IVF0022875 (gene) Melon (IVF77) v1

Overview
NameIVF0022875
Typegene
OrganismCucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
DescriptionCysteine proteinase inhibitor
Locationchr10: 4427396 .. 4431501 (+)
RNA-Seq ExpressionIVF0022875
SyntenyIVF0022875
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AGAAGATAGAGTTGATGTCTTGAATGCAATGCGTTGACAGTTCAATTAAACCTATTGAGAATCCAAAAGAAATTTGATTCTTTGCCCAATAATAAATAAAATGCTTAAACCAAACGCATCGCTTCGTGGCGCAGTTACCTAGTTGGCACGAATCCCATTTTACGATCGTGGCGCCAAGAGAGGGAGAGAGCGATTCTCATTCATATCTCACCGCGAAGAAGTTTTTCACAAAATCGAAAGAGATGAAAGGAACTCTGGAAATCTCTCCTCTGTCATGAACAATCATTCCACCATTTCGTTTTCTCCACTATCATCTACTTTGTCGTTGTTGTTGTTGCTGCTGTTTTTCTCTTGGATCATCGTTCTTACAGGTAACGCATCAGCTCACTTTTGCGCTGAGCAGGACGATCCACTCCTTATGGCGTCCACACTTGGAGGCGTTCACGATTCTCAGGGAGCTTCCAACTCCACCGATGTCGATGAACTCGCTCGTTTTGCCGTCGAGGAACACAACAAGAAAGAGGTAAACAGCTTCTCCTTCGATATCCGTCTGTTTAAATGAATTTTGTTTCTTACTCGGTGTTTTTTATCCTGTCGCGTTTCGTTGATTCTCGCTGATGGCGTCTGTGCCTTTTGTTGTCGATCTCGTTCTTGCTGTGTGTTGTGCTGTGGATTTCTGTGTTTCAGTCAGAATTCATTTCGCGATGTTGAATTTTGTTCGACTTGTTCTGCATTTTGATGCTGTCGGTGTCTGTCGGTGTCTGTCGGTGTCGATGAGTAGTATGAGGGTGTGTGTTGAACTAAAACTGCACGATTCTCTTCGTCTTTCTTTGTTTGGTAGATTGGATCGGTTGACTGTGGTGTGATTCTTATTTCTTTAAGGAACTTGACTGTCTATAGTTTTCAGGTTTGGTCAGTGGATATGCACATAATGTGTCTTTAGATTGATCATAGAATTTGAATTCGCATCTAAAAGCTTGAAAAACTGAACCAGTCTTTCTGGCTATAAATAGTTAGGATTAACATTCAATTTTGATTATGTAAATCTTGCAATCCTCCATAAGATGATAACTGGAAACATGTTGGCGTTTTAATCTTTTCAAGGCTAATGGAGACCATGTTAGCTTGCCCCTGTGACGATGTGATTTCTATTTTTCTGATCACTTCTAGTTACTTAAACATGCACGTTTCCATGTCTTCAGAAAAGAAGTTTGTTTCTTCGTTTCTCACAAGTGTTTTATGTTAATTTTATTTCAGTCTAAACACTCTTTCTATGAAATTCCAACTAATTGTATATTGATTTTATTTTAGTATAACAATTCCTTTTACTATCAAATCCAATGTATATGTATACTAACTGCATTTCAGTTTAAAAGAGTTATTTTTCTCTCATTTCTGAGTATTTGTATTGAAGTTTTATTCCAACTTTTAAGTGGATGTTCTATTTTGATTTCACGGGCTCTTTTTCCATAATTAAGGAGGAAATTTTCCTTTGACAAATATCCACGATAATTTGTTGGAAATTTCCAAATTGGATACACATAAGCAAAGTATTGTAGTTTTTTGCAACTAAAAATTCCTCCTGTTCTTATTTCCACTTTTCATGCAGCTGACTTATACTTTTCACTTGTTATATATGTGTATGACGTAATTATCTTCCCATTCCTTTGCAGAATTCACTTCTCGAGTTTGTGAGAGTCGTGAAGGCGAAAGAGCAGGTAGTTGCTGGTACACTACACCATCTTACTGTTGAGGTGGTTGAGGCTGGTAAAAAGAAGCTGTATGAAGCTAAGGTTTGGGTGAAGTCATGGATGAATTTTAAAGAATTGCAAGAGTTTAAGCATGCAGGCGATGTCCCCTCAATTTCTCCTTCTGATCTTGGTGCAAAAAAGGTGGATAACATTCTTTAACTTCTGCAGTGCAGTTTCAATTACAATTTAAAATAGCTTTGAATATATAACCAATGTCAAAGGAAATGTGTTGACTCATATTCTTCCTGTTTTTTGGAGATCACTTTCTGTTGAAGTCATTCATAACCTCTTGAAGGCTGATAAAATCTGAAGTTGTGGCTTTGTTTCTTTTCTAATTTACTAAGGGTTGATATTCTTGCAAACTTGCATCGTCCACTATTTAGCAAATGTTAGTAGCTCTATTATAGAGCATAAGCATTCATTAGTTCTTGTATGTTCGTTTGTATTCTTACAGGTCATAAAGCTGTAAATGTATGTGAAAGTCTTTTAGCTGATTTTACAGAGGGCGATTGTTTTCTTTAATATACTATTGACCACGGTTAGAAATTAGGTGATCATCCCCAGGGATGGCGTGAAGTGCCACCACATGATCCTCATGTTCAGGATGCAGCACAGCATGCTCTTCGAACGATCCAGCAGAGATCTAATTCTCTAGTCCCGTATGAACTGCTGGAGATCATTCATGCCAAAGCAGAGGTAAATGGATTATGGTCTTTTTATGTAGTATGTTTTACCTCTATTTAAGTTGGTTATCACGAGACTGTGAAGTTGATCTATGATATCAGGATTTCTCTGTCCTTTTCTTTCTTGCGTAATTGAAACTTTTTCATTGATGATGAAATAGGACTAAACTCAAATTATTGAAAGGTGAATCATAAAAGAGCTCTCCAACTAGAAATCAACAAAGAAAGACTTTAGTCATTAAAAGGCTGTTTACATTGACACCAATACAAATAAAACGTATAAAATGAAATCAATTCCACAAAGCTAAATTCTTCCATTCCTCTTGCTCCAAAGAGGCCAAGGAATTTTTGTTTGTGGCAAACCCCACCGTGGAAAGGATGTTCCACAGAGACGAAAGCGTGATTATCCTAAATGTTTGGAAAAGACATGAAACATGTGTATGTCTGGGTTAGAAAAACACATCAAGATAGAGAGAGGTTGGAATGAGGCTTTCTTTTTAGAGAATGAAATAGCTCCAAGACTTAGCTTCGCGTAGAAAGATCTTTATTTATTTATTCCTTTTCTTTTTCTTTTTTTTTTTTAGAAACATGGAAGAAAACAAGGGCATATAAAAAACTGAATCTGCAAAATTCAATCCAACTAGATAAAACGTTACCTAAAACATCTTTATATTCTTTGTTCCTTTATAATTTAGACATGTATGATCGACCAGACAGTAAAGGTAGCATTCATCCCAGAAGGCATTGAGTTTGAACTTTTGTAGTATTCACTGCGTTTCTATGTTAATGTCATTTTGATTAGCTTAGCTTCTAATCTACCACTTCTATCAAACTCAATATGACCAACATTTTAAACCCGAGAAGGTAGGCATTCATTCATACCCCATGTTCATACTTTATGAAATTTTTCTTCTGGTGAAGACTCAACATCCTAAATCGCATACCTTGCGTTCATTTTAATTATGTTATTTATCAATTTACATAATACTAATGGAGAGATTGACGATGGTGTGAGAACAGGTGATCGAAGATGCTGCGAAGTTTGATTTGCTCCTAAAGCTGAAGAGAGGGAGTAAAGAAGAGAAGTTCAAAGTGGAGGTCCACAAGAACAATGAAGGTAACTTCCTTCTAAATCAGATGGTGCAAGATCATTCCTAAAAGAGTTTGGATTGTTCAATATAATTAAATAAAATACAAGTTTATGTACGATAGAACCTTTAAAGCTGTATACTTCATAACTTCTTTTGAAGCTCGAGTCGTCCGGCTGATGCATAAAAAGACCATGATAAATGATGTAGCCACTATTTAATTAGGTTTTGGATCTGTGGTTTCCTTTTCTTCTGTTTTCTTTAATCCTTTCTTGAATTGTATGAACAACAAAACGTAGGGATCGTGGGTATACTCTCGATTCTGGGTTTACACTATAATTTGCTTCGTATGAAGTGAACCTAGAAGGGTTTCTTAGAGGTCTTTTTTGTTTTCGACATAGTGTTAGGAGCTCGTTTAAAAACAGCAAACAGATGGGAGTTAATGTGGATTTAAGCTTAATTTGGGATTGTAAATTAGTATCATGTGGTGTGTTTATTAATTTTAAACCATAGAAATCACCTTGTTTGGCGACTGGCAAATGATAATCCAGATTGGCGTGATTTAGCTATTGGACGTTATAGGG

mRNA sequence

AGAAGATAGAGTTGATGTCTTGAATGCAATGCGTTGACAGTTCAATTAAACCTATTGAGAATCCAAAAGAAATTTGATTCTTTGCCCAATAATAAATAAAATGCTTAAACCAAACGCATCGCTTCGTGGCGCAGTTACCTAGTTGGCACGAATCCCATTTTACGATCGTGGCGCCAAGAGAGGGAGAGAGCGATTCTCATTCATATCTCACCGCGAAGAAGTTTTTCACAAAATCGAAAGAGATGAAAGGAACTCTGGAAATCTCTCCTCTGTCATGAACAATCATTCCACCATTTCGTTTTCTCCACTATCATCTACTTTGTCGTTGTTGTTGTTGCTGCTGTTTTTCTCTTGGATCATCGTTCTTACAGGTAACGCATCAGCTCACTTTTGCGCTGAGCAGGACGATCCACTCCTTATGGCGTCCACACTTGGAGGCGTTCACGATTCTCAGGGAGCTTCCAACTCCACCGATGTCGATGAACTCGCTCGTTTTGCCGTCGAGGAACACAACAAGAAAGAGAATTCACTTCTCGAGTTTGTGAGAGTCGTGAAGGCGAAAGAGCAGGTAGTTGCTGGTACACTACACCATCTTACTGTTGAGGTGGTTGAGGCTGGTAAAAAGAAGCTGTATGAAGCTAAGGTTTGGGTGAAGTCATGGATGAATTTTAAAGAATTGCAAGAGTTTAAGCATGCAGGCGATGTCCCCTCAATTTCTCCTTCTGATCTTGGTGCAAAAAAGGTGATCATCCCCAGGGATGGCGTGAAGTGCCACCACATGATCCTCATGTTCAGGATGCAGCACAGCATGCTCTTCGAACGATCCAGCAGAGATCTAATTCTCTAGTCCCGTATGAACTGCTGGAGATCATTCATGCCAAAGCAGAGGTGATCGAAGATGCTGCGAAGTTTGATTTGCTCCTAAAGCTGAAGAGAGGGAGTAAAGAAGAGAAGTTCAAAGTGGAGGTCCACAAGAACAATGAAGGTAACTTCCTTCTAAATCAGATGGTGCAAGATCATTCCTAAAAGAGTTTGGATTGTTCAATATAATTAAATAAAATACAAGTTTATGTACGATAGAACCTTTAAAGCTGTATACTTCATAACTTCTTTTGAAGCTCGAGTCGTCCGGCTGATGCATAAAAAGACCATGATAAATGATGTAGCCACTATTTAATTAGGTTTTGGATCTGTGGTTTCCTTTTCTTCTGTTTTCTTTAATCCTTTCTTGAATTGTATGAACAACAAAACGTAGGGATCGTGGGTATACTCTCGATTCTGGGTTTACACTATAATTTGCTTCGTATGAAGTGAACCTAGAAGGGTTTCTTAGAGGTCTTTTTTGTTTTCGACATAGTGTTAGGAGCTCGTTTAAAAACAGCAAACAGATGGGAGTTAATGTGGATTTAAGCTTAATTTGGGATTGTAAATTAGTATCATGTGGTGTGTTTATTAATTTTAAACCATAGAAATCACCTTGTTTGGCGACTGGCAAATGATAATCCAGATTGGCGTGATTTAGCTATTGGACGTTATAGGG

Coding sequence (CDS)

ATGAACAATCATTCCACCATTTCGTTTTCTCCACTATCATCTACTTTGTCGTTGTTGTTGTTGCTGCTGTTTTTCTCTTGGATCATCGTTCTTACAGGTAACGCATCAGCTCACTTTTGCGCTGAGCAGGACGATCCACTCCTTATGGCGTCCACACTTGGAGGCGTTCACGATTCTCAGGGAGCTTCCAACTCCACCGATGTCGATGAACTCGCTCGTTTTGCCGTCGAGGAACACAACAAGAAAGAGAATTCACTTCTCGAGTTTGTGAGAGTCGTGAAGGCGAAAGAGCAGGTAGTTGCTGGTACACTACACCATCTTACTGTTGAGGTGGTTGAGGCTGGTAAAAAGAAGCTGTATGAAGCTAAGGTTTGGGTGAAGTCATGGATGAATTTTAAAGAATTGCAAGAGTTTAAGCATGCAGGCGATGTCCCCTCAATTTCTCCTTCTGATCTTGGTGCAAAAAAGGTGATCATCCCCAGGGATGGCGTGAAGTGCCACCACATGATCCTCATGTTCAGGATGCAGCACAGCATGCTCTTCGAACGATCCAGCAGAGATCTAATTCTCTAG

Protein sequence

MNNHSTISFSPLSSTLSLLLLLLFFSWIIVLTGNASAHFCAEQDDPLLMASTLGGVHDSQGASNSTDVDELARFAVEEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLYEAKVWVKSWMNFKELQEFKHAGDVPSISPSDLGAKKVIIPRDGVKCHHMILMFRMQHSMLFERSSRDLIL
Homology
BLAST of IVF0022875 vs. ExPASy Swiss-Prot
Match: Q8H0X6 (Cysteine proteinase inhibitor 6 OS=Arabidopsis thaliana OX=3702 GN=CYS6 PE=1 SV=2)

HSP 1 Score: 158.3 bits (399), Expect = 8.6e-38
Identity = 84/140 (60.00%), Postives = 106/140 (75.71%), Query Frame = 0

Query: 19  LLLLLFFSWIIVLTGNASAH--FCAEQDDPLLMASTLGGVHDSQGASNSTDVDELARFAV 78
           LL ++FFS  + ++   ++   FC E+       + +GGV D     NS +V+ LARFAV
Sbjct: 7   LLFIVFFSLSLFISSLIASDLGFCNEE------MALVGGVGDVPANQNSGEVESLARFAV 66

Query: 79  EEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLYEAKVWVKSWMNFKELQ 138
           +EHNKKEN+LLEF RVVKAKEQVVAGTLHHLT+E++EAG+KKLYEAKVWVK W+NFKELQ
Sbjct: 67  DEHNKKENALLEFARVVKAKEQVVAGTLHHLTLEILEAGQKKLYEAKVWVKPWLNFKELQ 126

Query: 139 EFKHAGDVPSISPSDLGAKK 157
           EFK A D P+I+ SDLG K+
Sbjct: 127 EFKPASDAPAITSSDLGCKQ 140

BLAST of IVF0022875 vs. ExPASy Swiss-Prot
Match: Q0JNR2 (Cysteine proteinase inhibitor 12 OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0270100 PE=2 SV=1)

HSP 1 Score: 156.4 bits (394), Expect = 3.3e-37
Identity = 81/146 (55.48%), Postives = 106/146 (72.60%), Query Frame = 0

Query: 11  PLSSTLSLLLLLLFFSWIIVLTGNASAHFCAEQDDPLLMASTLGGVHDSQGASNSTDVDE 70
           P SS+ +  L L     ++ +   A+A F        +    LGG HD+  A+NS + D 
Sbjct: 9   PASSSAAAPLPLFL---LLAVAAAAAALFLVGSASLAMAGHVLGGAHDAPSAANSVETDA 68

Query: 71  LARFAVEEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLYEAKVWVKSWM 130
           LARFAV+EHNK+EN+LLEFVRVV+AKEQVVAGTLHHLT+E +EAG+KK+YEAKVWVK W+
Sbjct: 69  LARFAVDEHNKRENALLEFVRVVEAKEQVVAGTLHHLTLEALEAGRKKVYEAKVWVKPWL 128

Query: 131 NFKELQEFKHAGDVPSISPSDLGAKK 157
           +FKELQEF++ GD  + + +DLGAKK
Sbjct: 129 DFKELQEFRNTGDATTFTNADLGAKK 151

BLAST of IVF0022875 vs. ExPASy Swiss-Prot
Match: Q06445 (Cysteine proteinase inhibitor OS=Vigna unguiculata OX=3917 PE=1 SV=1)

HSP 1 Score: 129.0 bits (323), Expect = 5.6e-29
Identity = 61/96 (63.54%), Postives = 77/96 (80.21%), Query Frame = 0

Query: 51  STLGGVHDSQGASNSTDVDELARFAVEEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVE 110
           + LGG  D  G  NS ++D LARFAVEEHNKK+N+LLEF RVV A++QVV+GTL+ +T+E
Sbjct: 2   AALGGNRDVAGNQNSLEIDSLARFAVEEHNKKQNALLEFGRVVSAQQQVVSGTLYTITLE 61

Query: 111 VVEAGKKKLYEAKVWVKSWMNFKELQEFKHAGDVPS 147
             + G+KK+YEAKVW K W+NFKELQEFKH GD P+
Sbjct: 62  AKDGGQKKVYEAKVWEKPWLNFKELQEFKHVGDAPA 97

BLAST of IVF0022875 vs. ExPASy Swiss-Prot
Match: Q41906 (Cysteine proteinase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=CYS3 PE=2 SV=2)

HSP 1 Score: 127.1 bits (318), Expect = 2.1e-28
Identity = 60/117 (51.28%), Postives = 83/117 (70.94%), Query Frame = 0

Query: 27  WII--VLTGNASAHFCAEQDDPLLMASTLGGVHDSQGASNSTDVDELARFAVEEHNKKEN 86
           WI+  +L G      C  ++        LGGVHD +G  NS +++ LARFA++EHNK++N
Sbjct: 7   WIVTLLLCGTIQLAICRSEEKSTEKTMMLGGVHDLRGNQNSGEIESLARFAIQEHNKQQN 66

Query: 87  SLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLYEAKVWVKSWMNFKELQEFKHA 142
            +LEF ++VKA+EQVVAGT++HLT+E  E  + K +EAKVWVK WMNFK+LQEFK +
Sbjct: 67  KILEFKKIVKAREQVVAGTMYHLTLEAKEGDQTKNFEAKVWVKPWMNFKQLQEFKES 123

BLAST of IVF0022875 vs. ExPASy Swiss-Prot
Match: Q8LC76 (Cysteine proteinase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=CYS7 PE=2 SV=2)

HSP 1 Score: 125.6 bits (314), Expect = 6.2e-28
Identity = 70/143 (48.95%), Postives = 103/143 (72.03%), Query Frame = 0

Query: 17  SLLLLLLFFSWIIVLTGNASAHFCAEQD---DPLLMASTLGGVHDSQGASN-STDVDELA 76
           S+ ++L+  S +++L+G      C+E+    +  ++   LGG  DS+   N   ++D++A
Sbjct: 7   SMCMMLICVS-LVLLSGFGQFVICSEEKGTYNDNVVKMKLGGFSDSKNDWNGGKEIDDIA 66

Query: 77  RFAVEEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLYEAKVWVKSWMNF 136
            FAV+EHN++EN++LE  RV+KA EQVVAG L+ LT+EV+EAG+KK+YEAKVWVK WMNF
Sbjct: 67  LFAVQEHNRRENAVLELARVLKATEQVVAGKLYRLTLEVIEAGEKKIYEAKVWVKPWMNF 126

Query: 137 KELQEFKHAGDVPSISPSDLGAK 156
           K+LQEFK+   +PS + SDLG K
Sbjct: 127 KQLQEFKNI--IPSFTISDLGFK 146

BLAST of IVF0022875 vs. ExPASy TrEMBL
Match: A0A5A7SXY4 (Cysteine proteinase inhibitor OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G001180 PE=3 SV=1)

HSP 1 Score: 303.5 bits (776), Expect = 6.1e-79
Identity = 156/156 (100.00%), Postives = 156/156 (100.00%), Query Frame = 0

Query: 1   MNNHSTISFSPLSSTLSLLLLLLFFSWIIVLTGNASAHFCAEQDDPLLMASTLGGVHDSQ 60
           MNNHSTISFSPLSSTLSLLLLLLFFSWIIVLTGNASAHFCAEQDDPLLMASTLGGVHDSQ
Sbjct: 1   MNNHSTISFSPLSSTLSLLLLLLFFSWIIVLTGNASAHFCAEQDDPLLMASTLGGVHDSQ 60

Query: 61  GASNSTDVDELARFAVEEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLY 120
           GASNSTDVDELARFAVEEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLY
Sbjct: 61  GASNSTDVDELARFAVEEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLY 120

Query: 121 EAKVWVKSWMNFKELQEFKHAGDVPSISPSDLGAKK 157
           EAKVWVKSWMNFKELQEFKHAGDVPSISPSDLGAKK
Sbjct: 121 EAKVWVKSWMNFKELQEFKHAGDVPSISPSDLGAKK 156

BLAST of IVF0022875 vs. ExPASy TrEMBL
Match: A0A5D3CW86 (Cysteine proteinase inhibitor OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G002960 PE=3 SV=1)

HSP 1 Score: 301.6 bits (771), Expect = 2.3e-78
Identity = 155/156 (99.36%), Postives = 155/156 (99.36%), Query Frame = 0

Query: 1   MNNHSTISFSPLSSTLSLLLLLLFFSWIIVLTGNASAHFCAEQDDPLLMASTLGGVHDSQ 60
           MNNHSTISFSPLSSTLSLLLLLLFFSWIIVL GNASAHFCAEQDDPLLMASTLGGVHDSQ
Sbjct: 1   MNNHSTISFSPLSSTLSLLLLLLFFSWIIVLAGNASAHFCAEQDDPLLMASTLGGVHDSQ 60

Query: 61  GASNSTDVDELARFAVEEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLY 120
           GASNSTDVDELARFAVEEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLY
Sbjct: 61  GASNSTDVDELARFAVEEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLY 120

Query: 121 EAKVWVKSWMNFKELQEFKHAGDVPSISPSDLGAKK 157
           EAKVWVKSWMNFKELQEFKHAGDVPSISPSDLGAKK
Sbjct: 121 EAKVWVKSWMNFKELQEFKHAGDVPSISPSDLGAKK 156

BLAST of IVF0022875 vs. ExPASy TrEMBL
Match: A0A1S3BEK2 (Cysteine proteinase inhibitor OS=Cucumis melo OX=3656 GN=LOC103488820 PE=3 SV=1)

HSP 1 Score: 301.6 bits (771), Expect = 2.3e-78
Identity = 155/156 (99.36%), Postives = 155/156 (99.36%), Query Frame = 0

Query: 1   MNNHSTISFSPLSSTLSLLLLLLFFSWIIVLTGNASAHFCAEQDDPLLMASTLGGVHDSQ 60
           MNNHSTISFSPLSSTLSLLLLLLFFSWIIVL GNASAHFCAEQDDPLLMASTLGGVHDSQ
Sbjct: 1   MNNHSTISFSPLSSTLSLLLLLLFFSWIIVLAGNASAHFCAEQDDPLLMASTLGGVHDSQ 60

Query: 61  GASNSTDVDELARFAVEEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLY 120
           GASNSTDVDELARFAVEEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLY
Sbjct: 61  GASNSTDVDELARFAVEEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLY 120

Query: 121 EAKVWVKSWMNFKELQEFKHAGDVPSISPSDLGAKK 157
           EAKVWVKSWMNFKELQEFKHAGDVPSISPSDLGAKK
Sbjct: 121 EAKVWVKSWMNFKELQEFKHAGDVPSISPSDLGAKK 156

BLAST of IVF0022875 vs. ExPASy TrEMBL
Match: A0A0A0KV53 (Cysteine proteinase inhibitor OS=Cucumis sativus OX=3659 GN=Csa_5G583360 PE=3 SV=1)

HSP 1 Score: 270.0 bits (689), Expect = 7.5e-69
Identity = 143/156 (91.67%), Postives = 147/156 (94.23%), Query Frame = 0

Query: 1   MNNHSTISFSPLSSTLSLLLLLLFFSWIIVLTGNASAHFCAEQDDPLLMASTLGGVHDSQ 60
           MN  ++ISFSPLSS L LLLLLLFFS IIVL GNASAHFCA+QDDPLLMASTLG V DSQ
Sbjct: 1   MNKDTSISFSPLSSAL-LLLLLLFFSGIIVLAGNASAHFCADQDDPLLMASTLGDVRDSQ 60

Query: 61  GASNSTDVDELARFAVEEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLY 120
           GASNS DVDELARFAVEEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLY
Sbjct: 61  GASNSADVDELARFAVEEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLY 120

Query: 121 EAKVWVKSWMNFKELQEFKHAGDVPSISPSDLGAKK 157
           EAKVWVKSWMNFKELQEFKHAGDVPSI+PSDLGAKK
Sbjct: 121 EAKVWVKSWMNFKELQEFKHAGDVPSITPSDLGAKK 155

BLAST of IVF0022875 vs. ExPASy TrEMBL
Match: A0A6J1J8G5 (Cysteine proteinase inhibitor OS=Cucurbita maxima OX=3661 GN=LOC111484419 PE=3 SV=1)

HSP 1 Score: 250.4 bits (638), Expect = 6.1e-63
Identity = 141/192 (73.44%), Postives = 155/192 (80.73%), Query Frame = 0

Query: 1   MNNHSTISFSPLSSTLSLLLLLLFFSWIIVLTGNASAHFCAEQDDPLLMASTLGGVHDSQ 60
           M N S+I FS  SST  LLLLL   SW IVL GNASAHFCA+QDDPLLMASTLGGVHDSQ
Sbjct: 1   MKNDSSIWFS--SSTFLLLLLL---SW-IVLAGNASAHFCAQQDDPLLMASTLGGVHDSQ 60

Query: 61  GASNSTDVDELARFAVEEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLY 120
           GASNS DVDELARFAV+EHNKKEN+LLEFVRVVKAKEQVVAGTLHHLTVEV +AGKKKLY
Sbjct: 61  GASNSADVDELARFAVDEHNKKENALLEFVRVVKAKEQVVAGTLHHLTVEVADAGKKKLY 120

Query: 121 EAKVWVKSWMNFKELQEFKHAGDVPSISPSDLGAKKVIIPRD--GVKCHHMILMFRMQHS 180
           EAK+WVKSWMNFKELQEFKHAGD PS SPSDLGAKK   P+    V  H   +    QH+
Sbjct: 121 EAKIWVKSWMNFKELQEFKHAGDAPSFSPSDLGAKKGDHPQGWREVPLHDPHVQDAAQHA 180

Query: 181 M-LFERSSRDLI 190
           + + ++ S  L+
Sbjct: 181 LRIIQQRSNSLL 186

BLAST of IVF0022875 vs. NCBI nr
Match: KAA0034127.1 (cysteine proteinase inhibitor 12 [Cucumis melo var. makuwa])

HSP 1 Score: 303 bits (777), Expect = 8.70e-102
Identity = 156/156 (100.00%), Postives = 156/156 (100.00%), Query Frame = 0

Query: 1   MNNHSTISFSPLSSTLSLLLLLLFFSWIIVLTGNASAHFCAEQDDPLLMASTLGGVHDSQ 60
           MNNHSTISFSPLSSTLSLLLLLLFFSWIIVLTGNASAHFCAEQDDPLLMASTLGGVHDSQ
Sbjct: 1   MNNHSTISFSPLSSTLSLLLLLLFFSWIIVLTGNASAHFCAEQDDPLLMASTLGGVHDSQ 60

Query: 61  GASNSTDVDELARFAVEEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLY 120
           GASNSTDVDELARFAVEEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLY
Sbjct: 61  GASNSTDVDELARFAVEEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLY 120

Query: 121 EAKVWVKSWMNFKELQEFKHAGDVPSISPSDLGAKK 156
           EAKVWVKSWMNFKELQEFKHAGDVPSISPSDLGAKK
Sbjct: 121 EAKVWVKSWMNFKELQEFKHAGDVPSISPSDLGAKK 156

BLAST of IVF0022875 vs. NCBI nr
Match: XP_008445944.1 (PREDICTED: cysteine proteinase inhibitor 12 [Cucumis melo] >TYK15792.1 cysteine proteinase inhibitor 12 [Cucumis melo var. makuwa])

HSP 1 Score: 301 bits (772), Expect = 5.02e-101
Identity = 155/156 (99.36%), Postives = 155/156 (99.36%), Query Frame = 0

Query: 1   MNNHSTISFSPLSSTLSLLLLLLFFSWIIVLTGNASAHFCAEQDDPLLMASTLGGVHDSQ 60
           MNNHSTISFSPLSSTLSLLLLLLFFSWIIVL GNASAHFCAEQDDPLLMASTLGGVHDSQ
Sbjct: 1   MNNHSTISFSPLSSTLSLLLLLLFFSWIIVLAGNASAHFCAEQDDPLLMASTLGGVHDSQ 60

Query: 61  GASNSTDVDELARFAVEEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLY 120
           GASNSTDVDELARFAVEEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLY
Sbjct: 61  GASNSTDVDELARFAVEEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLY 120

Query: 121 EAKVWVKSWMNFKELQEFKHAGDVPSISPSDLGAKK 156
           EAKVWVKSWMNFKELQEFKHAGDVPSISPSDLGAKK
Sbjct: 121 EAKVWVKSWMNFKELQEFKHAGDVPSISPSDLGAKK 156

BLAST of IVF0022875 vs. NCBI nr
Match: XP_004147084.1 (cysteine proteinase inhibitor 12 [Cucumis sativus] >KGN51606.1 hypothetical protein Csa_008462 [Cucumis sativus])

HSP 1 Score: 270 bits (690), Expect = 1.43e-88
Identity = 143/156 (91.67%), Postives = 147/156 (94.23%), Query Frame = 0

Query: 1   MNNHSTISFSPLSSTLSLLLLLLFFSWIIVLTGNASAHFCAEQDDPLLMASTLGGVHDSQ 60
           MN  ++ISFSPLSS L LLLLLLFFS IIVL GNASAHFCA+QDDPLLMASTLG V DSQ
Sbjct: 1   MNKDTSISFSPLSSAL-LLLLLLFFSGIIVLAGNASAHFCADQDDPLLMASTLGDVRDSQ 60

Query: 61  GASNSTDVDELARFAVEEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLY 120
           GASNS DVDELARFAVEEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLY
Sbjct: 61  GASNSADVDELARFAVEEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLY 120

Query: 121 EAKVWVKSWMNFKELQEFKHAGDVPSISPSDLGAKK 156
           EAKVWVKSWMNFKELQEFKHAGDVPSI+PSDLGAKK
Sbjct: 121 EAKVWVKSWMNFKELQEFKHAGDVPSITPSDLGAKK 155

BLAST of IVF0022875 vs. NCBI nr
Match: XP_038891963.1 (cysteine proteinase inhibitor 12 [Benincasa hispida])

HSP 1 Score: 257 bits (657), Expect = 1.34e-83
Identity = 136/156 (87.18%), Postives = 147/156 (94.23%), Query Frame = 0

Query: 1   MNNHSTISFSPLSSTLSLLLLLLFFSWIIVLTGNASAHFCAEQDDPLLMASTLGGVHDSQ 60
           MN +S+IS   LSS+++LLLLLLF SWIIVL GNASA FCA+QDDPLLMASTLGGV DSQ
Sbjct: 1   MNKNSSIS---LSSSIALLLLLLF-SWIIVLAGNASAQFCAQQDDPLLMASTLGGVRDSQ 60

Query: 61  GASNSTDVDELARFAVEEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLY 120
           GASNSTDVDELARFAV+EHNKKENS+LEFVRVVKAKEQVVAGTLHHLT+EVV+AGKKKLY
Sbjct: 61  GASNSTDVDELARFAVDEHNKKENSILEFVRVVKAKEQVVAGTLHHLTLEVVDAGKKKLY 120

Query: 121 EAKVWVKSWMNFKELQEFKHAGDVPSISPSDLGAKK 156
           EAKVWVKSWMNFKELQEFKHAGDVPSI+ SDLGAKK
Sbjct: 121 EAKVWVKSWMNFKELQEFKHAGDVPSITSSDLGAKK 152

BLAST of IVF0022875 vs. NCBI nr
Match: XP_023548422.1 (cysteine proteinase inhibitor 12-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 251 bits (642), Expect = 2.39e-81
Identity = 135/156 (86.54%), Postives = 141/156 (90.38%), Query Frame = 0

Query: 1   MNNHSTISFSPLSSTLSLLLLLLFFSWIIVLTGNASAHFCAEQDDPLLMASTLGGVHDSQ 60
           M N S+I FS  SSTL LLLLL   SWI VL GNASAHFCA+QD+PLLMASTLGGVHDSQ
Sbjct: 1   MKNDSSIWFS--SSTLLLLLLL---SWI-VLAGNASAHFCAQQDNPLLMASTLGGVHDSQ 60

Query: 61  GASNSTDVDELARFAVEEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLY 120
           GASNS DVDELARFAV+EHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEV +AGKKKLY
Sbjct: 61  GASNSADVDELARFAVDEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEVADAGKKKLY 120

Query: 121 EAKVWVKSWMNFKELQEFKHAGDVPSISPSDLGAKK 156
           EAK+WVKSWMNFKELQEFKHAGD PS SPSDLGAKK
Sbjct: 121 EAKIWVKSWMNFKELQEFKHAGDAPSFSPSDLGAKK 150

BLAST of IVF0022875 vs. TAIR 10
Match: AT3G12490.2 (cystatin B )

HSP 1 Score: 158.3 bits (399), Expect = 6.1e-39
Identity = 84/140 (60.00%), Postives = 106/140 (75.71%), Query Frame = 0

Query: 19  LLLLLFFSWIIVLTGNASAH--FCAEQDDPLLMASTLGGVHDSQGASNSTDVDELARFAV 78
           LL ++FFS  + ++   ++   FC E+       + +GGV D     NS +V+ LARFAV
Sbjct: 7   LLFIVFFSLSLFISSLIASDLGFCNEE------MALVGGVGDVPANQNSGEVESLARFAV 66

Query: 79  EEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLYEAKVWVKSWMNFKELQ 138
           +EHNKKEN+LLEF RVVKAKEQVVAGTLHHLT+E++EAG+KKLYEAKVWVK W+NFKELQ
Sbjct: 67  DEHNKKENALLEFARVVKAKEQVVAGTLHHLTLEILEAGQKKLYEAKVWVKPWLNFKELQ 126

Query: 139 EFKHAGDVPSISPSDLGAKK 157
           EFK A D P+I+ SDLG K+
Sbjct: 127 EFKPASDAPAITSSDLGCKQ 140

BLAST of IVF0022875 vs. TAIR 10
Match: AT3G12490.1 (cystatin B )

HSP 1 Score: 155.6 bits (392), Expect = 4.0e-38
Identity = 76/104 (73.08%), Postives = 89/104 (85.58%), Query Frame = 0

Query: 53  LGGVHDSQGASNSTDVDELARFAVEEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEVV 112
           +GGV D     NS +V+ LARFAV+EHNKKEN+LLEF RVVKAKEQVVAGTLHHLT+E++
Sbjct: 4   VGGVGDVPANQNSGEVESLARFAVDEHNKKENALLEFARVVKAKEQVVAGTLHHLTLEIL 63

Query: 113 EAGKKKLYEAKVWVKSWMNFKELQEFKHAGDVPSISPSDLGAKK 157
           EAG+KKLYEAKVWVK W+NFKELQEFK A D P+I+ SDLG K+
Sbjct: 64  EAGQKKLYEAKVWVKPWLNFKELQEFKPASDAPAITSSDLGCKQ 107

BLAST of IVF0022875 vs. TAIR 10
Match: AT2G40880.1 (cystatin A )

HSP 1 Score: 127.1 bits (318), Expect = 1.5e-29
Identity = 60/117 (51.28%), Postives = 83/117 (70.94%), Query Frame = 0

Query: 27  WII--VLTGNASAHFCAEQDDPLLMASTLGGVHDSQGASNSTDVDELARFAVEEHNKKEN 86
           WI+  +L G      C  ++        LGGVHD +G  NS +++ LARFA++EHNK++N
Sbjct: 7   WIVTLLLCGTIQLAICRSEEKSTEKTMMLGGVHDLRGNQNSGEIESLARFAIQEHNKQQN 66

Query: 87  SLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLYEAKVWVKSWMNFKELQEFKHA 142
            +LEF ++VKA+EQVVAGT++HLT+E  E  + K +EAKVWVK WMNFK+LQEFK +
Sbjct: 67  KILEFKKIVKAREQVVAGTMYHLTLEAKEGDQTKNFEAKVWVKPWMNFKQLQEFKES 123

BLAST of IVF0022875 vs. TAIR 10
Match: AT5G05110.1 (Cystatin/monellin family protein )

HSP 1 Score: 125.6 bits (314), Expect = 4.4e-29
Identity = 70/143 (48.95%), Postives = 103/143 (72.03%), Query Frame = 0

Query: 17  SLLLLLLFFSWIIVLTGNASAHFCAEQD---DPLLMASTLGGVHDSQGASN-STDVDELA 76
           S+ ++L+  S +++L+G      C+E+    +  ++   LGG  DS+   N   ++D++A
Sbjct: 7   SMCMMLICVS-LVLLSGFGQFVICSEEKGTYNDNVVKMKLGGFSDSKNDWNGGKEIDDIA 66

Query: 77  RFAVEEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLYEAKVWVKSWMNF 136
            FAV+EHN++EN++LE  RV+KA EQVVAG L+ LT+EV+EAG+KK+YEAKVWVK WMNF
Sbjct: 67  LFAVQEHNRRENAVLELARVLKATEQVVAGKLYRLTLEVIEAGEKKIYEAKVWVKPWMNF 126

Query: 137 KELQEFKHAGDVPSISPSDLGAK 156
           K+LQEFK+   +PS + SDLG K
Sbjct: 127 KQLQEFKNI--IPSFTISDLGFK 146

BLAST of IVF0022875 vs. TAIR 10
Match: AT5G12140.1 (cystatin-1 )

HSP 1 Score: 102.4 bits (254), Expect = 4.0e-22
Identity = 51/92 (55.43%), Postives = 64/92 (69.57%), Query Frame = 0

Query: 53  LGGVHDSQGASNSTDVDELARFAVEEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEVV 112
           +GGV D    +N   V+ LARFAV+EHNK EN  LE+ R++ AK QVVAGT+HHLTVEV 
Sbjct: 10  VGGVRDIDANANDLQVESLARFAVDEHNKNENLTLEYKRLLGAKTQVVAGTMHHLTVEVA 69

Query: 113 EAGKKKLYEAKVWVKSWMNFKELQEFKHAGDV 145
           +    K+YEAKV  K+W N K+L+ F H  DV
Sbjct: 70  DGETNKVYEAKVLEKAWENLKQLESFNHLHDV 101

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8H0X68.6e-3860.00Cysteine proteinase inhibitor 6 OS=Arabidopsis thaliana OX=3702 GN=CYS6 PE=1 SV=... [more]
Q0JNR23.3e-3755.48Cysteine proteinase inhibitor 12 OS=Oryza sativa subsp. japonica OX=39947 GN=Os0... [more]
Q064455.6e-2963.54Cysteine proteinase inhibitor OS=Vigna unguiculata OX=3917 PE=1 SV=1[more]
Q419062.1e-2851.28Cysteine proteinase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=CYS3 PE=2 SV=... [more]
Q8LC766.2e-2848.95Cysteine proteinase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=CYS7 PE=2 SV=... [more]
Match NameE-valueIdentityDescription
A0A5A7SXY46.1e-79100.00Cysteine proteinase inhibitor OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_sc... [more]
A0A5D3CW862.3e-7899.36Cysteine proteinase inhibitor OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc... [more]
A0A1S3BEK22.3e-7899.36Cysteine proteinase inhibitor OS=Cucumis melo OX=3656 GN=LOC103488820 PE=3 SV=1[more]
A0A0A0KV537.5e-6991.67Cysteine proteinase inhibitor OS=Cucumis sativus OX=3659 GN=Csa_5G583360 PE=3 SV... [more]
A0A6J1J8G56.1e-6373.44Cysteine proteinase inhibitor OS=Cucurbita maxima OX=3661 GN=LOC111484419 PE=3 S... [more]
Match NameE-valueIdentityDescription
KAA0034127.18.70e-102100.00cysteine proteinase inhibitor 12 [Cucumis melo var. makuwa][more]
XP_008445944.15.02e-10199.36PREDICTED: cysteine proteinase inhibitor 12 [Cucumis melo] >TYK15792.1 cysteine ... [more]
XP_004147084.11.43e-8891.67cysteine proteinase inhibitor 12 [Cucumis sativus] >KGN51606.1 hypothetical prot... [more]
XP_038891963.11.34e-8387.18cysteine proteinase inhibitor 12 [Benincasa hispida][more]
XP_023548422.12.39e-8186.54cysteine proteinase inhibitor 12-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT3G12490.26.1e-3960.00cystatin B [more]
AT3G12490.14.0e-3873.08cystatin B [more]
AT2G40880.11.5e-2951.28cystatin A [more]
AT5G05110.14.4e-2948.95Cystatin/monellin family protein [more]
AT5G12140.14.0e-2255.43cystatin-1 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000010Cystatin domainSMARTSM00043CY_4coord: 51..141
e-value: 3.8E-33
score: 126.1
IPR000010Cystatin domainPFAMPF16845SQAPIcoord: 65..140
e-value: 7.9E-21
score: 74.2
IPR000010Cystatin domainCDDcd00042CYcoord: 54..140
e-value: 3.5389E-25
score: 92.1519
NoneNo IPR availableGENE3D3.10.450.10coord: 49..148
e-value: 3.3E-42
score: 145.0
NoneNo IPR availablePANTHERPTHR11413:SF110CYSTEINE PROTEINASE INHIBITOR 7coord: 11..155
NoneNo IPR availableSUPERFAMILY54403Cystatin/monellincoord: 52..144
IPR027214CystatinPANTHERPTHR11413CYSTATIN FAMILY MEMBERcoord: 11..155
IPR018073Proteinase inhibitor I25, cystatin, conserved sitePROSITEPS00287CYSTATINcoord: 97..110

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
IVF0022875.1IVF0022875.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010951 negative regulation of endopeptidase activity
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004869 cysteine-type endopeptidase inhibitor activity