IVF0025141 (gene) Melon (IVF77) v1

Overview


Name	IVF0025141
Type	gene
Organism	Cucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
Description	Cytoplasmic tRNA 2-thiolation protein 1
Location	chr12: 10196330 .. 10198874 (-)
RNA-Seq Expression	IVF0025141
Synteny	IVF0025141

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCTAATTGCGCTCACTCCATCACAGAATGCCTTGGTTTCTGATCGACTTCTTAGGCATTCAAATATTAATGTCAAAGTTGCAGTTGCAGCATGCGTTAGTGAAATAACTCGGATCATTGCACCTAATGCCCCTTACAGTGATGGAAATATGAAGGTAATCAGAAATTTGCTCGATTGAATGCCAATTTCCCACTTGTCGAATCTACTTCTAAAGAGTTGAATGTATGCTGCTGTTCAGGAGGTATTTCATCTTATAGTATCATCCTTTGAAAATCTCTCTGACAAGTCAAGTCGATCATATGTAAAATGCGCATCCATTCTTGAAACTGTTCCGAGGGTCAGATTATATGTGGCCATGCTGGATTTGGAATGTGATGCATTGATATGTTCCAACATTTCCTTAAGACAGTAAGGTATTATACTAGATTTTGTTTTGAAATAAAATTCCTTTTTAAATTTTTACTTATAAGACTATTTGTGGTAGCTATTAATTTTCCAACAATTCAAGTTCTTTTGTGAACAAATATAATTTGTTTCTCCATGAAATACTACTAGACTTCTGTTTTGAATCAGAACTTGACTTACTCCAGTATGACCATTATATTGGGTGTTTTGAATGAGATGTAGCAGTGGTATTTGAGTACATTTGAATGATTGTGTTAGACCCCCCAATATTACACACATATAAGAGAAGGGGCAGAAACGGGAATTAACATGGCTTATGCCAATTCGGGGAGCAAAGAGAGAGAAAGCATCTCCTGAGGGCCCACTGGGGAGTTGGTATTTATATAAAATGTCTTTGGGGATTGCTGTCATTAGGTTTTATGTTGTCTTTTTAGGTGAAGGCCGCCATAGCCTTCATTTGGGAGAAGTTTCCAGCCTTCTCTAAGGAGCTGGGTGTTAGTATTGTTTTTACTATTATTTTCTGGATTATAGCTCTGTTTTGTGGAACATTTTGAGTGTTATGATCAATATATTATAACAGAGTGCTGCCTCTGTTTTGTCTTGTTGGGACTTTTCTGCTTTTCAGGTTAAGAATCTTAACAAATTATCAAGAAAAACTCGGTTAAAAATATAATGCAGTGAATTAGTTGACTGTATGCATTGTAATAAGTTTGGCTTTGAACTATTGTCTGTTAAATAGGTTTTATTGGTTGAATGTGAATCCTTGTTACACTACAAGAAATTCTGGATCTCCTGACGTTGGTTTACACCGTCGGGAGATAAGGCATCTTCTGACGCAACAATTAATGAGTTGGGAGATGCAATATCCCCTGATGCCTTCATGCACGGCCTCGGAGATAGAAGAGAACTCCCGACGCCGTTAGTAATGCATCGGAGAACTGCTGACACTTTAATTAAAACGTTGACAGTTTTGGGATAACTCTCGACACTGTGTCGGGCTTTGTCGGAAGTTGGTCTTTAAAAACCCTCCTAGATTTGTATTTCACAGATCAGTGAAGAAGGGGAAGGGGAAATGAGAGACTCAGCTGAAAACCACCACCGTCGCTCGTGGCATCGCCCCGCCGCCGTTCTCGTCGTTTGCCCCGTCTCTTCTGGTGCCGCTGCTGTCCTCCTGTGTCTTTCTTGCTGCTGCTGCCCCTTTCTCTTCCGATATGTAACTTATTTAAGTTAGATTTAAATTTTGCCCTAAAATCCTAATTTGTTCAAAAACAATTGATTTCTAAAGGATTATGAGAGATGGTTTTTCAAAATCTATTGATTTCATAAGAGAGTAAAAAAGTGGTTATTAGCCATAATCTATTGATAGCCTTCTTCACTGTTTTTTGTTAATTCTCCCTTTGAGTTTTATTGGCCGTCTCTTATCCAAAGAATATTCTTCTCTATTAAGGCTTGTGGAAGCTTCCCTATCATTGTTTATCTCCTTCATTTTGTTCTGTTTGATTGGTGGTTATTGAAGGGACTCAATATCTTTTCTCTGAATGCTGATTTTTCAAGGTTTCAATGTATTTTCAAGACAAGCGTTGGCCTTAGCTTATTTGATTTGAAGTTTCCAGAGATAAAGCTTCTTTTTGTTGGTGTCTCTCTCCATTCTATTTGCAGAATTTTGTGACTTATGCTTAACCAGGATTGTATCTATTCTAGTTTAGGTATCTTGTATTGATCTATGTATTACTATAAGCATACTTGGCAAGTTCTCTTTTTGGTTCTTTGCGTATTAAGCATGGTTTCATTGTATTGATGGAAAATTTTCTCCTGAAGGCTCTTGATCGAGGTGCTGCATTGTTGAAAGTAGTCAAGCTTGCCACAAGGCACAATGCAATTGATATGGCTGAAACAGTTCTATTAGATATATTACGAGGTGATATTGCCAGGTATTTTAATAGTAAGACTTTTTTTCTGTCAATTACTTTTGTCCCTCATTTGGGCTGTATTAGATTGAAGTGATGCTTTGAATTTGTAGATTGAGTAGATGTACTGCAATAAGTACCGATGAAGATGGACCAATTCCAAGATGCAAACCTTTCAAGTACACATATGAAAAGAAGATAGTAGTATATTTTTATATTTTAGTCTCATAG

mRNA sequence

ATGCTAATTGCGCTCACTCCATCACAGAATGCCTTGGTTTCTGATCGACTTCTTAGGCATTCAAATATTAATGTCAAAGTTGCAGTTGCAGCATGCGTTAGTGAAATAACTCGGATCATTGCACCTAATGCCCCTTACAGTGATGGAAATATGAAGGAGGCTCTTGATCGAGGTGCTGCATTGTTGAAAGTAGTCAAGCTTGCCACAAGGCACAATGCAATTGATATGGCTGAAACAGTTCTATTAGATATATTACGAGGTGATATTGCCAGATTGAGTAGATGTACTGCAATAAGTACCGATGAAGATGGACCAATTCCAAGATGCAAACCTTTCAAGTACACATATGAAAAGAAGATAGTAGTATATTTTTATATTTTAGTCTCATAG

Coding sequence (CDS)

ATGCTAATTGCGCTCACTCCATCACAGAATGCCTTGGTTTCTGATCGACTTCTTAGGCATTCAAATATTAATGTCAAAGTTGCAGTTGCAGCATGCGTTAGTGAAATAACTCGGATCATTGCACCTAATGCCCCTTACAGTGATGGAAATATGAAGGAGGCTCTTGATCGAGGTGCTGCATTGTTGAAAGTAGTCAAGCTTGCCACAAGGCACAATGCAATTGATATGGCTGAAACAGTTCTATTAGATATATTACGAGGTGATATTGCCAGATTGAGTAGATGTACTGCAATAAGTACCGATGAAGATGGACCAATTCCAAGATGCAAACCTTTCAAGTACACATATGAAAAGAAGATAGTAGTATATTTTTATATTTTAGTCTCATAG

Protein sequence

MLIALTPSQNALVSDRLLRHSNINVKVAVAACVSEITRIIAPNAPYSDGNMKEALDRGAALLKVVKLATRHNAIDMAETVLLDILRGDIARLSRCTAISTDEDGPIPRCKPFKYTYEKKIVVYFYILVS

Homology

[Close]

BLAST of IVF0025141 vs. ExPASy Swiss-Prot
Match: O64862 (Cytoplasmic tRNA 2-thiolation protein 1 OS=Arabidopsis thaliana OX=3702 GN=NCS6 PE=2 SV=2)

HSP 1 Score: 124.4 bits (311), Expect = 9.3e-28
Identity = 64/95 (67.37%), Postives = 76/95 (80.00%), Query Frame = 0


Query: 33  VSEITRIIA--PNAPYSDGNMKEALDRGAALLKVVKLATRHNAIDMAETVLLDILRGDIA 92
           + EI ++I    N  +     ++ALDRGAALLKV KL T HNA D+AETVLL+ILRGDIA
Sbjct: 136 MDEIVKMIGLKNNCTFCGVFRRQALDRGAALLKVEKLVTGHNADDIAETVLLNILRGDIA 195

Query: 93  RLSRCTAISTDEDGPIPRCKPFKYTYEKKIVVYFY 126
           RLSRCT+I+T EDGPIPRCKPFKYTYEK+IV+Y Y
Sbjct: 196 RLSRCTSITTGEDGPIPRCKPFKYTYEKEIVMYAY 230

[Close]

BLAST of IVF0025141 vs. ExPASy Swiss-Prot
Match: Q6Z6G6 (Cytoplasmic tRNA 2-thiolation protein 1 OS=Oryza sativa subsp. japonica OX=39947 GN=NCS6 PE=2 SV=1)

HSP 1 Score: 121.3 bits (303), Expect = 7.9e-27
Identity = 59/74 (79.73%), Postives = 67/74 (90.54%), Query Frame = 0


Query: 52  KEALDRGAALLKVVKLATRHNAIDMAETVLLDILRGDIARLSRCTAISTDEDGPIPRCKP 111
           ++ALDRGAALLKV K+ T HNA D+AETVLL+ILRGDIARLSRCT I+T EDGPIPRCKP
Sbjct: 163 RQALDRGAALLKVDKIVTGHNADDIAETVLLNILRGDIARLSRCTFITTGEDGPIPRCKP 222

Query: 112 FKYTYEKKIVVYFY 126
           FKYTYEK+IV+Y Y
Sbjct: 223 FKYTYEKEIVMYAY 236

[Close]

BLAST of IVF0025141 vs. ExPASy Swiss-Prot
Match: Q05AW7 (Cytoplasmic tRNA 2-thiolation protein 1 OS=Xenopus laevis OX=8355 GN=ctu1 PE=2 SV=1)

HSP 1 Score: 104.8 bits (260), Expect = 7.7e-22
Identity = 49/74 (66.22%), Postives = 61/74 (82.43%), Query Frame = 0


Query: 52  KEALDRGAALLKVVKLATRHNAIDMAETVLLDILRGDIARLSRCTAISTDEDGPIPRCKP 111
           ++ALDRGA +L + K+ T HNA D+AETVL++ LRGDIARL RCTAI+T  +G IPRCKP
Sbjct: 148 RQALDRGAMMLGINKICTGHNADDIAETVLMNFLRGDIARLRRCTAITTGSEGAIPRCKP 207

Query: 112 FKYTYEKKIVVYFY 126
            KYTYEK+IV+Y Y
Sbjct: 208 LKYTYEKEIVLYAY 221

[Close]

BLAST of IVF0025141 vs. ExPASy Swiss-Prot
Match: Q803X1 (Cytoplasmic tRNA 2-thiolation protein 1 OS=Danio rerio OX=7955 GN=ctu1 PE=2 SV=1)

HSP 1 Score: 103.2 bits (256), Expect = 2.2e-21
Identity = 51/76 (67.11%), Postives = 62/76 (81.58%), Query Frame = 0


Query: 52  KEALDRGAALLKVVKLATRHNAIDMAETVLLDILRGDIARLSRCTAIST--DEDGPIPRC 111
           ++ALDRGA +L V K+ T HNA D+AETVL+++LRGDIARL RCTAIST  + DG IPRC
Sbjct: 148 RQALDRGAMMLNVDKICTGHNADDVAETVLMNVLRGDIARLRRCTAISTSSEGDGAIPRC 207

Query: 112 KPFKYTYEKKIVVYFY 126
           KP KY YEK+IV+Y Y
Sbjct: 208 KPLKYAYEKEIVLYAY 223

[Close]

BLAST of IVF0025141 vs. ExPASy Swiss-Prot
Match: Q5FW05 (Cytoplasmic tRNA 2-thiolation protein 1 OS=Xenopus tropicalis OX=8364 GN=ctu1 PE=2 SV=1)

HSP 1 Score: 102.1 bits (253), Expect = 5.0e-21
Identity = 48/74 (64.86%), Postives = 60/74 (81.08%), Query Frame = 0


Query: 52  KEALDRGAALLKVVKLATRHNAIDMAETVLLDILRGDIARLSRCTAISTDEDGPIPRCKP 111
           ++ALDRGA +L V K+ T HNA D+AETVL++ LRGDIARL RCT+I+T  +G IPRCKP
Sbjct: 148 RQALDRGAMMLGVNKICTGHNADDIAETVLMNFLRGDIARLRRCTSITTGSEGAIPRCKP 207

Query: 112 FKYTYEKKIVVYFY 126
            KY YEK+IV+Y Y
Sbjct: 208 LKYAYEKEIVLYAY 221

[Close]

BLAST of IVF0025141 vs. ExPASy TrEMBL
Match: A0A5D3DA09 (Cytoplasmic tRNA 2-thiolation protein 1 isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold708G00390 PE=4 SV=1)

HSP 1 Score: 188.0 bits (476), Expect = 2.5e-44
Identity = 122/247 (49.39%), Postives = 124/247 (50.20%), Query Frame = 0


Query: 1   MLIALTPSQNALVSDRLLRHSNINVKVAVAACVSEITRIIAPNAPYSDGNMKE------- 60
           MLIALTPSQNALVSDRLLRHSNINVKVAVAACVSEITRIIAPNAPYSDGNMKE       
Sbjct: 1   MLIALTPSQNALVSDRLLRHSNINVKVAVAACVSEITRIIAPNAPYSDGNMKEVFHLIVS 60

Query: 61  ------------------------------------------------------------ 120
                                                                       
Sbjct: 61  SFENLSDKSSRSYVKCASILETVPRVKAAIAFIWEKFPAFSKELGSAASVLSCWDFSAFQ 120

Query: 121 -------------------------------------------------------ALDRG 126
                                                                  ALDRG
Sbjct: 121 ISEEGEGEMRDSAENHHRRSWHRPAAVLVVCPVSSGAAAVLLCLSCCCCPFLFRYALDRG 180

[Close]

BLAST of IVF0025141 vs. ExPASy TrEMBL
Match: A0A5A7TI13 (Protein starmaker OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold125G001460 PE=4 SV=1)

HSP 1 Score: 167.5 bits (423), Expect = 3.6e-38
Identity = 112/234 (47.86%), Postives = 112/234 (47.86%), Query Frame = 0


Query: 1   MLIALTPSQNALVSDRLLRHSNINVKVAVAACVSEITRIIAPNAPYSDGNMKE------- 60
           MLIALTPSQNALVSDRLLRHSNINVKVAVAACVSEITRIIAPNAPYSDGNMKE       
Sbjct: 1   MLIALTPSQNALVSDRLLRHSNINVKVAVAACVSEITRIIAPNAPYSDGNMKEVFHLIVS 60

Query: 61  ------------------------------------------------------------ 113
                                                                       
Sbjct: 61  SFENLSDKSSRSYVKCASILETVPRVKAAIAFIWEKFPAFSKELGSAASVLSCWDFSAFQ 120

[Close]

BLAST of IVF0025141 vs. ExPASy TrEMBL
Match: A0A059BW08 (Cytoplasmic tRNA 2-thiolation protein 1 OS=Eucalyptus grandis OX=71139 GN=NCS6 PE=3 SV=1)

HSP 1 Score: 127.9 bits (320), Expect = 3.1e-26
Identity = 67/95 (70.53%), Postives = 77/95 (81.05%), Query Frame = 0


Query: 33  VSEITRIIA--PNAPYSDGNMKEALDRGAALLKVVKLATRHNAIDMAETVLLDILRGDIA 92
           + EI +II    N  +     ++ALDRGAALLKV KLAT HNA D+AETVLL+ILRGDIA
Sbjct: 139 MDEIVKIIGLKNNCTFCGVFRRQALDRGAALLKVDKLATGHNADDIAETVLLNILRGDIA 198

Query: 93  RLSRCTAISTDEDGPIPRCKPFKYTYEKKIVVYFY 126
           RLSRCTAI+T EDGPIPRCKPFKYTYEK+IV+Y Y
Sbjct: 199 RLSRCTAITTGEDGPIPRCKPFKYTYEKEIVMYAY 233

[Close]

BLAST of IVF0025141 vs. ExPASy TrEMBL
Match: A0A6J1KNU4 (Cytoplasmic tRNA 2-thiolation protein 1 OS=Cucurbita maxima OX=3661 GN=LOC111496254 PE=3 SV=1)

HSP 1 Score: 127.1 bits (318), Expect = 5.3e-26
Identity = 67/95 (70.53%), Postives = 76/95 (80.00%), Query Frame = 0


Query: 33  VSEITRIIA--PNAPYSDGNMKEALDRGAALLKVVKLATRHNAIDMAETVLLDILRGDIA 92
           + EI ++I    N  +     ++ALDRGAALLKV KLAT HNA DMAETVLL+ILRGDIA
Sbjct: 138 MDEIVKMIGLKNNCTFCGVFRRQALDRGAALLKVDKLATGHNADDMAETVLLNILRGDIA 197

Query: 93  RLSRCTAISTDEDGPIPRCKPFKYTYEKKIVVYFY 126
           RLSRCTAI T EDGPIPRCKPFKYTYEK+IV+Y Y
Sbjct: 198 RLSRCTAIITGEDGPIPRCKPFKYTYEKEIVMYAY 232

[Close]

BLAST of IVF0025141 vs. ExPASy TrEMBL
Match: A0A0A0LKF0 (Cytoplasmic tRNA 2-thiolation protein 1 OS=Cucumis sativus OX=3659 GN=NCS6 PE=3 SV=1)

HSP 1 Score: 127.1 bits (318), Expect = 5.3e-26
Identity = 67/95 (70.53%), Postives = 76/95 (80.00%), Query Frame = 0


Query: 33  VSEITRIIA--PNAPYSDGNMKEALDRGAALLKVVKLATRHNAIDMAETVLLDILRGDIA 92
           + EI ++I    N  +     ++ALDRGAALLKV KLAT HNA DMAETVLL+ILRGDIA
Sbjct: 138 MDEIVKMIGLKNNCTFCGVFRRQALDRGAALLKVDKLATGHNADDMAETVLLNILRGDIA 197

Query: 93  RLSRCTAISTDEDGPIPRCKPFKYTYEKKIVVYFY 126
           RLSRCTAI T EDGPIPRCKPFKYTYEK+IV+Y Y
Sbjct: 198 RLSRCTAIITGEDGPIPRCKPFKYTYEKEIVMYAY 232

[Close]

BLAST of IVF0025141 vs. NCBI nr
Match: TYK20310.1 (cytoplasmic tRNA 2-thiolation protein 1 isoform X2 [Cucumis melo var. makuwa])

HSP 1 Score: 188 bits (477), Expect = 7.51e-57
Identity = 122/247 (49.39%), Postives = 124/247 (50.20%), Query Frame = 0


Query: 1   MLIALTPSQNALVSDRLLRHSNINVKVAVAACVSEITRIIAPNAPYSDGNMKE------- 60
           MLIALTPSQNALVSDRLLRHSNINVKVAVAACVSEITRIIAPNAPYSDGNMKE       
Sbjct: 1   MLIALTPSQNALVSDRLLRHSNINVKVAVAACVSEITRIIAPNAPYSDGNMKEVFHLIVS 60

Query: 61  ------------------------------------------------------------ 120
                                                                       
Sbjct: 61  SFENLSDKSSRSYVKCASILETVPRVKAAIAFIWEKFPAFSKELGSAASVLSCWDFSAFQ 120

Query: 121 -------------------------------------------------------ALDRG 125
                                                                  ALDRG
Sbjct: 121 ISEEGEGEMRDSAENHHRRSWHRPAAVLVVCPVSSGAAAVLLCLSCCCCPFLFRYALDRG 180

[Close]

BLAST of IVF0025141 vs. NCBI nr
Match: KAA0040949.1 (protein starmaker [Cucumis melo var. makuwa])

HSP 1 Score: 167 bits (424), Expect = 2.12e-49
Identity = 112/234 (47.86%), Postives = 112/234 (47.86%), Query Frame = 0


Query: 1   MLIALTPSQNALVSDRLLRHSNINVKVAVAACVSEITRIIAPNAPYSDGNMKE------- 60
           MLIALTPSQNALVSDRLLRHSNINVKVAVAACVSEITRIIAPNAPYSDGNMKE       
Sbjct: 1   MLIALTPSQNALVSDRLLRHSNINVKVAVAACVSEITRIIAPNAPYSDGNMKEVFHLIVS 60

Query: 61  ------------------------------------------------------------ 112
                                                                       
Sbjct: 61  SFENLSDKSSRSYVKCASILETVPRVKAAIAFIWEKFPAFSKELGSAASVLSCWDFSAFQ 120

[Close]

BLAST of IVF0025141 vs. NCBI nr
Match: XP_030443958.1 (cytoplasmic tRNA 2-thiolation protein 1-like [Syzygium oleosum])

HSP 1 Score: 128 bits (321), Expect = 3.60e-34
Identity = 67/95 (70.53%), Postives = 77/95 (81.05%), Query Frame = 0


Query: 33  VSEITRIIA--PNAPYSDGNMKEALDRGAALLKVVKLATRHNAIDMAETVLLDILRGDIA 92
           + EI +II    N  +     ++ALDRGAALLKV KLAT HNA D+AETVLL+ILRGDIA
Sbjct: 25  MDEIVKIIGLKNNCTFCGVFRRQALDRGAALLKVDKLATGHNANDIAETVLLNILRGDIA 84

Query: 93  RLSRCTAISTDEDGPIPRCKPFKYTYEKKIVVYFY 125
           RLSRCTAI+T EDGPIPRCKPFKYTYEK+IV+Y Y
Sbjct: 85  RLSRCTAITTGEDGPIPRCKPFKYTYEKEIVMYAY 119

[Close]

BLAST of IVF0025141 vs. NCBI nr
Match: KAG7020429.1 (Cytoplasmic tRNA 2-thiolation protein 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 130 bits (328), Expect = 9.64e-34
Identity = 69/99 (69.70%), Postives = 80/99 (80.81%), Query Frame = 0


Query: 33  VSEITRIIA--PNAPYSDGNMKEALDRGAALLKVVKLATRHNAIDMAETVLLDILRGDIA 92
           + EI ++I    N  +     ++ALDRGAALLKV KLAT HNA DMAETVLL+ILRGDIA
Sbjct: 138 MDEIVKMIGLKNNCTFCGVFRRQALDRGAALLKVDKLATGHNADDMAETVLLNILRGDIA 197

Query: 93  RLSRCTAISTDEDGPIPRCKPFKYTYEKKIVVYFYILVS 129
           RLSRCTAI T EDGPIPRCKPFKYTYEK+IV+Y +I+VS
Sbjct: 198 RLSRCTAIITGEDGPIPRCKPFKYTYEKEIVIYPFIVVS 236

[Close]

BLAST of IVF0025141 vs. NCBI nr
Match: XP_031737415.1 (cytoplasmic tRNA 2-thiolation protein 1 isoform X2 [Cucumis sativus])

HSP 1 Score: 125 bits (313), Expect = 5.63e-33
Identity = 63/72 (87.50%), Postives = 66/72 (91.67%), Query Frame = 0


Query: 54  ALDRGAALLKVVKLATRHNAIDMAETVLLDILRGDIARLSRCTAISTDEDGPIPRCKPFK 113
           ALDRGAALLKV KLAT HNA DMAETVLL+ILRGDIARLSRCTAI T EDGPIPRCKPFK
Sbjct: 13  ALDRGAALLKVDKLATGHNADDMAETVLLNILRGDIARLSRCTAIITGEDGPIPRCKPFK 72

Query: 114 YTYEKKIVVYFY 125
           YTYEK+IV+Y Y
Sbjct: 73  YTYEKEIVMYAY 84

[Close]

BLAST of IVF0025141 vs. TAIR 10
Match: AT2G44270.1 (repressor of lrx1 )

HSP 1 Score: 124.4 bits (311), Expect = 6.6e-29
Identity = 64/95 (67.37%), Postives = 76/95 (80.00%), Query Frame = 0


Query: 33  VSEITRIIA--PNAPYSDGNMKEALDRGAALLKVVKLATRHNAIDMAETVLLDILRGDIA 92
           + EI ++I    N  +     ++ALDRGAALLKV KL T HNA D+AETVLL+ILRGDIA
Sbjct: 136 MDEIVKMIGLKNNCTFCGVFRRQALDRGAALLKVEKLVTGHNADDIAETVLLNILRGDIA 195

Query: 93  RLSRCTAISTDEDGPIPRCKPFKYTYEKKIVVYFY 126
           RLSRCT+I+T EDGPIPRCKPFKYTYEK+IV+Y Y
Sbjct: 196 RLSRCTSITTGEDGPIPRCKPFKYTYEKEIVMYAY 230

[Close]

BLAST of IVF0025141 vs. TAIR 10
Match: AT1G76170.1 (2-thiocytidine tRNA biosynthesis protein, TtcA )

HSP 1 Score: 122.9 bits (307), Expect = 1.9e-28
Identity = 63/95 (66.32%), Postives = 76/95 (80.00%), Query Frame = 0


Query: 33  VSEITRIIA--PNAPYSDGNMKEALDRGAALLKVVKLATRHNAIDMAETVLLDILRGDIA 92
           + EI ++I    N  +     ++ALDRGAALLKV KL T HNA D+AETV+L+ILRGDIA
Sbjct: 114 MDEIVKMIGLKNNCTFCGVFRRQALDRGAALLKVDKLVTGHNADDIAETVVLNILRGDIA 173

Query: 93  RLSRCTAISTDEDGPIPRCKPFKYTYEKKIVVYFY 126
           RLSRCT+I+T EDGPIPRCKPFKYTYEK+IV+Y Y
Sbjct: 174 RLSRCTSITTGEDGPIPRCKPFKYTYEKEIVMYAY 208

[Close]

BLAST of IVF0025141 vs. TAIR 10
Match: AT2G44270.2 (repressor of lrx1 )

HSP 1 Score: 109.0 bits (271), Expect = 2.9e-24
Identity = 56/86 (65.12%), Postives = 67/86 (77.91%), Query Frame = 0


Query: 33  VSEITRIIA--PNAPYSDGNMKEALDRGAALLKVVKLATRHNAIDMAETVLLDILRGDIA 92
           + EI ++I    N  +     ++ALDRGAALLKV KL T HNA D+AETVLL+ILRGDIA
Sbjct: 146 MDEIVKMIGLKNNCTFCGVFRRQALDRGAALLKVEKLVTGHNADDIAETVLLNILRGDIA 205

Query: 93  RLSRCTAISTDEDGPIPRCKPFKYTY 117
           RLSRCT+I+T EDGPIPRCKPF +TY
Sbjct: 206 RLSRCTSITTGEDGPIPRCKPFNFTY 231

[Close]

BLAST of IVF0025141 vs. TAIR 10
Match: AT4G31880.1 (LOCATED IN: cytosol, chloroplast; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 14 growth stages; BEST Arabidopsis thaliana protein match is: Tudor/PWWP/MBT superfamily protein (TAIR:AT1G15940.1); Has 137162 Blast hits to 70781 proteins in 2973 species: Archae - 289; Bacteria - 24182; Metazoa - 56725; Fungi - 20130; Plants - 6559; Viruses - 758; Other Eukaryotes - 28519 (source: NCBI BLink). )

HSP 1 Score: 68.6 bits (166), Expect = 4.3e-12
Identity = 32/50 (64.00%), Postives = 39/50 (78.00%), Query Frame = 0


Query: 4   ALTPSQNALVSDRLLRHSNINVKVAVAACVSEITRIIAPNAPYSDGNMKE 54
           ALTP    LV  +L +HS+++VKVAVAAC+SEITRI AP+APY D  MKE
Sbjct: 54  ALTPLMKGLVGGKLFKHSDVDVKVAVAACISEITRITAPDAPYDDDQMKE 103

[Close]

BLAST of IVF0025141 vs. TAIR 10
Match: AT4G31880.2 (LOCATED IN: cytosol; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 14 growth stages; BEST Arabidopsis thaliana protein match is: Tudor/PWWP/MBT superfamily protein (TAIR:AT1G15940.1). )

HSP 1 Score: 68.6 bits (166), Expect = 4.3e-12
Identity = 32/50 (64.00%), Postives = 39/50 (78.00%), Query Frame = 0


Query: 4   ALTPSQNALVSDRLLRHSNINVKVAVAACVSEITRIIAPNAPYSDGNMKE 54
           ALTP    LV  +L +HS+++VKVAVAAC+SEITRI AP+APY D  MKE
Sbjct: 54  ALTPLMKGLVGGKLFKHSDVDVKVAVAACISEITRITAPDAPYDDDQMKE 103

The following BLAST results are available for this feature:

BLAST of IVF0025141 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O64862	9.3e-28	67.37	Cytoplasmic tRNA 2-thiolation protein 1 OS=Arabidopsis thaliana OX=3702 GN=NCS6 ...	[more]
Q6Z6G6	7.9e-27	79.73	Cytoplasmic tRNA 2-thiolation protein 1 OS=Oryza sativa subsp. japonica OX=39947...	[more]
Q05AW7	7.7e-22	66.22	Cytoplasmic tRNA 2-thiolation protein 1 OS=Xenopus laevis OX=8355 GN=ctu1 PE=2 S...	[more]
Q803X1	2.2e-21	67.11	Cytoplasmic tRNA 2-thiolation protein 1 OS=Danio rerio OX=7955 GN=ctu1 PE=2 SV=1	[more]
Q5FW05	5.0e-21	64.86	Cytoplasmic tRNA 2-thiolation protein 1 OS=Xenopus tropicalis OX=8364 GN=ctu1 PE...	[more]

BLAST of IVF0025141 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3DA09	2.5e-44	49.39	Cytoplasmic tRNA 2-thiolation protein 1 isoform X2 OS=Cucumis melo var. makuwa O...	[more]
A0A5A7TI13	3.6e-38	47.86	Protein starmaker OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold125G00...	[more]
A0A059BW08	3.1e-26	70.53	Cytoplasmic tRNA 2-thiolation protein 1 OS=Eucalyptus grandis OX=71139 GN=NCS6 P...	[more]
A0A6J1KNU4	5.3e-26	70.53	Cytoplasmic tRNA 2-thiolation protein 1 OS=Cucurbita maxima OX=3661 GN=LOC111496...	[more]
A0A0A0LKF0	5.3e-26	70.53	Cytoplasmic tRNA 2-thiolation protein 1 OS=Cucumis sativus OX=3659 GN=NCS6 PE=3 ...	[more]

BLAST of IVF0025141 vs. NCBI nr


Match Name	E-value	Identity	Description
TYK20310.1	7.51e-57	49.39	cytoplasmic tRNA 2-thiolation protein 1 isoform X2 [Cucumis melo var. makuwa]	[more]
KAA0040949.1	2.12e-49	47.86	protein starmaker [Cucumis melo var. makuwa]	[more]
XP_030443958.1	3.60e-34	70.53	cytoplasmic tRNA 2-thiolation protein 1-like [Syzygium oleosum]	[more]
KAG7020429.1	9.64e-34	69.70	Cytoplasmic tRNA 2-thiolation protein 1 [Cucurbita argyrosperma subsp. argyrospe...	[more]
XP_031737415.1	5.63e-33	87.50	cytoplasmic tRNA 2-thiolation protein 1 isoform X2 [Cucumis sativus]	[more]

BLAST of IVF0025141 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G44270.1	6.6e-29	67.37	repressor of lrx1	[more]
AT1G76170.1	1.9e-28	66.32	2-thiocytidine tRNA biosynthesis protein, TtcA	[more]
AT2G44270.2	2.9e-24	65.12	repressor of lrx1	[more]
AT4G31880.1	4.3e-12	64.00	LOCATED IN: cytosol, chloroplast; EXPRESSED IN: 24 plant structures; EXPRESSED D...	[more]
AT4G31880.2	4.3e-12	64.00	LOCATED IN: cytosol; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 14 gro...	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR014729	Rossmann-like alpha/beta/alpha sandwich fold	GENE3D	3.40.50.620	HUPs	coord: 16..127 e-value: 2.9E-14 score: 54.8
None	No IPR available	PANTHER	PTHR11807	ATPASES OF THE PP SUPERFAMILY-RELATED	coord: 52..125
None	No IPR available	SUPERFAMILY	52402	Adenine nucleotide alpha hydrolases-like	coord: 53..124
IPR000541	Cytoplasmic tRNA 2-thiolation protein 1	PANTHER	PTHR11807:SF12	CYTOPLASMIC TRNA 2-THIOLATION PROTEIN 1	coord: 52..125

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
IVF0025141.1	IVF0025141.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032447	protein urmylation
biological_process	GO:0002143	tRNA wobble position uridine thiolation
biological_process	GO:0034227	tRNA thio-modification
biological_process	GO:0002098	tRNA wobble uridine modification
cellular_component	GO:0002144	cytosolic tRNA wobble base thiouridylase complex
molecular_function	GO:0016779	nucleotidyltransferase activity
molecular_function	GO:0000049	tRNA binding