IVF0025404 (gene) Melon (IVF77) v1

Overview


Name	IVF0025404
Type	gene
Organism	Cucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
Description	Trypsin inhibitor 1
Location	chr07: 24283767 .. 24284280 (-)
RNA-Seq Expression	IVF0025404
Synteny	IVF0025404

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAGAGAGAGAGAGAAAGCAAAGAAAGAGAGAAAAAAAAAATGGAGTGGAAAAAAGTTGCTTTAGTTGCAGTAATGGGGATGTTGGTTATGGCTACTTTTACAGAGTCGGTCGGTTTGGGGGTGGATGAGGAGATCATTCAATTAGTTTCGGACGGAGTGAATGAGTACTCACAAAATATTGGAAAGAAAAATGTAGGCTGCCCTCGAATCCTTATGAAGTGTAAAACCGATCGCGATTGCTTAACTGGCTGCACTTGCAAACGGAACGGCTATTGTGGTTGACTCTTGGCTGTGTCGTCTCGTATCAGCTTCTACGGTGTCTTTGTTAGTGTGTTTTCTATTTTGTGATAATAATAACGCATGGTTGTTGTGTGTCTTTGTCATAGATCACTTTACACTCGTATCTATGAGTTAGTGTGATCATATCCGTGGCAATATGTGATATCCCATCTAGCTGTTTCGTGTAATATTATTATGTTTTCTATGTCACTATGGTCTTTCAGGATATCTTCAC

mRNA sequence

AAGAGAGAGAGAGAAAGCAAAGAAAGAGAGAAAAAAAAAATGGAGTGGAAAAAAGTTGCTTTAGTTGCAGTAATGGGGATGTTGGTTATGGCTACTTTTACAGAGTCGGTCGGTTTGGGGGTGGATGAGGAGATCATTCAATTAGTTTCGGACGGAGTGAATGAGTACTCACAAAATATTGGAAAGAAAAATGTAGGCTGCCCTCGAATCCTTATGAAGTGTAAAACCGATCGCGATTGCTTAACTGGCTGCACTTGCAAACGGAACGGCTATTGTGGTTGACTCTTGGCTGTGTCGTCTCGTATCAGCTTCTACGGTGTCTTTGTTAGTGTGTTTTCTATTTTGTGATAATAATAACGCATGGTTGTTGTGTGTCTTTGTCATAGATCACTTTACACTCGTATCTATGAGTTAGTGTGATCATATCCGTGGCAATATGTGATATCCCATCTAGCTGTTTCGTGTAATATTATTATGTTTTCTATGTCACTATGGTCTTTCAGGATATCTTCAC

Coding sequence (CDS)

ATGGAGTGGAAAAAAGTTGCTTTAGTTGCAGTAATGGGGATGTTGGTTATGGCTACTTTTACAGAGTCGGTCGGTTTGGGGGTGGATGAGGAGATCATTCAATTAGTTTCGGACGGAGTGAATGAGTACTCACAAAATATTGGAAAGAAAAATGTAGGCTGCCCTCGAATCCTTATGAAGTGTAAAACCGATCGCGATTGCTTAACTGGCTGCACTTGCAAACGGAACGGCTATTGTGGTTGA

Protein sequence

MEWKKVALVAVMGMLVMATFTESVGLGVDEEIIQLVSDGVNEYSQNIGKKNVGCPRILMKCKTDRDCLTGCTCKRNGYCG

Homology

[Close]

BLAST of IVF0025404 vs. ExPASy Swiss-Prot
Match: Q43667 (Trypsin inhibitor 1 OS=Trichosanthes kirilowii OX=3677 PE=3 SV=1)

HSP 1 Score: 81.3 bits (199), Expect = 5.6e-15
Identity = 39/65 (60.00%), Postives = 45/65 (69.23%), Query Frame = 0


Query: 17 MATFTESVGLGVD-EEIIQLVSDGVNEYSQNIGKKNVGCPRILMKCKTDRDCLTGCTCKR 76
          MA F ES   G   +E+IQLVSDGVNEYS+ + +  V CPRILM CK + DCL GC C  
Sbjct: 1  MAAFVESARAGAGADEVIQLVSDGVNEYSEKMMEGVVACPRILMPCKVNDDCLRGCKCLS 60

Query: 77 NGYCG 81
          NGYCG
Sbjct: 61 NGYCG 65

[Close]

BLAST of IVF0025404 vs. ExPASy Swiss-Prot
Match: P34950 (Trypsin inhibitor 5 OS=Luffa aegyptiaca OX=3670 PE=1 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 5.4e-10
Identity = 33/66 (50.00%), Postives = 42/66 (63.64%), Query Frame = 0


Query: 17 MATFTESVGLGVDEEIIQLVSDGVNEYSQNI--GKKNVGCPRILMKCKTDRDCLTGCTCK 76
          MA+  ES G+    E+I+L+SDG N+  + I  G+    CPRILM CKTD DC+  C C 
Sbjct: 1  MASVAESSGV---VEVIELISDGGNDLPRKIMSGRHGGICPRILMPCKTDDDCMLDCRCL 60

Query: 77 RNGYCG 81
           NGYCG
Sbjct: 61 SNGYCG 63

[Close]

BLAST of IVF0025404 vs. ExPASy Swiss-Prot
Match: P11968 (Trypsin inhibitor 2 OS=Bryonia dioica OX=3652 PE=1 SV=1)

HSP 1 Score: 57.8 bits (138), Expect = 6.7e-08
Identity = 21/28 (75.00%), Postives = 24/28 (85.71%), Query Frame = 0


Query: 53 GCPRILMKCKTDRDCLTGCTCKRNGYCG 81
          GCPRILM+CK D DCL GC C++NGYCG
Sbjct: 2  GCPRILMRCKRDSDCLAGCVCQKNGYCG 29

[Close]

BLAST of IVF0025404 vs. ExPASy Swiss-Prot
Match: P12071 (Trypsin inhibitor 2 OS=Ecballium elaterium OX=3679 PE=1 SV=2)

HSP 1 Score: 53.5 bits (127), Expect = 1.3e-06
Identity = 20/28 (71.43%), Postives = 22/28 (78.57%), Query Frame = 0


Query: 53 GCPRILMKCKTDRDCLTGCTCKRNGYCG 81
          GCPRILM+CK D DCL GC C  NG+CG
Sbjct: 1  GCPRILMRCKQDSDCLAGCVCGPNGFCG 28

[Close]

BLAST of IVF0025404 vs. ExPASy Swiss-Prot
Match: P17680 (Trypsin inhibitor 1 OS=Momordica repens OX=3675 PE=1 SV=1)

HSP 1 Score: 52.4 bits (124), Expect = 2.8e-06
Identity = 20/27 (74.07%), Postives = 21/27 (77.78%), Query Frame = 0


Query: 54 CPRILMKCKTDRDCLTGCTCKRNGYCG 81
          CPRILM+CK D DCL  C CKR GYCG
Sbjct: 3  CPRILMECKRDSDCLAQCVCKRQGYCG 29

[Close]

BLAST of IVF0025404 vs. ExPASy TrEMBL
Match: A0A0A0KY34 (Trypsin inhibitor 1 OS=Cucumis sativus OX=3659 GN=Csa_4G000810 PE=3 SV=1)

HSP 1 Score: 141.0 bits (354), Expect = 2.2e-30
Identity = 69/81 (85.19%), Postives = 72/81 (88.89%), Query Frame = 0


Query: 1  MEWKKVALVAVMGMLVMATFTESVGLGVDEEIIQLVSDGVNEYSQNIGKKNV-GCPRILM 60
          MEWKK+ALVA+MGML+MATFTESVGLGVDEEIIQLVSDGVNEYSQNIGK    GCPRILM
Sbjct: 1  MEWKKIALVAMMGMLLMATFTESVGLGVDEEIIQLVSDGVNEYSQNIGKDTAPGCPRILM 60

Query: 61 KCKTDRDCLTGCTCKRNGYCG 81
          KCKTD DC  GCTCK  GYCG
Sbjct: 61 KCKTDSDCYPGCTCKPMGYCG 81

[Close]

BLAST of IVF0025404 vs. ExPASy TrEMBL
Match: A0A5D3E0W0 (Trypsin inhibitor 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G00600 PE=3 SV=1)

HSP 1 Score: 137.1 bits (344), Expect = 3.2e-29
Identity = 67/80 (83.75%), Postives = 71/80 (88.75%), Query Frame = 0


Query: 1  MEWKKVALVAVMGMLVMATFTESVGLGVDEEIIQLVSDGVNEYSQNIGKKNVGCPRILMK 60
          MEWKK+ALVA+MGML+MATFTESVG GVDEEIIQLVSDGVNEYS      + GCPRILMK
Sbjct: 1  MEWKKIALVAMMGMLLMATFTESVGFGVDEEIIQLVSDGVNEYS-----GHPGCPRILMK 60

Query: 61 CKTDRDCLTGCTCKRNGYCG 81
          CKTDRDCL GCTCKRNGYCG
Sbjct: 61 CKTDRDCLAGCTCKRNGYCG 75

[Close]

BLAST of IVF0025404 vs. ExPASy TrEMBL
Match: A0A6J1JBL8 (trypsin inhibitor 1-like OS=Cucurbita maxima OX=3661 GN=LOC111482991 PE=3 SV=1)

HSP 1 Score: 92.4 bits (228), Expect = 9.0e-16
Identity = 43/80 (53.75%), Postives = 59/80 (73.75%), Query Frame = 0


Query: 1  MEWKKVALVAVMGMLVMATFTESVGLGVDEEIIQLVSDGVNEYSQNIGKKNVGCPRILMK 60
          MEWK+ AL+A++G+L+MA F ES  +    E+IQLVSDGVN++ + +     GCPRILM+
Sbjct: 1  MEWKRGALIAMVGLLLMAAFAESAAVH-GGEVIQLVSDGVNDFPRKMMNGVQGCPRILME 60

Query: 61 CKTDRDCLTGCTCKRNGYCG 81
          C+ D DCL GC C+ NG+CG
Sbjct: 61 CELDSDCLPGCICRPNGFCG 79

[Close]

BLAST of IVF0025404 vs. ExPASy TrEMBL
Match: A0A4P2SJP1 (Trypsin inhibitor I1 OS=Trichosanthes kirilowii OX=3677 PE=2 SV=1)

HSP 1 Score: 85.1 bits (209), Expect = 1.4e-13
Identity = 41/65 (63.08%), Postives = 46/65 (70.77%), Query Frame = 0


Query: 17 MATFTESVGLGVD-EEIIQLVSDGVNEYSQNIGKKNVGCPRILMKCKTDRDCLTGCTCKR 76
          MA F ES   G   +E+IQLVSDGVNEYS+ + K  VGCPRILM CK + DCL GC C  
Sbjct: 1  MAAFVESARAGAGADEVIQLVSDGVNEYSEKMMKGVVGCPRILMPCKVNDDCLRGCKCLS 60

Query: 77 NGYCG 81
          NGYCG
Sbjct: 61 NGYCG 65

[Close]

BLAST of IVF0025404 vs. ExPASy TrEMBL
Match: A0A4P2SLY2 (Trypsin inhibitor I2 OS=Trichosanthes kirilowii OX=3677 PE=2 SV=1)

HSP 1 Score: 83.2 bits (204), Expect = 5.5e-13
Identity = 39/65 (60.00%), Postives = 46/65 (70.77%), Query Frame = 0


Query: 17 MATFTESVGLGVD-EEIIQLVSDGVNEYSQNIGKKNVGCPRILMKCKTDRDCLTGCTCKR 76
          MA F ES   G   +E+IQLVSDGVNEYS+ + +  VGCPR+LM CK + DCL GC C  
Sbjct: 1  MAAFVESARAGAGADEVIQLVSDGVNEYSEKMMEGVVGCPRVLMPCKVNDDCLRGCKCLS 60

Query: 77 NGYCG 81
          NGYCG
Sbjct: 61 NGYCG 65

[Close]

BLAST of IVF0025404 vs. NCBI nr
Match: KGN52761.1 (hypothetical protein Csa_014657 [Cucumis sativus])

HSP 1 Score: 138 bits (348), Expect = 9.09e-41
Identity = 69/81 (85.19%), Postives = 72/81 (88.89%), Query Frame = 0


Query: 1  MEWKKVALVAVMGMLVMATFTESVGLGVDEEIIQLVSDGVNEYSQNIGKKNV-GCPRILM 60
          MEWKK+ALVA+MGML+MATFTESVGLGVDEEIIQLVSDGVNEYSQNIGK    GCPRILM
Sbjct: 1  MEWKKIALVAMMGMLLMATFTESVGLGVDEEIIQLVSDGVNEYSQNIGKDTAPGCPRILM 60

Query: 61 KCKTDRDCLTGCTCKRNGYCG 80
          KCKTD DC  GCTCK  GYCG
Sbjct: 61 KCKTDSDCYPGCTCKPMGYCG 81

[Close]

BLAST of IVF0025404 vs. NCBI nr
Match: KAA0044335.1 (Trypsin inhibitor 1 [Cucumis melo var. makuwa] >TYK29464.1 Trypsin inhibitor 1 [Cucumis melo var. makuwa])

HSP 1 Score: 135 bits (339), Expect = 1.81e-39
Identity = 67/80 (83.75%), Postives = 71/80 (88.75%), Query Frame = 0


Query: 1  MEWKKVALVAVMGMLVMATFTESVGLGVDEEIIQLVSDGVNEYSQNIGKKNVGCPRILMK 60
          MEWKK+ALVA+MGML+MATFTESVG GVDEEIIQLVSDGVNEYS +      GCPRILMK
Sbjct: 1  MEWKKIALVAMMGMLLMATFTESVGFGVDEEIIQLVSDGVNEYSGH-----PGCPRILMK 60

Query: 61 CKTDRDCLTGCTCKRNGYCG 80
          CKTDRDCL GCTCKRNGYCG
Sbjct: 61 CKTDRDCLAGCTCKRNGYCG 75

[Close]

BLAST of IVF0025404 vs. NCBI nr
Match: XP_022984819.1 (trypsin inhibitor 1-like [Cucurbita maxima])

HSP 1 Score: 90.5 bits (223), Expect = 9.87e-22
Identity = 43/80 (53.75%), Postives = 59/80 (73.75%), Query Frame = 0


Query: 1  MEWKKVALVAVMGMLVMATFTESVGLGVDEEIIQLVSDGVNEYSQNIGKKNVGCPRILMK 60
          MEWK+ AL+A++G+L+MA F ES  +    E+IQLVSDGVN++ + +     GCPRILM+
Sbjct: 1  MEWKRGALIAMVGLLLMAAFAESAAVH-GGEVIQLVSDGVNDFPRKMMNGVQGCPRILME 60

Query: 61 CKTDRDCLTGCTCKRNGYCG 80
          C+ D DCL GC C+ NG+CG
Sbjct: 61 CELDSDCLPGCICRPNGFCG 79

[Close]

BLAST of IVF0025404 vs. NCBI nr
Match: KAG6576915.1 (hypothetical protein SDJN03_24489, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 92.4 bits (228), Expect = 1.56e-21
Identity = 43/80 (53.75%), Postives = 60/80 (75.00%), Query Frame = 0


Query: 1   MEWKKVALVAVMGMLVMATFTESVGLGVDEEIIQLVSDGVNEYSQNIGKKNVGCPRILMK 60
           MEWK+ AL+A++G+L+MA F ES  +    E+IQLVSDGVN++ + +    +GCPRILM+
Sbjct: 83  MEWKRGALIAMVGLLLMAAFAESAAVH-GGEVIQLVSDGVNDFPRKMMNGALGCPRILME 142

Query: 61  CKTDRDCLTGCTCKRNGYCG 80
           C+ D DCL GC C+ NG+CG
Sbjct: 143 CEVDSDCLPGCICRPNGFCG 161

[Close]

BLAST of IVF0025404 vs. NCBI nr
Match: KAG7014942.1 (hypothetical protein SDJN02_22573, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 92.4 bits (228), Expect = 1.56e-21
Identity = 43/80 (53.75%), Postives = 60/80 (75.00%), Query Frame = 0


Query: 1   MEWKKVALVAVMGMLVMATFTESVGLGVDEEIIQLVSDGVNEYSQNIGKKNVGCPRILMK 60
           MEWK+ AL+A++G+L+MA F ES  +    E+IQLVSDGVN++ + +    +GCPRILM+
Sbjct: 83  MEWKRGALIAMVGLLLMAAFAESAAVH-GGEVIQLVSDGVNDFPRKMMNGALGCPRILME 142

Query: 61  CKTDRDCLTGCTCKRNGYCG 80
           C+ D DCL GC C+ NG+CG
Sbjct: 143 CEVDSDCLPGCICRPNGFCG 161

The following BLAST results are available for this feature:

BLAST of IVF0025404 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q43667	5.6e-15	60.00	Trypsin inhibitor 1 OS=Trichosanthes kirilowii OX=3677 PE=3 SV=1	[more]
P34950	5.4e-10	50.00	Trypsin inhibitor 5 OS=Luffa aegyptiaca OX=3670 PE=1 SV=1	[more]
P11968	6.7e-08	75.00	Trypsin inhibitor 2 OS=Bryonia dioica OX=3652 PE=1 SV=1	[more]
P12071	1.3e-06	71.43	Trypsin inhibitor 2 OS=Ecballium elaterium OX=3679 PE=1 SV=2	[more]
P17680	2.8e-06	74.07	Trypsin inhibitor 1 OS=Momordica repens OX=3675 PE=1 SV=1	[more]

BLAST of IVF0025404 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KY34	2.2e-30	85.19	Trypsin inhibitor 1 OS=Cucumis sativus OX=3659 GN=Csa_4G000810 PE=3 SV=1	[more]
A0A5D3E0W0	3.2e-29	83.75	Trypsin inhibitor 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G...	[more]
A0A6J1JBL8	9.0e-16	53.75	trypsin inhibitor 1-like OS=Cucurbita maxima OX=3661 GN=LOC111482991 PE=3 SV=1	[more]
A0A4P2SJP1	1.4e-13	63.08	Trypsin inhibitor I1 OS=Trichosanthes kirilowii OX=3677 PE=2 SV=1	[more]
A0A4P2SLY2	5.5e-13	60.00	Trypsin inhibitor I2 OS=Trichosanthes kirilowii OX=3677 PE=2 SV=1	[more]

BLAST of IVF0025404 vs. NCBI nr


Match Name	E-value	Identity	Description
KGN52761.1	9.09e-41	85.19	hypothetical protein Csa_014657 [Cucumis sativus]	[more]
KAA0044335.1	1.81e-39	83.75	Trypsin inhibitor 1 [Cucumis melo var. makuwa] >TYK29464.1 Trypsin inhibitor 1 [...	[more]
XP_022984819.1	9.87e-22	53.75	trypsin inhibitor 1-like [Cucurbita maxima]	[more]
KAG6576915.1	1.56e-21	53.75	hypothetical protein SDJN03_24489, partial [Cucurbita argyrosperma subsp. sorori...	[more]
KAG7014942.1	1.56e-21	53.75	hypothetical protein SDJN02_22573, partial [Cucurbita argyrosperma subsp. argyro...	[more]

BLAST of IVF0025404 vs. NCBI nr


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SMART	SM00286	PTI_1	coord: 52..80 e-value: 1.2E-9 score: 48.1
None	No IPR available	GENE3D	4.10.75.20		coord: 35..80 e-value: 4.2E-15 score: 57.3
IPR000737	Proteinase inhibitor I7, squash	PFAM	PF00299	Squash	coord: 54..80 e-value: 5.9E-14 score: 51.8
IPR000737	Proteinase inhibitor I7, squash	PROSITE	PS00286	SQUASH_INHIBITOR	coord: 54..73
IPR000737	Proteinase inhibitor I7, squash	CDD	cd00150	PlantTI	coord: 54..80 e-value: 1.056E-10 score: 49.2983
IPR011052	Proteinase/amylase inhibitor domain superfamily	SUPERFAMILY	57027	Plant inhibitors of proteinases and amylases	coord: 50..80

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
IVF0025404.2	IVF0025404.2	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
cellular_component	GO:0005576	extracellular region
molecular_function	GO:0004867	serine-type endopeptidase inhibitor activity
molecular_function	GO:0004866	endopeptidase inhibitor activity