IVF0025660 (gene) Melon (IVF77) v1

BLAST of IVF0025660 vs. ExPASy Swiss-Prot
Match: Q8RXN5 (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase OS=Arabidopsis thaliana OX=3702 GN=TYW1 PE=2 SV=1)

HSP 1 Score: 966.5 bits (2497), Expect = 1.5e-280
Identity = 471/644 (73.14%), Postives = 538/644 (83.54%), Query Frame = 0

Query: 4   SSRSAKLTILALLSASTLYCFYKSRRLRKLK-LSLNP--TLSSRKPKLFFISQTGTSKAL 63
           SS   +L  +ALLSA+T YC +K RRL+ LK LSLNP  TL + + K+FFISQTGT+KAL
Sbjct: 5   SSVRVRLAFVALLSATTFYCIHKYRRLKHLKNLSLNPSSTLKASRGKIFFISQTGTAKAL 64

Query: 64  AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 123
           A RL  +  SN + FD+VDP  YEPEDL KETLVLF+ASTW+ G PP + +FL NWL ES
Sbjct: 65  AQRLHELCASNDIAFDIVDPHSYEPEDLPKETLVLFIASTWDGGKPPKNGEFLVNWLGES 124

Query: 124 AEDFRVGSLLLSQCKFSVFGVGSRVYGETYNAVARDFSKRMKALGAKEILPIGEGDVDGG 183
           AEDFRVGSLLLS CKF+VFGVGSR YGE+YNAVA++ S RM  LG  E++P+GEGDVD G
Sbjct: 125 AEDFRVGSLLLSDCKFAVFGVGSRAYGESYNAVAKELSSRMIGLGGLEMIPVGEGDVDDG 184

Query: 184 DIDKCFDDWSRKLLKVVKVDAEGNGVELCSGIAGDSDAVSVEEEY-----EEEDDDLGEE 243
           ++D+ F DW   +++V+K    G   +  +G++    AV  + EY     EE++D+  + 
Sbjct: 185 ELDRAFQDWCDGVIRVLK----GGSAQETNGVSQQIGAVEDDLEYYDSTDEEDEDNDADG 244

Query: 244 DIVDLEDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICR 303
            IVDLEDIAGK PS+++  V  T  K++GKK MVTPVIRASL KQGYKIIGSHSGVKICR
Sbjct: 245 GIVDLEDIAGKAPSKRNGVVKVT--KVDGKKEMVTPVIRASLTKQGYKIIGSHSGVKICR 304

Query: 304 WTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDP 363
           WTKSQLRGRGGCYKHSFYGIESHRCME TPSLACANKCVFCWRHHTNPVGKSWQW+MD+P
Sbjct: 305 WTKSQLRGRGGCYKHSFYGIESHRCMETTPSLACANKCVFCWRHHTNPVGKSWQWKMDEP 364

Query: 364 LDIVNSAIDLHTKMIKQMKGVPGVTQEKLEEGLTPRHCALSLVGEPIMYPEINTLVDELH 423
             IV  A+DLH  MIKQMKGVPGVT EKL+EGL PRHCALSLVGEPIMYPEIN LVDELH
Sbjct: 365 SVIVKGALDLHKNMIKQMKGVPGVTPEKLQEGLNPRHCALSLVGEPIMYPEINALVDELH 424

Query: 424 QRRISTFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKA 483
            RRISTFLVTNAQFP+KI ++KP+TQLYVSVDAATKESLKAIDRPLF DFWERFIDSLKA
Sbjct: 425 GRRISTFLVTNAQFPEKILMMKPITQLYVSVDAATKESLKAIDRPLFADFWERFIDSLKA 484

Query: 484 LNEKQQRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVP 543
           L EKQQRTVYRLTLVKGWNTE++DAY NLFSIG+PDF+EIKGVTYCGSS TSKLTMENVP
Sbjct: 485 LQEKQQRTVYRLTLVKGWNTEELDAYFNLFSIGKPDFIEIKGVTYCGSSATSKLTMENVP 544

Query: 544 WHSDVKSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVA 603
           WH+DVK+FSEAL+ KS GEYEVACEH HSCCVLL +T+KF+VDG+WFTWIDYEKFHDLVA
Sbjct: 545 WHTDVKAFSEALSLKSNGEYEVACEHAHSCCVLLGRTEKFKVDGKWFTWIDYEKFHDLVA 604

Query: 604 SGKPFDSKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKP 640
           SG+PF S DYMA TPSWAVYG+ EGGFDP Q RY+KER+H PKP
Sbjct: 605 SGEPFTSTDYMAQTPSWAVYGAQEGGFDPGQLRYKKERNHPPKP 642

BLAST of IVF0025660 vs. ExPASy Swiss-Prot
Match: Q8H8N3 (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase OS=Oryza sativa subsp. japonica OX=39947 GN=TYW1 PE=2 SV=1)

HSP 1 Score: 880.6 bits (2274), Expect = 1.1e-254
Identity = 435/642 (67.76%), Postives = 510/642 (79.44%), Query Frame = 0

Query: 4   SSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLS--SRKPKLFFISQTGTSKALA 63
           +S ++ L +L LLS+S+++  YKS RLR+      P     +  P L + S TGTSKALA
Sbjct: 12  ASTTSHLALLLLLSSSSVFFLYKSLRLRRNNPPSPPPGQGPAPTPTLLYASATGTSKALA 71

Query: 64  HRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSESA 123
             L   L   G+     D   ++P+DL    L+L V  T + G PPP A FLA WL ESA
Sbjct: 72  AGLSRRLAEAGVTAHPADAAAFDPDDLPSLPLLLLVLPTHDGGAPPPAAAFLARWLEESA 131

Query: 124 EDFRVGSLLLSQCKFSVFGVGSRVYGETYNAVARDFSKRMKALGAKEILPIGEGDVDGGD 183
            DFR G+ LLS  +F+VFGVGSR YGET+NA AR FS+ ++ALGA E++ +GEGDVDGGD
Sbjct: 132 ADFRAGAALLSGLRFAVFGVGSRAYGETFNAAARSFSRWLRALGAAEVVAVGEGDVDGGD 191

Query: 184 IDKCFDDWSRKLLKVVKVDAEGNGVELCSGIAGDSDAVSVEEEYEEEDDDLGEEDI---- 243
           ++  F++W  ++++VVK      G E+  G  G+SD    E E EE DDD  EE++    
Sbjct: 192 LEVVFEEWCGRVVRVVK------GEEIGEGHNGESDGFD-ELEEEESDDDDDEEEVDGGE 251

Query: 244 VDLEDIAGKGP-SRKSTNVVE---TNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKI 303
           VD+EDIAGK P +R+    VE   +NG  NG + MVTP+IR SLEKQGYKIIGSHSGVKI
Sbjct: 252 VDMEDIAGKAPAARRRNGKVEGALSNGGENGVRDMVTPIIRTSLEKQGYKIIGSHSGVKI 311

Query: 304 CRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMD 363
           CRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSW+W+MD
Sbjct: 312 CRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWKWKMD 371

Query: 364 DPLDIVNSAIDLHTKMIKQMKGVPGVTQEKLEEGLTPRHCALSLVGEPIMYPEINTLVDE 423
           DPLDIVN+AID HTKM+KQMKGVPGV  E+L EGL+PRHCALSLVGEPIMYPEIN L+DE
Sbjct: 372 DPLDIVNAAIDQHTKMVKQMKGVPGVKPERLAEGLSPRHCALSLVGEPIMYPEINVLIDE 431

Query: 424 LHQRRISTFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSL 483
           LH+R ISTFLVTNAQFPDKIK LKP+TQLYVSVDAATKESLKA+DRPLF DFWERF+DSL
Sbjct: 432 LHRRHISTFLVTNAQFPDKIKTLKPITQLYVSVDAATKESLKAVDRPLFSDFWERFLDSL 491

Query: 484 KALNEKQQRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMEN 543
           K+L++K QRTVYRLTLVKGWN E+ID Y+ L S+G+PDF+EIKGVTYCGSS TSKLTMEN
Sbjct: 492 KSLHDKDQRTVYRLTLVKGWNAEEIDGYAKLLSLGQPDFIEIKGVTYCGSSATSKLTMEN 551

Query: 544 VPWHSDVKSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDL 603
           VPWHSDVK FSEALA KS G YEVACEH HSCCVLLAK DKF+++G+W TWIDY++FH+L
Sbjct: 552 VPWHSDVKDFSEALALKSGGVYEVACEHAHSCCVLLAKVDKFKINGKWHTWIDYDRFHEL 611

Query: 604 VASGKPFDSKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHH 636
           V SGKPF S+DYMA TPSWAVYG++EGGFDPDQSRY+KER H
Sbjct: 612 VTSGKPFRSQDYMALTPSWAVYGAEEGGFDPDQSRYKKERRH 646

BLAST of IVF0025660 vs. ExPASy Swiss-Prot
Match: Q2KHP8 (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase TYW1 OS=Xenopus laevis OX=8355 GN=tyw1 PE=2 SV=1)

HSP 1 Score: 628.6 bits (1620), Expect = 7.6e-179
Identity = 335/677 (49.48%), Postives = 436/677 (64.40%), Query Frame = 0

Query: 30  LRKLKLSLNPTLSSRKP--------KLFFISQTGTSKALAHRLLNVLNSNGLVFDLVDPK 89
           L K K   + +L+S K         K+F+ SQTGTSK  AH L   +   GL  +L++ K
Sbjct: 59  LLKTKALKSDSLTSNKEQKVYVAGVKIFYGSQTGTSKGFAHLLAEEVTLLGLPVELINMK 118

Query: 90  DYEPED-LFKET----LVLFVASTWEDGGPPPHAKFLANWLSESAEDFRVGSLLLSQCKF 149
           +Y+P+D L +ET    + +F+ +T+ DG PP  A++   WL E++ DFR G   L   ++
Sbjct: 119 EYDPDDNLVEETTSKNICVFLVATYTDGKPPESAEWFCKWLEEASNDFRFGKTYLKGMRY 178

Query: 150 SVFGVGSRVYGETYNAVARDFSKRMKALGAKEILPIGEGDVD-----GGDIDKCFDDWSR 209
           +VFG+G+ VY   YN V ++  K +  L A  ++   EGD +      G I+  F+ W R
Sbjct: 179 AVFGLGNSVYSTHYNTVGKNIDKWLWMLSANRVMTRAEGDCNVVKSKHGSIESDFEAWKR 238

Query: 210 KLLKVVKVDAEGNGVELCSG--------IAGDSDAVSVEEEYEEEDDDLGEED------- 269
           K L  +K    G   + CSG            S   SVEEE EEE+     ED       
Sbjct: 239 KFLNRLKALLSGE-KKPCSGKCKKGKCKSKKKSSIESVEEEEEEEEKHSEHEDTEDDTFE 298

Query: 270 ----------------IVDLEDIAGKGPS--RKSTNVVETNGKLNGKKV----------- 329
                           ++D+ED+ GK  S  +KS    ++N +L GK +           
Sbjct: 299 TSSDSEPEEHGEPGSGLIDVEDL-GKAMSNMKKSKREHDSNTEL-GKSLQGDEAKEKEEP 358

Query: 330 --MVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATP 389
             M+TP ++ +L KQGY++IGSHSGVK+CRWTKS LRGRGGCYKH+FYGIESHRCME TP
Sbjct: 359 REMITPALQEALTKQGYRLIGSHSGVKLCRWTKSMLRGRGGCYKHTFYGIESHRCMETTP 418

Query: 390 SLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVNSAIDLHTKMIKQMKGVPGVTQEKLE 449
           SLACANKCVFCWRHHTNPVG  W+W+MD P  I+  AI  H  MIKQ KGVPGV  E+ E
Sbjct: 419 SLACANKCVFCWRHHTNPVGTEWRWKMDQPEMILEEAILNHQNMIKQFKGVPGVKPERFE 478

Query: 450 EGLTPRHCALSLVGEPIMYPEINTLVDELHQRRISTFLVTNAQFPDKIKLLKPVTQLYVS 509
           EGL  +HCALSLVGEPIMYPEINT +  LH + IS+FLVTNAQFP++I+ L+PVTQLYVS
Sbjct: 479 EGLAVKHCALSLVGEPIMYPEINTFLRLLHNQHISSFLVTNAQFPEEIRSLEPVTQLYVS 538

Query: 510 VDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQQRTVYRLTLVKGWNTEDIDAYSNLF 569
           VDA+TK+SLK IDRPLF DFW+RFIDSLKAL EKQQRTVYRLTLVK WN +++ AY++L 
Sbjct: 539 VDASTKDSLKKIDRPLFKDFWQRFIDSLKALAEKQQRTVYRLTLVKAWNVDELKAYADLV 598

Query: 570 SIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDVKSFSEALAAKSQGEYEVACEHVHSC 629
           ++G PDF+E+KGVTYCG S+ S LTM NVPWH +V  F E L      +YEVACEH HS 
Sbjct: 599 ALGLPDFIEVKGVTYCGESSASNLTMANVPWHEEVIRFVEEL-VDLLPDYEVACEHEHSN 658

Query: 630 CVLLAKTDKFRVDGQWFTWIDYEKFHDLVAS------GKPFDSKDYMAPTPSWAVYGSDE 637
           C+L+A   KF+++G+W TWIDYE+F +L+ +       + F + DY+A TP WA++GS E
Sbjct: 659 CMLIAH-KKFKINGEWCTWIDYERFQELIQAFGESKGSRTFTALDYVAKTPEWALFGSRE 718

BLAST of IVF0025660 vs. ExPASy Swiss-Prot
Match: Q08C92 (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase TYW1 OS=Danio rerio OX=7955 GN=tyw1 PE=2 SV=1)

HSP 1 Score: 623.2 bits (1606), Expect = 3.2e-177
Identity = 318/649 (49.00%), Postives = 427/649 (65.79%), Query Frame = 0

Query: 47  KLFFISQTGTSKALAHRLLNVLNSNGLVFDLVDPKDYEPEDLFKE-----TLVLFVASTW 106
           K+F+ SQTGT+K  A  L   + + G+  +++D KD++PED   E      + +F+ +T+
Sbjct: 80  KVFYGSQTGTAKGFAKELAEDVIAQGIQCEVIDMKDFDPEDRLAEECTSKIICVFLVATY 139

Query: 107 EDGGPPPHAKFLANWLSESAEDFRVGSLLLSQCKFSVFGVGSRVYGETYNAVARDFSKRM 166
            DG P   A++   WL E++ DFR G   L   +++VFG+G+ VY   +N V++   K +
Sbjct: 140 TDGQPTESAEWFCKWLEEASTDFRYGKTYLKGMRYAVFGLGNSVYVGHFNTVSKSIDKWL 199

Query: 167 KALGAKEILPIGEGDVD-----GGDIDKCFDDWSRKLLKVVKVDAEGNGVELCSG----- 226
             L A  I+  GEGD +      G +   F  W  K L  ++  A+G   + CSG     
Sbjct: 200 WMLSAARIMTRGEGDCNVVKSRHGSVQADFQVWKGKFLNRLQALAKGE-KKACSGNCKKA 259

Query: 227 ----------IAGDSDAVSVEEEYEEE------DDDLGEED------IVDLEDIAG---- 286
                      A D+ +++ +   EEE      D++   ED      ++D+ED+      
Sbjct: 260 SCKNKKKHKEEAEDNHSLAEKNNSEEELMESSSDEESSSEDEKSHGSVIDMEDLGNVMNH 319

Query: 287 --KGPSR-----KSTNVVETNGKLNG-----KKVMVTPVIRASLEKQGYKIIGSHSGVKI 346
             K   R     + +  V+ NG+        ++ M+TP +R SL KQGYK+IGSHSGVK+
Sbjct: 320 MKKAKQRMEEDEEDSQRVKQNGERKSECEEERREMITPALRDSLTKQGYKLIGSHSGVKL 379

Query: 347 CRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMD 406
            RWTKS LRGRGGCYKH+FYGIESHRCME TPSLACANKCVFCWRHHTNPVG  W+W+MD
Sbjct: 380 RRWTKSMLRGRGGCYKHTFYGIESHRCMETTPSLACANKCVFCWRHHTNPVGTEWRWKMD 439

Query: 407 DPLDIVNSAIDLHTKMIKQMKGVPGVTQEKLEEGLTPRHCALSLVGEPIMYPEINTLVDE 466
               I+  A++ H  MI+Q +GVPGV  E+ EEGLT +HCALSLVGEPIMYPEIN+ +  
Sbjct: 440 PAEKIIQEAMENHRNMIRQFRGVPGVRPERFEEGLTVKHCALSLVGEPIMYPEINSFLKL 499

Query: 467 LHQRRISTFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSL 526
           LHQ+ IS+FLVTNAQFP++I+ L PVTQLYVSVDA+TK+SLK IDRPLF DFW+RF+DSL
Sbjct: 500 LHQQNISSFLVTNAQFPEEIRSLVPVTQLYVSVDASTKDSLKKIDRPLFKDFWQRFLDSL 559

Query: 527 KALNEKQQRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMEN 586
           +AL EKQQRTVYRLTLVK WN +++ AY++L ++G+PDF+E+KGVTYCG S+ S LTM N
Sbjct: 560 RALGEKQQRTVYRLTLVKAWNVDELKAYADLIALGQPDFIEVKGVTYCGESSASSLTMAN 619

Query: 587 VPWHSDVKSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDL 637
           VPWH +V  F + L A    +YE+ACEH HS C+LLA   KF+VDG+W+TWIDYE+F +L
Sbjct: 620 VPWHEEVIYFVQQL-ANLLPDYEIACEHEHSNCLLLA-NHKFKVDGEWWTWIDYERFQEL 679

BLAST of IVF0025660 vs. ExPASy Swiss-Prot
Match: Q9NV66 (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase TYW1 OS=Homo sapiens OX=9606 GN=TYW1 PE=2 SV=2)

HSP 1 Score: 613.2 bits (1580), Expect = 3.3e-174
Identity = 311/650 (47.85%), Postives = 415/650 (63.85%), Query Frame = 0

Query: 47  KLFFISQTGTSKALAHRLLNVLNSNGLVFDLVDPKDYEPED-----LFKETLVLFVASTW 106
           K+F+ SQTGT+K  A  L   + S  L   +++ K+Y+P+D     +  + + +F+ +T+
Sbjct: 80  KIFYGSQTGTAKGFATVLAEAVTSLDLPVAIINLKEYDPDDHLIEEVTSKNVCVFLVATY 139

Query: 107 EDGGPPPHAKFLANWLSESAEDFRVGSLLLSQCKFSVFGVGSRVYGETYNAVARDFSKRM 166
            DG P   A++   WL E++ DFR G   L   +++VFG+G+  Y   +N V ++  K +
Sbjct: 140 TDGLPTESAEWFCKWLEEASIDFRFGKTYLKGMRYAVFGLGNSAYASHFNKVGKNVDKWL 199

Query: 167 KALGAKEILPIGEGDVD-----GGDIDKCFDDWSRKLLKVVKVDAEGNGVELCSGI---- 226
             LGA  ++  GEGD D      G I+  F  W  K +  ++   +G   + C G     
Sbjct: 200 WMLGAHRVMSRGEGDCDVVKSKHGSIEADFRAWKTKFISQLQALQKGERKKSCGGHCKKG 259

Query: 227 ---------------AGDSDAVSVEEEYEEE------DDDLGEED------IVDLEDIAG 286
                          + + D +   +  EEE      +++ G ED      IVD+ED+  
Sbjct: 260 KCESHQHGSEEREEGSHEQDELHHRDTEEEEPFESSSEEEFGGEDHQSLNSIVDVEDLGK 319

Query: 287 -----KGPSRKSTNVVETNGKLNG--------KKVMVTPVIRASLEKQGYKIIGSHSGVK 346
                K   R+     E +G            ++ M+TP +R +L KQGY++IGSHSGVK
Sbjct: 320 IMDHVKKEKREKEQQEEKSGLFRNMGRNEDGERRAMITPALREALTKQGYQLIGSHSGVK 379

Query: 347 ICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEM 406
           +CRWTKS LRGRGGCYKH+FYGIESHRCME TPSLACANKCVFCWRHHTNPVG  W+W+M
Sbjct: 380 LCRWTKSMLRGRGGCYKHTFYGIESHRCMETTPSLACANKCVFCWRHHTNPVGTEWRWKM 439

Query: 407 DDPLDIVNSAIDLHTKMIKQMKGVPGVTQEKLEEGLTPRHCALSLVGEPIMYPEINTLVD 466
           D P  I+  AI+ H  MIKQ KGVPGV  E+ EEG+T +HCALSLVGEPIMYPEIN  + 
Sbjct: 440 DQPEMILKEAIENHQNMIKQFKGVPGVKAERFEEGMTVKHCALSLVGEPIMYPEINRFLK 499

Query: 467 ELHQRRISTFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDS 526
            LHQ +IS+FLVTNAQFP +I+ L+PVTQLYVSVDA+TK+SLK IDRPLF DFW+RF+DS
Sbjct: 500 LLHQCKISSFLVTNAQFPAEIRNLEPVTQLYVSVDASTKDSLKKIDRPLFKDFWQRFLDS 559

Query: 527 LKALNEKQQRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTME 586
           LKAL  KQQRTVYRLTLVK WN +++ AY+ L S+G PDF+E+KGVTYCG S+ S LTM 
Sbjct: 560 LKALAVKQQRTVYRLTLVKAWNVDELQAYAQLVSLGNPDFIEVKGVTYCGESSASSLTMA 619

Query: 587 NVPWHSDVKSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHD 637
           +VPWH +V  F   L      EYE+ACEH HS C+L+A   KF++ G+W+TWIDY +F +
Sbjct: 620 HVPWHEEVVQFVHELVDLIP-EYEIACEHEHSNCLLIAHR-KFKIGGEWWTWIDYNRFQE 679

BLAST of IVF0025660 vs. ExPASy TrEMBL
Match: A0A5A7VC83 (tRNA 4-demethylwyosine synthase (AdoMet-dependent) OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G005790 PE=3 SV=1)

HSP 1 Score: 1308.5 bits (3385), Expect = 0.0e+00
Identity = 639/641 (99.69%), Postives = 640/641 (99.84%), Query Frame = 0

Query: 1   MSFSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLSSRKPKLFFISQTGTSKAL 60
           MSFSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLSSRKPKLFFISQTGTSKAL
Sbjct: 1   MSFSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLSSRKPKLFFISQTGTSKAL 60

Query: 61  AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 120
           AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES
Sbjct: 61  AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 120

Query: 121 AEDFRVGSLLLSQCKFSVFGVGSRVYGETYNAVARDFSKRMKALGAKEILPIGEGDVDGG 180
           AEDFRVGSLLLSQCKFSVFGVGSRVYGETYNAVARDFSKRMKALGAKEILPIGEGDVDGG
Sbjct: 121 AEDFRVGSLLLSQCKFSVFGVGSRVYGETYNAVARDFSKRMKALGAKEILPIGEGDVDGG 180

Query: 181 DIDKCFDDWSRKLLKVVKVDAEGNGVELCSGIAGDSDAVSVEEEYEEEDDDLGEEDIVDL 240
           DIDKCFDDWSRKLLKVVKVDAEGNGVELCSGIAGDSDAVSVEEEYEEEDDDLGEEDIVDL
Sbjct: 181 DIDKCFDDWSRKLLKVVKVDAEGNGVELCSGIAGDSDAVSVEEEYEEEDDDLGEEDIVDL 240

Query: 241 EDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ 300
           EDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ
Sbjct: 241 EDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ 300

Query: 301 LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVN 360
           LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVN
Sbjct: 301 LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVN 360

Query: 361 SAIDLHTKMIKQMKGVPGVTQEKLEEGLTPRHCALSLVGEPIMYPEINTLVDELHQRRIS 420
           SAIDLHTKMIKQMKGVPGVTQEKLEEGL+PRHCA SLVGEPIMYPEINTLVDELHQRRIS
Sbjct: 361 SAIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCAFSLVGEPIMYPEINTLVDELHQRRIS 420

Query: 421 TFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQ 480
           TFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQ
Sbjct: 421 TFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQ 480

Query: 481 QRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDV 540
           QRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDV
Sbjct: 481 QRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDV 540

Query: 541 KSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVASGKPF 600
           KSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVASGKPF
Sbjct: 541 KSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVASGKPF 600

Query: 601 DSKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS 642
           DSKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS
Sbjct: 601 DSKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS 641

BLAST of IVF0025660 vs. ExPASy TrEMBL
Match: A0A1S3BAY2 (tRNA 4-demethylwyosine synthase (AdoMet-dependent) OS=Cucumis melo OX=3656 GN=LOC103487965 PE=3 SV=1)

HSP 1 Score: 1307.4 bits (3382), Expect = 0.0e+00
Identity = 638/641 (99.53%), Postives = 641/641 (100.00%), Query Frame = 0

Query: 1   MSFSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLSSRKPKLFFISQTGTSKAL 60
           MSFSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLSSRKPKLFFISQTGTSKAL
Sbjct: 1   MSFSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLSSRKPKLFFISQTGTSKAL 60

Query: 61  AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 120
           AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES
Sbjct: 61  AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 120

Query: 121 AEDFRVGSLLLSQCKFSVFGVGSRVYGETYNAVARDFSKRMKALGAKEILPIGEGDVDGG 180
           AEDFRVGSLLLSQCKFSVFGVGSRVYGETYNAVARDFSKRMK+LGAKEILPIGEGDVDGG
Sbjct: 121 AEDFRVGSLLLSQCKFSVFGVGSRVYGETYNAVARDFSKRMKSLGAKEILPIGEGDVDGG 180

Query: 181 DIDKCFDDWSRKLLKVVKVDAEGNGVELCSGIAGDSDAVSVEEEYEEEDDDLGEEDIVDL 240
           DIDKCFDDWSRKLLKVVKVDAEGNGVELCSGIAGDSDAVSVEEEYEEEDDDLGEEDIVDL
Sbjct: 181 DIDKCFDDWSRKLLKVVKVDAEGNGVELCSGIAGDSDAVSVEEEYEEEDDDLGEEDIVDL 240

Query: 241 EDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ 300
           EDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ
Sbjct: 241 EDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ 300

Query: 301 LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVN 360
           LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVN
Sbjct: 301 LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVN 360

Query: 361 SAIDLHTKMIKQMKGVPGVTQEKLEEGLTPRHCALSLVGEPIMYPEINTLVDELHQRRIS 420
           SAIDLHTKMIKQMKGVPGVTQEKLEEGL+PRHCALSLVGEPIMYPEINTLVDELHQRRIS
Sbjct: 361 SAIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHQRRIS 420

Query: 421 TFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQ 480
           TFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQ
Sbjct: 421 TFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQ 480

Query: 481 QRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDV 540
           QRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDV
Sbjct: 481 QRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDV 540

Query: 541 KSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVASGKPF 600
           KSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVASGKPF
Sbjct: 541 KSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVASGKPF 600

Query: 601 DSKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS 642
           DSKDY+APTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS
Sbjct: 601 DSKDYIAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS 641

BLAST of IVF0025660 vs. ExPASy TrEMBL
Match: A0A0A0LPA4 (tRNA 4-demethylwyosine synthase (AdoMet-dependent) OS=Cucumis sativus OX=3659 GN=Csa_2G361620 PE=3 SV=1)

HSP 1 Score: 1284.6 bits (3323), Expect = 0.0e+00
Identity = 627/641 (97.82%), Postives = 636/641 (99.22%), Query Frame = 0

Query: 1   MSFSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLSSRKPKLFFISQTGTSKAL 60
           MS SSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTL SRKPKLFFISQTGTSKAL
Sbjct: 1   MSLSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLPSRKPKLFFISQTGTSKAL 60

Query: 61  AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 120
           AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES
Sbjct: 61  AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 120

Query: 121 AEDFRVGSLLLSQCKFSVFGVGSRVYGETYNAVARDFSKRMKALGAKEILPIGEGDVDGG 180
           AEDFRVGSLLLSQCKFSVFGVGSRVYGET+NAVARDFSKR+KALGAKEILPIGEGDVDGG
Sbjct: 121 AEDFRVGSLLLSQCKFSVFGVGSRVYGETFNAVARDFSKRLKALGAKEILPIGEGDVDGG 180

Query: 181 DIDKCFDDWSRKLLKVVKVDAEGNGVELCSGIAGDSDAVSVEEEYEEEDDDLGEEDIVDL 240
           DIDKCFDDWSRKLLKV+K DAEGNGVELCSGIAGDSDAVSVEE+ EEEDDDLGEEDIVDL
Sbjct: 181 DIDKCFDDWSRKLLKVMKFDAEGNGVELCSGIAGDSDAVSVEED-EEEDDDLGEEDIVDL 240

Query: 241 EDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ 300
           EDIAGKGPSRKSTN+VETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ
Sbjct: 241 EDIAGKGPSRKSTNIVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ 300

Query: 301 LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVN 360
           LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPL+IVN
Sbjct: 301 LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLEIVN 360

Query: 361 SAIDLHTKMIKQMKGVPGVTQEKLEEGLTPRHCALSLVGEPIMYPEINTLVDELHQRRIS 420
           SAIDLHTKMIKQMKGVPGVTQEKLEEGL+PRHCALSLVGEPIMYPEINTLVDELH+RRIS
Sbjct: 361 SAIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIS 420

Query: 421 TFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQ 480
           TFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQ
Sbjct: 421 TFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQ 480

Query: 481 QRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDV 540
           QRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSS TSKLTMENVPWHSDV
Sbjct: 481 QRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSTTSKLTMENVPWHSDV 540

Query: 541 KSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVASGKPF 600
           KSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDY+KFHDLVASGKPF
Sbjct: 541 KSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYDKFHDLVASGKPF 600

Query: 601 DSKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS 642
           DSKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS
Sbjct: 601 DSKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS 640

BLAST of IVF0025660 vs. ExPASy TrEMBL
Match: A0A6J1K9S7 (tRNA 4-demethylwyosine synthase (AdoMet-dependent) OS=Cucurbita maxima OX=3661 GN=LOC111492394 PE=3 SV=1)

HSP 1 Score: 1229.9 bits (3181), Expect = 0.0e+00
Identity = 600/641 (93.60%), Postives = 619/641 (96.57%), Query Frame = 0

Query: 1   MSFSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLSSRKPKLFFISQTGTSKAL 60
           MSFSSRSAKL +LALLSASTLYCFYKSRRLRKLKLSLNPT  SR+PKLFFISQTGTSKAL
Sbjct: 1   MSFSSRSAKLALLALLSASTLYCFYKSRRLRKLKLSLNPTGCSRRPKLFFISQTGTSKAL 60

Query: 61  AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 120
           AHRLLNVLNSN L FD+VDPKD+EPEDLFKE LVLFVASTWEDGGPPPHAKFLANWLSES
Sbjct: 61  AHRLLNVLNSNELAFDIVDPKDFEPEDLFKEALVLFVASTWEDGGPPPHAKFLANWLSES 120

Query: 121 AEDFRVGSLLLSQCKFSVFGVGSRVYGETYNAVARDFSKRMKALGAKEILPIGEGDVDGG 180
           AEDFRVGSLLLSQCKF+VFGVGSR YGET+NAVARDFSKRMKALGAKEIL +GEGDVDGG
Sbjct: 121 AEDFRVGSLLLSQCKFAVFGVGSRAYGETFNAVARDFSKRMKALGAKEILAVGEGDVDGG 180

Query: 181 DIDKCFDDWSRKLLKVVKVDAEGNGVELCSGIAGDSDAVSVEEEYEEEDDDLGEEDIVDL 240
           DIDKCFDDWSR+LLKV+KVDA G G +L SGI  D DA SVEE  ++ED D GEEDIVDL
Sbjct: 181 DIDKCFDDWSRQLLKVLKVDAVGTGADLYSGIDEDFDAESVEE--DDEDADPGEEDIVDL 240

Query: 241 EDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ 300
           EDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ
Sbjct: 241 EDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ 300

Query: 301 LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVN 360
           LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVN
Sbjct: 301 LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVN 360

Query: 361 SAIDLHTKMIKQMKGVPGVTQEKLEEGLTPRHCALSLVGEPIMYPEINTLVDELHQRRIS 420
           SAIDLHTKMIKQMKGVPGVTQEKLEEGL+PRHCALSLVGEPIMYPEINTLVDELH+RRIS
Sbjct: 361 SAIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIS 420

Query: 421 TFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQ 480
           TFLVTNAQFPDKIK LKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALN+KQ
Sbjct: 421 TFLVTNAQFPDKIKSLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNDKQ 480

Query: 481 QRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDV 540
           QRTVYRLTLVKGWNTEDIDAYSNLFS+G+PDFVEIKGVTYCGSSNTSKLTMENVPWHSDV
Sbjct: 481 QRTVYRLTLVKGWNTEDIDAYSNLFSLGKPDFVEIKGVTYCGSSNTSKLTMENVPWHSDV 540

Query: 541 KSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVASGKPF 600
           KSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVASGKPF
Sbjct: 541 KSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVASGKPF 600

Query: 601 DSKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS 642
           DSKDYMAPTPSWAVYG+DEGGFDP QSRYRKERHH+PKPTS
Sbjct: 601 DSKDYMAPTPSWAVYGADEGGFDPVQSRYRKERHHRPKPTS 639

BLAST of IVF0025660 vs. ExPASy TrEMBL
Match: A0A6J1HDZ5 (tRNA 4-demethylwyosine synthase (AdoMet-dependent) OS=Cucurbita moschata OX=3662 GN=LOC111462691 PE=3 SV=1)

HSP 1 Score: 1220.7 bits (3157), Expect = 0.0e+00
Identity = 595/641 (92.82%), Postives = 617/641 (96.26%), Query Frame = 0

Query: 1   MSFSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLSSRKPKLFFISQTGTSKAL 60
           MS SSRSAKL +LALLSASTLYCFYKSRRLRKLKLSLNPT  SR+PKLFFISQTGTSKAL
Sbjct: 1   MSLSSRSAKLALLALLSASTLYCFYKSRRLRKLKLSLNPTGCSRRPKLFFISQTGTSKAL 60

Query: 61  AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 120
           AHRLLNVLNSN L FD+VDPKD+EPEDLFKETLVLFVASTWEDGGPPPHAKFLA+WLSES
Sbjct: 61  AHRLLNVLNSNELAFDIVDPKDFEPEDLFKETLVLFVASTWEDGGPPPHAKFLASWLSES 120

Query: 121 AEDFRVGSLLLSQCKFSVFGVGSRVYGETYNAVARDFSKRMKALGAKEILPIGEGDVDGG 180
           AEDFRVGSLLLSQCKF+VFGVGSR YGET+NAVARDFSKRMKALGAKEIL +GEGDVDGG
Sbjct: 121 AEDFRVGSLLLSQCKFAVFGVGSRAYGETFNAVARDFSKRMKALGAKEILAVGEGDVDGG 180

Query: 181 DIDKCFDDWSRKLLKVVKVDAEGNGVELCSGIAGDSDAVSVEEEYEEEDDDLGEEDIVDL 240
           DIDKCFDDWSR+LLKV+KVDA G G +L SGI  D DA SVEE  ++ED D GEEDIVDL
Sbjct: 181 DIDKCFDDWSRQLLKVLKVDAVGTGADLYSGIDEDFDAESVEE--DDEDADPGEEDIVDL 240

Query: 241 EDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ 300
           EDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ
Sbjct: 241 EDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ 300

Query: 301 LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVN 360
           LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVN
Sbjct: 301 LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVN 360

Query: 361 SAIDLHTKMIKQMKGVPGVTQEKLEEGLTPRHCALSLVGEPIMYPEINTLVDELHQRRIS 420
           SAIDLH KMIKQMKGVPGVTQEKLEEGL+PRHCALSLVGEPIMYPEINTLVDELH+RRIS
Sbjct: 361 SAIDLHKKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIS 420

Query: 421 TFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQ 480
           TFLVTNAQFPDKIK LKPVTQLYVSVDAATK+SLKAIDRPLFGDFWERFIDSLKALN+KQ
Sbjct: 421 TFLVTNAQFPDKIKSLKPVTQLYVSVDAATKDSLKAIDRPLFGDFWERFIDSLKALNDKQ 480

Query: 481 QRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDV 540
           QRTVYRLTLVKGWNTEDIDAYSNLFS+G+PDFVEIKGVTYCGSSNTSKLTMENVPWHSDV
Sbjct: 481 QRTVYRLTLVKGWNTEDIDAYSNLFSLGKPDFVEIKGVTYCGSSNTSKLTMENVPWHSDV 540

Query: 541 KSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVASGKPF 600
           KSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFR+DGQWFTWIDYEKFH LVASGKPF
Sbjct: 541 KSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRIDGQWFTWIDYEKFHALVASGKPF 600

Query: 601 DSKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS 642
           DSKDYMAPTPSWAVYG+DEGGFDP QSRYRKERHH+PKPTS
Sbjct: 601 DSKDYMAPTPSWAVYGADEGGFDPVQSRYRKERHHRPKPTS 639

BLAST of IVF0025660 vs. NCBI nr
Match: KAA0065238.1 (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase [Cucumis melo var. makuwa])

HSP 1 Score: 1301 bits (3367), Expect = 0.0
Identity = 639/641 (99.69%), Postives = 640/641 (99.84%), Query Frame = 0

Query: 1   MSFSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLSSRKPKLFFISQTGTSKAL 60
           MSFSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLSSRKPKLFFISQTGTSKAL
Sbjct: 1   MSFSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLSSRKPKLFFISQTGTSKAL 60

Query: 61  AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 120
           AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES
Sbjct: 61  AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 120

Query: 121 AEDFRVGSLLLSQCKFSVFGVGSRVYGETYNAVARDFSKRMKALGAKEILPIGEGDVDGG 180
           AEDFRVGSLLLSQCKFSVFGVGSRVYGETYNAVARDFSKRMKALGAKEILPIGEGDVDGG
Sbjct: 121 AEDFRVGSLLLSQCKFSVFGVGSRVYGETYNAVARDFSKRMKALGAKEILPIGEGDVDGG 180

Query: 181 DIDKCFDDWSRKLLKVVKVDAEGNGVELCSGIAGDSDAVSVEEEYEEEDDDLGEEDIVDL 240
           DIDKCFDDWSRKLLKVVKVDAEGNGVELCSGIAGDSDAVSVEEEYEEEDDDLGEEDIVDL
Sbjct: 181 DIDKCFDDWSRKLLKVVKVDAEGNGVELCSGIAGDSDAVSVEEEYEEEDDDLGEEDIVDL 240

Query: 241 EDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ 300
           EDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ
Sbjct: 241 EDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ 300

Query: 301 LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVN 360
           LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVN
Sbjct: 301 LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVN 360

Query: 361 SAIDLHTKMIKQMKGVPGVTQEKLEEGLTPRHCALSLVGEPIMYPEINTLVDELHQRRIS 420
           SAIDLHTKMIKQMKGVPGVTQEKLEEGL+PRHCA SLVGEPIMYPEINTLVDELHQRRIS
Sbjct: 361 SAIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCAFSLVGEPIMYPEINTLVDELHQRRIS 420

Query: 421 TFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQ 480
           TFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQ
Sbjct: 421 TFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQ 480

Query: 481 QRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDV 540
           QRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDV
Sbjct: 481 QRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDV 540

Query: 541 KSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVASGKPF 600
           KSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVASGKPF
Sbjct: 541 KSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVASGKPF 600

Query: 601 DSKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS 641
           DSKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS
Sbjct: 601 DSKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS 641

BLAST of IVF0025660 vs. NCBI nr
Match: XP_008444711.1 (PREDICTED: S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase [Cucumis melo])

HSP 1 Score: 1300 bits (3364), Expect = 0.0
Identity = 638/641 (99.53%), Postives = 641/641 (100.00%), Query Frame = 0

Query: 1   MSFSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLSSRKPKLFFISQTGTSKAL 60
           MSFSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLSSRKPKLFFISQTGTSKAL
Sbjct: 1   MSFSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLSSRKPKLFFISQTGTSKAL 60

Query: 61  AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 120
           AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES
Sbjct: 61  AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 120

Query: 121 AEDFRVGSLLLSQCKFSVFGVGSRVYGETYNAVARDFSKRMKALGAKEILPIGEGDVDGG 180
           AEDFRVGSLLLSQCKFSVFGVGSRVYGETYNAVARDFSKRMK+LGAKEILPIGEGDVDGG
Sbjct: 121 AEDFRVGSLLLSQCKFSVFGVGSRVYGETYNAVARDFSKRMKSLGAKEILPIGEGDVDGG 180

Query: 181 DIDKCFDDWSRKLLKVVKVDAEGNGVELCSGIAGDSDAVSVEEEYEEEDDDLGEEDIVDL 240
           DIDKCFDDWSRKLLKVVKVDAEGNGVELCSGIAGDSDAVSVEEEYEEEDDDLGEEDIVDL
Sbjct: 181 DIDKCFDDWSRKLLKVVKVDAEGNGVELCSGIAGDSDAVSVEEEYEEEDDDLGEEDIVDL 240

Query: 241 EDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ 300
           EDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ
Sbjct: 241 EDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ 300

Query: 301 LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVN 360
           LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVN
Sbjct: 301 LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVN 360

Query: 361 SAIDLHTKMIKQMKGVPGVTQEKLEEGLTPRHCALSLVGEPIMYPEINTLVDELHQRRIS 420
           SAIDLHTKMIKQMKGVPGVTQEKLEEGL+PRHCALSLVGEPIMYPEINTLVDELHQRRIS
Sbjct: 361 SAIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHQRRIS 420

Query: 421 TFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQ 480
           TFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQ
Sbjct: 421 TFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQ 480

Query: 481 QRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDV 540
           QRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDV
Sbjct: 481 QRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDV 540

Query: 541 KSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVASGKPF 600
           KSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVASGKPF
Sbjct: 541 KSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVASGKPF 600

Query: 601 DSKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS 641
           DSKDY+APTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS
Sbjct: 601 DSKDYIAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS 641

BLAST of IVF0025660 vs. NCBI nr
Match: XP_004152698.1 (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase [Cucumis sativus] >KGN62597.1 hypothetical protein Csa_021988 [Cucumis sativus])

HSP 1 Score: 1277 bits (3304), Expect = 0.0
Identity = 627/641 (97.82%), Postives = 636/641 (99.22%), Query Frame = 0

Query: 1   MSFSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLSSRKPKLFFISQTGTSKAL 60
           MS SSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTL SRKPKLFFISQTGTSKAL
Sbjct: 1   MSLSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLPSRKPKLFFISQTGTSKAL 60

Query: 61  AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 120
           AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES
Sbjct: 61  AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 120

Query: 121 AEDFRVGSLLLSQCKFSVFGVGSRVYGETYNAVARDFSKRMKALGAKEILPIGEGDVDGG 180
           AEDFRVGSLLLSQCKFSVFGVGSRVYGET+NAVARDFSKR+KALGAKEILPIGEGDVDGG
Sbjct: 121 AEDFRVGSLLLSQCKFSVFGVGSRVYGETFNAVARDFSKRLKALGAKEILPIGEGDVDGG 180

Query: 181 DIDKCFDDWSRKLLKVVKVDAEGNGVELCSGIAGDSDAVSVEEEYEEEDDDLGEEDIVDL 240
           DIDKCFDDWSRKLLKV+K DAEGNGVELCSGIAGDSDAVSVEE+ EEEDDDLGEEDIVDL
Sbjct: 181 DIDKCFDDWSRKLLKVMKFDAEGNGVELCSGIAGDSDAVSVEED-EEEDDDLGEEDIVDL 240

Query: 241 EDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ 300
           EDIAGKGPSRKSTN+VETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ
Sbjct: 241 EDIAGKGPSRKSTNIVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ 300

Query: 301 LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVN 360
           LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPL+IVN
Sbjct: 301 LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLEIVN 360

Query: 361 SAIDLHTKMIKQMKGVPGVTQEKLEEGLTPRHCALSLVGEPIMYPEINTLVDELHQRRIS 420
           SAIDLHTKMIKQMKGVPGVTQEKLEEGL+PRHCALSLVGEPIMYPEINTLVDELH+RRIS
Sbjct: 361 SAIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIS 420

Query: 421 TFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQ 480
           TFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQ
Sbjct: 421 TFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQ 480

Query: 481 QRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDV 540
           QRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSS TSKLTMENVPWHSDV
Sbjct: 481 QRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSTTSKLTMENVPWHSDV 540

Query: 541 KSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVASGKPF 600
           KSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDY+KFHDLVASGKPF
Sbjct: 541 KSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYDKFHDLVASGKPF 600

Query: 601 DSKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS 641
           DSKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS
Sbjct: 601 DSKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS 640

BLAST of IVF0025660 vs. NCBI nr
Match: XP_038884961.1 (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase [Benincasa hispida] >XP_038884963.1 S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase [Benincasa hispida])

HSP 1 Score: 1251 bits (3236), Expect = 0.0
Identity = 613/641 (95.63%), Postives = 632/641 (98.60%), Query Frame = 0

Query: 1   MSFSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLSSRKPKLFFISQTGTSKAL 60
           MSFSSRSAKLT+LALLSASTLYCFYKSRRLRKLKLSLNPTLSSR+PKLFFISQTGTSKAL
Sbjct: 1   MSFSSRSAKLTLLALLSASTLYCFYKSRRLRKLKLSLNPTLSSRRPKLFFISQTGTSKAL 60

Query: 61  AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 120
           AHRLLNVLNSN LVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES
Sbjct: 61  AHRLLNVLNSNELVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 120

Query: 121 AEDFRVGSLLLSQCKFSVFGVGSRVYGETYNAVARDFSKRMKALGAKEILPIGEGDVDGG 180
           AEDFRVGSLLLS+CKF+VFGVGSR YGET+NAVAR+FSKRMKALGAKEIL +G+GDVDGG
Sbjct: 121 AEDFRVGSLLLSECKFAVFGVGSRAYGETFNAVARNFSKRMKALGAKEILAVGKGDVDGG 180

Query: 181 DIDKCFDDWSRKLLKVVKVDAEGNGVELCSGIAGDSDAVSVEEEYEEEDDDLGEEDIVDL 240
           DID+CFDDWSR+LLKV+KVDA GNGV+L SGI GDSDA SVEE+ EEED DLGEEDIVDL
Sbjct: 181 DIDRCFDDWSRQLLKVLKVDAVGNGVDLYSGIGGDSDAESVEED-EEEDADLGEEDIVDL 240

Query: 241 EDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ 300
           EDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ
Sbjct: 241 EDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ 300

Query: 301 LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVN 360
           LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVN
Sbjct: 301 LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVN 360

Query: 361 SAIDLHTKMIKQMKGVPGVTQEKLEEGLTPRHCALSLVGEPIMYPEINTLVDELHQRRIS 420
           SAIDLHTKMIKQMKGVPGVTQEKL+EGL+PRHCALSLVGEPIMYPEINTLVDELH+RRIS
Sbjct: 361 SAIDLHTKMIKQMKGVPGVTQEKLDEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIS 420

Query: 421 TFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQ 480
           TFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALN+KQ
Sbjct: 421 TFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNDKQ 480

Query: 481 QRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDV 540
           QRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDV
Sbjct: 481 QRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDV 540

Query: 541 KSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVASGKPF 600
           KSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVASGKPF
Sbjct: 541 KSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVASGKPF 600

Query: 601 DSKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS 641
           DSKDYMAPTPSWAVYGSDEGGFDP+QSRYRKERHH+PKPTS
Sbjct: 601 DSKDYMAPTPSWAVYGSDEGGFDPNQSRYRKERHHRPKPTS 640

BLAST of IVF0025660 vs. NCBI nr
Match: XP_022997485.1 (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase [Cucurbita maxima])

HSP 1 Score: 1222 bits (3162), Expect = 0.0
Identity = 600/641 (93.60%), Postives = 619/641 (96.57%), Query Frame = 0

Query: 1   MSFSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLSSRKPKLFFISQTGTSKAL 60
           MSFSSRSAKL +LALLSASTLYCFYKSRRLRKLKLSLNPT  SR+PKLFFISQTGTSKAL
Sbjct: 1   MSFSSRSAKLALLALLSASTLYCFYKSRRLRKLKLSLNPTGCSRRPKLFFISQTGTSKAL 60

Query: 61  AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 120
           AHRLLNVLNSN L FD+VDPKD+EPEDLFKE LVLFVASTWEDGGPPPHAKFLANWLSES
Sbjct: 61  AHRLLNVLNSNELAFDIVDPKDFEPEDLFKEALVLFVASTWEDGGPPPHAKFLANWLSES 120

Query: 121 AEDFRVGSLLLSQCKFSVFGVGSRVYGETYNAVARDFSKRMKALGAKEILPIGEGDVDGG 180
           AEDFRVGSLLLSQCKF+VFGVGSR YGET+NAVARDFSKRMKALGAKEIL +GEGDVDGG
Sbjct: 121 AEDFRVGSLLLSQCKFAVFGVGSRAYGETFNAVARDFSKRMKALGAKEILAVGEGDVDGG 180

Query: 181 DIDKCFDDWSRKLLKVVKVDAEGNGVELCSGIAGDSDAVSVEEEYEEEDDDLGEEDIVDL 240
           DIDKCFDDWSR+LLKV+KVDA G G +L SGI  D DA SVEE+  +ED D GEEDIVDL
Sbjct: 181 DIDKCFDDWSRQLLKVLKVDAVGTGADLYSGIDEDFDAESVEED--DEDADPGEEDIVDL 240

Query: 241 EDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ 300
           EDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ
Sbjct: 241 EDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ 300

Query: 301 LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVN 360
           LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVN
Sbjct: 301 LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVN 360

Query: 361 SAIDLHTKMIKQMKGVPGVTQEKLEEGLTPRHCALSLVGEPIMYPEINTLVDELHQRRIS 420
           SAIDLHTKMIKQMKGVPGVTQEKLEEGL+PRHCALSLVGEPIMYPEINTLVDELH+RRIS
Sbjct: 361 SAIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIS 420

Query: 421 TFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQ 480
           TFLVTNAQFPDKIK LKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALN+KQ
Sbjct: 421 TFLVTNAQFPDKIKSLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNDKQ 480

Query: 481 QRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDV 540
           QRTVYRLTLVKGWNTEDIDAYSNLFS+G+PDFVEIKGVTYCGSSNTSKLTMENVPWHSDV
Sbjct: 481 QRTVYRLTLVKGWNTEDIDAYSNLFSLGKPDFVEIKGVTYCGSSNTSKLTMENVPWHSDV 540

Query: 541 KSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVASGKPF 600
           KSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVASGKPF
Sbjct: 541 KSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVASGKPF 600

Query: 601 DSKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS 641
           DSKDYMAPTPSWAVYG+DEGGFDP QSRYRKERHH+PKPTS
Sbjct: 601 DSKDYMAPTPSWAVYGADEGGFDPVQSRYRKERHHRPKPTS 639

BLAST of IVF0025660 vs. TAIR 10
Match: AT1G75200.1 (flavodoxin family protein / radical SAM domain-containing protein )

HSP 1 Score: 966.5 bits (2497), Expect = 1.1e-281
Identity = 471/644 (73.14%), Postives = 538/644 (83.54%), Query Frame = 0

Query: 4   SSRSAKLTILALLSASTLYCFYKSRRLRKLK-LSLNP--TLSSRKPKLFFISQTGTSKAL 63
           SS   +L  +ALLSA+T YC +K RRL+ LK LSLNP  TL + + K+FFISQTGT+KAL
Sbjct: 5   SSVRVRLAFVALLSATTFYCIHKYRRLKHLKNLSLNPSSTLKASRGKIFFISQTGTAKAL 64

Query: 64  AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 123
           A RL  +  SN + FD+VDP  YEPEDL KETLVLF+ASTW+ G PP + +FL NWL ES
Sbjct: 65  AQRLHELCASNDIAFDIVDPHSYEPEDLPKETLVLFIASTWDGGKPPKNGEFLVNWLGES 124

Query: 124 AEDFRVGSLLLSQCKFSVFGVGSRVYGETYNAVARDFSKRMKALGAKEILPIGEGDVDGG 183
           AEDFRVGSLLLS CKF+VFGVGSR YGE+YNAVA++ S RM  LG  E++P+GEGDVD G
Sbjct: 125 AEDFRVGSLLLSDCKFAVFGVGSRAYGESYNAVAKELSSRMIGLGGLEMIPVGEGDVDDG 184

Query: 184 DIDKCFDDWSRKLLKVVKVDAEGNGVELCSGIAGDSDAVSVEEEY-----EEEDDDLGEE 243
           ++D+ F DW   +++V+K    G   +  +G++    AV  + EY     EE++D+  + 
Sbjct: 185 ELDRAFQDWCDGVIRVLK----GGSAQETNGVSQQIGAVEDDLEYYDSTDEEDEDNDADG 244

Query: 244 DIVDLEDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICR 303
            IVDLEDIAGK PS+++  V  T  K++GKK MVTPVIRASL KQGYKIIGSHSGVKICR
Sbjct: 245 GIVDLEDIAGKAPSKRNGVVKVT--KVDGKKEMVTPVIRASLTKQGYKIIGSHSGVKICR 304

Query: 304 WTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDP 363
           WTKSQLRGRGGCYKHSFYGIESHRCME TPSLACANKCVFCWRHHTNPVGKSWQW+MD+P
Sbjct: 305 WTKSQLRGRGGCYKHSFYGIESHRCMETTPSLACANKCVFCWRHHTNPVGKSWQWKMDEP 364

Query: 364 LDIVNSAIDLHTKMIKQMKGVPGVTQEKLEEGLTPRHCALSLVGEPIMYPEINTLVDELH 423
             IV  A+DLH  MIKQMKGVPGVT EKL+EGL PRHCALSLVGEPIMYPEIN LVDELH
Sbjct: 365 SVIVKGALDLHKNMIKQMKGVPGVTPEKLQEGLNPRHCALSLVGEPIMYPEINALVDELH 424

Query: 424 QRRISTFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKA 483
            RRISTFLVTNAQFP+KI ++KP+TQLYVSVDAATKESLKAIDRPLF DFWERFIDSLKA
Sbjct: 425 GRRISTFLVTNAQFPEKILMMKPITQLYVSVDAATKESLKAIDRPLFADFWERFIDSLKA 484

Query: 484 LNEKQQRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVP 543
           L EKQQRTVYRLTLVKGWNTE++DAY NLFSIG+PDF+EIKGVTYCGSS TSKLTMENVP
Sbjct: 485 LQEKQQRTVYRLTLVKGWNTEELDAYFNLFSIGKPDFIEIKGVTYCGSSATSKLTMENVP 544

Query: 544 WHSDVKSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVA 603
           WH+DVK+FSEAL+ KS GEYEVACEH HSCCVLL +T+KF+VDG+WFTWIDYEKFHDLVA
Sbjct: 545 WHTDVKAFSEALSLKSNGEYEVACEHAHSCCVLLGRTEKFKVDGKWFTWIDYEKFHDLVA 604

Query: 604 SGKPFDSKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKP 640
           SG+PF S DYMA TPSWAVYG+ EGGFDP Q RY+KER+H PKP
Sbjct: 605 SGEPFTSTDYMAQTPSWAVYGAQEGGFDPGQLRYKKERNHPPKP 642