IVF0025015 (gene) Melon (IVF77) v1

Overview


Name	IVF0025015
Type	gene
Organism	Cucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
Description	Protein kinase APK1B
Location	chr10: 4019854 .. 4021293 (+)
RNA-Seq Expression	IVF0025015
Synteny	IVF0025015

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGGATTCTGAAAATGGGAATGTCTTAATCTTTACATTTGAGGATTTGAAATCATTTACCAACAATTTCAATGGCAACAATCTGATCGGAATCACTCAGTCCGGCAGATTTTACAGAGGGCAGCTCCGACCACCGTGGTTGGCTGCAACTATTACAGAGACTCGAATTGTGACGGTGAAATTATGGGATCAAAAGCAAGAATTGGACAAAGAAAAAGTCAACCAAGTGTATTTTCAAGAAGTGAAGTTAATAACACACCAATCTCTAAAAAACAATCCAAGTTTGGCGAAATTGATTGGTTATTGCTGCGAAGATGGAGTTAAAGGTTTCGTTTATGATCTGAATCCATTGGGTTCGTTGCAAAATCTTAGGGGAAGAGGTAATTTACTACACATTGATTGCCATAAAACCAATGTCTTTGATGTGATTTTCGTCAAATGGACGATTCAAAATCTTATTGAAAAAGAAAATCAAGAGTAATTTTGTAGCTTTTAACCAAAGATGATGTAAGCAAGAGTTGGTAAAGATTATTTATTATCACTAATTTGAAGATATAAATATTCCTTTTCTTTTTTGTAGATGATCATATCAATTGGCTCCAAAGAGTTGATGTAATTCTCGAACTTGCTCGACTATTGGACTTCATTCACAATCAAGAGAACCAAAGTCTTGGCTTCCATTTTAGAGCATCAAATATACTGCTTGATTGGGTTTAGTTGTCTTCTTATCTTTAAACAATGAAGAGTTCAAAGCTACTGTTATCTCATGAATCTTCAACATTGCTAATCAGTTTATTCCAACAGGAATGCAAGCCGAAACTATGCGGATTCAAGCTGATTAGCGACAACGGGTTGGATGCTGACCTCTATAAACTTTCCAAGTTTACAAAGCTTCCTTTCGGCTATTTTCATCCCTCTATAAGAGGTACTGAATTACGTGTAAATACGAGAGTCAATGCGCTAGTTAAATAGACTCAAAACATCAAAACATAATATAAAAATTAAGTTTTGTTGCTTGAAATTTAACGAGTGAAGCTTTGCAGGTGAGAGTGAAGGAAAAGTGAGCTCTGATGTATATTCCTTGGGGGAAATTCTGCTGGGACTTATAGCCAAAAGAGATGTTGAGCCTCAGAGTTTGGGAAAACAAAATCGTCAAGAAGTTGTAAACAGCTCAGTGAGCATTTGGGCAAAGAATGAATATAGACCAAATGTTTCACTTGTTCATGAAAGCTTGCAAAAAGACTGGGGTTACAGCACGGAAGAGGGGATCAAACTCACGGAGCTGGGAATGCACAGTATAGAATTCTTTCCAAGAAATCGTCCGAGCATAAAGCAAATACTTCAGCATCTCGAAGCGTTGCAAGTTACTCAACGTCTTTCTGATGTCAGACCTAGAAAAAAGGAAAGAAAACTTCCTAGCAGCTCCATGGGAATGTAA

mRNA sequence

ATGGCGGATTCTGAAAATGGGAATGTCTTAATCTTTACATTTGAGGATTTGAAATCATTTACCAACAATTTCAATGGCAACAATCTGATCGGAATCACTCAGTCCGGCAGATTTTACAGAGGGCAGCTCCGACCACCGTGGTTGGCTGCAACTATTACAGAGACTCGAATTGTGACGGTGAAATTATGGGATCAAAAGCAAGAATTGGACAAAGAAAAAGTCAACCAAGTGTATTTTCAAGAAGTGAAGTTAATAACACACCAATCTCTAAAAAACAATCCAAGTTTGGCGAAATTGATTGGTTATTGCTGCGAAGATGGAGTTAAAGGTTTCGTTTATGATCTGAATCCATTGGGTTCGTTGCAAAATCTTAGGGGAAGAGATGATCATATCAATTGGCTCCAAAGAGTTGATGTAATTCTCGAACTTGCTCGACTATTGGACTTCATTCACAATCAAGAGAACCAAAGTCTTGGCTTCCATTTTAGAGCATCAAATATACTGCTTGATTGGGAATGCAAGCCGAAACTATGCGGATTCAAGCTGATTAGCGACAACGGGTTGGATGCTGACCTCTATAAACTTTCCAAGTTTACAAAGCTTCCTTTCGGCTATTTTCATCCCTCTATAAGAGGTGAGAGTGAAGGAAAAGTGAGCTCTGATGTATATTCCTTGGGGGAAATTCTGCTGGGACTTATAGCCAAAAGAGATGTTGAGCCTCAGAGTTTGGGAAAACAAAATCGTCAAGAAGTTGTAAACAGCTCAGTGAGCATTTGGGCAAAGAATGAATATAGACCAAATGTTTCACTTGTTCATGAAAGCTTGCAAAAAGACTGGGGTTACAGCACGGAAGAGGGGATCAAACTCACGGAGCTGGGAATGCACAGTATAGAATTCTTTCCAAGAAATCGTCCGAGCATAAAGCAAATACTTCAGCATCTCGAAGCGTTGCAAGTTACTCAACGTCTTTCTGATGTCAGACCTAGAAAAAAGGAAAGAAAACTTCCTAGCAGCTCCATGGGAATGTAA

Coding sequence (CDS)

ATGGCGGATTCTGAAAATGGGAATGTCTTAATCTTTACATTTGAGGATTTGAAATCATTTACCAACAATTTCAATGGCAACAATCTGATCGGAATCACTCAGTCCGGCAGATTTTACAGAGGGCAGCTCCGACCACCGTGGTTGGCTGCAACTATTACAGAGACTCGAATTGTGACGGTGAAATTATGGGATCAAAAGCAAGAATTGGACAAAGAAAAAGTCAACCAAGTGTATTTTCAAGAAGTGAAGTTAATAACACACCAATCTCTAAAAAACAATCCAAGTTTGGCGAAATTGATTGGTTATTGCTGCGAAGATGGAGTTAAAGGTTTCGTTTATGATCTGAATCCATTGGGTTCGTTGCAAAATCTTAGGGGAAGAGATGATCATATCAATTGGCTCCAAAGAGTTGATGTAATTCTCGAACTTGCTCGACTATTGGACTTCATTCACAATCAAGAGAACCAAAGTCTTGGCTTCCATTTTAGAGCATCAAATATACTGCTTGATTGGGAATGCAAGCCGAAACTATGCGGATTCAAGCTGATTAGCGACAACGGGTTGGATGCTGACCTCTATAAACTTTCCAAGTTTACAAAGCTTCCTTTCGGCTATTTTCATCCCTCTATAAGAGGTGAGAGTGAAGGAAAAGTGAGCTCTGATGTATATTCCTTGGGGGAAATTCTGCTGGGACTTATAGCCAAAAGAGATGTTGAGCCTCAGAGTTTGGGAAAACAAAATCGTCAAGAAGTTGTAAACAGCTCAGTGAGCATTTGGGCAAAGAATGAATATAGACCAAATGTTTCACTTGTTCATGAAAGCTTGCAAAAAGACTGGGGTTACAGCACGGAAGAGGGGATCAAACTCACGGAGCTGGGAATGCACAGTATAGAATTCTTTCCAAGAAATCGTCCGAGCATAAAGCAAATACTTCAGCATCTCGAAGCGTTGCAAGTTACTCAACGTCTTTCTGATGTCAGACCTAGAAAAAAGGAAAGAAAACTTCCTAGCAGCTCCATGGGAATGTAA

Protein sequence

MADSENGNVLIFTFEDLKSFTNNFNGNNLIGITQSGRFYRGQLRPPWLAATITETRIVTVKLWDQKQELDKEKVNQVYFQEVKLITHQSLKNNPSLAKLIGYCCEDGVKGFVYDLNPLGSLQNLRGRDDHINWLQRVDVILELARLLDFIHNQENQSLGFHFRASNILLDWECKPKLCGFKLISDNGLDADLYKLSKFTKLPFGYFHPSIRGESEGKVSSDVYSLGEILLGLIAKRDVEPQSLGKQNRQEVVNSSVSIWAKNEYRPNVSLVHESLQKDWGYSTEEGIKLTELGMHSIEFFPRNRPSIKQILQHLEALQVTQRLSDVRPRKKERKLPSSSMGM

Homology

[Close]

BLAST of IVF0025015 vs. ExPASy Swiss-Prot
Match: P46573 (Probable serine/threonine-protein kinase PBL10 OS=Arabidopsis thaliana OX=3702 GN=PBL10 PE=1 SV=2)

HSP 1 Score: 123.6 bits (309), Expect = 4.2e-27
Identity = 103/341 (30.21%), Postives = 162/341 (47.51%), Query Frame = 0


Query: 5   ENGNVLIFTFEDLKSFTNNFNGNNLIGITQSGRFYRGQLRPPWLAATITETRIVTVKLWD 64
           ++ N+  FTF +LK+ T NF  ++++G    G  ++G +    L A+   T +V      
Sbjct: 50  QSPNLKSFTFAELKAATRNFRPDSVLGEGGFGSVFKGWIDEQTLTASKPGTGVVIAV--- 109

Query: 65  QKQELDKEKVNQVYFQEVKLITHQSLKNNPSLAKLIGYCCEDGVKGFVYDLNPLGSLQN- 124
           +K   D  + +Q +  EV  +   S   +P+L KLIGYC ED  +  VY+  P GSL+N 
Sbjct: 110 KKLNQDGWQGHQEWLAEVNYLGQFS---HPNLVKLIGYCLEDEHRLLVYEFMPRGSLENH 169

Query: 125 LRGRDDH---INWLQRVDVILELARLLDFIHNQENQSLGFHFRASNILLDWECKPKLCGF 184
           L  R  +   ++W  R+ V L  A+ L F+HN E   +   F+ SNILLD E   KL  F
Sbjct: 170 LFRRGSYFQPLSWTLRLKVALGAAKGLAFLHNAETSVIYRDFKTSNILLDSEYNAKLSDF 229

Query: 185 KLISDNGLDADLYKLSKFTKLPFGYFHPSIRGESEGKVSSDVYSLGEILLGLIAKRDVEP 244
            L  D G   D   +S      +GY  P           SDVYS G +LL +++ R    
Sbjct: 230 GLAKD-GPTGDKSHVSTRIMGTYGYAAPEYLATGHLTTKSDVYSYGVVLLEVLSGRRAVD 289

Query: 245 QSLGKQNRQEVVNSSVSIWAKNEYRPNVSLVHESLQKDWGYSTEEGIKLTELGMHSIEFF 304
           ++      Q++V  +  + A    R    ++   LQ    YS EE  K+  L +  + F 
Sbjct: 290 KN-RPPGEQKLVEWARPLLANK--RKLFRVIDNRLQDQ--YSMEEACKVATLALRCLTFE 349

Query: 305 PRNRPSIKQILQHLEALQVTQ----RLSDVRPRKKERKLPS 338
            + RP++ +++ HLE +Q       R  D+  R+  R+  S
Sbjct: 350 IKLRPNMNEVVSHLEHIQTLNEAGGRNIDMVQRRMRRRSDS 378

[Close]

BLAST of IVF0025015 vs. ExPASy Swiss-Prot
Match: O48814 (Serine/threonine-protein kinase BIK1 OS=Arabidopsis thaliana OX=3702 GN=BIK1 PE=1 SV=1)

HSP 1 Score: 118.2 bits (295), Expect = 1.8e-25
Identity = 101/337 (29.97%), Postives = 159/337 (47.18%), Query Frame = 0


Query: 12  FTFEDLKSFTNNFNGNNLIGITQSGRFYRGQLRPPWLAATITETRIVTVKLWDQKQELDK 71
           FTF +LK  T NF  +++IG    G  ++G L    L  T   T +V          +  
Sbjct: 55  FTFNELKLATRNFRPDSVIGEGGFGCVFKGWLDESTLTPTKPGTGLV----------IAV 114

Query: 72  EKVNQVYFQE----VKLITHQSLKNNPSLAKLIGYCCEDGVKGFVYDLNPLGSLQNLRGR 131
           +K+NQ  FQ     +  I +    ++P+L KLIGYC ED  +  VY+    GSL+N   R
Sbjct: 115 KKLNQEGFQGHREWLTEINYLGQLSHPNLVKLIGYCLEDEHRLLVYEFMQKGSLENHLFR 174

Query: 132 D----DHINWLQRVDVILELARLLDFIHNQENQSLGFHFRASNILLDWECKPKLCGFKLI 191
                  + W  RV+V L+ A+ L F+H+   + +    +ASNILLD +   KL  F L 
Sbjct: 175 RGAYFKPLPWFLRVNVALDAAKGLAFLHSDPVKVIYRDIKASNILLDADYNAKLSDFGLA 234

Query: 192 SDNGLDADLYKLSKFTKLPFGYFHPSIRGESEGKVSSDVYSLGEILLGLIA-KRDVEPQS 251
            D G   DL  +S      +GY  P           SDVYS G +LL +++ KR ++   
Sbjct: 235 RD-GPMGDLSYVSTRVMGTYGYAAPEYMSSGHLNARSDVYSFGVLLLEILSGKRALDHNR 294

Query: 252 LGKQNRQEVVNSSVSIWAK---NEYRPNVSLVHESLQKDWGYSTEEGIKLTELGMHSIEF 311
             K+        ++  WA+      R  + +V   L  D  Y  EE +++  + +  + F
Sbjct: 295 PAKE-------ENLVDWARPYLTSKRKVLLIVDNRL--DTQYLPEEAVRMASVAVQCLSF 354

Query: 312 FPRNRPSIKQILQHLEALQVT-QRLSDVRPRKKERKL 336
            P++RP++ Q+++ L+ LQ    + S   P K  +KL
Sbjct: 355 EPKSRPTMDQVVRALQQLQDNLGKPSQTNPVKDTKKL 371

[Close]

BLAST of IVF0025015 vs. ExPASy Swiss-Prot
Match: P27450 (Probable serine/threonine-protein kinase CST OS=Arabidopsis thaliana OX=3702 GN=CST PE=1 SV=2)

HSP 1 Score: 116.7 bits (291), Expect = 5.2e-25
Identity = 96/336 (28.57%), Postives = 156/336 (46.43%), Query Frame = 0


Query: 5   ENGNVLIFTFEDLKSFTNNFNGNNLIGITQSGRFYRGQLRPPWLAATITETRIVTVKLWD 64
           E+ N+ ++ F DLK+ T NF  ++++G    G+ YRG     W+ AT      V   +  
Sbjct: 67  ESPNLKVYNFLDLKTATKNFKPDSMLGQGGFGKVYRG-----WVDATTLAPSRVGSGMIV 126

Query: 65  QKQELDKEKVNQVYFQEVKLITHQSLKNNPSLAKLIGYCCEDGVKGFVYDLNPLGSLQ-N 124
             + L+ E V Q + +    +    + ++ +L KL+GYC ED     VY+  P GSL+ +
Sbjct: 127 AIKRLNSESV-QGFAEWRSEVNFLGMLSHRNLVKLLGYCREDKELLLVYEFMPKGSLESH 186

Query: 125 LRGRDDHINWLQRVDVILELARLLDFIHNQENQSLGFHFRASNILLDWECKPKLCGFKLI 184
           L  R+D   W  R+ +++  AR L F+H+ + + +   F+ASNILLD     KL  F L 
Sbjct: 187 LFRRNDPFPWDLRIKIVIGAARGLAFLHSLQREVIYRDFKASNILLDSNYDAKLSDFGLA 246

Query: 185 SDNGLDADLYKLSKFTKLPFGYFHPSIRGESEGKVSSDVYSLG----EILLGLIAKRDVE 244
                D   +  ++     +GY  P         V SDV++ G    EI+ GL A     
Sbjct: 247 KLGPADEKSHVTTRIMG-TYGYAAPEYMATGHLYVKSDVFAFGVVLLEIMTGLTAHNTKR 306

Query: 245 PQSLGKQNRQEVVNSSVSIWAKNEYRPNVSLVHESLQ-KDWG----YSTEEGIKLTELGM 304
           P+             S+  W     RP +S  H   Q  D G    Y+T+   ++  + +
Sbjct: 307 PRG----------QESLVDW----LRPELSNKHRVKQIMDKGIKGQYTTKVATEMARITL 366

Query: 305 HSIEFFPRNRPSIK---QILQHLEALQVTQRLSDVR 328
             IE  P+NRP +K   ++L+H++ L V    S  +
Sbjct: 367 SCIEPDPKNRPHMKEVVEVLEHIQGLNVVPNRSSTK 381

[Close]

BLAST of IVF0025015 vs. ExPASy Swiss-Prot
Match: Q8H186 (Probable serine/threonine-protein kinase PBL1 OS=Arabidopsis thaliana OX=3702 GN=PBL1 PE=1 SV=1)

HSP 1 Score: 115.2 bits (287), Expect = 1.5e-24
Identity = 90/315 (28.57%), Postives = 149/315 (47.30%), Query Frame = 0


Query: 12  FTFEDLKSFTNNFNGNNLIGITQSGRFYRGQLRPPWLAATITETRIVTVKLWDQKQELDK 71
           F+F +LK  T NF  ++++G    G  +RG L    L  T + + +V      ++   D 
Sbjct: 49  FSFNELKLATRNFRSDSVVGEGGFGCVFRGWLDETTLTPTKSSSGLVIAV---KRLNPDG 108

Query: 72  EKVNQVYFQEVKLITHQSLKNNPSLAKLIGYCCEDGVKGFVYDLNPLGSLQN---LRGRD 131
            + ++ +  E+  +   S   +P+L KLIGYC ED  +  VY+    GSL+N     G  
Sbjct: 109 FQGHREWLTEINYLGQLS---HPNLVKLIGYCLEDEQRLLVYEFMHKGSLENHLFANGNK 168

Query: 132 DH--INWLQRVDVILELARLLDFIHNQENQSLGFHFRASNILLDWECKPKLCGFKLISDN 191
           D   ++W+ R+ V L+ A+ L F+H+   + +    +ASNILLD +   KL  F L  D 
Sbjct: 169 DFKPLSWILRIKVALDAAKGLAFLHSDPVKVIYRDIKASNILLDSDFNAKLSDFGLARDG 228

Query: 192 GLDADLYKLSKFTKLPFGYFHPSIRGESEGKVSSDVYSLGEILLGLIAKRDVEPQSLGKQ 251
            +    Y +S      FGY  P           SDVYS G +LL L+  R    Q+L   
Sbjct: 229 PMGEQSY-VSTRVMGTFGYAAPEYVSTGHLNARSDVYSFGVVLLELLCGR----QAL--D 288

Query: 252 NRQEVVNSSVSIWAK---NEYRPNVSLVHESLQKDWGYSTEEGIKLTELGMHSIEFFPRN 311
           + +     ++  WA+      R  + +V   L     Y  E  ++L  + +  + F P++
Sbjct: 289 HNRPAKEQNLVDWARPYLTSRRKVLLIVDTRLNSQ--YKPEGAVRLASIAVQCLSFEPKS 348

Query: 312 RPSIKQILQHLEALQ 319
           RP++ Q+++ L  LQ
Sbjct: 349 RPTMDQVVRALVQLQ 348

[Close]

BLAST of IVF0025015 vs. ExPASy Swiss-Prot
Match: Q06548 (Probable serine/threonine-protein kinase PBL9 OS=Arabidopsis thaliana OX=3702 GN=PBL9 PE=1 SV=1)

HSP 1 Score: 115.2 bits (287), Expect = 1.5e-24
Identity = 97/321 (30.22%), Postives = 148/321 (46.11%), Query Frame = 0


Query: 5   ENGNVLIFTFEDLKSFTNNFNGNNLIGITQSGRFYRGQLRPPWLAATITETRIVTVKLWD 64
           ++ N+  F+F +LKS T NF  ++++G    G  ++G +    L A+   T +V      
Sbjct: 49  QSPNLKSFSFAELKSATRNFRPDSVLGEGGFGCVFKGWIDEKSLTASRPGTGLVIAV--- 108

Query: 65  QKQELDKEKVNQVYFQEVKLITHQSLKNNPSLAKLIGYCCEDGVKGFVYDLNPLGSLQNL 124
           +K   D  + +Q +  EV  +   S ++   L KLIGYC ED  +  VY+  P GSL+N 
Sbjct: 109 KKLNQDGWQGHQEWLAEVNYLGQFSHRH---LVKLIGYCLEDEHRLLVYEFMPRGSLENH 168

Query: 125 RGRD----DHINWLQRVDVILELARLLDFIHNQENQSLGFHFRASNILLDWECKPKLCGF 184
             R       ++W  R+ V L  A+ L F+H+ E + +   F+ SNILLD E   KL  F
Sbjct: 169 LFRRGLYFQPLSWKLRLKVALGAAKGLAFLHSSETRVIYRDFKTSNILLDSEYNAKLSDF 228

Query: 185 KLISDNGLDADLYKLSKFTKLPFGYFHPSIRGESEGKVSSDVYSLGEILLGLIAKRDVEP 244
            L  D G   D   +S       GY  P           SDVYS G +LL L++ R    
Sbjct: 229 GLAKD-GPIGDKSHVSTRVMGTHGYAAPEYLATGHLTTKSDVYSFGVVLLELLSGRRAVD 288

Query: 245 QSLGKQNRQEVVNSSVSIWAKN---EYRPNVSLVHESLQKDWGYSTEEGIKLTELGMHSI 304
           ++     R  V       WAK      R    ++   LQ    YS EE  K+  L +  +
Sbjct: 289 KNRPSGERNLVE------WAKPYLVNKRKIFRVIDNRLQDQ--YSMEEACKVATLSLRCL 348

Query: 305 EFFPRNRPSIKQILQHLEALQ 319
               + RP++ +++ HLE +Q
Sbjct: 349 TTEIKLRPNMSEVVSHLEHIQ 354

[Close]

BLAST of IVF0025015 vs. ExPASy TrEMBL
Match: A0A5A7SW68 (Protein kinase APK1B OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G002340 PE=4 SV=1)

HSP 1 Score: 696.0 bits (1795), Expect = 7.6e-197
Identity = 342/342 (100.00%), Postives = 342/342 (100.00%), Query Frame = 0


Query: 1   MADSENGNVLIFTFEDLKSFTNNFNGNNLIGITQSGRFYRGQLRPPWLAATITETRIVTV 60
           MADSENGNVLIFTFEDLKSFTNNFNGNNLIGITQSGRFYRGQLRPPWLAATITETRIVTV
Sbjct: 15  MADSENGNVLIFTFEDLKSFTNNFNGNNLIGITQSGRFYRGQLRPPWLAATITETRIVTV 74

Query: 61  KLWDQKQELDKEKVNQVYFQEVKLITHQSLKNNPSLAKLIGYCCEDGVKGFVYDLNPLGS 120
           KLWDQKQELDKEKVNQVYFQEVKLITHQSLKNNPSLAKLIGYCCEDGVKGFVYDLNPLGS
Sbjct: 75  KLWDQKQELDKEKVNQVYFQEVKLITHQSLKNNPSLAKLIGYCCEDGVKGFVYDLNPLGS 134

Query: 121 LQNLRGRDDHINWLQRVDVILELARLLDFIHNQENQSLGFHFRASNILLDWECKPKLCGF 180
           LQNLRGRDDHINWLQRVDVILELARLLDFIHNQENQSLGFHFRASNILLDWECKPKLCGF
Sbjct: 135 LQNLRGRDDHINWLQRVDVILELARLLDFIHNQENQSLGFHFRASNILLDWECKPKLCGF 194

Query: 181 KLISDNGLDADLYKLSKFTKLPFGYFHPSIRGESEGKVSSDVYSLGEILLGLIAKRDVEP 240
           KLISDNGLDADLYKLSKFTKLPFGYFHPSIRGESEGKVSSDVYSLGEILLGLIAKRDVEP
Sbjct: 195 KLISDNGLDADLYKLSKFTKLPFGYFHPSIRGESEGKVSSDVYSLGEILLGLIAKRDVEP 254

Query: 241 QSLGKQNRQEVVNSSVSIWAKNEYRPNVSLVHESLQKDWGYSTEEGIKLTELGMHSIEFF 300
           QSLGKQNRQEVVNSSVSIWAKNEYRPNVSLVHESLQKDWGYSTEEGIKLTELGMHSIEFF
Sbjct: 255 QSLGKQNRQEVVNSSVSIWAKNEYRPNVSLVHESLQKDWGYSTEEGIKLTELGMHSIEFF 314

Query: 301 PRNRPSIKQILQHLEALQVTQRLSDVRPRKKERKLPSSSMGM 343
           PRNRPSIKQILQHLEALQVTQRLSDVRPRKKERKLPSSSMGM
Sbjct: 315 PRNRPSIKQILQHLEALQVTQRLSDVRPRKKERKLPSSSMGM 356

[Close]

BLAST of IVF0025015 vs. ExPASy TrEMBL
Match: A0A1S3BEW9 (protein kinase APK1B, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103488875 PE=4 SV=1)

HSP 1 Score: 696.0 bits (1795), Expect = 7.6e-197
Identity = 342/342 (100.00%), Postives = 342/342 (100.00%), Query Frame = 0


Query: 1   MADSENGNVLIFTFEDLKSFTNNFNGNNLIGITQSGRFYRGQLRPPWLAATITETRIVTV 60
           MADSENGNVLIFTFEDLKSFTNNFNGNNLIGITQSGRFYRGQLRPPWLAATITETRIVTV
Sbjct: 15  MADSENGNVLIFTFEDLKSFTNNFNGNNLIGITQSGRFYRGQLRPPWLAATITETRIVTV 74

Query: 61  KLWDQKQELDKEKVNQVYFQEVKLITHQSLKNNPSLAKLIGYCCEDGVKGFVYDLNPLGS 120
           KLWDQKQELDKEKVNQVYFQEVKLITHQSLKNNPSLAKLIGYCCEDGVKGFVYDLNPLGS
Sbjct: 75  KLWDQKQELDKEKVNQVYFQEVKLITHQSLKNNPSLAKLIGYCCEDGVKGFVYDLNPLGS 134

Query: 121 LQNLRGRDDHINWLQRVDVILELARLLDFIHNQENQSLGFHFRASNILLDWECKPKLCGF 180
           LQNLRGRDDHINWLQRVDVILELARLLDFIHNQENQSLGFHFRASNILLDWECKPKLCGF
Sbjct: 135 LQNLRGRDDHINWLQRVDVILELARLLDFIHNQENQSLGFHFRASNILLDWECKPKLCGF 194

Query: 181 KLISDNGLDADLYKLSKFTKLPFGYFHPSIRGESEGKVSSDVYSLGEILLGLIAKRDVEP 240
           KLISDNGLDADLYKLSKFTKLPFGYFHPSIRGESEGKVSSDVYSLGEILLGLIAKRDVEP
Sbjct: 195 KLISDNGLDADLYKLSKFTKLPFGYFHPSIRGESEGKVSSDVYSLGEILLGLIAKRDVEP 254

Query: 241 QSLGKQNRQEVVNSSVSIWAKNEYRPNVSLVHESLQKDWGYSTEEGIKLTELGMHSIEFF 300
           QSLGKQNRQEVVNSSVSIWAKNEYRPNVSLVHESLQKDWGYSTEEGIKLTELGMHSIEFF
Sbjct: 255 QSLGKQNRQEVVNSSVSIWAKNEYRPNVSLVHESLQKDWGYSTEEGIKLTELGMHSIEFF 314

Query: 301 PRNRPSIKQILQHLEALQVTQRLSDVRPRKKERKLPSSSMGM 343
           PRNRPSIKQILQHLEALQVTQRLSDVRPRKKERKLPSSSMGM
Sbjct: 315 PRNRPSIKQILQHLEALQVTQRLSDVRPRKKERKLPSSSMGM 356

[Close]

BLAST of IVF0025015 vs. ExPASy TrEMBL
Match: A0A0A0KPY2 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G589310 PE=4 SV=1)

HSP 1 Score: 628.6 bits (1620), Expect = 1.5e-176
Identity = 310/343 (90.38%), Postives = 325/343 (94.75%), Query Frame = 0


Query: 1   MADSENGNVLIFTFEDLKSFTNNFNGNNLIGITQSGRFYRGQLRPPWLAATITETRIVTV 60
           MAD ENGNVLIFTFEDLKSFTNNFNGNNLIGITQSGRFYRGQLRPPWLAA ITETRIVTV
Sbjct: 1   MADFENGNVLIFTFEDLKSFTNNFNGNNLIGITQSGRFYRGQLRPPWLAAIITETRIVTV 60

Query: 61  KLWDQKQELDKEKVNQVYFQ-EVKLITHQSLKNNPSLAKLIGYCCEDGVKGFVYDLNPLG 120
           K+WDQKQEL+KEKVNQ+YFQ E+KLITHQSL+NNPSLAKLIGYCCEDGVKGFVYDLNPLG
Sbjct: 61  KIWDQKQELEKEKVNQLYFQEELKLITHQSLRNNPSLAKLIGYCCEDGVKGFVYDLNPLG 120

Query: 121 SLQNLRGRDDHINWLQRVDVILELARLLDFIHNQENQSLGFHFRASNILLDWECKPKLCG 180
            LQNL  RDD INW QRVDVILELARLLDFIH+QENQSLGFHF ASNILLDW+CKPKLC 
Sbjct: 121 LLQNLMARDDRINWFQRVDVILELARLLDFIHSQENQSLGFHFSASNILLDWDCKPKLCR 180

Query: 181 FKLISDNGLDADLYKLSKFTKLPFGYFHPSIRGESEGKVSSDVYSLGEILLGLIAKRDVE 240
           FK ISD+ ++ADLYKLSKFT+LP GYFHPSIRGESEGKV+SDVYSLGEILLGLIAKRDVE
Sbjct: 181 FKPISDDRMNADLYKLSKFTQLPSGYFHPSIRGESEGKVNSDVYSLGEILLGLIAKRDVE 240

Query: 241 PQSLGKQNRQEVVNSSVSIWAKNEYRPNVSLVHESLQKDWGYSTEEGIKLTELGMHSIEF 300
           PQ+L KQN QE VNSSVSIWAKNEYRPNVSLVHESLQKDWGYSTEEGIKLTEL MHSIEF
Sbjct: 241 PQNLEKQNHQEFVNSSVSIWAKNEYRPNVSLVHESLQKDWGYSTEEGIKLTELAMHSIEF 300

Query: 301 FPRNRPSIKQILQHLEALQVTQRLSDVRPRKKERKLPSSSMGM 343
           FPRNRPSIKQILQHLEALQVT+RLSD RPRKKERKLPSSS+GM
Sbjct: 301 FPRNRPSIKQILQHLEALQVTRRLSDDRPRKKERKLPSSSVGM 343

[Close]

BLAST of IVF0025015 vs. ExPASy TrEMBL
Match: A0A6J1HNY6 (probable serine/threonine-protein kinase CST OS=Cucurbita maxima OX=3661 GN=LOC111466402 PE=4 SV=1)

HSP 1 Score: 470.3 bits (1209), Expect = 6.8e-129
Identity = 243/342 (71.05%), Postives = 277/342 (80.99%), Query Frame = 0


Query: 2   ADSENGNVLIFTFEDLKSFTNNFNGNNLIGITQSGRFYRGQLRPPWLAATITETRIVTVK 61
           ADS NGNVLIFT EDLK+ T+NF+GN+LIGITQS RFYRGQLR  W  +T TE R +TVK
Sbjct: 14  ADSANGNVLIFTSEDLKTLTSNFHGNSLIGITQSCRFYRGQLRAAW--STGTEARNLTVK 73

Query: 62  LWDQKQELDKEKVNQ-VYFQEVKLITHQSLKNNPSLAKLIGYCCEDGVKGFVYDLNPLGS 121
           +WDQK E D    N+ +  +EVKL TH SL  + SLAKLIGYCCED VKG VYD +PLGS
Sbjct: 74  IWDQKPEFDNAITNEFLVREEVKLSTHSSLNGHRSLAKLIGYCCEDEVKGVVYDFDPLGS 133

Query: 122 LQNLRGRDDHINWLQRVDVILELARLLDFIHNQENQSLGFHFRASNILLDWECKPKLCGF 181
           LQNL  RDD +NWLQRV+VILELARLLDFIH+QE QSL   FRASNILLDWECKPKLCG 
Sbjct: 134 LQNLVARDD-LNWLQRVEVILELARLLDFIHSQEKQSLPIKFRASNILLDWECKPKLCGL 193

Query: 182 KLISDNGLDADLYKLSKFTKLPFGYFHPSIRGESEGKVSSDVYSLGEILLGLIAKRDVEP 241
           ++IS NG  AD   L+ F + P  YFH S RG S+GK+S+D++SLGEILLGLIAKRD EP
Sbjct: 194 EVIS-NGAGAD---LNNFAQNPSSYFHLSTRGGSQGKMSTDIFSLGEILLGLIAKRDYEP 253

Query: 242 QSLGKQNRQEVVNSSVSIWAKNEYRPNVSLVHESLQKDWGYSTEEGIKLTELGMHSIEFF 301
           + L ++N   VVNS VS WAKNEYRPN SLVHE+LQKDWGY+ EEG++LTEL +HSIEFF
Sbjct: 254 EKLERENHHRVVNSLVSNWAKNEYRPNGSLVHETLQKDWGYTVEEGVELTELAIHSIEFF 313

Query: 302 PRNRPSIKQILQHLEALQVTQRLSDVRPRKKERKLPSSSMGM 343
           PRNRPSIKQILQ LE LQVTQRLSD RPRKKERK PSS+MG+
Sbjct: 314 PRNRPSIKQILQRLEGLQVTQRLSDTRPRKKERKSPSSAMGI 348

[Close]

BLAST of IVF0025015 vs. ExPASy TrEMBL
Match: A0A6J1G0E5 (serine/threonine-protein kinase BIK1-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111449550 PE=4 SV=1)

HSP 1 Score: 466.8 bits (1200), Expect = 7.5e-128
Identity = 242/342 (70.76%), Postives = 273/342 (79.82%), Query Frame = 0


Query: 2   ADSENGNVLIFTFEDLKSFTNNFNGNNLIGITQSGRFYRGQLRPPWLAATITETRIVTVK 61
           ADS NGNVLIFT EDLK+ T+NF+ N+LIGITQSGRFYRGQLR  W  +T TE R +TVK
Sbjct: 14  ADSANGNVLIFTSEDLKTLTSNFHSNSLIGITQSGRFYRGQLRSAW--STGTEARNLTVK 73

Query: 62  LWDQKQELDKEKVNQ-VYFQEVKLITHQSLKNNPSLAKLIGYCCEDGVKGFVYDLNPLGS 121
           +WDQK E D    N+ +  +EVKL TH SL  +PSLAKLIGYCCED VKG VYDL+PLGS
Sbjct: 74  IWDQKPEFDNATTNEFLVREEVKLSTHPSLNGHPSLAKLIGYCCEDEVKGVVYDLDPLGS 133

Query: 122 LQNLRGRDDHINWLQRVDVILELARLLDFIHNQENQSLGFHFRASNILLDWECKPKLCGF 181
           LQNL  RDD +NWLQRV+VILELARLLDFIH+QE QSL   FRASNILLDWECKPKLCG 
Sbjct: 134 LQNLVARDD-LNWLQRVEVILELARLLDFIHSQEKQSLPIKFRASNILLDWECKPKLCG- 193

Query: 182 KLISDNGLDADLYKLSKFTKLPFGYFHPSIRGESEGKVSSDVYSLGEILLGLIAKRDVEP 241
                 G D     L+ F + P  YFH S RG S+GK+S+D+YSLGEILLGLIAKR  EP
Sbjct: 194 -----TGAD-----LNNFAQNPSSYFHLSTRGGSQGKMSTDIYSLGEILLGLIAKRAYEP 253

Query: 242 QSLGKQNRQEVVNSSVSIWAKNEYRPNVSLVHESLQKDWGYSTEEGIKLTELGMHSIEFF 301
           + L ++N   VVNS VS WAKNEYRPN SLVHE+LQKDWGY+ EEG++LTEL +HSIEFF
Sbjct: 254 EKLERENHHLVVNSLVSNWAKNEYRPNGSLVHETLQKDWGYTAEEGVELTELAIHSIEFF 313

Query: 302 PRNRPSIKQILQHLEALQVTQRLSDVRPRKKERKLPSSSMGM 343
           PRNRPSIKQILQHLE LQVTQRLSD RPRKKERK PSS+MG+
Sbjct: 314 PRNRPSIKQILQHLEGLQVTQRLSDTRPRKKERKSPSSAMGI 341

[Close]

BLAST of IVF0025015 vs. NCBI nr
Match: XP_008446023.1 (PREDICTED: protein kinase APK1B, chloroplastic-like [Cucumis melo] >KAA0034186.1 protein kinase APK1B [Cucumis melo var. makuwa] >TYK15734.1 protein kinase APK1B [Cucumis melo var. makuwa])

HSP 1 Score: 691 bits (1784), Expect = 7.16e-251
Identity = 342/342 (100.00%), Postives = 342/342 (100.00%), Query Frame = 0


Query: 1   MADSENGNVLIFTFEDLKSFTNNFNGNNLIGITQSGRFYRGQLRPPWLAATITETRIVTV 60
           MADSENGNVLIFTFEDLKSFTNNFNGNNLIGITQSGRFYRGQLRPPWLAATITETRIVTV
Sbjct: 15  MADSENGNVLIFTFEDLKSFTNNFNGNNLIGITQSGRFYRGQLRPPWLAATITETRIVTV 74

Query: 61  KLWDQKQELDKEKVNQVYFQEVKLITHQSLKNNPSLAKLIGYCCEDGVKGFVYDLNPLGS 120
           KLWDQKQELDKEKVNQVYFQEVKLITHQSLKNNPSLAKLIGYCCEDGVKGFVYDLNPLGS
Sbjct: 75  KLWDQKQELDKEKVNQVYFQEVKLITHQSLKNNPSLAKLIGYCCEDGVKGFVYDLNPLGS 134

Query: 121 LQNLRGRDDHINWLQRVDVILELARLLDFIHNQENQSLGFHFRASNILLDWECKPKLCGF 180
           LQNLRGRDDHINWLQRVDVILELARLLDFIHNQENQSLGFHFRASNILLDWECKPKLCGF
Sbjct: 135 LQNLRGRDDHINWLQRVDVILELARLLDFIHNQENQSLGFHFRASNILLDWECKPKLCGF 194

Query: 181 KLISDNGLDADLYKLSKFTKLPFGYFHPSIRGESEGKVSSDVYSLGEILLGLIAKRDVEP 240
           KLISDNGLDADLYKLSKFTKLPFGYFHPSIRGESEGKVSSDVYSLGEILLGLIAKRDVEP
Sbjct: 195 KLISDNGLDADLYKLSKFTKLPFGYFHPSIRGESEGKVSSDVYSLGEILLGLIAKRDVEP 254

Query: 241 QSLGKQNRQEVVNSSVSIWAKNEYRPNVSLVHESLQKDWGYSTEEGIKLTELGMHSIEFF 300
           QSLGKQNRQEVVNSSVSIWAKNEYRPNVSLVHESLQKDWGYSTEEGIKLTELGMHSIEFF
Sbjct: 255 QSLGKQNRQEVVNSSVSIWAKNEYRPNVSLVHESLQKDWGYSTEEGIKLTELGMHSIEFF 314

Query: 301 PRNRPSIKQILQHLEALQVTQRLSDVRPRKKERKLPSSSMGM 342
           PRNRPSIKQILQHLEALQVTQRLSDVRPRKKERKLPSSSMGM
Sbjct: 315 PRNRPSIKQILQHLEALQVTQRLSDVRPRKKERKLPSSSMGM 356

[Close]

BLAST of IVF0025015 vs. NCBI nr
Match: XP_031741383.1 (serine/threonine-protein kinase BIK1 isoform X2 [Cucumis sativus])

HSP 1 Score: 624 bits (1609), Expect = 2.07e-224
Identity = 310/343 (90.38%), Postives = 325/343 (94.75%), Query Frame = 0


Query: 1   MADSENGNVLIFTFEDLKSFTNNFNGNNLIGITQSGRFYRGQLRPPWLAATITETRIVTV 60
           MAD ENGNVLIFTFEDLKSFTNNFNGNNLIGITQSGRFYRGQLRPPWLAA ITETRIVTV
Sbjct: 1   MADFENGNVLIFTFEDLKSFTNNFNGNNLIGITQSGRFYRGQLRPPWLAAIITETRIVTV 60

Query: 61  KLWDQKQELDKEKVNQVYFQE-VKLITHQSLKNNPSLAKLIGYCCEDGVKGFVYDLNPLG 120
           K+WDQKQEL+KEKVNQ+YFQE +KLITHQSL+NNPSLAKLIGYCCEDGVKGFVYDLNPLG
Sbjct: 61  KIWDQKQELEKEKVNQLYFQEELKLITHQSLRNNPSLAKLIGYCCEDGVKGFVYDLNPLG 120

Query: 121 SLQNLRGRDDHINWLQRVDVILELARLLDFIHNQENQSLGFHFRASNILLDWECKPKLCG 180
            LQNL  RDD INW QRVDVILELARLLDFIH+QENQSLGFHF ASNILLDW+CKPKLC 
Sbjct: 121 LLQNLMARDDRINWFQRVDVILELARLLDFIHSQENQSLGFHFSASNILLDWDCKPKLCR 180

Query: 181 FKLISDNGLDADLYKLSKFTKLPFGYFHPSIRGESEGKVSSDVYSLGEILLGLIAKRDVE 240
           FK ISD+ ++ADLYKLSKFT+LP GYFHPSIRGESEGKV+SDVYSLGEILLGLIAKRDVE
Sbjct: 181 FKPISDDRMNADLYKLSKFTQLPSGYFHPSIRGESEGKVNSDVYSLGEILLGLIAKRDVE 240

Query: 241 PQSLGKQNRQEVVNSSVSIWAKNEYRPNVSLVHESLQKDWGYSTEEGIKLTELGMHSIEF 300
           PQ+L KQN QE VNSSVSIWAKNEYRPNVSLVHESLQKDWGYSTEEGIKLTEL MHSIEF
Sbjct: 241 PQNLEKQNHQEFVNSSVSIWAKNEYRPNVSLVHESLQKDWGYSTEEGIKLTELAMHSIEF 300

Query: 301 FPRNRPSIKQILQHLEALQVTQRLSDVRPRKKERKLPSSSMGM 342
           FPRNRPSIKQILQHLEALQVT+RLSD RPRKKERKLPSSS+GM
Sbjct: 301 FPRNRPSIKQILQHLEALQVTRRLSDDRPRKKERKLPSSSVGM 343

[Close]

BLAST of IVF0025015 vs. NCBI nr
Match: XP_031741382.1 (serine/threonine-protein kinase BIK1 isoform X1 [Cucumis sativus] >KAE8648675.1 hypothetical protein Csa_008749 [Cucumis sativus])

HSP 1 Score: 610 bits (1572), Expect = 2.35e-218
Identity = 310/369 (84.01%), Postives = 325/369 (88.08%), Query Frame = 0


Query: 1   MADSENGNVLIFTFEDLKSFTNNFNGNNLIGITQSGRFYRGQLRPPWLAATITETRIVTV 60
           MAD ENGNVLIFTFEDLKSFTNNFNGNNLIGITQSGRFYRGQLRPPWLAA ITETRIVTV
Sbjct: 1   MADFENGNVLIFTFEDLKSFTNNFNGNNLIGITQSGRFYRGQLRPPWLAAIITETRIVTV 60

Query: 61  KLWDQKQELDKEKVNQVYFQE-VKLITHQSLKNNPSLAKLIGYCCEDGVKGFVYDLNPLG 120
           K+WDQKQEL+KEKVNQ+YFQE +KLITHQSL+NNPSLAKLIGYCCEDGVKGFVYDLNPLG
Sbjct: 61  KIWDQKQELEKEKVNQLYFQEELKLITHQSLRNNPSLAKLIGYCCEDGVKGFVYDLNPLG 120

Query: 121 SLQNLRGRDDHINWLQRVDVILELARLLDFIHNQENQSLGFHFRASNILLDW-------- 180
            LQNL  RDD INW QRVDVILELARLLDFIH+QENQSLGFHF ASNILLDW        
Sbjct: 121 LLQNLMARDDRINWFQRVDVILELARLLDFIHSQENQSLGFHFSASNILLDWVSCLLTLN 180

Query: 181 ------------------ECKPKLCGFKLISDNGLDADLYKLSKFTKLPFGYFHPSIRGE 240
                             +CKPKLC FK ISD+ ++ADLYKLSKFT+LP GYFHPSIRGE
Sbjct: 181 NEELSHESSTLLICSFQQDCKPKLCRFKPISDDRMNADLYKLSKFTQLPSGYFHPSIRGE 240

Query: 241 SEGKVSSDVYSLGEILLGLIAKRDVEPQSLGKQNRQEVVNSSVSIWAKNEYRPNVSLVHE 300
           SEGKV+SDVYSLGEILLGLIAKRDVEPQ+L KQN QE VNSSVSIWAKNEYRPNVSLVHE
Sbjct: 241 SEGKVNSDVYSLGEILLGLIAKRDVEPQNLEKQNHQEFVNSSVSIWAKNEYRPNVSLVHE 300

Query: 301 SLQKDWGYSTEEGIKLTELGMHSIEFFPRNRPSIKQILQHLEALQVTQRLSDVRPRKKER 342
           SLQKDWGYSTEEGIKLTEL MHSIEFFPRNRPSIKQILQHLEALQVT+RLSD RPRKKER
Sbjct: 301 SLQKDWGYSTEEGIKLTELAMHSIEFFPRNRPSIKQILQHLEALQVTRRLSDDRPRKKER 360

[Close]

BLAST of IVF0025015 vs. NCBI nr
Match: XP_022966807.1 (probable serine/threonine-protein kinase CST [Cucurbita maxima])

HSP 1 Score: 466 bits (1199), Expect = 6.58e-162
Identity = 243/342 (71.05%), Postives = 276/342 (80.70%), Query Frame = 0


Query: 2   ADSENGNVLIFTFEDLKSFTNNFNGNNLIGITQSGRFYRGQLRPPWLAATITETRIVTVK 61
           ADS NGNVLIFT EDLK+ T+NF+GN+LIGITQS RFYRGQLR  W  +T TE R +TVK
Sbjct: 14  ADSANGNVLIFTSEDLKTLTSNFHGNSLIGITQSCRFYRGQLRAAW--STGTEARNLTVK 73

Query: 62  LWDQKQELDKEKVNQVYF-QEVKLITHQSLKNNPSLAKLIGYCCEDGVKGFVYDLNPLGS 121
           +WDQK E D    N+    +EVKL TH SL  + SLAKLIGYCCED VKG VYD +PLGS
Sbjct: 74  IWDQKPEFDNAITNEFLVREEVKLSTHSSLNGHRSLAKLIGYCCEDEVKGVVYDFDPLGS 133

Query: 122 LQNLRGRDDHINWLQRVDVILELARLLDFIHNQENQSLGFHFRASNILLDWECKPKLCGF 181
           LQNL  RDD +NWLQRV+VILELARLLDFIH+QE QSL   FRASNILLDWECKPKLCG 
Sbjct: 134 LQNLVARDD-LNWLQRVEVILELARLLDFIHSQEKQSLPIKFRASNILLDWECKPKLCGL 193

Query: 182 KLISDNGLDADLYKLSKFTKLPFGYFHPSIRGESEGKVSSDVYSLGEILLGLIAKRDVEP 241
           ++IS NG  ADL   + F + P  YFH S RG S+GK+S+D++SLGEILLGLIAKRD EP
Sbjct: 194 EVIS-NGAGADL---NNFAQNPSSYFHLSTRGGSQGKMSTDIFSLGEILLGLIAKRDYEP 253

Query: 242 QSLGKQNRQEVVNSSVSIWAKNEYRPNVSLVHESLQKDWGYSTEEGIKLTELGMHSIEFF 301
           + L ++N   VVNS VS WAKNEYRPN SLVHE+LQKDWGY+ EEG++LTEL +HSIEFF
Sbjct: 254 EKLERENHHRVVNSLVSNWAKNEYRPNGSLVHETLQKDWGYTVEEGVELTELAIHSIEFF 313

Query: 302 PRNRPSIKQILQHLEALQVTQRLSDVRPRKKERKLPSSSMGM 342
           PRNRPSIKQILQ LE LQVTQRLSD RPRKKERK PSS+MG+
Sbjct: 314 PRNRPSIKQILQRLEGLQVTQRLSDTRPRKKERKSPSSAMGI 348

[Close]

BLAST of IVF0025015 vs. NCBI nr
Match: XP_022945250.1 (serine/threonine-protein kinase BIK1-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 462 bits (1190), Expect = 1.20e-160
Identity = 242/342 (70.76%), Postives = 272/342 (79.53%), Query Frame = 0


Query: 2   ADSENGNVLIFTFEDLKSFTNNFNGNNLIGITQSGRFYRGQLRPPWLAATITETRIVTVK 61
           ADS NGNVLIFT EDLK+ T+NF+ N+LIGITQSGRFYRGQLR  W  +T TE R +TVK
Sbjct: 14  ADSANGNVLIFTSEDLKTLTSNFHSNSLIGITQSGRFYRGQLRSAW--STGTEARNLTVK 73

Query: 62  LWDQKQELDKEKVNQVYF-QEVKLITHQSLKNNPSLAKLIGYCCEDGVKGFVYDLNPLGS 121
           +WDQK E D    N+    +EVKL TH SL  +PSLAKLIGYCCED VKG VYDL+PLGS
Sbjct: 74  IWDQKPEFDNATTNEFLVREEVKLSTHPSLNGHPSLAKLIGYCCEDEVKGVVYDLDPLGS 133

Query: 122 LQNLRGRDDHINWLQRVDVILELARLLDFIHNQENQSLGFHFRASNILLDWECKPKLCGF 181
           LQNL  RDD +NWLQRV+VILELARLLDFIH+QE QSL   FRASNILLDWECKPKLCG 
Sbjct: 134 LQNLVARDD-LNWLQRVEVILELARLLDFIHSQEKQSLPIKFRASNILLDWECKPKLCG- 193

Query: 182 KLISDNGLDADLYKLSKFTKLPFGYFHPSIRGESEGKVSSDVYSLGEILLGLIAKRDVEP 241
                 G D     L+ F + P  YFH S RG S+GK+S+D+YSLGEILLGLIAKR  EP
Sbjct: 194 -----TGAD-----LNNFAQNPSSYFHLSTRGGSQGKMSTDIYSLGEILLGLIAKRAYEP 253

Query: 242 QSLGKQNRQEVVNSSVSIWAKNEYRPNVSLVHESLQKDWGYSTEEGIKLTELGMHSIEFF 301
           + L ++N   VVNS VS WAKNEYRPN SLVHE+LQKDWGY+ EEG++LTEL +HSIEFF
Sbjct: 254 EKLERENHHLVVNSLVSNWAKNEYRPNGSLVHETLQKDWGYTAEEGVELTELAIHSIEFF 313

Query: 302 PRNRPSIKQILQHLEALQVTQRLSDVRPRKKERKLPSSSMGM 342
           PRNRPSIKQILQHLE LQVTQRLSD RPRKKERK PSS+MG+
Sbjct: 314 PRNRPSIKQILQHLEGLQVTQRLSDTRPRKKERKSPSSAMGI 341

[Close]

BLAST of IVF0025015 vs. TAIR 10
Match: AT2G28930.1 (protein kinase 1B )

HSP 1 Score: 123.6 bits (309), Expect = 3.0e-28
Identity = 103/341 (30.21%), Postives = 162/341 (47.51%), Query Frame = 0


Query: 5   ENGNVLIFTFEDLKSFTNNFNGNNLIGITQSGRFYRGQLRPPWLAATITETRIVTVKLWD 64
           ++ N+  FTF +LK+ T NF  ++++G    G  ++G +    L A+   T +V      
Sbjct: 61  QSPNLKSFTFAELKAATRNFRPDSVLGEGGFGSVFKGWIDEQTLTASKPGTGVVIAV--- 120

Query: 65  QKQELDKEKVNQVYFQEVKLITHQSLKNNPSLAKLIGYCCEDGVKGFVYDLNPLGSLQN- 124
           +K   D  + +Q +  EV  +   S   +P+L KLIGYC ED  +  VY+  P GSL+N 
Sbjct: 121 KKLNQDGWQGHQEWLAEVNYLGQFS---HPNLVKLIGYCLEDEHRLLVYEFMPRGSLENH 180

Query: 125 LRGRDDH---INWLQRVDVILELARLLDFIHNQENQSLGFHFRASNILLDWECKPKLCGF 184
           L  R  +   ++W  R+ V L  A+ L F+HN E   +   F+ SNILLD E   KL  F
Sbjct: 181 LFRRGSYFQPLSWTLRLKVALGAAKGLAFLHNAETSVIYRDFKTSNILLDSEYNAKLSDF 240

Query: 185 KLISDNGLDADLYKLSKFTKLPFGYFHPSIRGESEGKVSSDVYSLGEILLGLIAKRDVEP 244
            L  D G   D   +S      +GY  P           SDVYS G +LL +++ R    
Sbjct: 241 GLAKD-GPTGDKSHVSTRIMGTYGYAAPEYLATGHLTTKSDVYSYGVVLLEVLSGRRAVD 300

Query: 245 QSLGKQNRQEVVNSSVSIWAKNEYRPNVSLVHESLQKDWGYSTEEGIKLTELGMHSIEFF 304
           ++      Q++V  +  + A    R    ++   LQ    YS EE  K+  L +  + F 
Sbjct: 301 KN-RPPGEQKLVEWARPLLANK--RKLFRVIDNRLQDQ--YSMEEACKVATLALRCLTFE 360

Query: 305 PRNRPSIKQILQHLEALQVTQ----RLSDVRPRKKERKLPS 338
            + RP++ +++ HLE +Q       R  D+  R+  R+  S
Sbjct: 361 IKLRPNMNEVVSHLEHIQTLNEAGGRNIDMVQRRMRRRSDS 389

[Close]

BLAST of IVF0025015 vs. TAIR 10
Match: AT2G28930.2 (protein kinase 1B )

HSP 1 Score: 123.6 bits (309), Expect = 3.0e-28
Identity = 103/341 (30.21%), Postives = 162/341 (47.51%), Query Frame = 0


Query: 5   ENGNVLIFTFEDLKSFTNNFNGNNLIGITQSGRFYRGQLRPPWLAATITETRIVTVKLWD 64
           ++ N+  FTF +LK+ T NF  ++++G    G  ++G +    L A+   T +V      
Sbjct: 50  QSPNLKSFTFAELKAATRNFRPDSVLGEGGFGSVFKGWIDEQTLTASKPGTGVVIAV--- 109

Query: 65  QKQELDKEKVNQVYFQEVKLITHQSLKNNPSLAKLIGYCCEDGVKGFVYDLNPLGSLQN- 124
           +K   D  + +Q +  EV  +   S   +P+L KLIGYC ED  +  VY+  P GSL+N 
Sbjct: 110 KKLNQDGWQGHQEWLAEVNYLGQFS---HPNLVKLIGYCLEDEHRLLVYEFMPRGSLENH 169

Query: 125 LRGRDDH---INWLQRVDVILELARLLDFIHNQENQSLGFHFRASNILLDWECKPKLCGF 184
           L  R  +   ++W  R+ V L  A+ L F+HN E   +   F+ SNILLD E   KL  F
Sbjct: 170 LFRRGSYFQPLSWTLRLKVALGAAKGLAFLHNAETSVIYRDFKTSNILLDSEYNAKLSDF 229

Query: 185 KLISDNGLDADLYKLSKFTKLPFGYFHPSIRGESEGKVSSDVYSLGEILLGLIAKRDVEP 244
            L  D G   D   +S      +GY  P           SDVYS G +LL +++ R    
Sbjct: 230 GLAKD-GPTGDKSHVSTRIMGTYGYAAPEYLATGHLTTKSDVYSYGVVLLEVLSGRRAVD 289

Query: 245 QSLGKQNRQEVVNSSVSIWAKNEYRPNVSLVHESLQKDWGYSTEEGIKLTELGMHSIEFF 304
           ++      Q++V  +  + A    R    ++   LQ    YS EE  K+  L +  + F 
Sbjct: 290 KN-RPPGEQKLVEWARPLLANK--RKLFRVIDNRLQDQ--YSMEEACKVATLALRCLTFE 349

Query: 305 PRNRPSIKQILQHLEALQVTQ----RLSDVRPRKKERKLPS 338
            + RP++ +++ HLE +Q       R  D+  R+  R+  S
Sbjct: 350 IKLRPNMNEVVSHLEHIQTLNEAGGRNIDMVQRRMRRRSDS 378

[Close]

BLAST of IVF0025015 vs. TAIR 10
Match: AT2G28930.3 (protein kinase 1B )

HSP 1 Score: 123.6 bits (309), Expect = 3.0e-28
Identity = 103/341 (30.21%), Postives = 162/341 (47.51%), Query Frame = 0


Query: 5   ENGNVLIFTFEDLKSFTNNFNGNNLIGITQSGRFYRGQLRPPWLAATITETRIVTVKLWD 64
           ++ N+  FTF +LK+ T NF  ++++G    G  ++G +    L A+   T +V      
Sbjct: 53  QSPNLKSFTFAELKAATRNFRPDSVLGEGGFGSVFKGWIDEQTLTASKPGTGVVIAV--- 112

Query: 65  QKQELDKEKVNQVYFQEVKLITHQSLKNNPSLAKLIGYCCEDGVKGFVYDLNPLGSLQN- 124
           +K   D  + +Q +  EV  +   S   +P+L KLIGYC ED  +  VY+  P GSL+N 
Sbjct: 113 KKLNQDGWQGHQEWLAEVNYLGQFS---HPNLVKLIGYCLEDEHRLLVYEFMPRGSLENH 172

Query: 125 LRGRDDH---INWLQRVDVILELARLLDFIHNQENQSLGFHFRASNILLDWECKPKLCGF 184
           L  R  +   ++W  R+ V L  A+ L F+HN E   +   F+ SNILLD E   KL  F
Sbjct: 173 LFRRGSYFQPLSWTLRLKVALGAAKGLAFLHNAETSVIYRDFKTSNILLDSEYNAKLSDF 232

Query: 185 KLISDNGLDADLYKLSKFTKLPFGYFHPSIRGESEGKVSSDVYSLGEILLGLIAKRDVEP 244
            L  D G   D   +S      +GY  P           SDVYS G +LL +++ R    
Sbjct: 233 GLAKD-GPTGDKSHVSTRIMGTYGYAAPEYLATGHLTTKSDVYSYGVVLLEVLSGRRAVD 292

Query: 245 QSLGKQNRQEVVNSSVSIWAKNEYRPNVSLVHESLQKDWGYSTEEGIKLTELGMHSIEFF 304
           ++      Q++V  +  + A    R    ++   LQ    YS EE  K+  L +  + F 
Sbjct: 293 KN-RPPGEQKLVEWARPLLANK--RKLFRVIDNRLQDQ--YSMEEACKVATLALRCLTFE 352

Query: 305 PRNRPSIKQILQHLEALQVTQ----RLSDVRPRKKERKLPS 338
            + RP++ +++ HLE +Q       R  D+  R+  R+  S
Sbjct: 353 IKLRPNMNEVVSHLEHIQTLNEAGGRNIDMVQRRMRRRSDS 381

[Close]

BLAST of IVF0025015 vs. TAIR 10
Match: AT2G39660.1 (botrytis-induced kinase1 )

HSP 1 Score: 118.2 bits (295), Expect = 1.3e-26
Identity = 101/337 (29.97%), Postives = 159/337 (47.18%), Query Frame = 0


Query: 12  FTFEDLKSFTNNFNGNNLIGITQSGRFYRGQLRPPWLAATITETRIVTVKLWDQKQELDK 71
           FTF +LK  T NF  +++IG    G  ++G L    L  T   T +V          +  
Sbjct: 55  FTFNELKLATRNFRPDSVIGEGGFGCVFKGWLDESTLTPTKPGTGLV----------IAV 114

Query: 72  EKVNQVYFQE----VKLITHQSLKNNPSLAKLIGYCCEDGVKGFVYDLNPLGSLQNLRGR 131
           +K+NQ  FQ     +  I +    ++P+L KLIGYC ED  +  VY+    GSL+N   R
Sbjct: 115 KKLNQEGFQGHREWLTEINYLGQLSHPNLVKLIGYCLEDEHRLLVYEFMQKGSLENHLFR 174

Query: 132 D----DHINWLQRVDVILELARLLDFIHNQENQSLGFHFRASNILLDWECKPKLCGFKLI 191
                  + W  RV+V L+ A+ L F+H+   + +    +ASNILLD +   KL  F L 
Sbjct: 175 RGAYFKPLPWFLRVNVALDAAKGLAFLHSDPVKVIYRDIKASNILLDADYNAKLSDFGLA 234

Query: 192 SDNGLDADLYKLSKFTKLPFGYFHPSIRGESEGKVSSDVYSLGEILLGLIA-KRDVEPQS 251
            D G   DL  +S      +GY  P           SDVYS G +LL +++ KR ++   
Sbjct: 235 RD-GPMGDLSYVSTRVMGTYGYAAPEYMSSGHLNARSDVYSFGVLLLEILSGKRALDHNR 294

Query: 252 LGKQNRQEVVNSSVSIWAK---NEYRPNVSLVHESLQKDWGYSTEEGIKLTELGMHSIEF 311
             K+        ++  WA+      R  + +V   L  D  Y  EE +++  + +  + F
Sbjct: 295 PAKE-------ENLVDWARPYLTSKRKVLLIVDNRL--DTQYLPEEAVRMASVAVQCLSF 354

Query: 312 FPRNRPSIKQILQHLEALQVT-QRLSDVRPRKKERKL 336
            P++RP++ Q+++ L+ LQ    + S   P K  +KL
Sbjct: 355 EPKSRPTMDQVVRALQQLQDNLGKPSQTNPVKDTKKL 371

[Close]

BLAST of IVF0025015 vs. TAIR 10
Match: AT4G35600.1 (Protein kinase superfamily protein )

HSP 1 Score: 116.7 bits (291), Expect = 3.7e-26
Identity = 96/336 (28.57%), Postives = 156/336 (46.43%), Query Frame = 0


Query: 5   ENGNVLIFTFEDLKSFTNNFNGNNLIGITQSGRFYRGQLRPPWLAATITETRIVTVKLWD 64
           E+ N+ ++ F DLK+ T NF  ++++G    G+ YRG     W+ AT      V   +  
Sbjct: 67  ESPNLKVYNFLDLKTATKNFKPDSMLGQGGFGKVYRG-----WVDATTLAPSRVGSGMIV 126

Query: 65  QKQELDKEKVNQVYFQEVKLITHQSLKNNPSLAKLIGYCCEDGVKGFVYDLNPLGSLQ-N 124
             + L+ E V Q + +    +    + ++ +L KL+GYC ED     VY+  P GSL+ +
Sbjct: 127 AIKRLNSESV-QGFAEWRSEVNFLGMLSHRNLVKLLGYCREDKELLLVYEFMPKGSLESH 186

Query: 125 LRGRDDHINWLQRVDVILELARLLDFIHNQENQSLGFHFRASNILLDWECKPKLCGFKLI 184
           L  R+D   W  R+ +++  AR L F+H+ + + +   F+ASNILLD     KL  F L 
Sbjct: 187 LFRRNDPFPWDLRIKIVIGAARGLAFLHSLQREVIYRDFKASNILLDSNYDAKLSDFGLA 246

Query: 185 SDNGLDADLYKLSKFTKLPFGYFHPSIRGESEGKVSSDVYSLG----EILLGLIAKRDVE 244
                D   +  ++     +GY  P         V SDV++ G    EI+ GL A     
Sbjct: 247 KLGPADEKSHVTTRIMG-TYGYAAPEYMATGHLYVKSDVFAFGVVLLEIMTGLTAHNTKR 306

Query: 245 PQSLGKQNRQEVVNSSVSIWAKNEYRPNVSLVHESLQ-KDWG----YSTEEGIKLTELGM 304
           P+             S+  W     RP +S  H   Q  D G    Y+T+   ++  + +
Sbjct: 307 PRG----------QESLVDW----LRPELSNKHRVKQIMDKGIKGQYTTKVATEMARITL 366

Query: 305 HSIEFFPRNRPSIK---QILQHLEALQVTQRLSDVR 328
             IE  P+NRP +K   ++L+H++ L V    S  +
Sbjct: 367 SCIEPDPKNRPHMKEVVEVLEHIQGLNVVPNRSSTK 381

The following BLAST results are available for this feature:

BLAST of IVF0025015 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P46573	4.2e-27	30.21	Probable serine/threonine-protein kinase PBL10 OS=Arabidopsis thaliana OX=3702 G...	[more]
O48814	1.8e-25	29.97	Serine/threonine-protein kinase BIK1 OS=Arabidopsis thaliana OX=3702 GN=BIK1 PE=...	[more]
P27450	5.2e-25	28.57	Probable serine/threonine-protein kinase CST OS=Arabidopsis thaliana OX=3702 GN=...	[more]
Q8H186	1.5e-24	28.57	Probable serine/threonine-protein kinase PBL1 OS=Arabidopsis thaliana OX=3702 GN...	[more]
Q06548	1.5e-24	30.22	Probable serine/threonine-protein kinase PBL9 OS=Arabidopsis thaliana OX=3702 GN...	[more]

BLAST of IVF0025015 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7SW68	7.6e-197	100.00	Protein kinase APK1B OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G...	[more]
A0A1S3BEW9	7.6e-197	100.00	protein kinase APK1B, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103488875...	[more]
A0A0A0KPY2	1.5e-176	90.38	Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G589...	[more]
A0A6J1HNY6	6.8e-129	71.05	probable serine/threonine-protein kinase CST OS=Cucurbita maxima OX=3661 GN=LOC1...	[more]
A0A6J1G0E5	7.5e-128	70.76	serine/threonine-protein kinase BIK1-like isoform X2 OS=Cucurbita moschata OX=36...	[more]

BLAST of IVF0025015 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008446023.1	7.16e-251	100.00	PREDICTED: protein kinase APK1B, chloroplastic-like [Cucumis melo] >KAA0034186.1...	[more]
XP_031741383.1	2.07e-224	90.38	serine/threonine-protein kinase BIK1 isoform X2 [Cucumis sativus]	[more]
XP_031741382.1	2.35e-218	84.01	serine/threonine-protein kinase BIK1 isoform X1 [Cucumis sativus] >KAE8648675.1 ...	[more]
XP_022966807.1	6.58e-162	71.05	probable serine/threonine-protein kinase CST [Cucurbita maxima]	[more]
XP_022945250.1	1.20e-160	70.76	serine/threonine-protein kinase BIK1-like isoform X2 [Cucurbita moschata]	[more]

BLAST of IVF0025015 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G28930.1	3.0e-28	30.21	protein kinase 1B	[more]
AT2G28930.2	3.0e-28	30.21	protein kinase 1B	[more]
AT2G28930.3	3.0e-28	30.21	protein kinase 1B	[more]
AT2G39660.1	1.3e-26	29.97	botrytis-induced kinase1	[more]
AT4G35600.1	3.7e-26	28.57	Protein kinase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	1.10.510.10	Transferase(Phosphotransferase) domain 1	coord: 118..336 e-value: 3.4E-20 score: 74.1
None	No IPR available	GENE3D	3.30.200.20	Phosphorylase Kinase; domain 1	coord: 2..115 e-value: 3.0E-13 score: 51.7
None	No IPR available	PANTHER	PTHR45621:SF85	RECEPTOR-LIKE KINASE	coord: 7..323
None	No IPR available	PANTHER	PTHR45621	OS01G0588500 PROTEIN-RELATED	coord: 7..323
IPR001245	Serine-threonine/tyrosine-protein kinase, catalytic domain	PFAM	PF07714	PK_Tyr_Ser-Thr	coord: 29..252 e-value: 7.6E-13 score: 48.3
IPR000719	Protein kinase domain	PROSITE	PS50011	PROTEIN_KINASE_DOM	coord: 24..316 score: 17.149502
IPR011009	Protein kinase-like domain superfamily	SUPERFAMILY	56112	Protein kinase-like (PK-like)	coord: 8..317

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
IVF0025015.2	IVF0025015.2	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006468	protein phosphorylation
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004672	protein kinase activity