IVF0022879 (gene) Melon (IVF77) v1

BLAST of IVF0022879 vs. ExPASy Swiss-Prot
Match: Q9M0S5 (Isoamylase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ISA3 PE=1 SV=2)

HSP 1 Score: 1010.0 bits (2610), Expect = 1.5e-293
Identity = 491/735 (66.80%), Postives = 565/735 (76.87%), Query Frame = 0

Query: 64  TVSAFSRRDQDRVLEQEAPENLDTGPMLKIFPGQAFPLGVSEVDNGINFAIFSQHATSVT 123
           T+    RR  +RV+E+EA    +T  + K+  G+  PLGVS+VD GINFA+FSQ+ATSVT
Sbjct: 57  TLKVTCRRAHERVVEEEASTMTET-KLFKVSSGEVSPLGVSQVDKGINFALFSQNATSVT 116

Query: 124 ICLSL-----DGRIDDGMLEFKLDPDDNRTGDIWHICIQDLQRKNVLYGYRIDGPQGWHH 183
           +CLSL     D   DDGM+E  LDP  N+TGD WHIC++DL   NVLYGYR+DGP  W  
Sbjct: 117 LCLSLSQSGKDDTDDDGMIELVLDPSVNKTGDTWHICVEDLPLNNVLYGYRVDGPGEWQQ 176

Query: 184 GHRYDDGTMLLDPYAKFVEGRRFFG-GENKSSGFLGTYDFESLPFDWGNDYKLPNIPEKD 243
           GHR+D   +LLDPYAK V+G   FG    K + F GTYDFES PFDWG+DYK PNIPEKD
Sbjct: 177 GHRFDRSILLLDPYAKLVKGHSSFGDSSQKFAQFYGTYDFESSPFDWGDDYKFPNIPEKD 236

Query: 244 LVIYEMNVRGFTADESSGLPSSTRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQR 303
           LVIYEMNVR FTADESSG+  +  GSYLG IEKIPHL +LG+NAVELLPVFEFDELE QR
Sbjct: 237 LVIYEMNVRAFTADESSGMDPAIGGSYLGFIEKIPHLQDLGINAVELLPVFEFDELELQR 296

Query: 304 HPNPRDHMINTWGYSTINFFAPMSRYASAGGGPLNASQEFKQMVKALHAAGIEVILDVVY 363
             NPRDHM+NTWGYST+NFFAPMSRYAS  G P+ AS+EFK+MVKALH+AGIEVILDVVY
Sbjct: 297 RSNPRDHMVNTWGYSTVNFFAPMSRYASGEGDPIKASKEFKEMVKALHSAGIEVILDVVY 356

Query: 364 NHTNEGDDVNPYTTSFRGIDNKVYYMLDLKNNGQYFNFSGC-----ATHPLVLQVYSGSL 423
           NHTNE DD  PYTTSFRGIDNKVYYMLD   N Q  NFSGC       HP+V+++   S 
Sbjct: 357 NHTNEADDKYPYTTSFRGIDNKVYYMLD--PNNQLLNFSGCGNTLNCNHPVVMELILDS- 416

Query: 424 MISCNSTLIYYSTILNFRVVEYHVDGFRFDLASVLCRGTDGAPTYIRQVDMARDLNLLLF 483
                         L   V EYHVDGFRFDLASVLCR TDG+P               L 
Sbjct: 417 --------------LRHWVTEYHVDGFRFDLASVLCRATDGSPLSAPP----------LI 476

Query: 484 QAISKDAILSRCKIIAEPWDCGGLIL---------WAEWNGIYRDDIRKFIKGDCGMKGS 543
           +AI+KD++LSRCKIIAEPWDCGGL L         WAEWNG+YRDD+R+FIKGD GMKGS
Sbjct: 477 RAIAKDSVLSRCKIIAEPWDCGGLYLVGKFPNWDRWAEWNGMYRDDVRRFIKGDSGMKGS 536

Query: 544 FATRVAGSSDLYNVNKRKPYHGINFVIAHDGFTLRDLVSYNVKHNDANGEGGNDGCNDNF 603
           FATRV+GSSDLY VN+RKPYHG+NFVIAHDGFTLRDLVSYN KHN+ANGEGGNDGCNDN 
Sbjct: 537 FATRVSGSSDLYQVNQRKPYHGVNFVIAHDGFTLRDLVSYNFKHNEANGEGGNDGCNDNH 596

Query: 604 SWNCGFEGETEDTSIKALRSRQMKNFHLALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDN 663
           SWNCGFEGET D  IK+LR+RQMKNFHLALM SQGTPMMLMGDEYGHTRYGNNNSYGHD 
Sbjct: 597 SWNCGFEGETGDAHIKSLRTRQMKNFHLALMISQGTPMMLMGDEYGHTRYGNNNSYGHDT 656

Query: 664 ALNHFLWEQLEARKRDHFRFFSEVIKFRRKHPHLFKDSF--QNDITWHESNWDNPESKFL 723
           +LN+F W++L+A+K++HFRFFSEVIKFR  H  L  ++F  Q +ITWHE NWDN ESKFL
Sbjct: 657 SLNNFQWKELDAKKQNHFRFFSEVIKFRHSHHVLKHENFLTQGEITWHEDNWDNSESKFL 716

Query: 724 AYTLHDD-NGEDVYLAFNAHDYFVNVSLPSPPTNRRWFRVVDTNLESPHDFVLDGIPGVG 776
           A+TLHD   G D+Y+AFNAHDYFV   +P PP  ++WFRV DTNLESP DFV +G+ GV 
Sbjct: 717 AFTLHDGIGGRDIYVAFNAHDYFVKALIPQPPPGKQWFRVADTNLESPDDFVREGVAGVA 763

BLAST of IVF0022879 vs. ExPASy Swiss-Prot
Match: B9G434 (Isoamylase 3, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISA3 PE=2 SV=1)

HSP 1 Score: 991.9 bits (2563), Expect = 4.1e-288
Identity = 479/710 (67.46%), Postives = 556/710 (78.31%), Query Frame = 0

Query: 86  DTGPMLKIFPGQAFPLGVSEVDNGINFAIFSQHATSVTICLSLDGRIDD-----GMLEFK 145
           DT    K   G+AFPLGVS+V+ G+NFA+FSQHA+SV +CL L GR  +      ++EF 
Sbjct: 93  DTEMPFKYSSGKAFPLGVSQVEGGLNFALFSQHASSVILCLKLPGRGTEDEKGADVVEFV 152

Query: 146 LDPDDNRTGDIWHICIQDLQRKNVLYGYRIDGPQGWHHGHRYDDGTMLLDPYAKFVEGRR 205
           LD   N+TGDIWH+ ++ L    VLYGYR+ GPQGW  GHR+D  T+LLDPYAK V GR+
Sbjct: 153 LDQQKNKTGDIWHVIVEGLPASGVLYGYRVGGPQGWDQGHRFDSSTVLLDPYAKLVSGRK 212

Query: 206 FFG-GENKSSGFLGTYDFESLPFDWGNDYKLPNIPEKDLVIYEMNVRGFTADESSGLPSS 265
           +FG  E KSS   GTYDF+S PFDWG+DY+LPN+PE DLVIYEMNVR FTADESSGL S+
Sbjct: 213 YFGVAEEKSSQHFGTYDFDSSPFDWGDDYRLPNLPEADLVIYEMNVRAFTADESSGLDST 272

Query: 266 TRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHPNPRDHMINTWGYSTINFFAP 325
           +RGSYLG+I+KIPHLLELGVNAVELLPVFE+DELEF+R+PNPRDHM+NTWGYSTINFFAP
Sbjct: 273 SRGSYLGLIDKIPHLLELGVNAVELLPVFEYDELEFKRYPNPRDHMVNTWGYSTINFFAP 332

Query: 326 MSRYASAGGGPLNASQEFKQMVKALHAAGIEVILDVVYNHTNEGDDVNPYTTSFRGIDNK 385
           MSRYASAGGGP+ AS+E KQMVK LH AGIEVILDVVYNHTNE DD +PY TSFRGIDNK
Sbjct: 333 MSRYASAGGGPVAASKELKQMVKELHKAGIEVILDVVYNHTNEADDAHPYMTSFRGIDNK 392

Query: 386 VYYMLDLKNNGQYFNFSGCATHPLVLQVYSGSLMISCNSTLIYYSTILNFR--VVEYHVD 445
           VYYMLDL  N +  NFSGC               ++CN  ++    + + R  V EYH+D
Sbjct: 393 VYYMLDLNKNAELLNFSGCGN------------TLNCNHPVVKELILDSLRHWVEEYHID 452

Query: 446 GFRFDLASVLCRGTDGAPTYIRQVDMARDLNLLLFQAISKDAILSRCKIIAEPWDCGGLI 505
           GFRFDLASVLCRG DG P     +D        L + I+KDA+LSRCKIIAEPWDCGGL 
Sbjct: 453 GFRFDLASVLCRGPDGCP-----LDAPP-----LIKEIAKDAVLSRCKIIAEPWDCGGLY 512

Query: 506 L---------WAEWNGIYRDDIRKFIKGDCGMKGSFATRVAGSSDLYNVNKRKPYHGINF 565
           L         WAEWNG YRDD+R+FIKGD GMKG FATRV+GS+DLY VN+RKPYHG+NF
Sbjct: 513 LVGRFPNWDRWAEWNGKYRDDLRRFIKGDPGMKGVFATRVSGSADLYQVNERKPYHGVNF 572

Query: 566 VIAHDGFTLRDLVSYNVKHNDANGEGGNDGCNDNFSWNCGFEGETEDTSIKALRSRQMKN 625
           VIAHDGFTL DLVSYN+KHNDANGEGG DGCNDNFSWNCG EGET D ++ +LRSRQMKN
Sbjct: 573 VIAHDGFTLCDLVSYNLKHNDANGEGGCDGCNDNFSWNCGVEGETNDLNVLSLRSRQMKN 632

Query: 626 FHLALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDNALNHFLWEQLEARKRDHFRFFSEVI 685
           FH+ALM SQGTPMMLMGDEYGHTRYGNNNSYGHD  +N+F WEQLE R+  HFRFFSE+I
Sbjct: 633 FHVALMISQGTPMMLMGDEYGHTRYGNNNSYGHDTCINNFQWEQLEQRRDGHFRFFSEMI 692

Query: 686 KFRRKHPHLFKDSF--QNDITWHESNWDNPESKFLAYTLHDDN-GEDVYLAFNAHDYFVN 745
           KFR  +P L +D F  +ND+TWHE  W+N ESKFLA+T+HD N G D+YLAFNAHDYFV+
Sbjct: 693 KFRHSNPILRRDRFLNKNDVTWHEDCWENQESKFLAFTVHDHNSGGDIYLAFNAHDYFVD 752

Query: 746 VSLPSPPTNRRWFRVVDTNLESPHDFVLDGIPGVGSSYNLAPYSSILLKA 776
             +P PP ++ W RVVDTNLESP+D V +G+P  G  Y +APYSSILLKA
Sbjct: 753 AVIPPPPHHKCWNRVVDTNLESPNDIVPEGVPFTGPKYRIAPYSSILLKA 780

BLAST of IVF0022879 vs. ExPASy Swiss-Prot
Match: O04196 (Isoamylase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ISA1 PE=1 SV=1)

HSP 1 Score: 542.3 bits (1396), Expect = 8.7e-153
Identity = 331/765 (43.27%), Postives = 435/765 (56.86%), Query Frame = 0

Query: 64  TVSAFSRR--DQDRVLEQEAPENLDTGPMLKIFPGQAFPLGVSEVDNGINFAIFSQHATS 123
           ++SA  RR  + + +   E P   D      I  G   P G +  D+G+NF+++S ++ S
Sbjct: 41  SISAKDRRSNEAENIAVVEKPLKSD---RFFISDGLPSPFGPTVRDDGVNFSVYSTNSVS 100

Query: 124 VTICL-SL-DGRIDDGMLEFKLDPDDNRTGDIWHICIQDLQRKNVLYGYRIDGPQGWHHG 183
            TICL SL D R +    E +LDP  NRTG +WH+ ++    K++LYGYR DG      G
Sbjct: 101 ATICLISLSDLRQNKVTEEIQLDPSRNRTGHVWHVFLRG-DFKDMLYGYRFDGKFSPEEG 160

Query: 184 HRYDDGTMLLDPYAKFVEGRRFFG----GENKSSGFLGTYDFESLPFDWGNDYKLPNIPE 243
           H YD   +LLDPYAK +  R  FG     +N               FDW  D  L  +P+
Sbjct: 161 HYYDSSNILLDPYAKAIISRDEFGVLGPDDNCWPQMACMVPTREEEFDWEGDMHL-KLPQ 220

Query: 244 KDLVIYEMNVRGFTADESSGLPSSTRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEF 303
           KDLVIYEM+VRGFT  ESS +     G+Y GV EK+ HL ELG+N +EL+P  EF+ELE+
Sbjct: 221 KDLVIYEMHVRGFTRHESSKI--EFPGTYQGVAEKLDHLKELGINCIELMPCHEFNELEY 280

Query: 304 QRHPN-PRDHMINTWGYSTINFFAPMSRYASAGGGPL--NASQEFKQMVKALHAAGIEVI 363
             +     DH +N WGYSTI FF+PM RYASA        A  EFK +VK  H  GIEVI
Sbjct: 281 YSYNTILGDHRVNFWGYSTIGFFSPMIRYASASSNNFAGRAINEFKILVKEAHKRGIEVI 340

Query: 364 LDVVYNHTNEGDDVNPYTTSFRGIDNKVYYMLDLKNNGQYFNFSGC-----ATHPLVLQV 423
           +DVV NHT EG++  P   SFRG+DN VYYML  K  G+++N+SGC       HP+V Q 
Sbjct: 341 MDVVLNHTAEGNEKGP-IFSFRGVDNSVYYMLAPK--GEFYNYSGCGNTFNCNHPVVRQ- 400

Query: 424 YSGSLMISCNSTLIYYSTILNFRVVEYHVDGFRFDLASVLCRGT---DGAPTYIRQVDMA 483
                ++ C          L + V E HVDGFRFDL S++ R +   D A  Y    D+ 
Sbjct: 401 ----FILDC----------LRYWVTEMHVDGFRFDLGSIMSRSSSLWDAANVY--GADVE 460

Query: 484 RDLNLL--------LFQAISKDAILSRCKIIAEPWDCGGLI---------LWAEWNGIYR 543
            DL           +   IS D IL   K+IAE WD GGL          +W+EWNG +R
Sbjct: 461 GDLLTTGTPISCPPVIDMISNDPILRGVKLIAEAWDAGGLYQVGMFPHWGIWSEWNGKFR 520

Query: 544 DDIRKFIKGDCGMKGSFATRVAGSSDLYNVNKRKPYHGINFVIAHDGFTLRDLVSYNVKH 603
           D +R+FIKG  G  G+FA  + GS +LY    RKP+H INF+ AHDGFTL DLV+YN K+
Sbjct: 521 DVVRQFIKGTDGFSGAFAECLCGSPNLYQ-GGRKPWHSINFICAHDGFTLADLVTYNNKN 580

Query: 604 NDANGEGGNDGCNDNFSWNCGFEGETEDTSIKALRSRQMKNFHLALMTSQGTPMMLMGDE 663
           N ANGE  NDG N N+SWNCG EG+    S+K LR RQM+NF ++LM SQG PM+ MGDE
Sbjct: 581 NLANGEENNDGENHNYSWNCGEEGDFASISVKRLRKRQMRNFFVSLMVSQGVPMIYMGDE 640

Query: 664 YGHTRYGNNNSYGHDNALNHFLWEQLEARKRDHFRFFSEVIKFRRKHPHLFKDSF--QND 723
           YGHT+ GNNN+Y HDN +N+F W++ E    D FRF   +IKFR +   L  + F     
Sbjct: 641 YGHTKGGNNNTYCHDNYMNYFRWDKKEEAHSDFFRFCRILIKFRDECESLGLNDFPTAKR 700

Query: 724 ITWHE-----SNWDNPESKFLAYTLHDDNGEDVYLAFNAHDYFVNVSLPSPPTNRRWFRV 774
           + WH       NW +  S+F+A++L D   +++Y+AFN       VSLP+ P   RW   
Sbjct: 701 LQWHGLAPEIPNW-SETSRFVAFSLVDSVKKEIYVAFNTSHLATLVSLPNRP-GYRWEPF 760

BLAST of IVF0022879 vs. ExPASy Swiss-Prot
Match: D0TZF0 (Isoamylase 1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISA1 PE=1 SV=1)

HSP 1 Score: 533.5 bits (1373), Expect = 4.0e-150
Identity = 318/755 (42.12%), Postives = 422/755 (55.89%), Query Frame = 0

Query: 72  DQDRVLEQEAPENLDTGPMLKIFPGQAFPLGVSEVDNGINFAIFSQHATSVTICLSL--D 131
           +++ V     PE    G   ++  G   PLG + +D G+NFA++S  A++ ++CL    D
Sbjct: 75  EEEEVEAVVMPERYALGGACRVLAGMPAPLGATALDGGVNFAVYSAGASAASLCLFTPDD 134

Query: 132 GRIDDGMLEFKLDPDDNRTGDIWHICIQDLQRKNVLYGYRIDGPQGWHHGHRYDDGTMLL 191
              D+   E  LDP  NRTG++WH+ I+  +  N+LYGYR DG    H G  +D   +++
Sbjct: 135 LEADEVTEEVPLDPLFNRTGNVWHVFIEG-ELHNMLYGYRFDGMFAPHCGQYFDVSNVVV 194

Query: 192 DPYAKFVEGRRFFG----GENKSSGFLGTYDFESLPFDWGNDYKLPNIPEKDLVIYEMNV 251
           DPYAK V  R  +G    G +      G        FDW  D  L   P+KDLVIYEM++
Sbjct: 195 DPYAKAVISRGEYGVPGPGGDCWPQMAGMIPLPYSTFDWQGDLPL-RYPQKDLVIYEMHL 254

Query: 252 RGFTADESSGLPSSTRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHPNPRDHM 311
           RGFT   SS +     G+Y+G I K+ +L ELGVN VEL+P  EF+ELE+    +     
Sbjct: 255 RGFTKHSSSNVEHP--GTYIGAISKLDYLKELGVNCVELMPCHEFNELEYFSCSS----K 314

Query: 312 INTWGYSTINFFAPMSRYASAG--GGPLNASQEFKQMVKALHAAGIEVILDVVYNHTNEG 371
           +N WGYSTINFF+PM RY+S G      +A  EFK  V+  H  GIEVI+DVV+NHT EG
Sbjct: 315 MNFWGYSTINFFSPMIRYSSGGIRNCGRDAINEFKTFVREAHKRGIEVIMDVVFNHTAEG 374

Query: 372 DDVNPYTTSFRGIDNKVYYMLDLKNNGQYFNFSGC-----ATHPLVLQVYSGSLMISCNS 431
           ++  P   SFRGIDN  YYML  K  G+++N+SGC       HP+V +      ++ C  
Sbjct: 375 NEKGP-ILSFRGIDNSTYYMLAPK--GEFYNYSGCGNTFNCNHPVVRE-----FIVDC-- 434

Query: 432 TLIYYSTILNFRVVEYHVDGFRFDLASVLCRGTD--------GAPTYIRQVDMARDLNLL 491
                   L + V E HVDGFRFDLAS++ RG          G+P    + DM      L
Sbjct: 435 --------LRYWVTEMHVDGFRFDLASIMTRGCSLWDPVNVYGSPV---EGDMTTTGTPL 494

Query: 492 ----LFQAISKDAILSRCKIIAEPWDCGGLI---------LWAEWNGIYRDDIRKFIKGD 551
               L   IS D IL   K+IAE WD GGL          +W+EWNG YRD +R+FIKG 
Sbjct: 495 ATPPLIDMISNDPILGDVKLIAEAWDAGGLYQVGQFPHWKIWSEWNGKYRDIVRQFIKGT 554

Query: 552 CGMKGSFATRVAGSSDLYNVNKRKPYHGINFVIAHDGFTLRDLVSYNVKHNDANGEGGND 611
            G  G FA  + GS  LY    RKP+H INFV AHDGFTL DLV+YN K+N +NGE   D
Sbjct: 555 DGFAGGFAECLCGSPHLYQAGGRKPWHSINFVCAHDGFTLADLVTYNKKYNSSNGEDNRD 614

Query: 612 GCNDNFSWNCGFEGETEDTSIKALRSRQMKNFHLALMTSQGTPMMLMGDEYGHTRYGNNN 671
           G N N SWNCG EGE    S+K LR RQM+NF ++LM SQG PM  MGDEYGHT+ GNNN
Sbjct: 615 GENHNLSWNCGEEGEFAGLSVKRLRKRQMRNFFVSLMVSQGVPMFYMGDEYGHTKGGNNN 674

Query: 672 SYGHDNALNHFLWEQLEARKRDHFRFFSEVIKFRRKHPHLFKDSF--QNDITWHESNWDN 731
           +Y HD+ +N+F W++ E    D  RF S + KFR++   L    F     + WH      
Sbjct: 675 TYCHDHYVNYFRWDKKE-ESSDLQRFCSLMTKFRKQCESLGLADFPTAQRLHWHGHQPGK 734

Query: 732 PE----SKFLAYTLHDDNGEDVYLAFNAHDYFVNVSLPSPPTNRRWFRVVDTNLESPHDF 775
           P+    S+F+A++  D+   ++Y+AFNA      V LP  P   RW  +VDT   +P+DF
Sbjct: 735 PDWSETSRFVAFSTKDETKGEIYVAFNASHLPAVVGLPERP-GYRWEPLVDTGKPAPYDF 794

BLAST of IVF0022879 vs. ExPASy Swiss-Prot
Match: P0A4Y5 (Glycogen operon protein GlgX homolog OS=Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97) OX=233413 GN=glgX PE=3 SV=1)

HSP 1 Score: 426.8 bits (1096), Expect = 5.3e-118
Identity = 291/729 (39.92%), Postives = 375/729 (51.44%), Query Frame = 0

Query: 81  APENLDTGPML-KIFPGQAFPLGVSEVDNGINFAIFSQHATSVTICLSLDGRIDDGMLEF 140
           A E+  TGP L  ++PG A+PLG +    G NF++FS+ A  V +CL     ID+  +E 
Sbjct: 6   AGESDGTGPALPTVWPGNAYPLGATYDGAGTNFSLFSEIAEKVELCL-----IDEDGVES 65

Query: 141 KLDPDDNRTGDIWHICIQDLQRKNVLYGYRIDGPQGWHHGHRYDDGTMLLDPYAKFVEGR 200
           ++ P D   G +WH  + ++      YG+R+ GP     GHR D   +LLDPY K   G 
Sbjct: 66  RI-PLDEVDGYVWHAYLPNI-TPGQRYGFRVHGPFDPAAGHRCDPSKLLLDPYGKSFHG- 125

Query: 201 RFFGGENKSSGFLGTYDFESLP------------------FDWGNDYKLPNIPEKDLVIY 260
            F  G+   S  +   D +S P                  FDW  D + P  P  + VIY
Sbjct: 126 DFTFGQALYSYDVNAVDPDSTPPMVDSLGHTMTSVVINPFFDWAYD-RSPRTPYHETVIY 185

Query: 261 EMNVRGFTADESSGLPSSTRGSYLGVIEK--IPHLLELGVNAVELLPVFEFDELEFQRHP 320
           E +V+G T    S +P   RG+Y G+     I HL EL V AVEL+PV +F  L   R  
Sbjct: 186 EAHVKGMTQTHPS-IPPELRGTYAGLAHPVIIDHLNELNVTAVELMPVHQF--LHDSRLL 245

Query: 321 NPRDHMINTWGYSTINFFAPMSRYASAGGGPLNASQEFKQMVKALHAAGIEVILDVVYNH 380
           +    + N WGY+T  FFAP  +YAS      +A  EFK MV++LH AGIEVILDVVYNH
Sbjct: 246 DL--GLRNYWGYNTFGFFAPHHQYASTRQAG-SAVAEFKTMVRSLHEAGIEVILDVVYNH 305

Query: 381 TNEGDDVNPYTTSFRGIDNKVYYMLDLKNNGQYFNFSGC-----ATHPLVLQVYSGSLMI 440
           T EG+ + P T +FRGIDN  YY L   +   Y +F+G      A HP  LQ+   S   
Sbjct: 306 TAEGNHLGP-TINFRGIDNTAYYRLMDHDLRFYKDFTGTGNSLNARHPHTLQLIMDS--- 365

Query: 441 SCNSTLIYYSTILNFRVVEYHVDGFRFDLASVLCRGTDGAPTYIRQVDMARDLNLLLFQA 500
                       L + V+E HVDGFRFDLAS L R        +  VD         F  
Sbjct: 366 ------------LRYWVIEMHVDGFRFDLASTLAR-------ELHDVDRLS----AFFDL 425

Query: 501 ISKDAILSRCKIIAEPWDCG------GLI--LWAEWNGIYRDDIRKFIKGDCGMKGSFAT 560
           + +D ++S+ K+IAEPWD G      G    LW EWNG YRD +R + +G+    G FA+
Sbjct: 426 VQQDPVVSQVKLIAEPWDVGEGGYQVGNFPGLWTEWNGKYRDTVRDYWRGEPATLGEFAS 485

Query: 561 RVAGSSDLYNVNKRKPYHGINFVIAHDGFTLRDLVSYNVKHNDANGEGGNDGCNDNFSWN 620
           R+ GSSDLY    R+P   INFV AHDGFTL DLVSYN KHN+ANGE   DG + N SWN
Sbjct: 486 RLTGSSDLYEATGRRPSASINFVTAHDGFTLNDLVSYNDKHNEANGENNRDGESYNRSWN 545

Query: 621 CGFEGETEDTSIKALRSRQMKNFHLALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDNALN 680
           CG EG T+D  I ALR+RQM+N    LM SQGTPM+  GDE G T+YGNNN Y  D+ L+
Sbjct: 546 CGVEGPTDDPDILALRARQMRNMWATLMVSQGTPMIAHGDEIGRTQYGNNNVYCQDSELS 605

Query: 681 HFLWEQLEARKRDHFRFFSEVIKFRRKHPHLFKDSF-----------QNDITW------- 740
              W  ++ +  D   F  +    R+ H    +  F             DI W       
Sbjct: 606 WMDWSLVD-KNADLLAFARKATTLRKNHKVFRRRRFFEGEPIRSGDEVRDIAWLTPSGRE 665

Query: 741 --HES---NWDNPESKFL---AYTLHDDNGEDV-----YLAFNAHDYFVNVSLPSPPTNR 745
             HE     +D   + FL   A T  D  GE V      L FNAHD+ V   +P     +
Sbjct: 666 MTHEDWGRGFDRCVAVFLNGEAITAPDARGERVVDDSFLLCFNAHDHDVEFVMPHDGYAQ 691

BLAST of IVF0022879 vs. ExPASy TrEMBL
Match: A0A1S3CS22 (isoamylase 3, chloroplastic isoform X1 OS=Cucumis melo OX=3656 GN=LOC103503684 PE=4 SV=1)

HSP 1 Score: 1495.3 bits (3870), Expect = 0.0e+00
Identity = 728/794 (91.69%), Postives = 737/794 (92.82%), Query Frame = 0

Query: 1   MIRSLPLHHSTTLQFPLFPSSCTGATHFLHFRLKRQVKLVSGSSSSDSREIFSETKQGGV 60
           MIRSLPLHHSTTLQFPLFPSSCTGATHFLHFRLKRQVKLVSGSSSSDSREIFSETKQGGV
Sbjct: 1   MIRSLPLHHSTTLQFPLFPSSCTGATHFLHFRLKRQVKLVSGSSSSDSREIFSETKQGGV 60

Query: 61  KLWTVSAFSRRDQDRVLEQEAPENLDTGPMLKIFPGQAFPLGVSEVDNGINFAIFSQHAT 120
           KLWTVSAFSRRDQDRVLEQEAPENLDTGPMLKIFPGQAFPLGVSEVDNGINFAIFSQHAT
Sbjct: 61  KLWTVSAFSRRDQDRVLEQEAPENLDTGPMLKIFPGQAFPLGVSEVDNGINFAIFSQHAT 120

Query: 121 SVTICLSLDGRIDDGMLEFKLDPDDNRTGDIWHICIQDLQRKNVLYGYRIDGPQGWHHGH 180
           SVTICLSLDGRIDDGMLEFKLDPDDNRTGDIWHICIQDLQRKNVLYGYRIDGPQGWHHGH
Sbjct: 121 SVTICLSLDGRIDDGMLEFKLDPDDNRTGDIWHICIQDLQRKNVLYGYRIDGPQGWHHGH 180

Query: 181 RYDDGTMLLDPYAKFVEGRRFFGGENKSSGFLGTYDFESLPFDWGNDYKLPNIPEKDLVI 240
           RYDDGTMLLDPYAKFVEGRRFFGGENKSSGFLGTYDFESLPFDWGNDYKLPNIPEKDLVI
Sbjct: 181 RYDDGTMLLDPYAKFVEGRRFFGGENKSSGFLGTYDFESLPFDWGNDYKLPNIPEKDLVI 240

Query: 241 YEMNVRGFTADESSGLPSSTRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHPN 300
           YEMNVRGFTADESSGLPSSTRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHPN
Sbjct: 241 YEMNVRGFTADESSGLPSSTRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHPN 300

Query: 301 PRDHMINTWGYSTINFFAPMSRYASAGGGPLNASQEFKQMVKALHAAGIEVILDVVYNHT 360
           PRDHMINTWGYSTINFFAPMSRYASAGGGPLNASQEFKQMVKALHAAGIEVILDVVYNHT
Sbjct: 301 PRDHMINTWGYSTINFFAPMSRYASAGGGPLNASQEFKQMVKALHAAGIEVILDVVYNHT 360

Query: 361 NEGDDVNPYTTSFRGIDNKVYYMLDLKNNGQYFNFSGC-----ATHPLVLQVYSGSLMIS 420
           NEGDDVNPYTTSFRGIDNKVYYMLDLKNNGQYFNFSGC       HP+V+++   S    
Sbjct: 361 NEGDDVNPYTTSFRGIDNKVYYMLDLKNNGQYFNFSGCGNTLNCNHPVVMELILES---- 420

Query: 421 CNSTLIYYSTILNFRVVEYHVDGFRFDLASVLCRGTDGAPTYIRQVDMARDLNLLLFQAI 480
                      L   VVEYHVDGFRFDLASVLCRGTDGAP               L +AI
Sbjct: 421 -----------LRHWVVEYHVDGFRFDLASVLCRGTDGAPLSAPP----------LIRAI 480

Query: 481 SKDAILSRCKIIAEPWDCGGLIL---------WAEWNGIYRDDIRKFIKGDCGMKGSFAT 540
           SKDAILSRCKIIAEPWDCGGL L         WAEWNGIYRDDIRKFIKGDCGMKGSFAT
Sbjct: 481 SKDAILSRCKIIAEPWDCGGLYLVGRFPNWDRWAEWNGIYRDDIRKFIKGDCGMKGSFAT 540

Query: 541 RVAGSSDLYNVNKRKPYHGINFVIAHDGFTLRDLVSYNVKHNDANGEGGNDGCNDNFSWN 600
           RVAGSSDLYNVNKRKPYHGINFVIAHDGFTLRDLVSYNVKHNDANGEGGNDGCNDNFSWN
Sbjct: 541 RVAGSSDLYNVNKRKPYHGINFVIAHDGFTLRDLVSYNVKHNDANGEGGNDGCNDNFSWN 600

Query: 601 CGFEGETEDTSIKALRSRQMKNFHLALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDNALN 660
           CGFEGETEDTSIKALRSRQMKNFHLALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDNALN
Sbjct: 601 CGFEGETEDTSIKALRSRQMKNFHLALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDNALN 660

Query: 661 HFLWEQLEARKRDHFRFFSEVIKFRRKHPHLF-KDSF--QNDITWHESNWDNPESKFLAY 720
           HFLWEQLEARKRDHFRFFSEVIKFRRKHPHLF +++F  +NDITWHESNWDNPESKFLAY
Sbjct: 661 HFLWEQLEARKRDHFRFFSEVIKFRRKHPHLFSRENFLNKNDITWHESNWDNPESKFLAY 720

Query: 721 TLHDDNGEDVYLAFNAHDYFVNVSLPSPPTNRRWFRVVDTNLESPHDFVLDGIPGVGSSY 778
           TLHDDNGEDVYLAFNAHDYFVNVSLPSPPTNRRWFRVVDTNLESPHDFVLDGIPGVGSSY
Sbjct: 721 TLHDDNGEDVYLAFNAHDYFVNVSLPSPPTNRRWFRVVDTNLESPHDFVLDGIPGVGSSY 769

BLAST of IVF0022879 vs. ExPASy TrEMBL
Match: A0A5D3E5B0 (Isoamylase 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G005950 PE=4 SV=1)

HSP 1 Score: 1495.3 bits (3870), Expect = 0.0e+00
Identity = 728/794 (91.69%), Postives = 737/794 (92.82%), Query Frame = 0

Query: 1   MIRSLPLHHSTTLQFPLFPSSCTGATHFLHFRLKRQVKLVSGSSSSDSREIFSETKQGGV 60
           MIRSLPLHHSTTLQFPLFPSSCTGATHFLHFRLKRQVKLVSGSSSSDSREIFSETKQGGV
Sbjct: 1   MIRSLPLHHSTTLQFPLFPSSCTGATHFLHFRLKRQVKLVSGSSSSDSREIFSETKQGGV 60

Query: 61  KLWTVSAFSRRDQDRVLEQEAPENLDTGPMLKIFPGQAFPLGVSEVDNGINFAIFSQHAT 120
           KLWTVSAFSRRDQDRVLEQEAPENLDTGPMLKIFPGQAFPLGVSEVDNGINFAIFSQHAT
Sbjct: 61  KLWTVSAFSRRDQDRVLEQEAPENLDTGPMLKIFPGQAFPLGVSEVDNGINFAIFSQHAT 120

Query: 121 SVTICLSLDGRIDDGMLEFKLDPDDNRTGDIWHICIQDLQRKNVLYGYRIDGPQGWHHGH 180
           SVTICLSLDGRIDDGMLEFKLDPDDNRTGDIWHICIQDLQRKNVLYGYRIDGPQGWHHGH
Sbjct: 121 SVTICLSLDGRIDDGMLEFKLDPDDNRTGDIWHICIQDLQRKNVLYGYRIDGPQGWHHGH 180

Query: 181 RYDDGTMLLDPYAKFVEGRRFFGGENKSSGFLGTYDFESLPFDWGNDYKLPNIPEKDLVI 240
           RYDDGTMLLDPYAKFVEGRRFFGGENKSSGFLGTYDFESLPFDWGNDYKLPNIPEKDLVI
Sbjct: 181 RYDDGTMLLDPYAKFVEGRRFFGGENKSSGFLGTYDFESLPFDWGNDYKLPNIPEKDLVI 240

Query: 241 YEMNVRGFTADESSGLPSSTRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHPN 300
           YEMNVRGFTADESSGLPSSTRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHPN
Sbjct: 241 YEMNVRGFTADESSGLPSSTRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHPN 300

Query: 301 PRDHMINTWGYSTINFFAPMSRYASAGGGPLNASQEFKQMVKALHAAGIEVILDVVYNHT 360
           PRDHMINTWGYSTINFFAPMSRYASAGGGPLNASQEFKQMVKALHAAGIEVILDVVYNHT
Sbjct: 301 PRDHMINTWGYSTINFFAPMSRYASAGGGPLNASQEFKQMVKALHAAGIEVILDVVYNHT 360

Query: 361 NEGDDVNPYTTSFRGIDNKVYYMLDLKNNGQYFNFSGC-----ATHPLVLQVYSGSLMIS 420
           NEGDDVNPYTTSFRGIDNKVYYMLDLKNNGQYFNFSGC       HP+V+++   S    
Sbjct: 361 NEGDDVNPYTTSFRGIDNKVYYMLDLKNNGQYFNFSGCGNTLNCNHPVVMELILES---- 420

Query: 421 CNSTLIYYSTILNFRVVEYHVDGFRFDLASVLCRGTDGAPTYIRQVDMARDLNLLLFQAI 480
                      L   VVEYHVDGFRFDLASVLCRGTDGAP               L +AI
Sbjct: 421 -----------LRHWVVEYHVDGFRFDLASVLCRGTDGAPLSAPP----------LIRAI 480

Query: 481 SKDAILSRCKIIAEPWDCGGLIL---------WAEWNGIYRDDIRKFIKGDCGMKGSFAT 540
           SKDAILSRCKIIAEPWDCGGL L         WAEWNGIYRDDIRKFIKGDCGMKGSFAT
Sbjct: 481 SKDAILSRCKIIAEPWDCGGLYLVGRFPNWDRWAEWNGIYRDDIRKFIKGDCGMKGSFAT 540

Query: 541 RVAGSSDLYNVNKRKPYHGINFVIAHDGFTLRDLVSYNVKHNDANGEGGNDGCNDNFSWN 600
           RVAGSSDLYNVNKRKPYHGINFVIAHDGFTLRDLVSYNVKHNDANGEGGNDGCNDNFSWN
Sbjct: 541 RVAGSSDLYNVNKRKPYHGINFVIAHDGFTLRDLVSYNVKHNDANGEGGNDGCNDNFSWN 600

Query: 601 CGFEGETEDTSIKALRSRQMKNFHLALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDNALN 660
           CGFEGETEDTSIKALRSRQMKNFHLALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDNALN
Sbjct: 601 CGFEGETEDTSIKALRSRQMKNFHLALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDNALN 660

Query: 661 HFLWEQLEARKRDHFRFFSEVIKFRRKHPHLF-KDSF--QNDITWHESNWDNPESKFLAY 720
           HFLWEQLEARKRDHFRFFSEVIKFRRKHPHLF +++F  +NDITWHESNWDNPESKFLAY
Sbjct: 661 HFLWEQLEARKRDHFRFFSEVIKFRRKHPHLFSRENFLNKNDITWHESNWDNPESKFLAY 720

Query: 721 TLHDDNGEDVYLAFNAHDYFVNVSLPSPPTNRRWFRVVDTNLESPHDFVLDGIPGVGSSY 778
           TLHDDNGEDVYLAFNAHDYFVNVSLPSPPTNRRWFRVVDTNLESPHDFVLDGIPGVGSSY
Sbjct: 721 TLHDDNGEDVYLAFNAHDYFVNVSLPSPPTNRRWFRVVDTNLESPHDFVLDGIPGVGSSY 769

BLAST of IVF0022879 vs. ExPASy TrEMBL
Match: A0A5A7TAT5 (Isoamylase 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold92G002290 PE=4 SV=1)

HSP 1 Score: 1490.7 bits (3858), Expect = 0.0e+00
Identity = 728/795 (91.57%), Postives = 737/795 (92.70%), Query Frame = 0

Query: 1   MIRSLPLHHSTTLQFPLFPSSCTGATHFLHFRLKRQVKLVSGSSSSDSREIFSETKQGGV 60
           MIRSLPLHHSTTLQFPLFPSSCTGATHFLHFRLKRQVKLVSGSSSSDSREIFSETKQGGV
Sbjct: 1   MIRSLPLHHSTTLQFPLFPSSCTGATHFLHFRLKRQVKLVSGSSSSDSREIFSETKQGGV 60

Query: 61  KLWTVSAFSRRDQDRVLEQEAPENLDTGPMLKIFPGQAFPLGVSEVDNGINFAIFSQHAT 120
           KLWTVSAFSRRDQDRVLEQEAPENLDTGPMLKIFPGQAFPLGVSEVDNGINFAIFSQHAT
Sbjct: 61  KLWTVSAFSRRDQDRVLEQEAPENLDTGPMLKIFPGQAFPLGVSEVDNGINFAIFSQHAT 120

Query: 121 SVTICLSLDGRIDDGMLEFKLDPDDNRTGDIWHICI-QDLQRKNVLYGYRIDGPQGWHHG 180
           SVTICLSLDGRIDDGMLEFKLDPDDNRTGDIWHICI QDLQRKNVLYGYRIDGPQGWHHG
Sbjct: 121 SVTICLSLDGRIDDGMLEFKLDPDDNRTGDIWHICIQQDLQRKNVLYGYRIDGPQGWHHG 180

Query: 181 HRYDDGTMLLDPYAKFVEGRRFFGGENKSSGFLGTYDFESLPFDWGNDYKLPNIPEKDLV 240
           HRYDDGTMLLDPYAKFVEGRRFFGGENKSSGFLGTYDFESLPFDWGNDYKLPNIPEKDLV
Sbjct: 181 HRYDDGTMLLDPYAKFVEGRRFFGGENKSSGFLGTYDFESLPFDWGNDYKLPNIPEKDLV 240

Query: 241 IYEMNVRGFTADESSGLPSSTRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHP 300
           IYEMNVRGFTADESSGLPSSTRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHP
Sbjct: 241 IYEMNVRGFTADESSGLPSSTRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHP 300

Query: 301 NPRDHMINTWGYSTINFFAPMSRYASAGGGPLNASQEFKQMVKALHAAGIEVILDVVYNH 360
           NPRDHMINTWGYSTINFFAPMSRYASAGGGPLNASQEFKQMVKALHAAGIEVILDVVYNH
Sbjct: 301 NPRDHMINTWGYSTINFFAPMSRYASAGGGPLNASQEFKQMVKALHAAGIEVILDVVYNH 360

Query: 361 TNEGDDVNPYTTSFRGIDNKVYYMLDLKNNGQYFNFSGC-----ATHPLVLQVYSGSLMI 420
           TNEGDDVNPYTTSFRGIDNKVYYMLDLKNNGQYFNFSGC       HP+V+++   S   
Sbjct: 361 TNEGDDVNPYTTSFRGIDNKVYYMLDLKNNGQYFNFSGCGNTLNCNHPVVMELILES--- 420

Query: 421 SCNSTLIYYSTILNFRVVEYHVDGFRFDLASVLCRGTDGAPTYIRQVDMARDLNLLLFQA 480
                       L   VVEYHVDGFRFDLASVLCRGTDGAP               L +A
Sbjct: 421 ------------LRHWVVEYHVDGFRFDLASVLCRGTDGAPLSAPP----------LIRA 480

Query: 481 ISKDAILSRCKIIAEPWDCGGLIL---------WAEWNGIYRDDIRKFIKGDCGMKGSFA 540
           ISKDAILSRCKIIAEPWDCGGL L         WAEWNGIYRDDIRKFIKGDCGMKGSFA
Sbjct: 481 ISKDAILSRCKIIAEPWDCGGLYLVGRFPNWDRWAEWNGIYRDDIRKFIKGDCGMKGSFA 540

Query: 541 TRVAGSSDLYNVNKRKPYHGINFVIAHDGFTLRDLVSYNVKHNDANGEGGNDGCNDNFSW 600
           TRVAGSSDLYNVNKRKPYHGINFVIAHDGFTLRDLVSYNVKHNDANGEGGNDGCNDNFSW
Sbjct: 541 TRVAGSSDLYNVNKRKPYHGINFVIAHDGFTLRDLVSYNVKHNDANGEGGNDGCNDNFSW 600

Query: 601 NCGFEGETEDTSIKALRSRQMKNFHLALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDNAL 660
           NCGFEGETEDTSIKALRSRQMKNFHLALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDNAL
Sbjct: 601 NCGFEGETEDTSIKALRSRQMKNFHLALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDNAL 660

Query: 661 NHFLWEQLEARKRDHFRFFSEVIKFRRKHPHLF-KDSF--QNDITWHESNWDNPESKFLA 720
           NHFLWEQLEARKRDHFRFFSEVIKFRRKHPHLF +++F  +NDITWHESNWDNPESKFLA
Sbjct: 661 NHFLWEQLEARKRDHFRFFSEVIKFRRKHPHLFSRENFLNKNDITWHESNWDNPESKFLA 720

Query: 721 YTLHDDNGEDVYLAFNAHDYFVNVSLPSPPTNRRWFRVVDTNLESPHDFVLDGIPGVGSS 778
           YTLHDDNGEDVYLAFNAHDYFVNVSLPSPPTNRRWFRVVDTNLESPHDFVLDGIPGVGSS
Sbjct: 721 YTLHDDNGEDVYLAFNAHDYFVNVSLPSPPTNRRWFRVVDTNLESPHDFVLDGIPGVGSS 770

BLAST of IVF0022879 vs. ExPASy TrEMBL
Match: A0A0A0LE02 (Aamy domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G889910 PE=4 SV=1)

HSP 1 Score: 1442.6 bits (3733), Expect = 0.0e+00
Identity = 704/795 (88.55%), Postives = 723/795 (90.94%), Query Frame = 0

Query: 1   MIRSLPLHH-STTLQFPLFPSSCTGATHFLHFRLKRQVKLVSGSSSSDSREIFSETKQGG 60
           MIRSLPLHH STTL FPL PSSCTGATH LHFRLKRQVKLV GSSSSDSREIFSETKQGG
Sbjct: 1   MIRSLPLHHYSTTLHFPLLPSSCTGATHSLHFRLKRQVKLVPGSSSSDSREIFSETKQGG 60

Query: 61  VKLWTVSAFSRRDQDRVLEQEAPENLDTGPMLKIFPGQAFPLGVSEVDNGINFAIFSQHA 120
           VKLWTVSA S RDQDRVLEQEAPENLDTGPMLKIFPGQAFPLGVSEVDNGINFAIFSQHA
Sbjct: 61  VKLWTVSALSSRDQDRVLEQEAPENLDTGPMLKIFPGQAFPLGVSEVDNGINFAIFSQHA 120

Query: 121 TSVTICLSLDGRIDDGMLEFKLDPDDNRTGDIWHICIQDLQRKNVLYGYRIDGPQGWHHG 180
           TSVT+CLSLDGRIDDGMLEFKLDPDDNRTGDIWHICIQDL+RKNVLYGYRIDGPQGWHHG
Sbjct: 121 TSVTLCLSLDGRIDDGMLEFKLDPDDNRTGDIWHICIQDLRRKNVLYGYRIDGPQGWHHG 180

Query: 181 HRYDDGTMLLDPYAKFVEGRRFFGGENKSSGFLGTYDFESLPFDWGNDYKLPNIPEKDLV 240
           HRYD GT+LLDPYAKFVEGRR+FGGENKS+GFLGTYDF+SLPFDWGNDYKLPNIPEKDLV
Sbjct: 181 HRYDAGTVLLDPYAKFVEGRRYFGGENKSTGFLGTYDFDSLPFDWGNDYKLPNIPEKDLV 240

Query: 241 IYEMNVRGFTADESSGLPSSTRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHP 300
           IYEMNVRGFTADESSGLPSSTRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHP
Sbjct: 241 IYEMNVRGFTADESSGLPSSTRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHP 300

Query: 301 NPRDHMINTWGYSTINFFAPMSRYASAGGGPLNASQEFKQMVKALHAAGIEVILDVVYNH 360
           NPRDHMINTWGYSTINFFAPMSRYASAGGGPLNAS EFKQMVKALHAAGIEVILDVVYNH
Sbjct: 301 NPRDHMINTWGYSTINFFAPMSRYASAGGGPLNASLEFKQMVKALHAAGIEVILDVVYNH 360

Query: 361 TNEGDDVNPYTTSFRGIDNKVYYMLDLKNNGQYFNFSGC-----ATHPLVLQVYSGSLMI 420
           TNE DDV PY TSFRGIDNKVYYMLDLKNNGQYFNFSGC       HP+V+++   S   
Sbjct: 361 TNEADDVIPYITSFRGIDNKVYYMLDLKNNGQYFNFSGCGNTLNCNHPVVMELILES--- 420

Query: 421 SCNSTLIYYSTILNFRVVEYHVDGFRFDLASVLCRGTDGAPTYIRQVDMARDLNLLLFQA 480
                       L   VVEYHVDGFRFDLASVLCRGTDGAP               L +A
Sbjct: 421 ------------LRHWVVEYHVDGFRFDLASVLCRGTDGAPLSAPP----------LIRA 480

Query: 481 ISKDAILSRCKIIAEPWDCGGLIL---------WAEWNGIYRDDIRKFIKGDCGMKGSFA 540
           ISKDAILSRCKIIAEPWDCGGL L         WAEWNGIYRDDIRKFIKGDCGMKGSFA
Sbjct: 481 ISKDAILSRCKIIAEPWDCGGLYLVGRFPNWDRWAEWNGIYRDDIRKFIKGDCGMKGSFA 540

Query: 541 TRVAGSSDLYNVNKRKPYHGINFVIAHDGFTLRDLVSYNVKHNDANGEGGNDGCNDNFSW 600
           TRVAGSSDLY VNKRKP HGINFVIAHDGFTLRDLVSYNVKHNDANGEGGNDGCNDNFSW
Sbjct: 541 TRVAGSSDLYKVNKRKPCHGINFVIAHDGFTLRDLVSYNVKHNDANGEGGNDGCNDNFSW 600

Query: 601 NCGFEGETEDTSIKALRSRQMKNFHLALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDNAL 660
           NCGFEGETEDTSIKALR+RQMKNFHLALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDNAL
Sbjct: 601 NCGFEGETEDTSIKALRTRQMKNFHLALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDNAL 660

Query: 661 NHFLWEQLEARKRDHFRFFSEVIKFRRKHPHLF-KDSF--QNDITWHESNWDNPESKFLA 720
           NHFLWEQLEARKRDHFRFFSEVIKFRRKHPHLF +++F  +NDITWHESNWDNPESKFLA
Sbjct: 661 NHFLWEQLEARKRDHFRFFSEVIKFRRKHPHLFSRENFLNKNDITWHESNWDNPESKFLA 720

Query: 721 YTLHDDNGEDVYLAFNAHDYFVNVSLPSPPTNRRWFRVVDTNLESPHDFVLDGIPGVGSS 778
           YTLHDDNGEDVYLAFNAH+YFVNVSLPSPPT R+WFRVVDTNLESPHDFVLDGIPGVGSS
Sbjct: 721 YTLHDDNGEDVYLAFNAHEYFVNVSLPSPPTKRKWFRVVDTNLESPHDFVLDGIPGVGSS 770

BLAST of IVF0022879 vs. ExPASy TrEMBL
Match: A0A6J1F9D1 (isoamylase 3, chloroplastic isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111443282 PE=4 SV=1)

HSP 1 Score: 1360.1 bits (3519), Expect = 0.0e+00
Identity = 661/794 (83.25%), Postives = 696/794 (87.66%), Query Frame = 0

Query: 1   MIRSLPLHHSTTLQFPLFPSSCTGATHFLHFRLKRQVKLVSGSSSSDSREIFSETKQGGV 60
           MIRS+PLHHS  LQ PLF SSCTGA H + FRLK Q K   GSSSS S+E+FSETKQGGV
Sbjct: 1   MIRSIPLHHSAPLQLPLFASSCTGAAHSVQFRLKPQGKRAPGSSSSSSQEVFSETKQGGV 60

Query: 61  KLWTVSAFSRRDQDRVLEQEAPENLDTGPMLKIFPGQAFPLGVSEVDNGINFAIFSQHAT 120
           K+ TVSAF RRDQDRVLEQEAPENLDTGPMLK+ PGQAFPLGVSEVDNGINFAIFSQHAT
Sbjct: 61  KICTVSAFGRRDQDRVLEQEAPENLDTGPMLKVVPGQAFPLGVSEVDNGINFAIFSQHAT 120

Query: 121 SVTICLSLDGRIDDGMLEFKLDPDDNRTGDIWHICIQDLQRKNVLYGYRIDGPQGWHHGH 180
           +VTICLS + RIDD  LEFKLDPDDNRTGDIWHICIQDL RKNVLYGYRIDGPQGWHHGH
Sbjct: 121 AVTICLSFNERIDDRTLEFKLDPDDNRTGDIWHICIQDLPRKNVLYGYRIDGPQGWHHGH 180

Query: 181 RYDDGTMLLDPYAKFVEGRRFFGGENKSSGFLGTYDFESLPFDWGNDYKLPNIPEKDLVI 240
           R+DD  +LLDPYAKFVEGRR+FGGENKSSGFLGTYDF+SLPFDWG+DYKLPNI EKDLVI
Sbjct: 181 RFDDSIVLLDPYAKFVEGRRYFGGENKSSGFLGTYDFDSLPFDWGDDYKLPNILEKDLVI 240

Query: 241 YEMNVRGFTADESSGLPSSTRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHPN 300
           YEMNVR FTADESSGL SS RGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHPN
Sbjct: 241 YEMNVRAFTADESSGLASSIRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHPN 300

Query: 301 PRDHMINTWGYSTINFFAPMSRYASAGGGPLNASQEFKQMVKALHAAGIEVILDVVYNHT 360
           PR+HMINTWGYSTINFFAPMSRYASAGGGPLNAS+EFK+MVKALH AGIEVILDVVYNHT
Sbjct: 301 PRNHMINTWGYSTINFFAPMSRYASAGGGPLNASREFKEMVKALHGAGIEVILDVVYNHT 360

Query: 361 NEGDDVNPYTTSFRGIDNKVYYMLDLKNNGQYFNFSGC-----ATHPLVLQVYSGSLMIS 420
           NE DDV PYTTSFRGIDNKVYYMLDL+NNGQ+ NFSGC       HP+V+++   S    
Sbjct: 361 NEADDVYPYTTSFRGIDNKVYYMLDLRNNGQFLNFSGCGNTLNCNHPVVMELILDS---- 420

Query: 421 CNSTLIYYSTILNFRVVEYHVDGFRFDLASVLCRGTDGAPTYIRQVDMARDLNLLLFQAI 480
                      L   VVEYHVDGFRFDLASVLCRG DGAP               L +AI
Sbjct: 421 -----------LRHWVVEYHVDGFRFDLASVLCRGPDGAPLSAPP----------LIRAI 480

Query: 481 SKDAILSRCKIIAEPWDCGGLIL---------WAEWNGIYRDDIRKFIKGDCGMKGSFAT 540
           SKDAILSRCKIIAEPWDCGGL L         WAEWNGIYRDDIRKFIKGDCGMKGSFAT
Sbjct: 481 SKDAILSRCKIIAEPWDCGGLYLVGRFPNWDRWAEWNGIYRDDIRKFIKGDCGMKGSFAT 540

Query: 541 RVAGSSDLYNVNKRKPYHGINFVIAHDGFTLRDLVSYNVKHNDANGEGGNDGCNDNFSWN 600
           RV+GSSDLY VN RKPYHGINFVIAHDGFTLRDLVSYNVKHN+ANGEGGNDGCNDNFSWN
Sbjct: 541 RVSGSSDLYKVNNRKPYHGINFVIAHDGFTLRDLVSYNVKHNNANGEGGNDGCNDNFSWN 600

Query: 601 CGFEGETEDTSIKALRSRQMKNFHLALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDNALN 660
           CGFEGETED +I+ALRSRQMKNFHLALMTSQGTPMMLMGDEYGHTRYGNNNSYGHD ALN
Sbjct: 601 CGFEGETEDANIRALRSRQMKNFHLALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDTALN 660

Query: 661 HFLWEQLEARKRDHFRFFSEVIKFRRKHPHLF-KDSF--QNDITWHESNWDNPESKFLAY 720
           HFLW QLEARKRDHFRFFSEVIKFRRKHPHLF +++F  +NDITWHESNWDNPESKFLAY
Sbjct: 661 HFLWGQLEARKRDHFRFFSEVIKFRRKHPHLFSRENFLGENDITWHESNWDNPESKFLAY 720

Query: 721 TLHDDNGEDVYLAFNAHDYFVNVSLPSPPTNRRWFRVVDTNLESPHDFVLDGIPGVGSSY 778
           TLHD NGEDVYLAFNAHDYFV+VSLPSPPTNR+WFRVVDTNLESP DFVLDGI G+GS+Y
Sbjct: 721 TLHDGNGEDVYLAFNAHDYFVSVSLPSPPTNRKWFRVVDTNLESPQDFVLDGIRGIGSTY 769

BLAST of IVF0022879 vs. NCBI nr
Match: XP_008466194.1 (PREDICTED: isoamylase 3, chloroplastic isoform X1 [Cucumis melo] >XP_008466195.1 PREDICTED: isoamylase 3, chloroplastic isoform X1 [Cucumis melo] >TYK31297.1 isoamylase 3 [Cucumis melo var. makuwa])

HSP 1 Score: 1489 bits (3854), Expect = 0.0
Identity = 728/794 (91.69%), Postives = 737/794 (92.82%), Query Frame = 0

Query: 1   MIRSLPLHHSTTLQFPLFPSSCTGATHFLHFRLKRQVKLVSGSSSSDSREIFSETKQGGV 60
           MIRSLPLHHSTTLQFPLFPSSCTGATHFLHFRLKRQVKLVSGSSSSDSREIFSETKQGGV
Sbjct: 1   MIRSLPLHHSTTLQFPLFPSSCTGATHFLHFRLKRQVKLVSGSSSSDSREIFSETKQGGV 60

Query: 61  KLWTVSAFSRRDQDRVLEQEAPENLDTGPMLKIFPGQAFPLGVSEVDNGINFAIFSQHAT 120
           KLWTVSAFSRRDQDRVLEQEAPENLDTGPMLKIFPGQAFPLGVSEVDNGINFAIFSQHAT
Sbjct: 61  KLWTVSAFSRRDQDRVLEQEAPENLDTGPMLKIFPGQAFPLGVSEVDNGINFAIFSQHAT 120

Query: 121 SVTICLSLDGRIDDGMLEFKLDPDDNRTGDIWHICIQDLQRKNVLYGYRIDGPQGWHHGH 180
           SVTICLSLDGRIDDGMLEFKLDPDDNRTGDIWHICIQDLQRKNVLYGYRIDGPQGWHHGH
Sbjct: 121 SVTICLSLDGRIDDGMLEFKLDPDDNRTGDIWHICIQDLQRKNVLYGYRIDGPQGWHHGH 180

Query: 181 RYDDGTMLLDPYAKFVEGRRFFGGENKSSGFLGTYDFESLPFDWGNDYKLPNIPEKDLVI 240
           RYDDGTMLLDPYAKFVEGRRFFGGENKSSGFLGTYDFESLPFDWGNDYKLPNIPEKDLVI
Sbjct: 181 RYDDGTMLLDPYAKFVEGRRFFGGENKSSGFLGTYDFESLPFDWGNDYKLPNIPEKDLVI 240

Query: 241 YEMNVRGFTADESSGLPSSTRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHPN 300
           YEMNVRGFTADESSGLPSSTRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHPN
Sbjct: 241 YEMNVRGFTADESSGLPSSTRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHPN 300

Query: 301 PRDHMINTWGYSTINFFAPMSRYASAGGGPLNASQEFKQMVKALHAAGIEVILDVVYNHT 360
           PRDHMINTWGYSTINFFAPMSRYASAGGGPLNASQEFKQMVKALHAAGIEVILDVVYNHT
Sbjct: 301 PRDHMINTWGYSTINFFAPMSRYASAGGGPLNASQEFKQMVKALHAAGIEVILDVVYNHT 360

Query: 361 NEGDDVNPYTTSFRGIDNKVYYMLDLKNNGQYFNFSGCAT-----HPLVLQVYSGSLMIS 420
           NEGDDVNPYTTSFRGIDNKVYYMLDLKNNGQYFNFSGC       HP+V+++   SL   
Sbjct: 361 NEGDDVNPYTTSFRGIDNKVYYMLDLKNNGQYFNFSGCGNTLNCNHPVVMELILESL--- 420

Query: 421 CNSTLIYYSTILNFRVVEYHVDGFRFDLASVLCRGTDGAPTYIRQVDMARDLNLLLFQAI 480
                          VVEYHVDGFRFDLASVLCRGTDGAP               L +AI
Sbjct: 421 ------------RHWVVEYHVDGFRFDLASVLCRGTDGAPLSAPP----------LIRAI 480

Query: 481 SKDAILSRCKIIAEPWDCGGLIL---------WAEWNGIYRDDIRKFIKGDCGMKGSFAT 540
           SKDAILSRCKIIAEPWDCGGL L         WAEWNGIYRDDIRKFIKGDCGMKGSFAT
Sbjct: 481 SKDAILSRCKIIAEPWDCGGLYLVGRFPNWDRWAEWNGIYRDDIRKFIKGDCGMKGSFAT 540

Query: 541 RVAGSSDLYNVNKRKPYHGINFVIAHDGFTLRDLVSYNVKHNDANGEGGNDGCNDNFSWN 600
           RVAGSSDLYNVNKRKPYHGINFVIAHDGFTLRDLVSYNVKHNDANGEGGNDGCNDNFSWN
Sbjct: 541 RVAGSSDLYNVNKRKPYHGINFVIAHDGFTLRDLVSYNVKHNDANGEGGNDGCNDNFSWN 600

Query: 601 CGFEGETEDTSIKALRSRQMKNFHLALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDNALN 660
           CGFEGETEDTSIKALRSRQMKNFHLALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDNALN
Sbjct: 601 CGFEGETEDTSIKALRSRQMKNFHLALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDNALN 660

Query: 661 HFLWEQLEARKRDHFRFFSEVIKFRRKHPHLF-KDSF--QNDITWHESNWDNPESKFLAY 720
           HFLWEQLEARKRDHFRFFSEVIKFRRKHPHLF +++F  +NDITWHESNWDNPESKFLAY
Sbjct: 661 HFLWEQLEARKRDHFRFFSEVIKFRRKHPHLFSRENFLNKNDITWHESNWDNPESKFLAY 720

Query: 721 TLHDDNGEDVYLAFNAHDYFVNVSLPSPPTNRRWFRVVDTNLESPHDFVLDGIPGVGSSY 777
           TLHDDNGEDVYLAFNAHDYFVNVSLPSPPTNRRWFRVVDTNLESPHDFVLDGIPGVGSSY
Sbjct: 721 TLHDDNGEDVYLAFNAHDYFVNVSLPSPPTNRRWFRVVDTNLESPHDFVLDGIPGVGSSY 769

BLAST of IVF0022879 vs. NCBI nr
Match: KAA0038687.1 (isoamylase 3 [Cucumis melo var. makuwa])

HSP 1 Score: 1484 bits (3842), Expect = 0.0
Identity = 728/795 (91.57%), Postives = 737/795 (92.70%), Query Frame = 0

Query: 1   MIRSLPLHHSTTLQFPLFPSSCTGATHFLHFRLKRQVKLVSGSSSSDSREIFSETKQGGV 60
           MIRSLPLHHSTTLQFPLFPSSCTGATHFLHFRLKRQVKLVSGSSSSDSREIFSETKQGGV
Sbjct: 1   MIRSLPLHHSTTLQFPLFPSSCTGATHFLHFRLKRQVKLVSGSSSSDSREIFSETKQGGV 60

Query: 61  KLWTVSAFSRRDQDRVLEQEAPENLDTGPMLKIFPGQAFPLGVSEVDNGINFAIFSQHAT 120
           KLWTVSAFSRRDQDRVLEQEAPENLDTGPMLKIFPGQAFPLGVSEVDNGINFAIFSQHAT
Sbjct: 61  KLWTVSAFSRRDQDRVLEQEAPENLDTGPMLKIFPGQAFPLGVSEVDNGINFAIFSQHAT 120

Query: 121 SVTICLSLDGRIDDGMLEFKLDPDDNRTGDIWHICIQ-DLQRKNVLYGYRIDGPQGWHHG 180
           SVTICLSLDGRIDDGMLEFKLDPDDNRTGDIWHICIQ DLQRKNVLYGYRIDGPQGWHHG
Sbjct: 121 SVTICLSLDGRIDDGMLEFKLDPDDNRTGDIWHICIQQDLQRKNVLYGYRIDGPQGWHHG 180

Query: 181 HRYDDGTMLLDPYAKFVEGRRFFGGENKSSGFLGTYDFESLPFDWGNDYKLPNIPEKDLV 240
           HRYDDGTMLLDPYAKFVEGRRFFGGENKSSGFLGTYDFESLPFDWGNDYKLPNIPEKDLV
Sbjct: 181 HRYDDGTMLLDPYAKFVEGRRFFGGENKSSGFLGTYDFESLPFDWGNDYKLPNIPEKDLV 240

Query: 241 IYEMNVRGFTADESSGLPSSTRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHP 300
           IYEMNVRGFTADESSGLPSSTRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHP
Sbjct: 241 IYEMNVRGFTADESSGLPSSTRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHP 300

Query: 301 NPRDHMINTWGYSTINFFAPMSRYASAGGGPLNASQEFKQMVKALHAAGIEVILDVVYNH 360
           NPRDHMINTWGYSTINFFAPMSRYASAGGGPLNASQEFKQMVKALHAAGIEVILDVVYNH
Sbjct: 301 NPRDHMINTWGYSTINFFAPMSRYASAGGGPLNASQEFKQMVKALHAAGIEVILDVVYNH 360

Query: 361 TNEGDDVNPYTTSFRGIDNKVYYMLDLKNNGQYFNFSGCAT-----HPLVLQVYSGSLMI 420
           TNEGDDVNPYTTSFRGIDNKVYYMLDLKNNGQYFNFSGC       HP+V+++   SL  
Sbjct: 361 TNEGDDVNPYTTSFRGIDNKVYYMLDLKNNGQYFNFSGCGNTLNCNHPVVMELILESL-- 420

Query: 421 SCNSTLIYYSTILNFRVVEYHVDGFRFDLASVLCRGTDGAPTYIRQVDMARDLNLLLFQA 480
                           VVEYHVDGFRFDLASVLCRGTDGAP               L +A
Sbjct: 421 -------------RHWVVEYHVDGFRFDLASVLCRGTDGAPLSAPP----------LIRA 480

Query: 481 ISKDAILSRCKIIAEPWDCGGLIL---------WAEWNGIYRDDIRKFIKGDCGMKGSFA 540
           ISKDAILSRCKIIAEPWDCGGL L         WAEWNGIYRDDIRKFIKGDCGMKGSFA
Sbjct: 481 ISKDAILSRCKIIAEPWDCGGLYLVGRFPNWDRWAEWNGIYRDDIRKFIKGDCGMKGSFA 540

Query: 541 TRVAGSSDLYNVNKRKPYHGINFVIAHDGFTLRDLVSYNVKHNDANGEGGNDGCNDNFSW 600
           TRVAGSSDLYNVNKRKPYHGINFVIAHDGFTLRDLVSYNVKHNDANGEGGNDGCNDNFSW
Sbjct: 541 TRVAGSSDLYNVNKRKPYHGINFVIAHDGFTLRDLVSYNVKHNDANGEGGNDGCNDNFSW 600

Query: 601 NCGFEGETEDTSIKALRSRQMKNFHLALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDNAL 660
           NCGFEGETEDTSIKALRSRQMKNFHLALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDNAL
Sbjct: 601 NCGFEGETEDTSIKALRSRQMKNFHLALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDNAL 660

Query: 661 NHFLWEQLEARKRDHFRFFSEVIKFRRKHPHLF-KDSF--QNDITWHESNWDNPESKFLA 720
           NHFLWEQLEARKRDHFRFFSEVIKFRRKHPHLF +++F  +NDITWHESNWDNPESKFLA
Sbjct: 661 NHFLWEQLEARKRDHFRFFSEVIKFRRKHPHLFSRENFLNKNDITWHESNWDNPESKFLA 720

Query: 721 YTLHDDNGEDVYLAFNAHDYFVNVSLPSPPTNRRWFRVVDTNLESPHDFVLDGIPGVGSS 777
           YTLHDDNGEDVYLAFNAHDYFVNVSLPSPPTNRRWFRVVDTNLESPHDFVLDGIPGVGSS
Sbjct: 721 YTLHDDNGEDVYLAFNAHDYFVNVSLPSPPTNRRWFRVVDTNLESPHDFVLDGIPGVGSS 770

BLAST of IVF0022879 vs. NCBI nr
Match: XP_011652565.1 (isoamylase 3, chloroplastic isoform X1 [Cucumis sativus] >KGN60235.1 hypothetical protein Csa_002537 [Cucumis sativus])

HSP 1 Score: 1436 bits (3717), Expect = 0.0
Identity = 704/795 (88.55%), Postives = 723/795 (90.94%), Query Frame = 0

Query: 1   MIRSLPLHH-STTLQFPLFPSSCTGATHFLHFRLKRQVKLVSGSSSSDSREIFSETKQGG 60
           MIRSLPLHH STTL FPL PSSCTGATH LHFRLKRQVKLV GSSSSDSREIFSETKQGG
Sbjct: 1   MIRSLPLHHYSTTLHFPLLPSSCTGATHSLHFRLKRQVKLVPGSSSSDSREIFSETKQGG 60

Query: 61  VKLWTVSAFSRRDQDRVLEQEAPENLDTGPMLKIFPGQAFPLGVSEVDNGINFAIFSQHA 120
           VKLWTVSA S RDQDRVLEQEAPENLDTGPMLKIFPGQAFPLGVSEVDNGINFAIFSQHA
Sbjct: 61  VKLWTVSALSSRDQDRVLEQEAPENLDTGPMLKIFPGQAFPLGVSEVDNGINFAIFSQHA 120

Query: 121 TSVTICLSLDGRIDDGMLEFKLDPDDNRTGDIWHICIQDLQRKNVLYGYRIDGPQGWHHG 180
           TSVT+CLSLDGRIDDGMLEFKLDPDDNRTGDIWHICIQDL+RKNVLYGYRIDGPQGWHHG
Sbjct: 121 TSVTLCLSLDGRIDDGMLEFKLDPDDNRTGDIWHICIQDLRRKNVLYGYRIDGPQGWHHG 180

Query: 181 HRYDDGTMLLDPYAKFVEGRRFFGGENKSSGFLGTYDFESLPFDWGNDYKLPNIPEKDLV 240
           HRYD GT+LLDPYAKFVEGRR+FGGENKS+GFLGTYDF+SLPFDWGNDYKLPNIPEKDLV
Sbjct: 181 HRYDAGTVLLDPYAKFVEGRRYFGGENKSTGFLGTYDFDSLPFDWGNDYKLPNIPEKDLV 240

Query: 241 IYEMNVRGFTADESSGLPSSTRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHP 300
           IYEMNVRGFTADESSGLPSSTRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHP
Sbjct: 241 IYEMNVRGFTADESSGLPSSTRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHP 300

Query: 301 NPRDHMINTWGYSTINFFAPMSRYASAGGGPLNASQEFKQMVKALHAAGIEVILDVVYNH 360
           NPRDHMINTWGYSTINFFAPMSRYASAGGGPLNAS EFKQMVKALHAAGIEVILDVVYNH
Sbjct: 301 NPRDHMINTWGYSTINFFAPMSRYASAGGGPLNASLEFKQMVKALHAAGIEVILDVVYNH 360

Query: 361 TNEGDDVNPYTTSFRGIDNKVYYMLDLKNNGQYFNFSGCAT-----HPLVLQVYSGSLMI 420
           TNE DDV PY TSFRGIDNKVYYMLDLKNNGQYFNFSGC       HP+V+++   SL  
Sbjct: 361 TNEADDVIPYITSFRGIDNKVYYMLDLKNNGQYFNFSGCGNTLNCNHPVVMELILESL-- 420

Query: 421 SCNSTLIYYSTILNFRVVEYHVDGFRFDLASVLCRGTDGAPTYIRQVDMARDLNLLLFQA 480
                           VVEYHVDGFRFDLASVLCRGTDGAP               L +A
Sbjct: 421 -------------RHWVVEYHVDGFRFDLASVLCRGTDGAPLSAPP----------LIRA 480

Query: 481 ISKDAILSRCKIIAEPWDCGGLIL---------WAEWNGIYRDDIRKFIKGDCGMKGSFA 540
           ISKDAILSRCKIIAEPWDCGGL L         WAEWNGIYRDDIRKFIKGDCGMKGSFA
Sbjct: 481 ISKDAILSRCKIIAEPWDCGGLYLVGRFPNWDRWAEWNGIYRDDIRKFIKGDCGMKGSFA 540

Query: 541 TRVAGSSDLYNVNKRKPYHGINFVIAHDGFTLRDLVSYNVKHNDANGEGGNDGCNDNFSW 600
           TRVAGSSDLY VNKRKP HGINFVIAHDGFTLRDLVSYNVKHNDANGEGGNDGCNDNFSW
Sbjct: 541 TRVAGSSDLYKVNKRKPCHGINFVIAHDGFTLRDLVSYNVKHNDANGEGGNDGCNDNFSW 600

Query: 601 NCGFEGETEDTSIKALRSRQMKNFHLALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDNAL 660
           NCGFEGETEDTSIKALR+RQMKNFHLALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDNAL
Sbjct: 601 NCGFEGETEDTSIKALRTRQMKNFHLALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDNAL 660

Query: 661 NHFLWEQLEARKRDHFRFFSEVIKFRRKHPHLF-KDSF--QNDITWHESNWDNPESKFLA 720
           NHFLWEQLEARKRDHFRFFSEVIKFRRKHPHLF +++F  +NDITWHESNWDNPESKFLA
Sbjct: 661 NHFLWEQLEARKRDHFRFFSEVIKFRRKHPHLFSRENFLNKNDITWHESNWDNPESKFLA 720

Query: 721 YTLHDDNGEDVYLAFNAHDYFVNVSLPSPPTNRRWFRVVDTNLESPHDFVLDGIPGVGSS 777
           YTLHDDNGEDVYLAFNAH+YFVNVSLPSPPT R+WFRVVDTNLESPHDFVLDGIPGVGSS
Sbjct: 721 YTLHDDNGEDVYLAFNAHEYFVNVSLPSPPTKRKWFRVVDTNLESPHDFVLDGIPGVGSS 770

BLAST of IVF0022879 vs. NCBI nr
Match: XP_022936809.1 (isoamylase 3, chloroplastic isoform X1 [Cucurbita moschata])

HSP 1 Score: 1353 bits (3503), Expect = 0.0
Identity = 661/794 (83.25%), Postives = 696/794 (87.66%), Query Frame = 0

Query: 1   MIRSLPLHHSTTLQFPLFPSSCTGATHFLHFRLKRQVKLVSGSSSSDSREIFSETKQGGV 60
           MIRS+PLHHS  LQ PLF SSCTGA H + FRLK Q K   GSSSS S+E+FSETKQGGV
Sbjct: 1   MIRSIPLHHSAPLQLPLFASSCTGAAHSVQFRLKPQGKRAPGSSSSSSQEVFSETKQGGV 60

Query: 61  KLWTVSAFSRRDQDRVLEQEAPENLDTGPMLKIFPGQAFPLGVSEVDNGINFAIFSQHAT 120
           K+ TVSAF RRDQDRVLEQEAPENLDTGPMLK+ PGQAFPLGVSEVDNGINFAIFSQHAT
Sbjct: 61  KICTVSAFGRRDQDRVLEQEAPENLDTGPMLKVVPGQAFPLGVSEVDNGINFAIFSQHAT 120

Query: 121 SVTICLSLDGRIDDGMLEFKLDPDDNRTGDIWHICIQDLQRKNVLYGYRIDGPQGWHHGH 180
           +VTICLS + RIDD  LEFKLDPDDNRTGDIWHICIQDL RKNVLYGYRIDGPQGWHHGH
Sbjct: 121 AVTICLSFNERIDDRTLEFKLDPDDNRTGDIWHICIQDLPRKNVLYGYRIDGPQGWHHGH 180

Query: 181 RYDDGTMLLDPYAKFVEGRRFFGGENKSSGFLGTYDFESLPFDWGNDYKLPNIPEKDLVI 240
           R+DD  +LLDPYAKFVEGRR+FGGENKSSGFLGTYDF+SLPFDWG+DYKLPNI EKDLVI
Sbjct: 181 RFDDSIVLLDPYAKFVEGRRYFGGENKSSGFLGTYDFDSLPFDWGDDYKLPNILEKDLVI 240

Query: 241 YEMNVRGFTADESSGLPSSTRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHPN 300
           YEMNVR FTADESSGL SS RGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHPN
Sbjct: 241 YEMNVRAFTADESSGLASSIRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHPN 300

Query: 301 PRDHMINTWGYSTINFFAPMSRYASAGGGPLNASQEFKQMVKALHAAGIEVILDVVYNHT 360
           PR+HMINTWGYSTINFFAPMSRYASAGGGPLNAS+EFK+MVKALH AGIEVILDVVYNHT
Sbjct: 301 PRNHMINTWGYSTINFFAPMSRYASAGGGPLNASREFKEMVKALHGAGIEVILDVVYNHT 360

Query: 361 NEGDDVNPYTTSFRGIDNKVYYMLDLKNNGQYFNFSGCAT-----HPLVLQVYSGSLMIS 420
           NE DDV PYTTSFRGIDNKVYYMLDL+NNGQ+ NFSGC       HP+V+++   SL   
Sbjct: 361 NEADDVYPYTTSFRGIDNKVYYMLDLRNNGQFLNFSGCGNTLNCNHPVVMELILDSL--- 420

Query: 421 CNSTLIYYSTILNFRVVEYHVDGFRFDLASVLCRGTDGAPTYIRQVDMARDLNLLLFQAI 480
                          VVEYHVDGFRFDLASVLCRG DGAP               L +AI
Sbjct: 421 ------------RHWVVEYHVDGFRFDLASVLCRGPDGAPLSAPP----------LIRAI 480

Query: 481 SKDAILSRCKIIAEPWDCGGLIL---------WAEWNGIYRDDIRKFIKGDCGMKGSFAT 540
           SKDAILSRCKIIAEPWDCGGL L         WAEWNGIYRDDIRKFIKGDCGMKGSFAT
Sbjct: 481 SKDAILSRCKIIAEPWDCGGLYLVGRFPNWDRWAEWNGIYRDDIRKFIKGDCGMKGSFAT 540

Query: 541 RVAGSSDLYNVNKRKPYHGINFVIAHDGFTLRDLVSYNVKHNDANGEGGNDGCNDNFSWN 600
           RV+GSSDLY VN RKPYHGINFVIAHDGFTLRDLVSYNVKHN+ANGEGGNDGCNDNFSWN
Sbjct: 541 RVSGSSDLYKVNNRKPYHGINFVIAHDGFTLRDLVSYNVKHNNANGEGGNDGCNDNFSWN 600

Query: 601 CGFEGETEDTSIKALRSRQMKNFHLALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDNALN 660
           CGFEGETED +I+ALRSRQMKNFHLALMTSQGTPMMLMGDEYGHTRYGNNNSYGHD ALN
Sbjct: 601 CGFEGETEDANIRALRSRQMKNFHLALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDTALN 660

Query: 661 HFLWEQLEARKRDHFRFFSEVIKFRRKHPHLF-KDSF--QNDITWHESNWDNPESKFLAY 720
           HFLW QLEARKRDHFRFFSEVIKFRRKHPHLF +++F  +NDITWHESNWDNPESKFLAY
Sbjct: 661 HFLWGQLEARKRDHFRFFSEVIKFRRKHPHLFSRENFLGENDITWHESNWDNPESKFLAY 720

Query: 721 TLHDDNGEDVYLAFNAHDYFVNVSLPSPPTNRRWFRVVDTNLESPHDFVLDGIPGVGSSY 777
           TLHD NGEDVYLAFNAHDYFV+VSLPSPPTNR+WFRVVDTNLESP DFVLDGI G+GS+Y
Sbjct: 721 TLHDGNGEDVYLAFNAHDYFVSVSLPSPPTNRKWFRVVDTNLESPQDFVLDGIRGIGSTY 769

BLAST of IVF0022879 vs. NCBI nr
Match: XP_038896292.1 (isoamylase 3, chloroplastic isoform X1 [Benincasa hispida] >XP_038896293.1 isoamylase 3, chloroplastic isoform X1 [Benincasa hispida])

HSP 1 Score: 1352 bits (3499), Expect = 0.0
Identity = 666/794 (83.88%), Postives = 698/794 (87.91%), Query Frame = 0

Query: 1   MIRSLPLHHSTTLQFPLFPSSCTGATHFLHFRLKRQVKLVSGSSSSDSREIFSETKQGGV 60
           M+R LPLHHS +LQ PLF SSCT A + + FR K Q KL  GSSS  S++IFSE KQGGV
Sbjct: 1   MVRYLPLHHSASLQLPLFASSCTRAAYSVEFRSKPQGKLAPGSSSC-SQDIFSEIKQGGV 60

Query: 61  KLWTVSAFSRRDQDRVLEQEAPENLDTGPMLKIFPGQAFPLGVSEVDNGINFAIFSQHAT 120
           KL TV AF RRDQ+RVLEQEAPENLDTGPMLKIFPGQAFPLGVSEV +GINFAIFSQHAT
Sbjct: 61  KLCTVCAFGRRDQERVLEQEAPENLDTGPMLKIFPGQAFPLGVSEVADGINFAIFSQHAT 120

Query: 121 SVTICLSLDGRIDDGMLEFKLDPDDNRTGDIWHICIQDLQRKNVLYGYRIDGPQGWHHGH 180
           SVTICLSL+ RIDD MLEFKLDPD+NRTGDIWHIC QDL RK+VLYGYRIDGPQGWHHGH
Sbjct: 121 SVTICLSLNERIDDRMLEFKLDPDNNRTGDIWHICFQDLPRKSVLYGYRIDGPQGWHHGH 180

Query: 181 RYDDGTMLLDPYAKFVEGRRFFGGENKSSGFLGTYDFESLPFDWGNDYKLPNIPEKDLVI 240
           R+D  T+LLDPYAKFVEGRR+FGGENKSSGFLGTYDF+SLPFDWGNDYKLPNI EKDLVI
Sbjct: 181 RFDGSTVLLDPYAKFVEGRRYFGGENKSSGFLGTYDFDSLPFDWGNDYKLPNILEKDLVI 240

Query: 241 YEMNVRGFTADESSGLPSSTRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHPN 300
           YEMNVRGFTADESSGL SSTRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHPN
Sbjct: 241 YEMNVRGFTADESSGLASSTRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHPN 300

Query: 301 PRDHMINTWGYSTINFFAPMSRYASAGGGPLNASQEFKQMVKALHAAGIEVILDVVYNHT 360
           PRDHMIN WGYSTINFFAPMSRYASAGGGPLNAS+EFK+MVKALHAAGIEVILDVVYNHT
Sbjct: 301 PRDHMINMWGYSTINFFAPMSRYASAGGGPLNASREFKEMVKALHAAGIEVILDVVYNHT 360

Query: 361 NEGDDVNPYTTSFRGIDNKVYYMLDLKNNGQYFNFSGCAT-----HPLVLQVYSGSLMIS 420
           NE DDVNPYTTSFRGIDNKVYYMLDL+NNGQY NFSGC       HP+V+++   SL   
Sbjct: 361 NEADDVNPYTTSFRGIDNKVYYMLDLRNNGQYLNFSGCGNTLNCNHPVVMELILDSL--- 420

Query: 421 CNSTLIYYSTILNFRVVEYHVDGFRFDLASVLCRGTDGAPTYIRQVDMARDLNLLLFQAI 480
                          VV+YHVDGFRFDLASVLCRGTDGAP               L +AI
Sbjct: 421 ------------RHWVVDYHVDGFRFDLASVLCRGTDGAPLSAPP----------LIRAI 480

Query: 481 SKDAILSRCKIIAEPWDCGGLIL---------WAEWNGIYRDDIRKFIKGDCGMKGSFAT 540
           SKDAILSRCKIIAEPWDCGGL L         WAEWNGIYRDDIRKFIKGDCGMKGSFAT
Sbjct: 481 SKDAILSRCKIIAEPWDCGGLYLVGRFPNWDRWAEWNGIYRDDIRKFIKGDCGMKGSFAT 540

Query: 541 RVAGSSDLYNVNKRKPYHGINFVIAHDGFTLRDLVSYNVKHNDANGEGGNDGCNDNFSWN 600
           RVAGSSDLY VNKRKPYHGINFVIAHDGFTLRDLVSYNVKHND NGEGGNDG NDNFSWN
Sbjct: 541 RVAGSSDLYKVNKRKPYHGINFVIAHDGFTLRDLVSYNVKHNDVNGEGGNDGSNDNFSWN 600

Query: 601 CGFEGETEDTSIKALRSRQMKNFHLALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDNALN 660
           CGFEGETEDTSIKALR+RQMKNFHLALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDNALN
Sbjct: 601 CGFEGETEDTSIKALRTRQMKNFHLALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDNALN 660

Query: 661 HFLWEQLEARKRDHFRFFSEVIKFRRKHPHLF-KDSF--QNDITWHESNWDNPESKFLAY 720
           HFLW+QLEARK DHFRFFSEVIKFRRKHPHLF +++F  +NDI+WHESNWDNPESKFLAY
Sbjct: 661 HFLWDQLEARKTDHFRFFSEVIKFRRKHPHLFSRENFLSKNDISWHESNWDNPESKFLAY 720

Query: 721 TLHDDNGEDVYLAFNAHDYFVNVSLPSPPTNRRWFRVVDTNLESPHDFVLDGIPGVGSSY 777
           TLHDDNGEDVYLAFNAHDY VNVSLPSPPT RRWFRVVDTNLESPHDFVLDGIPGV S+Y
Sbjct: 721 TLHDDNGEDVYLAFNAHDYSVNVSLPSPPTKRRWFRVVDTNLESPHDFVLDGIPGVESTY 768

BLAST of IVF0022879 vs. TAIR 10
Match: AT4G09020.1 (isoamylase 3 )

HSP 1 Score: 1010.0 bits (2610), Expect = 1.0e-294
Identity = 491/735 (66.80%), Postives = 565/735 (76.87%), Query Frame = 0

Query: 64  TVSAFSRRDQDRVLEQEAPENLDTGPMLKIFPGQAFPLGVSEVDNGINFAIFSQHATSVT 123
           T+    RR  +RV+E+EA    +T  + K+  G+  PLGVS+VD GINFA+FSQ+ATSVT
Sbjct: 57  TLKVTCRRAHERVVEEEASTMTET-KLFKVSSGEVSPLGVSQVDKGINFALFSQNATSVT 116

Query: 124 ICLSL-----DGRIDDGMLEFKLDPDDNRTGDIWHICIQDLQRKNVLYGYRIDGPQGWHH 183
           +CLSL     D   DDGM+E  LDP  N+TGD WHIC++DL   NVLYGYR+DGP  W  
Sbjct: 117 LCLSLSQSGKDDTDDDGMIELVLDPSVNKTGDTWHICVEDLPLNNVLYGYRVDGPGEWQQ 176

Query: 184 GHRYDDGTMLLDPYAKFVEGRRFFG-GENKSSGFLGTYDFESLPFDWGNDYKLPNIPEKD 243
           GHR+D   +LLDPYAK V+G   FG    K + F GTYDFES PFDWG+DYK PNIPEKD
Sbjct: 177 GHRFDRSILLLDPYAKLVKGHSSFGDSSQKFAQFYGTYDFESSPFDWGDDYKFPNIPEKD 236

Query: 244 LVIYEMNVRGFTADESSGLPSSTRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQR 303
           LVIYEMNVR FTADESSG+  +  GSYLG IEKIPHL +LG+NAVELLPVFEFDELE QR
Sbjct: 237 LVIYEMNVRAFTADESSGMDPAIGGSYLGFIEKIPHLQDLGINAVELLPVFEFDELELQR 296

Query: 304 HPNPRDHMINTWGYSTINFFAPMSRYASAGGGPLNASQEFKQMVKALHAAGIEVILDVVY 363
             NPRDHM+NTWGYST+NFFAPMSRYAS  G P+ AS+EFK+MVKALH+AGIEVILDVVY
Sbjct: 297 RSNPRDHMVNTWGYSTVNFFAPMSRYASGEGDPIKASKEFKEMVKALHSAGIEVILDVVY 356

Query: 364 NHTNEGDDVNPYTTSFRGIDNKVYYMLDLKNNGQYFNFSGC-----ATHPLVLQVYSGSL 423
           NHTNE DD  PYTTSFRGIDNKVYYMLD   N Q  NFSGC       HP+V+++   S 
Sbjct: 357 NHTNEADDKYPYTTSFRGIDNKVYYMLD--PNNQLLNFSGCGNTLNCNHPVVMELILDS- 416

Query: 424 MISCNSTLIYYSTILNFRVVEYHVDGFRFDLASVLCRGTDGAPTYIRQVDMARDLNLLLF 483
                         L   V EYHVDGFRFDLASVLCR TDG+P               L 
Sbjct: 417 --------------LRHWVTEYHVDGFRFDLASVLCRATDGSPLSAPP----------LI 476

Query: 484 QAISKDAILSRCKIIAEPWDCGGLIL---------WAEWNGIYRDDIRKFIKGDCGMKGS 543
           +AI+KD++LSRCKIIAEPWDCGGL L         WAEWNG+YRDD+R+FIKGD GMKGS
Sbjct: 477 RAIAKDSVLSRCKIIAEPWDCGGLYLVGKFPNWDRWAEWNGMYRDDVRRFIKGDSGMKGS 536

Query: 544 FATRVAGSSDLYNVNKRKPYHGINFVIAHDGFTLRDLVSYNVKHNDANGEGGNDGCNDNF 603
           FATRV+GSSDLY VN+RKPYHG+NFVIAHDGFTLRDLVSYN KHN+ANGEGGNDGCNDN 
Sbjct: 537 FATRVSGSSDLYQVNQRKPYHGVNFVIAHDGFTLRDLVSYNFKHNEANGEGGNDGCNDNH 596

Query: 604 SWNCGFEGETEDTSIKALRSRQMKNFHLALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDN 663
           SWNCGFEGET D  IK+LR+RQMKNFHLALM SQGTPMMLMGDEYGHTRYGNNNSYGHD 
Sbjct: 597 SWNCGFEGETGDAHIKSLRTRQMKNFHLALMISQGTPMMLMGDEYGHTRYGNNNSYGHDT 656

Query: 664 ALNHFLWEQLEARKRDHFRFFSEVIKFRRKHPHLFKDSF--QNDITWHESNWDNPESKFL 723
           +LN+F W++L+A+K++HFRFFSEVIKFR  H  L  ++F  Q +ITWHE NWDN ESKFL
Sbjct: 657 SLNNFQWKELDAKKQNHFRFFSEVIKFRHSHHVLKHENFLTQGEITWHEDNWDNSESKFL 716

Query: 724 AYTLHDD-NGEDVYLAFNAHDYFVNVSLPSPPTNRRWFRVVDTNLESPHDFVLDGIPGVG 776
           A+TLHD   G D+Y+AFNAHDYFV   +P PP  ++WFRV DTNLESP DFV +G+ GV 
Sbjct: 717 AFTLHDGIGGRDIYVAFNAHDYFVKALIPQPPPGKQWFRVADTNLESPDDFVREGVAGVA 763

BLAST of IVF0022879 vs. TAIR 10
Match: AT2G39930.1 (isoamylase 1 )

HSP 1 Score: 542.3 bits (1396), Expect = 6.1e-154
Identity = 331/765 (43.27%), Postives = 435/765 (56.86%), Query Frame = 0

Query: 64  TVSAFSRR--DQDRVLEQEAPENLDTGPMLKIFPGQAFPLGVSEVDNGINFAIFSQHATS 123
           ++SA  RR  + + +   E P   D      I  G   P G +  D+G+NF+++S ++ S
Sbjct: 41  SISAKDRRSNEAENIAVVEKPLKSD---RFFISDGLPSPFGPTVRDDGVNFSVYSTNSVS 100

Query: 124 VTICL-SL-DGRIDDGMLEFKLDPDDNRTGDIWHICIQDLQRKNVLYGYRIDGPQGWHHG 183
            TICL SL D R +    E +LDP  NRTG +WH+ ++    K++LYGYR DG      G
Sbjct: 101 ATICLISLSDLRQNKVTEEIQLDPSRNRTGHVWHVFLRG-DFKDMLYGYRFDGKFSPEEG 160

Query: 184 HRYDDGTMLLDPYAKFVEGRRFFG----GENKSSGFLGTYDFESLPFDWGNDYKLPNIPE 243
           H YD   +LLDPYAK +  R  FG     +N               FDW  D  L  +P+
Sbjct: 161 HYYDSSNILLDPYAKAIISRDEFGVLGPDDNCWPQMACMVPTREEEFDWEGDMHL-KLPQ 220

Query: 244 KDLVIYEMNVRGFTADESSGLPSSTRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEF 303
           KDLVIYEM+VRGFT  ESS +     G+Y GV EK+ HL ELG+N +EL+P  EF+ELE+
Sbjct: 221 KDLVIYEMHVRGFTRHESSKI--EFPGTYQGVAEKLDHLKELGINCIELMPCHEFNELEY 280

Query: 304 QRHPN-PRDHMINTWGYSTINFFAPMSRYASAGGGPL--NASQEFKQMVKALHAAGIEVI 363
             +     DH +N WGYSTI FF+PM RYASA        A  EFK +VK  H  GIEVI
Sbjct: 281 YSYNTILGDHRVNFWGYSTIGFFSPMIRYASASSNNFAGRAINEFKILVKEAHKRGIEVI 340

Query: 364 LDVVYNHTNEGDDVNPYTTSFRGIDNKVYYMLDLKNNGQYFNFSGC-----ATHPLVLQV 423
           +DVV NHT EG++  P   SFRG+DN VYYML  K  G+++N+SGC       HP+V Q 
Sbjct: 341 MDVVLNHTAEGNEKGP-IFSFRGVDNSVYYMLAPK--GEFYNYSGCGNTFNCNHPVVRQ- 400

Query: 424 YSGSLMISCNSTLIYYSTILNFRVVEYHVDGFRFDLASVLCRGT---DGAPTYIRQVDMA 483
                ++ C          L + V E HVDGFRFDL S++ R +   D A  Y    D+ 
Sbjct: 401 ----FILDC----------LRYWVTEMHVDGFRFDLGSIMSRSSSLWDAANVY--GADVE 460

Query: 484 RDLNLL--------LFQAISKDAILSRCKIIAEPWDCGGLI---------LWAEWNGIYR 543
            DL           +   IS D IL   K+IAE WD GGL          +W+EWNG +R
Sbjct: 461 GDLLTTGTPISCPPVIDMISNDPILRGVKLIAEAWDAGGLYQVGMFPHWGIWSEWNGKFR 520

Query: 544 DDIRKFIKGDCGMKGSFATRVAGSSDLYNVNKRKPYHGINFVIAHDGFTLRDLVSYNVKH 603
           D +R+FIKG  G  G+FA  + GS +LY    RKP+H INF+ AHDGFTL DLV+YN K+
Sbjct: 521 DVVRQFIKGTDGFSGAFAECLCGSPNLYQ-GGRKPWHSINFICAHDGFTLADLVTYNNKN 580

Query: 604 NDANGEGGNDGCNDNFSWNCGFEGETEDTSIKALRSRQMKNFHLALMTSQGTPMMLMGDE 663
           N ANGE  NDG N N+SWNCG EG+    S+K LR RQM+NF ++LM SQG PM+ MGDE
Sbjct: 581 NLANGEENNDGENHNYSWNCGEEGDFASISVKRLRKRQMRNFFVSLMVSQGVPMIYMGDE 640

Query: 664 YGHTRYGNNNSYGHDNALNHFLWEQLEARKRDHFRFFSEVIKFRRKHPHLFKDSF--QND 723
           YGHT+ GNNN+Y HDN +N+F W++ E    D FRF   +IKFR +   L  + F     
Sbjct: 641 YGHTKGGNNNTYCHDNYMNYFRWDKKEEAHSDFFRFCRILIKFRDECESLGLNDFPTAKR 700

Query: 724 ITWHE-----SNWDNPESKFLAYTLHDDNGEDVYLAFNAHDYFVNVSLPSPPTNRRWFRV 774
           + WH       NW +  S+F+A++L D   +++Y+AFN       VSLP+ P   RW   
Sbjct: 701 LQWHGLAPEIPNW-SETSRFVAFSLVDSVKKEIYVAFNTSHLATLVSLPNRP-GYRWEPF 760

BLAST of IVF0022879 vs. TAIR 10
Match: AT1G03310.2 (debranching enzyme 1 )

HSP 1 Score: 308.9 bits (790), Expect = 1.1e-83
Identity = 235/726 (32.37%), Postives = 344/726 (47.38%), Query Frame = 0

Query: 96  GQAFPLGVSE--VDNGINFAIFSQHATSVTICLSLDGRIDDGMLEFKLDPDDNRTGDIWH 155
           G   PLG+S    D+  NF+ FS+ +T+V +CL  D   D   LE  LDP  NRTGD+WH
Sbjct: 230 GHPLPLGLSSGPDDDSWNFSFFSRSSTNVVLCLYDDSTTDKPALELDLDPYVNRTGDVWH 289

Query: 156 ICIQDLQRKNVLYGYRIDGPQGWHHGHRYDDGTMLLDPYAKFVEGRRFFGGENKSSGFLG 215
             + D     V YGYR             +   ++LDPYA  V       G++ S  +LG
Sbjct: 290 ASV-DNTWDFVRYGYRCKETAHSKEDVDVEGEPIVLDPYATVV-------GKSVSQKYLG 349

Query: 216 TYDFESLPFDWGNDYKLPNIPEKDLVIYEMNVRGFTADESSGLPSSTRGSYLGVIEKIPH 275
           +   +S  FDWG D   PNIP + L++Y +NV+GFT   SS LPS+  G++ GV EK+ H
Sbjct: 350 SLS-KSPSFDWGEDVS-PNIPLEKLLVYRLNVKGFTQHRSSKLPSNVAGTFSGVAEKVSH 409

Query: 276 LLELGVNAVELLPVFEFDELEFQRHPNPRDHMINTWGYSTINFFAPMSRYASAGGGPLN- 335
           L  LG NAV L P+F F E   Q+ P           Y   +FF+PM  Y     GP N 
Sbjct: 410 LKTLGTNAVLLEPIFSFSE---QKGP-----------YFPFHFFSPMDIY-----GPSNS 469

Query: 336 ---ASQEFKQMVKALHAAGIEVILDVVYNHTNEGDDVNPYTTSFRGIDNKVYYMLDLKNN 395
              A    K MVK LH+ GIEV+L+VV+ HT +       + + RGID+  YY     N+
Sbjct: 470 LESAVNSMKVMVKKLHSEGIEVLLEVVFTHTAD-------SGALRGIDDSSYYYKGRAND 529

Query: 396 GQYFNFSGCATHPLVLQVYSGSLMISCNSTLIYYSTILNFRVVEYHVDGFRFDLASVLCR 455
               ++  C  +P+V Q+   S               L + V E+HVDGF F  AS L R
Sbjct: 530 LDSKSYLNC-NYPVVQQLVLES---------------LRYWVTEFHVDGFCFINASSLLR 589

Query: 456 GTDGAPTYIRQVDMARDLNLLLFQAISKDAILSRCKIIAEPWDCGGLI----------LW 515
           G  G         ++R     L +AI+ D +L+  K+IA+ WD   ++           W
Sbjct: 590 GVHGE-------QLSRP---PLVEAIAFDPLLAETKLIADCWDPLEMMPKEVRFPHWKRW 649

Query: 516 AEWNGIYRDDIRKFIKGDCGMKGSFATRVAGSSDLYNVNKRKPYHGINFVIAHDGFTLRD 575
           AE N  Y  ++R F++G  G+    ATR+ GS D++  + R P    N++  + G +L D
Sbjct: 650 AELNTRYCRNVRNFLRGR-GVLSDLATRICGSGDVF-TDGRGPAFSFNYISRNSGLSLVD 709

Query: 576 LVSYNVKHNDANGEGGNDGCNDNFSWNCGFEGETEDTSIKALRSRQMKNFHLALMTSQGT 635
           +VS++                   SWNCG EG T  +++   R +Q++NF      S G 
Sbjct: 710 IVSFS-----------GPELASELSWNCGEEGATNKSAVLQRRLKQIRNFLFIQYISLGV 769

Query: 636 PMMLMGDEYGHTRYGNNNSYGHDNALNHFLWEQLEA---RKRDHFRFFSEVIKFRRKHPH 695
           P++ MGDE G +  G+        +   F W  L +    +   F  F   ++ RR    
Sbjct: 770 PVLNMGDECGISTRGSPLL----ESRKPFDWNLLASAFGTQITQFISFMTSVRARRSDVF 829

Query: 696 LFKDSFQ-NDITWHESN-----WDNPESKFLAYTLHDDNGE---------------DVYL 755
             +D  +  +I W+ ++     W++P SKFLA  +  ++ E               D+++
Sbjct: 830 QRRDFLKPENIVWYANDQTTPKWEDPASKFLALEIKSESEEEETASLAEPNEPKSNDLFI 876

Query: 756 AFNAHDYFVNVSLPSPPTNRRWFRVVDTNLESPHDFVLDGIPGVGSS-------YNLAPY 775
            FNA D+  +V LPS P   +W R+VDT L  P  F ++G   V          Y + PY
Sbjct: 890 GFNASDHPESVVLPSLPDGSKWRRLVDTALPFPGFFSVEGETVVAEEPLQQLVVYEMKPY 876

BLAST of IVF0022879 vs. TAIR 10
Match: AT1G03310.1 (debranching enzyme 1 )

HSP 1 Score: 308.9 bits (790), Expect = 1.1e-83
Identity = 235/726 (32.37%), Postives = 344/726 (47.38%), Query Frame = 0

Query: 96  GQAFPLGVSE--VDNGINFAIFSQHATSVTICLSLDGRIDDGMLEFKLDPDDNRTGDIWH 155
           G   PLG+S    D+  NF+ FS+ +T+V +CL  D   D   LE  LDP  NRTGD+WH
Sbjct: 230 GHPLPLGLSSGPDDDSWNFSFFSRSSTNVVLCLYDDSTTDKPALELDLDPYVNRTGDVWH 289

Query: 156 ICIQDLQRKNVLYGYRIDGPQGWHHGHRYDDGTMLLDPYAKFVEGRRFFGGENKSSGFLG 215
             + D     V YGYR             +   ++LDPYA  V       G++ S  +LG
Sbjct: 290 ASV-DNTWDFVRYGYRCKETAHSKEDVDVEGEPIVLDPYATVV-------GKSVSQKYLG 349

Query: 216 TYDFESLPFDWGNDYKLPNIPEKDLVIYEMNVRGFTADESSGLPSSTRGSYLGVIEKIPH 275
           +   +S  FDWG D   PNIP + L++Y +NV+GFT   SS LPS+  G++ GV EK+ H
Sbjct: 350 SLS-KSPSFDWGEDVS-PNIPLEKLLVYRLNVKGFTQHRSSKLPSNVAGTFSGVAEKVSH 409

Query: 276 LLELGVNAVELLPVFEFDELEFQRHPNPRDHMINTWGYSTINFFAPMSRYASAGGGPLN- 335
           L  LG NAV L P+F F E   Q+ P           Y   +FF+PM  Y     GP N 
Sbjct: 410 LKTLGTNAVLLEPIFSFSE---QKGP-----------YFPFHFFSPMDIY-----GPSNS 469

Query: 336 ---ASQEFKQMVKALHAAGIEVILDVVYNHTNEGDDVNPYTTSFRGIDNKVYYMLDLKNN 395
              A    K MVK LH+ GIEV+L+VV+ HT +       + + RGID+  YY     N+
Sbjct: 470 LESAVNSMKVMVKKLHSEGIEVLLEVVFTHTAD-------SGALRGIDDSSYYYKGRAND 529

Query: 396 GQYFNFSGCATHPLVLQVYSGSLMISCNSTLIYYSTILNFRVVEYHVDGFRFDLASVLCR 455
               ++  C  +P+V Q+   S               L + V E+HVDGF F  AS L R
Sbjct: 530 LDSKSYLNC-NYPVVQQLVLES---------------LRYWVTEFHVDGFCFINASSLLR 589

Query: 456 GTDGAPTYIRQVDMARDLNLLLFQAISKDAILSRCKIIAEPWDCGGLI----------LW 515
           G  G         ++R     L +AI+ D +L+  K+IA+ WD   ++           W
Sbjct: 590 GVHGE-------QLSRP---PLVEAIAFDPLLAETKLIADCWDPLEMMPKEVRFPHWKRW 649

Query: 516 AEWNGIYRDDIRKFIKGDCGMKGSFATRVAGSSDLYNVNKRKPYHGINFVIAHDGFTLRD 575
           AE N  Y  ++R F++G  G+    ATR+ GS D++  + R P    N++  + G +L D
Sbjct: 650 AELNTRYCRNVRNFLRGR-GVLSDLATRICGSGDVF-TDGRGPAFSFNYISRNSGLSLVD 709

Query: 576 LVSYNVKHNDANGEGGNDGCNDNFSWNCGFEGETEDTSIKALRSRQMKNFHLALMTSQGT 635
           +VS++                   SWNCG EG T  +++   R +Q++NF      S G 
Sbjct: 710 IVSFS-----------GPELASELSWNCGEEGATNKSAVLQRRLKQIRNFLFIQYISLGV 769

Query: 636 PMMLMGDEYGHTRYGNNNSYGHDNALNHFLWEQLEA---RKRDHFRFFSEVIKFRRKHPH 695
           P++ MGDE G +  G+        +   F W  L +    +   F  F   ++ RR    
Sbjct: 770 PVLNMGDECGISTRGSPLL----ESRKPFDWNLLASAFGTQITQFISFMTSVRARRSDVF 829

Query: 696 LFKDSFQ-NDITWHESN-----WDNPESKFLAYTLHDDNGE---------------DVYL 755
             +D  +  +I W+ ++     W++P SKFLA  +  ++ E               D+++
Sbjct: 830 QRRDFLKPENIVWYANDQTTPKWEDPASKFLALEIKSESEEEETASLAEPNEPKSNDLFI 876

Query: 756 AFNAHDYFVNVSLPSPPTNRRWFRVVDTNLESPHDFVLDGIPGVGSS-------YNLAPY 775
            FNA D+  +V LPS P   +W R+VDT L  P  F ++G   V          Y + PY
Sbjct: 890 GFNASDHPESVVLPSLPDGSKWRRLVDTALPFPGFFSVEGETVVAEEPLQQLVVYEMKPY 876

BLAST of IVF0022879 vs. TAIR 10
Match: AT5G04360.1 (limit dextrinase )

HSP 1 Score: 92.8 bits (229), Expect = 1.3e-18
Identity = 99/394 (25.13%), Postives = 161/394 (40.86%), Query Frame = 0

Query: 100 PLGVSEVDNGINFAIFSQHATSVTICLSLDGRIDDGMLEFKLDPDDNRTGDIWHICIQDL 159
           PLG      G++  +++  A +V++C+     +D   +E     + N        C    
Sbjct: 207 PLGAHFTPEGVSLHLWAPTAQAVSVCI-YKNPLDKSPMEICPLKEANGVWSTEGAC---- 266

Query: 160 QRKNVLYGYRIDGPQGWHHGHRYDDGTMLL------DPYAK--FVEGRRFFGGENKSSGF 219
                 Y Y++           Y   TM L      DPYA+    +GR+          F
Sbjct: 267 SWGGCYYVYKVS---------VYHPSTMKLETCYANDPYARGLSADGRK---------TF 326

Query: 220 LGTYDFESL-PFDWGN--DYKLPNIPEKDLVIYEMNVRGFTADESSGLPSSTRGSYLGVI 279
           L   D + L P  W N  D K       D+ IYE++VR F+A++ +  P + RG YL   
Sbjct: 327 LVNLDSDDLKPEGWDNLADKKPCLRSFSDISIYELHVRDFSANDETVEPEN-RGGYLAFT 386

Query: 280 EK-------IPHLLELGVNAVELLPVFEFDELEFQR--------------HPNPRDHMIN 339
            K       +  L++ G+  + LLP F+F +++ ++               P+  +    
Sbjct: 387 SKDSAGVKHLQKLVDAGLTHLHLLPTFQFGDVDDEKENWKSVDTSLLEGLRPDSTEAQAR 446

Query: 340 ----------TWGYSTINFFAPMSRYASAGGGPLNASQEFKQMVKALHAAGIEVILDVVY 399
                      WGY+ + +  P   YAS   GP     EF++MV+AL+  G+ V+LDVVY
Sbjct: 447 ITEIQNDDGYNWGYNPVLWGVPKGSYASDPTGPCRII-EFRKMVQALNCTGLNVVLDVVY 506

Query: 400 NHTNEGDDVNPYTTSFRGIDNKVYYMLDLKNNGQYFNFSGCATHPLVLQVYSGSLMISCN 452
           NH +      P+      + +K+     L+ N   F  +    +    + Y    +I   
Sbjct: 507 NHLHAS---GPHDK--ESVLDKIVPGYYLRRNSDGFIENSTCVNNTASEHYMVDRLIR-- 561