IVF0023697 (gene) Melon (IVF77) v1

Overview
NameIVF0023697
Typegene
OrganismCucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
Descriptionsubtilisin-like protease SBT5.3
Locationchr06: 23694108 .. 23699336 (-)
RNA-Seq ExpressionIVF0023697
SyntenyIVF0023697
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
GAAAATAATTACGATCATTTAGAATTGCAAAACAAAAGTTAATGGAATACTTCTCTTATATTGTTTCTCCATTACTATTGTTTTTCTTTTTGCTTCAAACATCTGCCCTTCCTACCAAAAAGGTAAGTATTAGTTTCTTTTCCTCACTACAAGGAAAAAAAAGAAAAAAAAAGCCCTTTTCTCGACTATCGATTCAAAAACTAAAAACGAGAAGAAAAAAGACGAGTTTATGACAATTTTTTAGAGTCGTAAAATATGATGTTGTAGTATACTCATCGATTTTGTGTTTTATGATTTTCTTTTGGGTGTAGTCTTACATTGTTTATTTGGGAGCGCACTCGTTCGGACCGAACCCTACAAACTTTGACATCGAAGCAGCAACCGAGTCACATTATGATCTATTAGGATCAGTAGTGGGAAAGTACAATAATGTCAAATTCATTGCTAAGTTTGATGTATAATGAATTTGATTTTCTGTAGTACTTATTTTATGGGTTGTTGTTTTATTTTTCTTGTCATATGTAGCAATTTGGAAGCCAAAGATTCAATTAAGTATTCTTACAATAAATACATTAATGGTTTTGCTGCTGTTCTTGATGAGCAACAAGCCACGGATCTTGCAAGTAAGAGTTCTTCCTTCTTTTTTTTTTTTTTTTAAATTATTTATTTCTTTTTAAATTTTGAAAAATTAACTGGATTGTTAACTTTTCATTATTGATTCTTAGATTTTAAAAGTAGTGAAAAAAGTTGATACACGTTTGAAGTCGTCTTATTTGATAACCATTTGATTTTTTGAAAATGACCACGTTTAAAAATAATACTATATTTCGTTTGTTGATTTTCTCTTATTTGTTATCTAAATTTTAGTAATTTTAAAAATGCAAACCAATTAAGTTTGAGAACTCTTTTTTTTTTTAATAAATAAAAATAGTTTTCATTTTTGAAATTTGACTAAGAATTCTCCGTTAATAGGAGACAATGAATCCTTAGCTTAGTAAAAGAAGTTGGTAATAAAATAAGCATAAATGTTTTTTAAAAAAAATAAAATGTTCGCCAAACGTGATCTAAAAATTGATTTATTGCTTATTGTTTTATTTTAAAAGTAAAAATAAAGAATAATTTTGTCCTTAGTTCTTCCATATTTAACCATTTATAAATATTAGAAGATTAAGTATAGTTTAATTTTTTTTAGTGTTTCTAAAAAGAAAATGATCAAAAATAGTAAATTTGGCAAAATATTTAAAAGCTATAGAAAAATTTTAGATTTTATCAATAATAAATCTTTGATAGACAATGATAGAAGTCTATTGGTTTCTATCATTGACAGAATCCAAAACTTTTGCTATAATTTGTAATATTTTAATTTATTTTGCTAGTTTTAAAATACCTCTTTTTAAAAATGTCCATTTTTAAAATAGTAAATAATGAATCAATAATAAAAAAAAAAAAAAAGAAGAAAAACAAAATTTTGTTTTTTGTTTTTTTTCTTCCAAAGATATTATTAAAGTTGAGAAACTAAGGTATATAAAATATGTTATCCAACAAATTTTAAAAAATTGTTGATTGTGATCTCTCCTAATTGTGTTTATGGTTAAAGAGATCTTTTCTTCAAATTAAACCTCTCCAAATATTAATATTGCATCTCCTCCCATTTATAAAAAGTTTTGAATATTTAACAAATTTTGATGAACGTTTTCACGAAATGTAATAGAGAATCCAAAGGTGGTGTCGGTATTTGAAAACAAGAAAAGACAATTGCATACAACACGATCATGGAGTTTTCTTGGAATGGAAAATGATGAAGGAATTCCTTCAAACTCCATTTGGAAAGCTGCAAGGTTTGGTGAAGATACAATTATAGGCAACCTTGACTCAGGTCTGTTCTCTTCTTCTTCCTCATTAAACCTCTTCTTTTCTTTATTTTCAACGTCGGACCTTGACGATAATTATAGTAGTTGTAAATGTGACGATTAAGTAATTTATGTGACATTATTTGAATGAAGGTGTTTGGCCAGAGTCGAAGAGCTTCAACGATGCAGGCTATGGTCCTGTTCCTTCAAGGTGGAGGGGAGCTTGTGAAGGTGGCAACAACTTTCAATGCAATAGGTTTCTTTCTCTCCTTTCCTTTCGGGTTAAATTACTTTATATTTTAAAAATATGACGCAATGGTAATGTTGAAAAACAAGCCAAATACAAACTAAAAGCCAAAACAACCTAATGATATTTCTCTCATAAATTTCATTGATCAAAATAAATTTGTGTGAAAAAATACAGGAAGTTGATTGGAGCAAGGTATTTCCACGCAGGATTTGTAATGGGAAGTGGTCTTATCAACGTTAGCTTCAATAAGACAGCAAGAGATGAAGATGGCCATGGTTCACACACTTTATCCACAGCCGGAGGCAACTTTGTCCCCGGAGCCAGTATATTTGGCTATGGCAATGGAACTGCAAAAGGAGGTTCCCCAAAAGCTCGTGTTGCTGCCTACAGGGTTTGCTGGCCTCCGGCTTTATTTGGTGGTGGCTGTTACGATGCTGATATCTTAGCCGGTTTTGAAGCCGCCATTAGTGATGGTGTCGATGTTCTCTCCGTCTCTCTTGGTAGCGAAGCTCAAGAGTTTTCTCATGATTCCATGTCCATAGGGGCATTCCATGCTGTTGAACAAGGAATCGTCGTGGTTTGCTCTGCTGGAAATAGTGGTCCGGGTCCCAAGACTGTAAGCAATGTATCTCCTTGGATGTTCACTGTCGGAGCTAGCACCATCGATCGAGATTTCACAAGTTATGCTACCCTTGGAAACAAGAAGCAATATAAGGTAGATCATACCTCAACCCTTATAGTTCATAAATTTTAATTAGTCTTCATAGTTTTGATCAATTGAAAAATATTTAGGTGTCTCCTAGATATTATAAACCGAGCTCATCTATTTCTAGAATGCGTGCCACATTAGACTTGACATAATCAACTCATTTTGTGAAGGTTGTATATTAACTTTGGTGATATTTAACCAAACCATAATCATCATAAAATATTTATAAACTTTTGTATATGTAGTCATTTGCTTTAGTTAACCTCAAACTTTACATTCTAGGGTTCAAGTCTTTCATCGAGTTCATTGGCTGGTGGTAAATTCTACCCTTTGATAAATGCTGTAGATGCAAGAAATCCTAATTCGACGGACAGCTTTGCGTAAGTCTTACCTCCACTTTTGTGAACAATTTTCAGAAACATGATTCAAATAATCTCTACCAAACACATAGAACAACTAGGAACATATATAAAATAGAGAATTCAGATCTTCCCAAAACAGATTCTTAATTATTCGTGAAGAATAACGTAGATGCATAACTTTGAATTAACATAAACTTTTGACAATTTGTTTGAGAAGGCAAAAATGCGAGACGGGATCGCTTGATCCAGCAAAGGTAAAAGGGAAGATTGTAGTTTGTCTTCGAGGAGTAACTGCAAGAGTTGAGAAAGGTTATGTTGTTCTTCAAGCTGGTGGTGTTGGTATGATTTTAGTGAATGACAAGACCGATGGATCAGGCCTTATTTCTGATTCCCATATACTTCCTGCCACTAGTCTTACCTACACCGATGGACTTGCTGTTGCTCAATATATTAACTCCACCACGTAACATTTCTCATCTATTTTTCTTTCACTTTTGCTTTCCTTTTATATATCTATGTGACAAAATCATCTTAATTAATTTATGCACTTCTCTAAATGAAACCAGAACACCCGTTGCTCTCATAACACCTGTAGAGACTCAGTTAGGAGTTAAACCATCACCTGTCATGGCTGGCTTCTCATCAAGAGGCCCTAATCCCATCACTGACGCCATGCTCAAGGTTGGTTTTTAACTTTTTTAACTCACTAAATGCATGTTTAACATTAAAGTTATTATGTGTTCTAACTCAAATCTTAATATTTCGTATCTAGCCTGATATAACAGGACCGGGAGTGAATATTCTCGCATCGGTCACAACAGATGTAACTGCAACGAAATTTCCATTCGATACGCGTCGGGTGCCTTTCAACGTTGAATCGGGCACTTCCATGTCTTGCCCACACATCGCAGGTGTTGCTGGTCTTCTCAAGACTCTTTATCCAACATGGAGTCCAGCAGCTATCAAATCTGCCATCATGACTACAGGTTCGTTCACATTAAACTTATCCAAAGCTTGAAAAACTATTTAATCTATTTTTGTTCATATAAAGTAATTCAAGAACTGAAATTTTTGTATTTTAAGAATTCAAGTGTCGAAATAGGATAATTATTTGAATTCCTAACCATTTATTAAATTTTCTCTTCGAATTATGTACATGCTAGTTGAGCTATGGTTTATTTTGACTTAAATTTTAAAAGAACGTTTTAGTACGTAAATACCAATGGATTTAGGTATAGGCTCAACCTGCAATGCTCTTTATATAATTTATACATAAAAAACTGAAACAACATCAATACTGGTAAACAATAGTTAGCCCAATATGGTGAAAGCGTTGTCCAATCCCTTCTAAACCCAGGTTTTAGATTCATTCCTTATGTTTTTTTCTATCTTTTTGTGATTGTTTAATATTACAAGCTCAAACTCAAAATTAAAGTAACTAAAAAAGTCTCTCCTCTTTGTTCTTCCCTTTGTCTAATCCATTAAATGACACACATTTGATTTTGTTTTGCAGCCAAAACAAGAGACAACTCCATGCATACATTATCAGACACCGTGAAGGCGAAGGCAACCCCATTTGATTATGGTGCAGGACATGTGAATCCCAATAATGCAATGGACCCCGGCCTCGTTTACGACACAACCATCGACGATTACTTGAACTTCTTATGTGCACGTGGCTACAACGCCCAAACTCTGATGAGTTTCCACAGCAAACCATTTGTGTGCGCAACACCGTTCACATTGACCGATCTCAACTACCCATCAATCTCGATCCCAGAGTTGAAATCCGGTGCCCCAGTAACGGTGAATAGAAGAGTTAAGAATGTGGGAACCCCAGGAACATACGTGGCACGTGTGAAGGCATCCTCAAAGATCTCAGTTACGGTTGAGCCGAGTACGTTGCAGTTTAATAGTGTGGGAGAAGAGAAGGCTTTCAAGGTTGTGTTTGAATACAAAGGAACAGGACAAGGTAAAGGCTATGTGTTTGGGACATTGATATGGTCTGATAATGGGAAGCATAATGTTAGAAGTCCTATTGTAGTGAAATTGGGATGA

mRNA sequence

GAAAATAATTACGATCATTTAGAATTGCAAAACAAAAGTTAATGGAATACTTCTCTTATATTGTTTCTCCATTACTATTGTTTTTCTTTTTGCTTCAAACATCTGCCCTTCCTACCAAAAAGTCTTACATTGTTTATTTGGGAGCGCACTCGTTCGGACCGAACCCTACAAACTTTGACATCGAAGCAGCAACCGAGTCACATTATGATCTATTAGGATCAGTAGTGGGAAACAATTTGGAAGCCAAAGATTCAATTAAGTATTCTTACAATAAATACATTAATGGTTTTGCTGCTGTTCTTGATGAGCAACAAGCCACGGATCTTGCAAAGAATCCAAAGGTGGTGTCGGTATTTGAAAACAAGAAAAGACAATTGCATACAACACGATCATGGAGTTTTCTTGGAATGGAAAATGATGAAGGAATTCCTTCAAACTCCATTTGGAAAGCTGCAAGGTTTGGTGAAGATACAATTATAGGCAACCTTGACTCAGGTGTTTGGCCAGAGTCGAAGAGCTTCAACGATGCAGGCTATGGTCCTGTTCCTTCAAGGTGGAGGGGAGCTTGTGAAGGTGGCAACAACTTTCAATGCAATAGGAAGTTGATTGGAGCAAGGTATTTCCACGCAGGATTTGTAATGGGAAGTGGTCTTATCAACGTTAGCTTCAATAAGACAGCAAGAGATGAAGATGGCCATGGTTCACACACTTTATCCACAGCCGGAGGCAACTTTGTCCCCGGAGCCAGTATATTTGGCTATGGCAATGGAACTGCAAAAGGAGGTTCCCCAAAAGCTCGTGTTGCTGCCTACAGGGTTTGCTGGCCTCCGGCTTTATTTGGTGGTGGCTGTTACGATGCTGATATCTTAGCCGGTTTTGAAGCCGCCATTAGTGATGGTGTCGATGTTCTCTCCGTCTCTCTTGGTAGCGAAGCTCAAGAGTTTTCTCATGATTCCATGTCCATAGGGGCATTCCATGCTGTTGAACAAGGAATCGTCGTGGTTTGCTCTGCTGGAAATAGTGGTCCGGGTCCCAAGACTGTAAGCAATGTATCTCCTTGGATGTTCACTGTCGGAGCTAGCACCATCGATCGAGATTTCACAAGTTATGCTACCCTTGGAAACAAGAAGCAATATAAGGGTTCAAGTCTTTCATCGAGTTCATTGGCTGGTGGTAAATTCTACCCTTTGATAAATGCTGTAGATGCAAGAAATCCTAATTCGACGGACAGCTTTGCGCAAAAATGCGAGACGGGATCGCTTGATCCAGCAAAGGTAAAAGGGAAGATTGTAGTTTGTCTTCGAGGAGTAACTGCAAGAGTTGAGAAAGGTTATGTTGTTCTTCAAGCTGGTGGTGTTGGTATGATTTTAGTGAATGACAAGACCGATGGATCAGGCCTTATTTCTGATTCCCATATACTTCCTGCCACTAGTCTTACCTACACCGATGGACTTGCTGTTGCTCAATATATTAACTCCACCACAACACCCGTTGCTCTCATAACACCTGTAGAGACTCAGTTAGGAGTTAAACCATCACCTGTCATGGCTGGCTTCTCATCAAGAGGCCCTAATCCCATCACTGACGCCATGCTCAAGCCTGATATAACAGGACCGGGAGTGAATATTCTCGCATCGGTCACAACAGATGTAACTGCAACGAAATTTCCATTCGATACGCGTCGGGTGCCTTTCAACGTTGAATCGGGCACTTCCATGTCTTGCCCACACATCGCAGGTGTTGCTGGTCTTCTCAAGACTCTTTATCCAACATGGAGTCCAGCAGCTATCAAATCTGCCATCATGACTACAGCCAAAACAAGAGACAACTCCATGCATACATTATCAGACACCGTGAAGGCGAAGGCAACCCCATTTGATTATGGTGCAGGACATGTGAATCCCAATAATGCAATGGACCCCGGCCTCGTTTACGACACAACCATCGACGATTACTTGAACTTCTTATGTGCACGTGGCTACAACGCCCAAACTCTGATGAGTTTCCACAGCAAACCATTTGTGTGCGCAACACCGTTCACATTGACCGATCTCAACTACCCATCAATCTCGATCCCAGAGTTGAAATCCGGTGCCCCAGTAACGGTGAATAGAAGAGTTAAGAATGTGGGAACCCCAGGAACATACGTGGCACGTGTGAAGGCATCCTCAAAGATCTCAGTTACGGTTGAGCCGAGTACGTTGCAGTTTAATAGTGTGGGAGAAGAGAAGGCTTTCAAGGTTGTGTTTGAATACAAAGGAACAGGACAAGGTAAAGGCTATGTGTTTGGGACATTGATATGGTCTGATAATGGGAAGCATAATGTTAGAAGTCCTATTGTAGTGAAATTGGGATGA

Coding sequence (CDS)

ATGGAATACTTCTCTTATATTGTTTCTCCATTACTATTGTTTTTCTTTTTGCTTCAAACATCTGCCCTTCCTACCAAAAAGTCTTACATTGTTTATTTGGGAGCGCACTCGTTCGGACCGAACCCTACAAACTTTGACATCGAAGCAGCAACCGAGTCACATTATGATCTATTAGGATCAGTAGTGGGAAACAATTTGGAAGCCAAAGATTCAATTAAGTATTCTTACAATAAATACATTAATGGTTTTGCTGCTGTTCTTGATGAGCAACAAGCCACGGATCTTGCAAAGAATCCAAAGGTGGTGTCGGTATTTGAAAACAAGAAAAGACAATTGCATACAACACGATCATGGAGTTTTCTTGGAATGGAAAATGATGAAGGAATTCCTTCAAACTCCATTTGGAAAGCTGCAAGGTTTGGTGAAGATACAATTATAGGCAACCTTGACTCAGGTGTTTGGCCAGAGTCGAAGAGCTTCAACGATGCAGGCTATGGTCCTGTTCCTTCAAGGTGGAGGGGAGCTTGTGAAGGTGGCAACAACTTTCAATGCAATAGGAAGTTGATTGGAGCAAGGTATTTCCACGCAGGATTTGTAATGGGAAGTGGTCTTATCAACGTTAGCTTCAATAAGACAGCAAGAGATGAAGATGGCCATGGTTCACACACTTTATCCACAGCCGGAGGCAACTTTGTCCCCGGAGCCAGTATATTTGGCTATGGCAATGGAACTGCAAAAGGAGGTTCCCCAAAAGCTCGTGTTGCTGCCTACAGGGTTTGCTGGCCTCCGGCTTTATTTGGTGGTGGCTGTTACGATGCTGATATCTTAGCCGGTTTTGAAGCCGCCATTAGTGATGGTGTCGATGTTCTCTCCGTCTCTCTTGGTAGCGAAGCTCAAGAGTTTTCTCATGATTCCATGTCCATAGGGGCATTCCATGCTGTTGAACAAGGAATCGTCGTGGTTTGCTCTGCTGGAAATAGTGGTCCGGGTCCCAAGACTGTAAGCAATGTATCTCCTTGGATGTTCACTGTCGGAGCTAGCACCATCGATCGAGATTTCACAAGTTATGCTACCCTTGGAAACAAGAAGCAATATAAGGGTTCAAGTCTTTCATCGAGTTCATTGGCTGGTGGTAAATTCTACCCTTTGATAAATGCTGTAGATGCAAGAAATCCTAATTCGACGGACAGCTTTGCGCAAAAATGCGAGACGGGATCGCTTGATCCAGCAAAGGTAAAAGGGAAGATTGTAGTTTGTCTTCGAGGAGTAACTGCAAGAGTTGAGAAAGGTTATGTTGTTCTTCAAGCTGGTGGTGTTGGTATGATTTTAGTGAATGACAAGACCGATGGATCAGGCCTTATTTCTGATTCCCATATACTTCCTGCCACTAGTCTTACCTACACCGATGGACTTGCTGTTGCTCAATATATTAACTCCACCACAACACCCGTTGCTCTCATAACACCTGTAGAGACTCAGTTAGGAGTTAAACCATCACCTGTCATGGCTGGCTTCTCATCAAGAGGCCCTAATCCCATCACTGACGCCATGCTCAAGCCTGATATAACAGGACCGGGAGTGAATATTCTCGCATCGGTCACAACAGATGTAACTGCAACGAAATTTCCATTCGATACGCGTCGGGTGCCTTTCAACGTTGAATCGGGCACTTCCATGTCTTGCCCACACATCGCAGGTGTTGCTGGTCTTCTCAAGACTCTTTATCCAACATGGAGTCCAGCAGCTATCAAATCTGCCATCATGACTACAGCCAAAACAAGAGACAACTCCATGCATACATTATCAGACACCGTGAAGGCGAAGGCAACCCCATTTGATTATGGTGCAGGACATGTGAATCCCAATAATGCAATGGACCCCGGCCTCGTTTACGACACAACCATCGACGATTACTTGAACTTCTTATGTGCACGTGGCTACAACGCCCAAACTCTGATGAGTTTCCACAGCAAACCATTTGTGTGCGCAACACCGTTCACATTGACCGATCTCAACTACCCATCAATCTCGATCCCAGAGTTGAAATCCGGTGCCCCAGTAACGGTGAATAGAAGAGTTAAGAATGTGGGAACCCCAGGAACATACGTGGCACGTGTGAAGGCATCCTCAAAGATCTCAGTTACGGTTGAGCCGAGTACGTTGCAGTTTAATAGTGTGGGAGAAGAGAAGGCTTTCAAGGTTGTGTTTGAATACAAAGGAACAGGACAAGGTAAAGGCTATGTGTTTGGGACATTGATATGGTCTGATAATGGGAAGCATAATGTTAGAAGTCCTATTGTAGTGAAATTGGGATGA

Protein sequence

MEYFSYIVSPLLLFFFLLQTSALPTKKSYIVYLGAHSFGPNPTNFDIEAATESHYDLLGSVVGNNLEAKDSIKYSYNKYINGFAAVLDEQQATDLAKNPKVVSVFENKKRQLHTTRSWSFLGMENDEGIPSNSIWKAARFGEDTIIGNLDSGVWPESKSFNDAGYGPVPSRWRGACEGGNNFQCNRKLIGARYFHAGFVMGSGLINVSFNKTARDEDGHGSHTLSTAGGNFVPGASIFGYGNGTAKGGSPKARVAAYRVCWPPALFGGGCYDADILAGFEAAISDGVDVLSVSLGSEAQEFSHDSMSIGAFHAVEQGIVVVCSAGNSGPGPKTVSNVSPWMFTVGASTIDRDFTSYATLGNKKQYKGSSLSSSSLAGGKFYPLINAVDARNPNSTDSFAQKCETGSLDPAKVKGKIVVCLRGVTARVEKGYVVLQAGGVGMILVNDKTDGSGLISDSHILPATSLTYTDGLAVAQYINSTTTPVALITPVETQLGVKPSPVMAGFSSRGPNPITDAMLKPDITGPGVNILASVTTDVTATKFPFDTRRVPFNVESGTSMSCPHIAGVAGLLKTLYPTWSPAAIKSAIMTTAKTRDNSMHTLSDTVKAKATPFDYGAGHVNPNNAMDPGLVYDTTIDDYLNFLCARGYNAQTLMSFHSKPFVCATPFTLTDLNYPSISIPELKSGAPVTVNRRVKNVGTPGTYVARVKASSKISVTVEPSTLQFNSVGEEKAFKVVFEYKGTGQGKGYVFGTLIWSDNGKHNVRSPIVVKLG
Homology
BLAST of IVF0023697 vs. ExPASy Swiss-Prot
Match: Q9ZSP5 (Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=1)

HSP 1 Score: 855.1 bits (2208), Expect = 6.0e-247
Identity = 439/770 (57.01%), Postives = 549/770 (71.30%), Query Frame = 0

Query: 4   FSYIVSPLLLFFFLLQTSALPTKKSYIVYLGAHSFGPNPTNFDIEAATESHYDLLGSVVG 63
           FS+++  LL+        A     SY+VY GAHS     T   ++   E+HYD LGS  G
Sbjct: 7   FSFLLLLLLVHMSSKHILASKDSSSYVVYFGAHSHVGEITEDAMDRVKETHYDFLGSFTG 66

Query: 64  NNLEAKDSIKYSYNKYINGFAAVLDEQQATDLAKNPKVVSVFENKKRQLHTTRSWSFLGM 123
           +   A D+I YSY K+INGFAA LD   A +++K+P+VVSVF NK  +LHTTRSW FLG+
Sbjct: 67  SRERATDAIFYSYTKHINGFAAHLDHDLAYEISKHPEVVSVFPNKALKLHTTRSWDFLGL 126

Query: 124 ENDEGIPSNSIWKAARFGEDTIIGNLDSGVWPESKSFNDAGYGPVPSRWRGACEGGNN-- 183
           E++  +PS+SIW+ ARFGEDTII NLD+GVWPESKSF D G GP+PSRW+G C+   +  
Sbjct: 127 EHNSYVPSSSIWRKARFGEDTIIANLDTGVWPESKSFRDEGLGPIPSRWKGICQNQKDAT 186

Query: 184 FQCNRKLIGARYFHAGFVMGSGLINVSFNKTARDEDGHGSHTLSTAGGNFVPGASIFGYG 243
           F CNRKLIGARYF+ G+    G +N SF+ + RD DGHGSHTLSTA G+FVPG SIFG G
Sbjct: 187 FHCNRKLIGARYFNKGYAAAVGHLNSSFD-SPRDLDGHGSHTLSTAAGDFVPGVSIFGQG 246

Query: 244 NGTAKGGSPKARVAAYRVCWPPALFGGGCYDADILAGFEAAISDGVDVLSVSLGSEAQEF 303
           NGTAKGGSP+ARVAAY+VCWPP + G  CYDAD+LA F+AAI DG DV+SVSLG E   F
Sbjct: 247 NGTAKGGSPRARVAAYKVCWPP-VKGNECYDADVLAAFDAAIHDGADVISVSLGGEPTSF 306

Query: 304 SHDSMSIGAFHAVEQGIVVVCSAGNSGPGPKTVSNVSPWMFTVGASTIDRDFTSYATLGN 363
            +DS++IG+FHA ++ IVVVCSAGNSGP   TVSNV+PW  TVGAST+DR+F S   LGN
Sbjct: 307 FNDSVAIGSFHAAKKRIVVVCSAGNSGPADSTVSNVAPWQITVGASTMDREFASNLVLGN 366

Query: 364 KKQYKGSSLSSSSLAGGKFYPLINAVDARNPNSTDSFAQKCETGSLDPAKVKGKIVVCLR 423
            K YKG SLSS++L   KFYP++ +V+A+  N++   AQ C+ GSLDP K KGKI+VCLR
Sbjct: 367 GKHYKGQSLSSTALPHAKFYPIMASVNAKAKNASALDAQLCKLGSLDPIKTKGKILVCLR 426

Query: 424 GVTARVEKGYVVLQAGGVGMILVNDKTDGSGLISDSHILPATSLTYTDGLAVAQYINSTT 483
           G   RVEKG  V   GG+GM+L N    G+ L++D H+LPAT LT  D  AV++YI+ T 
Sbjct: 427 GQNGRVEKGRAVALGGGIGMVLENTYVTGNDLLADPHVLPATQLTSKDSFAVSRYISQTK 486

Query: 484 TPVALITPVETQLGVKPSPVMAGFSSRGPNPITDAMLKPDITGPGVNILASVTTDVTATK 543
            P+A ITP  T LG+KP+PVMA FSS+GP+ +   +LKPDIT PGV+++A+ T  V+ T 
Sbjct: 487 KPIAHITPSRTDLGLKPAPVMASFSSKGPSIVAPQILKPDITAPGVSVIAAYTGAVSPTN 546

Query: 544 FPFDTRRVPFNVESGTSMSCPHIAGVAGLLKTLYPTWSPAAIKSAIMTTAKTRDNSMHTL 603
             FD RR+ FN  SGTSMSCPHI+G+AGLLKT YP+WSPAAI+SAIMTTA   D+    +
Sbjct: 547 EQFDPRRLLFNAISGTSMSCPHISGIAGLLKTRYPSWSPAAIRSAIMTTATIMDDIPGPI 606

Query: 604 SDTVKAKATPFDYGAGHVNPNNAMDPGLVYDTTIDDYLNFLCARGYNAQTLMSFHSKPFV 663
            +    KATPF +GAGHV PN A++PGLVYD  I DYLNFLC+ GYNA  +  F    F 
Sbjct: 607 QNATNMKATPFSFGAGHVQPNLAVNPGLVYDLGIKDYLNFLCSLGYNASQISVFSGNNFT 666

Query: 664 CATP-FTLTDLNYPSISIPELKSGAPVTVNRRVKNVGTPGTYVARVKASSKISVTVEPST 723
           C++P  +L +LNYPSI++P L S + VTV+R VKNVG P  Y  +V     + V V+P++
Sbjct: 667 CSSPKISLVNLNYPSITVPNLTS-SKVTVSRTVKNVGRPSMYTVKVNNPQGVYVAVKPTS 726

Query: 724 LQFNSVGEEKAFKVVFEYKGTGQGKGYVFGTLIWSDNGKHNVRSPIVVKL 771
           L F  VGE+K FKV+         KGYVFG L+WSD  KH VRSPIVVKL
Sbjct: 727 LNFTKVGEQKTFKVILVKSKGNVAKGYVFGELVWSDK-KHRVRSPIVVKL 772

BLAST of IVF0023697 vs. ExPASy Swiss-Prot
Match: F4JXC5 (Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 SV=1)

HSP 1 Score: 798.5 bits (2061), Expect = 6.6e-230
Identity = 416/764 (54.45%), Postives = 523/764 (68.46%), Query Frame = 0

Query: 8   VSPLLLFFFLLQTSALPTKKSYIVYLGAHSFGPNPTNFDIEAATESHYDLLGSVVGNNLE 67
           +S LLL   L  + A   KKSYIVYLG+H+  P  ++  ++    SH   L S VG++  
Sbjct: 21  LSSLLLLVTLFFSPAFALKKSYIVYLGSHAHLPQISSAHLDGVAHSHRTFLASFVGSHEN 80

Query: 68  AKDSIKYSYNKYINGFAAVLDEQQATDLAKNPKVVSVFENKKRQLHTTRSWSFLGMENDE 127
           AK++I YSY ++INGFAA+LDE +A ++AK+P VVSVF NK R+LHTT SW+F+ +  + 
Sbjct: 81  AKEAIFYSYKRHINGFAAILDENEAAEIAKHPDVVSVFPNKGRKLHTTHSWNFMLLAKNG 140

Query: 128 GIPSNSIWKAARFGEDTIIGNLDSGVWPESKSFNDAGYGPVPSRWRGACEGGNNFQCNRK 187
            +  +S+W  A +GEDTII NLD+GVWPESKSF+D GYG VP+RW+G C    +  CNRK
Sbjct: 141 VVHKSSLWNKAGYGEDTIIANLDTGVWPESKSFSDEGYGAVPARWKGRCH--KDVPCNRK 200

Query: 188 LIGARYFHAGFVMGSGLINVSFNKTARDEDGHGSHTLSTAGGNFVPGASIFGYGNGTAKG 247
           LIGARYF+ G++  +GL + +  +T RD DGHGSHTLSTA GNFVPGA++FG GNGTA G
Sbjct: 201 LIGARYFNKGYLAYTGLPSNASYETCRDHDGHGSHTLSTAAGNFVPGANVFGIGNGTASG 260

Query: 248 GSPKARVAAYRVCWPPALFGGGCYDADILAGFEAAISDGVDVLSVSLGSEAQEFSHDSMS 307
           GSPKARVAAY+VCWPP + G  C+DADILA  EAAI DGVDVLS S+G +A ++  D ++
Sbjct: 261 GSPKARVAAYKVCWPP-VDGAECFDADILAAIEAAIEDGVDVLSASVGGDAGDYMSDGIA 320

Query: 308 IGAFHAVEQGIVVVCSAGNSGPGPKTVSNVSPWMFTVGASTIDRDFTSYATLGNKKQYKG 367
           IG+FHAV+ G+ VVCSAGNSGP   TVSNV+PW+ TVGAS++DR+F ++  L N + +KG
Sbjct: 321 IGSFHAVKNGVTVVCSAGNSGPKSGTVSNVAPWVITVGASSMDREFQAFVELKNGQSFKG 380

Query: 368 SSLSSSSLAGGKFYPLINAVDARNPNSTDSFAQKCETGSLDPAKVKGKIVVCLRGVTARV 427
           +SL S  L   K Y LI+A DA   N   + A  C+ GSLDP KVKGKI+VCLRG  ARV
Sbjct: 381 TSL-SKPLPEEKMYSLISAADANVANGNVTDALLCKKGSLDPKKVKGKILVCLRGDNARV 440

Query: 428 EKGYVVLQAGGVGMILVNDKTDGSGLISDSHILPATSLTYTDGLAVAQYINSTTTPVALI 487
           +KG     AG  GM+L NDK  G+ +ISD+H+LPA+ + Y DG  +  Y++ST  P   I
Sbjct: 441 DKGMQAAAAGAAGMVLCNDKASGNEIISDAHVLPASQIDYKDGETLFSYLSSTKDPKGYI 500

Query: 488 TPVETQLGVKPSPVMAGFSSRGPNPITDAMLKPDITGPGVNILASVTTDVTATKFPFDTR 547
                 L  KP+P MA FSSRGPN IT  +LKPDIT PGVNI+A+ T     T    D R
Sbjct: 501 KAPTATLNTKPAPFMASFSSRGPNTITPGILKPDITAPGVNIIAAFTEATGPTDLDSDNR 560

Query: 548 RVPFNVESGTSMSCPHIAGVAGLLKTLYPTWSPAAIKSAIMTTAKTRDNSMHTLSDTVKA 607
           R PFN ESGTSMSCPHI+GV GLLKTL+P WSPAAI+SAIMTT++TR+N    + D    
Sbjct: 561 RTPFNTESGTSMSCPHISGVVGLLKTLHPHWSPAAIRSAIMTTSRTRNNRRKPMVDESFK 620

Query: 608 KATPFDYGAGHVNPNNAMDPGLVYDTTIDDYLNFLCARGYNAQTLMSFHSKP-FVCATPF 667
           KA PF YG+GHV PN A  PGLVYD T  DYL+FLCA GYN   +  F   P + C    
Sbjct: 621 KANPFSYGSGHVQPNKAAHPGLVYDLTTGDYLDFLCAVGYNNTVVQLFAEDPQYTCRQGA 680

Query: 668 TLTDLNYPSISIPELKSGAPVTVNRRVKNVGTPGTYVARVKASSKISVTVEPSTLQFNSV 727
            L D NYPSI++P L     +TV R++KNVG P TY AR +    + V+VEP  L FN  
Sbjct: 681 NLLDFNYPSITVPNLTGS--ITVTRKLKNVGPPATYNARFREPLGVRVSVEPKQLTFNKT 740

Query: 728 GEEKAFKVVFEYKGTGQGKGYVFGTLIWSDNGKHNVRSPIVVKL 771
           GE K F++           GYVFG L W+D+  H VRSPIVV+L
Sbjct: 741 GEVKIFQMTLRPLPV-TPSGYVFGELTWTDS-HHYVRSPIVVQL 776

BLAST of IVF0023697 vs. ExPASy Swiss-Prot
Match: I1N462 (Subtilisin-like protease Glyma18g48580 OS=Glycine max OX=3847 GN=Glyma18g48580 PE=1 SV=3)

HSP 1 Score: 709.5 bits (1830), Expect = 4.1e-203
Identity = 396/790 (50.13%), Postives = 521/790 (65.95%), Query Frame = 0

Query: 7   IVSPLLLFFFLLQTSALPTKKSYIVYLGAHSFGPNPTNFDIEAATESHYDLLGSVVGNNL 66
           I+S   LF FLL  +   +KK YIVY+GAHS GP+PT+ D+E AT+SHYDLLGS+ G+  
Sbjct: 11  ILSSFFLFTFLL-AAVNGSKKCYIVYMGAHSHGPSPTSADLELATDSHYDLLGSIFGSRE 70

Query: 67  EAKDSIKYSYNKYINGFAAVLDEQQATDLAKNPKVVSVFENKKRQLHTTRSWSFLGMEND 126
           +AK++I YSYN++INGFAA+L+E++A D+AKNP VVSVF +K+ +LHTTRSW FLG+   
Sbjct: 71  KAKEAIIYSYNRHINGFAALLEEEEAADIAKNPNVVSVFLSKEHKLHTTRSWEFLGLHRR 130

Query: 127 EGIPSNSIWKAARFGEDTIIGNLDSGVWPESKSFNDAGYGPVPSRWRGA-CE-----GGN 186
                NS W+  RFGE+TIIGN+D+GVWPES+SF+D GYG VPS+WRG  C+     G  
Sbjct: 131 ---GQNSAWQKGRFGENTIIGNIDTGVWPESQSFSDKGYGTVPSKWRGGLCQINKLPGSM 190

Query: 187 NFQCNRKLIGARYFHAGFVMGSGLINVSFNKTARDEDGHGSHTLSTAGGNFVPGASIFGY 246
              CNRKLIGARY++  F   +G ++   + TARD  GHG+HTLSTAGGNFVPGA +F  
Sbjct: 191 KNTCNRKLIGARYYNKAFEAHNGQLDPLLH-TARDFVGHGTHTLSTAGGNFVPGARVFAV 250

Query: 247 GNGTAKGGSPKARVAAYRVCW---PPALFGGGCYDADILAGFEAAISDGVDVLSVSLGSE 306
           GNGTAKGGSP+ARVAAY+VCW    PA     CY AD+LA  + AI DGVDV++VS G  
Sbjct: 251 GNGTAKGGSPRARVAAYKVCWSLTDPA----SCYGADVLAAIDQAIDDGVDVINVSFGVS 310

Query: 307 ----AQEFSHDSMSIGAFHAVEQGIVVVCSAGNSGPGPKTVSNVSPWMFTVGASTIDRDF 366
               A+    D +SIGAFHA+ + I++V SAGN GP P TV+NV+PW+FT+ AST+DRDF
Sbjct: 311 YVVTAEGIFTDEISIGAFHAISKNILLVASAGNDGPTPGTVANVAPWVFTIAASTLDRDF 370

Query: 367 TSYATLGNKKQYKGSSLSSSSLAGGKFYPLINAVDARNPNSTDSFAQKCETGSLDPAKVK 426
           +S  T+ N +  +G+SL   +L   + + LI + DA+  N+T   AQ C  G+LD  KV 
Sbjct: 371 SSNLTI-NNQLIEGASL-FVNLPPNQAFSLILSTDAKLANATFRDAQLCRRGTLDRTKVN 430

Query: 427 GKIVVCLR-GVTARVEKGYVVLQAGGVGMILVNDKTDGSGLISDSHILPATSLTYTDGLA 486
           GKIV+C R G    V +G   L AG  GMIL N   +G  L ++ H+    +       +
Sbjct: 431 GKIVLCTREGKIKSVAEGLEALTAGARGMILNNQMQNGKTLSAEPHVFSTVNTPPRRAKS 490

Query: 487 VAQYINST----------TTPVALITPVETQLGVKPSPVMAGFSSRGPNPITDAMLKPDI 546
               + +T          T     ++   T  G KP+PVMA FSSRGPN I  ++LKPD+
Sbjct: 491 RPHGVKTTAIGDEDDPLKTGDTIKMSRARTLFGRKPAPVMASFSSRGPNKIQPSILKPDV 550

Query: 547 TGPGVNILASVTTDVTATKFPFDTRR-VPFNVESGTSMSCPHIAGVAGLLKTLYPTWSPA 606
           T PGVNILA+ +   +A+    D RR   FNV  GTSMSCPH +G+AGLLKT +P+WSPA
Sbjct: 551 TAPGVNILAAYSEFASASSLLVDNRRGFKFNVLQGTSMSCPHASGIAGLLKTRHPSWSPA 610

Query: 607 AIKSAIMTTAKTRDNSMHTLSDTV-KAKATPFDYGAGHVNPNNAMDPGLVYDTTIDDYLN 666
           AIKSAIMTTA T DN+   + D   K  A  F YG+GHV P+ A++PGLVYD ++ DYLN
Sbjct: 611 AIKSAIMTTATTLDNTNRPIQDAFDKTLADAFAYGSGHVRPDLAIEPGLVYDLSLTDYLN 670

Query: 667 FLCARGYNAQTLMSFH-SKPFVCATPFTLTDLNYPSISIPELKSGAPVTVNRRVKNVGTP 726
           FLCA GY+ Q + + + ++ F+C+   ++ DLNYPSI++P L+   PVT+ R V NVG P
Sbjct: 671 FLCASGYDQQLISALNFNRTFICSGSHSVNDLNYPSITLPNLRL-KPVTIARTVTNVGPP 730

Query: 727 GTYVARVKASSKISVTVEPSTLQFNSVGEEKAFKVVFEYKGTGQGKGYVFGTLIWSDNGK 770
            TY    ++ +  S+ V P +L F  +GE K FKV+ +       + Y FG L W+D GK
Sbjct: 731 STYTVSTRSPNGYSIAVVPPSLTFTKIGERKTFKVIVQASSAATRRKYEFGDLRWTD-GK 787

BLAST of IVF0023697 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 608.6 bits (1568), Expect = 9.8e-173
Identity = 340/766 (44.39%), Postives = 481/766 (62.79%), Query Frame = 0

Query: 11  LLLFFFLLQTSALPTKKSYIVYLGAHSFGPNPTNFDIEAATESHYDLLGSVVGNNLEAKD 70
           LL   F   +S+   + +YIV++        P++FD+ +   + YD     + ++ E   
Sbjct: 14  LLCLGFCHVSSSSSDQGTYIVHMAKSQM---PSSFDLHS---NWYDSSLRSISDSAE--- 73

Query: 71  SIKYSYNKYINGFAAVLDEQQATDLAKNPKVVSVFENKKRQLHTTRSWSFLGMENDEGIP 130
            + Y+Y   I+GF+  L +++A  L   P V+SV    + +LHTTR+  FLG++      
Sbjct: 74  -LLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEH---- 133

Query: 131 SNSIWKAARFGEDTIIGNLDSGVWPESKSFNDAGYGPVPSRWRGACEGGNNFQ---CNRK 190
           +  ++  A    D ++G LD+GVWPESKS++D G+GP+PS W+G CE G NF    CNRK
Sbjct: 134 TADLFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRK 193

Query: 191 LIGARYFHAGFVMGSGLINVS-FNKTARDEDGHGSHTLSTAGGNFVPGASIFGYGNGTAK 250
           LIGAR+F  G+    G I+ S  +++ RD+DGHG+HT STA G+ V GAS+ GY +GTA+
Sbjct: 194 LIGARFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTAR 253

Query: 251 GGSPKARVAAYRVCWPPALFGGGCYDADILAGFEAAISDGVDVLSVSLGSEAQEFSHDSM 310
           G +P+ARVA Y+VCW      GGC+ +DILA  + AI+D V+VLS+SLG    ++  D +
Sbjct: 254 GMAPRARVAVYKVCWL-----GGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGV 313

Query: 311 SIGAFHAVEQGIVVVCSAGNSGPGPKTVSNVSPWMFTVGASTIDRDFTSYATLGNKKQYK 370
           +IGAF A+E+GI+V CSAGN+GP   ++SNV+PW+ TVGA T+DRDF + A LGN K + 
Sbjct: 314 AIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFT 373

Query: 371 GSSLSSSSLAGGKFYPLINAVDARNPNSTDSFAQKCETGSLDPAKVKGKIVVCLRGVTAR 430
           G SL        K  P I A +A N  +       C TG+L P KVKGKIV+C RG+ AR
Sbjct: 374 GVSLFKGEALPDKLLPFIYAGNASNATN----GNLCMTGTLIPEKVKGKIVMCDRGINAR 433

Query: 431 VEKGYVVLQAGGVGMILVNDKTDGSGLISDSHILPATSLTYTDGLAVAQYINSTTTPVAL 490
           V+KG VV  AGGVGMIL N   +G  L++D+H+LPAT++    G  +  Y+ +   P A 
Sbjct: 434 VQKGDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTAS 493

Query: 491 ITPVETQLGVKPSPVMAGFSSRGPNPITDAMLKPDITGPGVNILASVTTDVTATKFPFDT 550
           I+ + T +GVKPSPV+A FSSRGPN IT  +LKPD+  PGVNILA+ T     T    D+
Sbjct: 494 ISILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDS 553

Query: 551 RRVPFNVESGTSMSCPHIAGVAGLLKTLYPTWSPAAIKSAIMTTA-KTRDNSMHTLSDTV 610
           RRV FN+ SGTSMSCPH++G+A LLK+++P WSPAAI+SA+MTTA KT  +    L    
Sbjct: 554 RRVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIAT 613

Query: 611 KAKATPFDYGAGHVNPNNAMDPGLVYDTTIDDYLNFLCARGYNAQTLMSFHSKPFVC--A 670
              +TPFD+GAGHV+P  A +PGL+YD T +DYL FLCA  Y +  + S   + + C  +
Sbjct: 614 GKPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPS 673

Query: 671 TPFTLTDLNYPSISIPELKSGAPVTVNRRVKNVGTPGTYVARVKA-SSKISVTVEPSTLQ 730
             +++ DLNYPS ++     GA     R V +VG  GTY  +V + ++ + ++VEP+ L 
Sbjct: 674 KSYSVADLNYPSFAVNVDGVGA-YKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLN 733

Query: 731 FNSVGEEKAFKVVFEYKGTGQGKGYVFGTLIWSDNGKHNVRSPIVV 769
           F    E+K++ V F    +       FG++ WSD GKH V SP+ +
Sbjct: 734 FKEANEKKSYTVTFTVDSSKPSGSNSFGSIEWSD-GKHVVGSPVAI 754

BLAST of IVF0023697 vs. ExPASy Swiss-Prot
Match: Q9FLI4 (Subtilisin-like protease SBT1.3 OS=Arabidopsis thaliana OX=3702 GN=SBT1.3 PE=2 SV=1)

HSP 1 Score: 576.2 bits (1484), Expect = 5.4e-163
Identity = 336/786 (42.75%), Postives = 476/786 (60.56%), Query Frame = 0

Query: 4   FSYIVSPLLLFFFLLQTSA-LPTKKSYIVYLGAHSFGPNPTNFDIEAATESHYDLLGSVV 63
           F +I+  + L F   +T+  + TKK+Y++++   +     TN       + +   + SV 
Sbjct: 11  FLFIILSINLIFLQAETTTQISTKKTYVIHMDKSAMPLPYTNH-----LQWYSSKINSVT 70

Query: 64  GNNLEAKDS----IKYSYNKYINGFAAVLDEQQATDLAKNPKVVSVFENKKRQLHTTRSW 123
            +  + ++     I Y+Y    +G AA L +++A  L +   VV+V    + +LHTTRS 
Sbjct: 71  QHKSQEEEGNNNRILYTYQTAFHGLAAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSP 130

Query: 124 SFLGMENDEGIPSNSIWKAARFGEDTIIGNLDSGVWPESKSFNDAGYGPVPSRWRGACEG 183
           +FLG+E  E   S  +W       D ++G LD+G+WPES+SFND G  PVP+ WRGACE 
Sbjct: 131 TFLGLERQE---SERVWAERVTDHDVVVGVLDTGIWPESESFNDTGMSPVPATWRGACET 190

Query: 184 GNNF---QCNRKLIGARYFHAGFVMGSGLINVSFN-KTARDEDGHGSHTLSTAGGNFVPG 243
           G  F    CNRK++GAR F+ G+   +G I+     K+ RD DGHG+HT +T  G+ V G
Sbjct: 191 GKRFLKRNCNRKIVGARVFYRGYEAATGKIDEELEYKSPRDRDGHGTHTAATVAGSPVKG 250

Query: 244 ASIFGYGNGTAKGGSPKARVAAYRVCWPPALFGGGCYDADILAGFEAAISDGVDVLSVSL 303
           A++FG+  GTA+G + KARVAAY+VCW      GGC+ +DIL+  + A++DGV VLS+SL
Sbjct: 251 ANLFGFAYGTARGMAQKARVAAYKVCWV-----GGCFSSDILSAVDQAVADGVQVLSISL 310

Query: 304 GSEAQEFSHDSMSIGAFHAVEQGIVVVCSAGNSGPGPKTVSNVSPWMFTVGASTIDRDFT 363
           G     +S DS+SI  F A+E G+ V CSAGN GP P +++NVSPW+ TVGAST+DRDF 
Sbjct: 311 GGGVSTYSRDSLSIATFGAMEMGVFVSCSAGNGGPDPISLTNVSPWITTVGASTMDRDFP 370

Query: 364 SYATLGNKKQYKGSSL--SSSSLAGGKFYPLINAVDARNPNSTDSFAQKCETGSLDPAKV 423
           +   +G  + +KG SL    + L   K YPL+     RN +S D     C  G+LD   V
Sbjct: 371 ATVKIGTMRTFKGVSLYKGRTVLPKNKQYPLVYL--GRNASSPDP-TSFCLDGALDRRHV 430

Query: 424 KGKIVVCLRGVTARVEKGYVVLQAGGVGMILVNDKTDGSGLISDSHILPATSLTYTDGLA 483
            GKIV+C RGVT RV+KG VV +AGG+GM+L N  T+G  L++DSH+LPA ++   +G  
Sbjct: 431 AGKIVICDRGVTPRVQKGQVVKRAGGIGMVLTNTATNGEELVADSHMLPAVAVGEKEGKL 490

Query: 484 VAQYINSTTTPVALITPVETQLGVKPSPVMAGFSSRGPNPITDAMLKPDITGPGVNILAS 543
           + QY  ++    A +  + T++G+KPSPV+A FSSRGPN ++  +LKPD+  PGVNILA+
Sbjct: 491 IKQYAMTSKKATASLEILGTRIGIKPSPVVAAFSSRGPNFLSLEILKPDLLAPGVNILAA 550

Query: 544 VTTDVTATKFPFDTRRVPFNVESGTSMSCPHIAGVAGLLKTLYPTWSPAAIKSAIMTTAK 603
            T D+  +    D RRV FN+ SGTSMSCPH++GVA L+K+ +P WSPAAIKSA+MTTA 
Sbjct: 551 WTGDMAPSSLSSDPRRVKFNILSGTSMSCPHVSGVAALIKSRHPDWSPAAIKSALMTTAY 610

Query: 604 TRDNSMHTLSD-TVKAKATPFDYGAGHVNPNNAMDPGLVYDTTIDDYLNFLCARGYNAQT 663
             DN    L+D +  A ++P+D+GAGH++P  A DPGLVYD    +Y  FLC +  +   
Sbjct: 611 VHDNMFKPLTDASGAAPSSPYDHGAGHIDPLRATDPGLVYDIGPQEYFEFLCTQDLSPSQ 670

Query: 664 LMSF--HS----KPFVCATPFTLTDLNYPSIS--IPELKSGAPVTVNRRVKNVGTP-GTY 723
           L  F  HS    K  +   P    +LNYP+IS   PE      +T+ R V NVG    +Y
Sbjct: 671 LKVFTKHSNRTCKHTLAKNP---GNLNYPAISALFPENTHVKAMTLRRTVTNVGPHISSY 730

Query: 724 VARVKASSKISVTVEPSTLQFNSVGEEKAFKVVFEYKGTGQGKGYVFGTLIWSDNGKHNV 769
              V      SVTV+P TL F S  ++ ++ V F  +   + K   FG L+W     H V
Sbjct: 731 KVSVSPFKGASVTVQPKTLNFTSKHQKLSYTVTFRTR--FRMKRPEFGGLVWKST-THKV 774

BLAST of IVF0023697 vs. ExPASy TrEMBL
Match: A0A1S3BLG8 (subtilisin-like protease SBT5.3 OS=Cucumis melo OX=3656 GN=LOC103491134 PE=3 SV=1)

HSP 1 Score: 1536.5 bits (3977), Expect = 0.0e+00
Identity = 768/771 (99.61%), Postives = 770/771 (99.87%), Query Frame = 0

Query: 1   MEYFSYIVSPLLLFFFLLQTSALPTKKSYIVYLGAHSFGPNPTNFDIEAATESHYDLLGS 60
           MEYFSYIVSPLLLFFFLLQTSALPTKKSYIVYLGAHSFGPNPTNFDIEAATESHYDLLGS
Sbjct: 1   MEYFSYIVSPLLLFFFLLQTSALPTKKSYIVYLGAHSFGPNPTNFDIEAATESHYDLLGS 60

Query: 61  VVGNNLEAKDSIKYSYNKYINGFAAVLDEQQATDLAKNPKVVSVFENKKRQLHTTRSWSF 120
           VVGNNLEAKDSIKYSYNKYINGFAAVLDEQQATDLAKNPKVVSVFENKKRQLHTTRSWSF
Sbjct: 61  VVGNNLEAKDSIKYSYNKYINGFAAVLDEQQATDLAKNPKVVSVFENKKRQLHTTRSWSF 120

Query: 121 LGMENDEGIPSNSIWKAARFGEDTIIGNLDSGVWPESKSFNDAGYGPVPSRWRGACEGGN 180
           LGMENDEGIPSNSIWKAARFGEDTIIGNLDSGVWPESKSFNDAGYGPVPSRWRGACEGGN
Sbjct: 121 LGMENDEGIPSNSIWKAARFGEDTIIGNLDSGVWPESKSFNDAGYGPVPSRWRGACEGGN 180

Query: 181 NFQCNRKLIGARYFHAGFVMGSGLINVSFNKTARDEDGHGSHTLSTAGGNFVPGASIFGY 240
           NFQCNRKLIGARYFHAGFVMGSGLINVSFNKTARDEDGHGSHTLSTAGGNFVPGASIFGY
Sbjct: 181 NFQCNRKLIGARYFHAGFVMGSGLINVSFNKTARDEDGHGSHTLSTAGGNFVPGASIFGY 240

Query: 241 GNGTAKGGSPKARVAAYRVCWPPALFGGGCYDADILAGFEAAISDGVDVLSVSLGSEAQE 300
           GNGTAKGGSPKARVAAYRVCWPPALFGGGCYDADILAGFEAAISDGVDVLSVSLGSEAQE
Sbjct: 241 GNGTAKGGSPKARVAAYRVCWPPALFGGGCYDADILAGFEAAISDGVDVLSVSLGSEAQE 300

Query: 301 FSHDSMSIGAFHAVEQGIVVVCSAGNSGPGPKTVSNVSPWMFTVGASTIDRDFTSYATLG 360
           FSHDSMSIGAFHAVEQGIVVVCSAGNSGPGPKTVSNVSPWMFTVGASTIDRDFTSYATLG
Sbjct: 301 FSHDSMSIGAFHAVEQGIVVVCSAGNSGPGPKTVSNVSPWMFTVGASTIDRDFTSYATLG 360

Query: 361 NKKQYKGSSLSSSSLAGGKFYPLINAVDARNPNSTDSFAQKCETGSLDPAKVKGKIVVCL 420
           NKKQYKGSSLSSSSLAGGKFYPLINAVDARNPNSTDSFAQKCETGSLDPAKVKGKIVVCL
Sbjct: 361 NKKQYKGSSLSSSSLAGGKFYPLINAVDARNPNSTDSFAQKCETGSLDPAKVKGKIVVCL 420

Query: 421 RGVTARVEKGYVVLQAGGVGMILVNDKTDGSGLISDSHILPATSLTYTDGLAVAQYINST 480
           RGVTARVEKGYVVLQAGGVGMILVNDKTDGSGLISDSHILPATSLTYTDGLAVAQYINST
Sbjct: 421 RGVTARVEKGYVVLQAGGVGMILVNDKTDGSGLISDSHILPATSLTYTDGLAVAQYINST 480

Query: 481 TTPVALITPVETQLGVKPSPVMAGFSSRGPNPITDAMLKPDITGPGVNILASVTTDVTAT 540
           TTPVALITPVETQLGVKPSPVMAGFSSRGPNPITDAMLKPDITGPGVNILASVTTDVTAT
Sbjct: 481 TTPVALITPVETQLGVKPSPVMAGFSSRGPNPITDAMLKPDITGPGVNILASVTTDVTAT 540

Query: 541 KFPFDTRRVPFNVESGTSMSCPHIAGVAGLLKTLYPTWSPAAIKSAIMTTAKTRDNSMHT 600
           KFPFDTRRVPFNVESGTSMSCPHIAGV+GLLKTLYPTWSPAAIKSAIMTTAKTRDNSMHT
Sbjct: 541 KFPFDTRRVPFNVESGTSMSCPHIAGVSGLLKTLYPTWSPAAIKSAIMTTAKTRDNSMHT 600

Query: 601 LSDTVKAKATPFDYGAGHVNPNNAMDPGLVYDTTIDDYLNFLCARGYNAQTLMSFHSKPF 660
           LSDTVKAKATPFDYGAGHVNPNNAMDPGLVYDTTIDDYLNFLCARGYNAQTLMSFHSKPF
Sbjct: 601 LSDTVKAKATPFDYGAGHVNPNNAMDPGLVYDTTIDDYLNFLCARGYNAQTLMSFHSKPF 660

Query: 661 VCATPFTLTDLNYPSISIPELKSGAPVTVNRRVKNVGTPGTYVARVKASSKISVTVEPST 720
           VCATPFTLTDLNYPSISIPELKSGAPVTVNRRVKNVGTPGTYVARVKASSKISVTVEPST
Sbjct: 661 VCATPFTLTDLNYPSISIPELKSGAPVTVNRRVKNVGTPGTYVARVKASSKISVTVEPST 720

Query: 721 LQFNSVGEEKAFKVVFEYKGTGQGKGYVFGTLIWSDNGKHNVRSPIVVKLG 772
           LQFNSVGEEKAFKV+FEYKGTGQGK YVFGTLIWSDNGKHNVRSPIVVKLG
Sbjct: 721 LQFNSVGEEKAFKVLFEYKGTGQGKSYVFGTLIWSDNGKHNVRSPIVVKLG 771

BLAST of IVF0023697 vs. ExPASy TrEMBL
Match: A0A5D3D732 (Subtilisin-like protease SBT5.3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold443G00170 PE=3 SV=1)

HSP 1 Score: 1291.9 bits (3342), Expect = 0.0e+00
Identity = 644/644 (100.00%), Postives = 644/644 (100.00%), Query Frame = 0

Query: 123 MENDEGIPSNSIWKAARFGEDTIIGNLDSGVWPESKSFNDAGYGPVPSRWRGACEGGNNF 182
           MENDEGIPSNSIWKAARFGEDTIIGNLDSGVWPESKSFNDAGYGPVPSRWRGACEGGNNF
Sbjct: 1   MENDEGIPSNSIWKAARFGEDTIIGNLDSGVWPESKSFNDAGYGPVPSRWRGACEGGNNF 60

Query: 183 QCNRKLIGARYFHAGFVMGSGLINVSFNKTARDEDGHGSHTLSTAGGNFVPGASIFGYGN 242
           QCNRKLIGARYFHAGFVMGSGLINVSFNKTARDEDGHGSHTLSTAGGNFVPGASIFGYGN
Sbjct: 61  QCNRKLIGARYFHAGFVMGSGLINVSFNKTARDEDGHGSHTLSTAGGNFVPGASIFGYGN 120

Query: 243 GTAKGGSPKARVAAYRVCWPPALFGGGCYDADILAGFEAAISDGVDVLSVSLGSEAQEFS 302
           GTAKGGSPKARVAAYRVCWPPALFGGGCYDADILAGFEAAISDGVDVLSVSLGSEAQEFS
Sbjct: 121 GTAKGGSPKARVAAYRVCWPPALFGGGCYDADILAGFEAAISDGVDVLSVSLGSEAQEFS 180

Query: 303 HDSMSIGAFHAVEQGIVVVCSAGNSGPGPKTVSNVSPWMFTVGASTIDRDFTSYATLGNK 362
           HDSMSIGAFHAVEQGIVVVCSAGNSGPGPKTVSNVSPWMFTVGASTIDRDFTSYATLGNK
Sbjct: 181 HDSMSIGAFHAVEQGIVVVCSAGNSGPGPKTVSNVSPWMFTVGASTIDRDFTSYATLGNK 240

Query: 363 KQYKGSSLSSSSLAGGKFYPLINAVDARNPNSTDSFAQKCETGSLDPAKVKGKIVVCLRG 422
           KQYKGSSLSSSSLAGGKFYPLINAVDARNPNSTDSFAQKCETGSLDPAKVKGKIVVCLRG
Sbjct: 241 KQYKGSSLSSSSLAGGKFYPLINAVDARNPNSTDSFAQKCETGSLDPAKVKGKIVVCLRG 300

Query: 423 VTARVEKGYVVLQAGGVGMILVNDKTDGSGLISDSHILPATSLTYTDGLAVAQYINSTTT 482
           VTARVEKGYVVLQAGGVGMILVNDKTDGSGLISDSHILPATSLTYTDGLAVAQYINSTTT
Sbjct: 301 VTARVEKGYVVLQAGGVGMILVNDKTDGSGLISDSHILPATSLTYTDGLAVAQYINSTTT 360

Query: 483 PVALITPVETQLGVKPSPVMAGFSSRGPNPITDAMLKPDITGPGVNILASVTTDVTATKF 542
           PVALITPVETQLGVKPSPVMAGFSSRGPNPITDAMLKPDITGPGVNILASVTTDVTATKF
Sbjct: 361 PVALITPVETQLGVKPSPVMAGFSSRGPNPITDAMLKPDITGPGVNILASVTTDVTATKF 420

Query: 543 PFDTRRVPFNVESGTSMSCPHIAGVAGLLKTLYPTWSPAAIKSAIMTTAKTRDNSMHTLS 602
           PFDTRRVPFNVESGTSMSCPHIAGVAGLLKTLYPTWSPAAIKSAIMTTAKTRDNSMHTLS
Sbjct: 421 PFDTRRVPFNVESGTSMSCPHIAGVAGLLKTLYPTWSPAAIKSAIMTTAKTRDNSMHTLS 480

Query: 603 DTVKAKATPFDYGAGHVNPNNAMDPGLVYDTTIDDYLNFLCARGYNAQTLMSFHSKPFVC 662
           DTVKAKATPFDYGAGHVNPNNAMDPGLVYDTTIDDYLNFLCARGYNAQTLMSFHSKPFVC
Sbjct: 481 DTVKAKATPFDYGAGHVNPNNAMDPGLVYDTTIDDYLNFLCARGYNAQTLMSFHSKPFVC 540

Query: 663 ATPFTLTDLNYPSISIPELKSGAPVTVNRRVKNVGTPGTYVARVKASSKISVTVEPSTLQ 722
           ATPFTLTDLNYPSISIPELKSGAPVTVNRRVKNVGTPGTYVARVKASSKISVTVEPSTLQ
Sbjct: 541 ATPFTLTDLNYPSISIPELKSGAPVTVNRRVKNVGTPGTYVARVKASSKISVTVEPSTLQ 600

Query: 723 FNSVGEEKAFKVVFEYKGTGQGKGYVFGTLIWSDNGKHNVRSPI 767
           FNSVGEEKAFKVVFEYKGTGQGKGYVFGTLIWSDNGKHNVRSPI
Sbjct: 601 FNSVGEEKAFKVVFEYKGTGQGKGYVFGTLIWSDNGKHNVRSPI 644

BLAST of IVF0023697 vs. ExPASy TrEMBL
Match: A0A0A0L601 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G045060 PE=3 SV=1)

HSP 1 Score: 1260.4 bits (3260), Expect = 0.0e+00
Identity = 627/744 (84.27%), Postives = 679/744 (91.26%), Query Frame = 0

Query: 28  SYIVYLGAHSFGPNPTNFDIEAATESHYDLLGSVVGNNLEAKDSIKYSYNKYINGFAAVL 87
           SYIVYLGA S G NPTN+DIE ATES YDLLGSVVG+ L AKD+IKYSYNKYINGFAA L
Sbjct: 6   SYIVYLGAPSVGSNPTNYDIEVATESQYDLLGSVVGSKLAAKDAIKYSYNKYINGFAATL 65

Query: 88  DEQQATDLAKNPKVVSVFENKKRQLHTTRSWSFLGMENDEGIPSNSIWKAARFGEDTIIG 147
           DE+QA DLAKNPKVVSVFENK+R+LHTTRSW FLG+E+DEGIPSNSIW A RFGEDTIIG
Sbjct: 66  DEKQAKDLAKNPKVVSVFENKERKLHTTRSWHFLGVESDEGIPSNSIWNAGRFGEDTIIG 125

Query: 148 NLDSGVWPESKSFNDAGYGPVPSRWRGACEGGNNFQCNRKLIGARYFHAGFVMGSGLINV 207
           NLD+GVWPESKSFNDAGYGPVPSRWRGACEGG NF+CNRKLIGARYF+ GF M SG +N+
Sbjct: 126 NLDTGVWPESKSFNDAGYGPVPSRWRGACEGGANFRCNRKLIGARYFNKGFAMASGPLNI 185

Query: 208 SFNKTARDEDGHGSHTLSTAGGNFVPGASIFGYGNGTAKGGSPKARVAAYRVCWPPALFG 267
           SFN TARD+ GHGSHTLSTAGGNFVPGA++FGYGNGTAKGGSPKARVAAY+VCW PA  G
Sbjct: 186 SFN-TARDKQGHGSHTLSTAGGNFVPGANVFGYGNGTAKGGSPKARVAAYKVCW-PATSG 245

Query: 268 GGCYDADILAGFEAAISDGVDVLSVSLGSEAQEFSHDSMSIGAFHAVEQGIVVVCSAGNS 327
           GGCYDADILAGFEAAISDGVDVLSVSLGS+ +EF++DSMSIGAFHAV+QGIVVVCSAGN 
Sbjct: 246 GGCYDADILAGFEAAISDGVDVLSVSLGSKPEEFAYDSMSIGAFHAVQQGIVVVCSAGND 305

Query: 328 GPGPKTVSNVSPWMFTVGASTIDRDFTSYATLGNKKQYKGSSLSSSSLAGGKFYPLINAV 387
           GPGP TVSN+SPWMFTV AS+IDRDFTSYA+LGNKK YKGSS+SSS+LAGGKFYPLINAV
Sbjct: 306 GPGPGTVSNISPWMFTVAASSIDRDFTSYASLGNKKHYKGSSISSSALAGGKFYPLINAV 365

Query: 388 DARNPNSTDSFAQKCETGSLDPAKVKGKIVVCLRGVTARVEKGYVVLQAGGVGMILVNDK 447
           DA+  N+++  AQ C  GSLDP K KGKI+VCLRG  ARVEKG+VVLQAGGVGMILVN K
Sbjct: 366 DAKAANASEILAQLCHKGSLDPTKAKGKIIVCLRGENARVEKGFVVLQAGGVGMILVNGK 425

Query: 448 TDGSGLISDSHILPATSLTYTDGLAVAQYINSTTTPVALITPVETQLGVKPSPVMAGFSS 507
             GSG  +D+HILPAT L+YTDGLAVAQYINST TPVA ITPV+TQLG+KPSPVMA FSS
Sbjct: 426 NGGSGTTADAHILPATHLSYTDGLAVAQYINSTKTPVAHITPVQTQLGIKPSPVMADFSS 485

Query: 508 RGPNPITDAMLKPDITGPGVNILASVTTDVTATKFPFDTRRVPFNVESGTSMSCPHIAGV 567
           RGPNPIT+AMLKPDITGPG++ILASVTTDVTAT FPFDTRRVPFNVESGTSMSCPHI+GV
Sbjct: 486 RGPNPITEAMLKPDITGPGMSILASVTTDVTATTFPFDTRRVPFNVESGTSMSCPHISGV 545

Query: 568 AGLLKTLYPTWSPAAIKSAIMTTAKTRDNSMHTLSDTVKAKATPFDYGAGHVNPNNAMDP 627
            GLLKTLYPTWSPAAIKSAIMTTAKTRDN+M T+SD VK KATPFDYGAGHV+PN+AMDP
Sbjct: 546 VGLLKTLYPTWSPAAIKSAIMTTAKTRDNTMRTISDNVKPKATPFDYGAGHVHPNSAMDP 605

Query: 628 GLVYDTTIDDYLNFLCARGYNAQTLMSFHSKPFVCATPFTLTDLNYPSISIPELKSGAPV 687
           GLVYDTTIDDYLNFLCARGYN+ T  +F++KPFVCA  FTLTDLNYPSISIP+L+ GAPV
Sbjct: 606 GLVYDTTIDDYLNFLCARGYNSLTFKNFYNKPFVCAKSFTLTDLNYPSISIPKLQFGAPV 665

Query: 688 TVNRRVKNVGTPGTYVARVKASSKISVTVEPSTLQFNSVGEEKAFKVVFEYKGTGQGKGY 747
           TVNRRVKNVGTPGTYVARV ASSKI VTVEPSTLQFNSVGEEKAFKVVFEYKG  Q KGY
Sbjct: 666 TVNRRVKNVGTPGTYVARVNASSKILVTVEPSTLQFNSVGEEKAFKVVFEYKGNEQDKGY 725

Query: 748 VFGTLIWSDNGKHNVRSPIVVKLG 772
           VFGTLIWSD GKHNVRSPIVV LG
Sbjct: 726 VFGTLIWSD-GKHNVRSPIVVNLG 746

BLAST of IVF0023697 vs. ExPASy TrEMBL
Match: A0A6J1GDW2 (subtilisin-like protease SBT5.3 OS=Cucurbita moschata OX=3662 GN=LOC111453052 PE=3 SV=1)

HSP 1 Score: 1115.9 bits (2885), Expect = 0.0e+00
Identity = 548/771 (71.08%), Postives = 644/771 (83.53%), Query Frame = 0

Query: 3   YFSYIVSPLLLFFFLLQTSALPTKKSYIVYLGAHSFGPNPTNFDIEAATESHYDLLGSVV 62
           Y SY++SPLLLFFF+LQT+A+PT+KSYIVYLG+HSFGPNP+ +D++ ATES YD+LGSV 
Sbjct: 6   YISYVISPLLLFFFVLQTAAVPTQKSYIVYLGSHSFGPNPSIYDVQLATESQYDILGSVK 65

Query: 63  GNNLEAKDSIKYSYNKYINGFAAVLDEQQATDLAKNPKVVSVFENKKRQLHTTRSWSFLG 122
           G+ + AKDSI YSYN+YINGFAAVLD+Q+AT LAKNP VVS+FENK+R+LHTTRSWSFLG
Sbjct: 66  GSKVAAKDSILYSYNRYINGFAAVLDDQEATALAKNPSVVSIFENKERKLHTTRSWSFLG 125

Query: 123 MENDEGIPSNSIWKAARFGEDTIIGNLDSGVWPESKSFNDAGYGPVPSRWRGACEGGNNF 182
           +++D GIP NSIWKAARFGEDTIIGNLD+GVWPES SFNDAGYGPVP+RWRGAC+GG+ F
Sbjct: 126 VDSDRGIPRNSIWKAARFGEDTIIGNLDTGVWPESPSFNDAGYGPVPARWRGACDGGSKF 185

Query: 183 QCNRKLIGARYFHAGFVMGS---GLINVSFNKTARDEDGHGSHTLSTAGGNFVPGASIFG 242
           +CNRKLIGARYF+ GF          N+SF+ +A+D +GHGSHTLSTAGGNFV G ++FG
Sbjct: 186 RCNRKLIGARYFYRGFQASERPWTKQNISFD-SAQDHEGHGSHTLSTAGGNFVHGVNVFG 245

Query: 243 YGNGTAKGGSPKARVAAYRVCWPPALFGGGCYDADILAGFEAAISDGVDVLSVSLGSEAQ 302
            GNGTAKGGSP+ARV AY+VCWP     GGCYD+DILAG EAAISDGVDVLS SLG  AQ
Sbjct: 246 NGNGTAKGGSPRARVVAYKVCWPSE--NGGCYDSDILAGIEAAISDGVDVLSASLGMAAQ 305

Query: 303 EFSHDSMSIGAFHAVEQGIVVVCSAGNSGPGPKTVSNVSPWMFTVGASTIDRDFTSYATL 362
           EF+HD++SIGAFHAV+ GIVVVCSAGN GP P +VSNVSPWM TVGASTIDRDF SY  L
Sbjct: 306 EFAHDAISIGAFHAVQHGIVVVCSAGNDGPSPGSVSNVSPWMVTVGASTIDRDFVSYVAL 365

Query: 363 GNKKQYKGSSLSSSSLAGGKFYPLINAVDARNPNSTDSFAQKCETGSLDPAKVKGKIVVC 422
           GNKKQ +GSSLSSS L  GKFYPLI AV  +  N+TD FAQ C  G+LDP K KGKI+VC
Sbjct: 366 GNKKQLRGSSLSSSRLPAGKFYPLIKAVQVKAANATDGFAQLCMDGTLDPTKAKGKIIVC 425

Query: 423 LRGVTARVEKGYVVLQAGGVGMILVNDKTDGSGLISDSHILPATSLTYTDGLAVAQYINS 482
           LRG  ARV KG+ V + GG+GM+LVN++ DGS +++D HILPA+ L+  DG+++ QY++S
Sbjct: 426 LRGENARVSKGFEVHRVGGIGMVLVNNQIDGSAIVADPHILPASHLSDADGVSITQYLSS 485

Query: 483 TTTPVALITPVETQLGVKPSPVMAGFSSRGPNPITDAMLKPDITGPGVNILASVTTDVTA 542
           T TPVA IT   T++G+KPSP+MA FSSRGP+ IT+A++KPDIT PGVNI+ASVT D+TA
Sbjct: 486 TKTPVASITHASTEMGIKPSPLMASFSSRGPDFITEAVIKPDITAPGVNIIASVTNDITA 545

Query: 543 TKFPFDTRRVPFNVESGTSMSCPHIAGVAGLLKTLYPTWSPAAIKSAIMTTAKTRDNSMH 602
           +  PFD RRVPFN+ESGTSMSCPHI+GVAGLLKTL+PTWSP AIKSAIMTTAKTRDN+ +
Sbjct: 546 SGLPFDKRRVPFNIESGTSMSCPHISGVAGLLKTLHPTWSPGAIKSAIMTTAKTRDNTKN 605

Query: 603 TLSDTVKAKATPFDYGAGHVNPNNAMDPGLVYDTTIDDYLNFLCARGYNAQTLMSFHSKP 662
           T+ D  K KATPFDYGAGHV+PNNAMDPGLVYDTT+DDYLNFLC RGYN++TL  F +KP
Sbjct: 606 TILDYTKVKATPFDYGAGHVHPNNAMDPGLVYDTTVDDYLNFLCTRGYNSRTLKKFSNKP 665

Query: 663 FVCATPFTLTDLNYPSISIPELKSGAPVTVNRRVKNVGTPGTYVARVKASSKISVTVEPS 722
           FVCA  F  TD NYPSI +P L+ G  VTVNRRVKNVG+ GTYVARVK    I+V VEPS
Sbjct: 666 FVCAKNFATTDFNYPSILVPRLQIGESVTVNRRVKNVGSAGTYVARVKMPKGITVMVEPS 725

Query: 723 TLQFNSVGEEKAFKVVFEYKGTGQGKGYVFGTLIWSDNGKHNVRSPIVVKL 771
           TLQF+SVGEEK FK+VF Y    + +GYVFG L+WSD GKH VRS I V L
Sbjct: 726 TLQFHSVGEEKPFKLVFHYAHKLRRQGYVFGALVWSD-GKHFVRSSIAVNL 772

BLAST of IVF0023697 vs. ExPASy TrEMBL
Match: A0A6J1KUL8 (uncharacterized protein LOC111498820 OS=Cucurbita maxima OX=3661 GN=LOC111498820 PE=3 SV=1)

HSP 1 Score: 1106.7 bits (2861), Expect = 0.0e+00
Identity = 542/766 (70.76%), Postives = 639/766 (83.42%), Query Frame = 0

Query: 8    VSPLLLFFFLLQTSALPTKKSYIVYLGAHSFGPNPTNFDIEAATESHYDLLGSVVGNNLE 67
            +SPLLLFFF+L T+A+PTK SYIVYLG+HSF PNP+ +D++ ATES YD+LGSV G+ + 
Sbjct: 774  ISPLLLFFFMLHTAAVPTKNSYIVYLGSHSFAPNPSVYDVQLATESQYDILGSVKGSKVA 833

Query: 68   AKDSIKYSYNKYINGFAAVLDEQQATDLAKNPKVVSVFENKKRQLHTTRSWSFLGMENDE 127
            AKDSI YSYN+YINGFAAVLDEQ+AT LAKNP VVSVFENK+R+LHTTRSW FLG+++D 
Sbjct: 834  AKDSILYSYNRYINGFAAVLDEQEATALAKNPSVVSVFENKERKLHTTRSWGFLGVDSDR 893

Query: 128  GIPSNSIWKAARFGEDTIIGNLDSGVWPESKSFNDAGYGPVPSRWRGACEGGNNFQCNRK 187
            GIP NSIWKAARFG DTIIGNLD+GVWPES SFNDAGYGPVPSRWRGACEGG+ F+CNRK
Sbjct: 894  GIPQNSIWKAARFGADTIIGNLDTGVWPESPSFNDAGYGPVPSRWRGACEGGSKFRCNRK 953

Query: 188  LIGARYFHAGFVMGSGLI---NVSFNKTARDEDGHGSHTLSTAGGNFVPGASIFGYGNGT 247
            LIGARYF+ GF    G +   N+SF+ +ARD +GHGSHTLSTAGGNFV G ++FG GNGT
Sbjct: 954  LIGARYFYRGFQAAEGPLTTHNISFD-SARDHEGHGSHTLSTAGGNFVHGVNVFGNGNGT 1013

Query: 248  AKGGSPKARVAAYRVCWPPALFGGGCYDADILAGFEAAISDGVDVLSVSLGSEAQEFSHD 307
            AKGGSP+ARV AY+VCWP     GGCYD+DILAG EAAISDGVDVLS S+G+ AQEF++D
Sbjct: 1014 AKGGSPRARVVAYKVCWPSK--NGGCYDSDILAGIEAAISDGVDVLSASIGTLAQEFAND 1073

Query: 308  SMSIGAFHAVEQGIVVVCSAGNSGPGPKTVSNVSPWMFTVGASTIDRDFTSYATLGNKKQ 367
            ++SIGAFHAV+ GIVVVCSAGN GP P +VSNVSPWM TVGASTIDRDF SY  LGNKK+
Sbjct: 1074 AISIGAFHAVQHGIVVVCSAGNDGPSPGSVSNVSPWMVTVGASTIDRDFVSYVVLGNKKR 1133

Query: 368  YKGSSLSSSSLAGGKFYPLINAVDARNPNSTDSFAQKCETGSLDPAKVKGKIVVCLRGVT 427
            ++GSSLSSS L  GKFYPLI AV  +  N+TD FAQ C  G+LDP K KGKI+VCLRG  
Sbjct: 1134 FRGSSLSSSRLPAGKFYPLIKAVQVKAANATDGFAQLCMDGTLDPTKAKGKIIVCLRGEN 1193

Query: 428  ARVEKGYVVLQAGGVGMILVNDKTDGSGLISDSHILPATSLTYTDGLAVAQYINSTTTPV 487
            ARV KG+ V + GGVGM+LVN++ DGS L++D HILPA+ L+Y DG+++AQY++ST TPV
Sbjct: 1194 ARVSKGFEVFRVGGVGMVLVNNQMDGSALVADPHILPASHLSYADGVSIAQYLSSTKTPV 1253

Query: 488  ALITPVETQLGVKPSPVMAGFSSRGPNPITDAMLKPDITGPGVNILASVTTDVTATKFPF 547
            A IT   T++G+KPSP+MA FSSRGP+ IT A++KPDIT PGVNI+ASVT D++A+  P 
Sbjct: 1254 ASITHASTEMGIKPSPLMASFSSRGPDFITQAVIKPDITAPGVNIIASVTDDISASGLPL 1313

Query: 548  DTRRVPFNVESGTSMSCPHIAGVAGLLKTLYPTWSPAAIKSAIMTTAKTRDNSMHTLSDT 607
            D RRVPFN+ESGTSMSCPHI+GVAGLLKTL+PTWSPAAIKSA+MTTAKTRDN+ +TL D 
Sbjct: 1314 DKRRVPFNIESGTSMSCPHISGVAGLLKTLHPTWSPAAIKSAVMTTAKTRDNTKNTLLDY 1373

Query: 608  VKAKATPFDYGAGHVNPNNAMDPGLVYDTTIDDYLNFLCARGYNAQTLMSFHSKPFVCAT 667
             K KATPFDYGAGHV+PN+AMDPGLVYDTT+DDYLNFLC RGYN++TL  F +KPFVCA 
Sbjct: 1374 TKVKATPFDYGAGHVHPNDAMDPGLVYDTTVDDYLNFLCTRGYNSRTLKKFSNKPFVCAN 1433

Query: 668  PFTLTDLNYPSISIPELKSGAPVTVNRRVKNVGTPGTYVARVKASSKISVTVEPSTLQFN 727
             F  TD NYPSI +P L+ G  VTVNRRVKNVG+ GTYVARV+    I+V VEPS LQF+
Sbjct: 1434 NFATTDFNYPSILVPRLQIGGSVTVNRRVKNVGSTGTYVARVRMPKGITVMVEPSKLQFH 1493

Query: 728  SVGEEKAFKVVFEYKGTGQGKGYVFGTLIWSDNGKHNVRSPIVVKL 771
            SVGEE+ FK++F Y    + +GYVFG L+WSD GKH VRS I V L
Sbjct: 1494 SVGEERPFKLIFHYAHKLRRQGYVFGALVWSD-GKHFVRSSIAVNL 1535

BLAST of IVF0023697 vs. NCBI nr
Match: XP_008449184.1 (PREDICTED: subtilisin-like protease SBT5.3 [Cucumis melo])

HSP 1 Score: 1536 bits (3978), Expect = 0.0
Identity = 768/771 (99.61%), Postives = 770/771 (99.87%), Query Frame = 0

Query: 1   MEYFSYIVSPLLLFFFLLQTSALPTKKSYIVYLGAHSFGPNPTNFDIEAATESHYDLLGS 60
           MEYFSYIVSPLLLFFFLLQTSALPTKKSYIVYLGAHSFGPNPTNFDIEAATESHYDLLGS
Sbjct: 1   MEYFSYIVSPLLLFFFLLQTSALPTKKSYIVYLGAHSFGPNPTNFDIEAATESHYDLLGS 60

Query: 61  VVGNNLEAKDSIKYSYNKYINGFAAVLDEQQATDLAKNPKVVSVFENKKRQLHTTRSWSF 120
           VVGNNLEAKDSIKYSYNKYINGFAAVLDEQQATDLAKNPKVVSVFENKKRQLHTTRSWSF
Sbjct: 61  VVGNNLEAKDSIKYSYNKYINGFAAVLDEQQATDLAKNPKVVSVFENKKRQLHTTRSWSF 120

Query: 121 LGMENDEGIPSNSIWKAARFGEDTIIGNLDSGVWPESKSFNDAGYGPVPSRWRGACEGGN 180
           LGMENDEGIPSNSIWKAARFGEDTIIGNLDSGVWPESKSFNDAGYGPVPSRWRGACEGGN
Sbjct: 121 LGMENDEGIPSNSIWKAARFGEDTIIGNLDSGVWPESKSFNDAGYGPVPSRWRGACEGGN 180

Query: 181 NFQCNRKLIGARYFHAGFVMGSGLINVSFNKTARDEDGHGSHTLSTAGGNFVPGASIFGY 240
           NFQCNRKLIGARYFHAGFVMGSGLINVSFNKTARDEDGHGSHTLSTAGGNFVPGASIFGY
Sbjct: 181 NFQCNRKLIGARYFHAGFVMGSGLINVSFNKTARDEDGHGSHTLSTAGGNFVPGASIFGY 240

Query: 241 GNGTAKGGSPKARVAAYRVCWPPALFGGGCYDADILAGFEAAISDGVDVLSVSLGSEAQE 300
           GNGTAKGGSPKARVAAYRVCWPPALFGGGCYDADILAGFEAAISDGVDVLSVSLGSEAQE
Sbjct: 241 GNGTAKGGSPKARVAAYRVCWPPALFGGGCYDADILAGFEAAISDGVDVLSVSLGSEAQE 300

Query: 301 FSHDSMSIGAFHAVEQGIVVVCSAGNSGPGPKTVSNVSPWMFTVGASTIDRDFTSYATLG 360
           FSHDSMSIGAFHAVEQGIVVVCSAGNSGPGPKTVSNVSPWMFTVGASTIDRDFTSYATLG
Sbjct: 301 FSHDSMSIGAFHAVEQGIVVVCSAGNSGPGPKTVSNVSPWMFTVGASTIDRDFTSYATLG 360

Query: 361 NKKQYKGSSLSSSSLAGGKFYPLINAVDARNPNSTDSFAQKCETGSLDPAKVKGKIVVCL 420
           NKKQYKGSSLSSSSLAGGKFYPLINAVDARNPNSTDSFAQKCETGSLDPAKVKGKIVVCL
Sbjct: 361 NKKQYKGSSLSSSSLAGGKFYPLINAVDARNPNSTDSFAQKCETGSLDPAKVKGKIVVCL 420

Query: 421 RGVTARVEKGYVVLQAGGVGMILVNDKTDGSGLISDSHILPATSLTYTDGLAVAQYINST 480
           RGVTARVEKGYVVLQAGGVGMILVNDKTDGSGLISDSHILPATSLTYTDGLAVAQYINST
Sbjct: 421 RGVTARVEKGYVVLQAGGVGMILVNDKTDGSGLISDSHILPATSLTYTDGLAVAQYINST 480

Query: 481 TTPVALITPVETQLGVKPSPVMAGFSSRGPNPITDAMLKPDITGPGVNILASVTTDVTAT 540
           TTPVALITPVETQLGVKPSPVMAGFSSRGPNPITDAMLKPDITGPGVNILASVTTDVTAT
Sbjct: 481 TTPVALITPVETQLGVKPSPVMAGFSSRGPNPITDAMLKPDITGPGVNILASVTTDVTAT 540

Query: 541 KFPFDTRRVPFNVESGTSMSCPHIAGVAGLLKTLYPTWSPAAIKSAIMTTAKTRDNSMHT 600
           KFPFDTRRVPFNVESGTSMSCPHIAGV+GLLKTLYPTWSPAAIKSAIMTTAKTRDNSMHT
Sbjct: 541 KFPFDTRRVPFNVESGTSMSCPHIAGVSGLLKTLYPTWSPAAIKSAIMTTAKTRDNSMHT 600

Query: 601 LSDTVKAKATPFDYGAGHVNPNNAMDPGLVYDTTIDDYLNFLCARGYNAQTLMSFHSKPF 660
           LSDTVKAKATPFDYGAGHVNPNNAMDPGLVYDTTIDDYLNFLCARGYNAQTLMSFHSKPF
Sbjct: 601 LSDTVKAKATPFDYGAGHVNPNNAMDPGLVYDTTIDDYLNFLCARGYNAQTLMSFHSKPF 660

Query: 661 VCATPFTLTDLNYPSISIPELKSGAPVTVNRRVKNVGTPGTYVARVKASSKISVTVEPST 720
           VCATPFTLTDLNYPSISIPELKSGAPVTVNRRVKNVGTPGTYVARVKASSKISVTVEPST
Sbjct: 661 VCATPFTLTDLNYPSISIPELKSGAPVTVNRRVKNVGTPGTYVARVKASSKISVTVEPST 720

Query: 721 LQFNSVGEEKAFKVVFEYKGTGQGKGYVFGTLIWSDNGKHNVRSPIVVKLG 771
           LQFNSVGEEKAFKV+FEYKGTGQGK YVFGTLIWSDNGKHNVRSPIVVKLG
Sbjct: 721 LQFNSVGEEKAFKVLFEYKGTGQGKSYVFGTLIWSDNGKHNVRSPIVVKLG 771

BLAST of IVF0023697 vs. NCBI nr
Match: TYK19386.1 (subtilisin-like protease SBT5.3 [Cucumis melo var. makuwa])

HSP 1 Score: 1292 bits (3343), Expect = 0.0
Identity = 644/644 (100.00%), Postives = 644/644 (100.00%), Query Frame = 0

Query: 123 MENDEGIPSNSIWKAARFGEDTIIGNLDSGVWPESKSFNDAGYGPVPSRWRGACEGGNNF 182
           MENDEGIPSNSIWKAARFGEDTIIGNLDSGVWPESKSFNDAGYGPVPSRWRGACEGGNNF
Sbjct: 1   MENDEGIPSNSIWKAARFGEDTIIGNLDSGVWPESKSFNDAGYGPVPSRWRGACEGGNNF 60

Query: 183 QCNRKLIGARYFHAGFVMGSGLINVSFNKTARDEDGHGSHTLSTAGGNFVPGASIFGYGN 242
           QCNRKLIGARYFHAGFVMGSGLINVSFNKTARDEDGHGSHTLSTAGGNFVPGASIFGYGN
Sbjct: 61  QCNRKLIGARYFHAGFVMGSGLINVSFNKTARDEDGHGSHTLSTAGGNFVPGASIFGYGN 120

Query: 243 GTAKGGSPKARVAAYRVCWPPALFGGGCYDADILAGFEAAISDGVDVLSVSLGSEAQEFS 302
           GTAKGGSPKARVAAYRVCWPPALFGGGCYDADILAGFEAAISDGVDVLSVSLGSEAQEFS
Sbjct: 121 GTAKGGSPKARVAAYRVCWPPALFGGGCYDADILAGFEAAISDGVDVLSVSLGSEAQEFS 180

Query: 303 HDSMSIGAFHAVEQGIVVVCSAGNSGPGPKTVSNVSPWMFTVGASTIDRDFTSYATLGNK 362
           HDSMSIGAFHAVEQGIVVVCSAGNSGPGPKTVSNVSPWMFTVGASTIDRDFTSYATLGNK
Sbjct: 181 HDSMSIGAFHAVEQGIVVVCSAGNSGPGPKTVSNVSPWMFTVGASTIDRDFTSYATLGNK 240

Query: 363 KQYKGSSLSSSSLAGGKFYPLINAVDARNPNSTDSFAQKCETGSLDPAKVKGKIVVCLRG 422
           KQYKGSSLSSSSLAGGKFYPLINAVDARNPNSTDSFAQKCETGSLDPAKVKGKIVVCLRG
Sbjct: 241 KQYKGSSLSSSSLAGGKFYPLINAVDARNPNSTDSFAQKCETGSLDPAKVKGKIVVCLRG 300

Query: 423 VTARVEKGYVVLQAGGVGMILVNDKTDGSGLISDSHILPATSLTYTDGLAVAQYINSTTT 482
           VTARVEKGYVVLQAGGVGMILVNDKTDGSGLISDSHILPATSLTYTDGLAVAQYINSTTT
Sbjct: 301 VTARVEKGYVVLQAGGVGMILVNDKTDGSGLISDSHILPATSLTYTDGLAVAQYINSTTT 360

Query: 483 PVALITPVETQLGVKPSPVMAGFSSRGPNPITDAMLKPDITGPGVNILASVTTDVTATKF 542
           PVALITPVETQLGVKPSPVMAGFSSRGPNPITDAMLKPDITGPGVNILASVTTDVTATKF
Sbjct: 361 PVALITPVETQLGVKPSPVMAGFSSRGPNPITDAMLKPDITGPGVNILASVTTDVTATKF 420

Query: 543 PFDTRRVPFNVESGTSMSCPHIAGVAGLLKTLYPTWSPAAIKSAIMTTAKTRDNSMHTLS 602
           PFDTRRVPFNVESGTSMSCPHIAGVAGLLKTLYPTWSPAAIKSAIMTTAKTRDNSMHTLS
Sbjct: 421 PFDTRRVPFNVESGTSMSCPHIAGVAGLLKTLYPTWSPAAIKSAIMTTAKTRDNSMHTLS 480

Query: 603 DTVKAKATPFDYGAGHVNPNNAMDPGLVYDTTIDDYLNFLCARGYNAQTLMSFHSKPFVC 662
           DTVKAKATPFDYGAGHVNPNNAMDPGLVYDTTIDDYLNFLCARGYNAQTLMSFHSKPFVC
Sbjct: 481 DTVKAKATPFDYGAGHVNPNNAMDPGLVYDTTIDDYLNFLCARGYNAQTLMSFHSKPFVC 540

Query: 663 ATPFTLTDLNYPSISIPELKSGAPVTVNRRVKNVGTPGTYVARVKASSKISVTVEPSTLQ 722
           ATPFTLTDLNYPSISIPELKSGAPVTVNRRVKNVGTPGTYVARVKASSKISVTVEPSTLQ
Sbjct: 541 ATPFTLTDLNYPSISIPELKSGAPVTVNRRVKNVGTPGTYVARVKASSKISVTVEPSTLQ 600

Query: 723 FNSVGEEKAFKVVFEYKGTGQGKGYVFGTLIWSDNGKHNVRSPI 766
           FNSVGEEKAFKVVFEYKGTGQGKGYVFGTLIWSDNGKHNVRSPI
Sbjct: 601 FNSVGEEKAFKVVFEYKGTGQGKGYVFGTLIWSDNGKHNVRSPI 644

BLAST of IVF0023697 vs. NCBI nr
Match: XP_031738719.1 (uncharacterized protein LOC101212014 [Cucumis sativus])

HSP 1 Score: 1261 bits (3264), Expect = 0.0
Identity = 627/745 (84.16%), Postives = 680/745 (91.28%), Query Frame = 0

Query: 27  KSYIVYLGAHSFGPNPTNFDIEAATESHYDLLGSVVGNNLEAKDSIKYSYNKYINGFAAV 86
           +SYIVYLGA S G NPTN+DIE ATES YDLLGSVVG+ L AKD+IKYSYNKYINGFAA 
Sbjct: 54  ESYIVYLGAPSVGSNPTNYDIEVATESQYDLLGSVVGSKLAAKDAIKYSYNKYINGFAAT 113

Query: 87  LDEQQATDLAKNPKVVSVFENKKRQLHTTRSWSFLGMENDEGIPSNSIWKAARFGEDTII 146
           LDE+QA DLAKNPKVVSVFENK+R+LHTTRSW FLG+E+DEGIPSNSIW A RFGEDTII
Sbjct: 114 LDEKQAKDLAKNPKVVSVFENKERKLHTTRSWHFLGVESDEGIPSNSIWNAGRFGEDTII 173

Query: 147 GNLDSGVWPESKSFNDAGYGPVPSRWRGACEGGNNFQCNRKLIGARYFHAGFVMGSGLIN 206
           GNLD+GVWPESKSFNDAGYGPVPSRWRGACEGG NF+CNRKLIGARYF+ GF M SG +N
Sbjct: 174 GNLDTGVWPESKSFNDAGYGPVPSRWRGACEGGANFRCNRKLIGARYFNKGFAMASGPLN 233

Query: 207 VSFNKTARDEDGHGSHTLSTAGGNFVPGASIFGYGNGTAKGGSPKARVAAYRVCWPPALF 266
           +SFN TARD+ GHGSHTLSTAGGNFVPGA++FGYGNGTAKGGSPKARVAAY+VCWP A  
Sbjct: 234 ISFN-TARDKQGHGSHTLSTAGGNFVPGANVFGYGNGTAKGGSPKARVAAYKVCWP-ATS 293

Query: 267 GGGCYDADILAGFEAAISDGVDVLSVSLGSEAQEFSHDSMSIGAFHAVEQGIVVVCSAGN 326
           GGGCYDADILAGFEAAISDGVDVLSVSLGS+ +EF++DSMSIGAFHAV+QGIVVVCSAGN
Sbjct: 294 GGGCYDADILAGFEAAISDGVDVLSVSLGSKPEEFAYDSMSIGAFHAVQQGIVVVCSAGN 353

Query: 327 SGPGPKTVSNVSPWMFTVGASTIDRDFTSYATLGNKKQYKGSSLSSSSLAGGKFYPLINA 386
            GPGP TVSN+SPWMFTV AS+IDRDFTSYA+LGNKK YKGSS+SSS+LAGGKFYPLINA
Sbjct: 354 DGPGPGTVSNISPWMFTVAASSIDRDFTSYASLGNKKHYKGSSISSSALAGGKFYPLINA 413

Query: 387 VDARNPNSTDSFAQKCETGSLDPAKVKGKIVVCLRGVTARVEKGYVVLQAGGVGMILVND 446
           VDA+  N+++  AQ C  GSLDP K KGKI+VCLRG  ARVEKG+VVLQAGGVGMILVN 
Sbjct: 414 VDAKAANASEILAQLCHKGSLDPTKAKGKIIVCLRGENARVEKGFVVLQAGGVGMILVNG 473

Query: 447 KTDGSGLISDSHILPATSLTYTDGLAVAQYINSTTTPVALITPVETQLGVKPSPVMAGFS 506
           K  GSG  +D+HILPAT L+YTDGLAVAQYINST TPVA ITPV+TQLG+KPSPVMA FS
Sbjct: 474 KNGGSGTTADAHILPATHLSYTDGLAVAQYINSTKTPVAHITPVQTQLGIKPSPVMADFS 533

Query: 507 SRGPNPITDAMLKPDITGPGVNILASVTTDVTATKFPFDTRRVPFNVESGTSMSCPHIAG 566
           SRGPNPIT+AMLKPDITGPG++ILASVTTDVTAT FPFDTRRVPFNVESGTSMSCPHI+G
Sbjct: 534 SRGPNPITEAMLKPDITGPGMSILASVTTDVTATTFPFDTRRVPFNVESGTSMSCPHISG 593

Query: 567 VAGLLKTLYPTWSPAAIKSAIMTTAKTRDNSMHTLSDTVKAKATPFDYGAGHVNPNNAMD 626
           V GLLKTLYPTWSPAAIKSAIMTTAKTRDN+M T+SD VK KATPFDYGAGHV+PN+AMD
Sbjct: 594 VVGLLKTLYPTWSPAAIKSAIMTTAKTRDNTMRTISDNVKPKATPFDYGAGHVHPNSAMD 653

Query: 627 PGLVYDTTIDDYLNFLCARGYNAQTLMSFHSKPFVCATPFTLTDLNYPSISIPELKSGAP 686
           PGLVYDTTIDDYLNFLCARGYN+ T  +F++KPFVCA  FTLTDLNYPSISIP+L+ GAP
Sbjct: 654 PGLVYDTTIDDYLNFLCARGYNSLTFKNFYNKPFVCAKSFTLTDLNYPSISIPKLQFGAP 713

Query: 687 VTVNRRVKNVGTPGTYVARVKASSKISVTVEPSTLQFNSVGEEKAFKVVFEYKGTGQGKG 746
           VTVNRRVKNVGTPGTYVARV ASSKI VTVEPSTLQFNSVGEEKAFKVVFEYKG  Q KG
Sbjct: 714 VTVNRRVKNVGTPGTYVARVNASSKILVTVEPSTLQFNSVGEEKAFKVVFEYKGNEQDKG 773

Query: 747 YVFGTLIWSDNGKHNVRSPIVVKLG 771
           YVFGTLIWSD GKHNVRSPIVV LG
Sbjct: 774 YVFGTLIWSD-GKHNVRSPIVVNLG 795

BLAST of IVF0023697 vs. NCBI nr
Match: KAE8650093.1 (hypothetical protein Csa_009608 [Cucumis sativus])

HSP 1 Score: 1252 bits (3240), Expect = 0.0
Identity = 627/758 (82.72%), Postives = 680/758 (89.71%), Query Frame = 0

Query: 27  KSYIVYLGAHSFGPNPTNFDIEAATESHYDLLGSVVGNNLEAKDSIKYSYNKYINGFAAV 86
           +SYIVYLGA S G NPTN+DIE ATES YDLLGSVVG+ L AKD+IKYSYNKYINGFAA 
Sbjct: 69  ESYIVYLGAPSVGSNPTNYDIEVATESQYDLLGSVVGSKLAAKDAIKYSYNKYINGFAAT 128

Query: 87  LDEQQATDLAKNPKVVSVFENKKRQLHTTRSWSFLGMENDEGIPSNSIWKAARFGEDTII 146
           LDE+QA DLAKNPKVVSVFENK+R+LHTTRSW FLG+E+DEGIPSNSIW A RFGEDTII
Sbjct: 129 LDEKQAKDLAKNPKVVSVFENKERKLHTTRSWHFLGVESDEGIPSNSIWNAGRFGEDTII 188

Query: 147 GNLDSGVWPESKSFNDAGYGPVPSRWRGACEGGNNFQCNRKLIGARYFHAGFVMGSGLIN 206
           GNLD+GVWPESKSFNDAGYGPVPSRWRGACEGG NF+CNRKLIGARYF+ GF M SG +N
Sbjct: 189 GNLDTGVWPESKSFNDAGYGPVPSRWRGACEGGANFRCNRKLIGARYFNKGFAMASGPLN 248

Query: 207 VSFNKTARDEDGHGSHTLSTAGGNFVPGASIFGYGNGTAKGGSPKARVAAYRVCWPPALF 266
           +SFN TARD+ GHGSHTLSTAGGNFVPGA++FGYGNGTAKGGSPKARVAAY+VCWP A  
Sbjct: 249 ISFN-TARDKQGHGSHTLSTAGGNFVPGANVFGYGNGTAKGGSPKARVAAYKVCWP-ATS 308

Query: 267 GGGCYDADILAGFEAAISDGVDVLSVSLGSEAQEFSHDSMSIGAFHAVEQGIVVVCSAGN 326
           GGGCYDADILAGFEAAISDGVDVLSVSLGS+ +EF++DSMSIGAFHAV+QGIVVVCSAGN
Sbjct: 309 GGGCYDADILAGFEAAISDGVDVLSVSLGSKPEEFAYDSMSIGAFHAVQQGIVVVCSAGN 368

Query: 327 SGPGPKTVSNVSPWMFTVGASTIDRDFTSYATLGNKKQYK-------------GSSLSSS 386
            GPGP TVSN+SPWMFTV AS+IDRDFTSYA+LGNKK YK             GSS+SSS
Sbjct: 369 DGPGPGTVSNISPWMFTVAASSIDRDFTSYASLGNKKHYKSFALVNLKLYTFQGSSISSS 428

Query: 387 SLAGGKFYPLINAVDARNPNSTDSFAQKCETGSLDPAKVKGKIVVCLRGVTARVEKGYVV 446
           +LAGGKFYPLINAVDA+  N+++  AQ C  GSLDP K KGKI+VCLRG  ARVEKG+VV
Sbjct: 429 ALAGGKFYPLINAVDAKAANASEILAQLCHKGSLDPTKAKGKIIVCLRGENARVEKGFVV 488

Query: 447 LQAGGVGMILVNDKTDGSGLISDSHILPATSLTYTDGLAVAQYINSTTTPVALITPVETQ 506
           LQAGGVGMILVN K  GSG  +D+HILPAT L+YTDGLAVAQYINST TPVA ITPV+TQ
Sbjct: 489 LQAGGVGMILVNGKNGGSGTTADAHILPATHLSYTDGLAVAQYINSTKTPVAHITPVQTQ 548

Query: 507 LGVKPSPVMAGFSSRGPNPITDAMLKPDITGPGVNILASVTTDVTATKFPFDTRRVPFNV 566
           LG+KPSPVMA FSSRGPNPIT+AMLKPDITGPG++ILASVTTDVTAT FPFDTRRVPFNV
Sbjct: 549 LGIKPSPVMADFSSRGPNPITEAMLKPDITGPGMSILASVTTDVTATTFPFDTRRVPFNV 608

Query: 567 ESGTSMSCPHIAGVAGLLKTLYPTWSPAAIKSAIMTTAKTRDNSMHTLSDTVKAKATPFD 626
           ESGTSMSCPHI+GV GLLKTLYPTWSPAAIKSAIMTTAKTRDN+M T+SD VK KATPFD
Sbjct: 609 ESGTSMSCPHISGVVGLLKTLYPTWSPAAIKSAIMTTAKTRDNTMRTISDNVKPKATPFD 668

Query: 627 YGAGHVNPNNAMDPGLVYDTTIDDYLNFLCARGYNAQTLMSFHSKPFVCATPFTLTDLNY 686
           YGAGHV+PN+AMDPGLVYDTTIDDYLNFLCARGYN+ T  +F++KPFVCA  FTLTDLNY
Sbjct: 669 YGAGHVHPNSAMDPGLVYDTTIDDYLNFLCARGYNSLTFKNFYNKPFVCAKSFTLTDLNY 728

Query: 687 PSISIPELKSGAPVTVNRRVKNVGTPGTYVARVKASSKISVTVEPSTLQFNSVGEEKAFK 746
           PSISIP+L+ GAPVTVNRRVKNVGTPGTYVARV ASSKI VTVEPSTLQFNSVGEEKAFK
Sbjct: 729 PSISIPKLQFGAPVTVNRRVKNVGTPGTYVARVNASSKILVTVEPSTLQFNSVGEEKAFK 788

Query: 747 VVFEYKGTGQGKGYVFGTLIWSDNGKHNVRSPIVVKLG 771
           VVFEYKG  Q KGYVFGTLIWSD GKHNVRSPIVV LG
Sbjct: 789 VVFEYKGNEQDKGYVFGTLIWSD-GKHNVRSPIVVNLG 823

BLAST of IVF0023697 vs. NCBI nr
Match: XP_038905686.1 (subtilisin-like protease SBT5.3 [Benincasa hispida])

HSP 1 Score: 1226 bits (3171), Expect = 0.0
Identity = 605/768 (78.78%), Postives = 682/768 (88.80%), Query Frame = 0

Query: 4   FSYIVSPLLLFFFLLQTSALPTKKSYIVYLGAHSFGPNPTNFDIEAATESHYDLLGSVVG 63
           FSY +SPLLLFFFLLQTSA+PTKKSYIVYLG HSFG NP+ +D++ ATES YD+L SV G
Sbjct: 3   FSYRLSPLLLFFFLLQTSAIPTKKSYIVYLGLHSFGLNPSKYDVQRATESQYDILSSVTG 62

Query: 64  NNLEAKDSIKYSYNKYINGFAAVLDEQQATDLAKNPKVVSVFENKKRQLHTTRSWSFLGM 123
           + L AK+SI YSY++YINGFAAVLDE++A  LAKNP VVSVFENK+R+LHTT+SWSFLG+
Sbjct: 63  SKLAAKESIMYSYSRYINGFAAVLDEKEAMALAKNPSVVSVFENKERKLHTTKSWSFLGV 122

Query: 124 ENDEGIPSNSIWKAARFGEDTIIGNLDSGVWPESKSFNDAGYGPVPSRWRGACEGGNNFQ 183
           ++D GIPSNSIWKAARFGEDTIIGNLD+G WPESKSFNDAGYGPVPSRWRGAC+GG NF+
Sbjct: 123 DSDAGIPSNSIWKAARFGEDTIIGNLDTGAWPESKSFNDAGYGPVPSRWRGACDGGANFR 182

Query: 184 CNRKLIGARYFHAGFVMGSGLINVSFNKTARDEDGHGSHTLSTAGGNFVPGASIFGYGNG 243
           CNRKLIGARYF+ GF M +G +NVSFN TARD++GHGSHTLSTAGGNFVPGA+IFGYGNG
Sbjct: 183 CNRKLIGARYFNQGFAMANGPLNVSFN-TARDKEGHGSHTLSTAGGNFVPGANIFGYGNG 242

Query: 244 TAKGGSPKARVAAYRVCWPPALFGGGCYDADILAGFEAAISDGVDVLSVSLGSEAQEFSH 303
           TAKGGSPKARVAAY+VCWP     GGC+D+DILAGFEAAI DGVDVLSVSLG+ AQEF++
Sbjct: 243 TAKGGSPKARVAAYKVCWPAPT--GGCFDSDILAGFEAAIGDGVDVLSVSLGTGAQEFAY 302

Query: 304 DSMSIGAFHAVEQGIVVVCSAGNSGPGPKTVSNVSPWMFTVGASTIDRDFTSYATLGNKK 363
           D++SIGAFHAV++GIVVVCS GN GP P TVSNVSPWMFTV ASTIDRDF SY  LGNKK
Sbjct: 303 DAVSIGAFHAVQKGIVVVCSGGNDGPSPGTVSNVSPWMFTVAASTIDRDFASYVLLGNKK 362

Query: 364 QYKGSSLSSSSLAGGKFYPLINAVDARNPNSTDSFAQKCETGSLDPAKVKGKIVVCLRGV 423
             +GSSLSSS L G KFYPLINA++A+  N+TDS AQ CE GSLDPAK KGKI+VCLRG 
Sbjct: 363 HIRGSSLSSSGLRGRKFYPLINAIEAKAANATDSLAQFCEQGSLDPAKAKGKIIVCLRGE 422

Query: 424 TARVEKGYVVLQAGGVGMILVNDKTDGSGLISDSHILPATSLTYTDGLAVAQYINSTTTP 483
            ARVEK +VVL AGGVGMI+VND+ DGSG ++D+HILPAT ++YTDGL+++QYI ST TP
Sbjct: 423 NARVEKSFVVLHAGGVGMIMVNDQKDGSGTLADAHILPATHVSYTDGLSISQYIKSTKTP 482

Query: 484 VALITPVETQLGVKPSPVMAGFSSRGPNPITDAMLKPDITGPGVNILASVTTDVTATKFP 543
           VA IT V+T++G+KPSPVMA FSSRGPN IT+AMLKPDIT PGVNI+ASVT D TAT  P
Sbjct: 483 VAYITHVKTEVGIKPSPVMADFSSRGPNSITEAMLKPDITAPGVNIIASVTIDATATDSP 542

Query: 544 FDTRRVPFNVESGTSMSCPHIAGVAGLLKTLYPTWSPAAIKSAIMTTAKTRDNSMHTLSD 603
           FDTRRVPFNVESGTSMSCPHI+GVAGLLKTLYPTWSPAAIKSAIMTTAKTRDN+  T+SD
Sbjct: 543 FDTRRVPFNVESGTSMSCPHISGVAGLLKTLYPTWSPAAIKSAIMTTAKTRDNTQRTISD 602

Query: 604 TVKAKATPFDYGAGHVNPNNAMDPGLVYDTTIDDYLNFLCARGYNAQTLMSFHSKPFVCA 663
           T K KATPFDYGAGHV+PN+AMDPGLVYDTTIDDYLNFLCARGYN+  L  F++KPF+CA
Sbjct: 603 TAKMKATPFDYGAGHVHPNSAMDPGLVYDTTIDDYLNFLCARGYNSVALKKFYNKPFICA 662

Query: 664 TPFTLTDLNYPSISIPELKSGAPVTVNRRVKNVGTPGTYVARVKASSKISVTVEPSTLQF 723
             F +TDLNYPSIS+PEL+ GAPVTVNRRVKNVGTPGTYVARVKAS  ISV+VEPSTLQF
Sbjct: 663 KSFAITDLNYPSISVPELRIGAPVTVNRRVKNVGTPGTYVARVKASPAISVSVEPSTLQF 722

Query: 724 NSVGEEKAFKVVFEYKGTGQGKGYVFGTLIWSDNGKHNVRSPIVVKLG 771
           NSVGEEKAFKVVF+YKG  Q +G+VFGTLIWSD GKH VRSPI VKLG
Sbjct: 723 NSVGEEKAFKVVFQYKGKEQRQGHVFGTLIWSD-GKHFVRSPIAVKLG 766

BLAST of IVF0023697 vs. TAIR 10
Match: AT2G04160.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 855.1 bits (2208), Expect = 4.3e-248
Identity = 439/770 (57.01%), Postives = 549/770 (71.30%), Query Frame = 0

Query: 4   FSYIVSPLLLFFFLLQTSALPTKKSYIVYLGAHSFGPNPTNFDIEAATESHYDLLGSVVG 63
           FS+++  LL+        A     SY+VY GAHS     T   ++   E+HYD LGS  G
Sbjct: 7   FSFLLLLLLVHMSSKHILASKDSSSYVVYFGAHSHVGEITEDAMDRVKETHYDFLGSFTG 66

Query: 64  NNLEAKDSIKYSYNKYINGFAAVLDEQQATDLAKNPKVVSVFENKKRQLHTTRSWSFLGM 123
           +   A D+I YSY K+INGFAA LD   A +++K+P+VVSVF NK  +LHTTRSW FLG+
Sbjct: 67  SRERATDAIFYSYTKHINGFAAHLDHDLAYEISKHPEVVSVFPNKALKLHTTRSWDFLGL 126

Query: 124 ENDEGIPSNSIWKAARFGEDTIIGNLDSGVWPESKSFNDAGYGPVPSRWRGACEGGNN-- 183
           E++  +PS+SIW+ ARFGEDTII NLD+GVWPESKSF D G GP+PSRW+G C+   +  
Sbjct: 127 EHNSYVPSSSIWRKARFGEDTIIANLDTGVWPESKSFRDEGLGPIPSRWKGICQNQKDAT 186

Query: 184 FQCNRKLIGARYFHAGFVMGSGLINVSFNKTARDEDGHGSHTLSTAGGNFVPGASIFGYG 243
           F CNRKLIGARYF+ G+    G +N SF+ + RD DGHGSHTLSTA G+FVPG SIFG G
Sbjct: 187 FHCNRKLIGARYFNKGYAAAVGHLNSSFD-SPRDLDGHGSHTLSTAAGDFVPGVSIFGQG 246

Query: 244 NGTAKGGSPKARVAAYRVCWPPALFGGGCYDADILAGFEAAISDGVDVLSVSLGSEAQEF 303
           NGTAKGGSP+ARVAAY+VCWPP + G  CYDAD+LA F+AAI DG DV+SVSLG E   F
Sbjct: 247 NGTAKGGSPRARVAAYKVCWPP-VKGNECYDADVLAAFDAAIHDGADVISVSLGGEPTSF 306

Query: 304 SHDSMSIGAFHAVEQGIVVVCSAGNSGPGPKTVSNVSPWMFTVGASTIDRDFTSYATLGN 363
            +DS++IG+FHA ++ IVVVCSAGNSGP   TVSNV+PW  TVGAST+DR+F S   LGN
Sbjct: 307 FNDSVAIGSFHAAKKRIVVVCSAGNSGPADSTVSNVAPWQITVGASTMDREFASNLVLGN 366

Query: 364 KKQYKGSSLSSSSLAGGKFYPLINAVDARNPNSTDSFAQKCETGSLDPAKVKGKIVVCLR 423
            K YKG SLSS++L   KFYP++ +V+A+  N++   AQ C+ GSLDP K KGKI+VCLR
Sbjct: 367 GKHYKGQSLSSTALPHAKFYPIMASVNAKAKNASALDAQLCKLGSLDPIKTKGKILVCLR 426

Query: 424 GVTARVEKGYVVLQAGGVGMILVNDKTDGSGLISDSHILPATSLTYTDGLAVAQYINSTT 483
           G   RVEKG  V   GG+GM+L N    G+ L++D H+LPAT LT  D  AV++YI+ T 
Sbjct: 427 GQNGRVEKGRAVALGGGIGMVLENTYVTGNDLLADPHVLPATQLTSKDSFAVSRYISQTK 486

Query: 484 TPVALITPVETQLGVKPSPVMAGFSSRGPNPITDAMLKPDITGPGVNILASVTTDVTATK 543
            P+A ITP  T LG+KP+PVMA FSS+GP+ +   +LKPDIT PGV+++A+ T  V+ T 
Sbjct: 487 KPIAHITPSRTDLGLKPAPVMASFSSKGPSIVAPQILKPDITAPGVSVIAAYTGAVSPTN 546

Query: 544 FPFDTRRVPFNVESGTSMSCPHIAGVAGLLKTLYPTWSPAAIKSAIMTTAKTRDNSMHTL 603
             FD RR+ FN  SGTSMSCPHI+G+AGLLKT YP+WSPAAI+SAIMTTA   D+    +
Sbjct: 547 EQFDPRRLLFNAISGTSMSCPHISGIAGLLKTRYPSWSPAAIRSAIMTTATIMDDIPGPI 606

Query: 604 SDTVKAKATPFDYGAGHVNPNNAMDPGLVYDTTIDDYLNFLCARGYNAQTLMSFHSKPFV 663
            +    KATPF +GAGHV PN A++PGLVYD  I DYLNFLC+ GYNA  +  F    F 
Sbjct: 607 QNATNMKATPFSFGAGHVQPNLAVNPGLVYDLGIKDYLNFLCSLGYNASQISVFSGNNFT 666

Query: 664 CATP-FTLTDLNYPSISIPELKSGAPVTVNRRVKNVGTPGTYVARVKASSKISVTVEPST 723
           C++P  +L +LNYPSI++P L S + VTV+R VKNVG P  Y  +V     + V V+P++
Sbjct: 667 CSSPKISLVNLNYPSITVPNLTS-SKVTVSRTVKNVGRPSMYTVKVNNPQGVYVAVKPTS 726

Query: 724 LQFNSVGEEKAFKVVFEYKGTGQGKGYVFGTLIWSDNGKHNVRSPIVVKL 771
           L F  VGE+K FKV+         KGYVFG L+WSD  KH VRSPIVVKL
Sbjct: 727 LNFTKVGEQKTFKVILVKSKGNVAKGYVFGELVWSDK-KHRVRSPIVVKL 772

BLAST of IVF0023697 vs. TAIR 10
Match: AT5G59810.1 (Subtilase family protein )

HSP 1 Score: 798.5 bits (2061), Expect = 4.7e-231
Identity = 416/764 (54.45%), Postives = 523/764 (68.46%), Query Frame = 0

Query: 8   VSPLLLFFFLLQTSALPTKKSYIVYLGAHSFGPNPTNFDIEAATESHYDLLGSVVGNNLE 67
           +S LLL   L  + A   KKSYIVYLG+H+  P  ++  ++    SH   L S VG++  
Sbjct: 21  LSSLLLLVTLFFSPAFALKKSYIVYLGSHAHLPQISSAHLDGVAHSHRTFLASFVGSHEN 80

Query: 68  AKDSIKYSYNKYINGFAAVLDEQQATDLAKNPKVVSVFENKKRQLHTTRSWSFLGMENDE 127
           AK++I YSY ++INGFAA+LDE +A ++AK+P VVSVF NK R+LHTT SW+F+ +  + 
Sbjct: 81  AKEAIFYSYKRHINGFAAILDENEAAEIAKHPDVVSVFPNKGRKLHTTHSWNFMLLAKNG 140

Query: 128 GIPSNSIWKAARFGEDTIIGNLDSGVWPESKSFNDAGYGPVPSRWRGACEGGNNFQCNRK 187
            +  +S+W  A +GEDTII NLD+GVWPESKSF+D GYG VP+RW+G C    +  CNRK
Sbjct: 141 VVHKSSLWNKAGYGEDTIIANLDTGVWPESKSFSDEGYGAVPARWKGRCH--KDVPCNRK 200

Query: 188 LIGARYFHAGFVMGSGLINVSFNKTARDEDGHGSHTLSTAGGNFVPGASIFGYGNGTAKG 247
           LIGARYF+ G++  +GL + +  +T RD DGHGSHTLSTA GNFVPGA++FG GNGTA G
Sbjct: 201 LIGARYFNKGYLAYTGLPSNASYETCRDHDGHGSHTLSTAAGNFVPGANVFGIGNGTASG 260

Query: 248 GSPKARVAAYRVCWPPALFGGGCYDADILAGFEAAISDGVDVLSVSLGSEAQEFSHDSMS 307
           GSPKARVAAY+VCWPP + G  C+DADILA  EAAI DGVDVLS S+G +A ++  D ++
Sbjct: 261 GSPKARVAAYKVCWPP-VDGAECFDADILAAIEAAIEDGVDVLSASVGGDAGDYMSDGIA 320

Query: 308 IGAFHAVEQGIVVVCSAGNSGPGPKTVSNVSPWMFTVGASTIDRDFTSYATLGNKKQYKG 367
           IG+FHAV+ G+ VVCSAGNSGP   TVSNV+PW+ TVGAS++DR+F ++  L N + +KG
Sbjct: 321 IGSFHAVKNGVTVVCSAGNSGPKSGTVSNVAPWVITVGASSMDREFQAFVELKNGQSFKG 380

Query: 368 SSLSSSSLAGGKFYPLINAVDARNPNSTDSFAQKCETGSLDPAKVKGKIVVCLRGVTARV 427
           +SL S  L   K Y LI+A DA   N   + A  C+ GSLDP KVKGKI+VCLRG  ARV
Sbjct: 381 TSL-SKPLPEEKMYSLISAADANVANGNVTDALLCKKGSLDPKKVKGKILVCLRGDNARV 440

Query: 428 EKGYVVLQAGGVGMILVNDKTDGSGLISDSHILPATSLTYTDGLAVAQYINSTTTPVALI 487
           +KG     AG  GM+L NDK  G+ +ISD+H+LPA+ + Y DG  +  Y++ST  P   I
Sbjct: 441 DKGMQAAAAGAAGMVLCNDKASGNEIISDAHVLPASQIDYKDGETLFSYLSSTKDPKGYI 500

Query: 488 TPVETQLGVKPSPVMAGFSSRGPNPITDAMLKPDITGPGVNILASVTTDVTATKFPFDTR 547
                 L  KP+P MA FSSRGPN IT  +LKPDIT PGVNI+A+ T     T    D R
Sbjct: 501 KAPTATLNTKPAPFMASFSSRGPNTITPGILKPDITAPGVNIIAAFTEATGPTDLDSDNR 560

Query: 548 RVPFNVESGTSMSCPHIAGVAGLLKTLYPTWSPAAIKSAIMTTAKTRDNSMHTLSDTVKA 607
           R PFN ESGTSMSCPHI+GV GLLKTL+P WSPAAI+SAIMTT++TR+N    + D    
Sbjct: 561 RTPFNTESGTSMSCPHISGVVGLLKTLHPHWSPAAIRSAIMTTSRTRNNRRKPMVDESFK 620

Query: 608 KATPFDYGAGHVNPNNAMDPGLVYDTTIDDYLNFLCARGYNAQTLMSFHSKP-FVCATPF 667
           KA PF YG+GHV PN A  PGLVYD T  DYL+FLCA GYN   +  F   P + C    
Sbjct: 621 KANPFSYGSGHVQPNKAAHPGLVYDLTTGDYLDFLCAVGYNNTVVQLFAEDPQYTCRQGA 680

Query: 668 TLTDLNYPSISIPELKSGAPVTVNRRVKNVGTPGTYVARVKASSKISVTVEPSTLQFNSV 727
            L D NYPSI++P L     +TV R++KNVG P TY AR +    + V+VEP  L FN  
Sbjct: 681 NLLDFNYPSITVPNLTGS--ITVTRKLKNVGPPATYNARFREPLGVRVSVEPKQLTFNKT 740

Query: 728 GEEKAFKVVFEYKGTGQGKGYVFGTLIWSDNGKHNVRSPIVVKL 771
           GE K F++           GYVFG L W+D+  H VRSPIVV+L
Sbjct: 741 GEVKIFQMTLRPLPV-TPSGYVFGELTWTDS-HHYVRSPIVVQL 776

BLAST of IVF0023697 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 608.6 bits (1568), Expect = 6.9e-174
Identity = 340/766 (44.39%), Postives = 481/766 (62.79%), Query Frame = 0

Query: 11  LLLFFFLLQTSALPTKKSYIVYLGAHSFGPNPTNFDIEAATESHYDLLGSVVGNNLEAKD 70
           LL   F   +S+   + +YIV++        P++FD+ +   + YD     + ++ E   
Sbjct: 14  LLCLGFCHVSSSSSDQGTYIVHMAKSQM---PSSFDLHS---NWYDSSLRSISDSAE--- 73

Query: 71  SIKYSYNKYINGFAAVLDEQQATDLAKNPKVVSVFENKKRQLHTTRSWSFLGMENDEGIP 130
            + Y+Y   I+GF+  L +++A  L   P V+SV    + +LHTTR+  FLG++      
Sbjct: 74  -LLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEH---- 133

Query: 131 SNSIWKAARFGEDTIIGNLDSGVWPESKSFNDAGYGPVPSRWRGACEGGNNFQ---CNRK 190
           +  ++  A    D ++G LD+GVWPESKS++D G+GP+PS W+G CE G NF    CNRK
Sbjct: 134 TADLFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRK 193

Query: 191 LIGARYFHAGFVMGSGLINVS-FNKTARDEDGHGSHTLSTAGGNFVPGASIFGYGNGTAK 250
           LIGAR+F  G+    G I+ S  +++ RD+DGHG+HT STA G+ V GAS+ GY +GTA+
Sbjct: 194 LIGARFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTAR 253

Query: 251 GGSPKARVAAYRVCWPPALFGGGCYDADILAGFEAAISDGVDVLSVSLGSEAQEFSHDSM 310
           G +P+ARVA Y+VCW      GGC+ +DILA  + AI+D V+VLS+SLG    ++  D +
Sbjct: 254 GMAPRARVAVYKVCWL-----GGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGV 313

Query: 311 SIGAFHAVEQGIVVVCSAGNSGPGPKTVSNVSPWMFTVGASTIDRDFTSYATLGNKKQYK 370
           +IGAF A+E+GI+V CSAGN+GP   ++SNV+PW+ TVGA T+DRDF + A LGN K + 
Sbjct: 314 AIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFT 373

Query: 371 GSSLSSSSLAGGKFYPLINAVDARNPNSTDSFAQKCETGSLDPAKVKGKIVVCLRGVTAR 430
           G SL        K  P I A +A N  +       C TG+L P KVKGKIV+C RG+ AR
Sbjct: 374 GVSLFKGEALPDKLLPFIYAGNASNATN----GNLCMTGTLIPEKVKGKIVMCDRGINAR 433

Query: 431 VEKGYVVLQAGGVGMILVNDKTDGSGLISDSHILPATSLTYTDGLAVAQYINSTTTPVAL 490
           V+KG VV  AGGVGMIL N   +G  L++D+H+LPAT++    G  +  Y+ +   P A 
Sbjct: 434 VQKGDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTAS 493

Query: 491 ITPVETQLGVKPSPVMAGFSSRGPNPITDAMLKPDITGPGVNILASVTTDVTATKFPFDT 550
           I+ + T +GVKPSPV+A FSSRGPN IT  +LKPD+  PGVNILA+ T     T    D+
Sbjct: 494 ISILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDS 553

Query: 551 RRVPFNVESGTSMSCPHIAGVAGLLKTLYPTWSPAAIKSAIMTTA-KTRDNSMHTLSDTV 610
           RRV FN+ SGTSMSCPH++G+A LLK+++P WSPAAI+SA+MTTA KT  +    L    
Sbjct: 554 RRVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIAT 613

Query: 611 KAKATPFDYGAGHVNPNNAMDPGLVYDTTIDDYLNFLCARGYNAQTLMSFHSKPFVC--A 670
              +TPFD+GAGHV+P  A +PGL+YD T +DYL FLCA  Y +  + S   + + C  +
Sbjct: 614 GKPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPS 673

Query: 671 TPFTLTDLNYPSISIPELKSGAPVTVNRRVKNVGTPGTYVARVKA-SSKISVTVEPSTLQ 730
             +++ DLNYPS ++     GA     R V +VG  GTY  +V + ++ + ++VEP+ L 
Sbjct: 674 KSYSVADLNYPSFAVNVDGVGA-YKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLN 733

Query: 731 FNSVGEEKAFKVVFEYKGTGQGKGYVFGTLIWSDNGKHNVRSPIVV 769
           F    E+K++ V F    +       FG++ WSD GKH V SP+ +
Sbjct: 734 FKEANEKKSYTVTFTVDSSKPSGSNSFGSIEWSD-GKHVVGSPVAI 754

BLAST of IVF0023697 vs. TAIR 10
Match: AT5G51750.1 (subtilase 1.3 )

HSP 1 Score: 576.2 bits (1484), Expect = 3.8e-164
Identity = 336/786 (42.75%), Postives = 476/786 (60.56%), Query Frame = 0

Query: 4   FSYIVSPLLLFFFLLQTSA-LPTKKSYIVYLGAHSFGPNPTNFDIEAATESHYDLLGSVV 63
           F +I+  + L F   +T+  + TKK+Y++++   +     TN       + +   + SV 
Sbjct: 11  FLFIILSINLIFLQAETTTQISTKKTYVIHMDKSAMPLPYTNH-----LQWYSSKINSVT 70

Query: 64  GNNLEAKDS----IKYSYNKYINGFAAVLDEQQATDLAKNPKVVSVFENKKRQLHTTRSW 123
            +  + ++     I Y+Y    +G AA L +++A  L +   VV+V    + +LHTTRS 
Sbjct: 71  QHKSQEEEGNNNRILYTYQTAFHGLAAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSP 130

Query: 124 SFLGMENDEGIPSNSIWKAARFGEDTIIGNLDSGVWPESKSFNDAGYGPVPSRWRGACEG 183
           +FLG+E  E   S  +W       D ++G LD+G+WPES+SFND G  PVP+ WRGACE 
Sbjct: 131 TFLGLERQE---SERVWAERVTDHDVVVGVLDTGIWPESESFNDTGMSPVPATWRGACET 190

Query: 184 GNNF---QCNRKLIGARYFHAGFVMGSGLINVSFN-KTARDEDGHGSHTLSTAGGNFVPG 243
           G  F    CNRK++GAR F+ G+   +G I+     K+ RD DGHG+HT +T  G+ V G
Sbjct: 191 GKRFLKRNCNRKIVGARVFYRGYEAATGKIDEELEYKSPRDRDGHGTHTAATVAGSPVKG 250

Query: 244 ASIFGYGNGTAKGGSPKARVAAYRVCWPPALFGGGCYDADILAGFEAAISDGVDVLSVSL 303
           A++FG+  GTA+G + KARVAAY+VCW      GGC+ +DIL+  + A++DGV VLS+SL
Sbjct: 251 ANLFGFAYGTARGMAQKARVAAYKVCWV-----GGCFSSDILSAVDQAVADGVQVLSISL 310

Query: 304 GSEAQEFSHDSMSIGAFHAVEQGIVVVCSAGNSGPGPKTVSNVSPWMFTVGASTIDRDFT 363
           G     +S DS+SI  F A+E G+ V CSAGN GP P +++NVSPW+ TVGAST+DRDF 
Sbjct: 311 GGGVSTYSRDSLSIATFGAMEMGVFVSCSAGNGGPDPISLTNVSPWITTVGASTMDRDFP 370

Query: 364 SYATLGNKKQYKGSSL--SSSSLAGGKFYPLINAVDARNPNSTDSFAQKCETGSLDPAKV 423
           +   +G  + +KG SL    + L   K YPL+     RN +S D     C  G+LD   V
Sbjct: 371 ATVKIGTMRTFKGVSLYKGRTVLPKNKQYPLVYL--GRNASSPDP-TSFCLDGALDRRHV 430

Query: 424 KGKIVVCLRGVTARVEKGYVVLQAGGVGMILVNDKTDGSGLISDSHILPATSLTYTDGLA 483
            GKIV+C RGVT RV+KG VV +AGG+GM+L N  T+G  L++DSH+LPA ++   +G  
Sbjct: 431 AGKIVICDRGVTPRVQKGQVVKRAGGIGMVLTNTATNGEELVADSHMLPAVAVGEKEGKL 490

Query: 484 VAQYINSTTTPVALITPVETQLGVKPSPVMAGFSSRGPNPITDAMLKPDITGPGVNILAS 543
           + QY  ++    A +  + T++G+KPSPV+A FSSRGPN ++  +LKPD+  PGVNILA+
Sbjct: 491 IKQYAMTSKKATASLEILGTRIGIKPSPVVAAFSSRGPNFLSLEILKPDLLAPGVNILAA 550

Query: 544 VTTDVTATKFPFDTRRVPFNVESGTSMSCPHIAGVAGLLKTLYPTWSPAAIKSAIMTTAK 603
            T D+  +    D RRV FN+ SGTSMSCPH++GVA L+K+ +P WSPAAIKSA+MTTA 
Sbjct: 551 WTGDMAPSSLSSDPRRVKFNILSGTSMSCPHVSGVAALIKSRHPDWSPAAIKSALMTTAY 610

Query: 604 TRDNSMHTLSD-TVKAKATPFDYGAGHVNPNNAMDPGLVYDTTIDDYLNFLCARGYNAQT 663
             DN    L+D +  A ++P+D+GAGH++P  A DPGLVYD    +Y  FLC +  +   
Sbjct: 611 VHDNMFKPLTDASGAAPSSPYDHGAGHIDPLRATDPGLVYDIGPQEYFEFLCTQDLSPSQ 670

Query: 664 LMSF--HS----KPFVCATPFTLTDLNYPSIS--IPELKSGAPVTVNRRVKNVGTP-GTY 723
           L  F  HS    K  +   P    +LNYP+IS   PE      +T+ R V NVG    +Y
Sbjct: 671 LKVFTKHSNRTCKHTLAKNP---GNLNYPAISALFPENTHVKAMTLRRTVTNVGPHISSY 730

Query: 724 VARVKASSKISVTVEPSTLQFNSVGEEKAFKVVFEYKGTGQGKGYVFGTLIWSDNGKHNV 769
              V      SVTV+P TL F S  ++ ++ V F  +   + K   FG L+W     H V
Sbjct: 731 KVSVSPFKGASVTVQPKTLNFTSKHQKLSYTVTFRTR--FRMKRPEFGGLVWKST-THKV 774

BLAST of IVF0023697 vs. TAIR 10
Match: AT3G14067.1 (Subtilase family protein )

HSP 1 Score: 574.3 bits (1479), Expect = 1.4e-163
Identity = 341/787 (43.33%), Postives = 478/787 (60.74%), Query Frame = 0

Query: 6   YIVSPLLLFFFLLQTSALPTKKSYIVYLGAHSFGPNPTNFDIEAATESHYDLLGSVVGNN 65
           + V PLLL FF   +S+    +SYIV++        P+ F   +    H  LL S+  + 
Sbjct: 10  FFVFPLLLCFFSPSSSSSDGLESYIVHV---QRSHKPSLF--SSHNNWHVSLLRSLPSSP 69

Query: 66  LEAKDSIKYSYNKYINGFAAVLDEQQATDLAKNPKVVSVFENKKRQLHTTRSWSFLGMEN 125
             A  ++ YSY++ ++GF+A L   Q   L ++P V+SV  ++ R++HTT + +FLG   
Sbjct: 70  QPA--TLLYSYSRAVHGFSARLSPIQTAALRRHPSVISVIPDQAREIHTTHTPAFLGFSQ 129

Query: 126 DEGIPSNSIWKAARFGEDTIIGNLDSGVWPESKSFNDAGYGPVPSRWRGACEGGNNF--- 185
           + G     +W  + +GED I+G LD+G+WPE  SF+D+G GP+PS W+G CE G +F   
Sbjct: 130 NSG-----LWSNSNYGEDVIVGVLDTGIWPEHPSFSDSGLGPIPSTWKGECEIGPDFPAS 189

Query: 186 QCNRKLIGARYFHAGFVM---GSGLINVSFNKTARDEDGHGSHTLSTAGGNFVPGASIFG 245
            CNRKLIGAR F+ G++    G+       +++ RD +GHG+HT STA G+ V  AS++ 
Sbjct: 190 SCNRKLIGARAFYRGYLTQRNGTKKHAAKESRSPRDTEGHGTHTASTAAGSVVANASLYQ 249

Query: 246 YGNGTAKGGSPKARVAAYRVCWPPALFGGGCYDADILAGFEAAISDGVDVLSVSLGS--E 305
           Y  GTA G + KAR+AAY++CW      GGCYD+DILA  + A++DGV V+S+S+G+   
Sbjct: 250 YARGTATGMASKARIAAYKICWT-----GGCYDSDILAAMDQAVADGVHVISLSVGASGS 309

Query: 306 AQEFSHDSMSIGAFHAVEQGIVVVCSAGNSGPGPKTVSNVSPWMFTVGASTIDRDFTSYA 365
           A E+  DS++IGAF A   GIVV CSAGNSGP P+T +N++PW+ TVGAST+DR+F + A
Sbjct: 310 APEYHTDSIAIGAFGATRHGIVVSCSAGNSGPNPETATNIAPWILTVGASTVDREFAANA 369

Query: 366 TLGNKKQYKGSSLSSSSLAGGKFYPLINAVDARNPNSTDSFAQKCETGSLDPAKVKGKIV 425
             G+ K + G+SL +          L+         S D  ++ C  G L+ + V+GKIV
Sbjct: 370 ITGDGKVFTGTSLYAGESLPDSQLSLV--------YSGDCGSRLCYPGKLNSSLVEGKIV 429

Query: 426 VCLRGVTARVEKGYVVLQAGGVGMILVNDKTDGSGLISDSHILPATSLTYTDGLAVAQYI 485
           +C RG  ARVEKG  V  AGG GMIL N    G  L +DSH++PAT +    G  +  YI
Sbjct: 430 LCDRGGNARVEKGSAVKLAGGAGMILANTAESGEELTADSHLVPATMVGAKAGDQIRDYI 489

Query: 486 NSTTTPVALITPVETQLGVK-PSPVMAGFSSRGPNPITDAMLKPDITGPGVNILASVTTD 545
            ++ +P A I+ + T +G   PSP +A FSSRGPN +T  +LKPD+  PGVNILA  T  
Sbjct: 490 KTSDSPTAKISFLGTLIGPSPPSPRVAAFSSRGPNHLTPVILKPDVIAPGVNILAGWTGM 549

Query: 546 VTATKFPFDTRRVPFNVESGTSMSCPHIAGVAGLLKTLYPTWSPAAIKSAIMTTAKTRDN 605
           V  T    D RRV FN+ SGTSMSCPH++G+A LL+  +P WSPAAIKSA++TTA   +N
Sbjct: 550 VGPTDLDIDPRRVQFNIISGTSMSCPHVSGLAALLRKAHPDWSPAAIKSALVTTAYDVEN 609

Query: 606 SMHTLSDTVKAKAT-PFDYGAGHVNPNNAMDPGLVYDTTIDDYLNFLCARGYNAQTLMSF 665
           S   + D    K++  F +GAGHV+PN A++PGLVYD  + +Y+ FLCA GY    ++ F
Sbjct: 610 SGEPIEDLATGKSSNSFIHGAGHVDPNKALNPGLVYDIEVKEYVAFLCAVGYEFPGILVF 669

Query: 666 HSKPFV---CATP--FTLTDLNYPSISIPELKSGAPVTVNRRVKNVGT--PGTYVARVKA 725
              P +   C T    T  DLNYPS S+    +G  V   R VKNVG+     Y   VK+
Sbjct: 670 LQDPTLYDACETSKLRTAGDLNYPSFSVVFASTGEVVKYKRVVKNVGSNVDAVYEVGVKS 729

Query: 726 SSKISVTVEPSTLQFN---SVGE-EKAFKVVFEYKGTGQGKGYVFGTLIWSDNGKHNVRS 772
            + + + V PS L F+   SV E E  FK V    G G   G+ FG++ W+D G+H V+S
Sbjct: 730 PANVEIDVSPSKLAFSKEKSVLEYEVTFKSVVLGGGVGSVPGHEFGSIEWTD-GEHVVKS 770

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9ZSP56.0e-24757.01Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=... [more]
F4JXC56.6e-23054.45Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 S... [more]
I1N4624.1e-20350.13Subtilisin-like protease Glyma18g48580 OS=Glycine max OX=3847 GN=Glyma18g48580 P... [more]
O653519.8e-17344.39Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 S... [more]
Q9FLI45.4e-16342.75Subtilisin-like protease SBT1.3 OS=Arabidopsis thaliana OX=3702 GN=SBT1.3 PE=2 S... [more]
Match NameE-valueIdentityDescription
A0A1S3BLG80.0e+0099.61subtilisin-like protease SBT5.3 OS=Cucumis melo OX=3656 GN=LOC103491134 PE=3 SV=... [more]
A0A5D3D7320.0e+00100.00Subtilisin-like protease SBT5.3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_... [more]
A0A0A0L6010.0e+0084.27Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G045060 PE=3 SV=1[more]
A0A6J1GDW20.0e+0071.08subtilisin-like protease SBT5.3 OS=Cucurbita moschata OX=3662 GN=LOC111453052 PE... [more]
A0A6J1KUL80.0e+0070.76uncharacterized protein LOC111498820 OS=Cucurbita maxima OX=3661 GN=LOC111498820... [more]
Match NameE-valueIdentityDescription
XP_008449184.10.099.61PREDICTED: subtilisin-like protease SBT5.3 [Cucumis melo][more]
TYK19386.10.0100.00subtilisin-like protease SBT5.3 [Cucumis melo var. makuwa][more]
XP_031738719.10.084.16uncharacterized protein LOC101212014 [Cucumis sativus][more]
KAE8650093.10.082.72hypothetical protein Csa_009608 [Cucumis sativus][more]
XP_038905686.10.078.78subtilisin-like protease SBT5.3 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT2G04160.14.3e-24857.01Subtilisin-like serine endopeptidase family protein [more]
AT5G59810.14.7e-23154.45Subtilase family protein [more]
AT5G67360.16.9e-17444.39Subtilase family protein [more]
AT5G51750.13.8e-16442.75subtilase 1.3 [more]
AT3G14067.11.4e-16343.33Subtilase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR015500Peptidase S8, subtilisin-relatedPRINTSPR00723SUBTILISINcoord: 141..160
score: 26.02
coord: 555..571
score: 55.47
coord: 215..228
score: 34.04
IPR003137PA domainPFAMPF02225PAcoord: 400..472
e-value: 1.3E-10
score: 41.2
IPR000209Peptidase S8/S53 domainPFAMPF00082Peptidase_S8coord: 142..603
e-value: 2.8E-39
score: 135.8
NoneNo IPR availableGENE3D2.60.40.2310coord: 642..770
e-value: 7.7E-35
score: 121.5
NoneNo IPR availableGENE3D3.50.30.30coord: 350..494
e-value: 9.6E-185
score: 616.8
NoneNo IPR availablePANTHERPTHR10795:SF662SUBTILISIN-LIKE PROTEASE SBT5.4coord: 27..771
NoneNo IPR availablePROSITEPS51892SUBTILASEcoord: 117..625
score: 27.982317
NoneNo IPR availableCDDcd02120PA_subtilisin_likecoord: 356..487
e-value: 2.30498E-33
score: 122.522
NoneNo IPR availableSUPERFAMILY52025PA domaincoord: 409..470
IPR010259Peptidase S8 propeptide/proteinase inhibitor I9PFAMPF05922Inhibitor_I9coord: 28..113
e-value: 4.9E-16
score: 59.2
IPR036852Peptidase S8/S53 domain superfamilyGENE3D3.40.50.200Peptidase S8/S53 domaincoord: 143..638
e-value: 9.6E-185
score: 616.8
IPR036852Peptidase S8/S53 domain superfamilySUPERFAMILY52743Subtilisin-likecoord: 112..629
IPR041469Subtilisin-like protease, fibronectin type-III domainPFAMPF17766fn3_6coord: 670..768
e-value: 2.6E-28
score: 98.1
IPR037045Peptidase S8 propeptide/proteinase inhibitor I9 superfamilyGENE3D3.30.70.80Peptidase S8 propeptide/proteinase inhibitor I9coord: 18..113
e-value: 6.4E-24
score: 86.2
IPR045051Subtilisin-like proteasePANTHERPTHR10795PROPROTEIN CONVERTASE SUBTILISIN/KEXINcoord: 27..771
IPR023828Peptidase S8, subtilisin, Ser-active sitePROSITEPS00138SUBTILASE_SERcoord: 556..566
IPR034197Cucumisin-like catalytic domainCDDcd04852Peptidases_S8_3coord: 111..591
e-value: 6.51079E-134
score: 397.354

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
IVF0023697.1IVF0023697.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
biological_process GO:0009610 response to symbiotic fungus
molecular_function GO:0004252 serine-type endopeptidase activity
molecular_function GO:0008236 serine-type peptidase activity