESWHNNHHAFPYSARHGFEWWQIDFSWYAINFLQAIGVATNVKLPPPKVIHNQNPL
Homology
BLAST of IVF0025894 vs. ExPASy Swiss-Prot
Match: Q949X0 (Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ADS3 PE=1 SV=2)

HSP 1 Score: 297.4 bits (760), Expect = 1.8e-79
Identity = 141/282 (50.00%), Postives = 186/282 (65.96%), Query Frame = 0

Query: 19  KEEWAEIHKGMAFIIFLLHLLSIFAPFQFSWSALWVAVALHVITGLFGMNLSYHRNLSHK 78
           + EW  +  G   ++  +HLLS+ APFQF+W A+ VA  L+++TGL G+ LS+HRNLSHK
Sbjct: 95  EREWKAMDFGAVAVVLSMHLLSLLAPFQFNWRAVSVAFGLYIVTGLLGITLSFHRNLSHK 154

Query: 79  SFKLPNWLEYLFAYCGVQALEGDPIDWVSTHRYYHQVADTNKDPQR-LEQRFLLLSNYYG 138
           +FKLP WLEYLFAYCG QAL+G+PIDWVSTHRY+HQ  D+++DP   L+  +    N+  
Sbjct: 155 AFKLPKWLEYLFAYCGAQALQGNPIDWVSTHRYHHQFCDSDRDPHSPLDGFWFSHMNWMF 214

Query: 139 SNKRVNSRYFAIFKEIEKNVFLMVRKKVRRDNVEDLEKQSFYKFIHKTYIIHPIALAILL 198
               +  R                      +NV DLEKQ FY+F+  TYI+HP+ALA+ L
Sbjct: 215 DTNTITQRC------------------GEPNNVGDLEKQPFYRFLRTTYILHPLALAVAL 274

Query: 199 YAVGGMPFLIWGM-VKL-----------------GKQQWKTRDLSLNNGWMSLVSFGESW 258
           YA+GG PF++WGM V++                 GKQ W T DLS NN W++ ++FGE W
Sbjct: 275 YAMGGFPFIVWGMGVRIVWVYHITWLVNSACHVWGKQAWNTGDLSKNNWWVAALAFGEGW 334

Query: 259 HNNHHAFPYSARHGFEWWQIDFSWYAINFLQAIGVATNVKLP 282
           HNNHHAF +SARHG EWWQ+D +WY + FLQAIG+AT+VKLP
Sbjct: 335 HNNHHAFEFSARHGLEWWQLDMTWYVVKFLQAIGLATDVKLP 358

BLAST of IVF0025894 vs. ExPASy Swiss-Prot
Match: O65797 (Delta-9 acyl-lipid desaturase 1 OS=Arabidopsis thaliana OX=3702 GN=ADS1 PE=2 SV=1)

HSP 1 Score: 259.6 bits (662), Expect = 4.2e-68
Identity = 131/297 (44.11%), Postives = 179/297 (60.27%), Query Frame = 0

Query: 3   VSMEPSEIERSPTSFGKEEWAEIHKGMAFIIFLLHLLSIFAPFQFSWSALWVAVALHVIT 62
           ++ + +E+ R   +  + +W  +    AF    +H L + APF F+W AL VA+ ++ + 
Sbjct: 15  MAADKAEMGRKKRAMWERKWKRLDIVKAFASLFVHFLCLLAPFNFTWPALRVALIVYTVG 74

Query: 63  GLFGMNLSYHRNLSHKSFKLPNWLEYLFAYCGVQALEGDPIDWVSTHRYYHQVADTNKDP 122
           GL G+ +SYHRNL+H+SFK+P WLEY FAYCG+ A++GDPIDWVSTHRY+HQ  D+++DP
Sbjct: 75  GL-GITVSYHRNLAHRSFKVPKWLEYFFAYCGLLAIQGDPIDWVSTHRYHHQFTDSDRDP 134

Query: 123 QRLEQRFLLLSNYYGSNKRVNSRYFAIFKEIEKNVFLMVRKKVRRDNVEDLEKQSFYKFI 182
               + F              S    +F     +   +V K  RR NVEDL++Q +YKF+
Sbjct: 135 HSPNEGFWF------------SHLLWLF-----DTGYLVEKCGRRTNVEDLKRQWYYKFL 194

Query: 183 HKTYIIHPIALAILLYAVGGMPFLIWGM---VKL---------------GKQQWKTRDLS 242
            +T + H +    LLY  GG+ FL WGM   V +               G + WKT D S
Sbjct: 195 QRTVLYHILTFGFLLYYFGGLSFLTWGMGIGVAMEHHVTCLINSLCHVWGSRTWKTNDTS 254

Query: 243 LNNGWMSLVSFGESWHNNHHAFPYSARHGFEWWQIDFSWYAINFLQAIGVATNVKLP 282
            N  W+S+ SFGESWHNNHHAF  SAR G EWWQID SWY + FL+ IG+AT+VKLP
Sbjct: 255 RNVWWLSVFSFGESWHNNHHAFESSARQGLEWWQIDISWYIVRFLEIIGLATDVKLP 293

BLAST of IVF0025894 vs. ExPASy Swiss-Prot
Match: Q9FV68 (Icosanoyl-CoA 5-desaturase (Fragment) OS=Limnanthes douglasii OX=28973 PE=1 SV=1)

HSP 1 Score: 253.4 bits (646), Expect = 3.0e-66
Identity = 129/291 (44.33%), Postives = 175/291 (60.14%), Query Frame = 0

Query: 10  IERSPTSFGKEEWAEIHKGMAFIIFLLHLLSIFAPFQFSWSALWVA-VALHVITGLFGMN 69
           +ER    F + +W       A  + +LH L+  APF FSW A W++ + L   +G+ G+ 
Sbjct: 69  LERKAKGFWRRKWNPRDIQNAVTLLVLHALAAMAPFYFSWDAFWISFILLGFASGVLGIT 128

Query: 70  LSYHRNLSHKSFKLPNWLEYLFAYCGVQALEGDPIDWVSTHRYYHQVADTNKDPQRLEQR 129
           L +HR L+H  FKLP  +EY FAYCG  AL+GDP++WVS HRY+HQ  DT +D     Q 
Sbjct: 129 LCFHRCLTHGGFKLPKLVEYFFAYCGSLALQGDPMEWVSNHRYHHQFVDTERDVHSPTQG 188

Query: 130 FLLLSNYYGSNKRVNSRYFAIFKEIEKNVFLMVRKKVRRDNVEDLEKQSFYKFIHKTYII 189
           F                +  I   ++K++F + ++  RR+NV DL+KQ+FY+F+ KTY+ 
Sbjct: 189 FW---------------FCHIGWVLDKDLF-VEKRGGRRNNVNDLKKQAFYRFLQKTYMY 248

Query: 190 HPIALAILLYAVGGMPFLIWGM------------------VKLGKQQWKTRDLSLNNGWM 249
           H +AL  LLY VGG P+++WGM                   K G + W T DLS NN ++
Sbjct: 249 HQLALIALLYYVGGFPYIVWGMGFRLVFMFHSTFAINSVCHKWGGRPWNTGDLSTNNMFV 308

Query: 250 SLVSFGESWHNNHHAFPYSARHGFEWWQIDFSWYAINFLQAIGVATNVKLP 282
           +L +FGE WHNNHHAF  SARHG EWW+ID +WY I  LQAIG+ATNVKLP
Sbjct: 309 ALCAFGEGWHNNHHAFEQSARHGLEWWEIDVTWYVIRTLQAIGLATNVKLP 343

BLAST of IVF0025894 vs. ExPASy Swiss-Prot
Match: Q9SID2 (Delta-9 acyl-lipid desaturase 2 OS=Arabidopsis thaliana OX=3702 GN=ADS2 PE=2 SV=2)

HSP 1 Score: 253.1 bits (645), Expect = 3.9e-66
Identity = 128/283 (45.23%), Postives = 168/283 (59.36%), Query Frame = 0

Query: 17  FGKEEWAEIHKGMAFIIFLLHLLSIFAPFQFSWSALWVAVALHVITGLFGMNLSYHRNLS 76
           F    W  +        F +H L++ APF F+WSALWV    + I GL G+ +SYHRNL+
Sbjct: 31  FWDRRWRRLDYVKFSASFTVHSLALLAPFYFTWSALWVTFLFYTIGGL-GITVSYHRNLA 90

Query: 77  HKSFKLPNWLEYLFAYCGVQALEGDPIDWVSTHRYYHQVADTNKDPQRLEQRFLLLSNYY 136
           H+SFK+P WLEYL AYC + A++GDPIDWVSTHRY+HQ  D+ +DP   ++ F       
Sbjct: 91  HRSFKVPKWLEYLLAYCALLAIQGDPIDWVSTHRYHHQFTDSERDPHSPKEGF------- 150

Query: 137 GSNKRVNSRYFAIFKEIEKNVFLMVRKKVRRDNVEDLEKQSFYKFIHKTYIIHPIALAIL 196
                    +F+    I  + +L V K  RR NVEDL++Q FY+F+ KT + H + L   
Sbjct: 151 ---------WFSHLLWIYDSAYL-VSKCGRRANVEDLKRQWFYRFLQKTVLFHILGLGFF 210

Query: 197 LYAVGGMPFLIWGMVK------------------LGKQQWKTRDLSLNNGWMSLVSFGES 256
           L+ +GGM F+ WGM                     G + WKT D S N  W+S+ SFGES
Sbjct: 211 LFYLGGMSFVTWGMGVGAALEVHVTCLINSLCHIWGTRTWKTNDTSRNVWWLSVFSFGES 270

Query: 257 WHNNHHAFPYSARHGFEWWQIDFSWYAINFLQAIGVATNVKLP 282
           WHNNHHAF  SAR G EWWQID SWY + F + IG+AT+VK+P
Sbjct: 271 WHNNHHAFESSARQGLEWWQIDISWYIVRFFEIIGLATDVKVP 295

BLAST of IVF0025894 vs. ExPASy Swiss-Prot
Match: P0DOW3 (Acyl-CoA 5-desaturase AL21 OS=Anemone leveillei OX=212809 GN=AL21 PE=1 SV=1)

HSP 1 Score: 248.4 bits (633), Expect = 9.7e-65
Identity = 119/264 (45.08%), Postives = 165/264 (62.50%), Query Frame = 0

Query: 36  LHLLSIFAPFQFSWSALWVAVALHVITGLFGMNLSYHRNLSHKSFKLPNWLEYLFAYCGV 95
           LH+L + APF FSWSA W+++ L+ + G+FG  LSYHRNL+H+SFKLP +LEY FAY G+
Sbjct: 51  LHVLCLSAPFTFSWSAFWLSLTLYAVCGVFGTTLSYHRNLTHRSFKLPKYLEYFFAYVGL 110

Query: 96  QALEGDPIDWVSTHRYYHQVADTNKDPQRLEQRFLLLSNYYGSNKRVNSRYFAIFKEIEK 155
            AL+GDP+ WVSTHRY+H+  DT  DP    + F     ++      +S+Y         
Sbjct: 111 HALQGDPVWWVSTHRYHHKFTDTYLDPHSPIEGFWFSHIFW----LFDSKY--------- 170

Query: 156 NVFLMVRKKVRRDNVEDLEKQSFYKFIHKTYIIHPIALAILLYAVGGMPFLIWGMV---- 215
               ++ +  R +N  DL KQS+Y+F+ +T++ H    A LLY  GG PF++WGM     
Sbjct: 171 ----ILEECGRYENAGDLLKQSYYRFLERTFVFHVYLQAALLYMFGGFPFIVWGMAVRTV 230

Query: 216 --------------KLGKQQWKTRDLSLNNGWMSLVSFGESWHNNHHAFPYSARHGFEWW 275
                         + G Q W T DLS NN ++++++ GE WHNNHHAF YSARHG EWW
Sbjct: 231 WGFHASWLVNSVCHRYGHQAWDTGDLSTNNWFIAMLTSGEGWHNNHHAFEYSARHGIEWW 290

Query: 276 QIDFSWYAINFLQAIGVATNVKLP 282
           QID +WY I  L+ +G+AT+VK+P
Sbjct: 291 QIDTTWYVIKLLEYLGLATDVKVP 297

BLAST of IVF0025894 vs. ExPASy TrEMBL
Match: A0A5D3DZT8 (Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold403G001150 PE=3 SV=1)

HSP 1 Score: 433.7 bits (1114), Expect = 6.0e-118
Identity = 212/212 (100.00%), Postives = 212/212 (100.00%), Query Frame = 0

Query: 1   MDVSMEPSEIERSPTSFGKEEWAEIHKGMAFIIFLLHLLSIFAPFQFSWSALWVAVALHV 60
           MDVSMEPSEIERSPTSFGKEEWAEIHKGMAFIIFLLHLLSIFAPFQFSWSALWVAVALHV
Sbjct: 1   MDVSMEPSEIERSPTSFGKEEWAEIHKGMAFIIFLLHLLSIFAPFQFSWSALWVAVALHV 60

Query: 61  ITGLFGMNLSYHRNLSHKSFKLPNWLEYLFAYCGVQALEGDPIDWVSTHRYYHQVADTNK 120
           ITGLFGMNLSYHRNLSHKSFKLPNWLEYLFAYCGVQALEGDPIDWVSTHRYYHQVADTNK
Sbjct: 61  ITGLFGMNLSYHRNLSHKSFKLPNWLEYLFAYCGVQALEGDPIDWVSTHRYYHQVADTNK 120

Query: 121 DPQRLEQRFLLLSNYYGSNKRVNSRYFAIFKEIEKNVFLMVRKKVRRDNVEDLEKQSFYK 180
           DPQRLEQRFLLLSNYYGSNKRVNSRYFAIFKEIEKNVFLMVRKKVRRDNVEDLEKQSFYK
Sbjct: 121 DPQRLEQRFLLLSNYYGSNKRVNSRYFAIFKEIEKNVFLMVRKKVRRDNVEDLEKQSFYK 180

Query: 181 FIHKTYIIHPIALAILLYAVGGMPFLIWGMVK 213
           FIHKTYIIHPIALAILLYAVGGMPFLIWGMVK
Sbjct: 181 FIHKTYIIHPIALAILLYAVGGMPFLIWGMVK 212

BLAST of IVF0025894 vs. ExPASy TrEMBL
Match: A0A6J1JGI0 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111485493 PE=3 SV=1)

HSP 1 Score: 419.9 bits (1078), Expect = 9.0e-114
Identity = 202/302 (66.89%), Postives = 229/302 (75.83%), Query Frame = 0

Query: 1   MDVSMEPSEIERSPTSFGKEEWAEIHKGMAFIIFLLHLLSIFAPFQFSWSALWVAVALHV 60
           MDV +EP +  + P S GKEEWA+I K  A  IFL+H+L IFAPFQ +  A+ VA+ALH 
Sbjct: 1   MDVPIEPMDTAKPPRSSGKEEWAKIDKQKAIFIFLMHVLCIFAPFQVNSRAIRVALALHF 60

Query: 61  ITGLFGMNLSYHRNLSHKSFKLPNWLEYLFAYCGVQALEGDPIDWVSTHRYYHQVADTNK 120
           ITG FG+ LSYHRNLSH+SFKLP W EYLFA CGV  L GDPI+WVS HRY+HQVADT K
Sbjct: 61  ITGFFGITLSYHRNLSHRSFKLPKWFEYLFALCGVHTLLGDPINWVSMHRYHHQVADTEK 120

Query: 121 DPQRLEQRFLLLSNYYGSNKRVNSRYFAIFKEIEKNVFLMVRKKVRRDNVEDLEKQSFYK 180
           DPQR  +RFL LSNY+ S KRV  RY  I + IEKN+FLM+RK V+RDNV DLE QSFYK
Sbjct: 121 DPQRTPRRFLWLSNYFASTKRVGPRYLCILQGIEKNIFLMIRKFVKRDNVGDLENQSFYK 180

Query: 181 FIHKTYIIHPIALAILLYAVGGMPFLIWGM------------------VKLGKQQWKTRD 240
           FIHKTY++HPI LAILLY VGGMPFL+WGM                   + GK+ WKT D
Sbjct: 181 FIHKTYLLHPIGLAILLYVVGGMPFLVWGMGVRTVLVLHLSLMVNTICHRFGKRSWKTND 240

Query: 241 LSLNNGWMSLVSFGESWHNNHHAFPYSARHGFEWWQIDFSWYAINFLQAIGVATNVKLPP 285
            S NNG M L+SFGE WHNNHHAFPYSARHGFEWWQID SWYAI FLQAIGVAT+VKLPP
Sbjct: 241 SSRNNGLMGLISFGEGWHNNHHAFPYSARHGFEWWQIDTSWYAIRFLQAIGVATDVKLPP 300

BLAST of IVF0025894 vs. ExPASy TrEMBL
Match: A0A6J1H9D0 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111461702 PE=3 SV=1)

HSP 1 Score: 406.8 bits (1044), Expect = 7.9e-110
Identity = 195/287 (67.94%), Postives = 221/287 (77.00%), Query Frame = 0

Query: 16  SFGKEEWAEIHKGMAFIIFLLHLLSIFAPFQFSWSALWVAVALHVITGLFGMNLSYHRNL 75
           S GKEEWA+I K  A  IFL+H+L IFAPFQ +   L +A+ALH ITG FG+ LSYHRNL
Sbjct: 9   SSGKEEWAKIDKQKAIFIFLMHVLCIFAPFQVNSRVLRIALALHFITGFFGITLSYHRNL 68

Query: 76  SHKSFKLPNWLEYLFAYCGVQALEGDPIDWVSTHRYYHQVADTNKDPQRLEQRFLLLSNY 135
           SH+SFKLP WLEYLFA CG   L GDPI+WVS HRY+HQVADT KDPQR  +RFL LSNY
Sbjct: 69  SHRSFKLPKWLEYLFALCGAHTLLGDPINWVSMHRYHHQVADTEKDPQRTPRRFLWLSNY 128

Query: 136 YGSNKRVNSRYFAIFKEIEKNVFLMVRKKVRRDNVEDLEKQSFYKFIHKTYIIHPIALAI 195
           + S KRV  RY  I + IEKN+FLM+R+ V+RDNV DLE QSFYKFIHKTY++HPI LAI
Sbjct: 129 FASTKRVGPRYLFILEGIEKNIFLMIREFVKRDNVGDLENQSFYKFIHKTYLLHPIGLAI 188

Query: 196 LLYAVGGMPFLIWGM------------------VKLGKQQWKTRDLSLNNGWMSLVSFGE 255
           LLY VGGMPFL+WGM                   + GK+QWKT D S NNG MSL+SFGE
Sbjct: 189 LLYVVGGMPFLVWGMGVRTVLVLHLSLMVNTICHRFGKRQWKTNDSSRNNGLMSLISFGE 248

Query: 256 SWHNNHHAFPYSARHGFEWWQIDFSWYAINFLQAIGVATNVKLPPPK 285
            WHNNHHAFPYSARHGFEWWQ+D SWYAI FLQAIGVAT+VKLPPP+
Sbjct: 249 GWHNNHHAFPYSARHGFEWWQVDTSWYAIRFLQAIGVATDVKLPPPQ 295

BLAST of IVF0025894 vs. ExPASy TrEMBL
Match: A0A6J1DHZ7 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111021091 PE=3 SV=1)

HSP 1 Score: 364.4 bits (934), Expect = 4.5e-97
Identity = 175/300 (58.33%), Postives = 216/300 (72.00%), Query Frame = 0

Query: 6   EPSEIERSPTSFGK-EEWAEIHKGMAFIIFLLHLLSIFAPFQFSWSALWVAVALHVITGL 65
           EP E+   P SFGK ++W ++ K +A  +F +HLLS+FAPF F+W A W+A AL+V TGL
Sbjct: 27  EPKEMAIPPKSFGKRKKWTKLDKDVACSLFFVHLLSMFAPFYFNWRAFWIAFALYVATGL 86

Query: 66  FGMNLSYHRNLSHKSFKLPNWLEYLFAYCGVQALEGDPIDWVSTHRYYHQVADTNKDPQR 125
           FG+++SYHRNL+HKSF LP WLEYLFAYCG  AL+GDPIDWV+THR +H+  DT +DP  
Sbjct: 87  FGISVSYHRNLAHKSFTLPKWLEYLFAYCGAHALQGDPIDWVNTHRCHHRFVDTERDPHS 146

Query: 126 LEQRFLL-----LSNYYGSNKRVNSRYFAIFKEIEKNVFLMVRKKVRRDNVEDLEKQSFY 185
             Q F       L + Y   KRV  +YF  F+++E+N+F+M  K  R DNV DLEKQ+FY
Sbjct: 147 PIQGFWFSHITWLLDSYSLTKRVCPKYFTDFQKVERNIFVMFSKHGRPDNVGDLEKQTFY 206

Query: 186 KFIHKTYIIHPIALAILLYAVGGMPFLIWGM-VKL-----------------GKQQWKTR 245
           +FIHKTY +HPI LAI+LYA GG PFLIWGM V++                 GKQ W+T 
Sbjct: 207 RFIHKTYFLHPIILAIILYAYGGAPFLIWGMGVRIVVVLHITCMINSVCHIWGKQPWRTG 266

Query: 246 DLSLNNGWMSLVSFGESWHNNHHAFPYSARHGFEWWQIDFSWYAINFLQAIGVATNVKLP 282
           DLS NN W+SLVSFGE WHNNHHAF YSARHG EWWQIDF WY + FL+AIG+ATNVK+P
Sbjct: 267 DLSRNNWWVSLVSFGEGWHNNHHAFEYSARHGIEWWQIDFGWYVVMFLEAIGIATNVKVP 326

BLAST of IVF0025894 vs. ExPASy TrEMBL
Match: A0A6J1DJP0 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111021101 PE=3 SV=1)

HSP 1 Score: 363.2 bits (931), Expect = 1.0e-96
Identity = 169/306 (55.23%), Postives = 221/306 (72.22%), Query Frame = 0

Query: 6   EPSEIERSPTSFGKEEWAEIHKGMAFIIFLLHLLSIFAPFQFSWSALWVAVALHVITGLF 65
           EP E+ + P  + + +W ++ K +A  +FL+HLL I APFQF+WSA WVA  L+++TGLF
Sbjct: 7   EPKEMMKPPRPYERRKWTKLDKDIACSLFLVHLLCILAPFQFNWSAFWVAFVLYIVTGLF 66

Query: 66  GMNLSYHRNLSHKSFKLPNWLEYLFAYCGVQALEGDPIDWVSTHRYYHQVADTNKDPQRL 125
           GM LSYHRNL+HKSFKLP WLEYLFAYCGVQAL+GDP+DWVSTHR +HQ  DT +DP   
Sbjct: 67  GMTLSYHRNLAHKSFKLPKWLEYLFAYCGVQALQGDPMDWVSTHRCHHQFVDTERDPHSP 126

Query: 126 EQRF-----LLLSNYYGSNKRVNSRYFAIFKEIEKNVFLMVRKKVRRDNVEDLEKQSFYK 185
            + F     L L + Y   K+V  +YF+ F+++E+N+F++  K  + +NV DLEKQ+FY+
Sbjct: 127 IRGFWFSHILWLFDSYNLTKKVCPKYFSNFQKLERNIFVLFSKHGKPENVADLEKQAFYR 186

Query: 186 FIHKTYIIHPIALAILLYAVGGMPFLIWGM-VKL-----------------GKQQWKTRD 245
           FIH+TY++HPI LA++LY VGG+PFL+WGM V++                 GKQ W T D
Sbjct: 187 FIHQTYLLHPIVLAVILYVVGGIPFLVWGMAVRIVVVLHFTLMVNSVCHIWGKQSWTTGD 246

Query: 246 LSLNNGWMSLVSFGESWHNNHHAFPYSARHGFEWWQIDFSWYAINFLQAIGVATNVKLPP 289
           +S NN W+SL++FGE WHNNHHAF YSA+ G EWWQ+DF WY I FLQAIGVAT+VKLP 
Sbjct: 247 MSKNNWWVSLITFGEGWHNNHHAFEYSAKFGLEWWQLDFGWYMILFLQAIGVATHVKLPS 306

BLAST of IVF0025894 vs. NCBI nr
Match: XP_038879870.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 472 bits (1215), Expect = 1.01e-165
Identity = 231/308 (75.00%), Postives = 255/308 (82.79%), Query Frame = 0

Query: 1   MDVSMEPSEIERSPTSFGKEEWAEIHKGMAFIIFLLHLLSIFAPFQFSWSALWVAVALHV 60
           MDVS+EP E+E+   S GKEEW EI K MAFIIFLLH+L IFAPFQF+WSALWVA  L+ 
Sbjct: 1   MDVSIEPREMEKPLKSSGKEEWIEIDKEMAFIIFLLHVLCIFAPFQFNWSALWVATMLYF 60

Query: 61  ITGLFGMNLSYHRNLSHKSFKLPNWLEYLFAYCGVQALEGDPIDWVSTHRYYHQVADTNK 120
           I+GLFG++LSYHRNL+H+SFKLP WLEYLFAYCGVQALEG+PIDWVS HRY+H+VADT+K
Sbjct: 61  ISGLFGLSLSYHRNLAHRSFKLPKWLEYLFAYCGVQALEGNPIDWVSMHRYHHEVADTDK 120

Query: 121 DPQRLEQRFLLLSNYYGSNKRVNS-RYFAIFKEIEKNVFLMVRKKVRRDNVEDLEKQSFY 180
           DPQR +QRF    NYY S KRVN  RYF IF+EIEKN+FLMVRK+VRRDNV DLEKQ FY
Sbjct: 121 DPQRFDQRFSWFPNYYASTKRVNRPRYFVIFQEIEKNIFLMVRKQVRRDNVRDLEKQIFY 180

Query: 181 KFIHKTYIIHPIALAILLYAVGGMPFLIWGM-----VKL-------------GKQQWKTR 240
           KFIHKTY++HPIALAILLY VGGMPFLIWGM     V L             GKQQWKTR
Sbjct: 181 KFIHKTYLLHPIALAILLYVVGGMPFLIWGMGVRNVVVLHTTLMVNSICHGSGKQQWKTR 240

Query: 241 DLSLNNGWMSLVSFGESWHNNHHAFPYSARHGFEWWQIDFSWYAINFLQAIGVATNVKLP 289
           DLSLN GW+SL+SFGESWHNNHHAFPYSARHGF+WWQIDFSWY INFLQ IGVATNVKL 
Sbjct: 241 DLSLNVGWVSLISFGESWHNNHHAFPYSARHGFKWWQIDFSWYVINFLQVIGVATNVKLA 300

BLAST of IVF0025894 vs. NCBI nr
Match: TYK28760.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase [Cucumis melo var. makuwa])

HSP 1 Score: 432 bits (1110), Expect = 2.49e-151
Identity = 212/212 (100.00%), Postives = 212/212 (100.00%), Query Frame = 0

Query: 1   MDVSMEPSEIERSPTSFGKEEWAEIHKGMAFIIFLLHLLSIFAPFQFSWSALWVAVALHV 60
           MDVSMEPSEIERSPTSFGKEEWAEIHKGMAFIIFLLHLLSIFAPFQFSWSALWVAVALHV
Sbjct: 1   MDVSMEPSEIERSPTSFGKEEWAEIHKGMAFIIFLLHLLSIFAPFQFSWSALWVAVALHV 60

Query: 61  ITGLFGMNLSYHRNLSHKSFKLPNWLEYLFAYCGVQALEGDPIDWVSTHRYYHQVADTNK 120
           ITGLFGMNLSYHRNLSHKSFKLPNWLEYLFAYCGVQALEGDPIDWVSTHRYYHQVADTNK
Sbjct: 61  ITGLFGMNLSYHRNLSHKSFKLPNWLEYLFAYCGVQALEGDPIDWVSTHRYYHQVADTNK 120

Query: 121 DPQRLEQRFLLLSNYYGSNKRVNSRYFAIFKEIEKNVFLMVRKKVRRDNVEDLEKQSFYK 180
           DPQRLEQRFLLLSNYYGSNKRVNSRYFAIFKEIEKNVFLMVRKKVRRDNVEDLEKQSFYK
Sbjct: 121 DPQRLEQRFLLLSNYYGSNKRVNSRYFAIFKEIEKNVFLMVRKKVRRDNVEDLEKQSFYK 180

Query: 181 FIHKTYIIHPIALAILLYAVGGMPFLIWGMVK 212
           FIHKTYIIHPIALAILLYAVGGMPFLIWGMVK
Sbjct: 181 FIHKTYIIHPIALAILLYAVGGMPFLIWGMVK 212

BLAST of IVF0025894 vs. NCBI nr
Match: XP_022988176.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 417 bits (1071), Expect = 9.60e-144
Identity = 202/302 (66.89%), Postives = 229/302 (75.83%), Query Frame = 0

Query: 1   MDVSMEPSEIERSPTSFGKEEWAEIHKGMAFIIFLLHLLSIFAPFQFSWSALWVAVALHV 60
           MDV +EP +  + P S GKEEWA+I K  A  IFL+H+L IFAPFQ +  A+ VA+ALH 
Sbjct: 1   MDVPIEPMDTAKPPRSSGKEEWAKIDKQKAIFIFLMHVLCIFAPFQVNSRAIRVALALHF 60

Query: 61  ITGLFGMNLSYHRNLSHKSFKLPNWLEYLFAYCGVQALEGDPIDWVSTHRYYHQVADTNK 120
           ITG FG+ LSYHRNLSH+SFKLP W EYLFA CGV  L GDPI+WVS HRY+HQVADT K
Sbjct: 61  ITGFFGITLSYHRNLSHRSFKLPKWFEYLFALCGVHTLLGDPINWVSMHRYHHQVADTEK 120

Query: 121 DPQRLEQRFLLLSNYYGSNKRVNSRYFAIFKEIEKNVFLMVRKKVRRDNVEDLEKQSFYK 180
           DPQR  +RFL LSNY+ S KRV  RY  I + IEKN+FLM+RK V+RDNV DLE QSFYK
Sbjct: 121 DPQRTPRRFLWLSNYFASTKRVGPRYLCILQGIEKNIFLMIRKFVKRDNVGDLENQSFYK 180

Query: 181 FIHKTYIIHPIALAILLYAVGGMPFLIWGMV------------------KLGKQQWKTRD 240
           FIHKTY++HPI LAILLY VGGMPFL+WGM                   + GK+ WKT D
Sbjct: 181 FIHKTYLLHPIGLAILLYVVGGMPFLVWGMGVRTVLVLHLSLMVNTICHRFGKRSWKTND 240

Query: 241 LSLNNGWMSLVSFGESWHNNHHAFPYSARHGFEWWQIDFSWYAINFLQAIGVATNVKLPP 284
            S NNG M L+SFGE WHNNHHAFPYSARHGFEWWQID SWYAI FLQAIGVAT+VKLPP
Sbjct: 241 SSRNNGLMGLISFGEGWHNNHHAFPYSARHGFEWWQIDTSWYAIRFLQAIGVATDVKLPP 300

BLAST of IVF0025894 vs. NCBI nr
Match: XP_023533044.1 (acyl-CoA C20 Delta5-desaturase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 408 bits (1049), Expect = 3.38e-139
Identity = 198/300 (66.00%), Postives = 227/300 (75.67%), Query Frame = 0

Query: 3   VSMEPSEIERSPTSFGKEEWAEIHKGMAFIIFLLHLLSIFAPFQFSWSALWVAVALHVIT 62
           V +EP +    P S GKEEWA+I K  A  IFL+H+L IFAPFQ +  AL +A+ALH IT
Sbjct: 83  VPLEPMDTPNPPRSSGKEEWAKIDKQKAIFIFLMHVLCIFAPFQVNSRALQIALALHFIT 142

Query: 63  GLFGMNLSYHRNLSHKSFKLPNWLEYLFAYCGVQALEGDPIDWVSTHRYYHQVADTNKDP 122
           G FG+ LSYHRNLSH+SFKLP WLEYLFA CG   L GDPI+WVS HRY+HQVADT KDP
Sbjct: 143 GFFGVTLSYHRNLSHRSFKLPKWLEYLFALCGAHTLLGDPINWVSMHRYHHQVADTEKDP 202

Query: 123 QRLEQRFLLLSNYYGSNKRVNSRYFAIFKEIEKNVFLMVRKKVRRDNVEDLEKQSFYKFI 182
           QR  +RFL LSNY+GS KRV  RY  I + IEKN+FLM+R+ V+RDNV DLE QSFYKFI
Sbjct: 203 QRTPRRFLWLSNYFGSTKRVGPRYLCILEGIEKNIFLMIREFVKRDNVGDLENQSFYKFI 262

Query: 183 HKTYIIHPIALAILLYAVGGMPFLIWGMV------------------KLGKQQWKTRDLS 242
           HKTY++HPI LAILLY VGGMP L+WGM                   + GK+QWKT D S
Sbjct: 263 HKTYLLHPIGLAILLYVVGGMPSLVWGMGVRTVLVLHLSLMVNTICHRFGKRQWKTNDSS 322

Query: 243 LNNGWMSLVSFGESWHNNHHAFPYSARHGFEWWQIDFSWYAINFLQAIGVATNVKLPPPK 284
            NNG MSL+SFGE WHNNHHAF YSARHGFEWWQ+D SWYAI FLQAIGVA++VKLPPP+
Sbjct: 323 RNNGLMSLISFGEGWHNNHHAFLYSARHGFEWWQVDTSWYAIRFLQAIGVASDVKLPPPQ 382

BLAST of IVF0025894 vs. NCBI nr
Match: XP_022961086.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 404 bits (1038), Expect = 7.84e-139
Identity = 195/287 (67.94%), Postives = 221/287 (77.00%), Query Frame = 0

Query: 16  SFGKEEWAEIHKGMAFIIFLLHLLSIFAPFQFSWSALWVAVALHVITGLFGMNLSYHRNL 75
           S GKEEWA+I K  A  IFL+H+L IFAPFQ +   L +A+ALH ITG FG+ LSYHRNL
Sbjct: 9   SSGKEEWAKIDKQKAIFIFLMHVLCIFAPFQVNSRVLRIALALHFITGFFGITLSYHRNL 68

Query: 76  SHKSFKLPNWLEYLFAYCGVQALEGDPIDWVSTHRYYHQVADTNKDPQRLEQRFLLLSNY 135
           SH+SFKLP WLEYLFA CG   L GDPI+WVS HRY+HQVADT KDPQR  +RFL LSNY
Sbjct: 69  SHRSFKLPKWLEYLFALCGAHTLLGDPINWVSMHRYHHQVADTEKDPQRTPRRFLWLSNY 128

Query: 136 YGSNKRVNSRYFAIFKEIEKNVFLMVRKKVRRDNVEDLEKQSFYKFIHKTYIIHPIALAI 195
           + S KRV  RY  I + IEKN+FLM+R+ V+RDNV DLE QSFYKFIHKTY++HPI LAI
Sbjct: 129 FASTKRVGPRYLFILEGIEKNIFLMIREFVKRDNVGDLENQSFYKFIHKTYLLHPIGLAI 188

Query: 196 LLYAVGGMPFLIWGMV------------------KLGKQQWKTRDLSLNNGWMSLVSFGE 255
           LLY VGGMPFL+WGM                   + GK+QWKT D S NNG MSL+SFGE
Sbjct: 189 LLYVVGGMPFLVWGMGVRTVLVLHLSLMVNTICHRFGKRQWKTNDSSRNNGLMSLISFGE 248

Query: 256 SWHNNHHAFPYSARHGFEWWQIDFSWYAINFLQAIGVATNVKLPPPK 284
            WHNNHHAFPYSARHGFEWWQ+D SWYAI FLQAIGVAT+VKLPPP+
Sbjct: 249 GWHNNHHAFPYSARHGFEWWQVDTSWYAIRFLQAIGVATDVKLPPPQ 295

BLAST of IVF0025894 vs. TAIR 10
Match: AT3G15850.1 (fatty acid desaturase 5 )

HSP 1 Score: 297.4 bits (760), Expect = 1.3e-80
Identity = 141/282 (50.00%), Postives = 186/282 (65.96%), Query Frame = 0

Query: 19  KEEWAEIHKGMAFIIFLLHLLSIFAPFQFSWSALWVAVALHVITGLFGMNLSYHRNLSHK 78
           + EW  +  G   ++  +HLLS+ APFQF+W A+ VA  L+++TGL G+ LS+HRNLSHK
Sbjct: 95  EREWKAMDFGAVAVVLSMHLLSLLAPFQFNWRAVSVAFGLYIVTGLLGITLSFHRNLSHK 154

Query: 79  SFKLPNWLEYLFAYCGVQALEGDPIDWVSTHRYYHQVADTNKDPQR-LEQRFLLLSNYYG 138
           +FKLP WLEYLFAYCG QAL+G+PIDWVSTHRY+HQ  D+++DP   L+  +    N+  
Sbjct: 155 AFKLPKWLEYLFAYCGAQALQGNPIDWVSTHRYHHQFCDSDRDPHSPLDGFWFSHMNWMF 214

Query: 139 SNKRVNSRYFAIFKEIEKNVFLMVRKKVRRDNVEDLEKQSFYKFIHKTYIIHPIALAILL 198
               +  R                      +NV DLEKQ FY+F+  TYI+HP+ALA+ L
Sbjct: 215 DTNTITQRC------------------GEPNNVGDLEKQPFYRFLRTTYILHPLALAVAL 274

Query: 199 YAVGGMPFLIWGM-VKL-----------------GKQQWKTRDLSLNNGWMSLVSFGESW 258
           YA+GG PF++WGM V++                 GKQ W T DLS NN W++ ++FGE W
Sbjct: 275 YAMGGFPFIVWGMGVRIVWVYHITWLVNSACHVWGKQAWNTGDLSKNNWWVAALAFGEGW 334

Query: 259 HNNHHAFPYSARHGFEWWQIDFSWYAINFLQAIGVATNVKLP 282
           HNNHHAF +SARHG EWWQ+D +WY + FLQAIG+AT+VKLP
Sbjct: 335 HNNHHAFEFSARHGLEWWQLDMTWYVVKFLQAIGLATDVKLP 358

BLAST of IVF0025894 vs. TAIR 10
Match: AT1G06080.1 (delta 9 desaturase 1 )

HSP 1 Score: 259.6 bits (662), Expect = 3.0e-69
Identity = 131/297 (44.11%), Postives = 179/297 (60.27%), Query Frame = 0

Query: 3   VSMEPSEIERSPTSFGKEEWAEIHKGMAFIIFLLHLLSIFAPFQFSWSALWVAVALHVIT 62
           ++ + +E+ R   +  + +W  +    AF    +H L + APF F+W AL VA+ ++ + 
Sbjct: 15  MAADKAEMGRKKRAMWERKWKRLDIVKAFASLFVHFLCLLAPFNFTWPALRVALIVYTVG 74

Query: 63  GLFGMNLSYHRNLSHKSFKLPNWLEYLFAYCGVQALEGDPIDWVSTHRYYHQVADTNKDP 122
           GL G+ +SYHRNL+H+SFK+P WLEY FAYCG+ A++GDPIDWVSTHRY+HQ  D+++DP
Sbjct: 75  GL-GITVSYHRNLAHRSFKVPKWLEYFFAYCGLLAIQGDPIDWVSTHRYHHQFTDSDRDP 134

Query: 123 QRLEQRFLLLSNYYGSNKRVNSRYFAIFKEIEKNVFLMVRKKVRRDNVEDLEKQSFYKFI 182
               + F              S    +F     +   +V K  RR NVEDL++Q +YKF+
Sbjct: 135 HSPNEGFWF------------SHLLWLF-----DTGYLVEKCGRRTNVEDLKRQWYYKFL 194

Query: 183 HKTYIIHPIALAILLYAVGGMPFLIWGM---VKL---------------GKQQWKTRDLS 242
            +T + H +    LLY  GG+ FL WGM   V +               G + WKT D S
Sbjct: 195 QRTVLYHILTFGFLLYYFGGLSFLTWGMGIGVAMEHHVTCLINSLCHVWGSRTWKTNDTS 254

Query: 243 LNNGWMSLVSFGESWHNNHHAFPYSARHGFEWWQIDFSWYAINFLQAIGVATNVKLP 282
            N  W+S+ SFGESWHNNHHAF  SAR G EWWQID SWY + FL+ IG+AT+VKLP
Sbjct: 255 RNVWWLSVFSFGESWHNNHHAFESSARQGLEWWQIDISWYIVRFLEIIGLATDVKLP 293

BLAST of IVF0025894 vs. TAIR 10
Match: AT2G31360.1 (16:0delta9 desaturase 2 )

HSP 1 Score: 253.1 bits (645), Expect = 2.8e-67
Identity = 128/283 (45.23%), Postives = 168/283 (59.36%), Query Frame = 0

Query: 17  FGKEEWAEIHKGMAFIIFLLHLLSIFAPFQFSWSALWVAVALHVITGLFGMNLSYHRNLS 76
           F    W  +        F +H L++ APF F+WSALWV    + I GL G+ +SYHRNL+
Sbjct: 31  FWDRRWRRLDYVKFSASFTVHSLALLAPFYFTWSALWVTFLFYTIGGL-GITVSYHRNLA 90

Query: 77  HKSFKLPNWLEYLFAYCGVQALEGDPIDWVSTHRYYHQVADTNKDPQRLEQRFLLLSNYY 136
           H+SFK+P WLEYL AYC + A++GDPIDWVSTHRY+HQ  D+ +DP   ++ F       
Sbjct: 91  HRSFKVPKWLEYLLAYCALLAIQGDPIDWVSTHRYHHQFTDSERDPHSPKEGF------- 150

Query: 137 GSNKRVNSRYFAIFKEIEKNVFLMVRKKVRRDNVEDLEKQSFYKFIHKTYIIHPIALAIL 196
                    +F+    I  + +L V K  RR NVEDL++Q FY+F+ KT + H + L   
Sbjct: 151 ---------WFSHLLWIYDSAYL-VSKCGRRANVEDLKRQWFYRFLQKTVLFHILGLGFF 210

Query: 197 LYAVGGMPFLIWGMVK------------------LGKQQWKTRDLSLNNGWMSLVSFGES 256
           L+ +GGM F+ WGM                     G + WKT D S N  W+S+ SFGES
Sbjct: 211 LFYLGGMSFVTWGMGVGAALEVHVTCLINSLCHIWGTRTWKTNDTSRNVWWLSVFSFGES 270

Query: 257 WHNNHHAFPYSARHGFEWWQIDFSWYAINFLQAIGVATNVKLP 282
           WHNNHHAF  SAR G EWWQID SWY + F + IG+AT+VK+P
Sbjct: 271 WHNNHHAFESSARQGLEWWQIDISWYIVRFFEIIGLATDVKVP 295

BLAST of IVF0025894 vs. TAIR 10
Match: AT3G15870.1 (Fatty acid desaturase family protein )

HSP 1 Score: 245.7 bits (626), Expect = 4.5e-65
Identity = 128/290 (44.14%), Postives = 168/290 (57.93%), Query Frame = 0

Query: 10  IERSPTSFGKEEWAEIHKGMAFIIFLLHLLSIFAPFQFSWSALWVAVALHVITGLFGMNL 69
           + R+  SF +  W         I    HLLS+ APF FSW A WV   L  I G+  + L
Sbjct: 79  VVRTKRSFWERSWTSWDVSKLVIFVGTHLLSLLAPFYFSWEAFWVFPWLVFINGIC-ITL 138

Query: 70  SYHRNLSHKSFKLPNWLEYLFAYCGVQALEGDPIDWVSTHRYYHQVADTNKDPQRLEQRF 129
           SYHRNLSH+SF LP WLEYLFAY GV A +GDPI+WVS HRY+H+  +T +DP    Q F
Sbjct: 139 SYHRNLSHRSFDLPKWLEYLFAYGGVLAFQGDPIEWVSNHRYHHKHCETQRDPHSPTQGF 198

Query: 130 LLLSNYYGSNKRVNSRYFAIFKEIEKNVFLMVRKKVRRDNVEDLEKQSFYKFIHKTYIIH 189
                         S    IF     +   ++      +NV+DL +Q FY+F+ +T ++H
Sbjct: 199 WF------------SHMAWIF-----DTSSILENCGGEENVDDLVRQPFYRFLQRTVLLH 258

Query: 190 PIALAILLYAVGGMPFLIWGM-----VKL-------------GKQQWKTRDLSLNNGWMS 249
            +A + L Y  GGMP L+WG+     V+L             G + W T D S NN W++
Sbjct: 259 MMAYSFLFYFCGGMPLLVWGIGITIAVRLHLTFLVNSVCHIWGTRAWNTSDFSKNNWWVA 318

Query: 250 LVSFGESWHNNHHAFPYSARHGFEWWQIDFSWYAINFLQAIGVATNVKLP 282
           +++ GE WHNNHHAF +SARHG EWWQ+D +W  I FL+AIG+ATNVKLP
Sbjct: 319 ILTLGEGWHNNHHAFEFSARHGLEWWQLDITWCLIRFLEAIGLATNVKLP 350

BLAST of IVF0025894 vs. TAIR 10
Match: AT1G06100.1 (Fatty acid desaturase family protein )

HSP 1 Score: 234.2 bits (596), Expect = 1.3e-61
Identity = 121/298 (40.60%), Postives = 172/298 (57.72%), Query Frame = 0

Query: 2   DVSMEPSEIERSPTSFGKEEWAEIHKGMAFIIFLLHLLSIFAPFQFSWSALWVAVALHVI 61
           D S +   + +   ++   +W +   G A  +  +HLL + APF + W A    + L ++
Sbjct: 8   DGSSQKKSVRKEKRAYVLRKWTQFDVGRASTVGTVHLLCLLAPFNYKWEAFRFGIILAIL 67

Query: 62  TGLFGMNLSYHRNLSHKSFKLPNWLEYLFAYCGVQALEGDPIDWVSTHRYYHQVADTNKD 121
           T L  +  SYHRNL+H+SFKLP WLEY FAY  + AL+GDP+DWVS HR++HQ  D+++D
Sbjct: 68  TNLC-ITFSYHRNLTHRSFKLPKWLEYPFAYSALLALQGDPLDWVSIHRFHHQFTDSDRD 127

Query: 122 PQRLEQRFLLLSNYYGSNKRVNSRYFAIFKEIEKNVFLMVRKKVRRDNVEDLEKQSFYKF 181
           P    + F              S    IF     +   +  K  RR+NV DL++Q FY+F
Sbjct: 128 PHSPIEGFWF------------SHVLWIF-----DTDYIREKCGRRNNVMDLKQQWFYRF 187

Query: 182 IHKTYIIHPIALAILLYAVGGMPFLIW-----GMVK-------------LGKQQWKTRDL 241
           + KT ++H +A   L+Y  GG+P+L W     G++               G Q W+T D 
Sbjct: 188 LKKTLVLHILAFWTLIYLWGGLPYLTWTVGFGGVIGYHGTWLVNSACHICGSQAWQTNDT 247

Query: 242 SLNNGWMSLVSFGESWHNNHHAFPYSARHGFEWWQIDFSWYAINFLQAIGVATNVKLP 282
           S N  W++L++ GESWHNNHHAF  SARHG EW+Q+D +WY I F QA+G+ATNVKLP
Sbjct: 248 SRNVWWLALLTMGESWHNNHHAFETSARHGLEWYQLDITWYLIWFFQALGLATNVKLP 287

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q949X01.8e-7950.00Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic OS=Arab... [more]
O657974.2e-6844.11Delta-9 acyl-lipid desaturase 1 OS=Arabidopsis thaliana OX=3702 GN=ADS1 PE=2 SV=... [more]
Q9FV683.0e-6644.33Icosanoyl-CoA 5-desaturase (Fragment) OS=Limnanthes douglasii OX=28973 PE=1 SV=1[more]
Q9SID23.9e-6645.23Delta-9 acyl-lipid desaturase 2 OS=Arabidopsis thaliana OX=3702 GN=ADS2 PE=2 SV=... [more]
P0DOW39.7e-6545.08Acyl-CoA 5-desaturase AL21 OS=Anemone leveillei OX=212809 GN=AL21 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A5D3DZT86.0e-118100.00Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase OS=Cucumis melo var. m... [more]
A0A6J1JGI09.0e-11466.89palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS... [more]
A0A6J1H9D07.9e-11067.94palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS... [more]
A0A6J1DHZ74.5e-9758.33palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS... [more]
A0A6J1DJP01.0e-9655.23palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS... [more]
Match NameE-valueIdentityDescription
XP_038879870.11.01e-16575.00palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [B... [more]
TYK28760.12.49e-151100.00palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase [Cucumis melo var. mak... [more]
XP_022988176.19.60e-14466.89palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [C... [more]
XP_023533044.13.38e-13966.00acyl-CoA C20 Delta5-desaturase-like [Cucurbita pepo subsp. pepo][more]
XP_022961086.17.84e-13967.94palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [C... [more]
Match NameE-valueIdentityDescription
AT3G15850.11.3e-8050.00fatty acid desaturase 5 [more]
AT1G06080.13.0e-6944.11delta 9 desaturase 1 [more]
AT2G31360.12.8e-6745.2316:0delta9 desaturase 2 [more]
AT3G15870.14.5e-6544.14Fatty acid desaturase family protein [more]
AT1G06100.11.3e-6140.60Fatty acid desaturase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR015876Acyl-CoA desaturasePRINTSPR00075FACDDSATRASEcoord: 72..92
score: 32.33
coord: 109..138
score: 24.56
coord: 236..250
score: 60.35
IPR015876Acyl-CoA desaturasePANTHERPTHR11351ACYL-COA DESATURASEcoord: 19..281
IPR015876Acyl-CoA desaturaseCDDcd03505Delta9-FADS-likecoord: 49..279
e-value: 1.15591E-46
score: 153.095
NoneNo IPR availablePANTHERPTHR11351:SF94BNAC05G37460D PROTEINcoord: 19..281

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
IVF0025894.1IVF0025894.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006633 fatty acid biosynthetic process
cellular_component GO:0005789 endoplasmic reticulum membrane
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0009979 16:0 monogalactosyldiacylglycerol desaturase activity
molecular_function GO:0016717 oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water