IVF0024075 (gene) Melon (IVF77) v1

BLAST of IVF0024075 vs. ExPASy Swiss-Prot
Match: O64495 (Subtilisin-like protease SBT1.2 OS=Arabidopsis thaliana OX=3702 GN=SBT1.2 PE=2 SV=1)

HSP 1 Score: 1011.1 bits (2613), Expect = 6.3e-294
Identity = 503/767 (65.58%), Postives = 611/767 (79.66%), Query Frame = 0

Query: 9   FLLFLYFLSLLVQPNTSTLQTYIIQLHPHGLITSVFDSKLQWHLSFLEQTL----SAEED 68
           FLLF    S ++Q      QTYI+QLHP+      F SK  WHLSFL++ +      EE+
Sbjct: 12  FLLFCSSSSEILQK-----QTYIVQLHPNSETAKTFASKFDWHLSFLQEAVLGVEEEEEE 71

Query: 69  SSSRLLYSYSNAMEGFAAQLTETELEYLKKLPDVVAVREDRKYQIQTTYSHKFLGL-SVG 128
            SSRLLYSY +A+EGFAAQLTE+E E L+  P+VVAVR D   Q+QTTYS+KFLGL   G
Sbjct: 72  PSSRLLYSYGSAIEGFAAQLTESEAEILRYSPEVVAVRPDHVLQVQTTYSYKFLGLDGFG 131

Query: 129 TQGVWQKSSMGQGAIVGLLDTGVWPESPSFSDSKMPPIPQKWRGACQEGQDFNSSNCNRK 188
             GVW KS  GQG I+G+LDTGVWPESPSF D+ MP IP+KW+G CQEG+ F+SS+CNRK
Sbjct: 132 NSGVWSKSRFGQGTIIGVLDTGVWPESPSFDDTGMPSIPRKWKGICQEGESFSSSSCNRK 191

Query: 189 LIGAKFFIKGHHVASSLPS--DVVQEYVSPRDSHGHGTHTSSTAAGASVADASVFGNGAG 248
           LIGA+FFI+GH VA+S     ++ +EY+S RDS GHGTHT+ST  G+SV+ A+V GNGAG
Sbjct: 192 LIGARFFIRGHRVANSPEESPNMPREYISARDSTGHGTHTASTVGGSSVSMANVLGNGAG 251

Query: 249 VAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAISDGVDILSLSLGGFPLPFFDDSIAI 308
           VA+GMAPGAHIAVYKVCWF+GCYSSDI+AA+D AI D VD+LSLSLGGFP+P +DD+IAI
Sbjct: 252 VARGMAPGAHIAVYKVCWFNGCYSSDILAAIDVAIQDKVDVLSLSLGGFPIPLYDDTIAI 311

Query: 309 G----------IVCAAGNNGPTQSSVANVAPWITTIGAGTLDRRFPAIVRLSNGEAIYGE 368
           G          ++CAAGNNGP +SSVAN APW++TIGAGTLDRRFPA+VRL+NG+ +YGE
Sbjct: 312 GTFRAMERGISVICAAGNNGPIESSVANTAPWVSTIGAGTLDRRFPAVVRLANGKLLYGE 371

Query: 369 SMYPGNKFKQATKELEVVYLTGGQMGGELCLKGSLPREKVQGKMVVCDRGVNGRSEKGQI 428
           S+YPG   K A +E+EV+Y+TGG  G E CL+GSLPRE+++GKMV+CDRGVNGRSEKG+ 
Sbjct: 372 SLYPGKGIKNAGREVEVIYVTGGDKGSEFCLRGSLPREEIRGKMVICDRGVNGRSEKGEA 431

Query: 429 VKESGGAAMILANSEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTNPKARIQFGGT 488
           VKE+GG AMILAN+EIN EED +DVH+LPATLIG+ E+  LKAY+N T  PKARI FGGT
Sbjct: 432 VKEAGGVAMILANTEINQEEDSIDVHLLPATLIGYTESVLLKAYVNATVKPKARIIFGGT 491

Query: 489 VIGRSRAPSVAQFSSRGPSLSNPSTLKPDVIAPGVNIIAAWPQNLGPTSLPEDSRRSNFT 548
           VIGRSRAP VAQFS+RGPSL+NPS LKPD+IAPGVNIIAAWPQNLGPT LP DSRR NFT
Sbjct: 492 VIGRSRAPEVAQFSARGPSLANPSILKPDMIAPGVNIIAAWPQNLGPTGLPYDSRRVNFT 551

Query: 549 VMSGTSMACPHVSGITALIHSAHPKWTPAAIKSAIMTTADVTDHFGKQILDGNKPADVFA 608
           VMSGTSM+CPHVSGITALI SA+P W+PAAIKSA+MTTAD+ D  GK I DGNKPA VFA
Sbjct: 552 VMSGTSMSCPHVSGITALIRSAYPNWSPAAIKSALMTTADLYDRQGKAIKDGNKPAGVFA 611

Query: 609 MGAGHVNPTKAIDPGLVYDIKPYEYIIHLCALGYTHSEIFIITHMNVSCHKVLQMNKGFT 668
           +GAGHVNP KAI+PGLVY+I+P +YI +LC LG+T S+I  ITH NVSC+ +L+ N GF+
Sbjct: 612 IGAGHVNPQKAINPGLVYNIQPVDYITYLCTLGFTRSDILAITHKNVSCNGILRKNPGFS 671

Query: 669 LNYPSMSVIFKHGTTSKMVSRRLTNVGSTNSIYEVKVTAPEGVRVRVKPRRLVFKHVNQS 728
           LNYPS++VIFK G T++M++RR+TNVGS NSIY V V APEG++V V P+RLVFKHV+Q+
Sbjct: 672 LNYPSIAVIFKRGKTTEMITRRVTNVGSPNSIYSVNVKAPEGIKVIVNPKRLVFKHVDQT 731

Query: 729 LNYKVWFI-----------EVYRRDLTWIHCENSKYKVRSPIVVTWK 748
           L+Y+VWF+              +  LTW++  N   +VRSPI VT K
Sbjct: 732 LSYRVWFVLKKKNRGGKVASFAQGQLTWVNSHNLMQRVRSPISVTLK 773

BLAST of IVF0024075 vs. ExPASy Swiss-Prot
Match: Q9ZUF6 (Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana OX=3702 GN=SBT1.8 PE=1 SV=1)

HSP 1 Score: 628.2 bits (1619), Expect = 1.2e-178
Identity = 350/752 (46.54%), Postives = 473/752 (62.90%), Query Frame = 0

Query: 15  FLSLLVQPNTSTLQTYIIQLHPHGLITSVFDSKLQWHLSFLEQTLSAEEDSSSRLLYSYS 74
           FL LL+  +T+  +TYII+++ H      F +   W+ S L        +S S LLY+Y+
Sbjct: 17  FLFLLL--HTTAKKTYIIRVN-HSDKPESFLTHHDWYTSQL--------NSESSLLYTYT 76

Query: 75  NAMEGFAAQLTETELE-YLKKLPDVVAVREDRKYQIQTTYSHKFLGLSVGTQGVWQKSSM 134
            +  GF+A L  TE +  L     ++ + ED  Y + TT + +FLGL+    GV    S 
Sbjct: 77  TSFHGFSAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLN-SEFGVHDLGSS 136

Query: 135 GQGAIVGLLDTGVWPESPSFSDSKMPPIPQKWRGACQEGQDFNSSNCNRKLIGAKFFIKG 194
             G I+G+LDTGVWPES SF D+ MP IP KW+G C+ G DF+S  CN+KLIGA+ F KG
Sbjct: 137 SNGVIIGVLDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARSFSKG 196

Query: 195 HHVASSLPSDVVQEYVSPRDSHGHGTHTSSTAAGASVADASVFGNGAGVAQGMAPGAHIA 254
             +AS       +E VSPRD  GHGTHTS+TAAG++V +AS  G  AG A+GMA  A +A
Sbjct: 197 FQMASGGGFSSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRARVA 256

Query: 255 VYKVCWFSGCYSSDIVAAMDSAISDGVDILSLSLGGFPLPFFDDSIAIG----------I 314
            YKVCW +GC+ SDI+AAMD AI DGVD+LSLSLGG   P++ D+IAIG          +
Sbjct: 257 TYKVCWSTGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFSAMERGVFV 316

Query: 315 VCAAGNNGPTQSSVANVAPWITTIGAGTLDRRFPAIVRLSNGEAIYGESMYPGNKFKQAT 374
            C+AGN+GPT++SVANVAPW+ T+GAGTLDR FPA   L NG+ + G S+Y G      T
Sbjct: 317 SCSAGNSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSG--VGMGT 376

Query: 375 KELEVVYLTGGQMGGELCLKGSLPREKVQGKMVVCDRGVNGRSEKGQIVKESGGAAMILA 434
           K LE+VY  G      LCL GSL    V+GK+VVCDRGVN R EKG +V+++GG  MI+A
Sbjct: 377 KPLELVYNKGNSSSSNLCLPGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDAGGLGMIMA 436

Query: 435 NSEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTNPKARIQFGGTVIGRSRAPSVAQ 494
           N+  + EE + D H+LPA  +G    + L+ Y+ + + P A + F GTV+    +P VA 
Sbjct: 437 NTAASGEELVADSHLLPAIAVGKKTGDLLREYVKSDSKPTALLVFKGTVLDVKPSPVVAA 496

Query: 495 FSSRGPSLSNPSTLKPDVIAPGVNIIAAWPQNLGPTSLPEDSRRSNFTVMSGTSMACPHV 554
           FSSRGP+   P  LKPDVI PGVNI+A W   +GPT L +DSRR+ F +MSGTSM+CPH+
Sbjct: 497 FSSRGPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMSCPHI 556

Query: 555 SGITALIHSAHPKWTPAAIKSAIMTTADVTDHFGKQILDG--NKPADVFAMGAGHVNPTK 614
           SG+  L+ +AHP+W+P+AIKSA+MTTA V D+    + D   N  ++ +A G+GHV+P K
Sbjct: 557 SGLAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAADNSLSNPYAHGSGHVDPQK 616

Query: 615 AIDPGLVYDIKPYEYIIHLCALGYTHSEIF-IITHMNVSCHKVLQMNKGFTLNYPSMSVI 674
           A+ PGLVYDI   EYI  LC+L YT   I  I+   +V+C K  + +    LNYPS SV+
Sbjct: 617 ALSPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVNCSK--KFSDPGQLNYPSFSVL 676

Query: 675 FKHGTTSKMVSRRLTNVGSTNSIYEVKVTAPEGVRVRVKPRRLVFKHVNQSLNYKVWFIE 734
           F  G      +R +TNVG+ +S+Y+V V     V + VKP +L FK V +   Y V F+ 
Sbjct: 677 F-GGKRVVRYTREVTNVGAASSVYKVTVNGAPSVGISVKPSKLSFKSVGEKKRYTVTFVS 736

Query: 735 ------VYRRDLTWIHCENSKYKVRSPIVVTW 747
                   + +   I   N +++VRSP+  +W
Sbjct: 737 KKGVSMTNKAEFGSITWSNPQHEVRSPVAFSW 751

BLAST of IVF0024075 vs. ExPASy Swiss-Prot
Match: Q9LUM3 (Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana OX=3702 GN=SBT1.5 PE=2 SV=1)

HSP 1 Score: 609.8 bits (1571), Expect = 4.2e-173
Identity = 338/781 (43.28%), Postives = 477/781 (61.08%), Query Frame = 0

Query: 7   MGFLLFLYFLSLLVQPNTSTLQ----TYIIQLHPHGLITSVFDSKLQWHLSFLEQTLSAE 66
           M F  + +FL  L  P++S       TYI+ +  H    S+F +   W+ S    +L++ 
Sbjct: 1   MAFFFYFFFLLTLSSPSSSASSSNSLTYIVHV-DHEAKPSIFPTHFHWYTS----SLASL 60

Query: 67  EDSSSRLLYSYSNAMEGFAAQLTETELEYLKKLPDVVAVREDRKYQIQTTYSHKFLGL-S 126
             S   ++++Y     GF+A+LT  +   L   P V++V  ++   + TT S +FLGL S
Sbjct: 61  TSSPPSIIHTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRS 120

Query: 127 VGTQGVWQKSSMGQGAIVGLLDTGVWPESPSFSDSKMPPIPQKWRGACQEGQDFNSSNCN 186
               G+ ++S  G   ++G++DTGVWPE PSF D  + P+P KW+G C   QDF  S CN
Sbjct: 121 TDKAGLLEESDFGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACN 180

Query: 187 RKLIGAKFFIKGHHVASSLPSDVVQEYVSPRDSHGHGTHTSSTAAGASVADASVFGNGAG 246
           RKL+GA+FF  G+   +   ++   E+ SPRDS GHGTHT+S +AG  V  AS  G   G
Sbjct: 181 RKLVGARFFCGGYEATNGKMNETT-EFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHG 240

Query: 247 VAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAISDGVDILSLSLGGFPLPFFDDSIAI 306
           VA GMAP A +A YKVCW SGCY SDI+AA D+A++DGVD++SLS+GG  +P++ D+IAI
Sbjct: 241 VAAGMAPKARLAAYKVCWNSGCYDSDILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAI 300

Query: 307 G----------IVCAAGNNGPTQSSVANVAPWITTIGAGTLDRRFPAIVRLSNGEAIYGE 366
           G          +  +AGN GP   +V NVAPW+TT+GAGT+DR FPA V+L NG+ I G 
Sbjct: 301 GAFGAIDRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGV 360

Query: 367 SMYPGNKFKQATKELEVVYLTGGQMGGE-----LCLKGSLPREKVQGKMVVCDRGVNGRS 426
           S+Y G       +   +VY  G  +GG+     LCL+GSL    V+GK+V+CDRG+N R+
Sbjct: 361 SVYGGPGLDPG-RMYPLVY-GGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRA 420

Query: 427 EKGQIVKESGGAAMILANSEINLEEDLVDVHVLPATLIGFAEANRLKAYIN------TTT 486
            KG+IV+++GG  MI+AN   + E  + D HVLPAT +G +  + ++ YI+      ++ 
Sbjct: 421 TKGEIVRKNGGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSK 480

Query: 487 NPKARIQFGGTVIGRSRAPSVAQFSSRGPSLSNPSTLKPDVIAPGVNIIAAWPQNLGPTS 546
           +P A I F GT +G   AP VA FS+RGP+   P  LKPDVIAPG+NI+AAWP  +GP+ 
Sbjct: 481 HPTATIVFKGTRLGIRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSG 540

Query: 547 LPEDSRRSNFTVMSGTSMACPHVSGITALIHSAHPKWTPAAIKSAIMTTADVTDHFGKQI 606
           +  D+RR+ F ++SGTSMACPHVSG+ AL+ +AHP W+PAAI+SA++TTA   D+ G+ +
Sbjct: 541 VTSDNRRTEFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPM 600

Query: 607 LDGN--KPADVFAMGAGHVNPTKAIDPGLVYDIKPYEYIIHLCALGYTHSEIFIITHMNV 666
           +D +    + V   G+GHV+PTKA+DPGLVYDI  Y+YI  LC   YT + I  IT    
Sbjct: 601 MDESTGNTSSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQA 660

Query: 667 SCHKVLQMNKGFTLNYPSMSVIFKHGTTSKMVS---RRLTNVGSTNSIYEVKVTAPEGVR 726
            C    +      LNYPS SV+F+    SKM +   R +TNVG ++S+YE+K+  P G  
Sbjct: 661 DCDGARRAGHVGNLNYPSFSVVFQQYGESKMSTHFIRTVTNVGDSDSVYEIKIRPPRGTT 720

Query: 727 VRVKPRRLVFKHVNQSLNYKV-----------WFIEVYRRDLTWIHCENSKYKVRSPIVV 746
           V V+P +L F+ V Q L++ V               V    + W    + K  V SP+VV
Sbjct: 721 VTVEPEKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVETGHIVW---SDGKRNVTSPLVV 770

BLAST of IVF0024075 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 604.7 bits (1558), Expect = 1.4e-171
Identity = 340/764 (44.50%), Postives = 467/764 (61.13%), Query Frame = 0

Query: 3   FNTRMGFLLFLYFLSLLVQPNTSTLQTYIIQLHPHGLITSVFDSKLQWHLSFLEQTLSAE 62
           F +   F L L      V  ++S   TYI+ +     + S FD    W+ S L       
Sbjct: 5   FLSSTAFFLLLCLGFCHVSSSSSDQGTYIVHM-AKSQMPSSFDLHSNWYDSSLRSI---- 64

Query: 63  EDSSSRLLYSYSNAMEGFAAQLTETELEYLKKLPDVVAVREDRKYQIQTTYSHKFLGLSV 122
              S+ LLY+Y NA+ GF+ +LT+ E + L   P V++V  + +Y++ TT +  FLGL  
Sbjct: 65  -SDSAELLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDE 124

Query: 123 GTQGVWQKSSMGQGAIVGLLDTGVWPESPSFSDSKMPPIPQKWRGACQEGQDFNSSNCNR 182
            T  ++ ++      +VG+LDTGVWPES S+SD    PIP  W+G C+ G +F +S CNR
Sbjct: 125 HTADLFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNR 184

Query: 183 KLIGAKFFIKGHHVASSLPSDVVQEYVSPRDSHGHGTHTSSTAAGASVADASVFGNGAGV 242
           KLIGA+FF +G+  ++  P D  +E  SPRD  GHGTHTSSTAAG+ V  AS+ G  +G 
Sbjct: 185 KLIGARFFARGYE-STMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGT 244

Query: 243 AQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAISDGVDILSLSLGGFPLPFFDDSIAIG 302
           A+GMAP A +AVYKVCW  GC+SSDI+AA+D AI+D V++LS+SLGG    ++ D +AIG
Sbjct: 245 ARGMAPRARVAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIG 304

Query: 303 ----------IVCAAGNNGPTQSSVANVAPWITTIGAGTLDRRFPAIVRLSNGEAIYGES 362
                     + C+AGN GP+ SS++NVAPWITT+GAGTLDR FPA+  L NG+   G S
Sbjct: 305 AFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVS 364

Query: 363 MYPGNKFKQATKELEVVYL--TGGQMGGELCLKGSLPREKVQGKMVVCDRGVNGRSEKGQ 422
           ++ G       K L  +Y         G LC+ G+L  EKV+GK+V+CDRG+N R +KG 
Sbjct: 365 LFKGEALPD--KLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGD 424

Query: 423 IVKESGGAAMILANSEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTNPKARIQFGG 482
           +VK +GG  MILAN+  N EE + D H+LPAT +G    + ++ Y+ T  NP A I   G
Sbjct: 425 VVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILG 484

Query: 483 TVIGRSRAPSVAQFSSRGPSLSNPSTLKPDVIAPGVNIIAAWPQNLGPTSLPEDSRRSNF 542
           TV+G   +P VA FSSRGP+   P+ LKPD+IAPGVNI+AAW    GPT L  DSRR  F
Sbjct: 485 TVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEF 544

Query: 543 TVMSGTSMACPHVSGITALIHSAHPKWTPAAIKSAIMTTADVTDHFGKQILD--GNKPAD 602
            ++SGTSM+CPHVSG+ AL+ S HP+W+PAAI+SA+MTTA  T   GK +LD    KP+ 
Sbjct: 545 NIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPST 604

Query: 603 VFAMGAGHVNPTKAIDPGLVYDIKPYEYIIHLCALGYTHSEIFIITHMNVSCHKVLQMNK 662
            F  GAGHV+PT A +PGL+YD+   +Y+  LCAL YT  +I  ++  N +C      + 
Sbjct: 605 PFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSYSV 664

Query: 663 GFTLNYPSMSVIFKHGTTSKMVSRRLTNVGSTNSIYEVKVTA-PEGVRVRVKPRRLVFKH 722
              LNYPS +V    G  +   +R +T+VG   + Y VKVT+   GV++ V+P  L FK 
Sbjct: 665 A-DLNYPSFAVNV-DGVGAYKYTRTVTSVGGAGT-YSVKVTSETTGVKISVEPAVLNFKE 724

Query: 723 VNQSLNYKVWFIEVYRR-----DLTWIHCENSKYKVRSPIVVTW 747
            N+  +Y V F     +         I   + K+ V SP+ ++W
Sbjct: 725 ANEKKSYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAISW 756

BLAST of IVF0024075 vs. ExPASy Swiss-Prot
Match: Q9FLI4 (Subtilisin-like protease SBT1.3 OS=Arabidopsis thaliana OX=3702 GN=SBT1.3 PE=2 SV=1)

HSP 1 Score: 598.2 bits (1541), Expect = 1.3e-169
Identity = 329/760 (43.29%), Postives = 462/760 (60.79%), Query Frame = 0

Query: 13  LYFLSLLVQPNTSTLQTYIIQLHPHGLITSVFDSKLQWHLSFL----EQTLSAEEDSSSR 72
           L FL        ST +TY+I +    +    + + LQW+ S +    +     EE +++R
Sbjct: 20  LIFLQAETTTQISTKKTYVIHMDKSAM-PLPYTNHLQWYSSKINSVTQHKSQEEEGNNNR 79

Query: 73  LLYSYSNAMEGFAAQLTETELEYLKKLPDVVAVREDRKYQIQTTYSHKFLGLS-VGTQGV 132
           +LY+Y  A  G AAQLT+ E E L++   VVAV  + +Y++ TT S  FLGL    ++ V
Sbjct: 80  ILYTYQTAFHGLAAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSPTFLGLERQESERV 139

Query: 133 WQKSSMGQGAIVGLLDTGVWPESPSFSDSKMPPIPQKWRGACQEGQDFNSSNCNRKLIGA 192
           W +       +VG+LDTG+WPES SF+D+ M P+P  WRGAC+ G+ F   NCNRK++GA
Sbjct: 140 WAERVTDHDVVVGVLDTGIWPESESFNDTGMSPVPATWRGACETGKRFLKRNCNRKIVGA 199

Query: 193 KFFIKGHHVASSLPSDVVQEYVSPRDSHGHGTHTSSTAAGASVADASVFGNGAGVAQGMA 252
           + F +G+  A+    D   EY SPRD  GHGTHT++T AG+ V  A++FG   G A+GMA
Sbjct: 200 RVFYRGYEAATG-KIDEELEYKSPRDRDGHGTHTAATVAGSPVKGANLFGFAYGTARGMA 259

Query: 253 PGAHIAVYKVCWFSGCYSSDIVAAMDSAISDGVDILSLSLGGFPLPFFDDSIAIG----- 312
             A +A YKVCW  GC+SSDI++A+D A++DGV +LS+SLGG    +  DS++I      
Sbjct: 260 QKARVAAYKVCWVGGCFSSDILSAVDQAVADGVQVLSISLGGGVSTYSRDSLSIATFGAM 319

Query: 313 -----IVCAAGNNGPTQSSVANVAPWITTIGAGTLDRRFPAIVRLSNGEAIYGESMYPGN 372
                + C+AGN GP   S+ NV+PWITT+GA T+DR FPA V++       G S+Y G 
Sbjct: 320 EMGVFVSCSAGNGGPDPISLTNVSPWITTVGASTMDRDFPATVKIGTMRTFKGVSLYKGR 379

Query: 373 KFKQATKELEVVYLTGGQMGGE---LCLKGSLPREKVQGKMVVCDRGVNGRSEKGQIVKE 432
                 K+  +VYL       +    CL G+L R  V GK+V+CDRGV  R +KGQ+VK 
Sbjct: 380 TVLPKNKQYPLVYLGRNASSPDPTSFCLDGALDRRHVAGKIVICDRGVTPRVQKGQVVKR 439

Query: 433 SGGAAMILANSEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTNPKARIQFGGTVIG 492
           +GG  M+L N+  N EE + D H+LPA  +G  E   +K Y  T+    A ++  GT IG
Sbjct: 440 AGGIGMVLTNTATNGEELVADSHMLPAVAVGEKEGKLIKQYAMTSKKATASLEILGTRIG 499

Query: 493 RSRAPSVAQFSSRGPSLSNPSTLKPDVIAPGVNIIAAWPQNLGPTSLPEDSRRSNFTVMS 552
              +P VA FSSRGP+  +   LKPD++APGVNI+AAW  ++ P+SL  D RR  F ++S
Sbjct: 500 IKPSPVVAAFSSRGPNFLSLEILKPDLLAPGVNILAAWTGDMAPSSLSSDPRRVKFNILS 559

Query: 553 GTSMACPHVSGITALIHSAHPKWTPAAIKSAIMTTADVTDHFGKQILD--GNKPADVFAM 612
           GTSM+CPHVSG+ ALI S HP W+PAAIKSA+MTTA V D+  K + D  G  P+  +  
Sbjct: 560 GTSMSCPHVSGVAALIKSRHPDWSPAAIKSALMTTAYVHDNMFKPLTDASGAAPSSPYDH 619

Query: 613 GAGHVNPTKAIDPGLVYDIKPYEYIIHLCALGYTHSEIFIIT-HMNVSCHKVLQMNKGFT 672
           GAGH++P +A DPGLVYDI P EY   LC    + S++ + T H N +C   L  N G  
Sbjct: 620 GAGHIDPLRATDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFTKHSNRTCKHTLAKNPG-N 679

Query: 673 LNYPSMSVIFKHGTTSKMVS--RRLTNVGSTNSIYEVKVTAPEGVRVRVKPRRLVFKHVN 732
           LNYP++S +F   T  K ++  R +TNVG   S Y+V V+  +G  V V+P+ L F   +
Sbjct: 680 LNYPAISALFPENTHVKAMTLRRTVTNVGPHISSYKVSVSPFKGASVTVQPKTLNFTSKH 739

Query: 733 QSLNYKVWF---IEVYRRDLTWIHCENSKYKVRSPIVVTW 747
           Q L+Y V F     + R +   +  +++ +KVRSP+++TW
Sbjct: 740 QKLSYTVTFRTRFRMKRPEFGGLVWKSTTHKVRSPVIITW 776

BLAST of IVF0024075 vs. ExPASy TrEMBL
Match: A0A5A7T534 (Subtilisin-like protease SBT1.2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold154G00500 PE=3 SV=1)

HSP 1 Score: 1468.8 bits (3801), Expect = 0.0e+00
Identity = 741/768 (96.48%), Postives = 743/768 (96.74%), Query Frame = 0

Query: 1   MDFNTRMGFLLFLYFLSLLVQPNTSTLQTYIIQLHPHGLITSVFDSKLQWHLSFLEQTLS 60
           MDFNTRMGFLLFLYFLSLLVQPNTSTLQTYIIQLHPHGLITSVFDSKLQWHLSFLEQTLS
Sbjct: 1   MDFNTRMGFLLFLYFLSLLVQPNTSTLQTYIIQLHPHGLITSVFDSKLQWHLSFLEQTLS 60

Query: 61  AEEDSSSRLLYSYSNAMEGFAAQLTETELEYLKKLPDVVAVREDRKYQIQTTYSHKFLGL 120
           AEEDSSSRLLYSYSNAMEGFAAQLTETELEYLKKLPDVVAVREDRKYQIQTTYSHKFLGL
Sbjct: 61  AEEDSSSRLLYSYSNAMEGFAAQLTETELEYLKKLPDVVAVREDRKYQIQTTYSHKFLGL 120

Query: 121 SVGTQGVWQKSSMGQGAIVGLLDTGVWPESPSFSDSKMPPIPQKWRGACQEGQDFNSSNC 180
           SVGTQGVWQKSSMGQGAIVGLLDTGVWPESPSFSDSKMPPIPQKWRGACQEGQDFNSSNC
Sbjct: 121 SVGTQGVWQKSSMGQGAIVGLLDTGVWPESPSFSDSKMPPIPQKWRGACQEGQDFNSSNC 180

Query: 181 NRKLIGAKFFIKGHHVASSLPSDVVQEYVSPRDSHGHGTHTSSTAAGASVADASVFGNGA 240
           NRKLIGAKFFIKGHHVASSLPSDVVQEYVSPRDSHGHGTHTSSTAAGASVADASVFGNGA
Sbjct: 181 NRKLIGAKFFIKGHHVASSLPSDVVQEYVSPRDSHGHGTHTSSTAAGASVADASVFGNGA 240

Query: 241 GVAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAISDGVDILSLSLGGFPLPFFDDSIA 300
           GVAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAISDGVDILSLSLGGFPLPFFDDSIA
Sbjct: 241 GVAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAISDGVDILSLSLGGFPLPFFDDSIA 300

Query: 301 IG----------IVCAAGNNGPTQSSVANVAPWITTIGAGTLDRRFPAIVRLSNGEAIYG 360
           IG          +VCAAGNNGPTQSSVANVAPWITTIGAGTLDRRFPAIVRLSNGEAIYG
Sbjct: 301 IGSFRAMQHGISVVCAAGNNGPTQSSVANVAPWITTIGAGTLDRRFPAIVRLSNGEAIYG 360

Query: 361 ESMYPGNKFKQATKELEVVYLTGGQMGGELCLKGSLPREKVQGKMVVCDRGVNGRSEKGQ 420
           ESMYPGNKFKQATKELEVVYLTGGQMGGELCLKGSLPREKVQGKMVVCDRGVNGRSEKGQ
Sbjct: 361 ESMYPGNKFKQATKELEVVYLTGGQMGGELCLKGSLPREKVQGKMVVCDRGVNGRSEKGQ 420

Query: 421 IVKESGGAAMILANSEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTNPKARIQFGG 480
           IVKESGGAAMILANSEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTNPKARIQFGG
Sbjct: 421 IVKESGGAAMILANSEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTNPKARIQFGG 480

Query: 481 TVIGRSRAPSVAQFSSRGPSLSNPSTLKPDVIAPGVNIIAAWPQNLGPTSLPEDSRRSNF 540
           TVIGRSRAPSVAQFSSRGPSLSNPSTLKPDVIAPGVNIIAAWPQNLGPTSLPEDSRRSNF
Sbjct: 481 TVIGRSRAPSVAQFSSRGPSLSNPSTLKPDVIAPGVNIIAAWPQNLGPTSLPEDSRRSNF 540

Query: 541 TVMSGTSMACPHVSGITALIHSAHPKWTPAAIKSAIMTTADVTDHFGKQILDGNKPADVF 600
           TVMSGTSMACPHVSGITALIHSAHPKWTPAAIKSAIMTTADVTDHFGKQILDGNKPADVF
Sbjct: 541 TVMSGTSMACPHVSGITALIHSAHPKWTPAAIKSAIMTTADVTDHFGKQILDGNKPADVF 600

Query: 601 AMGAGHVNPTKAIDPGLVYDIKPYEYIIHLCALGYTHSEIFIITHMNVSCHKVLQMNKGF 660
           AMGAGHVNPTKAIDPGLVYDIKPYEYIIHLCALGYTHSEIFIITHMNVSCHKVLQMNKGF
Sbjct: 601 AMGAGHVNPTKAIDPGLVYDIKPYEYIIHLCALGYTHSEIFIITHMNVSCHKVLQMNKGF 660

Query: 661 TLNYPSMSVIFKHGTTSKMVSRRLTNVGSTNSIYEVKVTAPEGVRVRVKPRRLVFKHVNQ 720
           TLNYPSMSVIFKHGTTSKMVSRRLTNVGSTNSIYEVKVTAPEGVRVRVKPRRLVFKHVNQ
Sbjct: 661 TLNYPSMSVIFKHGTTSKMVSRRLTNVGSTNSIYEVKVTAPEGVRVRVKPRRLVFKHVNQ 720

Query: 721 SLNYKVWF----------IEVYRRDLTWIHCENSKYKVRSPIVVTWKN 749
           SLNYKVWF          +     DLTWIHCENSKYKVRSPIVVTWKN
Sbjct: 721 SLNYKVWFMSEKGKEGRKVRFTEGDLTWIHCENSKYKVRSPIVVTWKN 768

BLAST of IVF0024075 vs. ExPASy TrEMBL
Match: A0A1S3BHA2 (subtilisin-like protease SBT1.2 OS=Cucumis melo OX=3656 GN=LOC103489836 PE=3 SV=1)

HSP 1 Score: 1468.8 bits (3801), Expect = 0.0e+00
Identity = 741/768 (96.48%), Postives = 743/768 (96.74%), Query Frame = 0

Query: 1   MDFNTRMGFLLFLYFLSLLVQPNTSTLQTYIIQLHPHGLITSVFDSKLQWHLSFLEQTLS 60
           MDFNTRMGFLLFLYFLSLLVQPNTSTLQTYIIQLHPHGLITSVFDSKLQWHLSFLEQTLS
Sbjct: 1   MDFNTRMGFLLFLYFLSLLVQPNTSTLQTYIIQLHPHGLITSVFDSKLQWHLSFLEQTLS 60

Query: 61  AEEDSSSRLLYSYSNAMEGFAAQLTETELEYLKKLPDVVAVREDRKYQIQTTYSHKFLGL 120
           AEEDSSSRLLYSYSNAMEGFAAQLTETELEYLKKLPDVVAVREDRKYQIQTTYSHKFLGL
Sbjct: 61  AEEDSSSRLLYSYSNAMEGFAAQLTETELEYLKKLPDVVAVREDRKYQIQTTYSHKFLGL 120

Query: 121 SVGTQGVWQKSSMGQGAIVGLLDTGVWPESPSFSDSKMPPIPQKWRGACQEGQDFNSSNC 180
           SVGTQGVWQKSSMGQGAIVGLLDTGVWPESPSFSDSKMPPIPQKWRGACQEGQDFNSSNC
Sbjct: 121 SVGTQGVWQKSSMGQGAIVGLLDTGVWPESPSFSDSKMPPIPQKWRGACQEGQDFNSSNC 180

Query: 181 NRKLIGAKFFIKGHHVASSLPSDVVQEYVSPRDSHGHGTHTSSTAAGASVADASVFGNGA 240
           NRKLIGAKFFIKGHHVASSLPSDVVQEYVSPRDSHGHGTHTSSTAAGASVADASVFGNGA
Sbjct: 181 NRKLIGAKFFIKGHHVASSLPSDVVQEYVSPRDSHGHGTHTSSTAAGASVADASVFGNGA 240

Query: 241 GVAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAISDGVDILSLSLGGFPLPFFDDSIA 300
           GVAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAISDGVDILSLSLGGFPLPFFDDSIA
Sbjct: 241 GVAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAISDGVDILSLSLGGFPLPFFDDSIA 300

Query: 301 IG----------IVCAAGNNGPTQSSVANVAPWITTIGAGTLDRRFPAIVRLSNGEAIYG 360
           IG          +VCAAGNNGPTQSSVANVAPWITTIGAGTLDRRFPAIVRLSNGEAIYG
Sbjct: 301 IGSFRAMQHGISVVCAAGNNGPTQSSVANVAPWITTIGAGTLDRRFPAIVRLSNGEAIYG 360

Query: 361 ESMYPGNKFKQATKELEVVYLTGGQMGGELCLKGSLPREKVQGKMVVCDRGVNGRSEKGQ 420
           ESMYPGNKFKQATKELEVVYLTGGQMGGELCLKGSLPREKVQGKMVVCDRGVNGRSEKGQ
Sbjct: 361 ESMYPGNKFKQATKELEVVYLTGGQMGGELCLKGSLPREKVQGKMVVCDRGVNGRSEKGQ 420

Query: 421 IVKESGGAAMILANSEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTNPKARIQFGG 480
           IVKESGGAAMILANSEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTNPKARIQFGG
Sbjct: 421 IVKESGGAAMILANSEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTNPKARIQFGG 480

Query: 481 TVIGRSRAPSVAQFSSRGPSLSNPSTLKPDVIAPGVNIIAAWPQNLGPTSLPEDSRRSNF 540
           TVIGRSRAPSVAQFSSRGPSLSNPSTLKPDVIAPGVNIIAAWPQNLGPTSLPEDSRRSNF
Sbjct: 481 TVIGRSRAPSVAQFSSRGPSLSNPSTLKPDVIAPGVNIIAAWPQNLGPTSLPEDSRRSNF 540

Query: 541 TVMSGTSMACPHVSGITALIHSAHPKWTPAAIKSAIMTTADVTDHFGKQILDGNKPADVF 600
           TVMSGTSMACPHVSGITALIHSAHPKWTPAAIKSAIMTTADVTDHFGKQILDGNKPADVF
Sbjct: 541 TVMSGTSMACPHVSGITALIHSAHPKWTPAAIKSAIMTTADVTDHFGKQILDGNKPADVF 600

Query: 601 AMGAGHVNPTKAIDPGLVYDIKPYEYIIHLCALGYTHSEIFIITHMNVSCHKVLQMNKGF 660
           AMGAGHVNPTKAIDPGLVYDIKPYEYIIHLCALGYTHSEIFIITHMNVSCHKVLQMNKGF
Sbjct: 601 AMGAGHVNPTKAIDPGLVYDIKPYEYIIHLCALGYTHSEIFIITHMNVSCHKVLQMNKGF 660

Query: 661 TLNYPSMSVIFKHGTTSKMVSRRLTNVGSTNSIYEVKVTAPEGVRVRVKPRRLVFKHVNQ 720
           TLNYPSMSVIFKHGTTSKMVSRRLTNVGSTNSIYEVKVTAPEGVRVRVKPRRLVFKHVNQ
Sbjct: 661 TLNYPSMSVIFKHGTTSKMVSRRLTNVGSTNSIYEVKVTAPEGVRVRVKPRRLVFKHVNQ 720

Query: 721 SLNYKVWF----------IEVYRRDLTWIHCENSKYKVRSPIVVTWKN 749
           SLNYKVWF          +     DLTWIHCENSKYKVRSPIVVTWKN
Sbjct: 721 SLNYKVWFMSEKGKEGRKVRFTEGDLTWIHCENSKYKVRSPIVVTWKN 768

BLAST of IVF0024075 vs. ExPASy TrEMBL
Match: A0A0A0LDY7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G610820 PE=3 SV=1)

HSP 1 Score: 1439.9 bits (3726), Expect = 0.0e+00
Identity = 724/768 (94.27%), Postives = 736/768 (95.83%), Query Frame = 0

Query: 1   MDFNTRMGFLLFLYFLSLLVQPNTSTLQTYIIQLHPHGLITSVFDSKLQWHLSFLEQTLS 60
           MDFN RMGFLLFL FLSLLVQPNTSTLQTYIIQLHPHGLITSVFDSKLQWHLSFLEQ+LS
Sbjct: 1   MDFNARMGFLLFLCFLSLLVQPNTSTLQTYIIQLHPHGLITSVFDSKLQWHLSFLEQSLS 60

Query: 61  AEEDSSSRLLYSYSNAMEGFAAQLTETELEYLKKLPDVVAVREDRKYQIQTTYSHKFLGL 120
           AEEDSSSRLLYSYSNAMEGFAAQL+ETELEYLK+LPDVVAVREDRKYQIQTTYSHKFLGL
Sbjct: 61  AEEDSSSRLLYSYSNAMEGFAAQLSETELEYLKRLPDVVAVREDRKYQIQTTYSHKFLGL 120

Query: 121 SVGTQGVWQKSSMGQGAIVGLLDTGVWPESPSFSDSKMPPIPQKWRGACQEGQDFNSSNC 180
           SVGTQG+ QKSSMGQGAIVG+LDTGVWPESPSFSDSKMPP+PQKWRGACQEGQDFNSSNC
Sbjct: 121 SVGTQGLRQKSSMGQGAIVGVLDTGVWPESPSFSDSKMPPVPQKWRGACQEGQDFNSSNC 180

Query: 181 NRKLIGAKFFIKGHHVASSLPSDVVQEYVSPRDSHGHGTHTSSTAAGASVADASVFGNGA 240
           NRKLIGAKFFIKGHHVASSLPSDV QEYVSPRDSHGHGTHTSSTAAGASVADASVFGNGA
Sbjct: 181 NRKLIGAKFFIKGHHVASSLPSDVAQEYVSPRDSHGHGTHTSSTAAGASVADASVFGNGA 240

Query: 241 GVAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAISDGVDILSLSLGGFPLPFFDDSIA 300
           GVAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAI DGVDILSLSLGGFPLPFFDDSIA
Sbjct: 241 GVAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAIRDGVDILSLSLGGFPLPFFDDSIA 300

Query: 301 IG----------IVCAAGNNGPTQSSVANVAPWITTIGAGTLDRRFPAIVRLSNGEAIYG 360
           IG          +VCAAGNNGP QSSVANVAPWITTIGAGTLDRRFPAI+RLSNGEAIYG
Sbjct: 301 IGSFRAMQHGISVVCAAGNNGPIQSSVANVAPWITTIGAGTLDRRFPAIIRLSNGEAIYG 360

Query: 361 ESMYPGNKFKQATKELEVVYLTGGQMGGELCLKGSLPREKVQGKMVVCDRGVNGRSEKGQ 420
           ESMYPGNKFKQATKELEVVYLTGGQMGGELCLKGSLPREKVQGKMVVCDRGVNGRSEKGQ
Sbjct: 361 ESMYPGNKFKQATKELEVVYLTGGQMGGELCLKGSLPREKVQGKMVVCDRGVNGRSEKGQ 420

Query: 421 IVKESGGAAMILANSEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTNPKARIQFGG 480
           IVKESGGAAMILANSEINLEEDLVDVHVLPATLIGFAEANRLKAYINTT+NPKARIQFGG
Sbjct: 421 IVKESGGAAMILANSEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTSNPKARIQFGG 480

Query: 481 TVIGRSRAPSVAQFSSRGPSLSNPSTLKPDVIAPGVNIIAAWPQNLGPTSLPEDSRRSNF 540
           TVIGRSRAPSVAQFSSRGPSLSNPSTLKPDVIAPGVNIIAAWPQNLGPT LPEDSRRSNF
Sbjct: 481 TVIGRSRAPSVAQFSSRGPSLSNPSTLKPDVIAPGVNIIAAWPQNLGPTGLPEDSRRSNF 540

Query: 541 TVMSGTSMACPHVSGITALIHSAHPKWTPAAIKSAIMTTADVTDHFGKQILDGNKPADVF 600
           TVMSGTSMACPHVSGITALIHSAHPKWTPAAIKSAIMTTADVTDHFGKQILDGNKPADVF
Sbjct: 541 TVMSGTSMACPHVSGITALIHSAHPKWTPAAIKSAIMTTADVTDHFGKQILDGNKPADVF 600

Query: 601 AMGAGHVNPTKAIDPGLVYDIKPYEYIIHLCALGYTHSEIFIITHMNVSCHKVLQMNKGF 660
           AMGAGHVNPTKAIDPGLVYDIKPYEYIIHLCALGYTHSEIFIITHMNVSCHK+LQMNKGF
Sbjct: 601 AMGAGHVNPTKAIDPGLVYDIKPYEYIIHLCALGYTHSEIFIITHMNVSCHKILQMNKGF 660

Query: 661 TLNYPSMSVIFKHGTTSKMVSRRLTNVGSTNSIYEVKVTAPEGVRVRVKPRRLVFKHVNQ 720
           TLNYPS+SVIFKHGTTSKMVSRRLTNVGSTNSIYEVKVTAPEGVRVRVKPRRLVFKHVNQ
Sbjct: 661 TLNYPSISVIFKHGTTSKMVSRRLTNVGSTNSIYEVKVTAPEGVRVRVKPRRLVFKHVNQ 720

Query: 721 SLNYKVWF----------IEVYRRDLTWIHCENSKYKVRSPIVVTWKN 749
           SLNYKVWF          +     DLTWIHCENSKYKVRSPIVVTWKN
Sbjct: 721 SLNYKVWFMSEKGKEGRKVRFTEGDLTWIHCENSKYKVRSPIVVTWKN 768

BLAST of IVF0024075 vs. ExPASy TrEMBL
Match: A0A6J1HI32 (subtilisin-like protease SBT1.2 OS=Cucurbita moschata OX=3662 GN=LOC111463837 PE=3 SV=1)

HSP 1 Score: 1333.9 bits (3451), Expect = 0.0e+00
Identity = 674/769 (87.65%), Postives = 706/769 (91.81%), Query Frame = 0

Query: 1   MDFNTRMGF-LLFLYFLSLLVQPNTSTLQTYIIQLHPHGLITSVFDSKLQWHLSFLEQTL 60
           MDF ++M F  +FL FL L VQ N S LQTY+IQLHP GL +SVF+SKLQWHLSFLEQTL
Sbjct: 1   MDFYSQMVFSTIFLCFLLLQVQANASNLQTYMIQLHPQGLTSSVFESKLQWHLSFLEQTL 60

Query: 61  SAEEDSSSRLLYSYSNAMEGFAAQLTETELEYLKKLPDVVAVREDRKYQIQTTYSHKFLG 120
           S EED SSRLLYSYSNAMEGFAAQL+ETELEYLKKLPDVVAVR DRKY+IQTTYSHKFLG
Sbjct: 61  SVEEDCSSRLLYSYSNAMEGFAAQLSETELEYLKKLPDVVAVRADRKYEIQTTYSHKFLG 120

Query: 121 LSVGTQGVWQKSSMGQGAIVGLLDTGVWPESPSFSDSKMPPIPQKWRGACQEGQDFNSSN 180
           LSV TQGV  KSSMGQGAIVG+LDTGVWPESPSF DSKMPPIPQKWRG CQEGQDFNSS+
Sbjct: 121 LSVCTQGVCYKSSMGQGAIVGVLDTGVWPESPSFGDSKMPPIPQKWRGGCQEGQDFNSSS 180

Query: 181 CNRKLIGAKFFIKGHHVASSLPSDVVQEYVSPRDSHGHGTHTSSTAAGASVADASVFGNG 240
           CNRKLIGAKFFIKGHHVASS PSD VQEYVSPRDSHGHGTHTSSTAAGASVA ASVFGNG
Sbjct: 181 CNRKLIGAKFFIKGHHVASSPPSDTVQEYVSPRDSHGHGTHTSSTAAGASVAGASVFGNG 240

Query: 241 AGVAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAISDGVDILSLSLGGFPLPFFDDSI 300
           AGVAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAI DGVDILSLSLGGFPLPFFDDSI
Sbjct: 241 AGVAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAIRDGVDILSLSLGGFPLPFFDDSI 300

Query: 301 AIG----------IVCAAGNNGPTQSSVANVAPWITTIGAGTLDRRFPAIVRLSNGEAIY 360
           AIG          ++CAAGNNGPTQSSVANVAPWITTIGAGTLDR FPAIVRLSNGEAI 
Sbjct: 301 AIGSFRAMQHGISVICAAGNNGPTQSSVANVAPWITTIGAGTLDRTFPAIVRLSNGEAIP 360

Query: 361 GESMYPGNKFKQATKELEVVYLTGGQMGGELCLKGSLPREKVQGKMVVCDRGVNGRSEKG 420
           GESMYPGNKF QATKELE+VYLTGGQMG ELCLKGSLPRE+V+GKMVVCDRGVNGRSEKG
Sbjct: 361 GESMYPGNKFNQATKELELVYLTGGQMGSELCLKGSLPREQVEGKMVVCDRGVNGRSEKG 420

Query: 421 QIVKESGGAAMILANSEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTNPKARIQFG 480
           QIVKESGGAAMILAN+EINLEEDLVDVHVLPATLIGFAEANRLKAYINTTT+PKARIQFG
Sbjct: 421 QIVKESGGAAMILANTEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTDPKARIQFG 480

Query: 481 GTVIGRSRAPSVAQFSSRGPSLSNPSTLKPDVIAPGVNIIAAWPQNLGPTSLPEDSRRSN 540
           GTVIGR+RAPSVAQFSSRGPS +NPSTLKPDVIAPGVNIIAAWPQNL PT L EDSRRSN
Sbjct: 481 GTVIGRTRAPSVAQFSSRGPSPTNPSTLKPDVIAPGVNIIAAWPQNLAPTGLLEDSRRSN 540

Query: 541 FTVMSGTSMACPHVSGITALIHSAHPKWTPAAIKSAIMTTADVTDHFGKQILDGNKPADV 600
           FTVMSGTSM+CPHVSGI ALIHSAHPKWTPAAIKSAIMTTADVTD FGK ILDGNKPA V
Sbjct: 541 FTVMSGTSMSCPHVSGIAALIHSAHPKWTPAAIKSAIMTTADVTDRFGKPILDGNKPASV 600

Query: 601 FAMGAGHVNPTKAIDPGLVYDIKPYEYIIHLCALGYTHSEIFIITHMNVSCHKVLQMNKG 660
           FAMGAGHVNPT+AIDPGLVYDIKPY+Y+IHLC LGYTHSEIFIITHMNVSCHKVLQMNKG
Sbjct: 601 FAMGAGHVNPTRAIDPGLVYDIKPYDYVIHLCVLGYTHSEIFIITHMNVSCHKVLQMNKG 660

Query: 661 FTLNYPSMSVIFKHGTTSKMVSRRLTNVGSTNSIYEVKVTAPEGVRVRVKPRRLVFKHVN 720
           FTLNYPSMSV+FKHGTTSKMVSRRLTNVGS NS+YEV+VTAPEGV VRVKPRRLVFKHVN
Sbjct: 661 FTLNYPSMSVVFKHGTTSKMVSRRLTNVGSPNSVYEVEVTAPEGVSVRVKPRRLVFKHVN 720

Query: 721 QSLNYKVWFIEVYRRD----------LTWIHCENSKYKVRSPIVVTWKN 749
           +SL+YKVWF+   RR+          LTW+H ENSKY+VRSPIVVTWKN
Sbjct: 721 ESLSYKVWFMSEKRREGRKVSSTEGHLTWLHSENSKYRVRSPIVVTWKN 769

BLAST of IVF0024075 vs. ExPASy TrEMBL
Match: A0A6J1HUC6 (subtilisin-like protease SBT1.2 OS=Cucurbita maxima OX=3661 GN=LOC111466803 PE=3 SV=1)

HSP 1 Score: 1330.5 bits (3442), Expect = 0.0e+00
Identity = 672/769 (87.39%), Postives = 704/769 (91.55%), Query Frame = 0

Query: 1   MDFNTRMGF-LLFLYFLSLLVQPNTSTLQTYIIQLHPHGLITSVFDSKLQWHLSFLEQTL 60
           MDFN++M F  +FL FL L VQ N S LQTY+IQLHP GL +SVF+SKLQWHLSFLEQTL
Sbjct: 1   MDFNSQMVFSTIFLCFLWLQVQANASNLQTYMIQLHPQGLTSSVFESKLQWHLSFLEQTL 60

Query: 61  SAEEDSSSRLLYSYSNAMEGFAAQLTETELEYLKKLPDVVAVREDRKYQIQTTYSHKFLG 120
           S EED SSRLLYSYSNAMEGFAAQL+ETELEYLKKLPDVVAVR DRKY+IQTTYSHKFLG
Sbjct: 61  SVEEDCSSRLLYSYSNAMEGFAAQLSETELEYLKKLPDVVAVRADRKYEIQTTYSHKFLG 120

Query: 121 LSVGTQGVWQKSSMGQGAIVGLLDTGVWPESPSFSDSKMPPIPQKWRGACQEGQDFNSSN 180
           LSV TQGV  KSSMGQGAIVG+LDTGVWPESPSFSDSKMPPIPQKWRG CQEGQDFNSS+
Sbjct: 121 LSVCTQGVCYKSSMGQGAIVGVLDTGVWPESPSFSDSKMPPIPQKWRGGCQEGQDFNSSS 180

Query: 181 CNRKLIGAKFFIKGHHVASSLPSDVVQEYVSPRDSHGHGTHTSSTAAGASVADASVFGNG 240
           CNRKLIGAKFFIKGHHVASS PSD+VQEYVSPRDSHGHGTHTSSTAAGA VA ASVFGNG
Sbjct: 181 CNRKLIGAKFFIKGHHVASSPPSDIVQEYVSPRDSHGHGTHTSSTAAGALVAGASVFGNG 240

Query: 241 AGVAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAISDGVDILSLSLGGFPLPFFDDSI 300
            GVAQGMAPG HIAVYKVCWFSGCYSSDIVAAMDSAI DGVDILSLSLGGFPLPFFDDSI
Sbjct: 241 VGVAQGMAPGTHIAVYKVCWFSGCYSSDIVAAMDSAIRDGVDILSLSLGGFPLPFFDDSI 300

Query: 301 AIG----------IVCAAGNNGPTQSSVANVAPWITTIGAGTLDRRFPAIVRLSNGEAIY 360
           AIG          ++CAAGNNGPTQSSVANVAPWITTIGAGTLDR FPAIVRLSNGE I 
Sbjct: 301 AIGSFRAMQHGISVICAAGNNGPTQSSVANVAPWITTIGAGTLDRTFPAIVRLSNGEPIP 360

Query: 361 GESMYPGNKFKQATKELEVVYLTGGQMGGELCLKGSLPREKVQGKMVVCDRGVNGRSEKG 420
           GESMYPGNKF QATKELE+VYLTGGQMG ELCLKGSLPRE+V+GKMVVCDRGVNGRSEKG
Sbjct: 361 GESMYPGNKFNQATKELELVYLTGGQMGSELCLKGSLPREQVEGKMVVCDRGVNGRSEKG 420

Query: 421 QIVKESGGAAMILANSEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTNPKARIQFG 480
           QIVKESGGAAMILAN+EINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTNPKARIQFG
Sbjct: 421 QIVKESGGAAMILANTEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTNPKARIQFG 480

Query: 481 GTVIGRSRAPSVAQFSSRGPSLSNPSTLKPDVIAPGVNIIAAWPQNLGPTSLPEDSRRSN 540
           GTVIGR+RAPSVAQFSSRGPS +NPSTLKPDVIAPGVNIIAAWPQNL PT L EDSRRSN
Sbjct: 481 GTVIGRTRAPSVAQFSSRGPSPTNPSTLKPDVIAPGVNIIAAWPQNLAPTGLLEDSRRSN 540

Query: 541 FTVMSGTSMACPHVSGITALIHSAHPKWTPAAIKSAIMTTADVTDHFGKQILDGNKPADV 600
           FTVMSGTSMACPHVSGI ALI SAHPKWTPAAIKSAIMTTADVTD FGK ILDGNKPA V
Sbjct: 541 FTVMSGTSMACPHVSGIAALIQSAHPKWTPAAIKSAIMTTADVTDRFGKPILDGNKPASV 600

Query: 601 FAMGAGHVNPTKAIDPGLVYDIKPYEYIIHLCALGYTHSEIFIITHMNVSCHKVLQMNKG 660
           FAMGAGHVNPT+AIDPGLVYDIKP++Y+IHLC LGYTHSEIFIITHMNVSCHKVLQMNKG
Sbjct: 601 FAMGAGHVNPTRAIDPGLVYDIKPFDYVIHLCVLGYTHSEIFIITHMNVSCHKVLQMNKG 660

Query: 661 FTLNYPSMSVIFKHGTTSKMVSRRLTNVGSTNSIYEVKVTAPEGVRVRVKPRRLVFKHVN 720
           FTLNYPSM+V+FKHGTTSKMVSRRLTNVGS NS+YEV+VTAPEGV VRVKPRRLVFKHVN
Sbjct: 661 FTLNYPSMAVVFKHGTTSKMVSRRLTNVGSPNSVYEVEVTAPEGVSVRVKPRRLVFKHVN 720

Query: 721 QSLNYKVWFIEVYRRD----------LTWIHCENSKYKVRSPIVVTWKN 749
           QSL+YKVWF+   RR+          LTW+H ENSKY+VRSPIVVTWKN
Sbjct: 721 QSLSYKVWFMSEKRREGRKVSSTEGHLTWLHSENSKYRVRSPIVVTWKN 769

BLAST of IVF0024075 vs. NCBI nr
Match: XP_008447369.1 (PREDICTED: subtilisin-like protease SBT1.2 [Cucumis melo] >KAA0037948.1 subtilisin-like protease SBT1.2 [Cucumis melo var. makuwa] >TYK19024.1 subtilisin-like protease SBT1.2 [Cucumis melo var. makuwa])

HSP 1 Score: 1469 bits (3803), Expect = 0.0
Identity = 741/768 (96.48%), Postives = 743/768 (96.74%), Query Frame = 0

Query: 1   MDFNTRMGFLLFLYFLSLLVQPNTSTLQTYIIQLHPHGLITSVFDSKLQWHLSFLEQTLS 60
           MDFNTRMGFLLFLYFLSLLVQPNTSTLQTYIIQLHPHGLITSVFDSKLQWHLSFLEQTLS
Sbjct: 1   MDFNTRMGFLLFLYFLSLLVQPNTSTLQTYIIQLHPHGLITSVFDSKLQWHLSFLEQTLS 60

Query: 61  AEEDSSSRLLYSYSNAMEGFAAQLTETELEYLKKLPDVVAVREDRKYQIQTTYSHKFLGL 120
           AEEDSSSRLLYSYSNAMEGFAAQLTETELEYLKKLPDVVAVREDRKYQIQTTYSHKFLGL
Sbjct: 61  AEEDSSSRLLYSYSNAMEGFAAQLTETELEYLKKLPDVVAVREDRKYQIQTTYSHKFLGL 120

Query: 121 SVGTQGVWQKSSMGQGAIVGLLDTGVWPESPSFSDSKMPPIPQKWRGACQEGQDFNSSNC 180
           SVGTQGVWQKSSMGQGAIVGLLDTGVWPESPSFSDSKMPPIPQKWRGACQEGQDFNSSNC
Sbjct: 121 SVGTQGVWQKSSMGQGAIVGLLDTGVWPESPSFSDSKMPPIPQKWRGACQEGQDFNSSNC 180

Query: 181 NRKLIGAKFFIKGHHVASSLPSDVVQEYVSPRDSHGHGTHTSSTAAGASVADASVFGNGA 240
           NRKLIGAKFFIKGHHVASSLPSDVVQEYVSPRDSHGHGTHTSSTAAGASVADASVFGNGA
Sbjct: 181 NRKLIGAKFFIKGHHVASSLPSDVVQEYVSPRDSHGHGTHTSSTAAGASVADASVFGNGA 240

Query: 241 GVAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAISDGVDILSLSLGGFPLPFFDDSIA 300
           GVAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAISDGVDILSLSLGGFPLPFFDDSIA
Sbjct: 241 GVAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAISDGVDILSLSLGGFPLPFFDDSIA 300

Query: 301 IG----------IVCAAGNNGPTQSSVANVAPWITTIGAGTLDRRFPAIVRLSNGEAIYG 360
           IG          +VCAAGNNGPTQSSVANVAPWITTIGAGTLDRRFPAIVRLSNGEAIYG
Sbjct: 301 IGSFRAMQHGISVVCAAGNNGPTQSSVANVAPWITTIGAGTLDRRFPAIVRLSNGEAIYG 360

Query: 361 ESMYPGNKFKQATKELEVVYLTGGQMGGELCLKGSLPREKVQGKMVVCDRGVNGRSEKGQ 420
           ESMYPGNKFKQATKELEVVYLTGGQMGGELCLKGSLPREKVQGKMVVCDRGVNGRSEKGQ
Sbjct: 361 ESMYPGNKFKQATKELEVVYLTGGQMGGELCLKGSLPREKVQGKMVVCDRGVNGRSEKGQ 420

Query: 421 IVKESGGAAMILANSEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTNPKARIQFGG 480
           IVKESGGAAMILANSEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTNPKARIQFGG
Sbjct: 421 IVKESGGAAMILANSEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTNPKARIQFGG 480

Query: 481 TVIGRSRAPSVAQFSSRGPSLSNPSTLKPDVIAPGVNIIAAWPQNLGPTSLPEDSRRSNF 540
           TVIGRSRAPSVAQFSSRGPSLSNPSTLKPDVIAPGVNIIAAWPQNLGPTSLPEDSRRSNF
Sbjct: 481 TVIGRSRAPSVAQFSSRGPSLSNPSTLKPDVIAPGVNIIAAWPQNLGPTSLPEDSRRSNF 540

Query: 541 TVMSGTSMACPHVSGITALIHSAHPKWTPAAIKSAIMTTADVTDHFGKQILDGNKPADVF 600
           TVMSGTSMACPHVSGITALIHSAHPKWTPAAIKSAIMTTADVTDHFGKQILDGNKPADVF
Sbjct: 541 TVMSGTSMACPHVSGITALIHSAHPKWTPAAIKSAIMTTADVTDHFGKQILDGNKPADVF 600

Query: 601 AMGAGHVNPTKAIDPGLVYDIKPYEYIIHLCALGYTHSEIFIITHMNVSCHKVLQMNKGF 660
           AMGAGHVNPTKAIDPGLVYDIKPYEYIIHLCALGYTHSEIFIITHMNVSCHKVLQMNKGF
Sbjct: 601 AMGAGHVNPTKAIDPGLVYDIKPYEYIIHLCALGYTHSEIFIITHMNVSCHKVLQMNKGF 660

Query: 661 TLNYPSMSVIFKHGTTSKMVSRRLTNVGSTNSIYEVKVTAPEGVRVRVKPRRLVFKHVNQ 720
           TLNYPSMSVIFKHGTTSKMVSRRLTNVGSTNSIYEVKVTAPEGVRVRVKPRRLVFKHVNQ
Sbjct: 661 TLNYPSMSVIFKHGTTSKMVSRRLTNVGSTNSIYEVKVTAPEGVRVRVKPRRLVFKHVNQ 720

Query: 721 SLNYKVWF----------IEVYRRDLTWIHCENSKYKVRSPIVVTWKN 748
           SLNYKVWF          +     DLTWIHCENSKYKVRSPIVVTWKN
Sbjct: 721 SLNYKVWFMSEKGKEGRKVRFTEGDLTWIHCENSKYKVRSPIVVTWKN 768

BLAST of IVF0024075 vs. NCBI nr
Match: XP_011651620.1 (subtilisin-like protease SBT1.2 [Cucumis sativus] >KGN58306.1 hypothetical protein Csa_017640 [Cucumis sativus])

HSP 1 Score: 1440 bits (3728), Expect = 0.0
Identity = 724/768 (94.27%), Postives = 736/768 (95.83%), Query Frame = 0

Query: 1   MDFNTRMGFLLFLYFLSLLVQPNTSTLQTYIIQLHPHGLITSVFDSKLQWHLSFLEQTLS 60
           MDFN RMGFLLFL FLSLLVQPNTSTLQTYIIQLHPHGLITSVFDSKLQWHLSFLEQ+LS
Sbjct: 1   MDFNARMGFLLFLCFLSLLVQPNTSTLQTYIIQLHPHGLITSVFDSKLQWHLSFLEQSLS 60

Query: 61  AEEDSSSRLLYSYSNAMEGFAAQLTETELEYLKKLPDVVAVREDRKYQIQTTYSHKFLGL 120
           AEEDSSSRLLYSYSNAMEGFAAQL+ETELEYLK+LPDVVAVREDRKYQIQTTYSHKFLGL
Sbjct: 61  AEEDSSSRLLYSYSNAMEGFAAQLSETELEYLKRLPDVVAVREDRKYQIQTTYSHKFLGL 120

Query: 121 SVGTQGVWQKSSMGQGAIVGLLDTGVWPESPSFSDSKMPPIPQKWRGACQEGQDFNSSNC 180
           SVGTQG+ QKSSMGQGAIVG+LDTGVWPESPSFSDSKMPP+PQKWRGACQEGQDFNSSNC
Sbjct: 121 SVGTQGLRQKSSMGQGAIVGVLDTGVWPESPSFSDSKMPPVPQKWRGACQEGQDFNSSNC 180

Query: 181 NRKLIGAKFFIKGHHVASSLPSDVVQEYVSPRDSHGHGTHTSSTAAGASVADASVFGNGA 240
           NRKLIGAKFFIKGHHVASSLPSDV QEYVSPRDSHGHGTHTSSTAAGASVADASVFGNGA
Sbjct: 181 NRKLIGAKFFIKGHHVASSLPSDVAQEYVSPRDSHGHGTHTSSTAAGASVADASVFGNGA 240

Query: 241 GVAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAISDGVDILSLSLGGFPLPFFDDSIA 300
           GVAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAI DGVDILSLSLGGFPLPFFDDSIA
Sbjct: 241 GVAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAIRDGVDILSLSLGGFPLPFFDDSIA 300

Query: 301 IG----------IVCAAGNNGPTQSSVANVAPWITTIGAGTLDRRFPAIVRLSNGEAIYG 360
           IG          +VCAAGNNGP QSSVANVAPWITTIGAGTLDRRFPAI+RLSNGEAIYG
Sbjct: 301 IGSFRAMQHGISVVCAAGNNGPIQSSVANVAPWITTIGAGTLDRRFPAIIRLSNGEAIYG 360

Query: 361 ESMYPGNKFKQATKELEVVYLTGGQMGGELCLKGSLPREKVQGKMVVCDRGVNGRSEKGQ 420
           ESMYPGNKFKQATKELEVVYLTGGQMGGELCLKGSLPREKVQGKMVVCDRGVNGRSEKGQ
Sbjct: 361 ESMYPGNKFKQATKELEVVYLTGGQMGGELCLKGSLPREKVQGKMVVCDRGVNGRSEKGQ 420

Query: 421 IVKESGGAAMILANSEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTNPKARIQFGG 480
           IVKESGGAAMILANSEINLEEDLVDVHVLPATLIGFAEANRLKAYINTT+NPKARIQFGG
Sbjct: 421 IVKESGGAAMILANSEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTSNPKARIQFGG 480

Query: 481 TVIGRSRAPSVAQFSSRGPSLSNPSTLKPDVIAPGVNIIAAWPQNLGPTSLPEDSRRSNF 540
           TVIGRSRAPSVAQFSSRGPSLSNPSTLKPDVIAPGVNIIAAWPQNLGPT LPEDSRRSNF
Sbjct: 481 TVIGRSRAPSVAQFSSRGPSLSNPSTLKPDVIAPGVNIIAAWPQNLGPTGLPEDSRRSNF 540

Query: 541 TVMSGTSMACPHVSGITALIHSAHPKWTPAAIKSAIMTTADVTDHFGKQILDGNKPADVF 600
           TVMSGTSMACPHVSGITALIHSAHPKWTPAAIKSAIMTTADVTDHFGKQILDGNKPADVF
Sbjct: 541 TVMSGTSMACPHVSGITALIHSAHPKWTPAAIKSAIMTTADVTDHFGKQILDGNKPADVF 600

Query: 601 AMGAGHVNPTKAIDPGLVYDIKPYEYIIHLCALGYTHSEIFIITHMNVSCHKVLQMNKGF 660
           AMGAGHVNPTKAIDPGLVYDIKPYEYIIHLCALGYTHSEIFIITHMNVSCHK+LQMNKGF
Sbjct: 601 AMGAGHVNPTKAIDPGLVYDIKPYEYIIHLCALGYTHSEIFIITHMNVSCHKILQMNKGF 660

Query: 661 TLNYPSMSVIFKHGTTSKMVSRRLTNVGSTNSIYEVKVTAPEGVRVRVKPRRLVFKHVNQ 720
           TLNYPS+SVIFKHGTTSKMVSRRLTNVGSTNSIYEVKVTAPEGVRVRVKPRRLVFKHVNQ
Sbjct: 661 TLNYPSISVIFKHGTTSKMVSRRLTNVGSTNSIYEVKVTAPEGVRVRVKPRRLVFKHVNQ 720

Query: 721 SLNYKVWF----------IEVYRRDLTWIHCENSKYKVRSPIVVTWKN 748
           SLNYKVWF          +     DLTWIHCENSKYKVRSPIVVTWKN
Sbjct: 721 SLNYKVWFMSEKGKEGRKVRFTEGDLTWIHCENSKYKVRSPIVVTWKN 768

BLAST of IVF0024075 vs. NCBI nr
Match: XP_038887438.1 (subtilisin-like protease SBT1.2 [Benincasa hispida])

HSP 1 Score: 1385 bits (3586), Expect = 0.0
Identity = 697/769 (90.64%), Postives = 720/769 (93.63%), Query Frame = 0

Query: 1   MDFNTRMGFLLFLYFLSLLVQPNTSTLQTYIIQLHPHGLITSVFDSKLQWHLSFLEQTLS 60
           MDFNT++GFLLFL FLSL VQ NT+ LQTYIIQLHPHGL TS++DSKLQWHLSFLE+TLS
Sbjct: 1   MDFNTQLGFLLFLCFLSLHVQSNTTALQTYIIQLHPHGLTTSLYDSKLQWHLSFLERTLS 60

Query: 61  AEEDSSSRLLYSYSNAMEGFAAQLTETELEYLKKLPDVVAVREDRKYQIQTTYSHKFLGL 120
            EEDSSSRLLYSYSNAMEGFAAQL+ETELEYLKKLPDVVAVR DRKYQIQTTYSHKFLGL
Sbjct: 61  VEEDSSSRLLYSYSNAMEGFAAQLSETELEYLKKLPDVVAVRADRKYQIQTTYSHKFLGL 120

Query: 121 SVGTQGVWQKSSMGQGAIVGLLDTGVWPESPSFSDSKMPPIPQKWRGACQEGQDFNSSNC 180
           SVG QGVWQKSSMGQGAIVG+LDTGVWPESPSFSD KMPPIP KWRGACQEGQDFNSSNC
Sbjct: 121 SVGRQGVWQKSSMGQGAIVGVLDTGVWPESPSFSDYKMPPIPPKWRGACQEGQDFNSSNC 180

Query: 181 NRKLIGAKFFIKGHHVASSLPSDVVQEYVSPRDSHGHGTHTSSTAAGASVADASVFGNGA 240
           NRKLIGAKFFIKGHHVASS PSDVVQEYVSPRDSHGHGTHTSSTAAGASVA+ASVFGNGA
Sbjct: 181 NRKLIGAKFFIKGHHVASSPPSDVVQEYVSPRDSHGHGTHTSSTAAGASVAEASVFGNGA 240

Query: 241 GVAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAISDGVDILSLSLGGFPLPFFDDSIA 300
           GVAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAI DGVDILSLSLGGFPLPFFDDSIA
Sbjct: 241 GVAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAIKDGVDILSLSLGGFPLPFFDDSIA 300

Query: 301 IG----------IVCAAGNNGPTQSSVANVAPWITTIGAGTLDRRFPAIVRLSNGEAIYG 360
           IG          +VCAAGNNGPTQSSVANVAPWITTIGAGTLDRRFPAIV+LSNGE IYG
Sbjct: 301 IGSFRAMQHGISVVCAAGNNGPTQSSVANVAPWITTIGAGTLDRRFPAIVQLSNGETIYG 360

Query: 361 ESMYPGNKFKQATKELEVVYLTGGQMGGELCLKGSLPREKVQGKMVVCDRGVNGRSEKGQ 420
           ESMYPGNKFKQATKELEVVYLTGGQMGGELCLKGSLPRE+VQGKMVVCDRGVNGRSEKGQ
Sbjct: 361 ESMYPGNKFKQATKELEVVYLTGGQMGGELCLKGSLPREQVQGKMVVCDRGVNGRSEKGQ 420

Query: 421 IVKESGGAAMILANSEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTNPKARIQFGG 480
           IVKESGGAAMILANSEINLEEDLVDVHVLPATLIGFAEANRLK+YINTTTNPKARIQFGG
Sbjct: 421 IVKESGGAAMILANSEINLEEDLVDVHVLPATLIGFAEANRLKSYINTTTNPKARIQFGG 480

Query: 481 TVIGRSRAPSVAQFSSRGPSLSNPSTLKPDVIAPGVNIIAAWPQNLGPTSLPEDSRRSNF 540
           TVIGRSRAPSVAQFSSRGPSLSNPSTLKPDVIAPGVNIIAAWPQNLGPT LP+DSR SNF
Sbjct: 481 TVIGRSRAPSVAQFSSRGPSLSNPSTLKPDVIAPGVNIIAAWPQNLGPTGLPDDSRSSNF 540

Query: 541 TVMSGTSMACPHVSGITALIHSAHPKWTPAAIKSAIMTTADVTDHFGKQILDGN-KPADV 600
           TVMSGTSMACPHVSGI ALIHS HP WTPAAIKSAIMTTADVTDHFGK ILDGN KPA V
Sbjct: 541 TVMSGTSMACPHVSGIAALIHSVHPNWTPAAIKSAIMTTADVTDHFGKPILDGNNKPAGV 600

Query: 601 FAMGAGHVNPTKAIDPGLVYDIKPYEYIIHLCALGYTHSEIFIITHMNVSCHKVLQMNKG 660
           FAMGAGHVNPTKAIDPGL+YDIKPYEYIIHLCALGYTHSEIFI+THMNVSCHKV+QMNKG
Sbjct: 601 FAMGAGHVNPTKAIDPGLIYDIKPYEYIIHLCALGYTHSEIFIVTHMNVSCHKVVQMNKG 660

Query: 661 FTLNYPSMSVIFKHGTTSKMVSRRLTNVGSTNSIYEVKVTAPEGVRVRVKPRRLVFKHVN 720
           FTLNYPSMSVIFKHGTTSKMVSRRLTNVG+ NSIY+V+V APEGVRVRVKPRRLVFKHVN
Sbjct: 661 FTLNYPSMSVIFKHGTTSKMVSRRLTNVGTPNSIYKVEVAAPEGVRVRVKPRRLVFKHVN 720

Query: 721 QSLNYKVWFIEVYRRD----------LTWIHCENSKYKVRSPIVVTWKN 748
           QSLNYKVWF     ++          LTW+H ENSKYKVRSPIVVTWKN
Sbjct: 721 QSLNYKVWFTSEKGKEGRKVSSTEGHLTWLHSENSKYKVRSPIVVTWKN 769

BLAST of IVF0024075 vs. NCBI nr
Match: XP_022963543.1 (subtilisin-like protease SBT1.2 [Cucurbita moschata])

HSP 1 Score: 1334 bits (3453), Expect = 0.0
Identity = 674/769 (87.65%), Postives = 706/769 (91.81%), Query Frame = 0

Query: 1   MDFNTRMGF-LLFLYFLSLLVQPNTSTLQTYIIQLHPHGLITSVFDSKLQWHLSFLEQTL 60
           MDF ++M F  +FL FL L VQ N S LQTY+IQLHP GL +SVF+SKLQWHLSFLEQTL
Sbjct: 1   MDFYSQMVFSTIFLCFLLLQVQANASNLQTYMIQLHPQGLTSSVFESKLQWHLSFLEQTL 60

Query: 61  SAEEDSSSRLLYSYSNAMEGFAAQLTETELEYLKKLPDVVAVREDRKYQIQTTYSHKFLG 120
           S EED SSRLLYSYSNAMEGFAAQL+ETELEYLKKLPDVVAVR DRKY+IQTTYSHKFLG
Sbjct: 61  SVEEDCSSRLLYSYSNAMEGFAAQLSETELEYLKKLPDVVAVRADRKYEIQTTYSHKFLG 120

Query: 121 LSVGTQGVWQKSSMGQGAIVGLLDTGVWPESPSFSDSKMPPIPQKWRGACQEGQDFNSSN 180
           LSV TQGV  KSSMGQGAIVG+LDTGVWPESPSF DSKMPPIPQKWRG CQEGQDFNSS+
Sbjct: 121 LSVCTQGVCYKSSMGQGAIVGVLDTGVWPESPSFGDSKMPPIPQKWRGGCQEGQDFNSSS 180

Query: 181 CNRKLIGAKFFIKGHHVASSLPSDVVQEYVSPRDSHGHGTHTSSTAAGASVADASVFGNG 240
           CNRKLIGAKFFIKGHHVASS PSD VQEYVSPRDSHGHGTHTSSTAAGASVA ASVFGNG
Sbjct: 181 CNRKLIGAKFFIKGHHVASSPPSDTVQEYVSPRDSHGHGTHTSSTAAGASVAGASVFGNG 240

Query: 241 AGVAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAISDGVDILSLSLGGFPLPFFDDSI 300
           AGVAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAI DGVDILSLSLGGFPLPFFDDSI
Sbjct: 241 AGVAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAIRDGVDILSLSLGGFPLPFFDDSI 300

Query: 301 AIG----------IVCAAGNNGPTQSSVANVAPWITTIGAGTLDRRFPAIVRLSNGEAIY 360
           AIG          ++CAAGNNGPTQSSVANVAPWITTIGAGTLDR FPAIVRLSNGEAI 
Sbjct: 301 AIGSFRAMQHGISVICAAGNNGPTQSSVANVAPWITTIGAGTLDRTFPAIVRLSNGEAIP 360

Query: 361 GESMYPGNKFKQATKELEVVYLTGGQMGGELCLKGSLPREKVQGKMVVCDRGVNGRSEKG 420
           GESMYPGNKF QATKELE+VYLTGGQMG ELCLKGSLPRE+V+GKMVVCDRGVNGRSEKG
Sbjct: 361 GESMYPGNKFNQATKELELVYLTGGQMGSELCLKGSLPREQVEGKMVVCDRGVNGRSEKG 420

Query: 421 QIVKESGGAAMILANSEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTNPKARIQFG 480
           QIVKESGGAAMILAN+EINLEEDLVDVHVLPATLIGFAEANRLKAYINTTT+PKARIQFG
Sbjct: 421 QIVKESGGAAMILANTEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTDPKARIQFG 480

Query: 481 GTVIGRSRAPSVAQFSSRGPSLSNPSTLKPDVIAPGVNIIAAWPQNLGPTSLPEDSRRSN 540
           GTVIGR+RAPSVAQFSSRGPS +NPSTLKPDVIAPGVNIIAAWPQNL PT L EDSRRSN
Sbjct: 481 GTVIGRTRAPSVAQFSSRGPSPTNPSTLKPDVIAPGVNIIAAWPQNLAPTGLLEDSRRSN 540

Query: 541 FTVMSGTSMACPHVSGITALIHSAHPKWTPAAIKSAIMTTADVTDHFGKQILDGNKPADV 600
           FTVMSGTSM+CPHVSGI ALIHSAHPKWTPAAIKSAIMTTADVTD FGK ILDGNKPA V
Sbjct: 541 FTVMSGTSMSCPHVSGIAALIHSAHPKWTPAAIKSAIMTTADVTDRFGKPILDGNKPASV 600

Query: 601 FAMGAGHVNPTKAIDPGLVYDIKPYEYIIHLCALGYTHSEIFIITHMNVSCHKVLQMNKG 660
           FAMGAGHVNPT+AIDPGLVYDIKPY+Y+IHLC LGYTHSEIFIITHMNVSCHKVLQMNKG
Sbjct: 601 FAMGAGHVNPTRAIDPGLVYDIKPYDYVIHLCVLGYTHSEIFIITHMNVSCHKVLQMNKG 660

Query: 661 FTLNYPSMSVIFKHGTTSKMVSRRLTNVGSTNSIYEVKVTAPEGVRVRVKPRRLVFKHVN 720
           FTLNYPSMSV+FKHGTTSKMVSRRLTNVGS NS+YEV+VTAPEGV VRVKPRRLVFKHVN
Sbjct: 661 FTLNYPSMSVVFKHGTTSKMVSRRLTNVGSPNSVYEVEVTAPEGVSVRVKPRRLVFKHVN 720

Query: 721 QSLNYKVWFIEVYRRD----------LTWIHCENSKYKVRSPIVVTWKN 748
           +SL+YKVWF+   RR+          LTW+H ENSKY+VRSPIVVTWKN
Sbjct: 721 ESLSYKVWFMSEKRREGRKVSSTEGHLTWLHSENSKYRVRSPIVVTWKN 769

BLAST of IVF0024075 vs. NCBI nr
Match: XP_023553603.1 (subtilisin-like protease SBT1.2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1332 bits (3446), Expect = 0.0
Identity = 671/769 (87.26%), Postives = 708/769 (92.07%), Query Frame = 0

Query: 1   MDFNTRMGF-LLFLYFLSLLVQPNTSTLQTYIIQLHPHGLITSVFDSKLQWHLSFLEQTL 60
           MDFN++MGF  +FL FL L VQ N S LQTY+IQLHP GL +SVF+SKLQWHLSFLEQTL
Sbjct: 1   MDFNSQMGFPTIFLCFLLLQVQANASNLQTYMIQLHPQGLTSSVFESKLQWHLSFLEQTL 60

Query: 61  SAEEDSSSRLLYSYSNAMEGFAAQLTETELEYLKKLPDVVAVREDRKYQIQTTYSHKFLG 120
           S EED SSRLLYSYSNAMEGFAAQL+ETELEYLKKLPDVVAVR DRKY+IQTTYSHKFLG
Sbjct: 61  SVEEDCSSRLLYSYSNAMEGFAAQLSETELEYLKKLPDVVAVRADRKYEIQTTYSHKFLG 120

Query: 121 LSVGTQGVWQKSSMGQGAIVGLLDTGVWPESPSFSDSKMPPIPQKWRGACQEGQDFNSSN 180
             V TQGV  KSSMGQGAIVG+LDTGVWPESPSFSDSKMPPIPQKWRG CQEGQDFNSS+
Sbjct: 121 --VCTQGVCYKSSMGQGAIVGVLDTGVWPESPSFSDSKMPPIPQKWRGGCQEGQDFNSSS 180

Query: 181 CNRKLIGAKFFIKGHHVASSLPSDVVQEYVSPRDSHGHGTHTSSTAAGASVADASVFGNG 240
           CNRKLIGAKFFIKGHHVASS PSD VQEYVSPRDSHGHGTHTSSTAAGASVA ASVFGNG
Sbjct: 181 CNRKLIGAKFFIKGHHVASSPPSDTVQEYVSPRDSHGHGTHTSSTAAGASVAGASVFGNG 240

Query: 241 AGVAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAISDGVDILSLSLGGFPLPFFDDSI 300
           AGVAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAI DGVDILSLSLGGFPLPFFDD+I
Sbjct: 241 AGVAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAIRDGVDILSLSLGGFPLPFFDDNI 300

Query: 301 AIG----------IVCAAGNNGPTQSSVANVAPWITTIGAGTLDRRFPAIVRLSNGEAIY 360
           AIG          ++CAAGNNGPTQSSVANVAPWITTIGAGTLDR FPAIVRLSNG+AI 
Sbjct: 301 AIGSFRAVQHGISVICAAGNNGPTQSSVANVAPWITTIGAGTLDRTFPAIVRLSNGKAIP 360

Query: 361 GESMYPGNKFKQATKELEVVYLTGGQMGGELCLKGSLPREKVQGKMVVCDRGVNGRSEKG 420
           GESMYPGNKF QATKELE+VYLTGGQMG ELCLKGSLPRE+V+GKMVVCDRGVNGRSEKG
Sbjct: 361 GESMYPGNKFNQATKELELVYLTGGQMGSELCLKGSLPREQVEGKMVVCDRGVNGRSEKG 420

Query: 421 QIVKESGGAAMILANSEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTNPKARIQFG 480
           QIVKESGGAAMILAN+EINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTNPKARIQFG
Sbjct: 421 QIVKESGGAAMILANTEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTNPKARIQFG 480

Query: 481 GTVIGRSRAPSVAQFSSRGPSLSNPSTLKPDVIAPGVNIIAAWPQNLGPTSLPEDSRRSN 540
           GTVIGR+RAPSVAQFSSRGPS +NPSTLKPDVIAPGVNIIAAWPQNL PT LPEDSRRSN
Sbjct: 481 GTVIGRTRAPSVAQFSSRGPSPTNPSTLKPDVIAPGVNIIAAWPQNLAPTGLPEDSRRSN 540

Query: 541 FTVMSGTSMACPHVSGITALIHSAHPKWTPAAIKSAIMTTADVTDHFGKQILDGNKPADV 600
           F+VMSGTSM+CPHVSGI ALIHSAHPKWTPAAIKSAIMTTADVTD FGK ILDGNKPA V
Sbjct: 541 FSVMSGTSMSCPHVSGIAALIHSAHPKWTPAAIKSAIMTTADVTDRFGKPILDGNKPASV 600

Query: 601 FAMGAGHVNPTKAIDPGLVYDIKPYEYIIHLCALGYTHSEIFIITHMNVSCHKVLQMNKG 660
           FAMGAGHVNPT+AIDPGLVYDIKPY+Y+IHLC LGYTHSEIFIITHMNVSCHKVLQMNKG
Sbjct: 601 FAMGAGHVNPTRAIDPGLVYDIKPYDYVIHLCVLGYTHSEIFIITHMNVSCHKVLQMNKG 660

Query: 661 FTLNYPSMSVIFKHGTTSKMVSRRLTNVGSTNSIYEVKVTAPEGVRVRVKPRRLVFKHVN 720
           FTLNYPSM+V+FKHGTTSKMVSR+LTNVGS NS+YEV+VTAPEGV VRVKP+RLVFKHVN
Sbjct: 661 FTLNYPSMAVVFKHGTTSKMVSRQLTNVGSPNSVYEVEVTAPEGVSVRVKPQRLVFKHVN 720

Query: 721 QSLNYKVWFIEVYRRD----------LTWIHCENSKYKVRSPIVVTWKN 748
           +SL+YKVWF+   RR+          LTW+H ENSKY+VRSPIVVTWKN
Sbjct: 721 ESLSYKVWFMSEKRREGRKVSSTEGHLTWLHSENSKYRVRSPIVVTWKN 767

BLAST of IVF0024075 vs. TAIR 10
Match: AT1G04110.1 (Subtilase family protein )

HSP 1 Score: 1011.1 bits (2613), Expect = 4.5e-295
Identity = 503/767 (65.58%), Postives = 611/767 (79.66%), Query Frame = 0

Query: 9   FLLFLYFLSLLVQPNTSTLQTYIIQLHPHGLITSVFDSKLQWHLSFLEQTL----SAEED 68
           FLLF    S ++Q      QTYI+QLHP+      F SK  WHLSFL++ +      EE+
Sbjct: 12  FLLFCSSSSEILQK-----QTYIVQLHPNSETAKTFASKFDWHLSFLQEAVLGVEEEEEE 71

Query: 69  SSSRLLYSYSNAMEGFAAQLTETELEYLKKLPDVVAVREDRKYQIQTTYSHKFLGL-SVG 128
            SSRLLYSY +A+EGFAAQLTE+E E L+  P+VVAVR D   Q+QTTYS+KFLGL   G
Sbjct: 72  PSSRLLYSYGSAIEGFAAQLTESEAEILRYSPEVVAVRPDHVLQVQTTYSYKFLGLDGFG 131

Query: 129 TQGVWQKSSMGQGAIVGLLDTGVWPESPSFSDSKMPPIPQKWRGACQEGQDFNSSNCNRK 188
             GVW KS  GQG I+G+LDTGVWPESPSF D+ MP IP+KW+G CQEG+ F+SS+CNRK
Sbjct: 132 NSGVWSKSRFGQGTIIGVLDTGVWPESPSFDDTGMPSIPRKWKGICQEGESFSSSSCNRK 191

Query: 189 LIGAKFFIKGHHVASSLPS--DVVQEYVSPRDSHGHGTHTSSTAAGASVADASVFGNGAG 248
           LIGA+FFI+GH VA+S     ++ +EY+S RDS GHGTHT+ST  G+SV+ A+V GNGAG
Sbjct: 192 LIGARFFIRGHRVANSPEESPNMPREYISARDSTGHGTHTASTVGGSSVSMANVLGNGAG 251

Query: 249 VAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAISDGVDILSLSLGGFPLPFFDDSIAI 308
           VA+GMAPGAHIAVYKVCWF+GCYSSDI+AA+D AI D VD+LSLSLGGFP+P +DD+IAI
Sbjct: 252 VARGMAPGAHIAVYKVCWFNGCYSSDILAAIDVAIQDKVDVLSLSLGGFPIPLYDDTIAI 311

Query: 309 G----------IVCAAGNNGPTQSSVANVAPWITTIGAGTLDRRFPAIVRLSNGEAIYGE 368
           G          ++CAAGNNGP +SSVAN APW++TIGAGTLDRRFPA+VRL+NG+ +YGE
Sbjct: 312 GTFRAMERGISVICAAGNNGPIESSVANTAPWVSTIGAGTLDRRFPAVVRLANGKLLYGE 371

Query: 369 SMYPGNKFKQATKELEVVYLTGGQMGGELCLKGSLPREKVQGKMVVCDRGVNGRSEKGQI 428
           S+YPG   K A +E+EV+Y+TGG  G E CL+GSLPRE+++GKMV+CDRGVNGRSEKG+ 
Sbjct: 372 SLYPGKGIKNAGREVEVIYVTGGDKGSEFCLRGSLPREEIRGKMVICDRGVNGRSEKGEA 431

Query: 429 VKESGGAAMILANSEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTNPKARIQFGGT 488
           VKE+GG AMILAN+EIN EED +DVH+LPATLIG+ E+  LKAY+N T  PKARI FGGT
Sbjct: 432 VKEAGGVAMILANTEINQEEDSIDVHLLPATLIGYTESVLLKAYVNATVKPKARIIFGGT 491

Query: 489 VIGRSRAPSVAQFSSRGPSLSNPSTLKPDVIAPGVNIIAAWPQNLGPTSLPEDSRRSNFT 548
           VIGRSRAP VAQFS+RGPSL+NPS LKPD+IAPGVNIIAAWPQNLGPT LP DSRR NFT
Sbjct: 492 VIGRSRAPEVAQFSARGPSLANPSILKPDMIAPGVNIIAAWPQNLGPTGLPYDSRRVNFT 551

Query: 549 VMSGTSMACPHVSGITALIHSAHPKWTPAAIKSAIMTTADVTDHFGKQILDGNKPADVFA 608
           VMSGTSM+CPHVSGITALI SA+P W+PAAIKSA+MTTAD+ D  GK I DGNKPA VFA
Sbjct: 552 VMSGTSMSCPHVSGITALIRSAYPNWSPAAIKSALMTTADLYDRQGKAIKDGNKPAGVFA 611

Query: 609 MGAGHVNPTKAIDPGLVYDIKPYEYIIHLCALGYTHSEIFIITHMNVSCHKVLQMNKGFT 668
           +GAGHVNP KAI+PGLVY+I+P +YI +LC LG+T S+I  ITH NVSC+ +L+ N GF+
Sbjct: 612 IGAGHVNPQKAINPGLVYNIQPVDYITYLCTLGFTRSDILAITHKNVSCNGILRKNPGFS 671

Query: 669 LNYPSMSVIFKHGTTSKMVSRRLTNVGSTNSIYEVKVTAPEGVRVRVKPRRLVFKHVNQS 728
           LNYPS++VIFK G T++M++RR+TNVGS NSIY V V APEG++V V P+RLVFKHV+Q+
Sbjct: 672 LNYPSIAVIFKRGKTTEMITRRVTNVGSPNSIYSVNVKAPEGIKVIVNPKRLVFKHVDQT 731

Query: 729 LNYKVWFI-----------EVYRRDLTWIHCENSKYKVRSPIVVTWK 748
           L+Y+VWF+              +  LTW++  N   +VRSPI VT K
Sbjct: 732 LSYRVWFVLKKKNRGGKVASFAQGQLTWVNSHNLMQRVRSPISVTLK 773

BLAST of IVF0024075 vs. TAIR 10
Match: AT2G05920.1 (Subtilase family protein )

HSP 1 Score: 628.2 bits (1619), Expect = 8.2e-180
Identity = 350/752 (46.54%), Postives = 473/752 (62.90%), Query Frame = 0

Query: 15  FLSLLVQPNTSTLQTYIIQLHPHGLITSVFDSKLQWHLSFLEQTLSAEEDSSSRLLYSYS 74
           FL LL+  +T+  +TYII+++ H      F +   W+ S L        +S S LLY+Y+
Sbjct: 17  FLFLLL--HTTAKKTYIIRVN-HSDKPESFLTHHDWYTSQL--------NSESSLLYTYT 76

Query: 75  NAMEGFAAQLTETELE-YLKKLPDVVAVREDRKYQIQTTYSHKFLGLSVGTQGVWQKSSM 134
            +  GF+A L  TE +  L     ++ + ED  Y + TT + +FLGL+    GV    S 
Sbjct: 77  TSFHGFSAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLN-SEFGVHDLGSS 136

Query: 135 GQGAIVGLLDTGVWPESPSFSDSKMPPIPQKWRGACQEGQDFNSSNCNRKLIGAKFFIKG 194
             G I+G+LDTGVWPES SF D+ MP IP KW+G C+ G DF+S  CN+KLIGA+ F KG
Sbjct: 137 SNGVIIGVLDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARSFSKG 196

Query: 195 HHVASSLPSDVVQEYVSPRDSHGHGTHTSSTAAGASVADASVFGNGAGVAQGMAPGAHIA 254
             +AS       +E VSPRD  GHGTHTS+TAAG++V +AS  G  AG A+GMA  A +A
Sbjct: 197 FQMASGGGFSSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRARVA 256

Query: 255 VYKVCWFSGCYSSDIVAAMDSAISDGVDILSLSLGGFPLPFFDDSIAIG----------I 314
            YKVCW +GC+ SDI+AAMD AI DGVD+LSLSLGG   P++ D+IAIG          +
Sbjct: 257 TYKVCWSTGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFSAMERGVFV 316

Query: 315 VCAAGNNGPTQSSVANVAPWITTIGAGTLDRRFPAIVRLSNGEAIYGESMYPGNKFKQAT 374
            C+AGN+GPT++SVANVAPW+ T+GAGTLDR FPA   L NG+ + G S+Y G      T
Sbjct: 317 SCSAGNSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSG--VGMGT 376

Query: 375 KELEVVYLTGGQMGGELCLKGSLPREKVQGKMVVCDRGVNGRSEKGQIVKESGGAAMILA 434
           K LE+VY  G      LCL GSL    V+GK+VVCDRGVN R EKG +V+++GG  MI+A
Sbjct: 377 KPLELVYNKGNSSSSNLCLPGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDAGGLGMIMA 436

Query: 435 NSEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTNPKARIQFGGTVIGRSRAPSVAQ 494
           N+  + EE + D H+LPA  +G    + L+ Y+ + + P A + F GTV+    +P VA 
Sbjct: 437 NTAASGEELVADSHLLPAIAVGKKTGDLLREYVKSDSKPTALLVFKGTVLDVKPSPVVAA 496

Query: 495 FSSRGPSLSNPSTLKPDVIAPGVNIIAAWPQNLGPTSLPEDSRRSNFTVMSGTSMACPHV 554
           FSSRGP+   P  LKPDVI PGVNI+A W   +GPT L +DSRR+ F +MSGTSM+CPH+
Sbjct: 497 FSSRGPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMSCPHI 556

Query: 555 SGITALIHSAHPKWTPAAIKSAIMTTADVTDHFGKQILDG--NKPADVFAMGAGHVNPTK 614
           SG+  L+ +AHP+W+P+AIKSA+MTTA V D+    + D   N  ++ +A G+GHV+P K
Sbjct: 557 SGLAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAADNSLSNPYAHGSGHVDPQK 616

Query: 615 AIDPGLVYDIKPYEYIIHLCALGYTHSEIF-IITHMNVSCHKVLQMNKGFTLNYPSMSVI 674
           A+ PGLVYDI   EYI  LC+L YT   I  I+   +V+C K  + +    LNYPS SV+
Sbjct: 617 ALSPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVNCSK--KFSDPGQLNYPSFSVL 676

Query: 675 FKHGTTSKMVSRRLTNVGSTNSIYEVKVTAPEGVRVRVKPRRLVFKHVNQSLNYKVWFIE 734
           F  G      +R +TNVG+ +S+Y+V V     V + VKP +L FK V +   Y V F+ 
Sbjct: 677 F-GGKRVVRYTREVTNVGAASSVYKVTVNGAPSVGISVKPSKLSFKSVGEKKRYTVTFVS 736

Query: 735 ------VYRRDLTWIHCENSKYKVRSPIVVTW 747
                   + +   I   N +++VRSP+  +W
Sbjct: 737 KKGVSMTNKAEFGSITWSNPQHEVRSPVAFSW 751

BLAST of IVF0024075 vs. TAIR 10
Match: AT3G14240.1 (Subtilase family protein )

HSP 1 Score: 609.8 bits (1571), Expect = 3.0e-174
Identity = 338/781 (43.28%), Postives = 477/781 (61.08%), Query Frame = 0

Query: 7   MGFLLFLYFLSLLVQPNTSTLQ----TYIIQLHPHGLITSVFDSKLQWHLSFLEQTLSAE 66
           M F  + +FL  L  P++S       TYI+ +  H    S+F +   W+ S    +L++ 
Sbjct: 1   MAFFFYFFFLLTLSSPSSSASSSNSLTYIVHV-DHEAKPSIFPTHFHWYTS----SLASL 60

Query: 67  EDSSSRLLYSYSNAMEGFAAQLTETELEYLKKLPDVVAVREDRKYQIQTTYSHKFLGL-S 126
             S   ++++Y     GF+A+LT  +   L   P V++V  ++   + TT S +FLGL S
Sbjct: 61  TSSPPSIIHTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRS 120

Query: 127 VGTQGVWQKSSMGQGAIVGLLDTGVWPESPSFSDSKMPPIPQKWRGACQEGQDFNSSNCN 186
               G+ ++S  G   ++G++DTGVWPE PSF D  + P+P KW+G C   QDF  S CN
Sbjct: 121 TDKAGLLEESDFGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACN 180

Query: 187 RKLIGAKFFIKGHHVASSLPSDVVQEYVSPRDSHGHGTHTSSTAAGASVADASVFGNGAG 246
           RKL+GA+FF  G+   +   ++   E+ SPRDS GHGTHT+S +AG  V  AS  G   G
Sbjct: 181 RKLVGARFFCGGYEATNGKMNETT-EFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHG 240

Query: 247 VAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAISDGVDILSLSLGGFPLPFFDDSIAI 306
           VA GMAP A +A YKVCW SGCY SDI+AA D+A++DGVD++SLS+GG  +P++ D+IAI
Sbjct: 241 VAAGMAPKARLAAYKVCWNSGCYDSDILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAI 300

Query: 307 G----------IVCAAGNNGPTQSSVANVAPWITTIGAGTLDRRFPAIVRLSNGEAIYGE 366
           G          +  +AGN GP   +V NVAPW+TT+GAGT+DR FPA V+L NG+ I G 
Sbjct: 301 GAFGAIDRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGV 360

Query: 367 SMYPGNKFKQATKELEVVYLTGGQMGGE-----LCLKGSLPREKVQGKMVVCDRGVNGRS 426
           S+Y G       +   +VY  G  +GG+     LCL+GSL    V+GK+V+CDRG+N R+
Sbjct: 361 SVYGGPGLDPG-RMYPLVY-GGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRA 420

Query: 427 EKGQIVKESGGAAMILANSEINLEEDLVDVHVLPATLIGFAEANRLKAYIN------TTT 486
            KG+IV+++GG  MI+AN   + E  + D HVLPAT +G +  + ++ YI+      ++ 
Sbjct: 421 TKGEIVRKNGGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSK 480

Query: 487 NPKARIQFGGTVIGRSRAPSVAQFSSRGPSLSNPSTLKPDVIAPGVNIIAAWPQNLGPTS 546
           +P A I F GT +G   AP VA FS+RGP+   P  LKPDVIAPG+NI+AAWP  +GP+ 
Sbjct: 481 HPTATIVFKGTRLGIRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSG 540

Query: 547 LPEDSRRSNFTVMSGTSMACPHVSGITALIHSAHPKWTPAAIKSAIMTTADVTDHFGKQI 606
           +  D+RR+ F ++SGTSMACPHVSG+ AL+ +AHP W+PAAI+SA++TTA   D+ G+ +
Sbjct: 541 VTSDNRRTEFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPM 600

Query: 607 LDGN--KPADVFAMGAGHVNPTKAIDPGLVYDIKPYEYIIHLCALGYTHSEIFIITHMNV 666
           +D +    + V   G+GHV+PTKA+DPGLVYDI  Y+YI  LC   YT + I  IT    
Sbjct: 601 MDESTGNTSSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQA 660

Query: 667 SCHKVLQMNKGFTLNYPSMSVIFKHGTTSKMVS---RRLTNVGSTNSIYEVKVTAPEGVR 726
            C    +      LNYPS SV+F+    SKM +   R +TNVG ++S+YE+K+  P G  
Sbjct: 661 DCDGARRAGHVGNLNYPSFSVVFQQYGESKMSTHFIRTVTNVGDSDSVYEIKIRPPRGTT 720

Query: 727 VRVKPRRLVFKHVNQSLNYKV-----------WFIEVYRRDLTWIHCENSKYKVRSPIVV 746
           V V+P +L F+ V Q L++ V               V    + W    + K  V SP+VV
Sbjct: 721 VTVEPEKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVETGHIVW---SDGKRNVTSPLVV 770

BLAST of IVF0024075 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 604.7 bits (1558), Expect = 9.7e-173
Identity = 340/764 (44.50%), Postives = 467/764 (61.13%), Query Frame = 0

Query: 3   FNTRMGFLLFLYFLSLLVQPNTSTLQTYIIQLHPHGLITSVFDSKLQWHLSFLEQTLSAE 62
           F +   F L L      V  ++S   TYI+ +     + S FD    W+ S L       
Sbjct: 5   FLSSTAFFLLLCLGFCHVSSSSSDQGTYIVHM-AKSQMPSSFDLHSNWYDSSLRSI---- 64

Query: 63  EDSSSRLLYSYSNAMEGFAAQLTETELEYLKKLPDVVAVREDRKYQIQTTYSHKFLGLSV 122
              S+ LLY+Y NA+ GF+ +LT+ E + L   P V++V  + +Y++ TT +  FLGL  
Sbjct: 65  -SDSAELLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDE 124

Query: 123 GTQGVWQKSSMGQGAIVGLLDTGVWPESPSFSDSKMPPIPQKWRGACQEGQDFNSSNCNR 182
            T  ++ ++      +VG+LDTGVWPES S+SD    PIP  W+G C+ G +F +S CNR
Sbjct: 125 HTADLFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNR 184

Query: 183 KLIGAKFFIKGHHVASSLPSDVVQEYVSPRDSHGHGTHTSSTAAGASVADASVFGNGAGV 242
           KLIGA+FF +G+  ++  P D  +E  SPRD  GHGTHTSSTAAG+ V  AS+ G  +G 
Sbjct: 185 KLIGARFFARGYE-STMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGT 244

Query: 243 AQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAISDGVDILSLSLGGFPLPFFDDSIAIG 302
           A+GMAP A +AVYKVCW  GC+SSDI+AA+D AI+D V++LS+SLGG    ++ D +AIG
Sbjct: 245 ARGMAPRARVAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIG 304

Query: 303 ----------IVCAAGNNGPTQSSVANVAPWITTIGAGTLDRRFPAIVRLSNGEAIYGES 362
                     + C+AGN GP+ SS++NVAPWITT+GAGTLDR FPA+  L NG+   G S
Sbjct: 305 AFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVS 364

Query: 363 MYPGNKFKQATKELEVVYL--TGGQMGGELCLKGSLPREKVQGKMVVCDRGVNGRSEKGQ 422
           ++ G       K L  +Y         G LC+ G+L  EKV+GK+V+CDRG+N R +KG 
Sbjct: 365 LFKGEALPD--KLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGD 424

Query: 423 IVKESGGAAMILANSEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTNPKARIQFGG 482
           +VK +GG  MILAN+  N EE + D H+LPAT +G    + ++ Y+ T  NP A I   G
Sbjct: 425 VVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILG 484

Query: 483 TVIGRSRAPSVAQFSSRGPSLSNPSTLKPDVIAPGVNIIAAWPQNLGPTSLPEDSRRSNF 542
           TV+G   +P VA FSSRGP+   P+ LKPD+IAPGVNI+AAW    GPT L  DSRR  F
Sbjct: 485 TVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEF 544

Query: 543 TVMSGTSMACPHVSGITALIHSAHPKWTPAAIKSAIMTTADVTDHFGKQILD--GNKPAD 602
            ++SGTSM+CPHVSG+ AL+ S HP+W+PAAI+SA+MTTA  T   GK +LD    KP+ 
Sbjct: 545 NIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPST 604

Query: 603 VFAMGAGHVNPTKAIDPGLVYDIKPYEYIIHLCALGYTHSEIFIITHMNVSCHKVLQMNK 662
            F  GAGHV+PT A +PGL+YD+   +Y+  LCAL YT  +I  ++  N +C      + 
Sbjct: 605 PFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSYSV 664

Query: 663 GFTLNYPSMSVIFKHGTTSKMVSRRLTNVGSTNSIYEVKVTA-PEGVRVRVKPRRLVFKH 722
              LNYPS +V    G  +   +R +T+VG   + Y VKVT+   GV++ V+P  L FK 
Sbjct: 665 A-DLNYPSFAVNV-DGVGAYKYTRTVTSVGGAGT-YSVKVTSETTGVKISVEPAVLNFKE 724

Query: 723 VNQSLNYKVWFIEVYRR-----DLTWIHCENSKYKVRSPIVVTW 747
            N+  +Y V F     +         I   + K+ V SP+ ++W
Sbjct: 725 ANEKKSYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAISW 756

BLAST of IVF0024075 vs. TAIR 10
Match: AT5G51750.1 (subtilase 1.3 )

HSP 1 Score: 598.2 bits (1541), Expect = 9.1e-171
Identity = 329/760 (43.29%), Postives = 462/760 (60.79%), Query Frame = 0

Query: 13  LYFLSLLVQPNTSTLQTYIIQLHPHGLITSVFDSKLQWHLSFL----EQTLSAEEDSSSR 72
           L FL        ST +TY+I +    +    + + LQW+ S +    +     EE +++R
Sbjct: 20  LIFLQAETTTQISTKKTYVIHMDKSAM-PLPYTNHLQWYSSKINSVTQHKSQEEEGNNNR 79

Query: 73  LLYSYSNAMEGFAAQLTETELEYLKKLPDVVAVREDRKYQIQTTYSHKFLGLS-VGTQGV 132
           +LY+Y  A  G AAQLT+ E E L++   VVAV  + +Y++ TT S  FLGL    ++ V
Sbjct: 80  ILYTYQTAFHGLAAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSPTFLGLERQESERV 139

Query: 133 WQKSSMGQGAIVGLLDTGVWPESPSFSDSKMPPIPQKWRGACQEGQDFNSSNCNRKLIGA 192
           W +       +VG+LDTG+WPES SF+D+ M P+P  WRGAC+ G+ F   NCNRK++GA
Sbjct: 140 WAERVTDHDVVVGVLDTGIWPESESFNDTGMSPVPATWRGACETGKRFLKRNCNRKIVGA 199

Query: 193 KFFIKGHHVASSLPSDVVQEYVSPRDSHGHGTHTSSTAAGASVADASVFGNGAGVAQGMA 252
           + F +G+  A+    D   EY SPRD  GHGTHT++T AG+ V  A++FG   G A+GMA
Sbjct: 200 RVFYRGYEAATG-KIDEELEYKSPRDRDGHGTHTAATVAGSPVKGANLFGFAYGTARGMA 259

Query: 253 PGAHIAVYKVCWFSGCYSSDIVAAMDSAISDGVDILSLSLGGFPLPFFDDSIAIG----- 312
             A +A YKVCW  GC+SSDI++A+D A++DGV +LS+SLGG    +  DS++I      
Sbjct: 260 QKARVAAYKVCWVGGCFSSDILSAVDQAVADGVQVLSISLGGGVSTYSRDSLSIATFGAM 319

Query: 313 -----IVCAAGNNGPTQSSVANVAPWITTIGAGTLDRRFPAIVRLSNGEAIYGESMYPGN 372
                + C+AGN GP   S+ NV+PWITT+GA T+DR FPA V++       G S+Y G 
Sbjct: 320 EMGVFVSCSAGNGGPDPISLTNVSPWITTVGASTMDRDFPATVKIGTMRTFKGVSLYKGR 379

Query: 373 KFKQATKELEVVYLTGGQMGGE---LCLKGSLPREKVQGKMVVCDRGVNGRSEKGQIVKE 432
                 K+  +VYL       +    CL G+L R  V GK+V+CDRGV  R +KGQ+VK 
Sbjct: 380 TVLPKNKQYPLVYLGRNASSPDPTSFCLDGALDRRHVAGKIVICDRGVTPRVQKGQVVKR 439

Query: 433 SGGAAMILANSEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTNPKARIQFGGTVIG 492
           +GG  M+L N+  N EE + D H+LPA  +G  E   +K Y  T+    A ++  GT IG
Sbjct: 440 AGGIGMVLTNTATNGEELVADSHMLPAVAVGEKEGKLIKQYAMTSKKATASLEILGTRIG 499

Query: 493 RSRAPSVAQFSSRGPSLSNPSTLKPDVIAPGVNIIAAWPQNLGPTSLPEDSRRSNFTVMS 552
              +P VA FSSRGP+  +   LKPD++APGVNI+AAW  ++ P+SL  D RR  F ++S
Sbjct: 500 IKPSPVVAAFSSRGPNFLSLEILKPDLLAPGVNILAAWTGDMAPSSLSSDPRRVKFNILS 559

Query: 553 GTSMACPHVSGITALIHSAHPKWTPAAIKSAIMTTADVTDHFGKQILD--GNKPADVFAM 612
           GTSM+CPHVSG+ ALI S HP W+PAAIKSA+MTTA V D+  K + D  G  P+  +  
Sbjct: 560 GTSMSCPHVSGVAALIKSRHPDWSPAAIKSALMTTAYVHDNMFKPLTDASGAAPSSPYDH 619

Query: 613 GAGHVNPTKAIDPGLVYDIKPYEYIIHLCALGYTHSEIFIIT-HMNVSCHKVLQMNKGFT 672
           GAGH++P +A DPGLVYDI P EY   LC    + S++ + T H N +C   L  N G  
Sbjct: 620 GAGHIDPLRATDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFTKHSNRTCKHTLAKNPG-N 679

Query: 673 LNYPSMSVIFKHGTTSKMVS--RRLTNVGSTNSIYEVKVTAPEGVRVRVKPRRLVFKHVN 732
           LNYP++S +F   T  K ++  R +TNVG   S Y+V V+  +G  V V+P+ L F   +
Sbjct: 680 LNYPAISALFPENTHVKAMTLRRTVTNVGPHISSYKVSVSPFKGASVTVQPKTLNFTSKH 739

Query: 733 QSLNYKVWF---IEVYRRDLTWIHCENSKYKVRSPIVVTW 747
           Q L+Y V F     + R +   +  +++ +KVRSP+++TW
Sbjct: 740 QKLSYTVTFRTRFRMKRPEFGGLVWKSTTHKVRSPVIITW 776