IVF0024251 (gene) Melon (IVF77) v1

Overview
NameIVF0024251
Typegene
OrganismCucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
Description(1->3)-beta-glucan endohydrolase
Locationchr04: 2827606 .. 2829573 (+)
RNA-Seq ExpressionIVF0024251
SyntenyIVF0024251
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAATCGTTGAAATTTGTGGTTTCTTGGAGATTTTTTCTTGTTCTGTTGCTTTCTTCAGCAGCGGTGAGTTCAGTGATTGGTATCGGAGTGAACTGGGGAACTCAGGCTACTCATCCGTTGCCGCCAGGGACGGTGGTGAAGATGCTGAAGGATAACGGGATTCAGAAGGTTAAGCTTTTTGATGCCGATCCTGTAGTTCTAAAGGCTCTTGGCAAATCGGGGATTGAAGTGATGGTGGGAATTCCAAACGATTTGCTCTACAGCTTGGCTAATAGCATCCAATCAGCTGAAACTGGGTTGCTAAAAACTTGTCTTCTTATATTTCATCTGGCAGTGTGGATATAAGGTAAAAGACAAACAATCAACAATCTCTCTCTCATTTATCTCGTAACTTTATGTGATGGCTGAATAACTTTGTCTTTTTTTGGCTGTTTCTTCTGATTTTGCTGCTAGAAACTACACAACTGGCTTGGGTTTTGTTCCCCGGAATGATTCTGTTTCTGTTGGAATATGTGTATTCTTATTATTTACAATTTTAGCTCTAAGGTTTAGAGAAATACGCTATATAAACTCGACTTTAGCTCCAGAATTCAGAGAATCATGCTATACAAACTCTTTGACCTAGAGGCGCATTCGACATGTGTTTTGAAATATTTCCTTCTATAGTAAATTGTTTTCCTTCTGCCATAGATGTGACTAATACACTCTTAGTAAATCATGATAGTAAATCATTTAAACTTGTGTTGATTCTCTATTGATTTATGTTTTTTGTTTCATCTTTATTTGTCGATTTCTCAATGGTATTATTGGGATGGAGAATTTTGACTTGATTTTGTATGTTTTACAAGGTATGTTGCAGTTGGGAATGAACCGTTTTTGAAAACATTCAACGGAAGCTTTATAGGAACTACTTTTCCTGCTCTCCAAAATGTCCAAGCCGCCATTGTTAAGGCTGGTTTAAGCAATCGGGTAAAAGTAACAGTGCCTATGAATGCTGATGTTTATCAAACTTCCACAGGTCTCCCTTCAGGCGGCGACTTTCGTTCTGATATTCGTGACCTTATGCTCTCCATTGTCAAGTTCTTGAGCGACAGTGCATCCCCTTTTACTGTTAATATCTACCCTTTCATTAGCCTTTATGAAGATCCCAGTTTCCCAATTGACTATGCCTTCTTTGATGGTTTCTCGCATGCTTTGAACGATAATGGTCAAATCTACGAAAATGTTTTTACTGCCAACTACGATACCCTTGTTTCGTCTTTACAGAAAAATGGCTTCCCTAACATGTCCATAATCGTTGGAGAGATTGGATGGCCAACAGATGGAGATCGGAACGCGAATCTGCAGTTTGCCAAGAGATTCAGCCAAGGCTTCATTTCGCATTTTCTTTCGGGGCAAGGAACGCCAATGAGGCCTGGTCCTATCGACGTCTACTTGTTTAGTCTTATAGACGAAGATGCTAAGAGCATTCTGCCAGGCAATTTCGAAAGGCATTGGGGTCTATTTTACTATGACGGTCGACCTAAATACATGCTTCAAATGACAAACAGCACAAATGGTTTGGTACCTGCATCAAATGTCAAGTATCTTGCAAAAAAATGGTGTGTCCTTGCACCATCAGCTAGCATCGACGATCCACAAATCGCCCCAAGTGTTAGCTACGCTTGCGAAAACGGTGATTGCACGAGCCTTGGCCTCGGGACTTCCTGCGGGACTCTGGATGCTCGCCAAAACATCTCGTATGCATTCAACTCGTATTACCAGATAAAGAATCAGTTGGCAAGTGCTTGTAAGTTTCCACATCTTTCAGTTATCACAACTCAAGATCCATCAGTTGGAAGTTGCAAGTTTGTTGTGATGACTCAACCTTCTGAAGTTGCTGTCAATGAAAAATCAAAGGCTTTGAGGCATTCTGCTGAATCATTGTTGTTTTTTCTTTGTTTGTTCATTCTGGCAGTTATGTGA

mRNA sequence

ATGAAATCGTTGAAATTTGTGGTTTCTTGGAGATTTTTTCTTGTTCTGTTGCTTTCTTCAGCAGCGGTGAGTTCAGTGATTGGTATCGGAGTGAACTGGGGAACTCAGGCTACTCATCCGTTGCCGCCAGGGACGGTGGTGAAGATGCTGAAGGATAACGGGATTCAGAAGGTTAAGCTTTTTGATGCCGATCCTGTAGTTCTAAAGGCTCTTGGCAAATCGGGGATTGAAGTGATGGTGGGAATTCCAAACGATTTGCTCTACAGCTTGGCTAATAGCATCCAATCAGCTGAAACTGGGTTGCTAAAAACTTGTCTTCTTATATTTCATCTGGCAGTGTATGTTGCAGTTGGGAATGAACCGTTTTTGAAAACATTCAACGGAAGCTTTATAGGAACTACTTTTCCTGCTCTCCAAAATGTCCAAGCCGCCATTGTTAAGGCTGGTTTAAGCAATCGGGTAAAAGTAACAGTGCCTATGAATGCTGATGTTTATCAAACTTCCACAGGTCTCCCTTCAGGCGGCGACTTTCGTTCTGATATTCGTGACCTTATGCTCTCCATTGTCAAGTTCTTGAGCGACAGTGCATCCCCTTTTACTGTTAATATCTACCCTTTCATTAGCCTTTATGAAGATCCCAGTTTCCCAATTGACTATGCCTTCTTTGATGGTTTCTCGCATGCTTTGAACGATAATGGTCAAATCTACGAAAATGTTTTTACTGCCAACTACGATACCCTTGTTTCGTCTTTACAGAAAAATGGCTTCCCTAACATGTCCATAATCGTTGGAGAGATTGGATGGCCAACAGATGGAGATCGGAACGCGAATCTGCAGTTTGCCAAGAGATTCAGCCAAGGCTTCATTTCGCATTTTCTTTCGGGGCAAGGAACGCCAATGAGGCCTGGTCCTATCGACGTCTACTTGTTTAGTCTTATAGACGAAGATGCTAAGAGCATTCTGCCAGGCAATTTCGAAAGGCATTGGGGTCTATTTTACTATGACGGTCGACCTAAATACATGCTTCAAATGACAAACAGCACAAATGGTTTGGTACCTGCATCAAATGTCAAGTATCTTGCAAAAAAATGGTGTGTCCTTGCACCATCAGCTAGCATCGACGATCCACAAATCGCCCCAAGTGTTAGCTACGCTTGCGAAAACGGTGATTGCACGAGCCTTGGCCTCGGGACTTCCTGCGGGACTCTGGATGCTCGCCAAAACATCTCGTATGCATTCAACTCGTATTACCAGATAAAGAATCAGTTGGCAAGTGCTTGTAAGTTTCCACATCTTTCAGTTATCACAACTCAAGATCCATCAGTTGGAAGTTGCAAGTTTGTTGTGATGACTCAACCTTCTGAAGTTGCTGTCAATGAAAAATCAAAGGCTTTGAGGCATTCTGCTGAATCATTGTTGTTTTTTCTTTGTTTGTTCATTCTGGCAGTTATGTGA

Coding sequence (CDS)

ATGAAATCGTTGAAATTTGTGGTTTCTTGGAGATTTTTTCTTGTTCTGTTGCTTTCTTCAGCAGCGGTGAGTTCAGTGATTGGTATCGGAGTGAACTGGGGAACTCAGGCTACTCATCCGTTGCCGCCAGGGACGGTGGTGAAGATGCTGAAGGATAACGGGATTCAGAAGGTTAAGCTTTTTGATGCCGATCCTGTAGTTCTAAAGGCTCTTGGCAAATCGGGGATTGAAGTGATGGTGGGAATTCCAAACGATTTGCTCTACAGCTTGGCTAATAGCATCCAATCAGCTGAAACTGGGTTGCTAAAAACTTGTCTTCTTATATTTCATCTGGCAGTGTATGTTGCAGTTGGGAATGAACCGTTTTTGAAAACATTCAACGGAAGCTTTATAGGAACTACTTTTCCTGCTCTCCAAAATGTCCAAGCCGCCATTGTTAAGGCTGGTTTAAGCAATCGGGTAAAAGTAACAGTGCCTATGAATGCTGATGTTTATCAAACTTCCACAGGTCTCCCTTCAGGCGGCGACTTTCGTTCTGATATTCGTGACCTTATGCTCTCCATTGTCAAGTTCTTGAGCGACAGTGCATCCCCTTTTACTGTTAATATCTACCCTTTCATTAGCCTTTATGAAGATCCCAGTTTCCCAATTGACTATGCCTTCTTTGATGGTTTCTCGCATGCTTTGAACGATAATGGTCAAATCTACGAAAATGTTTTTACTGCCAACTACGATACCCTTGTTTCGTCTTTACAGAAAAATGGCTTCCCTAACATGTCCATAATCGTTGGAGAGATTGGATGGCCAACAGATGGAGATCGGAACGCGAATCTGCAGTTTGCCAAGAGATTCAGCCAAGGCTTCATTTCGCATTTTCTTTCGGGGCAAGGAACGCCAATGAGGCCTGGTCCTATCGACGTCTACTTGTTTAGTCTTATAGACGAAGATGCTAAGAGCATTCTGCCAGGCAATTTCGAAAGGCATTGGGGTCTATTTTACTATGACGGTCGACCTAAATACATGCTTCAAATGACAAACAGCACAAATGGTTTGGTACCTGCATCAAATGTCAAGTATCTTGCAAAAAAATGGTGTGTCCTTGCACCATCAGCTAGCATCGACGATCCACAAATCGCCCCAAGTGTTAGCTACGCTTGCGAAAACGGTGATTGCACGAGCCTTGGCCTCGGGACTTCCTGCGGGACTCTGGATGCTCGCCAAAACATCTCGTATGCATTCAACTCGTATTACCAGATAAAGAATCAGTTGGCAAGTGCTTGTAAGTTTCCACATCTTTCAGTTATCACAACTCAAGATCCATCAGTTGGAAGTTGCAAGTTTGTTGTGATGACTCAACCTTCTGAAGTTGCTGTCAATGAAAAATCAAAGGCTTTGAGGCATTCTGCTGAATCATTGTTGTTTTTTCTTTGTTTGTTCATTCTGGCAGTTATGTGA

Protein sequence

MKSLKFVVSWRFFLVLLLSSAAVSSVIGIGVNWGTQATHPLPPGTVVKMLKDNGIQKVKLFDADPVVLKALGKSGIEVMVGIPNDLLYSLANSIQSAETGLLKTCLLIFHLAVYVAVGNEPFLKTFNGSFIGTTFPALQNVQAAIVKAGLSNRVKVTVPMNADVYQTSTGLPSGGDFRSDIRDLMLSIVKFLSDSASPFTVNIYPFISLYEDPSFPIDYAFFDGFSHALNDNGQIYENVFTANYDTLVSSLQKNGFPNMSIIVGEIGWPTDGDRNANLQFAKRFSQGFISHFLSGQGTPMRPGPIDVYLFSLIDEDAKSILPGNFERHWGLFYYDGRPKYMLQMTNSTNGLVPASNVKYLAKKWCVLAPSASIDDPQIAPSVSYACENGDCTSLGLGTSCGTLDARQNISYAFNSYYQIKNQLASACKFPHLSVITTQDPSVGSCKFVVMTQPSEVAVNEKSKALRHSAESLLFFLCLFILAVM
Homology
BLAST of IVF0024251 vs. ExPASy Swiss-Prot
Match: Q9M088 (Glucan endo-1,3-beta-glucosidase 5 OS=Arabidopsis thaliana OX=3702 GN=At4g31140 PE=2 SV=1)

HSP 1 Score: 627.5 bits (1617), Expect = 1.3e-178
Identity = 298/477 (62.47%), Postives = 384/477 (80.50%), Query Frame = 0

Query: 12  FFLVLLLSSAAVSSVIGIGVNWGTQATHPLPPGTVVKMLKDNGIQKVKLFDADPVVLKAL 71
           FF++ LL ++ +  V GIGVNWG+QA HPLPP TVV++L++NGIQKVKLF+AD  +LKAL
Sbjct: 10  FFVITLLYASLLIEVEGIGVNWGSQARHPLPPATVVRLLRENGIQKVKLFEADSAILKAL 69

Query: 72  GKSGIEVMVGIPNDLLYSLANSIQSAETGLLKTCLLIFHLAV------YVAVGNEPFLKT 131
            ++GI+VMVGIPNDLL  LA S+ +AE  + +   +  H++       YVAVGNEPFLK 
Sbjct: 70  SRTGIQVMVGIPNDLLAPLAGSVAAAERWVSQN--VSAHVSSNGVDIRYVAVGNEPFLKA 129

Query: 132 FNGSFIGTTFPALQNVQAAIVKAGLSNRVKVTVPMNADVYQTSTGLPSGGDFRSDIRDLM 191
           FNG+F G T PALQN+Q+AI+KAGL+ +VKVTVP+NADVYQ+++ LPS GDFR +IRDLM
Sbjct: 130 FNGTFEGITLPALQNIQSAIIKAGLATQVKVTVPLNADVYQSASNLPSDGDFRPEIRDLM 189

Query: 192 LSIVKFLSDSASPFTVNIYPFISLYEDPSFPIDYAFFDGFSHALNDNGQIYENVFTANYD 251
           L+IVKFLSD+ +PFT+NIYPFISLY DP+FP+++AFFDG    +NDNG+IY+NV  ANYD
Sbjct: 190 LNIVKFLSDNGAPFTINIYPFISLYNDPNFPVEFAFFDGTGTPINDNGRIYDNVLDANYD 249

Query: 252 TLVSSLQKNGFPNMSIIVGEIGWPTDGDRNANLQFAKRFSQGFISHFLSGQGTPMRPGPI 311
           TLV SLQKNGF N++IIVGE+GWPTDGD+NANL +A+R++QGF++   + +GTPMRPG +
Sbjct: 250 TLVWSLQKNGFGNLTIIVGEVGWPTDGDKNANLMYARRYNQGFMNRQKANKGTPMRPGAM 309

Query: 312 DVYLFSLIDEDAKSILPGNFERHWGLFYYDGRPKYMLQMTNSTNGLVPASNVKYLAKKWC 371
           D YLF LIDEDAKSI PGNFERHWG+FY DG+PKY L +  S NGL+PA +V YLAKKWC
Sbjct: 310 DAYLFGLIDEDAKSIQPGNFERHWGIFYIDGQPKYQLSL-GSGNGLIPAKDVHYLAKKWC 369

Query: 372 VLAPSASIDDPQIAPSVSYACENGDCTSLGLGTSCGTLDARQNISYAFNSYYQIKNQLAS 431
           +LAP+A++ DPQ+ PSVSYAC++ DCTSLG G+SCG L+  QN+SYAFNSYYQ+ NQL S
Sbjct: 370 ILAPNANLQDPQLGPSVSYACDHADCTSLGYGSSCGNLNLAQNVSYAFNSYYQVSNQLDS 429

Query: 432 ACKFPHLSVITTQDPSVGSCKFVVMTQPSEVA-VNEKSKALRHSAESLLFFLCLFIL 482
           ACKFP LS+++T+DPSVGSCKF +M +  + +  +      R +A  LL  +CL+I+
Sbjct: 430 ACKFPGLSIVSTRDPSVGSCKFKIMIKSEDASEASAMMPITRSTAVLLLLSICLYIV 483

BLAST of IVF0024251 vs. ExPASy Swiss-Prot
Match: Q93Z08 (Glucan endo-1,3-beta-glucosidase 6 OS=Arabidopsis thaliana OX=3702 GN=At5g58090 PE=2 SV=2)

HSP 1 Score: 605.9 bits (1561), Expect = 4.0e-172
Identity = 284/453 (62.69%), Postives = 358/453 (79.03%), Query Frame = 0

Query: 8   VSWRFFLVLLLSSAAVSSVIGIGVNWGTQATHPLPPGTVVKMLKDNGIQKVKLFDADPVV 67
           + W   L+LL  +        IG NWGTQA+HPLPP  VV+ML++NGIQKVKLFDA+   
Sbjct: 1   MGWGSVLLLLAVALLCQRASSIGANWGTQASHPLPPDIVVRMLRENGIQKVKLFDAEYDT 60

Query: 68  LKALGKSGIEVMVGIPNDLLYSLANSIQSAETGLLKTCLLIFHLAV------YVAVGNEP 127
           L+ALGKSGIEVMVGIPN++L +LA+S+++AE  + K   +  H++       YVAVGNEP
Sbjct: 61  LRALGKSGIEVMVGIPNEMLATLASSLKAAEKWVAKN--VSTHISTDNVNIRYVAVGNEP 120

Query: 128 FLKTFNGSFIGTTFPALQNVQAAIVKAGLSNRVKVTVPMNADVYQTSTGLPSGGDFRSDI 187
           FL T+NGS++ TTFPAL+N+Q AI+KAGL N+VKVT P+NADVY +ST  PSGGDFR++I
Sbjct: 121 FLSTYNGSYLSTTFPALRNIQIAIIKAGLQNQVKVTCPLNADVYDSSTTFPSGGDFRANI 180

Query: 188 RDLMLSIVKFLSDSASPFTVNIYPFISLYEDPSFPIDYAFFDGFSHALNDNGQIYENVFT 247
           RDLM++IVKFLS++  PFTVNIYP+ISLY +P FP+DYAFFDG +  LND G  Y N+F 
Sbjct: 181 RDLMITIVKFLSENGGPFTVNIYPYISLYTNPDFPVDYAFFDGNAQPLNDGGTFYYNMFD 240

Query: 248 ANYDTLVSSLQKNGFPNMSIIVGEIGWPTDGDRNANLQFAKRFSQGFISHFLSGQGTPMR 307
           ANYDTLV +L+KNGF NM II+GEIGWPTDGD NANL +AK+F+QGF++H   G+GTP R
Sbjct: 241 ANYDTLVHALEKNGFGNMPIIIGEIGWPTDGDSNANLDYAKKFNQGFMAHISGGKGTPRR 300

Query: 308 PGPIDVYLFSLIDEDAKSILPGNFERHWGLFYYDGRPKYMLQM-TNSTNGLVPASNVKYL 367
           PGPID YLFSLIDEDAKS+ PG FERHWG+F +DG PKY L + T +T  L+ A  V+YL
Sbjct: 301 PGPIDAYLFSLIDEDAKSVQPGYFERHWGIFTFDGLPKYALNLGTTNTGALIQAKGVRYL 360

Query: 368 AKKWCVLAPSASIDDPQIAPSVSYACENGDCTSLGLGTSCGTLDARQNISYAFNSYYQIK 427
            +KWCV+ P+  +DDPQ+AP+VSYAC  GDCTSLG+GTSC  LD +QNISYAFNSYYQI+
Sbjct: 361 ERKWCVMKPNVRLDDPQVAPAVSYACSLGDCTSLGVGTSCANLDGKQNISYAFNSYYQIQ 420

Query: 428 NQLASACKFPHLSVITTQDPSVGSCKFVVMTQP 454
           +QL +ACKFP++S +T  DPS G+C+F +M +P
Sbjct: 421 DQLDTACKFPNISEVTKTDPSTGTCRFPIMIEP 451

BLAST of IVF0024251 vs. ExPASy Swiss-Prot
Match: Q6NKW9 (Glucan endo-1,3-beta-glucosidase 8 OS=Arabidopsis thaliana OX=3702 GN=At1g64760 PE=2 SV=2)

HSP 1 Score: 450.3 bits (1157), Expect = 2.8e-125
Identity = 239/466 (51.29%), Postives = 307/466 (65.88%), Query Frame = 0

Query: 26  VIGIGVNWGTQATHPLPPGTVVKMLKDNGIQKVKLFDADPVVLKALGKSGIEVMVGIPND 85
           V G+GVNWGT ATH LPP TVV+MLKDN I KVKLFDAD   + AL  SG+EVMV IPND
Sbjct: 22  VNGLGVNWGTMATHKLPPKTVVQMLKDNNINKVKLFDADETTMGALAGSGLEVMVAIPND 81

Query: 86  LLYSLANSIQSAETGLLKTCLLIFHLAV---YVAVGNEPFLKTFNGSFIGTTFPALQNVQ 145
            L  + +  ++ +          F   V   +VAVGNEPFLK++NGSFI  TFPAL N+Q
Sbjct: 82  QLKVMTSYDRAKDWVRKNVTRYNFDGGVNITFVAVGNEPFLKSYNGSFINLTFPALANIQ 141

Query: 146 AAIVKAGLSNRVKVTVPMNADVYQT--STGLPSGGDFRSDIRDLMLSIVKFLSDSASPFT 205
            A+ +AGL N VK TVP+NADVY +  S  +PS G FR DI   M  IV FL  + +P T
Sbjct: 142 NALNEAGLGNSVKATVPLNADVYDSPASNPVPSAGRFRPDIIGQMTQIVDFLGKNNAPIT 201

Query: 206 VNIYPFISLYEDPSFPIDYAFFDGFSHALNDNGQIYENVFTANYDTLVSSLQKNGFPNMS 265
           +NIYPF+SLY +  FP++YAFFDG +  +NDNG  Y NVF AN+DTLVSSL+  G  +M 
Sbjct: 202 INIYPFLSLYGNDDFPLNYAFFDG-AEPINDNGIDYTNVFDANFDTLVSSLKAVGHGDMP 261

Query: 266 IIVGEIGWPTDGDRNANLQFAKRFSQGFISHFLSGQGTPMRPGPIDVYLFSLIDEDAKSI 325
           IIVGE+GWPT+GD++AN   A RF  G +    + +GTP+RP  I+VYLF L+DEDAKSI
Sbjct: 262 IIVGEVGWPTEGDKHANAGSAYRFYNGLLPRLGTNKGTPLRPTYIEVYLFGLLDEDAKSI 321

Query: 326 LPGNFERHWGLFYYDGRPKYMLQMT--NSTNGLVPASNVKYLAKKWCVLAPSASIDDPQI 385
            PG FERHWG+F +DG+PK+ + ++    +  L+ A NV YL  KWC   P A  D  ++
Sbjct: 322 APGPFERHWGIFKFDGQPKFPIDLSGQGQSKFLIGAQNVPYLPNKWCTFNPEAK-DLTKL 381

Query: 386 APSVSYACENGDCTSLGLGTSCGTLDARQNISYAFNSYYQIKNQLASACKFPHLSVITTQ 445
           A ++ YAC   DCT+LG G+SC TLDA  N SYAFN ++Q+KNQ  SAC F  L+ ITTQ
Sbjct: 382 AANIDYACTFSDCTALGYGSSCNTLDANGNASYAFNMFFQVKNQDESACYFQGLATITTQ 441

Query: 446 DPSVGSCKFVVMTQPSEVAVNEKSKALRHSAESLLFFLCLFILAVM 485
           + S G C F +        V   + +   S+ SL+  +  F+L+ M
Sbjct: 442 NISQGQCNFPIQ------IVASSASSFSCSSYSLVVLIVWFLLSGM 479

BLAST of IVF0024251 vs. ExPASy Swiss-Prot
Match: Q9FGH4 (Glucan endo-1,3-beta-glucosidase 9 OS=Arabidopsis thaliana OX=3702 GN=At5g58480 PE=2 SV=1)

HSP 1 Score: 348.6 bits (893), Expect = 1.1e-94
Identity = 189/453 (41.72%), Postives = 268/453 (59.16%), Query Frame = 0

Query: 11  RFFLVLLLSSAAVS----SVIGIGVNWGTQATHPLPPGTVVKMLKDNGIQKVKLFDADPV 70
           R FL+LL  +A +S    +V  +G+NWGT+A+HPLPP  VV++LK NGI KVKLFDADP 
Sbjct: 4   RLFLLLLAVTAGLSLTGTTVRAVGINWGTEASHPLPPSKVVELLKSNGIVKVKLFDADPK 63

Query: 71  VLKALGKSGIEVMVGIPNDLLYSLANSIQSAETGLLKTCLLIFH-----LAVYVAVGNEP 130
           VL+AL  S I V +GI N +L SL  S++ AE+ +       F+        YVAVG EP
Sbjct: 64  VLRALSGSNIGVTIGIQNSMLKSLNASVKVAESWVHDNVTRYFNGGNRVRIEYVAVGEEP 123

Query: 131 FLKTFNGSFIGTTFPALQNVQAAIVKAGLSNRVKVTVPMNADVYQTSTGLPSGGDFRSDI 190
           FL+++   +      A  N+Q A+VKA L+N VKV VP + D + + +G PS G FR+D+
Sbjct: 124 FLQSYGNQYKPFVIGAAMNIQNALVKANLANEVKVVVPSSFDSFLSESGRPSSGHFRADL 183

Query: 191 RDLMLSIVKFLSDSASPFTVNIYPFISLYEDPSFPIDYAFFDGFSHALNDNGQIYENVFT 250
              M+ ++ FL+   SPF V I PF+S +++ +  +D++ F   + A  D  + Y N F 
Sbjct: 184 NKTMIELLSFLTKHHSPFFVTISPFLSFHQNKNISLDFSLFKETAKAHKDGRKTYRNSFD 243

Query: 251 ANYDTLVSSLQKNGFPNMSIIVGEIGWPTDGDRNANLQFAKRFSQGFISHFLSGQGTPMR 310
            +YDTLVS+L   GF  + I+V +IGWPTDG  NA    A+ F +G I H      +  R
Sbjct: 244 LSYDTLVSALFTIGFSEVDIVVSKIGWPTDGAENATSLTAEAFFKGLIVHLEKKTASLPR 303

Query: 311 PGPIDVYLFSLIDEDAKSILPGNFERHWGLFYYDGRPKYMLQMTNSTNGLVPASNVKYLA 370
           P P++ Y+ SL+DED +++  GNFERHWG+F +DG+ KY     +     V A NV+YL 
Sbjct: 304 P-PVETYIESLLDEDQRNLSAGNFERHWGVFTFDGQAKYNFSFNHKNQ--VNAQNVQYLP 363

Query: 371 KKWCVLAPSASIDDPQIAPSVSYACENGDCTSLGLGTSCGTLDARQNISYAFNSYYQIKN 430
            KWCV+  +  + +   +     AC   DCTS+  G SC  +    N+SYAFNS YQ  +
Sbjct: 364 PKWCVVNNNKDLSN--ASARALEACAVADCTSILPGGSCSGIRWPGNVSYAFNSLYQQND 423

Query: 431 QLASACKFPHLSVITTQDPSVGSCKFVVMTQPS 455
             A +C F  L +ITT DPS  +C+F +    S
Sbjct: 424 HSAESCNFGGLGLITTVDPSEDNCRFSIQLDTS 451

BLAST of IVF0024251 vs. ExPASy Swiss-Prot
Match: Q9FJU9 (Glucan endo-1,3-beta-glucosidase 13 OS=Arabidopsis thaliana OX=3702 GN=At5g56590 PE=2 SV=1)

HSP 1 Score: 221.9 bits (564), Expect = 1.6e-56
Identity = 146/462 (31.60%), Postives = 238/462 (51.52%), Query Frame = 0

Query: 29  IGVNWGTQATHPLPPGTVVKMLKDNGIQKVKLFDADPVVLKALGKSGIEVMVGIPNDLLY 88
           +GV +G  A     P  VV++++ + I+ V+++D +  VLKA G + IE+M+G+PN  L 
Sbjct: 26  VGVCYGRSADDLPTPSKVVQLIQQHNIKYVRIYDYNSQVLKAFGNTSIELMIGVPNSDLN 85

Query: 89  SLANSIQSAETGLLKTCLLIFHLA--VYVAVG----NEPFLKTFNGSFIGTTFPALQNVQ 148
           + + S  + +T L  + L  +      Y+ VG    ++P +     SF+    PA+QNV 
Sbjct: 86  AFSQSQSNVDTWLKNSVLPYYPTTKITYITVGAESTDDPHINA--SSFV---VPAMQNVL 145

Query: 149 AAIVKAGLSNRVKVTVPMNADVYQTSTGLPSGGDFRSDIRDLMLSIVKFLSDSASPFTVN 208
            A+ K GLS R+KV+  ++  +   S   PS G F S     +  +++FL+++ SPF ++
Sbjct: 146 TALRKVGLSRRIKVSTTLSLGILSRSFP-PSAGAFNSSYAYFLRPMLEFLAENKSPFMID 205

Query: 209 IYPFISLYEDP-SFPIDYAFFDGFSHALNDN-GQIYENVFTANYDTLVSSLQKNGFPNMS 268
           +YP+ +  + P +  +DY  F+  S  ++ N G +Y+N+F A  D L  +L    F  + 
Sbjct: 206 LYPYYAYRDSPNNVSLDYVLFESSSEVIDPNTGLLYKNMFDAQVDALYYALTALNFRTIK 265

Query: 269 IIVGEIGWPTDGDRN----ANLQFAKRFSQGFISHFLSGQGTPMRPG-PIDVYLFSLIDE 328
           I+V E GWPT G       A+   A+ ++   I H ++ QGTP +PG  ++VY+FSL +E
Sbjct: 266 IMVTETGWPTKGSPKEKAAASSDNAETYNSNIIRHVVTNQGTPAKPGEAMNVYIFSLFNE 325

Query: 329 DAKSILPGNFERHWGLFYYDGRPKYMLQMTNSTNGL---VPASNVKYLAKKWCVLAPSAS 388
           + K+ L  + ER+WGLFY D    Y L  T  +NG       +N    +  WC+ +  AS
Sbjct: 326 NRKAGL--DSERNWGLFYPDQTSVYQLDFTGKSNGFHSNSSGTNSSGSSNSWCIASSKAS 385

Query: 389 IDDPQIAPSVSYACENG--DCTSLGLGTSCGTLDAR-QNISYAFNSYYQIKNQLASACKF 448
             D  +  ++ +AC  G  DCT++     C   D    + S+ FNSY+Q       AC F
Sbjct: 386 ERD--LKGALDWACGPGNVDCTAIQPSQPCFQPDTLVSHASFVFNSYFQQNRATDVACSF 445

Query: 449 PHLSVITTQDPSVGSCKFVVMTQPSEVAVNEKSKALRHSAES 472
               V   +DPS   C ++        A N  + AL  SA +
Sbjct: 446 GGAGVKVNKDPSYDKCIYITAGGNKTKATN--ATALTSSAST 475

BLAST of IVF0024251 vs. ExPASy TrEMBL
Match: A0A5D3E757 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G007090 PE=3 SV=1)

HSP 1 Score: 931.0 bits (2405), Expect = 2.0e-267
Identity = 470/488 (96.31%), Postives = 471/488 (96.52%), Query Frame = 0

Query: 1   MKSLKFVVSWRFFLVLLLSSAAVSSVIGIGVNWGTQATHPLPPGTVVKMLKDNGIQKVKL 60
           MKSLKFVVSWRFFLVLLLSSAAVSSVIGIGVNWGTQATHPLPPGTVVKMLKDNGIQKVKL
Sbjct: 1   MKSLKFVVSWRFFLVLLLSSAAVSSVIGIGVNWGTQATHPLPPGTVVKMLKDNGIQKVKL 60

Query: 61  FDADPVVLKALGKSGIEVMVGIPNDLLYSLANSIQSAETGLLKTCLLIFHLAV----YVA 120
           FDADPVVLKALGKSGIEVMVGIPNDLLYSLANSIQSAE  + K              YVA
Sbjct: 61  FDADPVVLKALGKSGIEVMVGIPNDLLYSLANSIQSAENWVAKNLSSYISSGSVDIRYVA 120

Query: 121 VGNEPFLKTFNGSFIGTTFPALQNVQAAIVKAGLSNRVKVTVPMNADVYQTSTGLPSGGD 180
           VGNEPFLKTFNGSFIGTTFPALQNVQAAIVKAGLSNRVKVTVPMNADVYQTSTGLPSGGD
Sbjct: 121 VGNEPFLKTFNGSFIGTTFPALQNVQAAIVKAGLSNRVKVTVPMNADVYQTSTGLPSGGD 180

Query: 181 FRSDIRDLMLSIVKFLSDSASPFTVNIYPFISLYEDPSFPIDYAFFDGFSHALNDNGQIY 240
           FRSDIRDLMLSIVKFLSDSASPFTVNIYPFISLYEDPSFPIDYAFFDGFSHALNDNGQIY
Sbjct: 181 FRSDIRDLMLSIVKFLSDSASPFTVNIYPFISLYEDPSFPIDYAFFDGFSHALNDNGQIY 240

Query: 241 ENVFTANYDTLVSSLQKNGFPNMSIIVGEIGWPTDGDRNANLQFAKRFSQGFISHFLSGQ 300
           ENVFTANYDTLVSSLQKNGFPNMSIIVGEIGWPTDGDRNANLQFAKRFSQGFISHFLSGQ
Sbjct: 241 ENVFTANYDTLVSSLQKNGFPNMSIIVGEIGWPTDGDRNANLQFAKRFSQGFISHFLSGQ 300

Query: 301 GTPMRPGPIDVYLFSLIDEDAKSILPGNFERHWGLFYYDGRPKYMLQMTNSTNGLVPASN 360
           GTPMRPGPIDVYLFSLIDEDAKSILPGNFERHWGLFYYDGRPKYMLQMTNSTNGLVPASN
Sbjct: 301 GTPMRPGPIDVYLFSLIDEDAKSILPGNFERHWGLFYYDGRPKYMLQMTNSTNGLVPASN 360

Query: 361 VKYLAKKWCVLAPSASIDDPQIAPSVSYACENGDCTSLGLGTSCGTLDARQNISYAFNSY 420
           VKYLAKKWCVLAPSASIDDPQIAPSVSYACENGDCTSLGLGTSCGTLDARQNISYAFNSY
Sbjct: 361 VKYLAKKWCVLAPSASIDDPQIAPSVSYACENGDCTSLGLGTSCGTLDARQNISYAFNSY 420

Query: 421 YQIKNQLASACKFPHLSVITTQDPSVGSCKFVVMTQPSEVAVNEKSKALRHSAESLLFFL 480
           YQIKNQLASACKFPHLSVITTQDPSVGSCKFVVMTQPSEVAVNEKSKALRHSAESLLFFL
Sbjct: 421 YQIKNQLASACKFPHLSVITTQDPSVGSCKFVVMTQPSEVAVNEKSKALRHSAESLLFFL 480

Query: 481 CLFILAVM 485
           CLFILAVM
Sbjct: 481 CLFILAVM 488

BLAST of IVF0024251 vs. ExPASy TrEMBL
Match: A0A1S3CR17 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103503788 PE=3 SV=1)

HSP 1 Score: 931.0 bits (2405), Expect = 2.0e-267
Identity = 470/488 (96.31%), Postives = 471/488 (96.52%), Query Frame = 0

Query: 1   MKSLKFVVSWRFFLVLLLSSAAVSSVIGIGVNWGTQATHPLPPGTVVKMLKDNGIQKVKL 60
           MKSLKFVVSWRFFLVLLLSSAAVSSVIGIGVNWGTQATHPLPPGTVVKMLKDNGIQKVKL
Sbjct: 1   MKSLKFVVSWRFFLVLLLSSAAVSSVIGIGVNWGTQATHPLPPGTVVKMLKDNGIQKVKL 60

Query: 61  FDADPVVLKALGKSGIEVMVGIPNDLLYSLANSIQSAETGLLKTCLLIFHLAV----YVA 120
           FDADPVVLKALGKSGIEVMVGIPNDLLYSLANSIQSAE  + K              YVA
Sbjct: 61  FDADPVVLKALGKSGIEVMVGIPNDLLYSLANSIQSAENWVAKNLSSYISSGSVDIRYVA 120

Query: 121 VGNEPFLKTFNGSFIGTTFPALQNVQAAIVKAGLSNRVKVTVPMNADVYQTSTGLPSGGD 180
           VGNEPFLKTFNGSFIGTTFPALQNVQAAIVKAGLSNRVKVTVPMNADVYQTSTGLPSGGD
Sbjct: 121 VGNEPFLKTFNGSFIGTTFPALQNVQAAIVKAGLSNRVKVTVPMNADVYQTSTGLPSGGD 180

Query: 181 FRSDIRDLMLSIVKFLSDSASPFTVNIYPFISLYEDPSFPIDYAFFDGFSHALNDNGQIY 240
           FRSDIRDLMLSIVKFLSDSASPFTVNIYPFISLYEDPSFPIDYAFFDGFSHALNDNGQIY
Sbjct: 181 FRSDIRDLMLSIVKFLSDSASPFTVNIYPFISLYEDPSFPIDYAFFDGFSHALNDNGQIY 240

Query: 241 ENVFTANYDTLVSSLQKNGFPNMSIIVGEIGWPTDGDRNANLQFAKRFSQGFISHFLSGQ 300
           ENVFTANYDTLVSSLQKNGFPNMSIIVGEIGWPTDGDRNANLQFAKRFSQGFISHFLSGQ
Sbjct: 241 ENVFTANYDTLVSSLQKNGFPNMSIIVGEIGWPTDGDRNANLQFAKRFSQGFISHFLSGQ 300

Query: 301 GTPMRPGPIDVYLFSLIDEDAKSILPGNFERHWGLFYYDGRPKYMLQMTNSTNGLVPASN 360
           GTPMRPGPIDVYLFSLIDEDAKSILPGNFERHWGLFYYDGRPKYMLQMTNSTNGLVPASN
Sbjct: 301 GTPMRPGPIDVYLFSLIDEDAKSILPGNFERHWGLFYYDGRPKYMLQMTNSTNGLVPASN 360

Query: 361 VKYLAKKWCVLAPSASIDDPQIAPSVSYACENGDCTSLGLGTSCGTLDARQNISYAFNSY 420
           VKYLAKKWCVLAPSASIDDPQIAPSVSYACENGDCTSLGLGTSCGTLDARQNISYAFNSY
Sbjct: 361 VKYLAKKWCVLAPSASIDDPQIAPSVSYACENGDCTSLGLGTSCGTLDARQNISYAFNSY 420

Query: 421 YQIKNQLASACKFPHLSVITTQDPSVGSCKFVVMTQPSEVAVNEKSKALRHSAESLLFFL 480
           YQIKNQLASACKFPHLSVITTQDPSVGSCKFVVMTQPSEVAVNEKSKALRHSAESLLFFL
Sbjct: 421 YQIKNQLASACKFPHLSVITTQDPSVGSCKFVVMTQPSEVAVNEKSKALRHSAESLLFFL 480

Query: 481 CLFILAVM 485
           CLFILAVM
Sbjct: 481 CLFILAVM 488

BLAST of IVF0024251 vs. ExPASy TrEMBL
Match: A0A0A0LGR2 ((1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_3G880540 PE=3 SV=1)

HSP 1 Score: 902.1 bits (2330), Expect = 9.9e-259
Identity = 450/488 (92.21%), Postives = 464/488 (95.08%), Query Frame = 0

Query: 1   MKSLKFVVSWRFFLVLLLSSAAVSSVIGIGVNWGTQATHPLPPGTVVKMLKDNGIQKVKL 60
           M+SLKFV +W FFL LL+SSAAVSSVIGIGVNWGTQATHPLPP TVVK+L+DNGIQKVKL
Sbjct: 1   MESLKFVAAWGFFLALLISSAAVSSVIGIGVNWGTQATHPLPPVTVVKLLQDNGIQKVKL 60

Query: 61  FDADPVVLKALGKSGIEVMVGIPNDLLYSLANSIQSAETGLLKTCLLIFHLAV----YVA 120
           FDADPVVLKALGKSGIEVMVGIPND+LYSLANS+QSAE  + K              YVA
Sbjct: 61  FDADPVVLKALGKSGIEVMVGIPNDMLYSLANSMQSAENWVSKNLSSYISSGSVDIRYVA 120

Query: 121 VGNEPFLKTFNGSFIGTTFPALQNVQAAIVKAGLSNRVKVTVPMNADVYQTSTGLPSGGD 180
           VGNEPFLKTFNGSFIGTTFPALQNVQAAIVKAGLSNRVKVTVPMNADVYQTSTGLPSGGD
Sbjct: 121 VGNEPFLKTFNGSFIGTTFPALQNVQAAIVKAGLSNRVKVTVPMNADVYQTSTGLPSGGD 180

Query: 181 FRSDIRDLMLSIVKFLSDSASPFTVNIYPFISLYEDPSFPIDYAFFDGFSHALNDNGQIY 240
           FRSDIRDLMLSIVKFLSDSASPFTVNIYPFISLYEDPSFPIDYAFFDGFSHALND G++Y
Sbjct: 181 FRSDIRDLMLSIVKFLSDSASPFTVNIYPFISLYEDPSFPIDYAFFDGFSHALNDEGRLY 240

Query: 241 ENVFTANYDTLVSSLQKNGFPNMSIIVGEIGWPTDGDRNANLQFAKRFSQGFISHFLSGQ 300
           ENVFTANYDTLVSSLQKNGFPNMSIIVGEIGWPTDGDRNANLQFAKRFSQGFISHF+SGQ
Sbjct: 241 ENVFTANYDTLVSSLQKNGFPNMSIIVGEIGWPTDGDRNANLQFAKRFSQGFISHFVSGQ 300

Query: 301 GTPMRPGPIDVYLFSLIDEDAKSILPGNFERHWGLFYYDGRPKYMLQMTNSTNGLVPASN 360
           GTPMRPGPIDVYLFSLIDEDAKSILPGNFERHWGLFYYDGRPKYMLQMTN+TNGLVPASN
Sbjct: 301 GTPMRPGPIDVYLFSLIDEDAKSILPGNFERHWGLFYYDGRPKYMLQMTNNTNGLVPASN 360

Query: 361 VKYLAKKWCVLAPSASIDDPQIAPSVSYACENGDCTSLGLGTSCGTLDARQNISYAFNSY 420
           VKYLAKKWCVLAPSASIDDPQIAPSVSYACENGDCTSLGLGTSCGTLD RQNISYAFNSY
Sbjct: 361 VKYLAKKWCVLAPSASIDDPQIAPSVSYACENGDCTSLGLGTSCGTLDVRQNISYAFNSY 420

Query: 421 YQIKNQLASACKFPHLSVITTQDPSVGSCKFVVMTQPSEVAVNEKSKALRHSAESLLFFL 480
           YQIKNQLASACKFPHLSV+T QDPSVGSCKF+VMTQPSEVAVNEKSKALRHSAESLLFFL
Sbjct: 421 YQIKNQLASACKFPHLSVVTAQDPSVGSCKFIVMTQPSEVAVNEKSKALRHSAESLLFFL 480

Query: 481 CLFILAVM 485
           CLFILAVM
Sbjct: 481 CLFILAVM 488

BLAST of IVF0024251 vs. ExPASy TrEMBL
Match: A0A6J1J195 ((1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111480957 PE=3 SV=1)

HSP 1 Score: 829.3 bits (2141), Expect = 8.1e-237
Identity = 415/488 (85.04%), Postives = 443/488 (90.78%), Query Frame = 0

Query: 1   MKSLKFVVSWRFFLVLLLSSAAVSSVIGIGVNWGTQATHPLPPGTVVKMLKDNGIQKVKL 60
           M+SLKF+     FLVLLLSSAAVSSVIGIGVNWGTQ+THPLPP TVVK+LKDNGIQKVKL
Sbjct: 1   MESLKFLAVRGLFLVLLLSSAAVSSVIGIGVNWGTQSTHPLPPATVVKLLKDNGIQKVKL 60

Query: 61  FDADPVVLKALGKSGIEVMVGIPNDLLYSLANSIQSAETGLLKT-CLLIFHLAV---YVA 120
           FDADPVVL AL KSGIEVMVGIPND+LYSLANSIQ AE  + K     I   +V   YVA
Sbjct: 61  FDADPVVLNALAKSGIEVMVGIPNDMLYSLANSIQDAENWVAKNLSSYISSTSVDIRYVA 120

Query: 121 VGNEPFLKTFNGSFIGTTFPALQNVQAAIVKAGLSNRVKVTVPMNADVYQTSTGLPSGGD 180
           VGNEPFLKTFNGSF+GTTFPALQN+QAAIVKAGLS RVKVTVPMNADVYQ+ T +PSGGD
Sbjct: 121 VGNEPFLKTFNGSFLGTTFPALQNIQAAIVKAGLSTRVKVTVPMNADVYQSLTTVPSGGD 180

Query: 181 FRSDIRDLMLSIVKFLSDSASPFTVNIYPFISLYEDPSFPIDYAFFDGFSHALNDNGQIY 240
           FRSDI DLMLSIVKFLSDS SPFTVNIYPFISLY DP+FPIDYAFFDGFS AL+DNG++Y
Sbjct: 181 FRSDIHDLMLSIVKFLSDSESPFTVNIYPFISLYADPNFPIDYAFFDGFSQALDDNGRVY 240

Query: 241 ENVFTANYDTLVSSLQKNGFPNMSIIVGEIGWPTDGDRNANLQFAKRFSQGFISHFLSGQ 300
           +NVFTANYDTLVSSLQKNGFPNMSIIVGEIGWPTDGDRNANLQFAKRFSQGF+S F+SGQ
Sbjct: 241 DNVFTANYDTLVSSLQKNGFPNMSIIVGEIGWPTDGDRNANLQFAKRFSQGFLSRFVSGQ 300

Query: 301 GTPMRPGPIDVYLFSLIDEDAKSILPGNFERHWGLFYYDGRPKYMLQMTNSTNGLVPASN 360
           GTPMRPGPID YLFSLIDEDAKSI PGNFERHWGLFYYDGRPKYMLQM N T  LVPASN
Sbjct: 301 GTPMRPGPIDAYLFSLIDEDAKSIQPGNFERHWGLFYYDGRPKYMLQMANGTRSLVPASN 360

Query: 361 VKYLAKKWCVLAPSASIDDPQIAPSVSYACENGDCTSLGLGTSCGTLDARQNISYAFNSY 420
           VKY AK+WCVL+PSAS+DDPQIAPSVSYACEN DCTSLG  TSCG LDARQNISYAFNSY
Sbjct: 361 VKYQAKQWCVLSPSASLDDPQIAPSVSYACENSDCTSLGFQTSCGALDARQNISYAFNSY 420

Query: 421 YQIKNQLASACKFPHLSVITTQDPSVGSCKFVVMTQPSEVAVNEKSKALRHSAESLLFFL 480
           YQIKNQ+AS+CKFPHLSVI+TQDPSVGSC+F+VMTQPSEVAVNEKSKALR+SA SLL FL
Sbjct: 421 YQIKNQVASSCKFPHLSVISTQDPSVGSCRFIVMTQPSEVAVNEKSKALRYSAGSLLLFL 480

Query: 481 CLFILAVM 485
           C FILAV+
Sbjct: 481 CFFILAVL 488

BLAST of IVF0024251 vs. ExPASy TrEMBL
Match: A0A6J1FPD1 ((1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111446059 PE=3 SV=1)

HSP 1 Score: 828.9 bits (2140), Expect = 1.1e-236
Identity = 415/488 (85.04%), Postives = 444/488 (90.98%), Query Frame = 0

Query: 1   MKSLKFVVSWRFFLVLLLSSAAVSSVIGIGVNWGTQATHPLPPGTVVKMLKDNGIQKVKL 60
           M+SLKF+     FLVLLLSSAAVSSVIGIGVNWGTQ+THPLPP TVVK+LKDNGIQKVKL
Sbjct: 39  MESLKFLAVRGLFLVLLLSSAAVSSVIGIGVNWGTQSTHPLPPATVVKLLKDNGIQKVKL 98

Query: 61  FDADPVVLKALGKSGIEVMVGIPNDLLYSLANSIQSAETGLLKT-CLLIFHLAV---YVA 120
           FDADPVVL AL KSGIEVMVGIPND+LYSLANSIQ+AE  + K     I   +V   YVA
Sbjct: 99  FDADPVVLNALAKSGIEVMVGIPNDMLYSLANSIQAAENWVAKNLSSYISSTSVDIRYVA 158

Query: 121 VGNEPFLKTFNGSFIGTTFPALQNVQAAIVKAGLSNRVKVTVPMNADVYQTSTGLPSGGD 180
           VGNEPFLKTFNGSF+GTTFPALQNVQAAIVKAGLS RVKVTVPMNADVYQ+ T +PSGGD
Sbjct: 159 VGNEPFLKTFNGSFLGTTFPALQNVQAAIVKAGLSTRVKVTVPMNADVYQSLTTVPSGGD 218

Query: 181 FRSDIRDLMLSIVKFLSDSASPFTVNIYPFISLYEDPSFPIDYAFFDGFSHALNDNGQIY 240
           FRSDI DLMLSIVKFLSDS SPFTVNIYPFISLY DP+FPIDYAFFDGFS AL+DNG++Y
Sbjct: 219 FRSDIHDLMLSIVKFLSDSESPFTVNIYPFISLYADPNFPIDYAFFDGFSQALDDNGRVY 278

Query: 241 ENVFTANYDTLVSSLQKNGFPNMSIIVGEIGWPTDGDRNANLQFAKRFSQGFISHFLSGQ 300
           +NVFTANYDTLVSSLQKNGFPNMSIIVGEIGWPTDGDRNANLQFAKRFSQGF+S F+SGQ
Sbjct: 279 DNVFTANYDTLVSSLQKNGFPNMSIIVGEIGWPTDGDRNANLQFAKRFSQGFLSRFVSGQ 338

Query: 301 GTPMRPGPIDVYLFSLIDEDAKSILPGNFERHWGLFYYDGRPKYMLQMTNSTNGLVPASN 360
           GTPMRPGPID YLFSLIDEDAKSI PGNFERHWGLFYYDGRPKYMLQM N T  LVPASN
Sbjct: 339 GTPMRPGPIDAYLFSLIDEDAKSIQPGNFERHWGLFYYDGRPKYMLQMANGTRSLVPASN 398

Query: 361 VKYLAKKWCVLAPSASIDDPQIAPSVSYACENGDCTSLGLGTSCGTLDARQNISYAFNSY 420
           VKY AK+WCVL+PSAS+DDPQIAPSVSYACEN DCTSLG  TSCG LDARQNISYAFNSY
Sbjct: 399 VKYQAKQWCVLSPSASLDDPQIAPSVSYACENSDCTSLGFQTSCGALDARQNISYAFNSY 458

Query: 421 YQIKNQLASACKFPHLSVITTQDPSVGSCKFVVMTQPSEVAVNEKSKALRHSAESLLFFL 480
           YQIKNQ+AS+CKFPHLSVI+TQDPSVGSC+F+VMTQPSEVAVNEKSKALR+SA SLL FL
Sbjct: 459 YQIKNQVASSCKFPHLSVISTQDPSVGSCRFIVMTQPSEVAVNEKSKALRYSAGSLLLFL 518

Query: 481 CLFILAVM 485
           C FILA++
Sbjct: 519 CFFILALL 526

BLAST of IVF0024251 vs. NCBI nr
Match: XP_008466353.1 (PREDICTED: glucan endo-1,3-beta-glucosidase 5 [Cucumis melo] >KAA0038781.1 glucan endo-1,3-beta-glucosidase 5 [Cucumis melo var. makuwa] >TYK31401.1 glucan endo-1,3-beta-glucosidase 5 [Cucumis melo var. makuwa])

HSP 1 Score: 923 bits (2386), Expect = 0.0
Identity = 470/488 (96.31%), Postives = 471/488 (96.52%), Query Frame = 0

Query: 1   MKSLKFVVSWRFFLVLLLSSAAVSSVIGIGVNWGTQATHPLPPGTVVKMLKDNGIQKVKL 60
           MKSLKFVVSWRFFLVLLLSSAAVSSVIGIGVNWGTQATHPLPPGTVVKMLKDNGIQKVKL
Sbjct: 1   MKSLKFVVSWRFFLVLLLSSAAVSSVIGIGVNWGTQATHPLPPGTVVKMLKDNGIQKVKL 60

Query: 61  FDADPVVLKALGKSGIEVMVGIPNDLLYSLANSIQSAETGLLKTCLLIFHLAV----YVA 120
           FDADPVVLKALGKSGIEVMVGIPNDLLYSLANSIQSAE  + K              YVA
Sbjct: 61  FDADPVVLKALGKSGIEVMVGIPNDLLYSLANSIQSAENWVAKNLSSYISSGSVDIRYVA 120

Query: 121 VGNEPFLKTFNGSFIGTTFPALQNVQAAIVKAGLSNRVKVTVPMNADVYQTSTGLPSGGD 180
           VGNEPFLKTFNGSFIGTTFPALQNVQAAIVKAGLSNRVKVTVPMNADVYQTSTGLPSGGD
Sbjct: 121 VGNEPFLKTFNGSFIGTTFPALQNVQAAIVKAGLSNRVKVTVPMNADVYQTSTGLPSGGD 180

Query: 181 FRSDIRDLMLSIVKFLSDSASPFTVNIYPFISLYEDPSFPIDYAFFDGFSHALNDNGQIY 240
           FRSDIRDLMLSIVKFLSDSASPFTVNIYPFISLYEDPSFPIDYAFFDGFSHALNDNGQIY
Sbjct: 181 FRSDIRDLMLSIVKFLSDSASPFTVNIYPFISLYEDPSFPIDYAFFDGFSHALNDNGQIY 240

Query: 241 ENVFTANYDTLVSSLQKNGFPNMSIIVGEIGWPTDGDRNANLQFAKRFSQGFISHFLSGQ 300
           ENVFTANYDTLVSSLQKNGFPNMSIIVGEIGWPTDGDRNANLQFAKRFSQGFISHFLSGQ
Sbjct: 241 ENVFTANYDTLVSSLQKNGFPNMSIIVGEIGWPTDGDRNANLQFAKRFSQGFISHFLSGQ 300

Query: 301 GTPMRPGPIDVYLFSLIDEDAKSILPGNFERHWGLFYYDGRPKYMLQMTNSTNGLVPASN 360
           GTPMRPGPIDVYLFSLIDEDAKSILPGNFERHWGLFYYDGRPKYMLQMTNSTNGLVPASN
Sbjct: 301 GTPMRPGPIDVYLFSLIDEDAKSILPGNFERHWGLFYYDGRPKYMLQMTNSTNGLVPASN 360

Query: 361 VKYLAKKWCVLAPSASIDDPQIAPSVSYACENGDCTSLGLGTSCGTLDARQNISYAFNSY 420
           VKYLAKKWCVLAPSASIDDPQIAPSVSYACENGDCTSLGLGTSCGTLDARQNISYAFNSY
Sbjct: 361 VKYLAKKWCVLAPSASIDDPQIAPSVSYACENGDCTSLGLGTSCGTLDARQNISYAFNSY 420

Query: 421 YQIKNQLASACKFPHLSVITTQDPSVGSCKFVVMTQPSEVAVNEKSKALRHSAESLLFFL 480
           YQIKNQLASACKFPHLSVITTQDPSVGSCKFVVMTQPSEVAVNEKSKALRHSAESLLFFL
Sbjct: 421 YQIKNQLASACKFPHLSVITTQDPSVGSCKFVVMTQPSEVAVNEKSKALRHSAESLLFFL 480

Query: 481 CLFILAVM 484
           CLFILAVM
Sbjct: 481 CLFILAVM 488

BLAST of IVF0024251 vs. NCBI nr
Match: XP_004136331.1 (glucan endo-1,3-beta-glucosidase 5 [Cucumis sativus] >KGN60139.1 hypothetical protein Csa_002300 [Cucumis sativus])

HSP 1 Score: 894 bits (2311), Expect = 0.0
Identity = 450/488 (92.21%), Postives = 464/488 (95.08%), Query Frame = 0

Query: 1   MKSLKFVVSWRFFLVLLLSSAAVSSVIGIGVNWGTQATHPLPPGTVVKMLKDNGIQKVKL 60
           M+SLKFV +W FFL LL+SSAAVSSVIGIGVNWGTQATHPLPP TVVK+L+DNGIQKVKL
Sbjct: 1   MESLKFVAAWGFFLALLISSAAVSSVIGIGVNWGTQATHPLPPVTVVKLLQDNGIQKVKL 60

Query: 61  FDADPVVLKALGKSGIEVMVGIPNDLLYSLANSIQSAETGLLKTCLLIFHLAV----YVA 120
           FDADPVVLKALGKSGIEVMVGIPND+LYSLANS+QSAE  + K              YVA
Sbjct: 61  FDADPVVLKALGKSGIEVMVGIPNDMLYSLANSMQSAENWVSKNLSSYISSGSVDIRYVA 120

Query: 121 VGNEPFLKTFNGSFIGTTFPALQNVQAAIVKAGLSNRVKVTVPMNADVYQTSTGLPSGGD 180
           VGNEPFLKTFNGSFIGTTFPALQNVQAAIVKAGLSNRVKVTVPMNADVYQTSTGLPSGGD
Sbjct: 121 VGNEPFLKTFNGSFIGTTFPALQNVQAAIVKAGLSNRVKVTVPMNADVYQTSTGLPSGGD 180

Query: 181 FRSDIRDLMLSIVKFLSDSASPFTVNIYPFISLYEDPSFPIDYAFFDGFSHALNDNGQIY 240
           FRSDIRDLMLSIVKFLSDSASPFTVNIYPFISLYEDPSFPIDYAFFDGFSHALND G++Y
Sbjct: 181 FRSDIRDLMLSIVKFLSDSASPFTVNIYPFISLYEDPSFPIDYAFFDGFSHALNDEGRLY 240

Query: 241 ENVFTANYDTLVSSLQKNGFPNMSIIVGEIGWPTDGDRNANLQFAKRFSQGFISHFLSGQ 300
           ENVFTANYDTLVSSLQKNGFPNMSIIVGEIGWPTDGDRNANLQFAKRFSQGFISHF+SGQ
Sbjct: 241 ENVFTANYDTLVSSLQKNGFPNMSIIVGEIGWPTDGDRNANLQFAKRFSQGFISHFVSGQ 300

Query: 301 GTPMRPGPIDVYLFSLIDEDAKSILPGNFERHWGLFYYDGRPKYMLQMTNSTNGLVPASN 360
           GTPMRPGPIDVYLFSLIDEDAKSILPGNFERHWGLFYYDGRPKYMLQMTN+TNGLVPASN
Sbjct: 301 GTPMRPGPIDVYLFSLIDEDAKSILPGNFERHWGLFYYDGRPKYMLQMTNNTNGLVPASN 360

Query: 361 VKYLAKKWCVLAPSASIDDPQIAPSVSYACENGDCTSLGLGTSCGTLDARQNISYAFNSY 420
           VKYLAKKWCVLAPSASIDDPQIAPSVSYACENGDCTSLGLGTSCGTLD RQNISYAFNSY
Sbjct: 361 VKYLAKKWCVLAPSASIDDPQIAPSVSYACENGDCTSLGLGTSCGTLDVRQNISYAFNSY 420

Query: 421 YQIKNQLASACKFPHLSVITTQDPSVGSCKFVVMTQPSEVAVNEKSKALRHSAESLLFFL 480
           YQIKNQLASACKFPHLSV+T QDPSVGSCKF+VMTQPSEVAVNEKSKALRHSAESLLFFL
Sbjct: 421 YQIKNQLASACKFPHLSVVTAQDPSVGSCKFIVMTQPSEVAVNEKSKALRHSAESLLFFL 480

Query: 481 CLFILAVM 484
           CLFILAVM
Sbjct: 481 CLFILAVM 488

BLAST of IVF0024251 vs. NCBI nr
Match: XP_038898735.1 (glucan endo-1,3-beta-glucosidase 5 [Benincasa hispida])

HSP 1 Score: 863 bits (2229), Expect = 4.72e-314
Identity = 435/485 (89.69%), Postives = 451/485 (92.99%), Query Frame = 0

Query: 4   LKFVVSWRFFLVLLLSSAAVSSVIGIGVNWGTQATHPLPPGTVVKMLKDNGIQKVKLFDA 63
           LKFV  W FFLVL LSSAAVSSVIGIGVNWGTQATHPLPP TVVK+LKDNGIQKVKLFDA
Sbjct: 4   LKFVAGWGFFLVLSLSSAAVSSVIGIGVNWGTQATHPLPPATVVKLLKDNGIQKVKLFDA 63

Query: 64  DPVVLKALGKSGIEVMVGIPNDLLYSLANSIQSAETGLLKTCLLIFHLAV----YVAVGN 123
           DPVVLKALGKSGIEVMVGIPND+LYSLANSIQSAE  + K              YVAVGN
Sbjct: 64  DPVVLKALGKSGIEVMVGIPNDMLYSLANSIQSAENWVAKNLSSYISSGSVDIRYVAVGN 123

Query: 124 EPFLKTFNGSFIGTTFPALQNVQAAIVKAGLSNRVKVTVPMNADVYQTSTGLPSGGDFRS 183
           EPFLKTFNGSF+GTTFPALQNVQAAIVKAGLSNRVKVTVPMNADVYQ+ TGLPSGGDFRS
Sbjct: 124 EPFLKTFNGSFLGTTFPALQNVQAAIVKAGLSNRVKVTVPMNADVYQSLTGLPSGGDFRS 183

Query: 184 DIRDLMLSIVKFLSDSASPFTVNIYPFISLYEDPSFPIDYAFFDGFSHALNDNGQIYENV 243
           DI DLMLSIVKFLSDSASPFTVNIYPFISLYEDP+FPID+AFFDGFS AL+DNG++YENV
Sbjct: 184 DIHDLMLSIVKFLSDSASPFTVNIYPFISLYEDPNFPIDFAFFDGFSSALSDNGRVYENV 243

Query: 244 FTANYDTLVSSLQKNGFPNMSIIVGEIGWPTDGDRNANLQFAKRFSQGFISHFLSGQGTP 303
           FTANYDTLVSSLQKNGFPNMSIIVGEIGWPTDGD NANLQFAKRFSQGFISHF+SGQGTP
Sbjct: 244 FTANYDTLVSSLQKNGFPNMSIIVGEIGWPTDGDWNANLQFAKRFSQGFISHFISGQGTP 303

Query: 304 MRPGPIDVYLFSLIDEDAKSILPGNFERHWGLFYYDGRPKYMLQMTNSTNGLVPASNVKY 363
           MRPGPIDVYLFSLIDEDAKSILPGNFERHWGLFYYDGRPKYMLQM N TNGLVPASNVKY
Sbjct: 304 MRPGPIDVYLFSLIDEDAKSILPGNFERHWGLFYYDGRPKYMLQMANGTNGLVPASNVKY 363

Query: 364 LAKKWCVLAPSASIDDPQIAPSVSYACENGDCTSLGLGTSCGTLDARQNISYAFNSYYQI 423
           LAK+WCVLAPSASIDDPQIAPSVSYACENGDCTSLGLGTSCG LDARQNISYAFNSYYQI
Sbjct: 364 LAKQWCVLAPSASIDDPQIAPSVSYACENGDCTSLGLGTSCGALDARQNISYAFNSYYQI 423

Query: 424 KNQLASACKFPHLSVITTQDPSVGSCKFVVMTQPSEVAVNEKSKALRHSAESLLFFLCLF 483
           KNQLASACKFPHLSVITTQDPSVGSCKF+VMTQPSEVAVNEKS+A  HSA SLL FLC F
Sbjct: 424 KNQLASACKFPHLSVITTQDPSVGSCKFIVMTQPSEVAVNEKSEAPSHSAGSLLLFLCSF 483

BLAST of IVF0024251 vs. NCBI nr
Match: XP_023524183.1 (glucan endo-1,3-beta-glucosidase 5 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 823 bits (2125), Expect = 3.33e-298
Identity = 416/488 (85.25%), Postives = 445/488 (91.19%), Query Frame = 0

Query: 1   MKSLKFVVSWRFFLVLLLSSAAVSSVIGIGVNWGTQATHPLPPGTVVKMLKDNGIQKVKL 60
           M+SLKF+V    FLVLLLSSAAVSSVIGIGVNWGTQ+THPLPP TVVK+LKDNGIQKVKL
Sbjct: 1   MESLKFLVVRGLFLVLLLSSAAVSSVIGIGVNWGTQSTHPLPPATVVKLLKDNGIQKVKL 60

Query: 61  FDADPVVLKALGKSGIEVMVGIPNDLLYSLANSIQSAETGLLKTCL-LIFHLAV---YVA 120
           FDADPVVL AL KSGIEVMVGIPND+LYSLANSIQ+AE  + K     I   +V   YVA
Sbjct: 61  FDADPVVLNALAKSGIEVMVGIPNDMLYSLANSIQAAENWVAKNLSSYISSTSVDIRYVA 120

Query: 121 VGNEPFLKTFNGSFIGTTFPALQNVQAAIVKAGLSNRVKVTVPMNADVYQTSTGLPSGGD 180
           VGNEPFLKTFNGSF+GTTFPALQNVQAAIVKAGLS RVKVTVPMNADVYQ+ T +PSGGD
Sbjct: 121 VGNEPFLKTFNGSFLGTTFPALQNVQAAIVKAGLSTRVKVTVPMNADVYQSLTTVPSGGD 180

Query: 181 FRSDIRDLMLSIVKFLSDSASPFTVNIYPFISLYEDPSFPIDYAFFDGFSHALNDNGQIY 240
           FRSDI DLMLSIVKFLSDS SPFTVNIYPFISLY DP+FPIDYAFFDGFS AL+DNG++Y
Sbjct: 181 FRSDIHDLMLSIVKFLSDSESPFTVNIYPFISLYADPNFPIDYAFFDGFSQALDDNGRVY 240

Query: 241 ENVFTANYDTLVSSLQKNGFPNMSIIVGEIGWPTDGDRNANLQFAKRFSQGFISHFLSGQ 300
           +NVFTANYDTLVSSLQKNGFPNMSIIVGEIGWPTDGDRNANLQFAKRFSQGF+S F+SGQ
Sbjct: 241 DNVFTANYDTLVSSLQKNGFPNMSIIVGEIGWPTDGDRNANLQFAKRFSQGFLSRFVSGQ 300

Query: 301 GTPMRPGPIDVYLFSLIDEDAKSILPGNFERHWGLFYYDGRPKYMLQMTNSTNGLVPASN 360
           GTPMRPGPID YLFSLIDEDAKSI PGNFERHWGLFYYDGRPKYMLQM N T  LVPASN
Sbjct: 301 GTPMRPGPIDAYLFSLIDEDAKSIQPGNFERHWGLFYYDGRPKYMLQMANGTRSLVPASN 360

Query: 361 VKYLAKKWCVLAPSASIDDPQIAPSVSYACENGDCTSLGLGTSCGTLDARQNISYAFNSY 420
           VKY AK+WCVL+PSAS+DDPQIAPSVSYACEN DCTSLG  TSCG LDARQNISYAFNSY
Sbjct: 361 VKYQAKQWCVLSPSASLDDPQIAPSVSYACENSDCTSLGFQTSCGALDARQNISYAFNSY 420

Query: 421 YQIKNQLASACKFPHLSVITTQDPSVGSCKFVVMTQPSEVAVNEKSKALRHSAESLLFFL 480
           YQIKNQ+AS+CKFPHLSVI+TQDPSVGSC+F+VMTQPSEVAVNEKSKALR+SA SLL FL
Sbjct: 421 YQIKNQVASSCKFPHLSVISTQDPSVGSCRFIVMTQPSEVAVNEKSKALRYSAGSLLLFL 480

Query: 481 CLFILAVM 484
           C FIL+V+
Sbjct: 481 CFFILSVL 488

BLAST of IVF0024251 vs. NCBI nr
Match: XP_022981965.1 (glucan endo-1,3-beta-glucosidase 5 [Cucurbita maxima])

HSP 1 Score: 822 bits (2122), Expect = 9.53e-298
Identity = 415/488 (85.04%), Postives = 443/488 (90.78%), Query Frame = 0

Query: 1   MKSLKFVVSWRFFLVLLLSSAAVSSVIGIGVNWGTQATHPLPPGTVVKMLKDNGIQKVKL 60
           M+SLKF+     FLVLLLSSAAVSSVIGIGVNWGTQ+THPLPP TVVK+LKDNGIQKVKL
Sbjct: 1   MESLKFLAVRGLFLVLLLSSAAVSSVIGIGVNWGTQSTHPLPPATVVKLLKDNGIQKVKL 60

Query: 61  FDADPVVLKALGKSGIEVMVGIPNDLLYSLANSIQSAETGLLKTCL-LIFHLAV---YVA 120
           FDADPVVL AL KSGIEVMVGIPND+LYSLANSIQ AE  + K     I   +V   YVA
Sbjct: 61  FDADPVVLNALAKSGIEVMVGIPNDMLYSLANSIQDAENWVAKNLSSYISSTSVDIRYVA 120

Query: 121 VGNEPFLKTFNGSFIGTTFPALQNVQAAIVKAGLSNRVKVTVPMNADVYQTSTGLPSGGD 180
           VGNEPFLKTFNGSF+GTTFPALQN+QAAIVKAGLS RVKVTVPMNADVYQ+ T +PSGGD
Sbjct: 121 VGNEPFLKTFNGSFLGTTFPALQNIQAAIVKAGLSTRVKVTVPMNADVYQSLTTVPSGGD 180

Query: 181 FRSDIRDLMLSIVKFLSDSASPFTVNIYPFISLYEDPSFPIDYAFFDGFSHALNDNGQIY 240
           FRSDI DLMLSIVKFLSDS SPFTVNIYPFISLY DP+FPIDYAFFDGFS AL+DNG++Y
Sbjct: 181 FRSDIHDLMLSIVKFLSDSESPFTVNIYPFISLYADPNFPIDYAFFDGFSQALDDNGRVY 240

Query: 241 ENVFTANYDTLVSSLQKNGFPNMSIIVGEIGWPTDGDRNANLQFAKRFSQGFISHFLSGQ 300
           +NVFTANYDTLVSSLQKNGFPNMSIIVGEIGWPTDGDRNANLQFAKRFSQGF+S F+SGQ
Sbjct: 241 DNVFTANYDTLVSSLQKNGFPNMSIIVGEIGWPTDGDRNANLQFAKRFSQGFLSRFVSGQ 300

Query: 301 GTPMRPGPIDVYLFSLIDEDAKSILPGNFERHWGLFYYDGRPKYMLQMTNSTNGLVPASN 360
           GTPMRPGPID YLFSLIDEDAKSI PGNFERHWGLFYYDGRPKYMLQM N T  LVPASN
Sbjct: 301 GTPMRPGPIDAYLFSLIDEDAKSIQPGNFERHWGLFYYDGRPKYMLQMANGTRSLVPASN 360

Query: 361 VKYLAKKWCVLAPSASIDDPQIAPSVSYACENGDCTSLGLGTSCGTLDARQNISYAFNSY 420
           VKY AK+WCVL+PSAS+DDPQIAPSVSYACEN DCTSLG  TSCG LDARQNISYAFNSY
Sbjct: 361 VKYQAKQWCVLSPSASLDDPQIAPSVSYACENSDCTSLGFQTSCGALDARQNISYAFNSY 420

Query: 421 YQIKNQLASACKFPHLSVITTQDPSVGSCKFVVMTQPSEVAVNEKSKALRHSAESLLFFL 480
           YQIKNQ+AS+CKFPHLSVI+TQDPSVGSC+F+VMTQPSEVAVNEKSKALR+SA SLL FL
Sbjct: 421 YQIKNQVASSCKFPHLSVISTQDPSVGSCRFIVMTQPSEVAVNEKSKALRYSAGSLLLFL 480

Query: 481 CLFILAVM 484
           C FILAV+
Sbjct: 481 CFFILAVL 488

BLAST of IVF0024251 vs. TAIR 10
Match: AT4G31140.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 627.5 bits (1617), Expect = 9.0e-180
Identity = 298/477 (62.47%), Postives = 384/477 (80.50%), Query Frame = 0

Query: 12  FFLVLLLSSAAVSSVIGIGVNWGTQATHPLPPGTVVKMLKDNGIQKVKLFDADPVVLKAL 71
           FF++ LL ++ +  V GIGVNWG+QA HPLPP TVV++L++NGIQKVKLF+AD  +LKAL
Sbjct: 10  FFVITLLYASLLIEVEGIGVNWGSQARHPLPPATVVRLLRENGIQKVKLFEADSAILKAL 69

Query: 72  GKSGIEVMVGIPNDLLYSLANSIQSAETGLLKTCLLIFHLAV------YVAVGNEPFLKT 131
            ++GI+VMVGIPNDLL  LA S+ +AE  + +   +  H++       YVAVGNEPFLK 
Sbjct: 70  SRTGIQVMVGIPNDLLAPLAGSVAAAERWVSQN--VSAHVSSNGVDIRYVAVGNEPFLKA 129

Query: 132 FNGSFIGTTFPALQNVQAAIVKAGLSNRVKVTVPMNADVYQTSTGLPSGGDFRSDIRDLM 191
           FNG+F G T PALQN+Q+AI+KAGL+ +VKVTVP+NADVYQ+++ LPS GDFR +IRDLM
Sbjct: 130 FNGTFEGITLPALQNIQSAIIKAGLATQVKVTVPLNADVYQSASNLPSDGDFRPEIRDLM 189

Query: 192 LSIVKFLSDSASPFTVNIYPFISLYEDPSFPIDYAFFDGFSHALNDNGQIYENVFTANYD 251
           L+IVKFLSD+ +PFT+NIYPFISLY DP+FP+++AFFDG    +NDNG+IY+NV  ANYD
Sbjct: 190 LNIVKFLSDNGAPFTINIYPFISLYNDPNFPVEFAFFDGTGTPINDNGRIYDNVLDANYD 249

Query: 252 TLVSSLQKNGFPNMSIIVGEIGWPTDGDRNANLQFAKRFSQGFISHFLSGQGTPMRPGPI 311
           TLV SLQKNGF N++IIVGE+GWPTDGD+NANL +A+R++QGF++   + +GTPMRPG +
Sbjct: 250 TLVWSLQKNGFGNLTIIVGEVGWPTDGDKNANLMYARRYNQGFMNRQKANKGTPMRPGAM 309

Query: 312 DVYLFSLIDEDAKSILPGNFERHWGLFYYDGRPKYMLQMTNSTNGLVPASNVKYLAKKWC 371
           D YLF LIDEDAKSI PGNFERHWG+FY DG+PKY L +  S NGL+PA +V YLAKKWC
Sbjct: 310 DAYLFGLIDEDAKSIQPGNFERHWGIFYIDGQPKYQLSL-GSGNGLIPAKDVHYLAKKWC 369

Query: 372 VLAPSASIDDPQIAPSVSYACENGDCTSLGLGTSCGTLDARQNISYAFNSYYQIKNQLAS 431
           +LAP+A++ DPQ+ PSVSYAC++ DCTSLG G+SCG L+  QN+SYAFNSYYQ+ NQL S
Sbjct: 370 ILAPNANLQDPQLGPSVSYACDHADCTSLGYGSSCGNLNLAQNVSYAFNSYYQVSNQLDS 429

Query: 432 ACKFPHLSVITTQDPSVGSCKFVVMTQPSEVA-VNEKSKALRHSAESLLFFLCLFIL 482
           ACKFP LS+++T+DPSVGSCKF +M +  + +  +      R +A  LL  +CL+I+
Sbjct: 430 ACKFPGLSIVSTRDPSVGSCKFKIMIKSEDASEASAMMPITRSTAVLLLLSICLYIV 483

BLAST of IVF0024251 vs. TAIR 10
Match: AT5G58090.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 605.9 bits (1561), Expect = 2.8e-173
Identity = 284/453 (62.69%), Postives = 358/453 (79.03%), Query Frame = 0

Query: 8   VSWRFFLVLLLSSAAVSSVIGIGVNWGTQATHPLPPGTVVKMLKDNGIQKVKLFDADPVV 67
           + W   L+LL  +        IG NWGTQA+HPLPP  VV+ML++NGIQKVKLFDA+   
Sbjct: 1   MGWGSVLLLLAVALLCQRASSIGANWGTQASHPLPPDIVVRMLRENGIQKVKLFDAEYDT 60

Query: 68  LKALGKSGIEVMVGIPNDLLYSLANSIQSAETGLLKTCLLIFHLAV------YVAVGNEP 127
           L+ALGKSGIEVMVGIPN++L +LA+S+++AE  + K   +  H++       YVAVGNEP
Sbjct: 61  LRALGKSGIEVMVGIPNEMLATLASSLKAAEKWVAKN--VSTHISTDNVNIRYVAVGNEP 120

Query: 128 FLKTFNGSFIGTTFPALQNVQAAIVKAGLSNRVKVTVPMNADVYQTSTGLPSGGDFRSDI 187
           FL T+NGS++ TTFPAL+N+Q AI+KAGL N+VKVT P+NADVY +ST  PSGGDFR++I
Sbjct: 121 FLSTYNGSYLSTTFPALRNIQIAIIKAGLQNQVKVTCPLNADVYDSSTTFPSGGDFRANI 180

Query: 188 RDLMLSIVKFLSDSASPFTVNIYPFISLYEDPSFPIDYAFFDGFSHALNDNGQIYENVFT 247
           RDLM++IVKFLS++  PFTVNIYP+ISLY +P FP+DYAFFDG +  LND G  Y N+F 
Sbjct: 181 RDLMITIVKFLSENGGPFTVNIYPYISLYTNPDFPVDYAFFDGNAQPLNDGGTFYYNMFD 240

Query: 248 ANYDTLVSSLQKNGFPNMSIIVGEIGWPTDGDRNANLQFAKRFSQGFISHFLSGQGTPMR 307
           ANYDTLV +L+KNGF NM II+GEIGWPTDGD NANL +AK+F+QGF++H   G+GTP R
Sbjct: 241 ANYDTLVHALEKNGFGNMPIIIGEIGWPTDGDSNANLDYAKKFNQGFMAHISGGKGTPRR 300

Query: 308 PGPIDVYLFSLIDEDAKSILPGNFERHWGLFYYDGRPKYMLQM-TNSTNGLVPASNVKYL 367
           PGPID YLFSLIDEDAKS+ PG FERHWG+F +DG PKY L + T +T  L+ A  V+YL
Sbjct: 301 PGPIDAYLFSLIDEDAKSVQPGYFERHWGIFTFDGLPKYALNLGTTNTGALIQAKGVRYL 360

Query: 368 AKKWCVLAPSASIDDPQIAPSVSYACENGDCTSLGLGTSCGTLDARQNISYAFNSYYQIK 427
            +KWCV+ P+  +DDPQ+AP+VSYAC  GDCTSLG+GTSC  LD +QNISYAFNSYYQI+
Sbjct: 361 ERKWCVMKPNVRLDDPQVAPAVSYACSLGDCTSLGVGTSCANLDGKQNISYAFNSYYQIQ 420

Query: 428 NQLASACKFPHLSVITTQDPSVGSCKFVVMTQP 454
           +QL +ACKFP++S +T  DPS G+C+F +M +P
Sbjct: 421 DQLDTACKFPNISEVTKTDPSTGTCRFPIMIEP 451

BLAST of IVF0024251 vs. TAIR 10
Match: AT5G20870.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 490.7 bits (1262), Expect = 1.3e-138
Identity = 250/450 (55.56%), Postives = 317/450 (70.44%), Query Frame = 0

Query: 12  FFLVLLLSSAAVSSVIGIGVNWGTQATHPLPPGTVVKMLKDNGIQKVKLFDADPVVLKAL 71
           F L +      + +V G+  NWGTQA+HPLPP  VVK+L+DNG  KVKLF+ADP  L+AL
Sbjct: 13  FCLSIFFLQNILKNVEGLACNWGTQASHPLPPNIVVKLLRDNGFNKVKLFEADPGALRAL 72

Query: 72  GKSGIEVMVGIPNDLLYSLANSIQSAETGLLKTCLLIFHL----AVYVAVGNEPFLKTFN 131
           GKSGI+VMVGIPNDLL ++A+++ +AE  + +              YVAVGNEPFLKT+N
Sbjct: 73  GKSGIQVMVGIPNDLLATMASTVTNAELWVQQNVSQYISRYGTDIRYVAVGNEPFLKTYN 132

Query: 132 GSFIGTTFPALQNVQAAIVKAGLSNRVKVTVPMNADVYQTSTGLPSGGDFRSDIRDLMLS 191
             F+ +T+PALQNVQAA+VKAGL  +VKVTVP+NADVY++S GLPS GDFRSDI+ LM+S
Sbjct: 133 NRFVRSTYPALQNVQAALVKAGLGRQVKVTVPLNADVYESSDGLPSSGDFRSDIKTLMIS 192

Query: 192 IVKFLSDSASPFTVNIYPFISLYEDPSFPIDYAFF-----DGFSHALNDNGQIYENVFTA 251
           IV+FL+DS SP T NIYPF+SL  DP+FP +YAFF      G +  + D    Y NVF A
Sbjct: 193 IVRFLADSVSPITFNIYPFLSLNADPNFPREYAFFPNGGGGGGAKPVVDGSISYTNVFDA 252

Query: 252 NYDTLVSSLQKNGFP--NMSIIVGEIGWPTDGDRNANLQFAKRFSQGFISHFLSGQGTPM 311
           N+DTLVS+L+KNGF    + IIVGE+GWPTDGD+NAN   A+RF+QG ++  L GQGTP 
Sbjct: 253 NFDTLVSALEKNGFDANKIEIIVGEVGWPTDGDQNANPAMAQRFNQGLLNRILQGQGTPR 312

Query: 312 RPGPIDVYLFSLIDEDAKSILPGNFERHWGLFYYDGRPKYMLQMTNSTNGLVPASNVKYL 371
           R    +VY+FSL+DEDAKSI PG FERHWG+F YDG  KY L + N    LVP   V+Y 
Sbjct: 313 RRMAPEVYIFSLVDEDAKSIDPGKFERHWGIFSYDGAVKYPLSLGNG-RPLVPTKGVRYQ 372

Query: 372 AKKWCVLAPSASIDDPQI-APSVSYACENGDCTSLGLGTSCGTLDARQNISYAFNSYYQI 431
           A++WCVL+  A+ +       S +YAC+N DCTSLG G+SC  LD   N SYAFN Y+Q 
Sbjct: 373 AREWCVLSTQAAGNGAATWQASATYACQNADCTSLGPGSSCAALDPTANASYAFNMYFQK 432

Query: 432 KNQLASACKFPHLSVITTQDPSVGSCKFVV 450
            +    +C F +L V+T  DPS GSC+F +
Sbjct: 433 MDHRRGSCDFNNLGVVTKIDPSSGSCRFPI 461

BLAST of IVF0024251 vs. TAIR 10
Match: AT3G04010.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 455.7 bits (1171), Expect = 4.7e-128
Identity = 228/444 (51.35%), Postives = 296/444 (66.67%), Query Frame = 0

Query: 13  FLVLLLSSAAVSSVIGIGVNWGTQATHPLPPGTVVKMLKDNGIQKVKLFDADPVVLKALG 72
           F+++         V G+GVNWGT A+H LPP TVV+MLKDN IQKVKLFDAD   + AL 
Sbjct: 17  FIIISAVCFLSGGVSGLGVNWGTMASHQLPPKTVVEMLKDNNIQKVKLFDADTNTMGALA 76

Query: 73  KSGIEVMVGIPNDLLYSLANSIQSAETGLLKTCLLIFHLAV---YVAVGNEPFLKTFNGS 132
            SG+EVMV IPNDLL ++ N  ++ +          F+  V   YVAVGNEPFL  +NGS
Sbjct: 77  GSGVEVMVAIPNDLLLAMGNYQRAKDWVQRNVSRFNFNNGVKIKYVAVGNEPFLTAYNGS 136

Query: 133 FIGTTFPALQNVQAAIVKAGLSNRVKVTVPMNADVYQT--STGLPSGGDFRSDIRDLMLS 192
           FI  T+PAL N+Q A+ +AG+ +  K TVP+NADVY +     +PS G FRSDI   M  
Sbjct: 137 FINLTYPALFNIQTALNEAGVGDFTKATVPLNADVYNSPPDNQVPSAGRFRSDIIQEMTQ 196

Query: 193 IVKFLSDSASPFTVNIYPFISLYEDPSFPIDYAFFDGFSHALNDNGQIYENVFTANYDTL 252
           IV FL+ + +PFTVNIYPF+SLY    FP +YAFFDG  + +NDNG IY NVF A++DTL
Sbjct: 197 IVNFLAQNKAPFTVNIYPFLSLYLSSDFPFEYAFFDG-QNTVNDNGVIYTNVFDASFDTL 256

Query: 253 VSSLQKNGFPNMSIIVGEIGWPTDGDRNANLQFAKRFSQGFISHFLSGQGTPMRPGPIDV 312
           ++SL      NM +IVGE+GWPTDGD+NAN+  A+RF  G +    +  GTPMR G I+V
Sbjct: 257 LASLNALNHGNMEVIVGEVGWPTDGDKNANVPNAERFYSGLLPRLANNVGTPMRKGYIEV 316

Query: 313 YLFSLIDEDAKSILPGNFERHWGLFYYDGRPKYMLQM--TNSTNGLVPASNVKYLAKKWC 372
           YLF  IDEDAKS+ PGNFERHWG+F +DG+PK+ +          L  A NV+Y   +WC
Sbjct: 317 YLFGFIDEDAKSVAPGNFERHWGIFKFDGQPKFPVDFRGQGQKKFLTGAQNVQYFLNQWC 376

Query: 373 VLAPSASIDDPQIAPSVSYACENGDCTSLGLGTSCGTLDARQNISYAFNSYYQIKNQLAS 432
           +  P+   +  ++  +++YAC + DCT+LG G+SCG LDA  N SYAFN Y+Q++NQ A 
Sbjct: 377 MFNPNGRGNMSRLGDNINYACSHSDCTALGYGSSCGNLDANGNASYAFNMYFQVQNQEAQ 436

Query: 433 ACKFPHLSVITTQDPSVGSCKFVV 450
           AC F  L+ ITTQ+ S G C F +
Sbjct: 437 ACDFEGLATITTQNISQGQCNFPI 459

BLAST of IVF0024251 vs. TAIR 10
Match: AT3G24330.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 451.8 bits (1161), Expect = 6.8e-127
Identity = 241/482 (50.00%), Postives = 315/482 (65.35%), Query Frame = 0

Query: 12  FFLVLLLSSAAVSSVIGIGVNWGTQATHPLPPGTVVKMLKDNGIQKVKLFDADPVVLKAL 71
           +FL++   S A S+   +GVNWG  A+H LPP  VVKML DN   K+KLF+AD  +L AL
Sbjct: 22  YFLIISQVSIASSNTSNVGVNWGIMASHQLPPEKVVKMLMDNSFTKLKLFEADQNILDAL 81

Query: 72  GKSGIEVMVGIPNDLLYSLANSIQSAETGLLKTCLLIFHLA----VYVAVGNEPFLKTFN 131
             S IEVM+GIPN  L  +A     A + + +      +       Y+AVGNEPFL+T+N
Sbjct: 82  IGSDIEVMIGIPNRFLKEMAQDTSVAASWVEENVTAYSYNGGVNIKYIAVGNEPFLQTYN 141

Query: 132 GSFIGTTFPALQNVQAAIVKAGLSNRVKVTVPMNADVY--QTSTGLPSGGDFRSDIRDLM 191
           G+++  T PAL N+Q A+ +A L N VKVTVP NAD+Y    +  +PS GDFR ++RD  
Sbjct: 142 GTYVEFTLPALINIQRALEEADLKN-VKVTVPFNADIYFSPEANPVPSAGDFRPELRDAT 201

Query: 192 LSIVKFLSDSASPFTVNIYPFISLYEDPSFPIDYAFFDGFSHALNDNGQIYENVFTANYD 251
           + I+ FL    SPFTVNIYPF+SLY +  FP+D+AFFDG + +L D   +Y NVF AN D
Sbjct: 202 IEIINFLYSHDSPFTVNIYPFLSLYGNAYFPLDFAFFDGTNKSLRDGNLVYTNVFDANLD 261

Query: 252 TLVSSLQKNGFPNMSIIVGEIGWPTDGDRNANLQFAKRFSQGFISHFLSGQGTPMRPGPI 311
           TL+ ++++  F  M IIVGE+GWPTDGD+NAN++ AKRF+QG + H +SG GTP R G I
Sbjct: 262 TLICAMERYSFLGMKIIVGEVGWPTDGDKNANVKSAKRFNQGMVKHAMSGNGTPARKGVI 321

Query: 312 -DVYLFSLIDEDAKSILPGNFERHWGLFYYDGRPKYMLQMTNSTNG--LVPASNVKYLAK 371
            DVYLFSL+DEDAKSI PG FERHWG+F +DGRPKY L ++   N   LVP  +VKYL K
Sbjct: 322 MDVYLFSLVDEDAKSIAPGTFERHWGIFEFDGRPKYELDLSGKGNDKPLVPVEDVKYLPK 381

Query: 372 KWCVLAPSA-SIDDPQIAPSVSYACENGDCTSLGLGTSCGTLDARQNISYAFNSYYQIKN 431
            WC+L P+A ++DD  +  ++ YAC   DCT+LG G+SC  L A  N+SYAFN YYQ+ +
Sbjct: 382 TWCILDPNAYNLDD--LPDNIDYACSLSDCTALGYGSSCNHLTATGNVSYAFNMYYQMHD 441

Query: 432 QLASACKFPHLSVITTQDPSVGSCKFVVM--TQPSEVAVNEKSKALRHSAESLLFFLCLF 482
           Q    C F  L +IT +DPS   C+F VM  T  S       S+ L   A ++L  L L 
Sbjct: 442 QKTWDCDFLGLGLITDEDPSDELCEFPVMIDTGDSTRLQPGSSRVLTRVAAAVLVMLVLP 500

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9M0881.3e-17862.47Glucan endo-1,3-beta-glucosidase 5 OS=Arabidopsis thaliana OX=3702 GN=At4g31140 ... [more]
Q93Z084.0e-17262.69Glucan endo-1,3-beta-glucosidase 6 OS=Arabidopsis thaliana OX=3702 GN=At5g58090 ... [more]
Q6NKW92.8e-12551.29Glucan endo-1,3-beta-glucosidase 8 OS=Arabidopsis thaliana OX=3702 GN=At1g64760 ... [more]
Q9FGH41.1e-9441.72Glucan endo-1,3-beta-glucosidase 9 OS=Arabidopsis thaliana OX=3702 GN=At5g58480 ... [more]
Q9FJU91.6e-5631.60Glucan endo-1,3-beta-glucosidase 13 OS=Arabidopsis thaliana OX=3702 GN=At5g56590... [more]
Match NameE-valueIdentityDescription
A0A5D3E7572.0e-26796.31(1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A1S3CR172.0e-26796.31(1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103503788 PE=3 SV... [more]
A0A0A0LGR29.9e-25992.21(1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_3G880540 PE=3... [more]
A0A6J1J1958.1e-23785.04(1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111480957 PE=... [more]
A0A6J1FPD11.1e-23685.04(1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111446059 P... [more]
Match NameE-valueIdentityDescription
XP_008466353.10.096.31PREDICTED: glucan endo-1,3-beta-glucosidase 5 [Cucumis melo] >KAA0038781.1 gluca... [more]
XP_004136331.10.092.21glucan endo-1,3-beta-glucosidase 5 [Cucumis sativus] >KGN60139.1 hypothetical pr... [more]
XP_038898735.14.72e-31489.69glucan endo-1,3-beta-glucosidase 5 [Benincasa hispida][more]
XP_023524183.13.33e-29885.25glucan endo-1,3-beta-glucosidase 5 [Cucurbita pepo subsp. pepo][more]
XP_022981965.19.53e-29885.04glucan endo-1,3-beta-glucosidase 5 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT4G31140.19.0e-18062.47O-Glycosyl hydrolases family 17 protein [more]
AT5G58090.12.8e-17362.69O-Glycosyl hydrolases family 17 protein [more]
AT5G20870.11.3e-13855.56O-Glycosyl hydrolases family 17 protein [more]
AT3G04010.14.7e-12851.35O-Glycosyl hydrolases family 17 protein [more]
AT3G24330.16.8e-12750.00O-Glycosyl hydrolases family 17 protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012946X8 domainSMARTSM00768X8_clscoord: 363..447
e-value: 2.4E-29
score: 113.5
IPR012946X8 domainPFAMPF07983X8coord: 363..430
e-value: 6.6E-15
score: 55.6
IPR000490Glycoside hydrolase family 17PFAMPF00332Glyco_hydro_17coord: 29..343
e-value: 1.5E-58
score: 198.9
IPR000490Glycoside hydrolase family 17PROSITEPS00587GLYCOSYL_HYDROL_F17coord: 259..272
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 28..350
e-value: 5.3E-103
score: 346.3
NoneNo IPR availableGENE3D1.20.58.1040coord: 359..461
e-value: 4.6E-7
score: 32.1
NoneNo IPR availablePANTHERPTHR32227:SF279GLUCAN ENDO-1,3-BETA-GLUCOSIDASE 5coord: 15..463
IPR044965Glycoside hydrolase family 17, plantPANTHERPTHR32227GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATEDcoord: 15..463
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 29..342

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
IVF0024251.2IVF0024251.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
cellular_component GO:0046658 anchored component of plasma membrane
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0042973 glucan endo-1,3-beta-D-glucosidase activity
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds