IVF0025767 (gene) Melon (IVF77) v1

Overview
NameIVF0025767
Typegene
OrganismCucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
DescriptiontRNA (guanine(37)-N1)-methyltransferase
Locationchr05: 25844943 .. 25848953 (-)
RNA-Seq ExpressionIVF0025767
SyntenyIVF0025767
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTGGACGAAAGCAAATTCGACGTGCATCTGAATCTCTGGGCATTAAGAATTCCGAGGGAACTTTGTAAGGCCGCCATTAAAATACTGAACGGGTACGCCATGTTTTGTGTTATATTGTTTCTTTGTTATACTTTGCCAATTATTCGATTTCAATTAATCATCTGGGTAATGGCATATAGTATTAGTGTTACTTATATTTTGTTCTCGTTGAACTGTTCATCTTTCTCATCCTAGTTCATCATTATTCGAAAACTTATCAGAATTTTCCTGATAGATTTTAGTTTTTTTTCTTTGAAAGTTCTTGTTGTTAATTTTTGTTTCAAAGCAGACTTTTCTTTGGCCCTCAGTTCGTTTCTGTTGAAAATTTTCAGGTACTTAATTGACAAGCCCCGGATTAAACCTGTGACTGAAGATCCAACATGTGATAAGAACCGTTATGTTATATTATCTGAAAAGGTTCAAACTCCAGGTAAAAATCTCCATCTCCGTCCTTATTTTTTTCCTGTTAACTATATGCTTAGATTGCAATTTCACGTTCTTTATAGAAACTCTTCTGTAGGACATTCTGTTCGCTGAAGTTCCAATTCTTGTTCATTGATTTTTTTTCATGTTATCTATTCATGCACTCAAGTTCTTAAATAGTACTAAAATTATCGAGCGAGTAATTTTTTATCTTAAAACATTAATTTCTGCAGGGTATAACCATTAACTTGTATGCATCTTAAGTTTAGAAAATTGTTCGAAAACAGCCTATATAACACATGAAATCCAGCTTTAAAAAATCTAAACATCGATAAAAGAAACCAAATTAACGTTGTGTAACTAGGTAATTTTCATAAAAATAGTCTTGTTTCCCTTTAGAAATATTAACCACCTTTTCTCTTCTTTTGCTCTTATTTCTCGATGGAATTTTCCTCAATCATGTCCTTAGATGACACCATTTTTCTATCTTGTGATTCTTTGAATGGTCAGAATTATCAGAAATTCCAGAAATGAAGCTTGAAGAATTGAAAGGCTTATGCATGTTTGAACGAGTTCCTTATTCACTAACCCTTGGATATTCATATTGGGGAGCAGGTATTTTCCATAATTCTACCCTTTATCGCACATCGTGCAAAAATTTTACTTTCACCTTAGGATTGCCTGTTGAACTCCAGACATTTATAGTTCCCTTTAAGTTTCTAATTGCAAGTCTGAAGTGTTATGTAAGGCATATCTAGCACCATTTTTAATGATCCCATTCCCATTACTCAAAATCCAAACGTTTTCTGCATTGTGACTGTACACGTGCTGATAGAATCTGGTTGTTTTTGCCTCTCATTTGATATTATAACAACTACATGTGATGTGACCGGTCTACATCAAGTGGTTGCAAGTATAAGAACAATGTTCGATTTCTGAAAGATAGCTTAAACAATTCTGTATGCAAATTTACATGTCATTTGATGTGTTTGTATTGTACATGCTCGACGAACTTACTGTGTGGTTGCTCTGTAGATCATATTCTCAAGAAGATTTTGCCTCCTGAAGTGGAAGTGCCTTCATCTTTTGAGACAATAGTTAAGTACTCTAACCCCTGCCGTCTTCCACCTACGTTTTCCTTTCTATAGTTTGTCTCCATTTCCATATGCTCTATATAGCTTTTATGTGCGTTCTTTTGTTGCTCTGTATTGCATTTTGGTACCACTTGTTTATGTGACATTGGTTGTAACATTGCTTTTTCACGTACAACTATTGACAGTGAGATAATTGTGTACTGTCCTTTGCAGGTCACGTTGCTCACCTGAATATACACGACGAATTGCTTCCATACAAGGATGTCATTGCGAAGGTTATTTATGATGTATGTCATCAGTATCTATTTACTCATTTTCCTAATTATTTTGAAATGTACATTGTGATTTATTGATCACTATAATTTAGCTGTTACTTCATTTTTTTATTTTCCTTCGTGCATATGTTGGGGGTACCTTTTTAAGAAATTCAAATGTTCAGAATTGGATATTTAATATTTTTATCCTCCCCCTCCATCTAGCAACACACACACACACACACACACATTTTCCCTATTGAAACTGTTTCTGTAACTATTTCTTTTTGTAATCTTCGCAGAAAAATTATCCGAGGATTAAAACAGTTGTAAATAAAGTGGGTTCTATAACAAATGAGTTTCGGGTTCCAAAGTTTGAAATACTGAAGGGAGAGAATGATATGGTAACAGAAGTGAAGCAATATGGAGCGACATTTAAGCTTGACTACAGTTTGGTCTACTGGAACTCAAGATTGGAACATGAACACATAAGGCTGGTTTCACTCTTCCAACCAGGAGAGGTAATTTGTGACATGTTTGCTGGTATTGGTCCTTTTGCAATTCCAGCCGCCCAGAAAGAATGTATAGTATATGCCAATGACTTGAATCCAGACAGCGTCCGATATCTGAAGGTTAATGCAGAAATCAATAAAGTTAGTGGTCGCGTTCATGTGTACAATTTGGATGCTAGGAAATTTATTTCTCAATTGATGATAGTTCCCCCTAATGAAAGTAATTCAGAAACTGTCACCTCAATACTAAAAGCTTCTGAGGAAGGCATTGGAGGGACCAATCAAGAATCACCGTTGGCAAATGGTATGCTGACTGGTATTATATTATTATCATTTAAAGAAATTTTTATATCGTCTTCAGGAGATTAGTGTAGATGATCTTAATTTTCTTTCTTGCTTCAGTTCAGGTGCAAGAAGTTCAAGATATTGAAATTAGCAATAAATTTGTAGAACATTCAAGGAGTGCTGATATATCCGTTGCTGTTCTTAAAAGATCCTCGGAAAGTTGTGAGAATGAGAAAGGTAACATACTGTACCATATCTTGTGATTGAACAACCAAGACCATTTCTTCCTATAATTTTCTTTGCAGATTTTTTTTTCTTTTGATGAAAATTTGCTTTGCAGAAGACAGAATTGCTGATGATGCTTCCACCCGTGTAGCCGGTAGGAGAAATGGAAACGGAAATAAGAGGATGAAGGGGTCCACCAATGTATCTCATGCTAGGACCTGGGAACACATCGATCATGTGATTATGAACCTCCCTGCTTCTGCTTTACATTTTCTTGGTACGAATGGACTTTTTTCTTTTTAATTTCATTTCTCTTATTGCAGTTGTTTTAGCATATTTCTCTACATTTGTGTGTCGGAATGAAGAGTTGAAAGTTGGGGAAATAACAATGTTAGGAAAGGGAAACAATTACTTTTAAATGCTTCAGATATGAAGGGAAACCATAAACGATTCTTTTTCTCTTACTATCACCATGACAGACCATTTTGGATATGTCATATTTACTAATTCCTACCTCTCAAATGGAGTTTTGTTGTAAGTTTCGTAGTCACTCTCTATACCTATCTTACGAGTTTCTTCAATCTCCTTTAGCTCTTGGGTGATGGAGAGTGCCTTATCTCCTCCGCTCAATTATCTCAACTCGTCTTTTGTATCCTTATCTATATTTTGCTTAGTTAACCAATGAAGTATATTTTGTTATTTTCTTGCCAGATGCATTTAGAGGCTCAATTAAGAAGAAATACTGGAAGGGCTCCCTTCCTTGGATTCACTGTTACTGCTTCATGCGAGCAAATGAAAATCAAGATTTTATCATTTCGGTATGTTTATACTAATTACTATTGATCCTTATTTGTCTCTGTCTTCTTTTGATAATGTAATTTTCTCACCATTCTATTTGGATATTTGTCTCGAAAAAAGTCGTCTTTCTTATCATACCGTCCTCTTTTAACCGTTCCACTCCAGGTGGCTGAGTCTGCCTTGAATGCTAATATTCAAGACCCCATTTTTCATAGGGTCAGAGATGTTGCACCAAACAAGGTATTGCTCTGTGTGTGTGTATAAATGGCAACTATTTGGTTTTTAACTCATTTTTCTTTTTTGTTTTTTTATCAGGCTATGTACTGTTTGAGCTTTAGGCTTCCGGAACAATGTGTCAATGAGGACACGACTAGCTAA

mRNA sequence

ATGTTGGACGAAAGCAAATTCGACGTGCATCTGAATCTCTGGGCATTAAGAATTCCGAGGGAACTTTGTAAGGCCGCCATTAAAATACTGAACGGGTACTTAATTGACAAGCCCCGGATTAAACCTGTGACTGAAGATCCAACATGTGATAAGAACCGTTATGTTATATTATCTGAAAAGGTTCAAACTCCAGAATTATCAGAAATTCCAGAAATGAAGCTTGAAGAATTGAAAGGCTTATGCATGTTTGAACGAGTTCCTTATTCACTAACCCTTGGATATTCATATTGGGGAGCAGGTCACGTTGCTCACCTGAATATACACGACGAATTGCTTCCATACAAGGATGTCATTGCGAAGGTTATTTATGATAAAAATTATCCGAGGATTAAAACAGTTGTAAATAAAGTGGGTTCTATAACAAATGAGTTTCGGGTTCCAAAGTTTGAAATACTGAAGGGAGAGAATGATATGGTAACAGAAGTGAAGCAATATGGAGCGACATTTAAGCTTGACTACAGTTTGGTCTACTGGAACTCAAGATTGGAACATGAACACATAAGGCTGGTTTCACTCTTCCAACCAGGAGAGGTAATTTGTGACATGTTTGCTGGTATTGGTCCTTTTGCAATTCCAGCCGCCCAGAAAGAATGTATAGTATATGCCAATGACTTGAATCCAGACAGCGTCCGATATCTGAAGGTTAATGCAGAAATCAATAAAGTTAGTGGTCGCGTTCATGTGTACAATTTGGATGCTAGGAAATTTATTTCTCAATTGATGATAGTTCCCCCTAATGAAAGTAATTCAGAAACTGTCACCTCAATACTAAAAGCTTCTGAGGAAGGCATTGGAGGGACCAATCAAGAATCACCGTTGGCAAATGTTCAGGTGCAAGAAGTTCAAGATATTGAAATTAGCAATAAATTTGTAGAACATTCAAGGAGTGCTGATATATCCGTTGCTGTTCTTAAAAGATCCTCGGAAAGTTGTGAGAATGAGAAAGAAGACAGAATTGCTGATGATGCTTCCACCCGTGTAGCCGGTAGGAGAAATGGAAACGGAAATAAGAGGATGAAGGGGTCCACCAATGTATCTCATGCTAGGACCTGGGAACACATCGATCATGTGATTATGAACCTCCCTGCTTCTGCTTTACATTTTCTTGATGCATTTAGAGGCTCAATTAAGAAGAAATACTGGAAGGGCTCCCTTCCTTGGATTCACTGTTACTGCTTCATGCGAGCAAATGAAAATCAAGATTTTATCATTTCGGTGGCTGAGTCTGCCTTGAATGCTAATATTCAAGACCCCATTTTTCATAGGGTCAGAGATGTTGCACCAAACAAGGCTATGTACTGTTTGAGCTTTAGGCTTCCGGAACAATGTGTCAATGAGGACACGACTAGCTAA

Coding sequence (CDS)

ATGTTGGACGAAAGCAAATTCGACGTGCATCTGAATCTCTGGGCATTAAGAATTCCGAGGGAACTTTGTAAGGCCGCCATTAAAATACTGAACGGGTACTTAATTGACAAGCCCCGGATTAAACCTGTGACTGAAGATCCAACATGTGATAAGAACCGTTATGTTATATTATCTGAAAAGGTTCAAACTCCAGAATTATCAGAAATTCCAGAAATGAAGCTTGAAGAATTGAAAGGCTTATGCATGTTTGAACGAGTTCCTTATTCACTAACCCTTGGATATTCATATTGGGGAGCAGGTCACGTTGCTCACCTGAATATACACGACGAATTGCTTCCATACAAGGATGTCATTGCGAAGGTTATTTATGATAAAAATTATCCGAGGATTAAAACAGTTGTAAATAAAGTGGGTTCTATAACAAATGAGTTTCGGGTTCCAAAGTTTGAAATACTGAAGGGAGAGAATGATATGGTAACAGAAGTGAAGCAATATGGAGCGACATTTAAGCTTGACTACAGTTTGGTCTACTGGAACTCAAGATTGGAACATGAACACATAAGGCTGGTTTCACTCTTCCAACCAGGAGAGGTAATTTGTGACATGTTTGCTGGTATTGGTCCTTTTGCAATTCCAGCCGCCCAGAAAGAATGTATAGTATATGCCAATGACTTGAATCCAGACAGCGTCCGATATCTGAAGGTTAATGCAGAAATCAATAAAGTTAGTGGTCGCGTTCATGTGTACAATTTGGATGCTAGGAAATTTATTTCTCAATTGATGATAGTTCCCCCTAATGAAAGTAATTCAGAAACTGTCACCTCAATACTAAAAGCTTCTGAGGAAGGCATTGGAGGGACCAATCAAGAATCACCGTTGGCAAATGTTCAGGTGCAAGAAGTTCAAGATATTGAAATTAGCAATAAATTTGTAGAACATTCAAGGAGTGCTGATATATCCGTTGCTGTTCTTAAAAGATCCTCGGAAAGTTGTGAGAATGAGAAAGAAGACAGAATTGCTGATGATGCTTCCACCCGTGTAGCCGGTAGGAGAAATGGAAACGGAAATAAGAGGATGAAGGGGTCCACCAATGTATCTCATGCTAGGACCTGGGAACACATCGATCATGTGATTATGAACCTCCCTGCTTCTGCTTTACATTTTCTTGATGCATTTAGAGGCTCAATTAAGAAGAAATACTGGAAGGGCTCCCTTCCTTGGATTCACTGTTACTGCTTCATGCGAGCAAATGAAAATCAAGATTTTATCATTTCGGTGGCTGAGTCTGCCTTGAATGCTAATATTCAAGACCCCATTTTTCATAGGGTCAGAGATGTTGCACCAAACAAGGCTATGTACTGTTTGAGCTTTAGGCTTCCGGAACAATGTGTCAATGAGGACACGACTAGCTAA

Protein sequence

MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEKVQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGAGHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKECIVYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSETVTSILKASEEGIGGTNQESPLANVQVQEVQDIEISNKFVEHSRSADISVAVLKRSSESCENEKEDRIADDASTRVAGRRNGNGNKRMKGSTNVSHARTWEHIDHVIMNLPASALHFLDAFRGSIKKKYWKGSLPWIHCYCFMRANENQDFIISVAESALNANIQDPIFHRVRDVAPNKAMYCLSFRLPEQCVNEDTTS
Homology
BLAST of IVF0025767 vs. ExPASy Swiss-Prot
Match: F6HH45 (tRNA (guanine(37)-N1)-methyltransferase 2 OS=Vitis vinifera OX=29760 GN=VIT_11s0016g04350 PE=3 SV=1)

HSP 1 Score: 629.4 bits (1622), Expect = 3.3e-179
Identity = 322/501 (64.27%), Postives = 383/501 (76.45%), Query Frame = 0

Query: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60
           +LDESKFD+ L LWALRIP +LCK A +ILNGYL+DKPR+KP+TEDPTC+ NRY+ILSE+
Sbjct: 2   LLDESKFDLQLKLWALRIPSQLCKVASRILNGYLLDKPRVKPITEDPTCETNRYIILSER 61

Query: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGAGHV------------------ 120
           ++ P+LS+IPE K +ELK LC  E VPYSLTLGYSYW A HV                  
Sbjct: 62  IKNPDLSDIPEDKRDELKVLCKIEVVPYSLTLGYSYWSADHVLKQILPDGVEVPSSFETI 121

Query: 121 -------AHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGE 180
                  AHLNI  ELLPYKDVIAKVIYDKNYPRIKTVVNKVG+ITNEFRVPKFEIL G+
Sbjct: 122 TFSLCHIAHLNITGELLPYKDVIAKVIYDKNYPRIKTVVNKVGTITNEFRVPKFEILVGK 181

Query: 181 NDMVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQ 240
           +DMVTEVKQY ATFKLDYSLVYWNSRLEHEH+RLVS F+PG++ICDMF+G+GPFAIPAAQ
Sbjct: 182 DDMVTEVKQYRATFKLDYSLVYWNSRLEHEHMRLVSQFRPGQIICDMFSGVGPFAIPAAQ 241

Query: 241 KECIVYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSETVTS 300
           K C+VYANDLNPDS+RYLK+NA+INKV   +  YN+DARKFISQLM VP +E   E    
Sbjct: 242 KGCLVYANDLNPDSIRYLKINAKINKVDDNIWAYNMDARKFISQLMEVPVHEILPEPDVP 301

Query: 301 ILKASEEGIGGTNQESPLAN----VQVQEVQDIEISN-KFVEHSRS-ADISVAVLKRSSE 360
           +LKA+EE     N ++   N    V+   V   + SN   +E S + AD +VA +K+ S 
Sbjct: 302 VLKATEECSIQANVQTESQNGGLTVEAIGVHSDDSSNMDGIEGSCTVADDTVAAVKKHSG 361

Query: 361 SCENEKEDRIADDASTRVAGRRNGNGNKRMKGSTNVSHARTWEHIDHVIMNLPASALHFL 420
           SC  E+E+   D+A+  V GRR    NKRM+GS + S+ + WEH+DHVIMNLPASA+ FL
Sbjct: 362 SC--EEENGTYDNATVSVVGRRKDRMNKRMRGSESSSNIKPWEHVDHVIMNLPASAIQFL 421

Query: 421 DAFRGSIKKKYWKGSLPWIHCYCFMRANENQDFIISVAESALNANIQDPIFHRVRDVAPN 470
           DAFRG I++KYWKGSLPWIHCYCF+RANE  + IIS AE+ALNA+IQ+PI HRVRDVAPN
Sbjct: 422 DAFRGLIQRKYWKGSLPWIHCYCFIRANETNEMIISEAETALNASIQEPILHRVRDVAPN 481

BLAST of IVF0025767 vs. ExPASy Swiss-Prot
Match: Q93YU6 (tRNA (guanine(37)-N1)-methyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=At3g56120 PE=2 SV=1)

HSP 1 Score: 568.9 bits (1465), Expect = 5.2e-161
Identity = 292/490 (59.59%), Postives = 351/490 (71.63%), Query Frame = 0

Query: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60
           M DESKFDV+L LWALRIPRELCK+A +ILNGY+++ PRIKP+TEDPTC+K R VILSE 
Sbjct: 1   MFDESKFDVNLKLWALRIPRELCKSASRILNGYMLNMPRIKPITEDPTCEKTRLVILSES 60

Query: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGA--------------------- 120
           V+  +LSEIPE KL +LK L   E VP+S+TLGYSYW A                     
Sbjct: 61  VKNADLSEIPEEKLNQLKKLSELEVVPHSVTLGYSYWSADHLLKQILPDGLDIPSSFETI 120

Query: 121 GHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMV 180
           GH+AHLN+HDELLP+KDVIAKVIYDKNYPRIKT+VNKVG+I+NEFRVPKFE+L GEN M 
Sbjct: 121 GHIAHLNLHDELLPFKDVIAKVIYDKNYPRIKTIVNKVGTISNEFRVPKFEVLAGENGME 180

Query: 181 TEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKECI 240
           TEVKQYGA FKLDY LVYWNSRLEHEH+RL SLF+PGE +CDMFAGIGPFAIPAAQK C 
Sbjct: 181 TEVKQYGARFKLDYGLVYWNSRLEHEHMRLSSLFKPGETVCDMFAGIGPFAIPAAQKGCF 240

Query: 241 VYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSETVTSILKA 300
           VYANDLNPDSVRYLK+NA+ NKV   + V+N+DARKF S LM V   E N ++V    K 
Sbjct: 241 VYANDLNPDSVRYLKINAKFNKVDDLICVHNMDARKFFSHLMAVSTCEDNLQSVADNDKT 300

Query: 301 SEEGI--GGTNQESPLANVQVQEVQDIEISNKFVEHSRSADISVAVLKRSSESCENEKED 360
            E  +  GG    S          ++I  SN        A I+  +      S   + E+
Sbjct: 301 KEAAVSRGGETNSSG---------EEIRESN--------ASINEPLGANKKPSGTTKTEN 360

Query: 361 RIADDASTRVAGRRNGNGNKRMKGSTNVSHARTWEHIDHVIMNLPASALHFLDAFRGSIK 420
            +  D  +       G+ NKR++  T +  A+ WEHIDHVIMNLPASAL FLD+F   I+
Sbjct: 361 GVGKDCKS-----IEGHANKRLR-QTLLPIAKPWEHIDHVIMNLPASALQFLDSFSNVIQ 420

Query: 421 KKYWKGSLPWIHCYCFMRANENQDFIISVAESALNANIQDPIFHRVRDVAPNKAMYCLSF 468
           KKYWKG LP IHCYCF+RA+E  +FII+ AE+AL  +I+DP+FH+VRDVAPNKAM+CLSF
Sbjct: 421 KKYWKGPLPLIHCYCFIRASETTEFIIAEAETALKFHIEDPVFHKVRDVAPNKAMFCLSF 467

BLAST of IVF0025767 vs. ExPASy Swiss-Prot
Match: C5XX79 (tRNA (guanine(37)-N1)-methyltransferase OS=Sorghum bicolor OX=4558 GN=Sb04g025390 PE=3 SV=1)

HSP 1 Score: 565.1 bits (1455), Expect = 7.5e-160
Identity = 289/468 (61.75%), Postives = 355/468 (75.85%), Query Frame = 0

Query: 2   LDESKFDVHLNLWALRIPRELCKAAIKIL-NGYLIDKPRIKPVTEDPTCDKNRYVILSEK 61
           LDESKF+  L LWALRIPREL  A  ++L +GYL+DKPR+K V EDP  DKNR VILSEK
Sbjct: 4   LDESKFEQRLQLWALRIPRELSSAVTRLLRSGYLLDKPRVKTVVEDPEGDKNRLVILSEK 63

Query: 62  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGAGHVAHLNIHDELLPYKDVIAK 121
           +Q P+LS++P+ +L+ LK LC  + VPY+LTLGYSYW AGHVAHLNI D+LLPYK+VIAK
Sbjct: 64  IQKPDLSDMPQQELDSLKQLCNVDVVPYTLTLGYSYWSAGHVAHLNISDDLLPYKNVIAK 123

Query: 122 VIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMVTEVKQYGATFKLDYSLVYWNS 181
           VIYDKNYPRI+TV NKVG+ITNEFRVPKFEIL G+NDMVTEVKQYGATFKLDY LVYWNS
Sbjct: 124 VIYDKNYPRIQTVANKVGTITNEFRVPKFEILAGKNDMVTEVKQYGATFKLDYGLVYWNS 183

Query: 182 RLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKECIVYANDLNPDSVRYLKVNAEIN 241
           RL+HEHIRLVSLF+ G+VICDMFAGIGPFAIPAAQK C+VYANDLNPDSV YL+ NA+IN
Sbjct: 184 RLDHEHIRLVSLFKKGDVICDMFAGIGPFAIPAAQKGCVVYANDLNPDSVHYLRTNAKIN 243

Query: 242 KVSGRVHVYNLDARKFISQLMIVPPNESNSE-TVTSILKASEEGIGGTNQESPLANVQVQ 301
           KV   +  YN+DAR F+  LM VP  E+ S+  V +     +EG+      S  +N    
Sbjct: 244 KVDDYIFAYNMDARVFMQNLMTVPGLETGSDCQVAADESYPKEGVPANENSS--SNGNHN 303

Query: 302 EVQDIEISNKFVEHSRSADISVAVLKRSSESCENEKEDRIADDASTRVAGRRNGNGNKRM 361
           +V+  E S      S  A  +    +++SE CE++ +D   D + T+   RRN   NKR+
Sbjct: 304 DVR--EGSQNGANESSVASTTAKKRQQTSEECESDCQD--GDASQTK---RRN---NKRV 363

Query: 362 KGSTNVSHARTWEHIDHVIMNLPASALHFLDAFRGSIKKKYWKGSLPWIHCYCFMRANEN 421
           +G      ++ WEH DHV+MNLPASAL FLD F G ++KKYW GSLPWIHCYCF+R++E+
Sbjct: 364 RG-PGPPPSKPWEHFDHVLMNLPASALQFLDCFDGLVQKKYWTGSLPWIHCYCFIRSSES 423

Query: 422 QDFIISVAESALNANIQDPIFHRVRDVAPNKAMYCLSFRLPEQCVNED 468
           ++ I+S   + LNA I +PIFHRVRDVAPNKAM+CLSF+LP +C+ ED
Sbjct: 424 EESILS---NKLNAKIAEPIFHRVRDVAPNKAMFCLSFKLPMECLRED 455

BLAST of IVF0025767 vs. ExPASy Swiss-Prot
Match: A9T6G5 (tRNA (guanine(37)-N1)-methyltransferase OS=Physcomitrium patens OX=3218 GN=PHYPADRAFT_141055 PE=3 SV=1)

HSP 1 Score: 376.3 bits (965), Expect = 5.0e-103
Identity = 208/471 (44.16%), Postives = 279/471 (59.24%), Query Frame = 0

Query: 2   LDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEKV 61
           +D+S F V L   ALRIP++       +L+ YL+D PR+K + EDP     R ++ SE +
Sbjct: 11  IDKSAFVVRLPRVALRIPKQKSHRVASLLSKYLLDMPRVKHIVEDPQDPSMRLLLFSEAI 70

Query: 62  QTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGA---------------------G 121
             P+L  + E K+  +K +   + V + + L YSYW                       G
Sbjct: 71  AQPDLEGVSEEKILAVKEIVPLDVVQHEVVLDYSYWPVEHILKEILPAGCEVPSSFETIG 130

Query: 122 HVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMVT 181
           H+AHLN+ ++LL YK +IA+VI DKN P++KTVVNKVG+ITNEFRVP+FEIL GE  +VT
Sbjct: 131 HIAHLNLREDLLTYKKIIAEVILDKN-PKLKTVVNKVGTITNEFRVPEFEILAGEPSLVT 190

Query: 182 EVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKECIV 241
           E+KQ+GATF+LDY +VYWNSRLE EH RL + F+PG+VI DMFAGIGPFAIPAAQ+ C V
Sbjct: 191 EIKQHGATFRLDYGMVYWNSRLEGEHKRLFAQFKPGQVIVDMFAGIGPFAIPAAQQGCAV 250

Query: 242 YANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSETVTSILKAS 301
           YANDLNP SV++LK+N++INKV   +  +NLDAR+F+ +L  V   E     + S    +
Sbjct: 251 YANDLNPTSVKFLKLNSDINKVGESIKAFNLDAREFMRKL--VTEEEGCVGEIASSTLIA 310

Query: 302 EEGIGGTNQESPLANVQVQEVQDIEISNKFVEHSRSADISVAVLKRSSESCENEKEDRIA 361
           E  + GT   S                        S + S ++ + S      + E+R A
Sbjct: 311 ENSLNGTRIPS------------------------SKENSSSLTEGSDMPSSKKPEERKA 370

Query: 362 DDASTRVAGRRNGNGNKRMKGSTNVSHARTWEHIDHVIMNLPASALHFLDAFRGSIKKKY 421
             A              +MK    +S  + WEH DHV+MNLPASAL FLD   G + K  
Sbjct: 371 KKAKVSEV--------VKMK----ISDVKPWEHFDHVVMNLPASALEFLDVLNGLLCKGR 430

Query: 422 WKGSLPWIHCYCFMRANENQDFIISVAESALNANIQDPIFHRVRDVAPNKA 452
           WKG++P +HCYCFMR+NE    I+  AE+ L  +I DP  + VRDVAPNKA
Sbjct: 431 WKGTMPRVHCYCFMRSNETNADIVKKAETYLGGSIADPDVYVVRDVAPNKA 442

BLAST of IVF0025767 vs. ExPASy Swiss-Prot
Match: F4NUJ6 (tRNA (guanine(37)-N1)-methyltransferase OS=Batrachochytrium dendrobatidis (strain JAM81 / FGSC 10211) OX=684364 GN=TRM5 PE=3 SV=1)

HSP 1 Score: 251.5 bits (641), Expect = 1.8e-65
Identity = 158/486 (32.51%), Postives = 246/486 (50.62%), Query Frame = 0

Query: 2   LDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEKV 61
           L+ + F+    L AL+IP      A++ L  +++  PR++ + +DPT  K R ++L   +
Sbjct: 16  LNHALFESSHTLPALKIPAHASGIAMETLRSHILVAPRLRTIVDDPTDKKWRLLLLDPSM 75

Query: 62  QTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYW-----------------GA----G 121
           +  E++++P   L+E       + V +++ L Y YW                 GA    G
Sbjct: 76  EASEINDLPN-PLKEFALKHEAKLVKHTIELKYDYWTSDQVLRSILPDEMETPGAFETVG 135

Query: 122 HVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMVT 181
           H+AHLN+ D   P+K +I +VI DK+   IKTVVNK+ +I + FR  + EIL G NDM  
Sbjct: 136 HIAHLNLRDRYQPFKHIIGQVILDKS-SHIKTVVNKLDNIDHTFRFFQMEILAGINDMNA 195

Query: 182 EVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQ-KECI 241
           ++K+ G  F  D+S VYWNSRL+ EH R++ LF   ++ICD+FAG+GPFA+PAA+ K C+
Sbjct: 196 KLKEGGCFFHFDFSKVYWNSRLQGEHDRIIKLFGQNDLICDVFAGVGPFALPAAKHKRCV 255

Query: 242 VYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSETVTSILKA 301
           V+ANDLNP S +YL  N ++NK+  R+  +N+D R+FI Q                    
Sbjct: 256 VFANDLNPQSFKYLMENIKLNKLETRILPFNMDGRQFIKQ-------------------- 315

Query: 302 SEEGIGGTNQESPLANVQVQEVQDIEISNKFVEHSRSADISVAVLKRSSESCENEKEDRI 361
                             ++++ +  I NK  +   +++      K+ +   E+     +
Sbjct: 316 -----------------SLEDLNNPAIWNKITKQKPTSND-----KKRNRKVESPTVAPL 375

Query: 362 ADDASTRVAGRRNGNGNKRMKGSTNVSHARTWEHIDHVIMNLPASALHFLDAFRG---SI 421
            D  +  ++G R                     H  H +MNLPA+A+ FLDAF G    +
Sbjct: 376 TDQPA--ISGIR---------------------HFKHYVMNLPATAIEFLDAFHGLYSGM 434

Query: 422 KKKYWKGSLPWIHCYCFMRANENQDFIISVAESALNANIQDPIF--HRVRDVAPNKAMYC 461
           +       LP IHC+CF  A + +  +I   E  +   +   +   H VR VAPNK M C
Sbjct: 436 RDVIMDSDLPTIHCHCFSNAKDVKADVIERVERVIGMPLGSNLIMVHSVRTVAPNKDMLC 434

BLAST of IVF0025767 vs. ExPASy TrEMBL
Match: A0A5D3CFY1 (tRNA (guanine(37)-N1)-methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold332G00100 PE=3 SV=1)

HSP 1 Score: 953.4 bits (2463), Expect = 3.6e-274
Identity = 470/470 (100.00%), Postives = 470/470 (100.00%), Query Frame = 0

Query: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60
           MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK
Sbjct: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60

Query: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGAGHVAHLNIHDELLPYKDVIAK 120
           VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGAGHVAHLNIHDELLPYKDVIAK
Sbjct: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGAGHVAHLNIHDELLPYKDVIAK 120

Query: 121 VIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMVTEVKQYGATFKLDYSLVYWNS 180
           VIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMVTEVKQYGATFKLDYSLVYWNS
Sbjct: 121 VIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMVTEVKQYGATFKLDYSLVYWNS 180

Query: 181 RLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKECIVYANDLNPDSVRYLKVNAEIN 240
           RLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKECIVYANDLNPDSVRYLKVNAEIN
Sbjct: 181 RLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKECIVYANDLNPDSVRYLKVNAEIN 240

Query: 241 KVSGRVHVYNLDARKFISQLMIVPPNESNSETVTSILKASEEGIGGTNQESPLANVQVQE 300
           KVSGRVHVYNLDARKFISQLMIVPPNESNSETVTSILKASEEGIGGTNQESPLANVQVQE
Sbjct: 241 KVSGRVHVYNLDARKFISQLMIVPPNESNSETVTSILKASEEGIGGTNQESPLANVQVQE 300

Query: 301 VQDIEISNKFVEHSRSADISVAVLKRSSESCENEKEDRIADDASTRVAGRRNGNGNKRMK 360
           VQDIEISNKFVEHSRSADISVAVLKRSSESCENEKEDRIADDASTRVAGRRNGNGNKRMK
Sbjct: 301 VQDIEISNKFVEHSRSADISVAVLKRSSESCENEKEDRIADDASTRVAGRRNGNGNKRMK 360

Query: 361 GSTNVSHARTWEHIDHVIMNLPASALHFLDAFRGSIKKKYWKGSLPWIHCYCFMRANENQ 420
           GSTNVSHARTWEHIDHVIMNLPASALHFLDAFRGSIKKKYWKGSLPWIHCYCFMRANENQ
Sbjct: 361 GSTNVSHARTWEHIDHVIMNLPASALHFLDAFRGSIKKKYWKGSLPWIHCYCFMRANENQ 420

Query: 421 DFIISVAESALNANIQDPIFHRVRDVAPNKAMYCLSFRLPEQCVNEDTTS 471
           DFIISVAESALNANIQDPIFHRVRDVAPNKAMYCLSFRLPEQCVNEDTTS
Sbjct: 421 DFIISVAESALNANIQDPIFHRVRDVAPNKAMYCLSFRLPEQCVNEDTTS 470

BLAST of IVF0025767 vs. ExPASy TrEMBL
Match: A0A1S3C7L3 (tRNA (guanine(37)-N1)-methyltransferase OS=Cucumis melo OX=3656 GN=LOC103497610 PE=3 SV=1)

HSP 1 Score: 938.3 bits (2424), Expect = 1.2e-269
Identity = 468/491 (95.32%), Postives = 470/491 (95.72%), Query Frame = 0

Query: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60
           MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK
Sbjct: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60

Query: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGA--------------------- 120
           VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGA                     
Sbjct: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120

Query: 121 GHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMV 180
           GHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMV
Sbjct: 121 GHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMV 180

Query: 181 TEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKECI 240
           TEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKECI
Sbjct: 181 TEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKECI 240

Query: 241 VYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSETVTSILKA 300
           VYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFIS+LMIVPPNESNSETVTSILKA
Sbjct: 241 VYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFISRLMIVPPNESNSETVTSILKA 300

Query: 301 SEEGIGGTNQESPLANVQVQEVQDIEISNKFVEHSRSADISVAVLKRSSESCENEKEDRI 360
           SEEGIGGTNQESPLANVQVQEVQDIEISNKFVEHSRSADISVAVLKRSSESCENEK+DRI
Sbjct: 301 SEEGIGGTNQESPLANVQVQEVQDIEISNKFVEHSRSADISVAVLKRSSESCENEKDDRI 360

Query: 361 ADDASTRVAGRRNGNGNKRMKGSTNVSHARTWEHIDHVIMNLPASALHFLDAFRGSIKKK 420
           ADDASTRVAGRRNGNGNKRMKGSTNVSHARTWEHIDHVIMNLPASALHFLDAFRGSIKKK
Sbjct: 361 ADDASTRVAGRRNGNGNKRMKGSTNVSHARTWEHIDHVIMNLPASALHFLDAFRGSIKKK 420

Query: 421 YWKGSLPWIHCYCFMRANENQDFIISVAESALNANIQDPIFHRVRDVAPNKAMYCLSFRL 471
           YWKGSLPWIHCYCFMRANENQDFIISVAESALNANIQDPIFHRVRDVAPNKAMYCLSFRL
Sbjct: 421 YWKGSLPWIHCYCFMRANENQDFIISVAESALNANIQDPIFHRVRDVAPNKAMYCLSFRL 480

BLAST of IVF0025767 vs. ExPASy TrEMBL
Match: A0A5A7V0B1 (tRNA (guanine(37)-N1)-methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold41G001350 PE=3 SV=1)

HSP 1 Score: 936.4 bits (2419), Expect = 4.6e-269
Identity = 469/500 (93.80%), Postives = 470/500 (94.00%), Query Frame = 0

Query: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60
           MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK
Sbjct: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60

Query: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWG---------------------- 120
           VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWG                      
Sbjct: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120

Query: 121 --------AGHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFE 180
                   AGHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFE
Sbjct: 121 GDNFVLCFAGHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFE 180

Query: 181 ILKGENDMVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFA 240
           ILKGENDMVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFA
Sbjct: 181 ILKGENDMVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFA 240

Query: 241 IPAAQKECIVYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNS 300
           IPAAQKECIVYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNS
Sbjct: 241 IPAAQKECIVYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNS 300

Query: 301 ETVTSILKASEEGIGGTNQESPLANVQVQEVQDIEISNKFVEHSRSADISVAVLKRSSES 360
           ETVTSILKASEEGIGGTNQESPLANVQVQEVQDIEISNKFVEHSRSADISVAVLKRSSES
Sbjct: 301 ETVTSILKASEEGIGGTNQESPLANVQVQEVQDIEISNKFVEHSRSADISVAVLKRSSES 360

Query: 361 CENEKEDRIADDASTRVAGRRNGNGNKRMKGSTNVSHARTWEHIDHVIMNLPASALHFLD 420
           CENEK+DRIADDASTRVAGRRNGNGNKRMKGSTNVSHARTWEHIDHVIMNLPASALHFLD
Sbjct: 361 CENEKDDRIADDASTRVAGRRNGNGNKRMKGSTNVSHARTWEHIDHVIMNLPASALHFLD 420

Query: 421 AFRGSIKKKYWKGSLPWIHCYCFMRANENQDFIISVAESALNANIQDPIFHRVRDVAPNK 471
           AFRGSIKKKYWKGSLPWIHCYCFMRANENQDFIISVAESALNANIQDPIFHRVRDVAPNK
Sbjct: 421 AFRGSIKKKYWKGSLPWIHCYCFMRANENQDFIISVAESALNANIQDPIFHRVRDVAPNK 480

BLAST of IVF0025767 vs. ExPASy TrEMBL
Match: A0A0A0LFW8 (tRNA (guanine(37)-N1)-methyltransferase OS=Cucumis sativus OX=3659 GN=Csa_2G005350 PE=3 SV=1)

HSP 1 Score: 901.0 bits (2327), Expect = 2.1e-258
Identity = 454/494 (91.90%), Postives = 463/494 (93.72%), Query Frame = 0

Query: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60
           MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK
Sbjct: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60

Query: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGAG-------------------- 120
           VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGA                     
Sbjct: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120

Query: 121 ---HVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGEND 180
              HVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGEND
Sbjct: 121 LLCHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGEND 180

Query: 181 MVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKE 240
           MVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKE
Sbjct: 181 MVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKE 240

Query: 241 CIVYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSETVTSIL 300
           CIVYANDLNPDS+RYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSE+VTSIL
Sbjct: 241 CIVYANDLNPDSIRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSESVTSIL 300

Query: 301 KASEEGIGGTNQESPLANVQVQEVQD-IEISNKFVEHSRSADISVAVLKRSSESCENEKE 360
           KA E+GIGGTNQESPLANVQVQEVQD I+ISNK VE SRSADISVAVLKRSSESCENEK+
Sbjct: 301 KACEKGIGGTNQESPLANVQVQEVQDYIKISNKSVEQSRSADISVAVLKRSSESCENEKD 360

Query: 361 DRIADDASTRVAGRRNGNGNKRMKGSTNVSHARTWEHIDHVIMNLPASALHFLDAFRGSI 420
           + IADDASTRVAGRRNGNGNKRMKGS +VSHARTWEH+DHVIMNLPASALHFLDAFRGSI
Sbjct: 361 NGIADDASTRVAGRRNGNGNKRMKGS-SVSHARTWEHVDHVIMNLPASALHFLDAFRGSI 420

Query: 421 KKKYWKGSLPWIHCYCFMRANENQDFIISVAESALNANIQDPIFHRVRDVAPNKAMYCLS 471
           KKKYWKGSLPWIHCYCFMRANENQDFIIS AESALNANIQDPIFHRVRDVAPNKAMYCLS
Sbjct: 421 KKKYWKGSLPWIHCYCFMRANENQDFIISEAESALNANIQDPIFHRVRDVAPNKAMYCLS 480

BLAST of IVF0025767 vs. ExPASy TrEMBL
Match: A0A6J1DUW2 (tRNA (guanine(37)-N1)-methyltransferase OS=Momordica charantia OX=3673 GN=LOC111024249 PE=3 SV=1)

HSP 1 Score: 814.7 bits (2103), Expect = 2.0e-232
Identity = 406/488 (83.20%), Postives = 434/488 (88.93%), Query Frame = 0

Query: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60
           MLDESKFDVHL LWALRIPRELCKAAIKILNGYLID+ R+KPVTEDPTCDKNRYVILSEK
Sbjct: 1   MLDESKFDVHLKLWALRIPRELCKAAIKILNGYLIDRARVKPVTEDPTCDKNRYVILSEK 60

Query: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGA--------------------- 120
           VQ PELS+IPE KLEELKGLC  E VPYSLTLGYSYWGA                     
Sbjct: 61  VQAPELSQIPEAKLEELKGLCEIELVPYSLTLGYSYWGADHILKQILPPRVEVPSSFETI 120

Query: 121 GHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMV 180
           GH+AHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVG+ITN+FRVPKFEIL GE+DMV
Sbjct: 121 GHIAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGTITNKFRVPKFEILMGEHDMV 180

Query: 181 TEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKECI 240
           TEVKQYGATFKLDY LVYWNSRLEHEH+RLVSLFQPGE+ICDMFAGIGPFAIPAAQKECI
Sbjct: 181 TEVKQYGATFKLDYGLVYWNSRLEHEHVRLVSLFQPGEIICDMFAGIGPFAIPAAQKECI 240

Query: 241 VYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSETVTSILKA 300
           VY NDLNPDSV+YLKVNAEINKVS RVHVYNLDARKFISQLMIVP NESNSE+VTS+LKA
Sbjct: 241 VYGNDLNPDSVQYLKVNAEINKVSDRVHVYNLDARKFISQLMIVPDNESNSESVTSVLKA 300

Query: 301 SEEGIGGTNQESPLANVQVQEVQD-IEISNKFVEHSRSADISVAVLKRSSESCENEKEDR 360
            ++GIGG+NQESP ANVQV+EVQ+  +IS++ VEHSRSADISV+VLKRSSESC  EK+D 
Sbjct: 301 CDKGIGGSNQESPSANVQVEEVQENSKISDETVEHSRSADISVSVLKRSSESC--EKDDG 360

Query: 361 IADDASTRVAGRRNGNGNKRMKGSTNVSHARTWEHIDHVIMNLPASALHFLDAFRGSIKK 420
           I DDAS  VAGR+  N NKRMKGS N+ HARTWEH+DHVIMNLPASAL FLDAFRGSI++
Sbjct: 361 IVDDASISVAGRKKRNANKRMKGS-NICHARTWEHVDHVIMNLPASALQFLDAFRGSIRR 420

Query: 421 KYWKGSLPWIHCYCFMRANENQDFIISVAESALNANIQDPIFHRVRDVAPNKAMYCLSFR 467
           KYWKGSLPWIHCYCFMRANENQDFIIS AESALN  IQDP+FHRVRDVAPNKAMYCLSFR
Sbjct: 421 KYWKGSLPWIHCYCFMRANENQDFIISEAESALNGKIQDPVFHRVRDVAPNKAMYCLSFR 480

BLAST of IVF0025767 vs. NCBI nr
Match: TYK10701.1 (tRNA (guanine(37)-N1)-methyltransferase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 951 bits (2459), Expect = 0.0
Identity = 470/470 (100.00%), Postives = 470/470 (100.00%), Query Frame = 0

Query: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60
           MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK
Sbjct: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60

Query: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGAGHVAHLNIHDELLPYKDVIAK 120
           VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGAGHVAHLNIHDELLPYKDVIAK
Sbjct: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGAGHVAHLNIHDELLPYKDVIAK 120

Query: 121 VIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMVTEVKQYGATFKLDYSLVYWNS 180
           VIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMVTEVKQYGATFKLDYSLVYWNS
Sbjct: 121 VIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMVTEVKQYGATFKLDYSLVYWNS 180

Query: 181 RLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKECIVYANDLNPDSVRYLKVNAEIN 240
           RLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKECIVYANDLNPDSVRYLKVNAEIN
Sbjct: 181 RLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKECIVYANDLNPDSVRYLKVNAEIN 240

Query: 241 KVSGRVHVYNLDARKFISQLMIVPPNESNSETVTSILKASEEGIGGTNQESPLANVQVQE 300
           KVSGRVHVYNLDARKFISQLMIVPPNESNSETVTSILKASEEGIGGTNQESPLANVQVQE
Sbjct: 241 KVSGRVHVYNLDARKFISQLMIVPPNESNSETVTSILKASEEGIGGTNQESPLANVQVQE 300

Query: 301 VQDIEISNKFVEHSRSADISVAVLKRSSESCENEKEDRIADDASTRVAGRRNGNGNKRMK 360
           VQDIEISNKFVEHSRSADISVAVLKRSSESCENEKEDRIADDASTRVAGRRNGNGNKRMK
Sbjct: 301 VQDIEISNKFVEHSRSADISVAVLKRSSESCENEKEDRIADDASTRVAGRRNGNGNKRMK 360

Query: 361 GSTNVSHARTWEHIDHVIMNLPASALHFLDAFRGSIKKKYWKGSLPWIHCYCFMRANENQ 420
           GSTNVSHARTWEHIDHVIMNLPASALHFLDAFRGSIKKKYWKGSLPWIHCYCFMRANENQ
Sbjct: 361 GSTNVSHARTWEHIDHVIMNLPASALHFLDAFRGSIKKKYWKGSLPWIHCYCFMRANENQ 420

Query: 421 DFIISVAESALNANIQDPIFHRVRDVAPNKAMYCLSFRLPEQCVNEDTTS 470
           DFIISVAESALNANIQDPIFHRVRDVAPNKAMYCLSFRLPEQCVNEDTTS
Sbjct: 421 DFIISVAESALNANIQDPIFHRVRDVAPNKAMYCLSFRLPEQCVNEDTTS 470

BLAST of IVF0025767 vs. NCBI nr
Match: XP_008458074.1 (PREDICTED: tRNA (guanine(37)-N1)-methyltransferase 2 [Cucumis melo])

HSP 1 Score: 936 bits (2420), Expect = 0.0
Identity = 468/491 (95.32%), Postives = 470/491 (95.72%), Query Frame = 0

Query: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60
           MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK
Sbjct: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60

Query: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGA--------------------- 120
           VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGA                     
Sbjct: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120

Query: 121 GHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMV 180
           GHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMV
Sbjct: 121 GHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMV 180

Query: 181 TEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKECI 240
           TEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKECI
Sbjct: 181 TEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKECI 240

Query: 241 VYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSETVTSILKA 300
           VYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFIS+LMIVPPNESNSETVTSILKA
Sbjct: 241 VYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFISRLMIVPPNESNSETVTSILKA 300

Query: 301 SEEGIGGTNQESPLANVQVQEVQDIEISNKFVEHSRSADISVAVLKRSSESCENEKEDRI 360
           SEEGIGGTNQESPLANVQVQEVQDIEISNKFVEHSRSADISVAVLKRSSESCENEK+DRI
Sbjct: 301 SEEGIGGTNQESPLANVQVQEVQDIEISNKFVEHSRSADISVAVLKRSSESCENEKDDRI 360

Query: 361 ADDASTRVAGRRNGNGNKRMKGSTNVSHARTWEHIDHVIMNLPASALHFLDAFRGSIKKK 420
           ADDASTRVAGRRNGNGNKRMKGSTNVSHARTWEHIDHVIMNLPASALHFLDAFRGSIKKK
Sbjct: 361 ADDASTRVAGRRNGNGNKRMKGSTNVSHARTWEHIDHVIMNLPASALHFLDAFRGSIKKK 420

Query: 421 YWKGSLPWIHCYCFMRANENQDFIISVAESALNANIQDPIFHRVRDVAPNKAMYCLSFRL 470
           YWKGSLPWIHCYCFMRANENQDFIISVAESALNANIQDPIFHRVRDVAPNKAMYCLSFRL
Sbjct: 421 YWKGSLPWIHCYCFMRANENQDFIISVAESALNANIQDPIFHRVRDVAPNKAMYCLSFRL 480

BLAST of IVF0025767 vs. NCBI nr
Match: KAA0061573.1 (tRNA (guanine(37)-N1)-methyltransferase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 934 bits (2415), Expect = 0.0
Identity = 469/500 (93.80%), Postives = 470/500 (94.00%), Query Frame = 0

Query: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60
           MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK
Sbjct: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60

Query: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGA--------------------- 120
           VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGA                     
Sbjct: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120

Query: 121 ---------GHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFE 180
                    GHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFE
Sbjct: 121 GDNFVLCFAGHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFE 180

Query: 181 ILKGENDMVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFA 240
           ILKGENDMVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFA
Sbjct: 181 ILKGENDMVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFA 240

Query: 241 IPAAQKECIVYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNS 300
           IPAAQKECIVYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNS
Sbjct: 241 IPAAQKECIVYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNS 300

Query: 301 ETVTSILKASEEGIGGTNQESPLANVQVQEVQDIEISNKFVEHSRSADISVAVLKRSSES 360
           ETVTSILKASEEGIGGTNQESPLANVQVQEVQDIEISNKFVEHSRSADISVAVLKRSSES
Sbjct: 301 ETVTSILKASEEGIGGTNQESPLANVQVQEVQDIEISNKFVEHSRSADISVAVLKRSSES 360

Query: 361 CENEKEDRIADDASTRVAGRRNGNGNKRMKGSTNVSHARTWEHIDHVIMNLPASALHFLD 420
           CENEK+DRIADDASTRVAGRRNGNGNKRMKGSTNVSHARTWEHIDHVIMNLPASALHFLD
Sbjct: 361 CENEKDDRIADDASTRVAGRRNGNGNKRMKGSTNVSHARTWEHIDHVIMNLPASALHFLD 420

Query: 421 AFRGSIKKKYWKGSLPWIHCYCFMRANENQDFIISVAESALNANIQDPIFHRVRDVAPNK 470
           AFRGSIKKKYWKGSLPWIHCYCFMRANENQDFIISVAESALNANIQDPIFHRVRDVAPNK
Sbjct: 421 AFRGSIKKKYWKGSLPWIHCYCFMRANENQDFIISVAESALNANIQDPIFHRVRDVAPNK 480

BLAST of IVF0025767 vs. NCBI nr
Match: XP_011648665.1 (tRNA (guanine(37)-N1)-methyltransferase 2 isoform X2 [Cucumis sativus])

HSP 1 Score: 903 bits (2333), Expect = 0.0
Identity = 455/492 (92.48%), Postives = 464/492 (94.31%), Query Frame = 0

Query: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60
           MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK
Sbjct: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60

Query: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGA--------------------- 120
           VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGA                     
Sbjct: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120

Query: 121 GHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMV 180
           GHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMV
Sbjct: 121 GHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMV 180

Query: 181 TEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKECI 240
           TEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKECI
Sbjct: 181 TEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKECI 240

Query: 241 VYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSETVTSILKA 300
           VYANDLNPDS+RYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSE+VTSILKA
Sbjct: 241 VYANDLNPDSIRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSESVTSILKA 300

Query: 301 SEEGIGGTNQESPLANVQVQEVQD-IEISNKFVEHSRSADISVAVLKRSSESCENEKEDR 360
            E+GIGGTNQESPLANVQVQEVQD I+ISNK VE SRSADISVAVLKRSSESCENEK++ 
Sbjct: 301 CEKGIGGTNQESPLANVQVQEVQDYIKISNKSVEQSRSADISVAVLKRSSESCENEKDNG 360

Query: 361 IADDASTRVAGRRNGNGNKRMKGSTNVSHARTWEHIDHVIMNLPASALHFLDAFRGSIKK 420
           IADDASTRVAGRRNGNGNKRMKGS+ VSHARTWEH+DHVIMNLPASALHFLDAFRGSIKK
Sbjct: 361 IADDASTRVAGRRNGNGNKRMKGSS-VSHARTWEHVDHVIMNLPASALHFLDAFRGSIKK 420

Query: 421 KYWKGSLPWIHCYCFMRANENQDFIISVAESALNANIQDPIFHRVRDVAPNKAMYCLSFR 470
           KYWKGSLPWIHCYCFMRANENQDFIIS AESALNANIQDPIFHRVRDVAPNKAMYCLSFR
Sbjct: 421 KYWKGSLPWIHCYCFMRANENQDFIISEAESALNANIQDPIFHRVRDVAPNKAMYCLSFR 480

BLAST of IVF0025767 vs. NCBI nr
Match: XP_031736378.1 (tRNA (guanine(37)-N1)-methyltransferase 2 isoform X1 [Cucumis sativus])

HSP 1 Score: 897 bits (2318), Expect = 0.0
Identity = 455/496 (91.73%), Postives = 464/496 (93.55%), Query Frame = 0

Query: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60
           MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK
Sbjct: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60

Query: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGA--------------------- 120
           VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGA                     
Sbjct: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120

Query: 121 GHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMV 180
           GHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMV
Sbjct: 121 GHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMV 180

Query: 181 TEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKECI 240
           TEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKECI
Sbjct: 181 TEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKECI 240

Query: 241 VYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSETVTSILKA 300
           VYANDLNPDS+RYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSE+VTSILKA
Sbjct: 241 VYANDLNPDSIRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSESVTSILKA 300

Query: 301 SEEGIGGTNQESPLAN----VQVQEVQD-IEISNKFVEHSRSADISVAVLKRSSESCENE 360
            E+GIGGTNQESPLAN    VQVQEVQD I+ISNK VE SRSADISVAVLKRSSESCENE
Sbjct: 301 CEKGIGGTNQESPLANGMPTVQVQEVQDYIKISNKSVEQSRSADISVAVLKRSSESCENE 360

Query: 361 KEDRIADDASTRVAGRRNGNGNKRMKGSTNVSHARTWEHIDHVIMNLPASALHFLDAFRG 420
           K++ IADDASTRVAGRRNGNGNKRMKGS+ VSHARTWEH+DHVIMNLPASALHFLDAFRG
Sbjct: 361 KDNGIADDASTRVAGRRNGNGNKRMKGSS-VSHARTWEHVDHVIMNLPASALHFLDAFRG 420

Query: 421 SIKKKYWKGSLPWIHCYCFMRANENQDFIISVAESALNANIQDPIFHRVRDVAPNKAMYC 470
           SIKKKYWKGSLPWIHCYCFMRANENQDFIIS AESALNANIQDPIFHRVRDVAPNKAMYC
Sbjct: 421 SIKKKYWKGSLPWIHCYCFMRANENQDFIISEAESALNANIQDPIFHRVRDVAPNKAMYC 480

BLAST of IVF0025767 vs. TAIR 10
Match: AT3G56120.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 568.9 bits (1465), Expect = 3.7e-162
Identity = 292/490 (59.59%), Postives = 351/490 (71.63%), Query Frame = 0

Query: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60
           M DESKFDV+L LWALRIPRELCK+A +ILNGY+++ PRIKP+TEDPTC+K R VILSE 
Sbjct: 1   MFDESKFDVNLKLWALRIPRELCKSASRILNGYMLNMPRIKPITEDPTCEKTRLVILSES 60

Query: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGA--------------------- 120
           V+  +LSEIPE KL +LK L   E VP+S+TLGYSYW A                     
Sbjct: 61  VKNADLSEIPEEKLNQLKKLSELEVVPHSVTLGYSYWSADHLLKQILPDGLDIPSSFETI 120

Query: 121 GHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMV 180
           GH+AHLN+HDELLP+KDVIAKVIYDKNYPRIKT+VNKVG+I+NEFRVPKFE+L GEN M 
Sbjct: 121 GHIAHLNLHDELLPFKDVIAKVIYDKNYPRIKTIVNKVGTISNEFRVPKFEVLAGENGME 180

Query: 181 TEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKECI 240
           TEVKQYGA FKLDY LVYWNSRLEHEH+RL SLF+PGE +CDMFAGIGPFAIPAAQK C 
Sbjct: 181 TEVKQYGARFKLDYGLVYWNSRLEHEHMRLSSLFKPGETVCDMFAGIGPFAIPAAQKGCF 240

Query: 241 VYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSETVTSILKA 300
           VYANDLNPDSVRYLK+NA+ NKV   + V+N+DARKF S LM V   E N ++V    K 
Sbjct: 241 VYANDLNPDSVRYLKINAKFNKVDDLICVHNMDARKFFSHLMAVSTCEDNLQSVADNDKT 300

Query: 301 SEEGI--GGTNQESPLANVQVQEVQDIEISNKFVEHSRSADISVAVLKRSSESCENEKED 360
            E  +  GG    S          ++I  SN        A I+  +      S   + E+
Sbjct: 301 KEAAVSRGGETNSSG---------EEIRESN--------ASINEPLGANKKPSGTTKTEN 360

Query: 361 RIADDASTRVAGRRNGNGNKRMKGSTNVSHARTWEHIDHVIMNLPASALHFLDAFRGSIK 420
            +  D  +       G+ NKR++  T +  A+ WEHIDHVIMNLPASAL FLD+F   I+
Sbjct: 361 GVGKDCKS-----IEGHANKRLR-QTLLPIAKPWEHIDHVIMNLPASALQFLDSFSNVIQ 420

Query: 421 KKYWKGSLPWIHCYCFMRANENQDFIISVAESALNANIQDPIFHRVRDVAPNKAMYCLSF 468
           KKYWKG LP IHCYCF+RA+E  +FII+ AE+AL  +I+DP+FH+VRDVAPNKAM+CLSF
Sbjct: 421 KKYWKGPLPLIHCYCFIRASETTEFIIAEAETALKFHIEDPVFHKVRDVAPNKAMFCLSF 467

BLAST of IVF0025767 vs. TAIR 10
Match: AT4G27340.1 (Met-10+ like family protein )

HSP 1 Score: 183.7 bits (465), Expect = 3.4e-46
Identity = 97/259 (37.45%), Postives = 147/259 (56.76%), Query Frame = 0

Query: 53  RYVILSEKVQTPELSEIPE------MKLEELKGLCMFERVPYSLTLGYSYWG-------- 112
           R ++L EK    E+ ++PE       + +       FE V   LTL Y YW         
Sbjct: 274 RLLLLDEKYSGEEVQDLPEAIKVLFAEAKMADASLSFELVKCRLTLFYDYWPMIEILEAV 333

Query: 113 -------------AGHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFR 172
                         GH+AHLN+ DE LPYK +IAKV+ DKN P+I+TVVNK+  I N+FR
Sbjct: 334 LPKGMIVPSAFEMVGHIAHLNLRDEHLPYKRLIAKVVLDKNQPKIQTVVNKIDPIHNDFR 393

Query: 173 VPKFEILKGENDMVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAG 232
             + E+L G + +VT V + G  F +D + VYWNS+L  E  RL+  F   +V+CD+FAG
Sbjct: 394 TMQLEVLAGNHSLVTLVVENGLRFHVDLARVYWNSKLGTERQRLLLGFDQNDVVCDVFAG 453

Query: 233 IGPFAIPAAQKECIVYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPP 285
           +GP A+ AA+    VYANDLNP +V +++ N+ +NK+  R+ ++N+D R+FI  +     
Sbjct: 454 VGPIALAAARIVKRVYANDLNPHAVEFMEQNSVVNKLEKRIEIFNMDGRRFIKAMFSSEK 513

BLAST of IVF0025767 vs. TAIR 10
Match: AT4G04670.1 (Met-10+ like family protein / kelch repeat-containing protein )

HSP 1 Score: 55.5 bits (132), Expect = 1.4e-07
Identity = 51/193 (26.42%), Postives = 87/193 (45.08%), Query Frame = 0

Query: 142 NEFRVPKFEILKGENDMVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICD 201
           N  R    EIL G+N  V   ++ G  +  D +   ++     E +R+ ++    EV+ D
Sbjct: 785 NGTRDSTLEILVGDNGWVNH-RENGILYSFDATKCMFSWGNLSEKLRMGNMACENEVVVD 844

Query: 202 MFAGIGPFAIP--AAQKECIVYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDAR----K 261
           +FAGIG F +P     K  +VYA + NP ++  L+ N E N VS R  +   D R    K
Sbjct: 845 LFAGIGYFVLPFLVRAKAKLVYACEWNPHAIEALRRNVEANSVSERCIILEGDNRITAPK 904

Query: 262 FIS---QLMIVPPNESNSETVTSIL-------------KASEEGIGGTNQESPLANVQVQ 313
            ++    L ++P +E +  T    L             K S+E   G +    L+++   
Sbjct: 905 GVADRVNLGLIPSSEGSWVTAIQALRPEGGILHVHGNVKDSDESSWGEHVTKTLSDIARA 964

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
F6HH453.3e-17964.27tRNA (guanine(37)-N1)-methyltransferase 2 OS=Vitis vinifera OX=29760 GN=VIT_11s0... [more]
Q93YU65.2e-16159.59tRNA (guanine(37)-N1)-methyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=At3... [more]
C5XX797.5e-16061.75tRNA (guanine(37)-N1)-methyltransferase OS=Sorghum bicolor OX=4558 GN=Sb04g02539... [more]
A9T6G55.0e-10344.16tRNA (guanine(37)-N1)-methyltransferase OS=Physcomitrium patens OX=3218 GN=PHYPA... [more]
F4NUJ61.8e-6532.51tRNA (guanine(37)-N1)-methyltransferase OS=Batrachochytrium dendrobatidis (strai... [more]
Match NameE-valueIdentityDescription
A0A5D3CFY13.6e-274100.00tRNA (guanine(37)-N1)-methyltransferase OS=Cucumis melo var. makuwa OX=1194695 G... [more]
A0A1S3C7L31.2e-26995.32tRNA (guanine(37)-N1)-methyltransferase OS=Cucumis melo OX=3656 GN=LOC103497610 ... [more]
A0A5A7V0B14.6e-26993.80tRNA (guanine(37)-N1)-methyltransferase OS=Cucumis melo var. makuwa OX=1194695 G... [more]
A0A0A0LFW82.1e-25891.90tRNA (guanine(37)-N1)-methyltransferase OS=Cucumis sativus OX=3659 GN=Csa_2G0053... [more]
A0A6J1DUW22.0e-23283.20tRNA (guanine(37)-N1)-methyltransferase OS=Momordica charantia OX=3673 GN=LOC111... [more]
Match NameE-valueIdentityDescription
TYK10701.10.0100.00tRNA (guanine(37)-N1)-methyltransferase 2 [Cucumis melo var. makuwa][more]
XP_008458074.10.095.32PREDICTED: tRNA (guanine(37)-N1)-methyltransferase 2 [Cucumis melo][more]
KAA0061573.10.093.80tRNA (guanine(37)-N1)-methyltransferase 2 [Cucumis melo var. makuwa][more]
XP_011648665.10.092.48tRNA (guanine(37)-N1)-methyltransferase 2 isoform X2 [Cucumis sativus][more]
XP_031736378.10.091.73tRNA (guanine(37)-N1)-methyltransferase 2 isoform X1 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
AT3G56120.13.7e-16259.59S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT4G27340.13.4e-4637.45Met-10+ like family protein [more]
AT4G04670.11.4e-0726.42Met-10+ like family protein / kelch repeat-containing protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 159..459
e-value: 1.5E-57
score: 196.2
NoneNo IPR availableGENE3D3.30.300.110coord: 86..156
e-value: 1.3E-18
score: 68.8
NoneNo IPR availablePANTHERPTHR23245TRNA METHYLTRANSFERASEcoord: 2..465
NoneNo IPR availablePANTHERPTHR23245:SF36TRNA (GUANINE(37)-N1)-METHYLTRANSFERASEcoord: 2..465
NoneNo IPR availableCDDcd02440AdoMet_MTasescoord: 199..256
e-value: 3.79393E-4
score: 37.7947
IPR030382SAM-dependent methyltransferase TRM5/TYW2-typePFAMPF02475Met_10coord: 96..411
e-value: 1.5E-64
score: 217.4
IPR030382SAM-dependent methyltransferase TRM5/TYW2-typePROSITEPS51684SAM_MT_TRM5_TYW2coord: 96..461
score: 103.33989
IPR025792tRNA (guanine(37)-N(1))-methyltransferase, eukaryoticHAMAPMF_03152TRM5coord: 2..461
score: 23.483767
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 97..461

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
IVF0025767.2IVF0025767.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0030488 tRNA methylation
cellular_component GO:0005759 mitochondrial matrix
cellular_component GO:0005634 nucleus
molecular_function GO:0052906 tRNA (guanine(37)-N(1))-methyltransferase activity
molecular_function GO:0009019 tRNA (guanine-N1-)-methyltransferase activity