IVF0025369 (gene) Melon (IVF77) v1

Overview
NameIVF0025369
Typegene
OrganismCucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
Description2-hydroxyacyl-CoA lyase
Locationchr07: 9029091 .. 9032406 (-)
RNA-Seq ExpressionIVF0025369
SyntenyIVF0025369
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTTCATATCTCAGATTTTTAGCCTTTTCGTTCTCTCCATTTCTCTCTACCGCAAAATCTCGAGCTCTAATGGGGGATTCAGCTGAAGCTCTTCAAAATGATCTCAGATTTCACGGTCCTATCGACGGCAACGTTCTTGCCGCCATGGCGCTGGCCCGCTGCGGCGTCGACCGCATGTTTGGCGTCGTCGGAATCCCTGTTACCTCTCTGGCCACTAGGGCAGTTTCTTTAGGAATTCGATTCATTGCCTTTCATAACGAACAATCGGCTGGCTATGCCGCTTCTGCTTATGGTTATCTAACGGGCCGCCCTGGTGTGCTGCTTACTGTTTCTGGCCCTGGTTGTGTTCATGGTCTTGCTGGGATTTCAAACGCCATGGTTAATGCTTGGCCTTTGGTGATGATCTCTGGTTCTTGTGATCAAAGAGATTTCGGCCGTGGTGATTTTCAGGAGCTTGATCAGGTGGAGGCTGTGAAGCCCTTTTCGAAAATTTCCGTGAAGGCCACGGATATTTCCGAGATTCCGAATTGCGTTGCTCGAGTTCTTAATTCGGCTGTTTCTGGTCGGCCTGGTGGGTGCTATTTTGATCTTCCATCGGATGTCTTGCATCAGACTATTTCCGAGTCTGAGGCGGAGAGATTATTGGCCGCAGCGGAGGAATTTGCAAATCGCGAAGTGATTCCTAGAGTTCCGAACTCGCAGATTGAGGAAGCTATCTCATTGTTGAAACATGCGGAACGGCCACTGATTGTGTTTGGTAAAGGGGCAGCCATTGCTCGTGCTGAGGGGCCGTTGAAGAAGCTTGTGGAGACTACTGGAATTCCATTTCTTCCTACTCCAATGGGTAAAGGATTATTGCCAGATACACATGAGCTTGCGGCTACCGCCGCTAGGTCATTGGCGATTGGTAAGTGCGATGTTGCGCTTGTGGTTGGGGCAAGGCTTAATTGGTTGTTGCATTTTGGCGAGCCGCCTAAATGGTCCAAGGACGTGAAGTTCATTTTGGTTGATATTTGCAAGGAGGAAGTTGAATTAAGAAAGCCCCATTTGGGTTTGATTGGCGATGCAAAAGAGGTACTGGAATCCATAAACAAGGAGATCAAGGATGACCCATTTTGTCTTGGAAAATCTCATCCATGGGTTGGAGCAATTTCGCAGAAGGCAAAGGACAATGTGGCGAAAATGGAAGTTCAGCTGGCGAAGGATGTTGTGCCATTCAATTTCTTGACGCCAATGAGAATCATTCGAGATGCTATATTGGCTGTTGGTAGCCCTGCACCCATATTGGTCTCTGAAGGGGCCAATACAATGGATGTGGGTCGTTCAGTTTTGGTTCAAACTGAGCCAAGGACCAGGCTGGATGCAGGAACATGGGGGACCATGGGGGTTGGCTTGGGCTATTGCATTGCAGCTGCGGTTGCTTCGCCTGATCGTTTGGTAGTTGCAGTGGAAGGCGATTCTGGATTTGGGTTCAGTGCCATGGAAGTTGAGGTATTCAGTCTCCCCTTTGCACTTTTCTTCCATCAACTTGTTTCATGTTGTATGATGATTATTTGATTCTTAACTCATTGAAGAGTCTTGCATATATTTTTAGAAGGGAATAGACTTGTCATCGAAGAAATGAAAAAAAGACTAATGTTCAAAATAGGATGAGGCAAAAAGGAACTAAAACTTAGAATCATATGATCAAAGATGCATTTGGATATCTCGGCTAGGTTCACACATCATAACACCCCAAGTACAAATATCTACTTAGCAAACTCAAAATTTCGCAAATACACGATTACATGGGGAAAATAAAAGCATTCCAATTAAGACAAATATCCTGAATAGAGTAGTTCGCTAAAAAACTTGGATTGACGAATATCCTGTAGAGCGTCCTGCATATTAAAACATTGTGTAGTTCGTTCCAATGTTGGCTTATCATTGTTACTTTCTGGTTTGGAAATGTTTATTGGTTGGTTACTATGTTTCATTTCAGCTGTCCCTAGGTAAACATGAATGACATAGAAAAAAACATGTAGACACTGGTGATCAGTAGCTCTAGGATTATTCTTGGTCATGTTTTGTAGGATGTCTTTAAACGGGTTGCTTCCTTGTTTTGATGGTTGAGTTTGTAGATGTCATTCTCATTTCATTTTAGGTACGATATGTTGTTTGTTTCACAGGGGCTTACTAGATTTCCCTTATTTTTTGTACTTAGATCTTTGTGTATTTATTTTCTTTGTTCATTTTAACACCATGATTGTTTTGTCAAATTACATGTGTCACAAGGAGTCTCACGGATCAACTGGGCCTAGAATATTGGACTCGTAATGTTTAATGTCCTAAGTAGGCACCGTAGCACTTCATACAAATTTCCATTAGTCAAATCTGAAATTTCGATTATTGTCGTAACTCGCAAGTGATATAAGTAAGTTCTGGAGTTTTAGAACTCCATTTAAGTTCTAGAAGTTGTACATCTTACTTTGACTTGGGTTCTACTGTAAATGGTGTTTGATTTTCCCTTGTGTTCTTTCTAAAGAGCTCTTTATAATCTTTAATTGGCCAGTTTTGCTCCTCATAATCTAAGAACATTTATTCAAATGAGATCTCAGAAACAAACCGATGGTTTCTTTTTCTTTCCTATTTGCAAAATGTCTCTATGTGTCTTTCCAATCCAACCAGACAAGTTTTGTGCTTGTCTACTTCCAAGTGTAGAAGCTTTTCCTTTTAGGTGGGAGATCTCTTCTCATCTGATGTACATTTTTCTGGTTTTCTACAGACACTGGTGCGGTATGAGTTGCCAGTGGTTGTGATAGTTTTCAACAACAGTGGTGTATATGGTGGCGACCGGAGAACACCACAAGAAATTACTGGACCCTATAAGCACGATCCTGCTCCGACCTCCTTTGTCCCATCAGCTAGTTATGACAAAATGATTGAGGCATTTGGTGGTAAAGGATACTATGTCGAATCACCCGAGGAACTAAAATCTGCACTAGCTGAGTCCTTTTCTGCTCGGAAACCAGCAGTTGTAAACGTCAAGATTGATCCGTATGCCGGTGCAGAAAGTGGAAGGCTGCAACACAAGAACTGAAGTTAAGTCCTTACTTCTGTGTTGTATTTCTTTGCTTAAGACTCTGTGCAAATGAATTAGGTTTGCAGCCGAGTTTCAAGCTAACAAGCTTAGTTTTCACACTCTCTTCTCTTTTATTTCTTTCTTTTTGTTAGCCTATAATCATGTAAATTATGGCGTTCGAATAAAATTGAATGTAACTTGTAAAGCCAGCAGTTGGATAAATGATTTAATAGAACTTCAAGCTTAAATCCATCA

mRNA sequence

CTTTCATATCTCAGATTTTTAGCCTTTTCGTTCTCTCCATTTCTCTCTACCGCAAAATCTCGAGCTCTAATGGGGGATTCAGCTGAAGCTCTTCAAAATGATCTCAGATTTCACGGTCCTATCGACGGCAACGTTCTTGCCGCCATGGCGCTGGCCCGCTGCGGCGTCGACCGCATGTTTGGCGTCGTCGGAATCCCTGTTACCTCTCTGGCCACTAGGGCAGTTTCTTTAGGAATTCGATTCATTGCCTTTCATAACGAACAATCGGCTGGCTATGCCGCTTCTGCTTATGGTTATCTAACGGGCCGCCCTGGTGTGCTGCTTACTGTTTCTGGCCCTGGTTGTGTTCATGGTCTTGCTGGGATTTCAAACGCCATGGTTAATGCTTGGCCTTTGGTGATGATCTCTGGTTCTTGTGATCAAAGAGATTTCGGCCGTGGTGATTTTCAGGAGCTTGATCAGGTGGAGGCTGTGAAGCCCTTTTCGAAAATTTCCGTGAAGGCCACGGATATTTCCGAGATTCCGAATTGCGTTGCTCGAGTTCTTAATTCGGCTGTTTCTGGTCGGCCTGGTGGGTGCTATTTTGATCTTCCATCGGATGTCTTGCATCAGACTATTTCCGAGTCTGAGGCGGAGAGATTATTGGCCGCAGCGGAGGAATTTGCAAATCGCGAAGTGATTCCTAGAGTTCCGAACTCGCAGATTGAGGAAGCTATCTCATTGTTGAAACATGCGGAACGGCCACTGATTGTGTTTGGTAAAGGGGCAGCCATTGCTCGTGCTGAGGGGCCGTTGAAGAAGCTTGTGGAGACTACTGGAATTCCATTTCTTCCTACTCCAATGGGTAAAGGATTATTGCCAGATACACATGAGCTTGCGGCTACCGCCGCTAGGTCATTGGCGATTGGTAAGTGCGATGTTGCGCTTGTGGTTGGGGCAAGGCTTAATTGGTTGTTGCATTTTGGCGAGCCGCCTAAATGGTCCAAGGACGTGAAGTTCATTTTGGTTGATATTTGCAAGGAGGAAGTTGAATTAAGAAAGCCCCATTTGGGTTTGATTGGCGATGCAAAAGAGGTACTGGAATCCATAAACAAGGAGATCAAGGATGACCCATTTTGTCTTGGAAAATCTCATCCATGGGTTGGAGCAATTTCGCAGAAGGCAAAGGACAATGTGGCGAAAATGGAAGTTCAGCTGGCGAAGGATGTTGTGCCATTCAATTTCTTGACGCCAATGAGAATCATTCGAGATGCTATATTGGCTGTTGGTAGCCCTGCACCCATATTGGTCTCTGAAGGGGCCAATACAATGGATGTGGGTCGTTCAGTTTTGGTTCAAACTGAGCCAAGGACCAGGCTGGATGCAGGAACATGGGGGACCATGGGGGTTGGCTTGGGCTATTGCATTGCAGCTGCGGTTGCTTCGCCTGATCGTTTGGTAGTTGCAGTGGAAGGCGATTCTGGATTTGGGTTCAGTGCCATGGAAGTTGAGACACTGGTGCGGTATGAGTTGCCAGTGGTTGTGATAGTTTTCAACAACAGTGGTGTATATGGTGGCGACCGGAGAACACCACAAGAAATTACTGGACCCTATAAGCACGATCCTGCTCCGACCTCCTTTGTCCCATCAGCTAGTTATGACAAAATGATTGAGGCATTTGGTGGTAAAGGATACTATGTCGAATCACCCGAGGAACTAAAATCTGCACTAGCTGAGTCCTTTTCTGCTCGGAAACCAGCAGTTGTAAACGTCAAGATTGATCCGTATGCCGGTGCAGAAAGTGGAAGGCTGCAACACAAGAACTGAAGTTAAGTCCTTACTTCTGTGTTGTATTTCTTTGCTTAAGACTCTGTGCAAATGAATTAGGTTTGCAGCCGAGTTTCAAGCTAACAAGCTTAGTTTTCACACTCTCTTCTCTTTTATTTCTTTCTTTTTGTTAGCCTATAATCATGTAAATTATGGCGTTCGAATAAAATTGAATGTAACTTGTAAAGCCAGCAGTTGGATAAATGATTTAATAGAACTTCAAGCTTAAATCCATCA

Coding sequence (CDS)

ATGGGGGATTCAGCTGAAGCTCTTCAAAATGATCTCAGATTTCACGGTCCTATCGACGGCAACGTTCTTGCCGCCATGGCGCTGGCCCGCTGCGGCGTCGACCGCATGTTTGGCGTCGTCGGAATCCCTGTTACCTCTCTGGCCACTAGGGCAGTTTCTTTAGGAATTCGATTCATTGCCTTTCATAACGAACAATCGGCTGGCTATGCCGCTTCTGCTTATGGTTATCTAACGGGCCGCCCTGGTGTGCTGCTTACTGTTTCTGGCCCTGGTTGTGTTCATGGTCTTGCTGGGATTTCAAACGCCATGGTTAATGCTTGGCCTTTGGTGATGATCTCTGGTTCTTGTGATCAAAGAGATTTCGGCCGTGGTGATTTTCAGGAGCTTGATCAGGTGGAGGCTGTGAAGCCCTTTTCGAAAATTTCCGTGAAGGCCACGGATATTTCCGAGATTCCGAATTGCGTTGCTCGAGTTCTTAATTCGGCTGTTTCTGGTCGGCCTGGTGGGTGCTATTTTGATCTTCCATCGGATGTCTTGCATCAGACTATTTCCGAGTCTGAGGCGGAGAGATTATTGGCCGCAGCGGAGGAATTTGCAAATCGCGAAGTGATTCCTAGAGTTCCGAACTCGCAGATTGAGGAAGCTATCTCATTGTTGAAACATGCGGAACGGCCACTGATTGTGTTTGGTAAAGGGGCAGCCATTGCTCGTGCTGAGGGGCCGTTGAAGAAGCTTGTGGAGACTACTGGAATTCCATTTCTTCCTACTCCAATGGGTAAAGGATTATTGCCAGATACACATGAGCTTGCGGCTACCGCCGCTAGGTCATTGGCGATTGGTAAGTGCGATGTTGCGCTTGTGGTTGGGGCAAGGCTTAATTGGTTGTTGCATTTTGGCGAGCCGCCTAAATGGTCCAAGGACGTGAAGTTCATTTTGGTTGATATTTGCAAGGAGGAAGTTGAATTAAGAAAGCCCCATTTGGGTTTGATTGGCGATGCAAAAGAGGTACTGGAATCCATAAACAAGGAGATCAAGGATGACCCATTTTGTCTTGGAAAATCTCATCCATGGGTTGGAGCAATTTCGCAGAAGGCAAAGGACAATGTGGCGAAAATGGAAGTTCAGCTGGCGAAGGATGTTGTGCCATTCAATTTCTTGACGCCAATGAGAATCATTCGAGATGCTATATTGGCTGTTGGTAGCCCTGCACCCATATTGGTCTCTGAAGGGGCCAATACAATGGATGTGGGTCGTTCAGTTTTGGTTCAAACTGAGCCAAGGACCAGGCTGGATGCAGGAACATGGGGGACCATGGGGGTTGGCTTGGGCTATTGCATTGCAGCTGCGGTTGCTTCGCCTGATCGTTTGGTAGTTGCAGTGGAAGGCGATTCTGGATTTGGGTTCAGTGCCATGGAAGTTGAGACACTGGTGCGGTATGAGTTGCCAGTGGTTGTGATAGTTTTCAACAACAGTGGTGTATATGGTGGCGACCGGAGAACACCACAAGAAATTACTGGACCCTATAAGCACGATCCTGCTCCGACCTCCTTTGTCCCATCAGCTAGTTATGACAAAATGATTGAGGCATTTGGTGGTAAAGGATACTATGTCGAATCACCCGAGGAACTAAAATCTGCACTAGCTGAGTCCTTTTCTGCTCGGAAACCAGCAGTTGTAAACGTCAAGATTGATCCGTATGCCGGTGCAGAAAGTGGAAGGCTGCAACACAAGAACTGA

Protein sequence

MGDSAEALQNDLRFHGPIDGNVLAAMALARCGVDRMFGVVGIPVTSLATRAVSLGIRFIAFHNEQSAGYAASAYGYLTGRPGVLLTVSGPGCVHGLAGISNAMVNAWPLVMISGSCDQRDFGRGDFQELDQVEAVKPFSKISVKATDISEIPNCVARVLNSAVSGRPGGCYFDLPSDVLHQTISESEAERLLAAAEEFANREVIPRVPNSQIEEAISLLKHAERPLIVFGKGAAIARAEGPLKKLVETTGIPFLPTPMGKGLLPDTHELAATAARSLAIGKCDVALVVGARLNWLLHFGEPPKWSKDVKFILVDICKEEVELRKPHLGLIGDAKEVLESINKEIKDDPFCLGKSHPWVGAISQKAKDNVAKMEVQLAKDVVPFNFLTPMRIIRDAILAVGSPAPILVSEGANTMDVGRSVLVQTEPRTRLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYELPVVVIVFNNSGVYGGDRRTPQEITGPYKHDPAPTSFVPSASYDKMIEAFGGKGYYVESPEELKSALAESFSARKPAVVNVKIDPYAGAESGRLQHKN
Homology
BLAST of IVF0025369 vs. ExPASy Swiss-Prot
Match: Q9LF46 (2-hydroxyacyl-CoA lyase OS=Arabidopsis thaliana OX=3702 GN=HACL PE=1 SV=1)

HSP 1 Score: 915.6 bits (2365), Expect = 2.8e-265
Identity = 450/561 (80.21%), Postives = 501/561 (89.30%), Query Frame = 0

Query: 18  IDGNVLAAMALARCGVDRMFGVVGIPVTSLATRAVSLGIRFIAFHNEQSAGYAASAYGYL 77
           IDGNVL A +L+  GV  MFGVVGIPVTSLA+RA++LGIRFIAFHNEQSAGYAASAYGYL
Sbjct: 12  IDGNVLVAKSLSHLGVTHMFGVVGIPVTSLASRAMALGIRFIAFHNEQSAGYAASAYGYL 71

Query: 78  TGRPGVLLTVSGPGCVHGLAGISNAMVNAWPLVMISGSCDQRDFGRGDFQELDQVEAVKP 137
           TG+PG+LLTVSGPGCVHGLAG+SNA VN WP+VMISGSCDQRD GRGDFQELDQ+EAVK 
Sbjct: 72  TGKPGILLTVSGPGCVHGLAGLSNAWVNTWPMVMISGSCDQRDVGRGDFQELDQIEAVKA 131

Query: 138 FSKISVKATDISEIPNCVARVLNSAVSGRPGGCYFDLPSDVLHQTISESEAERLLAAAEE 197
           FSK+S KA D+ EIP+CV+RVL+ AVSGRPGGCY D+P+DVL Q ISESEA++L+   E 
Sbjct: 132 FSKLSEKAKDVREIPDCVSRVLDRAVSGRPGGCYLDIPTDVLRQKISESEADKLVDEVER 191

Query: 198 FANREVIPRVPNSQIEEAISLLKHAERPLIVFGKGAAIARAEGPLKKLVETTGIPFLPTP 257
               E I     S+IE A+SLL+ AERPLIVFGKGAA +RAE  LKKLVE TGIPFLPTP
Sbjct: 192 SRKEEPIRGSLRSEIESAVSLLRKAERPLIVFGKGAAYSRAEDELKKLVEITGIPFLPTP 251

Query: 258 MGKGLLPDTHELAATAARSLAIGKCDVALVVGARLNWLLHFGEPPKWSKDVKFILVDICK 317
           MGKGLLPDTHE +ATAARSLAIGKCDVALVVGARLNWLLHFGE PKW KDVKFILVD+ +
Sbjct: 252 MGKGLLPDTHEFSATAARSLAIGKCDVALVVGARLNWLLHFGESPKWDKDVKFILVDVSE 311

Query: 318 EEVELRKPHLGLIGDAKEVLESINKEIKDDPFCLGKSHPWVGAISQKAKDNVAKMEVQLA 377
           EE+ELRKPHLG++GDAK V+  +N+EIKDDPFCLGKS+ WV +IS+KAK+N  KME+QLA
Sbjct: 312 EEIELRKPHLGIVGDAKTVIGLLNREIKDDPFCLGKSNSWVESISKKAKENGEKMEIQLA 371

Query: 378 KDVVPFNFLTPMRIIRDAILAVGSPAPILVSEGANTMDVGRSVLVQTEPRTRLDAGTWGT 437
           KDVVPFNFLTPMRIIRDAILAV  P+P++VSEGANTMDVGRSVLVQ EPRTRLDAGTWGT
Sbjct: 372 KDVVPFNFLTPMRIIRDAILAVEGPSPVVVSEGANTMDVGRSVLVQKEPRTRLDAGTWGT 431

Query: 438 MGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYELPVVVIVFNNSGVYGGD 497
           MGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRY L VV+IVFNN GVYGGD
Sbjct: 432 MGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYNLAVVIIVFNNGGVYGGD 491

Query: 498 RRTPQEITGPYKHDPAPTSFVPSASYDKMIEAFGGKGYYVESPEELKSALAESFSARKPA 557
           RR P+EI+GP+K DPAPTSFVP+A Y K+IEAFGGKGY VE+P+ELKSALAESF+ARKPA
Sbjct: 492 RRGPEEISGPHKEDPAPTSFVPNAGYHKLIEAFGGKGYIVETPDELKSALAESFAARKPA 551

Query: 558 VVNVKIDPYAGAESGRLQHKN 579
           VVNV IDP+AGAESGRLQHKN
Sbjct: 552 VVNVIIDPFAGAESGRLQHKN 572

BLAST of IVF0025369 vs. ExPASy Swiss-Prot
Match: Q0JMH0 (2-hydroxyacyl-CoA lyase OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0505400 PE=3 SV=3)

HSP 1 Score: 883.2 bits (2281), Expect = 1.5e-255
Identity = 430/565 (76.11%), Postives = 495/565 (87.61%), Query Frame = 0

Query: 18  IDGNVLAAMALARCGVDRMFGVVGIPVTSLATRAVSLGIRFIAFHNEQSAGYAASAYGYL 77
           +DG+ LA  ALA  G   MFGVVGIPVTSLA+RA + G+RF+AF NEQSAGYAA+AYG+L
Sbjct: 13  VDGSALAGRALAAAGARHMFGVVGIPVTSLASRAAAAGVRFLAFRNEQSAGYAAAAYGFL 72

Query: 78  TGRPGVLLTVSGPGCVHGLAGISNAMVNAWPLVMISGSCDQRDFGRGDFQELDQVEAVKP 137
           TG PG+LLTVSGPGCVHGLAG+S+A  NAWPL+M+SGSC Q D GRGDFQELDQ+ A KP
Sbjct: 73  TGSPGLLLTVSGPGCVHGLAGLSHATANAWPLLMVSGSCSQPDAGRGDFQELDQIAATKP 132

Query: 138 FSKISVKATDISEIPNCVARVLNSAVSGRPGGCYFDLPSDVLHQTISESEAERLL-AAAE 197
           F KI+VKAT I++IP  V + L + VSGRPGGCY D+PSDVLHQT++ESEA  L+ AAA 
Sbjct: 133 FIKIAVKATTIADIPRLVFQALAATVSGRPGGCYLDIPSDVLHQTLTESEAAALIDAAAA 192

Query: 198 EFANREVIP---RVPNSQIEEAISLLKHAERPLIVFGKGAAIARAEGPLKKLVETTGIPF 257
           + A  +  P   +  +  IE+A  LL+ AERPL+VFGKGAA +RAE  + KLV+TTGIPF
Sbjct: 193 DSAKSDSSPPKHKSLDEGIEKAAELLRRAERPLVVFGKGAAYSRAEDAIWKLVDTTGIPF 252

Query: 258 LPTPMGKGLLPDTHELAATAARSLAIGKCDVALVVGARLNWLLHFGEPPKWSKDVKFILV 317
           LPTPMGKG++PDTH L+ATAARSLAIG+CDVALVVGARLNWLLHFGEPPKWSKDVKFILV
Sbjct: 253 LPTPMGKGVVPDTHPLSATAARSLAIGQCDVALVVGARLNWLLHFGEPPKWSKDVKFILV 312

Query: 318 DICKEEVELRKPHLGLIGDAKEVLESINKEIKDDPFCLGKSHPWVGAISQKAKDNVAKME 377
           D+C+EE+ELRKPH+G++GDAK V+E IN+EIKD PFCL  SHPWV AI++KA+DNV KME
Sbjct: 313 DVCEEEIELRKPHVGIVGDAKRVVELINREIKDQPFCLAPSHPWVEAITKKARDNVLKME 372

Query: 378 VQLAKDVVPFNFLTPMRIIRDAILAVGSPAPILVSEGANTMDVGRSVLVQTEPRTRLDAG 437
            QLAKDVVPFNFLTP+RIIRDAILA G+PAP++VSEGANTMDVGR+VLVQ EPRTRLDAG
Sbjct: 373 AQLAKDVVPFNFLTPLRIIRDAILAEGNPAPVVVSEGANTMDVGRAVLVQNEPRTRLDAG 432

Query: 438 TWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYELPVVVIVFNNSGV 497
           TWGTMGVGLG+C+AAAVA PDRLVVAVEGDSGFGFSAMEVETLVRY+LPVVVIVFNN+GV
Sbjct: 433 TWGTMGVGLGFCVAAAVAEPDRLVVAVEGDSGFGFSAMEVETLVRYQLPVVVIVFNNNGV 492

Query: 498 YGGDRRTPQEITGPYKHDPAPTSFVPSASYDKMIEAFGGKGYYVESPEELKSALAESFSA 557
           YGGDRR+P EITGPYK DPAPTSFVP+A Y KM+EAFGGKGY VE+P+ELKSAL+ESF A
Sbjct: 493 YGGDRRSPDEITGPYKDDPAPTSFVPAAGYHKMMEAFGGKGYLVETPDELKSALSESFRA 552

Query: 558 RKPAVVNVKIDPYAGAESGRLQHKN 579
           RKPAV+NV IDPYAGAESGR+QHKN
Sbjct: 553 RKPAVINVIIDPYAGAESGRMQHKN 577

BLAST of IVF0025369 vs. ExPASy Swiss-Prot
Match: Q8CHM7 (2-hydroxyacyl-CoA lyase 1 OS=Rattus norvegicus OX=10116 GN=Hacl1 PE=1 SV=1)

HSP 1 Score: 461.1 bits (1185), Expect = 1.9e-128
Identity = 251/575 (43.65%), Postives = 359/575 (62.43%), Query Frame = 0

Query: 1   MGDSAEALQNDLRFHGPIDGNVLAAMALARCGVDRMFGVVGIPVTSLATRAVSLGIRFIA 60
           M +S  A  +D R    + G  + A AL    V+ MFGVVGIPVT +A  A  LGI++I 
Sbjct: 1   MSESNSAEGSD-RSEEQVSGAKVIAQALKTQDVEYMFGVVGIPVTEIALAAQELGIKYIG 60

Query: 61  FHNEQSAGYAASAYGYLTGRPGVLLTVSGPGCVHGLAGISNAMVNAWPLVMISGSCDQRD 120
             NEQ+A YAASA GYLTGRPGV L VSGPG +H L G++NA +N WPL++I GS ++  
Sbjct: 61  MRNEQAACYAASAVGYLTGRPGVCLVVSGPGLIHALGGMANANMNCWPLIVIGGSSERNQ 120

Query: 121 FGRGDFQELDQVEAVKPFSKISVKATDISEIPNCVARVLNSAVSGRPGGCYFDLPSDVLH 180
              G FQE  QVEA + +SK S + + I  IP  + + + S++ GRPG CY D+P+D++ 
Sbjct: 121 EAMGAFQEFPQVEACRLYSKFSARPSSIQHIPFVIEKAVRSSIYGRPGACYIDVPADLV- 180

Query: 181 QTISESEAERLLAAAEEFANREVIPRVPNSQIEEAISLLKHAERPLIVFGKGAAIARAEG 240
            T+ ES         +E      +     S +  A S+L+ A++PL++ GKGAA + AE 
Sbjct: 181 -TLQESTTS---IKYKECCMPPPLSLAETSAVRAAASVLRSAKQPLLIIGKGAAYSHAED 240

Query: 241 PLKKLVETTGIPFLPTPMGKGLLPDTHELAATAARSLAIGKCDVALVVGARLNWLLHFGE 300
            ++KLVE   +PFLPTPMGKG++PD H     AARS A+   DV ++ GARLNW+LHFG 
Sbjct: 241 SIRKLVELCNLPFLPTPMGKGVVPDNHPNCVGAARSRALQFADVIVLFGARLNWILHFGL 300

Query: 301 PPKWSKDVKFILVDICKEEVELR-KPHLGLIGDAKEVLESINKEIKDDPFCLGKSHPWVG 360
           PP++  DVKFI +DIC EE+    +P + L+GD   V + + ++   +P+       W  
Sbjct: 301 PPRYQADVKFIQIDICAEELGNNVRPSVTLLGDVNAVSKQLLEQFVKNPWQYPTDSKWWE 360

Query: 361 AISQKAKDNVAKMEVQLAKDVVPFNFLTPMRIIRDAILAVGSPAPILVSEGANTMDVGRS 420
            + +K K+N A  +   +K  +P N+ T    +++ +         +VSEGANTMD+GR+
Sbjct: 361 TLREKRKNNEAVSKELASKKSLPMNYYTVFYHVQEQL----PRNCFIVSEGANTMDIGRT 420

Query: 421 VLVQTEPRTRLDAGTWGTMGVGLGYCIAAAVA----SPDRLVVAVEGDSGFGFSAMEVET 480
           VL    PR RLDAG++GTMGVGLG+ IAAAV     SP + V+ VEGDS FGFS MEVET
Sbjct: 421 VLQNYLPRHRLDAGSFGTMGVGLGFAIAAAVVAKERSPGQRVICVEGDSAFGFSGMEVET 480

Query: 481 LVRYELPVVVIVFNNSGVYGG-DRRTPQEI---TGPYKHDPAPTSFVPSASYDKMIEAFG 540
           + RY LP++++V NN+G+Y G D  T  +I    G     P P   +P++ Y++++ AFG
Sbjct: 481 ICRYNLPIIILVVNNNGIYQGFDADTWGKILNFQGTATTIP-PMCLLPNSHYEQVMTAFG 540

Query: 541 GKGYYVESPEELKSALAESF-SARKPAVVNVKIDP 566
           GKGY+V++PEEL+ +L ++     KP ++N+ I+P
Sbjct: 541 GKGYFVQTPEELQDSLRQALKDTSKPCLINIMIEP 564

BLAST of IVF0025369 vs. ExPASy Swiss-Prot
Match: Q9UJ83 (2-hydroxyacyl-CoA lyase 1 OS=Homo sapiens OX=9606 GN=HACL1 PE=1 SV=2)

HSP 1 Score: 457.6 bits (1176), Expect = 2.1e-127
Identity = 244/560 (43.57%), Postives = 356/560 (63.57%), Query Frame = 0

Query: 18  IDGNVLAAMALARCGVDRMFGVVGIPVTSLATRAVSLGIRFIAFHNEQSAGYAASAYGYL 77
           + G  + A AL    V+ +FG+VGIPVT +A  A  LGI++I   NEQ+A YAASA GYL
Sbjct: 14  VSGAKVIAQALKTQDVEYIFGIVGIPVTEIAIAAQQLGIKYIGMRNEQAACYAASAIGYL 73

Query: 78  TGRPGVLLTVSGPGCVHGLAGISNAMVNAWPLVMISGSCDQRDFGRGDFQELDQVEAVKP 137
           T RPGV L VSGPG +H L G++NA +N WPL++I GS ++     G FQE  QVEA + 
Sbjct: 74  TSRPGVCLVVSGPGLIHALGGMANANMNCWPLLVIGGSSERNQETMGAFQEFPQVEACRL 133

Query: 138 FSKISVKATDISEIPNCVARVLNSAVSGRPGGCYFDLPSDVLHQTISESEAERLLAAAEE 197
           ++K S + + I  IP  + + + S++ GRPG CY D+P+D ++  ++ +  + +     E
Sbjct: 134 YTKFSARPSSIEAIPFVIEKAVRSSIYGRPGACYVDIPADFVNLQVNVNSIKYM-----E 193

Query: 198 FANREVIPRVPNSQIEEAISLLKHAERPLIVFGKGAAIARAEGPLKKLVETTGIPFLPTP 257
                 I     S +  A S++++A++PL++ GKGAA A AE  +KKLVE   +PFLPTP
Sbjct: 194 RCMSPPISMAETSAVCTAASVIRNAKQPLLIIGKGAAYAHAEESIKKLVEQYKLPFLPTP 253

Query: 258 MGKGLLPDTHELAATAARSLAIGKCDVALVVGARLNWLLHFGEPPKWSKDVKFILVDICK 317
           MGKG++PD H     AARS A+   DV ++ GARLNW+LHFG PP++  DVKFI VDIC 
Sbjct: 254 MGKGVVPDNHPYCVGAARSRALQFADVIVLFGARLNWILHFGLPPRYQPDVKFIQVDICA 313

Query: 318 EEVELR-KPHLGLIGDAKEVLESINKEIKDDPFCLGKSHPWVGAISQKAKDNVAKMEVQL 377
           EE+    KP + L+G+   V + + +E+   P+       W   + +K K N A  +   
Sbjct: 314 EELGNNVKPAVTLLGNIHAVTKQLLEELDKTPWQYPPESKWWKTLREKMKSNEAASKELA 373

Query: 378 AKDVVPFNFLTPMRIIRDAILAVGSPAPILVSEGANTMDVGRSVLVQTEPRTRLDAGTWG 437
           +K  +P N+ T    +++ +         +VSEGANTMD+GR+VL    PR RLDAGT+G
Sbjct: 374 SKKSLPMNYYTVFYHVQEQL----PRDCFVVSEGANTMDIGRTVLQNYLPRHRLDAGTFG 433

Query: 438 TMGVGLGYCIAAAVA----SPDRLVVAVEGDSGFGFSAMEVETLVRYELPVVVIVFNNSG 497
           TMGVGLG+ IAAAV     SP + ++ VEGDS FGFS MEVET+ RY LP++++V NN+G
Sbjct: 434 TMGVGLGFAIAAAVVAKDRSPGQWIICVEGDSAFGFSGMEVETICRYNLPIILLVVNNNG 493

Query: 498 VYGG-DRRTPQEITGPYKHDPA---PTSFVPSASYDKMIEAFGGKGYYVESPEELKSALA 557
           +Y G D  T +E+   ++   A   P   +P++ Y++++ AFGGKGY+V++PEEL+ +L 
Sbjct: 494 IYQGFDTDTWKEML-KFQDATAVVPPMCLLPNSHYEQVMTAFGGKGYFVQTPEELQKSLR 553

Query: 558 ESFS-ARKPAVVNVKIDPYA 568
           +S +   KP+++N+ I+P A
Sbjct: 554 QSLADTTKPSLINIMIEPQA 563

BLAST of IVF0025369 vs. ExPASy Swiss-Prot
Match: P0AFI1 (Oxalyl-CoA decarboxylase OS=Escherichia coli O157:H7 OX=83334 GN=oxc PE=3 SV=1)

HSP 1 Score: 457.2 bits (1175), Expect = 2.7e-127
Identity = 245/563 (43.52%), Postives = 358/563 (63.59%), Query Frame = 0

Query: 19  DGNVLAAMALARCGVDRMFGVVGIPVTSLATRAVSLGIRFIAFHNEQSAGYAASAYGYLT 78
           DG  +   AL +  +D ++GVVGIPVT +A  A + GIR+I F +EQSAGYAA+A G+LT
Sbjct: 9   DGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAEGIRYIGFRHEQSAGYAAAASGFLT 68

Query: 79  GRPGVLLTVSGPGCVHGLAGISNAMVNAWPLVMISGSCDQR--DFGRGDFQELDQVEAVK 138
            +PG+ LTVS PG ++GL  ++NA VN +P++MISGS D+   D  +GD++ELDQ+ A K
Sbjct: 69  QKPGICLTVSAPGFLNGLTALANATVNGFPMIMISGSSDRAIVDLQQGDYEELDQMNAAK 128

Query: 139 PFSKISVKATDISEIPNCVARVLNSAVSGRPGGCYFDLPSDVLHQTISESEAERLLAAAE 198
           P++K + +     ++   +AR +  +VSGRPGG Y DLP++VL  T+ + EA   +   E
Sbjct: 129 PYAKAAFRVNQPQDLGIALARAIRVSVSGRPGGVYLDLPANVLAATMEKDEALTTIVKVE 188

Query: 199 EFANREVIPRVPNSQIEEAISLLKHAERPLIVFGKGAAIARAEGPLKKLVETTGIPFLPT 258
              +  ++P  P S +  AISLL  AERPLI+ GKGAA ++A+  L++ +E+  IPFLP 
Sbjct: 189 N-PSPALLP-CPKS-VTSAISLLAKAERPLIILGKGAAYSQADEQLREFIESAQIPFLPM 248

Query: 259 PMGKGLLPDTHELAATAARSLAIGKCDVALVVGARLNWLLHFGEPPKWSKDVKFILVDIC 318
            M KG+L DTH L+A AARS A+   DV ++VGARLNWLL  G+   W+ D +FI +DI 
Sbjct: 249 SMAKGILEDTHPLSAAAARSFALANADVVMLVGARLNWLLAHGK-KGWAADTQFIQLDIE 308

Query: 319 KEEVELRKP-HLGLIGDAKEVLESINKEIKDDPFCLGKSHPWVGAISQKAKDNVAKMEVQ 378
            +E++  +P  + ++GD    ++ +  E+K + F       W   ++   + N  KM  +
Sbjct: 309 PQEIDSNRPIAVPVVGDIASSMQGMLAELKQNTFT--TPLVWRDILNIHKQQNAQKMHEK 368

Query: 379 LAKDVVPFNFLTPMRIIRDAILAVGSPAPILVSEGANTMDVGRSVLVQTEPRTRLDAGTW 438
           L+ D  P N+   +  +RD +    +    LV+EGANT+D  R+++   +PR RLD GTW
Sbjct: 369 LSTDTQPLNYFNALSAVRDVLRE--NQDIYLVNEGANTLDNARNIIDMYKPRRRLDCGTW 428

Query: 439 GTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYELPVVVIVFNNSGVYG 498
           G MG+G+GY I A+V S    VVA+EGDS FGFS ME+ET+ RY LPV +++FNN G+Y 
Sbjct: 429 GVMGIGMGYAIGASVTSGSP-VVAIEGDSAFGFSGMEIETICRYNLPVTIVIFNNGGIYR 488

Query: 499 GDRRTPQEITGPYKHDPAPTSFVPSASYDKMIEAFGGKGYYVESPEELKSALAESFSARK 558
           GD     +++G     P+PT  +  A YDK+++AF G GY V + +EL+ AL     +RK
Sbjct: 489 GD---GVDLSG--AGAPSPTDLLHHARYDKLMDAFRGVGYNVTTTDELRHALTTGIQSRK 548

Query: 559 PAVVNVKIDPYAGAESGRLQHKN 579
           P ++NV IDP AG ESG +   N
Sbjct: 549 PTIINVVIDPAAGTESGHITKLN 557

BLAST of IVF0025369 vs. ExPASy TrEMBL
Match: A0A5A7VDZ6 (2-hydroxyacyl-CoA lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold2030G00330 PE=3 SV=1)

HSP 1 Score: 1153.3 bits (2982), Expect = 0.0e+00
Identity = 578/578 (100.00%), Postives = 578/578 (100.00%), Query Frame = 0

Query: 1   MGDSAEALQNDLRFHGPIDGNVLAAMALARCGVDRMFGVVGIPVTSLATRAVSLGIRFIA 60
           MGDSAEALQNDLRFHGPIDGNVLAAMALARCGVDRMFGVVGIPVTSLATRAVSLGIRFIA
Sbjct: 1   MGDSAEALQNDLRFHGPIDGNVLAAMALARCGVDRMFGVVGIPVTSLATRAVSLGIRFIA 60

Query: 61  FHNEQSAGYAASAYGYLTGRPGVLLTVSGPGCVHGLAGISNAMVNAWPLVMISGSCDQRD 120
           FHNEQSAGYAASAYGYLTGRPGVLLTVSGPGCVHGLAGISNAMVNAWPLVMISGSCDQRD
Sbjct: 61  FHNEQSAGYAASAYGYLTGRPGVLLTVSGPGCVHGLAGISNAMVNAWPLVMISGSCDQRD 120

Query: 121 FGRGDFQELDQVEAVKPFSKISVKATDISEIPNCVARVLNSAVSGRPGGCYFDLPSDVLH 180
           FGRGDFQELDQVEAVKPFSKISVKATDISEIPNCVARVLNSAVSGRPGGCYFDLPSDVLH
Sbjct: 121 FGRGDFQELDQVEAVKPFSKISVKATDISEIPNCVARVLNSAVSGRPGGCYFDLPSDVLH 180

Query: 181 QTISESEAERLLAAAEEFANREVIPRVPNSQIEEAISLLKHAERPLIVFGKGAAIARAEG 240
           QTISESEAERLLAAAEEFANREVIPRVPNSQIEEAISLLKHAERPLIVFGKGAAIARAEG
Sbjct: 181 QTISESEAERLLAAAEEFANREVIPRVPNSQIEEAISLLKHAERPLIVFGKGAAIARAEG 240

Query: 241 PLKKLVETTGIPFLPTPMGKGLLPDTHELAATAARSLAIGKCDVALVVGARLNWLLHFGE 300
           PLKKLVETTGIPFLPTPMGKGLLPDTHELAATAARSLAIGKCDVALVVGARLNWLLHFGE
Sbjct: 241 PLKKLVETTGIPFLPTPMGKGLLPDTHELAATAARSLAIGKCDVALVVGARLNWLLHFGE 300

Query: 301 PPKWSKDVKFILVDICKEEVELRKPHLGLIGDAKEVLESINKEIKDDPFCLGKSHPWVGA 360
           PPKWSKDVKFILVDICKEEVELRKPHLGLIGDAKEVLESINKEIKDDPFCLGKSHPWVGA
Sbjct: 301 PPKWSKDVKFILVDICKEEVELRKPHLGLIGDAKEVLESINKEIKDDPFCLGKSHPWVGA 360

Query: 361 ISQKAKDNVAKMEVQLAKDVVPFNFLTPMRIIRDAILAVGSPAPILVSEGANTMDVGRSV 420
           ISQKAKDNVAKMEVQLAKDVVPFNFLTPMRIIRDAILAVGSPAPILVSEGANTMDVGRSV
Sbjct: 361 ISQKAKDNVAKMEVQLAKDVVPFNFLTPMRIIRDAILAVGSPAPILVSEGANTMDVGRSV 420

Query: 421 LVQTEPRTRLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYE 480
           LVQTEPRTRLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYE
Sbjct: 421 LVQTEPRTRLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYE 480

Query: 481 LPVVVIVFNNSGVYGGDRRTPQEITGPYKHDPAPTSFVPSASYDKMIEAFGGKGYYVESP 540
           LPVVVIVFNNSGVYGGDRRTPQEITGPYKHDPAPTSFVPSASYDKMIEAFGGKGYYVESP
Sbjct: 481 LPVVVIVFNNSGVYGGDRRTPQEITGPYKHDPAPTSFVPSASYDKMIEAFGGKGYYVESP 540

Query: 541 EELKSALAESFSARKPAVVNVKIDPYAGAESGRLQHKN 579
           EELKSALAESFSARKPAVVNVKIDPYAGAESGRLQHKN
Sbjct: 541 EELKSALAESFSARKPAVVNVKIDPYAGAESGRLQHKN 578

BLAST of IVF0025369 vs. ExPASy TrEMBL
Match: A0A1S3B9H9 (2-hydroxyacyl-CoA lyase OS=Cucumis melo OX=3656 GN=LOC103487508 PE=3 SV=1)

HSP 1 Score: 1153.3 bits (2982), Expect = 0.0e+00
Identity = 578/578 (100.00%), Postives = 578/578 (100.00%), Query Frame = 0

Query: 1   MGDSAEALQNDLRFHGPIDGNVLAAMALARCGVDRMFGVVGIPVTSLATRAVSLGIRFIA 60
           MGDSAEALQNDLRFHGPIDGNVLAAMALARCGVDRMFGVVGIPVTSLATRAVSLGIRFIA
Sbjct: 1   MGDSAEALQNDLRFHGPIDGNVLAAMALARCGVDRMFGVVGIPVTSLATRAVSLGIRFIA 60

Query: 61  FHNEQSAGYAASAYGYLTGRPGVLLTVSGPGCVHGLAGISNAMVNAWPLVMISGSCDQRD 120
           FHNEQSAGYAASAYGYLTGRPGVLLTVSGPGCVHGLAGISNAMVNAWPLVMISGSCDQRD
Sbjct: 61  FHNEQSAGYAASAYGYLTGRPGVLLTVSGPGCVHGLAGISNAMVNAWPLVMISGSCDQRD 120

Query: 121 FGRGDFQELDQVEAVKPFSKISVKATDISEIPNCVARVLNSAVSGRPGGCYFDLPSDVLH 180
           FGRGDFQELDQVEAVKPFSKISVKATDISEIPNCVARVLNSAVSGRPGGCYFDLPSDVLH
Sbjct: 121 FGRGDFQELDQVEAVKPFSKISVKATDISEIPNCVARVLNSAVSGRPGGCYFDLPSDVLH 180

Query: 181 QTISESEAERLLAAAEEFANREVIPRVPNSQIEEAISLLKHAERPLIVFGKGAAIARAEG 240
           QTISESEAERLLAAAEEFANREVIPRVPNSQIEEAISLLKHAERPLIVFGKGAAIARAEG
Sbjct: 181 QTISESEAERLLAAAEEFANREVIPRVPNSQIEEAISLLKHAERPLIVFGKGAAIARAEG 240

Query: 241 PLKKLVETTGIPFLPTPMGKGLLPDTHELAATAARSLAIGKCDVALVVGARLNWLLHFGE 300
           PLKKLVETTGIPFLPTPMGKGLLPDTHELAATAARSLAIGKCDVALVVGARLNWLLHFGE
Sbjct: 241 PLKKLVETTGIPFLPTPMGKGLLPDTHELAATAARSLAIGKCDVALVVGARLNWLLHFGE 300

Query: 301 PPKWSKDVKFILVDICKEEVELRKPHLGLIGDAKEVLESINKEIKDDPFCLGKSHPWVGA 360
           PPKWSKDVKFILVDICKEEVELRKPHLGLIGDAKEVLESINKEIKDDPFCLGKSHPWVGA
Sbjct: 301 PPKWSKDVKFILVDICKEEVELRKPHLGLIGDAKEVLESINKEIKDDPFCLGKSHPWVGA 360

Query: 361 ISQKAKDNVAKMEVQLAKDVVPFNFLTPMRIIRDAILAVGSPAPILVSEGANTMDVGRSV 420
           ISQKAKDNVAKMEVQLAKDVVPFNFLTPMRIIRDAILAVGSPAPILVSEGANTMDVGRSV
Sbjct: 361 ISQKAKDNVAKMEVQLAKDVVPFNFLTPMRIIRDAILAVGSPAPILVSEGANTMDVGRSV 420

Query: 421 LVQTEPRTRLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYE 480
           LVQTEPRTRLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYE
Sbjct: 421 LVQTEPRTRLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYE 480

Query: 481 LPVVVIVFNNSGVYGGDRRTPQEITGPYKHDPAPTSFVPSASYDKMIEAFGGKGYYVESP 540
           LPVVVIVFNNSGVYGGDRRTPQEITGPYKHDPAPTSFVPSASYDKMIEAFGGKGYYVESP
Sbjct: 481 LPVVVIVFNNSGVYGGDRRTPQEITGPYKHDPAPTSFVPSASYDKMIEAFGGKGYYVESP 540

Query: 541 EELKSALAESFSARKPAVVNVKIDPYAGAESGRLQHKN 579
           EELKSALAESFSARKPAVVNVKIDPYAGAESGRLQHKN
Sbjct: 541 EELKSALAESFSARKPAVVNVKIDPYAGAESGRLQHKN 578

BLAST of IVF0025369 vs. ExPASy TrEMBL
Match: A0A6J1GAR5 (2-hydroxyacyl-CoA lyase-like OS=Cucurbita moschata OX=3662 GN=LOC111452318 PE=3 SV=1)

HSP 1 Score: 1096.6 bits (2835), Expect = 0.0e+00
Identity = 542/578 (93.77%), Postives = 564/578 (97.58%), Query Frame = 0

Query: 1   MGDSAEALQNDLRFHGPIDGNVLAAMALARCGVDRMFGVVGIPVTSLATRAVSLGIRFIA 60
           MGDSA  ++ DL+FHGPIDGNVLAAMALAR GVDRMFGVVGIPVTSLATRAVSLGIRFIA
Sbjct: 1   MGDSAGDVKTDLKFHGPIDGNVLAAMALARFGVDRMFGVVGIPVTSLATRAVSLGIRFIA 60

Query: 61  FHNEQSAGYAASAYGYLTGRPGVLLTVSGPGCVHGLAGISNAMVNAWPLVMISGSCDQRD 120
           FHNEQSAGYAASAYGYLTGRPGVLLTVSGPGCVHGLAG+SNAM NAWPLVMISGSCDQRD
Sbjct: 61  FHNEQSAGYAASAYGYLTGRPGVLLTVSGPGCVHGLAGVSNAMANAWPLVMISGSCDQRD 120

Query: 121 FGRGDFQELDQVEAVKPFSKISVKATDISEIPNCVARVLNSAVSGRPGGCYFDLPSDVLH 180
           FGRGDFQELDQ+EAVKPFSK SVKATDISEIPNCVARVLNSAVSGRPGGCYFDLPSDVLH
Sbjct: 121 FGRGDFQELDQIEAVKPFSKFSVKATDISEIPNCVARVLNSAVSGRPGGCYFDLPSDVLH 180

Query: 181 QTISESEAERLLAAAEEFANREVIPRVPNSQIEEAISLLKHAERPLIVFGKGAAIARAEG 240
           QT++ESEAE LLAAAEEF++REVI RVPNSQIEEAISLLKHAERPLIVFGKGAAIARAEG
Sbjct: 181 QTVTESEAEGLLAAAEEFSHREVIHRVPNSQIEEAISLLKHAERPLIVFGKGAAIARAEG 240

Query: 241 PLKKLVETTGIPFLPTPMGKGLLPDTHELAATAARSLAIGKCDVALVVGARLNWLLHFGE 300
           P+KK VE+TGIPFLPTPMGKGLLPDTH+LAATAARSLAIGKCDVALVVGARLNWLLHFGE
Sbjct: 241 PMKKFVESTGIPFLPTPMGKGLLPDTHKLAATAARSLAIGKCDVALVVGARLNWLLHFGE 300

Query: 301 PPKWSKDVKFILVDICKEEVELRKPHLGLIGDAKEVLESINKEIKDDPFCLGKSHPWVGA 360
           PPKWSKDVKFILVDICKEEVELRKPHLGLIGDAK++LESINKEIKDDPFCLGKSHPWV A
Sbjct: 301 PPKWSKDVKFILVDICKEEVELRKPHLGLIGDAKQILESINKEIKDDPFCLGKSHPWVEA 360

Query: 361 ISQKAKDNVAKMEVQLAKDVVPFNFLTPMRIIRDAILAVGSPAPILVSEGANTMDVGRSV 420
           ISQKA+DNVAKMEVQLA+DVVPFNF+TPMRIIRDAI AVGSPAPILVSEGANTMDVGRSV
Sbjct: 361 ISQKARDNVAKMEVQLARDVVPFNFMTPMRIIRDAISAVGSPAPILVSEGANTMDVGRSV 420

Query: 421 LVQTEPRTRLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYE 480
           LVQTEPRTRLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRY+
Sbjct: 421 LVQTEPRTRLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYQ 480

Query: 481 LPVVVIVFNNSGVYGGDRRTPQEITGPYKHDPAPTSFVPSASYDKMIEAFGGKGYYVESP 540
           LPVVVIVFNN GVYGGDRRTPQEITGPYKHDPAPTSFVPSA+Y KMIEAFGGKGYYVE+P
Sbjct: 481 LPVVVIVFNNGGVYGGDRRTPQEITGPYKHDPAPTSFVPSANYHKMIEAFGGKGYYVETP 540

Query: 541 EELKSALAESFSARKPAVVNVKIDPYAGAESGRLQHKN 579
           EELKSAL+ESFSAR PAV+NVKIDPYAGAESGRLQHKN
Sbjct: 541 EELKSALSESFSARSPAVINVKIDPYAGAESGRLQHKN 578

BLAST of IVF0025369 vs. ExPASy TrEMBL
Match: A0A0A0KW04 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G133700 PE=3 SV=1)

HSP 1 Score: 1096.3 bits (2834), Expect = 0.0e+00
Identity = 552/578 (95.50%), Postives = 559/578 (96.71%), Query Frame = 0

Query: 1   MGDSAEALQNDLRFHGPIDGNVLAAMALARCGVDRMFGVVGIPVTSLATRAVSLGIRFIA 60
           MGDSAEALQNDLRF GPIDGNVLAAMALARCGVDRMFGVVGIPVTSLATRAVSLGIRFIA
Sbjct: 1   MGDSAEALQNDLRFQGPIDGNVLAAMALARCGVDRMFGVVGIPVTSLATRAVSLGIRFIA 60

Query: 61  FHNEQSAGYAASAYGYLTGRPGVLLTVSGPGCVHGLAGISNAMVNAWPLVMISGSCDQRD 120
           FHNEQSAGYAASAYGYLTGRPGVLLTVSGPGCVHGLAG+SNAMVNAWPLVMISGSCDQRD
Sbjct: 61  FHNEQSAGYAASAYGYLTGRPGVLLTVSGPGCVHGLAGVSNAMVNAWPLVMISGSCDQRD 120

Query: 121 FGRGDFQELDQVEAVKPFSKISVKATDISEIPNCVARVLNSAVSGRPGGCYFDLPSDVLH 180
           FGRGDFQELDQVEAVKPFSKISVKATDISEIPNCVARVLNSAVSGRPGGCYFDLPSDVLH
Sbjct: 121 FGRGDFQELDQVEAVKPFSKISVKATDISEIPNCVARVLNSAVSGRPGGCYFDLPSDVLH 180

Query: 181 QTISESEAERLLAAAEEFANREVIPRVPNSQIEEAISLLKHAERPLIVFGKGAAIARAEG 240
           QTISESEAERLL AAEEFA REVIPRVPNSQIEEAISLLKHAERPLIVFGKGAA+ARAEG
Sbjct: 181 QTISESEAERLLVAAEEFARREVIPRVPNSQIEEAISLLKHAERPLIVFGKGAALARAEG 240

Query: 241 PLKKLVETTGIPFLPTPMGKGLLPDTHELAATAARSLAIGKCDVALVVGARLNWLLHFGE 300
           PLKKLVETTGIPFLPTPMG    P        AARSLAIGKCDVALVVGARLNWLLHFGE
Sbjct: 241 PLKKLVETTGIPFLPTPMGGRAPP--------AARSLAIGKCDVALVVGARLNWLLHFGE 300

Query: 301 PPKWSKDVKFILVDICKEEVELRKPHLGLIGDAKEVLESINKEIKDDPFCLGKSHPWVGA 360
           PPKWSKDVKFILVDICKEEVELR PHLGLIGDAKEVLESINKEIKDDPFCLGKSHPWV A
Sbjct: 301 PPKWSKDVKFILVDICKEEVELRNPHLGLIGDAKEVLESINKEIKDDPFCLGKSHPWVEA 360

Query: 361 ISQKAKDNVAKMEVQLAKDVVPFNFLTPMRIIRDAILAVGSPAPILVSEGANTMDVGRSV 420
           ISQKAKDNVAKMEVQLA+DVVPFNFLTPMRIIRDAILA+GSPAPILVSEGANTMDVGRSV
Sbjct: 361 ISQKAKDNVAKMEVQLARDVVPFNFLTPMRIIRDAILALGSPAPILVSEGANTMDVGRSV 420

Query: 421 LVQTEPRTRLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYE 480
           LVQTEPRTRLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRY+
Sbjct: 421 LVQTEPRTRLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYQ 480

Query: 481 LPVVVIVFNNSGVYGGDRRTPQEITGPYKHDPAPTSFVPSASYDKMIEAFGGKGYYVESP 540
           LPVVVIVFNNSGVYGGDRRTPQEITGPYKHDPAPTSFVPSA Y KMIEAFGGKGY+VESP
Sbjct: 481 LPVVVIVFNNSGVYGGDRRTPQEITGPYKHDPAPTSFVPSAGYHKMIEAFGGKGYHVESP 540

Query: 541 EELKSALAESFSARKPAVVNVKIDPYAGAESGRLQHKN 579
           EELKSALAESFSARKPAVVN+KIDPYAGAESGRLQHKN
Sbjct: 541 EELKSALAESFSARKPAVVNIKIDPYAGAESGRLQHKN 570

BLAST of IVF0025369 vs. ExPASy TrEMBL
Match: A0A6J1KDD5 (2-hydroxyacyl-CoA lyase OS=Cucurbita maxima OX=3661 GN=LOC111492838 PE=3 SV=1)

HSP 1 Score: 1095.9 bits (2833), Expect = 0.0e+00
Identity = 540/578 (93.43%), Postives = 565/578 (97.75%), Query Frame = 0

Query: 1   MGDSAEALQNDLRFHGPIDGNVLAAMALARCGVDRMFGVVGIPVTSLATRAVSLGIRFIA 60
           MGDSA  ++ DL+FHGPIDGNVLAAMALAR GVDRMFGVVGIPVTSLATRAVSLGIRFIA
Sbjct: 1   MGDSAGDVKTDLKFHGPIDGNVLAAMALARFGVDRMFGVVGIPVTSLATRAVSLGIRFIA 60

Query: 61  FHNEQSAGYAASAYGYLTGRPGVLLTVSGPGCVHGLAGISNAMVNAWPLVMISGSCDQRD 120
           FHNEQSAGYAASAYGYLTGRPGVLLTVSGPGCVHGLAG+SNAM NAWPLVMISGSCDQRD
Sbjct: 61  FHNEQSAGYAASAYGYLTGRPGVLLTVSGPGCVHGLAGVSNAMANAWPLVMISGSCDQRD 120

Query: 121 FGRGDFQELDQVEAVKPFSKISVKATDISEIPNCVARVLNSAVSGRPGGCYFDLPSDVLH 180
           FGRGDFQELDQ+EAVKPFSK SVKATDISEIPNCVARVLNSAVSGRPGGCYFDLPSDVLH
Sbjct: 121 FGRGDFQELDQIEAVKPFSKFSVKATDISEIPNCVARVLNSAVSGRPGGCYFDLPSDVLH 180

Query: 181 QTISESEAERLLAAAEEFANREVIPRVPNSQIEEAISLLKHAERPLIVFGKGAAIARAEG 240
           QT++E+EAERLLAAAEEF++REVI RVPNSQIEEAISLLK AERPLIVFGKGAAIARAEG
Sbjct: 181 QTVTETEAERLLAAAEEFSHREVIHRVPNSQIEEAISLLKRAERPLIVFGKGAAIARAEG 240

Query: 241 PLKKLVETTGIPFLPTPMGKGLLPDTHELAATAARSLAIGKCDVALVVGARLNWLLHFGE 300
           PLKKLVE+TGIPFLPTPMGKGLLPDTH+LAATAARSLAIGKCDVALVVGARLNWLLHFGE
Sbjct: 241 PLKKLVESTGIPFLPTPMGKGLLPDTHQLAATAARSLAIGKCDVALVVGARLNWLLHFGE 300

Query: 301 PPKWSKDVKFILVDICKEEVELRKPHLGLIGDAKEVLESINKEIKDDPFCLGKSHPWVGA 360
           PPKWSKDVKFILVDICKEEVE+RKPHLGLIGDAK++LESINKEIKDDPFCLGKSHPWV A
Sbjct: 301 PPKWSKDVKFILVDICKEEVEMRKPHLGLIGDAKQILESINKEIKDDPFCLGKSHPWVEA 360

Query: 361 ISQKAKDNVAKMEVQLAKDVVPFNFLTPMRIIRDAILAVGSPAPILVSEGANTMDVGRSV 420
           ISQKA+DNVAKMEVQLA+DVVPFNF+TPMRIIRDAI A+GSPAPILVSEGANTMDVGRSV
Sbjct: 361 ISQKARDNVAKMEVQLARDVVPFNFMTPMRIIRDAITALGSPAPILVSEGANTMDVGRSV 420

Query: 421 LVQTEPRTRLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYE 480
           LVQTEPRTRLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRY+
Sbjct: 421 LVQTEPRTRLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYQ 480

Query: 481 LPVVVIVFNNSGVYGGDRRTPQEITGPYKHDPAPTSFVPSASYDKMIEAFGGKGYYVESP 540
           LPV+VIVFNN GVYGGDRRTPQEITGPYKHDPAPTSFVPSA+Y KMIEAFGGKGYYVE+P
Sbjct: 481 LPVIVIVFNNGGVYGGDRRTPQEITGPYKHDPAPTSFVPSANYHKMIEAFGGKGYYVETP 540

Query: 541 EELKSALAESFSARKPAVVNVKIDPYAGAESGRLQHKN 579
           EELKSAL+ESFSAR PAV+NVKIDPYAGAESGRLQHKN
Sbjct: 541 EELKSALSESFSARSPAVINVKIDPYAGAESGRLQHKN 578

BLAST of IVF0025369 vs. NCBI nr
Match: XP_008444073.1 (PREDICTED: 2-hydroxyacyl-CoA lyase [Cucumis melo] >KAA0064125.1 2-hydroxyacyl-CoA lyase [Cucumis melo var. makuwa] >TYK28615.1 2-hydroxyacyl-CoA lyase [Cucumis melo var. makuwa])

HSP 1 Score: 1151 bits (2977), Expect = 0.0
Identity = 578/578 (100.00%), Postives = 578/578 (100.00%), Query Frame = 0

Query: 1   MGDSAEALQNDLRFHGPIDGNVLAAMALARCGVDRMFGVVGIPVTSLATRAVSLGIRFIA 60
           MGDSAEALQNDLRFHGPIDGNVLAAMALARCGVDRMFGVVGIPVTSLATRAVSLGIRFIA
Sbjct: 1   MGDSAEALQNDLRFHGPIDGNVLAAMALARCGVDRMFGVVGIPVTSLATRAVSLGIRFIA 60

Query: 61  FHNEQSAGYAASAYGYLTGRPGVLLTVSGPGCVHGLAGISNAMVNAWPLVMISGSCDQRD 120
           FHNEQSAGYAASAYGYLTGRPGVLLTVSGPGCVHGLAGISNAMVNAWPLVMISGSCDQRD
Sbjct: 61  FHNEQSAGYAASAYGYLTGRPGVLLTVSGPGCVHGLAGISNAMVNAWPLVMISGSCDQRD 120

Query: 121 FGRGDFQELDQVEAVKPFSKISVKATDISEIPNCVARVLNSAVSGRPGGCYFDLPSDVLH 180
           FGRGDFQELDQVEAVKPFSKISVKATDISEIPNCVARVLNSAVSGRPGGCYFDLPSDVLH
Sbjct: 121 FGRGDFQELDQVEAVKPFSKISVKATDISEIPNCVARVLNSAVSGRPGGCYFDLPSDVLH 180

Query: 181 QTISESEAERLLAAAEEFANREVIPRVPNSQIEEAISLLKHAERPLIVFGKGAAIARAEG 240
           QTISESEAERLLAAAEEFANREVIPRVPNSQIEEAISLLKHAERPLIVFGKGAAIARAEG
Sbjct: 181 QTISESEAERLLAAAEEFANREVIPRVPNSQIEEAISLLKHAERPLIVFGKGAAIARAEG 240

Query: 241 PLKKLVETTGIPFLPTPMGKGLLPDTHELAATAARSLAIGKCDVALVVGARLNWLLHFGE 300
           PLKKLVETTGIPFLPTPMGKGLLPDTHELAATAARSLAIGKCDVALVVGARLNWLLHFGE
Sbjct: 241 PLKKLVETTGIPFLPTPMGKGLLPDTHELAATAARSLAIGKCDVALVVGARLNWLLHFGE 300

Query: 301 PPKWSKDVKFILVDICKEEVELRKPHLGLIGDAKEVLESINKEIKDDPFCLGKSHPWVGA 360
           PPKWSKDVKFILVDICKEEVELRKPHLGLIGDAKEVLESINKEIKDDPFCLGKSHPWVGA
Sbjct: 301 PPKWSKDVKFILVDICKEEVELRKPHLGLIGDAKEVLESINKEIKDDPFCLGKSHPWVGA 360

Query: 361 ISQKAKDNVAKMEVQLAKDVVPFNFLTPMRIIRDAILAVGSPAPILVSEGANTMDVGRSV 420
           ISQKAKDNVAKMEVQLAKDVVPFNFLTPMRIIRDAILAVGSPAPILVSEGANTMDVGRSV
Sbjct: 361 ISQKAKDNVAKMEVQLAKDVVPFNFLTPMRIIRDAILAVGSPAPILVSEGANTMDVGRSV 420

Query: 421 LVQTEPRTRLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYE 480
           LVQTEPRTRLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYE
Sbjct: 421 LVQTEPRTRLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYE 480

Query: 481 LPVVVIVFNNSGVYGGDRRTPQEITGPYKHDPAPTSFVPSASYDKMIEAFGGKGYYVESP 540
           LPVVVIVFNNSGVYGGDRRTPQEITGPYKHDPAPTSFVPSASYDKMIEAFGGKGYYVESP
Sbjct: 481 LPVVVIVFNNSGVYGGDRRTPQEITGPYKHDPAPTSFVPSASYDKMIEAFGGKGYYVESP 540

Query: 541 EELKSALAESFSARKPAVVNVKIDPYAGAESGRLQHKN 578
           EELKSALAESFSARKPAVVNVKIDPYAGAESGRLQHKN
Sbjct: 541 EELKSALAESFSARKPAVVNVKIDPYAGAESGRLQHKN 578

BLAST of IVF0025369 vs. NCBI nr
Match: XP_011653421.1 (2-hydroxyacyl-CoA lyase [Cucumis sativus] >KAE8649340.1 hypothetical protein Csa_014526 [Cucumis sativus])

HSP 1 Score: 1128 bits (2917), Expect = 0.0
Identity = 564/578 (97.58%), Postives = 571/578 (98.79%), Query Frame = 0

Query: 1   MGDSAEALQNDLRFHGPIDGNVLAAMALARCGVDRMFGVVGIPVTSLATRAVSLGIRFIA 60
           MGDSAEALQNDLRF GPIDGNVLAAMALARCGVDRMFGVVGIPVTSLATRAVSLGIRFIA
Sbjct: 1   MGDSAEALQNDLRFQGPIDGNVLAAMALARCGVDRMFGVVGIPVTSLATRAVSLGIRFIA 60

Query: 61  FHNEQSAGYAASAYGYLTGRPGVLLTVSGPGCVHGLAGISNAMVNAWPLVMISGSCDQRD 120
           FHNEQSAGYAASAYGYLTGRPGVLLTVSGPGCVHGLAG+SNAMVNAWPLVMISGSCDQRD
Sbjct: 61  FHNEQSAGYAASAYGYLTGRPGVLLTVSGPGCVHGLAGVSNAMVNAWPLVMISGSCDQRD 120

Query: 121 FGRGDFQELDQVEAVKPFSKISVKATDISEIPNCVARVLNSAVSGRPGGCYFDLPSDVLH 180
           FGRGDFQELDQVEAVKPFSKISVKATDISEIPNCVARVLNSAVSGRPGGCYFDLPSDVLH
Sbjct: 121 FGRGDFQELDQVEAVKPFSKISVKATDISEIPNCVARVLNSAVSGRPGGCYFDLPSDVLH 180

Query: 181 QTISESEAERLLAAAEEFANREVIPRVPNSQIEEAISLLKHAERPLIVFGKGAAIARAEG 240
           QTISESEAERLL AAEEFA REVIPRVPNSQIEEAISLLKHAERPLIVFGKGAA+ARAEG
Sbjct: 181 QTISESEAERLLVAAEEFARREVIPRVPNSQIEEAISLLKHAERPLIVFGKGAALARAEG 240

Query: 241 PLKKLVETTGIPFLPTPMGKGLLPDTHELAATAARSLAIGKCDVALVVGARLNWLLHFGE 300
           PLKKLVETTGIPFLPTPMGKGLLPDTHELAATAARSLAIGKCDVALVVGARLNWLLHFGE
Sbjct: 241 PLKKLVETTGIPFLPTPMGKGLLPDTHELAATAARSLAIGKCDVALVVGARLNWLLHFGE 300

Query: 301 PPKWSKDVKFILVDICKEEVELRKPHLGLIGDAKEVLESINKEIKDDPFCLGKSHPWVGA 360
           PPKWSKDVKFILVDICKEEVELR PHLGLIGDAKEVLESINKEIKDDPFCLGKSHPWV A
Sbjct: 301 PPKWSKDVKFILVDICKEEVELRNPHLGLIGDAKEVLESINKEIKDDPFCLGKSHPWVEA 360

Query: 361 ISQKAKDNVAKMEVQLAKDVVPFNFLTPMRIIRDAILAVGSPAPILVSEGANTMDVGRSV 420
           ISQKAKDNVAKMEVQLA+DVVPFNFLTPMRIIRDAILA+GSPAPILVSEGANTMDVGRSV
Sbjct: 361 ISQKAKDNVAKMEVQLARDVVPFNFLTPMRIIRDAILALGSPAPILVSEGANTMDVGRSV 420

Query: 421 LVQTEPRTRLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYE 480
           LVQTEPRTRLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRY+
Sbjct: 421 LVQTEPRTRLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYQ 480

Query: 481 LPVVVIVFNNSGVYGGDRRTPQEITGPYKHDPAPTSFVPSASYDKMIEAFGGKGYYVESP 540
           LPVVVIVFNNSGVYGGDRRTPQEITGPYKHDPAPTSFVPSA Y KMIEAFGGKGY+VESP
Sbjct: 481 LPVVVIVFNNSGVYGGDRRTPQEITGPYKHDPAPTSFVPSAGYHKMIEAFGGKGYHVESP 540

Query: 541 EELKSALAESFSARKPAVVNVKIDPYAGAESGRLQHKN 578
           EELKSALAESFSARKPAVVN+KIDPYAGAESGRLQHKN
Sbjct: 541 EELKSALAESFSARKPAVVNIKIDPYAGAESGRLQHKN 578

BLAST of IVF0025369 vs. NCBI nr
Match: XP_038893948.1 (2-hydroxyacyl-CoA lyase [Benincasa hispida])

HSP 1 Score: 1114 bits (2882), Expect = 0.0
Identity = 557/578 (96.37%), Postives = 568/578 (98.27%), Query Frame = 0

Query: 1   MGDSAEALQNDLRFHGPIDGNVLAAMALARCGVDRMFGVVGIPVTSLATRAVSLGIRFIA 60
           MGDS+E LQNDL+FHGPIDGNV AAMALAR GVDRMFGVVGIPVTSLATRAVSLGIRFIA
Sbjct: 1   MGDSSEDLQNDLKFHGPIDGNVFAAMALARYGVDRMFGVVGIPVTSLATRAVSLGIRFIA 60

Query: 61  FHNEQSAGYAASAYGYLTGRPGVLLTVSGPGCVHGLAGISNAMVNAWPLVMISGSCDQRD 120
           FHNEQSAGYAASAYGYLTG PGVLLTVSGPGCVHGLAG+SNAMVNAWPLVMISGSCDQRD
Sbjct: 61  FHNEQSAGYAASAYGYLTGSPGVLLTVSGPGCVHGLAGVSNAMVNAWPLVMISGSCDQRD 120

Query: 121 FGRGDFQELDQVEAVKPFSKISVKATDISEIPNCVARVLNSAVSGRPGGCYFDLPSDVLH 180
           FGRGDFQELDQVEAVKPFSKISVKATDISEIPNCVARVLNSAVSGRPGGCYFDLPSDVLH
Sbjct: 121 FGRGDFQELDQVEAVKPFSKISVKATDISEIPNCVARVLNSAVSGRPGGCYFDLPSDVLH 180

Query: 181 QTISESEAERLLAAAEEFANREVIPRVPNSQIEEAISLLKHAERPLIVFGKGAAIARAEG 240
           QT+SESEAERLLAAAEEF +REVI RVPNSQIEEAISLLKHAERPLIVFGKGAAIARAEG
Sbjct: 181 QTVSESEAERLLAAAEEFTHREVIHRVPNSQIEEAISLLKHAERPLIVFGKGAAIARAEG 240

Query: 241 PLKKLVETTGIPFLPTPMGKGLLPDTHELAATAARSLAIGKCDVALVVGARLNWLLHFGE 300
           PLKKLVE+TGIPFLPTPMGKGLLPDTHELAATAARSLAIGKCDVALVVGARLNWLLHFGE
Sbjct: 241 PLKKLVESTGIPFLPTPMGKGLLPDTHELAATAARSLAIGKCDVALVVGARLNWLLHFGE 300

Query: 301 PPKWSKDVKFILVDICKEEVELRKPHLGLIGDAKEVLESINKEIKDDPFCLGKSHPWVGA 360
           PPKWSKDVKFILVDICKEEVELRKPHLGLIGDAKEVLESINKEIKDDPFCLGKSHPWV A
Sbjct: 301 PPKWSKDVKFILVDICKEEVELRKPHLGLIGDAKEVLESINKEIKDDPFCLGKSHPWVEA 360

Query: 361 ISQKAKDNVAKMEVQLAKDVVPFNFLTPMRIIRDAILAVGSPAPILVSEGANTMDVGRSV 420
           ISQKAKDNVAKMEVQLA+DVVPFNFLTPMRIIRDAI A+GSPAPILVSEGANTMDVGRSV
Sbjct: 361 ISQKAKDNVAKMEVQLARDVVPFNFLTPMRIIRDAISALGSPAPILVSEGANTMDVGRSV 420

Query: 421 LVQTEPRTRLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYE 480
           LVQTEPRTRLDAGTWGTMG+GLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRY+
Sbjct: 421 LVQTEPRTRLDAGTWGTMGIGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYQ 480

Query: 481 LPVVVIVFNNSGVYGGDRRTPQEITGPYKHDPAPTSFVPSASYDKMIEAFGGKGYYVESP 540
           LPVVVIVFNN GVYGGDRRTPQEITGPYKHDPAPTSFVPSASY KMIEAFGGKGYYVE+P
Sbjct: 481 LPVVVIVFNNGGVYGGDRRTPQEITGPYKHDPAPTSFVPSASYHKMIEAFGGKGYYVETP 540

Query: 541 EELKSALAESFSARKPAVVNVKIDPYAGAESGRLQHKN 578
           EELKSALAESFSARKPAVVNVKIDPYAGAESGRLQHKN
Sbjct: 541 EELKSALAESFSARKPAVVNVKIDPYAGAESGRLQHKN 578

BLAST of IVF0025369 vs. NCBI nr
Match: XP_023524913.1 (2-hydroxyacyl-CoA lyase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1100 bits (2844), Expect = 0.0
Identity = 545/578 (94.29%), Postives = 566/578 (97.92%), Query Frame = 0

Query: 1   MGDSAEALQNDLRFHGPIDGNVLAAMALARCGVDRMFGVVGIPVTSLATRAVSLGIRFIA 60
           MGDSA  ++ DL+FHGPIDGNVLAAMALAR GVDRMFGVVGIPVTSLATRAVSLGIRFIA
Sbjct: 1   MGDSAGDVKTDLKFHGPIDGNVLAAMALARFGVDRMFGVVGIPVTSLATRAVSLGIRFIA 60

Query: 61  FHNEQSAGYAASAYGYLTGRPGVLLTVSGPGCVHGLAGISNAMVNAWPLVMISGSCDQRD 120
           FHNEQSAGYAASAYGYLTGRPGVLLTVSGPGCVHGLAG+SNAM NAWPLVMISGSCDQRD
Sbjct: 61  FHNEQSAGYAASAYGYLTGRPGVLLTVSGPGCVHGLAGVSNAMANAWPLVMISGSCDQRD 120

Query: 121 FGRGDFQELDQVEAVKPFSKISVKATDISEIPNCVARVLNSAVSGRPGGCYFDLPSDVLH 180
           FGRGDFQELDQ+EAVKPFSK SVKATDISEIPNCVARVLNSAVSGRPGGCYFDLPSDVLH
Sbjct: 121 FGRGDFQELDQIEAVKPFSKFSVKATDISEIPNCVARVLNSAVSGRPGGCYFDLPSDVLH 180

Query: 181 QTISESEAERLLAAAEEFANREVIPRVPNSQIEEAISLLKHAERPLIVFGKGAAIARAEG 240
           QT+SESEAERLLAAAEEF++REVI RVPNSQIEEAISLLKHAERPLIVFGKGAAIARAEG
Sbjct: 181 QTVSESEAERLLAAAEEFSHREVIHRVPNSQIEEAISLLKHAERPLIVFGKGAAIARAEG 240

Query: 241 PLKKLVETTGIPFLPTPMGKGLLPDTHELAATAARSLAIGKCDVALVVGARLNWLLHFGE 300
           PLKKLVE+TGIPFLPTPMGKGLLPDTH+LAATAARSLAIGKCDVALVVGARLNWLLHFGE
Sbjct: 241 PLKKLVESTGIPFLPTPMGKGLLPDTHQLAATAARSLAIGKCDVALVVGARLNWLLHFGE 300

Query: 301 PPKWSKDVKFILVDICKEEVELRKPHLGLIGDAKEVLESINKEIKDDPFCLGKSHPWVGA 360
           PPKWSKDVKFILVDICKEEVELRKPHLGLIGDAK++LESINKEIKDDPFCLGKSHPWV A
Sbjct: 301 PPKWSKDVKFILVDICKEEVELRKPHLGLIGDAKQILESINKEIKDDPFCLGKSHPWVEA 360

Query: 361 ISQKAKDNVAKMEVQLAKDVVPFNFLTPMRIIRDAILAVGSPAPILVSEGANTMDVGRSV 420
           ISQKA+DNVAKMEVQLA+DVVPFNF+TPMRIIRDAI AVGSP+PILVSEGANTMDVGRSV
Sbjct: 361 ISQKARDNVAKMEVQLARDVVPFNFMTPMRIIRDAISAVGSPSPILVSEGANTMDVGRSV 420

Query: 421 LVQTEPRTRLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYE 480
           LVQTEPRTRLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRY+
Sbjct: 421 LVQTEPRTRLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYQ 480

Query: 481 LPVVVIVFNNSGVYGGDRRTPQEITGPYKHDPAPTSFVPSASYDKMIEAFGGKGYYVESP 540
           LPVVVIVFNN GVYGGDRRTPQEITGPYKHDPAPTSFVPSA+Y KMIEAFGGKGYYVE+P
Sbjct: 481 LPVVVIVFNNGGVYGGDRRTPQEITGPYKHDPAPTSFVPSANYHKMIEAFGGKGYYVETP 540

Query: 541 EELKSALAESFSARKPAVVNVKIDPYAGAESGRLQHKN 578
           EELKSAL+ESFSAR PAV+NVKIDPYAGAESGRLQHKN
Sbjct: 541 EELKSALSESFSARNPAVINVKIDPYAGAESGRLQHKN 578

BLAST of IVF0025369 vs. NCBI nr
Match: KAG7036762.1 (2-hydroxyacyl-CoA lyase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1095 bits (2833), Expect = 0.0
Identity = 543/578 (93.94%), Postives = 565/578 (97.75%), Query Frame = 0

Query: 1   MGDSAEALQNDLRFHGPIDGNVLAAMALARCGVDRMFGVVGIPVTSLATRAVSLGIRFIA 60
           MGDSA  ++ DL+FHGPIDGNVLAAMALAR GVDRMFGVVGIPVTSLATRAVSLGIRFIA
Sbjct: 1   MGDSAGDVRADLKFHGPIDGNVLAAMALARFGVDRMFGVVGIPVTSLATRAVSLGIRFIA 60

Query: 61  FHNEQSAGYAASAYGYLTGRPGVLLTVSGPGCVHGLAGISNAMVNAWPLVMISGSCDQRD 120
           FHNEQSAGYAASAYGYLTGRPGVLLTVSGPGCVHGLAG+SNAM NAWPLVMISGSCDQRD
Sbjct: 61  FHNEQSAGYAASAYGYLTGRPGVLLTVSGPGCVHGLAGVSNAMANAWPLVMISGSCDQRD 120

Query: 121 FGRGDFQELDQVEAVKPFSKISVKATDISEIPNCVARVLNSAVSGRPGGCYFDLPSDVLH 180
           FGRGDFQELDQ+EAVKPFSK SVKATDISEIPNCVARVLNSAVSGRPGGCYFDLPSDVLH
Sbjct: 121 FGRGDFQELDQIEAVKPFSKFSVKATDISEIPNCVARVLNSAVSGRPGGCYFDLPSDVLH 180

Query: 181 QTISESEAERLLAAAEEFANREVIPRVPNSQIEEAISLLKHAERPLIVFGKGAAIARAEG 240
           QT++ESEAERLLAAAEEF++REVI RVPNSQIEEAISLLK AERPLIVFGKGAAIARAEG
Sbjct: 181 QTVTESEAERLLAAAEEFSHREVIHRVPNSQIEEAISLLKRAERPLIVFGKGAAIARAEG 240

Query: 241 PLKKLVETTGIPFLPTPMGKGLLPDTHELAATAARSLAIGKCDVALVVGARLNWLLHFGE 300
           PLKKLVE+TGIPFLPTPMGKGLLPDTH+LAATAARSLAIGKCDVALVVGARLNWLLHFGE
Sbjct: 241 PLKKLVESTGIPFLPTPMGKGLLPDTHQLAATAARSLAIGKCDVALVVGARLNWLLHFGE 300

Query: 301 PPKWSKDVKFILVDICKEEVELRKPHLGLIGDAKEVLESINKEIKDDPFCLGKSHPWVGA 360
           PPKWSKDVKFIL+DICKEEVELRKPHLGLIGDAK++LESINKEIKDDPFCLGKSHPWV A
Sbjct: 301 PPKWSKDVKFILIDICKEEVELRKPHLGLIGDAKQILESINKEIKDDPFCLGKSHPWVEA 360

Query: 361 ISQKAKDNVAKMEVQLAKDVVPFNFLTPMRIIRDAILAVGSPAPILVSEGANTMDVGRSV 420
           ISQKA+DNVAKMEVQLA+DVVPFNF+TPMRIIRDAI AVGSPAPILVSEGANTMDVGRSV
Sbjct: 361 ISQKARDNVAKMEVQLARDVVPFNFMTPMRIIRDAISAVGSPAPILVSEGANTMDVGRSV 420

Query: 421 LVQTEPRTRLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYE 480
           LVQTEPRTRLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRY+
Sbjct: 421 LVQTEPRTRLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYQ 480

Query: 481 LPVVVIVFNNSGVYGGDRRTPQEITGPYKHDPAPTSFVPSASYDKMIEAFGGKGYYVESP 540
           LPVVVIVFNN GVYGGDRRTPQEITGPYKHDPAPTSFVPSA+Y KMIEAFGGKGYYVE+P
Sbjct: 481 LPVVVIVFNNGGVYGGDRRTPQEITGPYKHDPAPTSFVPSANYHKMIEAFGGKGYYVETP 540

Query: 541 EELKSALAESFSARKPAVVNVKIDPYAGAESGRLQHKN 578
           EELKSAL+ESFSAR PAV+NVKIDPYAGAESGRLQHKN
Sbjct: 541 EELKSALSESFSARSPAVINVKIDPYAGAESGRLQHKN 578

BLAST of IVF0025369 vs. TAIR 10
Match: AT5G17380.1 (Thiamine pyrophosphate dependent pyruvate decarboxylase family protein )

HSP 1 Score: 915.6 bits (2365), Expect = 2.0e-266
Identity = 450/561 (80.21%), Postives = 501/561 (89.30%), Query Frame = 0

Query: 18  IDGNVLAAMALARCGVDRMFGVVGIPVTSLATRAVSLGIRFIAFHNEQSAGYAASAYGYL 77
           IDGNVL A +L+  GV  MFGVVGIPVTSLA+RA++LGIRFIAFHNEQSAGYAASAYGYL
Sbjct: 12  IDGNVLVAKSLSHLGVTHMFGVVGIPVTSLASRAMALGIRFIAFHNEQSAGYAASAYGYL 71

Query: 78  TGRPGVLLTVSGPGCVHGLAGISNAMVNAWPLVMISGSCDQRDFGRGDFQELDQVEAVKP 137
           TG+PG+LLTVSGPGCVHGLAG+SNA VN WP+VMISGSCDQRD GRGDFQELDQ+EAVK 
Sbjct: 72  TGKPGILLTVSGPGCVHGLAGLSNAWVNTWPMVMISGSCDQRDVGRGDFQELDQIEAVKA 131

Query: 138 FSKISVKATDISEIPNCVARVLNSAVSGRPGGCYFDLPSDVLHQTISESEAERLLAAAEE 197
           FSK+S KA D+ EIP+CV+RVL+ AVSGRPGGCY D+P+DVL Q ISESEA++L+   E 
Sbjct: 132 FSKLSEKAKDVREIPDCVSRVLDRAVSGRPGGCYLDIPTDVLRQKISESEADKLVDEVER 191

Query: 198 FANREVIPRVPNSQIEEAISLLKHAERPLIVFGKGAAIARAEGPLKKLVETTGIPFLPTP 257
               E I     S+IE A+SLL+ AERPLIVFGKGAA +RAE  LKKLVE TGIPFLPTP
Sbjct: 192 SRKEEPIRGSLRSEIESAVSLLRKAERPLIVFGKGAAYSRAEDELKKLVEITGIPFLPTP 251

Query: 258 MGKGLLPDTHELAATAARSLAIGKCDVALVVGARLNWLLHFGEPPKWSKDVKFILVDICK 317
           MGKGLLPDTHE +ATAARSLAIGKCDVALVVGARLNWLLHFGE PKW KDVKFILVD+ +
Sbjct: 252 MGKGLLPDTHEFSATAARSLAIGKCDVALVVGARLNWLLHFGESPKWDKDVKFILVDVSE 311

Query: 318 EEVELRKPHLGLIGDAKEVLESINKEIKDDPFCLGKSHPWVGAISQKAKDNVAKMEVQLA 377
           EE+ELRKPHLG++GDAK V+  +N+EIKDDPFCLGKS+ WV +IS+KAK+N  KME+QLA
Sbjct: 312 EEIELRKPHLGIVGDAKTVIGLLNREIKDDPFCLGKSNSWVESISKKAKENGEKMEIQLA 371

Query: 378 KDVVPFNFLTPMRIIRDAILAVGSPAPILVSEGANTMDVGRSVLVQTEPRTRLDAGTWGT 437
           KDVVPFNFLTPMRIIRDAILAV  P+P++VSEGANTMDVGRSVLVQ EPRTRLDAGTWGT
Sbjct: 372 KDVVPFNFLTPMRIIRDAILAVEGPSPVVVSEGANTMDVGRSVLVQKEPRTRLDAGTWGT 431

Query: 438 MGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYELPVVVIVFNNSGVYGGD 497
           MGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRY L VV+IVFNN GVYGGD
Sbjct: 432 MGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYNLAVVIIVFNNGGVYGGD 491

Query: 498 RRTPQEITGPYKHDPAPTSFVPSASYDKMIEAFGGKGYYVESPEELKSALAESFSARKPA 557
           RR P+EI+GP+K DPAPTSFVP+A Y K+IEAFGGKGY VE+P+ELKSALAESF+ARKPA
Sbjct: 492 RRGPEEISGPHKEDPAPTSFVPNAGYHKLIEAFGGKGYIVETPDELKSALAESFAARKPA 551

Query: 558 VVNVKIDPYAGAESGRLQHKN 579
           VVNV IDP+AGAESGRLQHKN
Sbjct: 552 VVNVIIDPFAGAESGRLQHKN 572

BLAST of IVF0025369 vs. TAIR 10
Match: AT3G48560.1 (chlorsulfuron/imidazolinone resistant 1 )

HSP 1 Score: 178.7 bits (452), Expect = 1.3e-44
Identity = 142/495 (28.69%), Postives = 235/495 (47.47%), Query Frame = 0

Query: 17  PIDGNVLAAMALARCGVDRMF---GVVGIPVTSLATRAVSLGIRFIAFHNEQSAGYAASA 76
           P  G  +   AL R GV+ +F   G   + +    TR+ S  IR +   +EQ   +AA  
Sbjct: 96  PRKGADILVEALERQGVETVFAYPGGASMEIHQALTRSSS--IRNVLPRHEQGGVFAAEG 155

Query: 77  YGYLTGRPGVLLTVSGPGCVHGLAGISNAMVNAWPLVMISGSCDQRDFGRGDFQELDQVE 136
           Y   +G+PG+ +  SGPG  + ++G+++A++++ PLV I+G   +R  G   FQE   VE
Sbjct: 156 YARSSGKPGICIATSGPGATNLVSGLADALLDSVPLVAITGQVPRRMIGTDAFQETPIVE 215

Query: 137 AVKPFSKISVKATDISEIPNCVARVLNSAVSGRPGGCYFDLPSDVLHQTI--SESEAERL 196
             +  +K +    D+ +IP  +      A SGRPG    D+P D+  Q    +  +A RL
Sbjct: 216 VTRSITKHNYLVMDVEDIPRIIEEAFFLATSGRPGPVLVDVPKDIQQQLAIPNWEQAMRL 275

Query: 197 LAAAEEFANREVIPRVP-NSQIEEAISLLKHAERPLIVFGKGAAIARAEGPLKKLVETTG 256
                 + +R  +P+ P +S +E+ + L+  +++P++  G G   +  E  L + VE TG
Sbjct: 276 ----PGYMSR--MPKPPEDSHLEQIVRLISESKKPVLYVGGGCLNSSDE--LGRFVELTG 335

Query: 257 IPFLPTPMGKGLLPDTHELA-------ATAARSLAIGKCDVALVVGARLNWLLHFGEPPK 316
           IP   T MG G  P   EL+        T   + A+   D+ L  G R +  +  G+   
Sbjct: 336 IPVASTLMGLGSYPCDDELSLHMLGMHGTVYANYAVEHSDLLLAFGVRFDDRV-TGKLEA 395

Query: 317 WSKDVKFILVDICKEEVELRK-PHLGLIGDAKEVLESINKEIKDDPFCLGKSHPWVGAIS 376
           ++   K + +DI   E+   K PH+ + GD K  L+ +NK +++             A  
Sbjct: 396 FASRAKIVHIDIDSAEIGKNKTPHVSVCGDVKLALQGMNKVLEN------------RAEE 455

Query: 377 QKAKDNVAKMEVQLAKDVVPFNFLT------PMRIIRDAILAVGSPAPILVSEGANTMDV 436
            K    V + E+ + K   P +F T      P   I+  +L   +    ++S G     +
Sbjct: 456 LKLDFGVWRNELNVQKQKFPLSFKTFGEAIPPQYAIK--VLDELTDGKAIISTGVGQHQM 515

Query: 437 GRSVLVQ-TEPRTRLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVET 491
             +      +PR  L +G  G MG GL   I A+VA+PD +VV ++GD  F  +  E+ T
Sbjct: 516 WAAQFYNYKKPRQWLSSGGLGAMGFGLPAAIGASVANPDAIVVDIDGDGSFIMNVQELAT 565

BLAST of IVF0025369 vs. TAIR 10
Match: AT4G33070.1 (Thiamine pyrophosphate dependent pyruvate decarboxylase family protein )

HSP 1 Score: 63.9 bits (154), Expect = 4.8e-10
Identity = 129/584 (22.09%), Postives = 218/584 (37.33%), Query Frame = 0

Query: 25  AMALARCGVDRMFGVVG-IPVTSLATRAVSLGIRFIAFHNEQSAGYAASAYGYLTGRPG- 84
           A  L + GV  +F V G   +T L        +  I   NE +AGYAA  Y    G    
Sbjct: 52  ARRLVQAGVTDVFSVPGDFNLTLLDHLMAEPDLNLIGCCNELNAGYAADGYARSRGVGAC 111

Query: 85  -VLLTVSGPGCVHGLAGISNAMVNAWPLVMISGSCDQRDFGRG----------DF-QELD 144
            V  TV G   ++ +AG   A     PL+ I G  +  D+G            DF QEL 
Sbjct: 112 VVTFTVGGLSVLNAIAG---AYSENLPLICIVGGPNSNDYGTNRILHHTIGLPDFSQELR 171

Query: 145 QVEAVKPFSKISVKATDISE-IPNCVARVLNSAVSGRPGGCYFDLPSD---VLHQTISES 204
             + V  +  +     D  E I   ++  L  +   +P   Y  +  +   + H T S  
Sbjct: 172 CFQTVTCYQAVVNNLDDAHEQIDKAISTALKES---KP--VYISVSCNLAAIPHHTFSRD 231

Query: 205 EAERLLAAAEEFANREVIPRVPN-----SQIEEAISLLKHAERPLIVFGKGAAIARAEGP 264
                LA           PR+ N     + +E  +  L  A +P++V G    +A+A   
Sbjct: 232 PVPFSLA-----------PRLSNKMGLEAAVEATLEFLNKAVKPVMVGGPKLRVAKACDA 291

Query: 265 LKKLVETTGIPFLPTPMGKGLLPDTHE--------LAATAARSLAIGKCDVALVVGARLN 324
             +L + +G      P  KG +P+ H           +T   S  +   D  +  G   N
Sbjct: 292 FVELADASGYALAMMPSAKGFVPEHHPHFIGTYWGAVSTPFCSEIVESADAYIFAGPIFN 351

Query: 325 WLLHFGEPPKWSKDVKFILVDICKEEVELRKPHLGLIGDAKEVLESINKEIKDDPFCLGK 384
                G      K+ K I+V   +  V    P  G I    +    ++K +K        
Sbjct: 352 DYSSVGYSLLLKKE-KAIVVQPDRITV-ANGPTFGCI-LMSDFFRELSKRVKR------- 411

Query: 385 SHPWVGAISQKAKDNVAKMEVQLAKDVVPFNFLTPMRIIRDAILAVGSP----APILVSE 444
                   ++ A +N  ++ V   K         P++      L V +       +L SE
Sbjct: 412 --------NETAYENYHRIFVPEGK---------PLKCESREPLRVNTMFQHIQKMLSSE 471

Query: 445 GANTMDVGRSVLVQTEPRTRLDAG-----TWGTMGVGLGYCIAAAVASPDRLVVAVEGDS 504
            A   + G S     + +     G      +G++G  +G  +  A ASP++ V+A  GD 
Sbjct: 472 TAVIAETGDSWFNCQKLKLPKGCGYEFQMQYGSIGWSVGATLGYAQASPEKRVLAFIGDG 531

Query: 505 GFGFSAMEVETLVRYELPVVVIVFNNSGVYGGDRRTPQEITGPYKHDPAPTSFVPSASYD 564
            F  +  ++ T++R     ++ + NN G          EI     HD  P + + + +Y 
Sbjct: 532 SFQVTVQDISTMLRNGQKTIIFLINNGGY-----TIEVEI-----HD-GPYNVIKNWNYT 578

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LF462.8e-26580.212-hydroxyacyl-CoA lyase OS=Arabidopsis thaliana OX=3702 GN=HACL PE=1 SV=1[more]
Q0JMH01.5e-25576.112-hydroxyacyl-CoA lyase OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0505400... [more]
Q8CHM71.9e-12843.652-hydroxyacyl-CoA lyase 1 OS=Rattus norvegicus OX=10116 GN=Hacl1 PE=1 SV=1[more]
Q9UJ832.1e-12743.572-hydroxyacyl-CoA lyase 1 OS=Homo sapiens OX=9606 GN=HACL1 PE=1 SV=2[more]
P0AFI12.7e-12743.52Oxalyl-CoA decarboxylase OS=Escherichia coli O157:H7 OX=83334 GN=oxc PE=3 SV=1[more]
Match NameE-valueIdentityDescription
A0A5A7VDZ60.0e+00100.002-hydroxyacyl-CoA lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
A0A1S3B9H90.0e+00100.002-hydroxyacyl-CoA lyase OS=Cucumis melo OX=3656 GN=LOC103487508 PE=3 SV=1[more]
A0A6J1GAR50.0e+0093.772-hydroxyacyl-CoA lyase-like OS=Cucurbita moschata OX=3662 GN=LOC111452318 PE=3 ... [more]
A0A0A0KW040.0e+0095.50Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G133700 PE=3 SV=1[more]
A0A6J1KDD50.0e+0093.432-hydroxyacyl-CoA lyase OS=Cucurbita maxima OX=3661 GN=LOC111492838 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_008444073.10.0100.00PREDICTED: 2-hydroxyacyl-CoA lyase [Cucumis melo] >KAA0064125.1 2-hydroxyacyl-Co... [more]
XP_011653421.10.097.582-hydroxyacyl-CoA lyase [Cucumis sativus] >KAE8649340.1 hypothetical protein Csa... [more]
XP_038893948.10.096.372-hydroxyacyl-CoA lyase [Benincasa hispida][more]
XP_023524913.10.094.292-hydroxyacyl-CoA lyase-like [Cucurbita pepo subsp. pepo][more]
KAG7036762.10.093.942-hydroxyacyl-CoA lyase [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
AT5G17380.12.0e-26680.21Thiamine pyrophosphate dependent pyruvate decarboxylase family protein [more]
AT3G48560.11.3e-4428.69chlorsulfuron/imidazolinone resistant 1 [more]
AT4G33070.14.8e-1022.09Thiamine pyrophosphate dependent pyruvate decarboxylase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012001Thiamine pyrophosphate enzyme, N-terminal TPP-binding domainPFAMPF02776TPP_enzyme_Ncoord: 25..185
e-value: 1.1E-40
score: 139.1
IPR012000Thiamine pyrophosphate enzyme, central domainPFAMPF00205TPP_enzyme_Mcoord: 212..339
e-value: 9.4E-33
score: 112.9
NoneNo IPR availableGENE3D3.40.50.1220coord: 208..362
e-value: 4.8E-38
score: 132.3
NoneNo IPR availableGENE3D3.40.50.970coord: 364..578
e-value: 1.0E-63
score: 216.2
NoneNo IPR availableGENE3D3.40.50.970coord: 18..199
e-value: 2.1E-43
score: 149.8
NoneNo IPR availablePANTHERPTHR43710:SF22-HYDROXYACYL-COA LYASE 1coord: 18..578
NoneNo IPR availableCDDcd07035TPP_PYR_POX_likecoord: 27..175
e-value: 5.31504E-45
score: 154.612
NoneNo IPR availableCDDcd02004TPP_BZL_OCoD_HPCLcoord: 385..564
e-value: 4.58983E-81
score: 249.757
IPR011766Thiamine pyrophosphate enzyme, C-terminal TPP-bindingPFAMPF02775TPP_enzyme_Ccoord: 412..561
e-value: 5.8E-28
score: 97.6
IPR045025TPP-binding domain containing protein HACL1-likePANTHERPTHR437102-HYDROXYACYL-COA LYASEcoord: 18..578
IPR000399TPP-binding enzyme, conserved sitePROSITEPS00187TPP_ENZYMEScoord: 446..465
IPR029061Thiamin diphosphate-binding foldSUPERFAMILY52518Thiamin diphosphate-binding fold (THDP-binding)coord: 20..187
IPR029061Thiamin diphosphate-binding foldSUPERFAMILY52518Thiamin diphosphate-binding fold (THDP-binding)coord: 394..567
IPR029035DHS-like NAD/FAD-binding domain superfamilySUPERFAMILY52467DHS-like NAD/FAD-binding domaincoord: 207..365

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
IVF0025369.2IVF0025369.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0001561 fatty acid alpha-oxidation
cellular_component GO:0005777 peroxisome
molecular_function GO:0016829 lyase activity
molecular_function GO:0000287 magnesium ion binding
molecular_function GO:0030976 thiamine pyrophosphate binding
molecular_function GO:0003824 catalytic activity