IVF0022541 (gene) Melon (IVF77) v1

BLAST of IVF0022541 vs. ExPASy Swiss-Prot
Match: Q8R149 (BUD13 homolog OS=Mus musculus OX=10090 GN=Bud13 PE=1 SV=1)

HSP 1 Score: 113.6 bits (283), Expect = 4.9e-24
Identity = 118/363 (32.51%), Postives = 165/363 (45.45%), Query Frame = 0

Query: 32  EEGEDFSPPRRRPRKP--PSSLEHDEKPTNSTYPNIDLSPPRQRRKRYHTPSPEPEATRT 91
           E   D SPPR+R  K    +  + D K       + DLSPPR+++   H  S        
Sbjct: 338 EHDSDLSPPRKRQAKAHFEAKKQLDSKGVYQKASDSDLSPPRKKKNSGHQDS-------- 397

Query: 92  RSVSPQSDMSPPRRSDRKASKASLGGNHKVDLSPPRRRASDYVGDEHISRGSDLSPPRKQ 151
                 SD+SPPR   R+ S  S       DLSPPRRR      D      SDLSPPR+ 
Sbjct: 398 -----DSDLSPPRNRPRRQSSDS-------DLSPPRRRQRTKSSD------SDLSPPRRS 457

Query: 152 RKDVRGDRSLLDKHSQNHVTDASQESRTDLSPHRKSKK--------NYQFQFKEMNPSAS 211
            +  +    +        VTD  +E       H++ KK          QF+F E      
Sbjct: 458 PRPGKKTAHMYSGAKTGLVTDVQRE-------HQELKKQDQDTTDLGAQFEFTE------ 517

Query: 212 SNADPVYRDKIKGDRISKEEFLKARGKIEEKPK--EIKLEWGKGLAQKREAEAELMELEL 271
                V+RDK    R  K E L+ R K E+  +  E+  +WGKGLAQ R+ +  + +   
Sbjct: 518 ----TVFRDKSGRKRNLKLERLEQRRKAEKDSERDELYAQWGKGLAQSRQQQQNVEDAMK 577

Query: 272 EKDRPFARSRNDAELDSMLRDRLRWGDPMAHLVKKRQSEMALPDLGDNEKMKESGFMIPQ 331
           E  +P AR  +D +LD MLR++ R GDPMA+ +KK +++        N+K+K        
Sbjct: 578 EMQKPLARYIDDEDLDRMLREQEREGDPMANFIKKNKAKE-----NKNKKVK-------- 637

Query: 332 DIPPHSWLKRGLDAAPNRYGIRPEDIGMGLIVATVRFEKQMFKRMNEKRATEREAYLWSV 383
             P +S    G    PNR+ I P     G +  +  FE++ F R+  K+A E  AY WSV
Sbjct: 638 --PRYS----GPAPPPNRFNIWPGYRWDG-VDRSNGFEQKRFARLASKKAVEELAYKWSV 637

BLAST of IVF0022541 vs. ExPASy Swiss-Prot
Match: Q4QQU1 (BUD13 homolog OS=Rattus norvegicus OX=10116 GN=Bud13 PE=1 SV=1)

HSP 1 Score: 99.8 bits (247), Expect = 7.3e-20
Identity = 135/453 (29.80%), Postives = 192/453 (42.38%), Query Frame = 0

Query: 5   DMSPPRRTRVRNDTPSPSNELKPPEPGEEGEDFSPPRRRPRK----PPSSLEHDEKPTNS 64
           D SPPR  RVR+DTP PS    PP       D SPPRR  R      P    H    T+S
Sbjct: 210 DPSPPR--RVRHDTPDPS----PPRRVRHDSDASPPRRSHRNSSAVSPKRGHHGSSGTSS 269

Query: 65  TYPNIDLSPPRQRRKRYHTPSPEPEATRTRSVSP-------QSDMSPPRRSDRKASKASL 124
                + SP   + +R    S      R R  SP       +S  +  R S +  S++ L
Sbjct: 270 PRQAHNHSPAAAQHRRTLDSSGAQHHRRARHDSPDLELPKAKSSKAAERPSSKTVSQSGL 329

Query: 125 GG-----------NHKVDLSPPRRRAS----------DYVGDEHISRGSDLSPPRKQRK- 184
           G             H  DLSPPR+R +          D  G    +  SDLSPPRK  K 
Sbjct: 330 GPPHPSLSMNSKYEHDSDLSPPRKRQAKAHFGAKKQLDSKGVCQKASDSDLSPPRKNSKS 389

Query: 185 ---DVRGDRSLLDKHSQNHVTDA----------SQESRTDLSPHRKSKK----------- 244
              D   D S      +   +D+          ++ S +DLSP R+S +           
Sbjct: 390 GHQDSDSDLSPPRNRPRRQSSDSDLSPPRRRQRTKSSDSDLSPSRRSPRSGKKTPHMYSG 449

Query: 245 -------NYQFQFKEMNPSASSNAD---------PVYRDKIKGDRISKEEFLKARGKIEE 304
                  + Q + +E+        D          V+RDK    R  K E L+ R K E+
Sbjct: 450 AKTGLVADVQREHQELKKQDQDATDLGAQFEFTETVFRDKSGRKRNLKLERLEQRRKAEK 509

Query: 305 KPK--EIKLEWGKGLAQKREAEAELMELELEKDRPFARSRNDAELDSMLRDRLRWGDPMA 364
             +  E+  +WGKGLAQ R+ +  + +   E  +P AR  +D +LD MLR++ R GDPMA
Sbjct: 510 DSERDELYAQWGKGLAQSRQQQQNVEDAMKEMQKPLARYIDDEDLDRMLREQEREGDPMA 569

Query: 365 HLVKKRQSEMALPDLGDNEKMKESGFMIPQDIPPHSWLKRGLDAAPNRYGIRPEDIGMGL 383
           + +KK +++        N+K++          P ++    G    PNR+ I P     G 
Sbjct: 570 NFIKKNKAKE-----NKNKKVR----------PRYN----GPAPPPNRFNIWPGYRWDG- 629

BLAST of IVF0022541 vs. ExPASy Swiss-Prot
Match: Q9BRD0 (BUD13 homolog OS=Homo sapiens OX=9606 GN=BUD13 PE=1 SV=1)

HSP 1 Score: 94.4 bits (233), Expect = 3.1e-18
Identity = 125/428 (29.21%), Postives = 187/428 (43.69%), Query Frame = 0

Query: 3   NSDMSPPRRTRVRNDTPSPSNELKPPEPGE-------EGEDFSP------PRRRPRKPP- 62
           +SD+S PR  RV N++P  S         +       +  D +P      PR +  K P 
Sbjct: 235 SSDISSPR--RVHNNSPDTSRRTLGSSDTQQLRRARHDSPDLAPNVTYSLPRTKSGKAPE 294

Query: 63  ------------SSLEHDEKPTNSTYP-NIDLSPPRQRRKRYHTPSPE--------PEAT 122
                       S   H   P NS Y  + D+SPPR+++ + H    +         +AT
Sbjct: 295 RASSKTSPHWKESGASHLSFPKNSKYEYDPDISPPRKKQAKSHFGDKKQLDSKGDCQKAT 354

Query: 123 RTRSVSPQ-----------SDMSPPRRSDRKASKASLGGNHKVDLSPPRRRASDYVGDEH 182
            +   SP+           SD+SPPR   R  S  S       DLSPPRRR      D  
Sbjct: 355 DSDLSSPRHKQSPGHQDSDSDLSPPRNRPRHRSSDS-------DLSPPRRRQRTKSSD-- 414

Query: 183 ISRGSDLSPPRKQRKDVRGDRSLLDKHSQNHVTDASQESRTDLSPHRKSKKNYQFQFKEM 242
               SDLSPPR+ +   +    +        V    Q  + +L    +    ++ +F+  
Sbjct: 415 ----SDLSPPRRSQPPGKKAAHMYSGAKTGLVLTDIQREQQELKEQDQETMAFEAEFQ-- 474

Query: 243 NPSASSNADPVYRDKIKGDRISKEEFLKARGKIEEKPK--EIKLEWGKGLAQKREAEAEL 302
                  A+ V+RDK    R  K E L+ R K E+  +  E+  +WGKGLAQ R+ +  +
Sbjct: 475 ------YAETVFRDKSGRKRNLKLERLEQRRKAEKDSERDELYAQWGKGLAQSRQQQQNV 534

Query: 303 MELELEKDRPFARSRNDAELDSMLRDRLRWGDPMAHLVKKRQSEMALPDLGDNEKMKESG 362
            +   E  +P AR  +D +LD MLR++ R GDPMA+ +KK +++        N+K++   
Sbjct: 535 EDAMKEMQKPLARYIDDEDLDRMLREQEREGDPMANFIKKNKAKE-----NKNKKVR--- 594

Query: 363 FMIPQDIPPHSWLKRGLDAAPNRYGIRPEDIGMGLIVATVRFEKQMFKRMNEKRATEREA 383
                  P +S    G    PNR+ I P     G +  +  FE++ F R+  K+A E  A
Sbjct: 595 -------PRYS----GPAPPPNRFNIWPGYRWDG-VDRSNGFEQKRFARLASKKAVEELA 619

BLAST of IVF0022541 vs. ExPASy Swiss-Prot
Match: Q5ZIJ0 (BUD13 homolog OS=Gallus gallus OX=9031 GN=BUD13 PE=2 SV=1)

HSP 1 Score: 91.3 bits (225), Expect = 2.6e-17
Identity = 126/420 (30.00%), Postives = 182/420 (43.33%), Query Frame = 0

Query: 5   DMSPPRRTRVRNDTPSPSNELKPP-EPGEEGEDFSPPRRRPR-----KPPSSLEHD---- 64
           D+SPPRR R  +D+P    +L PP     +  D SPPRR+        PP    HD    
Sbjct: 178 DLSPPRRKR-HDDSP----DLSPPRRKRHDSPDLSPPRRKRHDSPDLSPPRRQRHDSPDP 237

Query: 65  EKPTNSTYPNIDLSPPR---------QRRKRYHT-PSPEP------------EATRTRSV 124
             P    + + DLSP           QR+KRY + P   P            + +RT+  
Sbjct: 238 SPPRKKRHDSPDLSPQEQQTTPDLSLQRKKRYDSDPDSSPSLRKRARSPGHKKPSRTKDA 297

Query: 125 SP------QSDMSPPRRSDRKASKASLGGNHKVDLSP--PRRRASDYVGDEHISRGSDLS 184
           SP       SD  PPRR  + AS+A        DLSP     RA      +H S+    S
Sbjct: 298 SPVKKLRRDSDSPPPRRGTQNASEA--------DLSPQGKNHRALPSGKGQHGSK----S 357

Query: 185 PPRKQRKDVRGDRSLLDKHSQNHVTDASQESRTDLSPHRKSKKNYQFQFKEMNPSASSNA 244
           PP   R     D+    K +      A   S   L   ++  +  +   K +    S + 
Sbjct: 358 PPDLSRHHYPDDKGSPKKGNMMSGVRAGLVSADVLRREQQELRKQERSSKHLE-EESRHT 417

Query: 245 DPVYRDKIKGDRISKEEFLKARGKIEEKPK--EIKLEWGKGLAQKREAEAELMELELEKD 304
           + ++RDK    R   +E L+ + K E K +  E    WG+GLAQ R+ +  + +   E  
Sbjct: 418 ETIFRDKSGRKRDLVQERLEQQQKDEAKSERDEQYARWGRGLAQGRQQQQNVEDAIKEMQ 477

Query: 305 RPFARSRNDAELDSMLRDRLRWGDPMAHLVKKRQSEMALPDLGDNEKMKESGFMIPQDIP 364
           +P AR  +D +LD MLR++ R GDPMA  ++KR+++ +       EK + SG       P
Sbjct: 478 KPLARYIDDEDLDRMLREQEREGDPMAEFIRKRKAKESKE---KKEKPRYSG-----PAP 537

Query: 365 PHSWLKRGLDAAPNRYGIRPEDIGMGLIVATVRFEKQMFKRMNEKRATEREAYLWSVSDM 383
           P            NR+ I P     G +  +  FE+Q F RM  K+A +  AY WS+ DM
Sbjct: 538 P-----------LNRFNIWPGHRWDG-VDRSNGFEQQFFARMANKKAVQELAYKWSIEDM 559

BLAST of IVF0022541 vs. ExPASy Swiss-Prot
Match: O94417 (Pre-mRNA-splicing factor cwf26 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=cwf26 PE=1 SV=2)

HSP 1 Score: 69.3 bits (168), Expect = 1.1e-10
Identity = 61/203 (30.05%), Postives = 96/203 (47.29%), Query Frame = 0

Query: 183 RKSKKNYQFQFKEMNPSASSNADPVYRDKIKGDRISKEEFLK-ARGKIEEKPKEIK--LE 242
           ++ +K+     +E+  S     + VYRD   G RI      K A+ K++EK +E +   E
Sbjct: 136 KREEKSSNLDEEELRKS----RETVYRD-ATGRRIDLVLARKEAKRKLKEKEEEARRQKE 195

Query: 243 WGKGLAQKREAEAELMELELEKDRPFARSRNDAELDSMLRDRLRWGDPMAHLVKKRQSEM 302
             +G+ Q R+ +  L ELE +K  P AR  +D E +  L++R RW DP A  +  +    
Sbjct: 196 QQQGVVQVRQQKEYLKELERQKTVPLARYEDDPEYNKELKERSRWNDPAASFLTNKPV-- 255

Query: 303 ALPDLGDNEKMKESGFMIPQDIPPHSWLKRGLDAAPNRYGIRPEDIGMGLIVATVRFEKQ 362
                  + K    G+                 A PNR+ IRP     G+I     FE +
Sbjct: 256 -------SSKATYQGY-----------------APPNRFNIRPGHRWDGIIRGN-GFENK 306

Query: 363 MFKRMNEKRATEREAYLWSVSDM 383
            F+R NE++A E EA++W++ DM
Sbjct: 316 WFQRQNERKAQEHEAHMWAIEDM 306

BLAST of IVF0022541 vs. ExPASy TrEMBL
Match: A0A1S3CHR8 (BUD13 homolog OS=Cucumis melo OX=3656 GN=LOC103501110 PE=3 SV=1)

HSP 1 Score: 666.0 bits (1717), Expect = 9.4e-188
Identity = 362/442 (81.90%), Postives = 367/442 (83.03%), Query Frame = 0

Query: 1   MMNSDMSPPRRTRVRNDTPSPSNELKPPEPGEEGEDFSPPRRRPRKPPSSLEHDEKPTNS 60
           MMNSDMSPPRRTRVRNDTPSPSNELKPPEPGEEGEDFSPPRRRPRKPPSSLEHDEKPTNS
Sbjct: 117 MMNSDMSPPRRTRVRNDTPSPSNELKPPEPGEEGEDFSPPRRRPRKPPSSLEHDEKPTNS 176

Query: 61  TYP-----------------------------NIDLSPPRQRRKRYHTPSPEPEATRTRS 120
           TYP                             +IDLSPPRQRRKRYHTPSPEPEATRTRS
Sbjct: 177 TYPSSSPPQKDKVYRDAHLQGANWGHMDCAQEDIDLSPPRQRRKRYHTPSPEPEATRTRS 236

Query: 121 VSPQSDMSPPRRSDRKASKASLGGNHKVDLSPPRRRASDYVGDEHISRGSDLSPPRKQRK 180
           VSPQSDMSPPRRSDRKASKASLGGNHKVDLSPPRRRASDYVGDEHISRGSDLSPPRKQRK
Sbjct: 237 VSPQSDMSPPRRSDRKASKASLGGNHKVDLSPPRRRASDYVGDEHISRGSDLSPPRKQRK 296

Query: 181 DVRGDRSLLDKHSQNHVTDASQESRTDLSPHRKSKKNYQF-------------------- 240
           DVRGDRSLLDKHSQNHVTDASQESRTDLSPHRK +K                        
Sbjct: 297 DVRGDRSLLDKHSQNHVTDASQESRTDLSPHRKKQKELPVSVSFKQTRKTGLLTQQEFGE 356

Query: 241 -----------QFKEMNPSASSNADPVYRDKIKGDRISKEEFLKARGKIEEKPKEIKLEW 300
                      +FKEMNPSASSNADPVYRDKIKGDRISKEEFLKARGKIEEKPKEIKLEW
Sbjct: 357 EMSKTNKEDWTRFKEMNPSASSNADPVYRDKIKGDRISKEEFLKARGKIEEKPKEIKLEW 416

Query: 301 GKGLAQKREAEAELMELELEKDRPFARSRNDAELDSMLRDRLRWGDPMAHLVKKRQSEMA 360
           GKGLAQKREAEAELMELELEKDRPFARSRNDAELDSMLRDRLRWGDPMAHLVKKRQSEMA
Sbjct: 417 GKGLAQKREAEAELMELELEKDRPFARSRNDAELDSMLRDRLRWGDPMAHLVKKRQSEMA 476

Query: 361 LPDLGDNEKMKESGFMIPQDIPPHSWLKRGLDAAPNRYGIRPEDIGMGLIVATVRFEKQM 383
           LPDLGDNEKMKESGFMIPQDIPPHSWLKRGLDAAPNRYGIRP     G +  +  FEKQM
Sbjct: 477 LPDLGDNEKMKESGFMIPQDIPPHSWLKRGLDAAPNRYGIRPGRHWDG-VDRSNGFEKQM 536

BLAST of IVF0022541 vs. ExPASy TrEMBL
Match: A0A5A7VKV1 (BUD13-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold602G00590 PE=3 SV=1)

HSP 1 Score: 666.0 bits (1717), Expect = 9.4e-188
Identity = 362/442 (81.90%), Postives = 367/442 (83.03%), Query Frame = 0

Query: 1   MMNSDMSPPRRTRVRNDTPSPSNELKPPEPGEEGEDFSPPRRRPRKPPSSLEHDEKPTNS 60
           MMNSDMSPPRRTRVRNDTPSPSNELKPPEPGEEGEDFSPPRRRPRKPPSSLEHDEKPTNS
Sbjct: 117 MMNSDMSPPRRTRVRNDTPSPSNELKPPEPGEEGEDFSPPRRRPRKPPSSLEHDEKPTNS 176

Query: 61  TYP-----------------------------NIDLSPPRQRRKRYHTPSPEPEATRTRS 120
           TYP                             +IDLSPPRQRRKRYHTPSPEPEATRTRS
Sbjct: 177 TYPSSSPPQKDKVYRDAHLQGANWGHMDCAQEDIDLSPPRQRRKRYHTPSPEPEATRTRS 236

Query: 121 VSPQSDMSPPRRSDRKASKASLGGNHKVDLSPPRRRASDYVGDEHISRGSDLSPPRKQRK 180
           VSPQSDMSPPRRSDRKASKASLGGNHKVDLSPPRRRASDYVGDEHISRGSDLSPPRKQRK
Sbjct: 237 VSPQSDMSPPRRSDRKASKASLGGNHKVDLSPPRRRASDYVGDEHISRGSDLSPPRKQRK 296

Query: 181 DVRGDRSLLDKHSQNHVTDASQESRTDLSPHRKSKKNYQF-------------------- 240
           DVRGDRSLLDKHSQNHVTDASQESRTDLSPHRK +K                        
Sbjct: 297 DVRGDRSLLDKHSQNHVTDASQESRTDLSPHRKKQKELPVSVSFKQTRKTGLLTQQEFGE 356

Query: 241 -----------QFKEMNPSASSNADPVYRDKIKGDRISKEEFLKARGKIEEKPKEIKLEW 300
                      +FKEMNPSASSNADPVYRDKIKGDRISKEEFLKARGKIEEKPKEIKLEW
Sbjct: 357 EMSKTNKEDWTRFKEMNPSASSNADPVYRDKIKGDRISKEEFLKARGKIEEKPKEIKLEW 416

Query: 301 GKGLAQKREAEAELMELELEKDRPFARSRNDAELDSMLRDRLRWGDPMAHLVKKRQSEMA 360
           GKGLAQKREAEAELMELELEKDRPFARSRNDAELDSMLRDRLRWGDPMAHLVKKRQSEMA
Sbjct: 417 GKGLAQKREAEAELMELELEKDRPFARSRNDAELDSMLRDRLRWGDPMAHLVKKRQSEMA 476

Query: 361 LPDLGDNEKMKESGFMIPQDIPPHSWLKRGLDAAPNRYGIRPEDIGMGLIVATVRFEKQM 383
           LPDLGDNEKMKESGFMIPQDIPPHSWLKRGLDAAPNRYGIRP     G +  +  FEKQM
Sbjct: 477 LPDLGDNEKMKESGFMIPQDIPPHSWLKRGLDAAPNRYGIRPGRHWDG-VDRSNGFEKQM 536

BLAST of IVF0022541 vs. ExPASy TrEMBL
Match: A0A0A0LTH5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G428990 PE=3 SV=1)

HSP 1 Score: 632.1 bits (1629), Expect = 1.5e-177
Identity = 347/442 (78.51%), Postives = 358/442 (81.00%), Query Frame = 0

Query: 1   MMNSDMSPPRRTRVRNDTPSPSNELKPPEPGEEGEDFSPPRRRPRKPPSSLEHDEKPTNS 60
           M+NSDMSPPRRTRVRNDTPSPSNELKPP PGEEGEDFSPPRRRPRKPPSSLEHDEKPTNS
Sbjct: 116 MVNSDMSPPRRTRVRNDTPSPSNELKPPVPGEEGEDFSPPRRRPRKPPSSLEHDEKPTNS 175

Query: 61  TYP-----------------------------NIDLSPPRQRRKRYHTPSPEPEATRTRS 120
           TYP                             +IDLSPPRQRRKRYHTPSPEP+AT TRS
Sbjct: 176 TYPSSSPTQKDKVYRDAHLQGANSWHIDYAQEDIDLSPPRQRRKRYHTPSPEPDATCTRS 235

Query: 121 VSPQSDMSPPRRSDRKASKASLGGNHKVDLSPPRRRASDYVGDEHISRGSDLSPPRKQRK 180
           VSPQ D+SPPRRSDRKASK SLGGNHKVDLSPPRRRASDYVGDE ISRGSDLSPPRKQRK
Sbjct: 236 VSPQFDISPPRRSDRKASKTSLGGNHKVDLSPPRRRASDYVGDERISRGSDLSPPRKQRK 295

Query: 181 DVRGDRSLLDKHSQNHVTDASQESRTDLSPHRKSKKNYQF-------------------- 240
           DVRGDRSLLDK S+NHVTDASQES TDLSP RK +K                        
Sbjct: 296 DVRGDRSLLDKQSRNHVTDASQESPTDLSPPRKKQKELPVSVSFKQTRKTGLLTQQEFGE 355

Query: 241 -----------QFKEMNPSASSNADPVYRDKIKGDRISKEEFLKARGKIEEKPKEIKLEW 300
                      +FKEMNPSASSNADPVYRDKIKGDRISKEEFLKARGKIEEK KEIKLEW
Sbjct: 356 EMSKTNKEDWTRFKEMNPSASSNADPVYRDKIKGDRISKEEFLKARGKIEEKAKEIKLEW 415

Query: 301 GKGLAQKREAEAELMELELEKDRPFARSRNDAELDSMLRDRLRWGDPMAHLVKKRQSEMA 360
           GKGLAQKREAEAELMELELEKDRPFARSRNDAELD+MLRDRLRWGDPMAHLVKKRQSEMA
Sbjct: 416 GKGLAQKREAEAELMELELEKDRPFARSRNDAELDTMLRDRLRWGDPMAHLVKKRQSEMA 475

Query: 361 LPDLGDNEKMKESGFMIPQDIPPHSWLKRGLDAAPNRYGIRPEDIGMGLIVATVRFEKQM 383
           LPDLGDNEKMKESGFMIPQDIPPHSWLKRGLDAAPNRYGIRP     G +  +  FEKQM
Sbjct: 476 LPDLGDNEKMKESGFMIPQDIPPHSWLKRGLDAAPNRYGIRPGRHWDG-VDRSNGFEKQM 535

BLAST of IVF0022541 vs. ExPASy TrEMBL
Match: A0A6J1J7S6 (BUD13 homolog OS=Cucurbita maxima OX=3661 GN=LOC111484239 PE=3 SV=1)

HSP 1 Score: 560.1 bits (1442), Expect = 7.3e-156
Identity = 317/436 (72.71%), Postives = 337/436 (77.29%), Query Frame = 0

Query: 1   MMNSDMSPPRRTRVRNDTPSPSNELKPPEPGEEGEDFSPPRRRPRKPPSSLEHDEKPTNS 60
           M+N D+SPPRRTR RNDTPSPSNELKP   GEEGEDFSPPRRR R+ PSSLEH+EKPTNS
Sbjct: 1   MVNGDLSPPRRTRARNDTPSPSNELKPSVSGEEGEDFSPPRRRQRQNPSSLEHEEKPTNS 60

Query: 61  TYP-------------------NIDLSPPRQRRKRYHTPSPEPEATRTRSVSPQSDMSPP 120
             P                   +IDLSPPRQRR+RYHTPSPE +    RSVSPQSD+SPP
Sbjct: 61  KSPRSDLYQPQKQNVYRDCAQKDIDLSPPRQRRRRYHTPSPESDVKPARSVSPQSDLSPP 120

Query: 121 RRSDRKASKASLGGNHKV----DLSPPRRRASDYVGDEHISRGSDLSPPRKQRKDVRGDR 180
           RRSDR+ASKASLGGNHK     DLSPPRRR S Y  D HIS GSDLSPPRKQRKDV  DR
Sbjct: 121 RRSDRQASKASLGGNHKAAGLSDLSPPRRRTSGYADDAHISHGSDLSPPRKQRKDVSRDR 180

Query: 181 SLLDKHSQNH-VTDASQESRTDLSPHRK-----------------------------SKK 240
           SL DKH Q+H VTD SQES  DLSPHRK                             SK 
Sbjct: 181 SLSDKHLQSHIVTDDSQESPADLSPHRKKQKALPVSVSFKQARKTGLLTQKEFGEEMSKT 240

Query: 241 NYQ--FQFKEMNPSASSNADPVYRDKIKGDRISKEEFLKARGKIEEKPKEIKLEWGKGLA 300
           N +   +FKEMNPSASSNA+PVYRDKIKGDRISKE FLKARGKIEEKPKEIKLEWGKGLA
Sbjct: 241 NKEDWTRFKEMNPSASSNAEPVYRDKIKGDRISKEGFLKARGKIEEKPKEIKLEWGKGLA 300

Query: 301 QKREAEAELMELELEKDRPFARSRNDAELDSMLRDRLRWGDPMAHLVKKRQSEMALPDLG 360
           QKREAEAE MELELEK+RPFARS NDAELDSMLR+RLRWGDPMAHLVKK+QSEMALPDLG
Sbjct: 301 QKREAEAERMELELEKNRPFARSSNDAELDSMLRERLRWGDPMAHLVKKKQSEMALPDLG 360

Query: 361 DNEKMKESGFMIPQDIPPHSWLKRGLDAAPNRYGIRPEDIGMGLIVATVRFEKQMFKRMN 382
           D+EKMKESGF+IPQDIPPHSWLKRGLDAAPNRYGIRP     G +  +  FEKQMFKRMN
Sbjct: 361 DSEKMKESGFIIPQDIPPHSWLKRGLDAAPNRYGIRPGRHWDG-VDRSNGFEKQMFKRMN 420

BLAST of IVF0022541 vs. ExPASy TrEMBL
Match: A0A6J1KX65 (BUD13 homolog OS=Cucurbita maxima OX=3661 GN=LOC111498375 PE=3 SV=1)

HSP 1 Score: 549.7 bits (1415), Expect = 9.8e-153
Identity = 310/448 (69.20%), Postives = 339/448 (75.67%), Query Frame = 0

Query: 1   MMNSDMSPPRRTRVRNDTPSPSNELKPPEPGEEGEDFSPPRRRPRKPPSSLEHDEKPTNS 60
           +++SD+SPPRRTR RNDTPSPS+ELKP  PGEE ED SPPRRR RK PSSLEHDEKPT+S
Sbjct: 116 VVSSDISPPRRTRARNDTPSPSHELKP--PGEEAEDISPPRRRQRKHPSSLEHDEKPTSS 175

Query: 61  TYP-------------------------------NIDLSPPRQRRKRYHTPSPEPEATRT 120
           TYP                               +IDLSPPRQRRKRYHTPSPEP+   T
Sbjct: 176 TYPSSDSSPPRKQNVYRGTHLQGADSGHLDHAQEDIDLSPPRQRRKRYHTPSPEPDGKPT 235

Query: 121 RSVSPQSDMSPPRRSDRKASKASLGGNHKV----DLSPPRRRASDYVGDEHISRGSDLSP 180
           ++ SPQSDMSPPRR DR+ASK++LG NHK     DLSPPRRR S+Y  D ++SRGS LSP
Sbjct: 236 QTFSPQSDMSPPRRVDRQASKSNLGVNHKAAGLSDLSPPRRRTSNYAEDAYVSRGSYLSP 295

Query: 181 PRKQRKDVRGDRSLLDKHSQNHVTDASQESRTDLSPHRKSKK------------------ 240
           PR  R DVRGDR L   +SQNHVTDASQES  DLSP RK +K                  
Sbjct: 296 PRNHRVDVRGDRLLTKNNSQNHVTDASQESPPDLSPQRKQQKVPPVSVSFKQPRKTGLLT 355

Query: 241 NYQF-------------QFKEMNPSASSNADPVYRDKIKGDRISKEEFLKARGKIEEKPK 300
             +F             +FKEMNPSASSNA+P+YRDKIKGDRISKEEFLK+RGKIEEKPK
Sbjct: 356 QQEFGEEVSKTNKEDWTRFKEMNPSASSNAEPIYRDKIKGDRISKEEFLKSRGKIEEKPK 415

Query: 301 EIKLEWGKGLAQKREAEAELMELELEKDRPFARSRNDAELDSMLRDRLRWGDPMAHLVKK 360
           EIKLEWGKGLAQKREAEAE MELELEKDRPFARSRNDAELDSMLR+RLRWGDPMAHLVKK
Sbjct: 416 EIKLEWGKGLAQKREAEAEQMELELEKDRPFARSRNDAELDSMLRERLRWGDPMAHLVKK 475

Query: 361 RQSEMALPDLGDNEKMKESGFMIPQDIPPHSWLKRGLDAAPNRYGIRPEDIGMGLIVATV 383
           RQSEMALPDLGD+EKMKESGF+IPQDIPPHSWLKRGLDAAPNRYGIRP     G +  + 
Sbjct: 476 RQSEMALPDLGDSEKMKESGFIIPQDIPPHSWLKRGLDAAPNRYGIRPGRHWDG-VDRSN 535

BLAST of IVF0022541 vs. NCBI nr
Match: XP_008462822.1 (PREDICTED: BUD13 homolog [Cucumis melo] >XP_008462824.1 PREDICTED: BUD13 homolog [Cucumis melo] >KAA0066385.1 BUD13-like protein [Cucumis melo var. makuwa] >TYK00899.1 BUD13-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 663 bits (1710), Expect = 1.06e-235
Identity = 362/442 (81.90%), Postives = 367/442 (83.03%), Query Frame = 0

Query: 1   MMNSDMSPPRRTRVRNDTPSPSNELKPPEPGEEGEDFSPPRRRPRKPPSSLEHDEKPTNS 60
           MMNSDMSPPRRTRVRNDTPSPSNELKPPEPGEEGEDFSPPRRRPRKPPSSLEHDEKPTNS
Sbjct: 117 MMNSDMSPPRRTRVRNDTPSPSNELKPPEPGEEGEDFSPPRRRPRKPPSSLEHDEKPTNS 176

Query: 61  TYPN-----------------------------IDLSPPRQRRKRYHTPSPEPEATRTRS 120
           TYP+                             IDLSPPRQRRKRYHTPSPEPEATRTRS
Sbjct: 177 TYPSSSPPQKDKVYRDAHLQGANWGHMDCAQEDIDLSPPRQRRKRYHTPSPEPEATRTRS 236

Query: 121 VSPQSDMSPPRRSDRKASKASLGGNHKVDLSPPRRRASDYVGDEHISRGSDLSPPRKQRK 180
           VSPQSDMSPPRRSDRKASKASLGGNHKVDLSPPRRRASDYVGDEHISRGSDLSPPRKQRK
Sbjct: 237 VSPQSDMSPPRRSDRKASKASLGGNHKVDLSPPRRRASDYVGDEHISRGSDLSPPRKQRK 296

Query: 181 DVRGDRSLLDKHSQNHVTDASQESRTDLSPHRKSKKNYQF-------------------- 240
           DVRGDRSLLDKHSQNHVTDASQESRTDLSPHRK +K                        
Sbjct: 297 DVRGDRSLLDKHSQNHVTDASQESRTDLSPHRKKQKELPVSVSFKQTRKTGLLTQQEFGE 356

Query: 241 -----------QFKEMNPSASSNADPVYRDKIKGDRISKEEFLKARGKIEEKPKEIKLEW 300
                      +FKEMNPSASSNADPVYRDKIKGDRISKEEFLKARGKIEEKPKEIKLEW
Sbjct: 357 EMSKTNKEDWTRFKEMNPSASSNADPVYRDKIKGDRISKEEFLKARGKIEEKPKEIKLEW 416

Query: 301 GKGLAQKREAEAELMELELEKDRPFARSRNDAELDSMLRDRLRWGDPMAHLVKKRQSEMA 360
           GKGLAQKREAEAELMELELEKDRPFARSRNDAELDSMLRDRLRWGDPMAHLVKKRQSEMA
Sbjct: 417 GKGLAQKREAEAELMELELEKDRPFARSRNDAELDSMLRDRLRWGDPMAHLVKKRQSEMA 476

Query: 361 LPDLGDNEKMKESGFMIPQDIPPHSWLKRGLDAAPNRYGIRPEDIGMGLIVATVRFEKQM 382
           LPDLGDNEKMKESGFMIPQDIPPHSWLKRGLDAAPNRYGIRP     G +  +  FEKQM
Sbjct: 477 LPDLGDNEKMKESGFMIPQDIPPHSWLKRGLDAAPNRYGIRPGRHWDG-VDRSNGFEKQM 536

BLAST of IVF0022541 vs. NCBI nr
Match: XP_011650042.1 (BUD13 homolog [Cucumis sativus] >XP_031737093.1 BUD13 homolog [Cucumis sativus] >XP_031737094.1 BUD13 homolog [Cucumis sativus] >XP_031737095.1 BUD13 homolog [Cucumis sativus] >KGN63326.1 hypothetical protein Csa_021811 [Cucumis sativus])

HSP 1 Score: 629 bits (1622), Expect = 2.44e-222
Identity = 347/442 (78.51%), Postives = 358/442 (81.00%), Query Frame = 0

Query: 1   MMNSDMSPPRRTRVRNDTPSPSNELKPPEPGEEGEDFSPPRRRPRKPPSSLEHDEKPTNS 60
           M+NSDMSPPRRTRVRNDTPSPSNELKPP PGEEGEDFSPPRRRPRKPPSSLEHDEKPTNS
Sbjct: 116 MVNSDMSPPRRTRVRNDTPSPSNELKPPVPGEEGEDFSPPRRRPRKPPSSLEHDEKPTNS 175

Query: 61  TYPN-----------------------------IDLSPPRQRRKRYHTPSPEPEATRTRS 120
           TYP+                             IDLSPPRQRRKRYHTPSPEP+AT TRS
Sbjct: 176 TYPSSSPTQKDKVYRDAHLQGANSWHIDYAQEDIDLSPPRQRRKRYHTPSPEPDATCTRS 235

Query: 121 VSPQSDMSPPRRSDRKASKASLGGNHKVDLSPPRRRASDYVGDEHISRGSDLSPPRKQRK 180
           VSPQ D+SPPRRSDRKASK SLGGNHKVDLSPPRRRASDYVGDE ISRGSDLSPPRKQRK
Sbjct: 236 VSPQFDISPPRRSDRKASKTSLGGNHKVDLSPPRRRASDYVGDERISRGSDLSPPRKQRK 295

Query: 181 DVRGDRSLLDKHSQNHVTDASQESRTDLSPHRKSKKNYQF-------------------- 240
           DVRGDRSLLDK S+NHVTDASQES TDLSP RK +K                        
Sbjct: 296 DVRGDRSLLDKQSRNHVTDASQESPTDLSPPRKKQKELPVSVSFKQTRKTGLLTQQEFGE 355

Query: 241 -----------QFKEMNPSASSNADPVYRDKIKGDRISKEEFLKARGKIEEKPKEIKLEW 300
                      +FKEMNPSASSNADPVYRDKIKGDRISKEEFLKARGKIEEK KEIKLEW
Sbjct: 356 EMSKTNKEDWTRFKEMNPSASSNADPVYRDKIKGDRISKEEFLKARGKIEEKAKEIKLEW 415

Query: 301 GKGLAQKREAEAELMELELEKDRPFARSRNDAELDSMLRDRLRWGDPMAHLVKKRQSEMA 360
           GKGLAQKREAEAELMELELEKDRPFARSRNDAELD+MLRDRLRWGDPMAHLVKKRQSEMA
Sbjct: 416 GKGLAQKREAEAELMELELEKDRPFARSRNDAELDTMLRDRLRWGDPMAHLVKKRQSEMA 475

Query: 361 LPDLGDNEKMKESGFMIPQDIPPHSWLKRGLDAAPNRYGIRPEDIGMGLIVATVRFEKQM 382
           LPDLGDNEKMKESGFMIPQDIPPHSWLKRGLDAAPNRYGIRP     G +  +  FEKQM
Sbjct: 476 LPDLGDNEKMKESGFMIPQDIPPHSWLKRGLDAAPNRYGIRPGRHWDG-VDRSNGFEKQM 535

BLAST of IVF0022541 vs. NCBI nr
Match: XP_038901025.1 (BUD13 homolog [Benincasa hispida] >XP_038901026.1 BUD13 homolog [Benincasa hispida] >XP_038901027.1 BUD13 homolog [Benincasa hispida] >XP_038901028.1 BUD13 homolog [Benincasa hispida])

HSP 1 Score: 577 bits (1487), Expect = 9.71e-202
Identity = 327/447 (73.15%), Postives = 343/447 (76.73%), Query Frame = 0

Query: 1   MMNSDMSPPRRTRVRNDTPSPSNELKPPEPGEEGEDFSPPRRRPRKPPSSLEHDEKPTNS 60
           M+NSDMSPPRRTRVRNDTPSPSNELKPP  GEEGEDFS PRRR R+ PSSLEHDE PTNS
Sbjct: 117 MVNSDMSPPRRTRVRNDTPSPSNELKPPVSGEEGEDFSSPRRRQRQHPSSLEHDENPTNS 176

Query: 61  TYP------------------------------NIDLSPPRQRRKRYHTPSPEPEATRTR 120
            YP                              +IDLSPPRQRRKRYHTPSPEP+ T T 
Sbjct: 177 IYPPSSSPPQKQNVYRDTHLQGAIPEHMDCAQEDIDLSPPRQRRKRYHTPSPEPDVTHTH 236

Query: 121 SVSPQSDMSPPRRSDRKASKASLGGNHKV----DLSPPRRRASDYVGDEHISRGSDLSPP 180
           SVSPQSDMSPPRRSDR+ASKASLGGNHK     DLS PRRR SDY  D +ISRG DLSPP
Sbjct: 237 SVSPQSDMSPPRRSDRQASKASLGGNHKAAGLSDLSLPRRRTSDYADDAYISRGPDLSPP 296

Query: 181 RKQRKDVRGDRSLLDKHSQNHVTDASQESRTDLSPHRKSKK------------------N 240
           RKQRKDVRGDRSL DK S+NHVTDAS E   DLSP RK +K                   
Sbjct: 297 RKQRKDVRGDRSLSDKPSRNHVTDASPELLADLSPRRKQQKALPVSVSFKQARKTGLLTQ 356

Query: 241 YQF-------------QFKEMNPSASSNADPVYRDKIKGDRISKEEFLKARGKIEEKPKE 300
            +F             +FKEMNPSAS NA+PVYRDKIKGDRISKEEFLKARGK EEKPKE
Sbjct: 357 QEFGEEMSKTNKEDWTRFKEMNPSASGNAEPVYRDKIKGDRISKEEFLKARGKKEEKPKE 416

Query: 301 IKLEWGKGLAQKREAEAELMELELEKDRPFARSRNDAELDSMLRDRLRWGDPMAHLVKKR 360
           IKLEWGKGLAQKREAEAELMELELEKDRPFARSRNDAELDSMLRDRLRWGDPMAHLVKKR
Sbjct: 417 IKLEWGKGLAQKREAEAELMELELEKDRPFARSRNDAELDSMLRDRLRWGDPMAHLVKKR 476

Query: 361 QSEMALPDLGDNEKMKESGFMIPQDIPPHSWLKRGLDAAPNRYGIRPEDIGMGLIVATVR 382
           QSEMALPDLGD+EKMKESGF+IPQ+IPPHSWLKRGLDAAPNRYGIRP     G +  +  
Sbjct: 477 QSEMALPDLGDSEKMKESGFIIPQEIPPHSWLKRGLDAAPNRYGIRPGRHWDG-VDRSNG 536

BLAST of IVF0022541 vs. NCBI nr
Match: XP_022986527.1 (BUD13 homolog [Cucurbita maxima])

HSP 1 Score: 557 bits (1435), Expect = 1.70e-195
Identity = 316/436 (72.48%), Postives = 337/436 (77.29%), Query Frame = 0

Query: 1   MMNSDMSPPRRTRVRNDTPSPSNELKPPEPGEEGEDFSPPRRRPRKPPSSLEHDEKPTNS 60
           M+N D+SPPRRTR RNDTPSPSNELKP   GEEGEDFSPPRRR R+ PSSLEH+EKPTNS
Sbjct: 1   MVNGDLSPPRRTRARNDTPSPSNELKPSVSGEEGEDFSPPRRRQRQNPSSLEHEEKPTNS 60

Query: 61  TYP-------------------NIDLSPPRQRRKRYHTPSPEPEATRTRSVSPQSDMSPP 120
             P                   +IDLSPPRQRR+RYHTPSPE +    RSVSPQSD+SPP
Sbjct: 61  KSPRSDLYQPQKQNVYRDCAQKDIDLSPPRQRRRRYHTPSPESDVKPARSVSPQSDLSPP 120

Query: 121 RRSDRKASKASLGGNHKV----DLSPPRRRASDYVGDEHISRGSDLSPPRKQRKDVRGDR 180
           RRSDR+ASKASLGGNHK     DLSPPRRR S Y  D HIS GSDLSPPRKQRKDV  DR
Sbjct: 121 RRSDRQASKASLGGNHKAAGLSDLSPPRRRTSGYADDAHISHGSDLSPPRKQRKDVSRDR 180

Query: 181 SLLDKHSQNH-VTDASQESRTDLSPHRKSKK----NYQFQ-------------------- 240
           SL DKH Q+H VTD SQES  DLSPHRK +K    +  F+                    
Sbjct: 181 SLSDKHLQSHIVTDDSQESPADLSPHRKKQKALPVSVSFKQARKTGLLTQKEFGEEMSKT 240

Query: 241 -------FKEMNPSASSNADPVYRDKIKGDRISKEEFLKARGKIEEKPKEIKLEWGKGLA 300
                  FKEMNPSASSNA+PVYRDKIKGDRISKE FLKARGKIEEKPKEIKLEWGKGLA
Sbjct: 241 NKEDWTRFKEMNPSASSNAEPVYRDKIKGDRISKEGFLKARGKIEEKPKEIKLEWGKGLA 300

Query: 301 QKREAEAELMELELEKDRPFARSRNDAELDSMLRDRLRWGDPMAHLVKKRQSEMALPDLG 360
           QKREAEAE MELELEK+RPFARS NDAELDSMLR+RLRWGDPMAHLVKK+QSEMALPDLG
Sbjct: 301 QKREAEAERMELELEKNRPFARSSNDAELDSMLRERLRWGDPMAHLVKKKQSEMALPDLG 360

Query: 361 DNEKMKESGFMIPQDIPPHSWLKRGLDAAPNRYGIRPEDIGMGLIVATVRFEKQMFKRMN 381
           D+EKMKESGF+IPQDIPPHSWLKRGLDAAPNRYGIRP     G +  +  FEKQMFKRMN
Sbjct: 361 DSEKMKESGFIIPQDIPPHSWLKRGLDAAPNRYGIRPGRHWDG-VDRSNGFEKQMFKRMN 420

BLAST of IVF0022541 vs. NCBI nr
Match: XP_023005354.1 (BUD13 homolog [Cucurbita maxima] >XP_023005355.1 BUD13 homolog [Cucurbita maxima])

HSP 1 Score: 546 bits (1408), Expect = 8.88e-190
Identity = 310/448 (69.20%), Postives = 339/448 (75.67%), Query Frame = 0

Query: 1   MMNSDMSPPRRTRVRNDTPSPSNELKPPEPGEEGEDFSPPRRRPRKPPSSLEHDEKPTNS 60
           +++SD+SPPRRTR RNDTPSPS+ELKPP  GEE ED SPPRRR RK PSSLEHDEKPT+S
Sbjct: 116 VVSSDISPPRRTRARNDTPSPSHELKPP--GEEAEDISPPRRRQRKHPSSLEHDEKPTSS 175

Query: 61  TYPN-------------------------------IDLSPPRQRRKRYHTPSPEPEATRT 120
           TYP+                               IDLSPPRQRRKRYHTPSPEP+   T
Sbjct: 176 TYPSSDSSPPRKQNVYRGTHLQGADSGHLDHAQEDIDLSPPRQRRKRYHTPSPEPDGKPT 235

Query: 121 RSVSPQSDMSPPRRSDRKASKASLGGNHKV----DLSPPRRRASDYVGDEHISRGSDLSP 180
           ++ SPQSDMSPPRR DR+ASK++LG NHK     DLSPPRRR S+Y  D ++SRGS LSP
Sbjct: 236 QTFSPQSDMSPPRRVDRQASKSNLGVNHKAAGLSDLSPPRRRTSNYAEDAYVSRGSYLSP 295

Query: 181 PRKQRKDVRGDRSLLDKHSQNHVTDASQESRTDLSPHRKSKK------------------ 240
           PR  R DVRGDR L   +SQNHVTDASQES  DLSP RK +K                  
Sbjct: 296 PRNHRVDVRGDRLLTKNNSQNHVTDASQESPPDLSPQRKQQKVPPVSVSFKQPRKTGLLT 355

Query: 241 NYQF-------------QFKEMNPSASSNADPVYRDKIKGDRISKEEFLKARGKIEEKPK 300
             +F             +FKEMNPSASSNA+P+YRDKIKGDRISKEEFLK+RGKIEEKPK
Sbjct: 356 QQEFGEEVSKTNKEDWTRFKEMNPSASSNAEPIYRDKIKGDRISKEEFLKSRGKIEEKPK 415

Query: 301 EIKLEWGKGLAQKREAEAELMELELEKDRPFARSRNDAELDSMLRDRLRWGDPMAHLVKK 360
           EIKLEWGKGLAQKREAEAE MELELEKDRPFARSRNDAELDSMLR+RLRWGDPMAHLVKK
Sbjct: 416 EIKLEWGKGLAQKREAEAEQMELELEKDRPFARSRNDAELDSMLRERLRWGDPMAHLVKK 475

Query: 361 RQSEMALPDLGDNEKMKESGFMIPQDIPPHSWLKRGLDAAPNRYGIRPEDIGMGLIVATV 382
           RQSEMALPDLGD+EKMKESGF+IPQDIPPHSWLKRGLDAAPNRYGIRP     G +  + 
Sbjct: 476 RQSEMALPDLGDSEKMKESGFIIPQDIPPHSWLKRGLDAAPNRYGIRPGRHWDG-VDRSN 535

BLAST of IVF0022541 vs. TAIR 10
Match: AT1G31870.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: cytosol, nucleus; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF2050, pre-mRNA-splicing factor (InterPro:IPR018609); Has 13303 Blast hits to 9618 proteins in 489 species: Archae - 8; Bacteria - 444; Metazoa - 7153; Fungi - 2294; Plants - 942; Viruses - 128; Other Eukaryotes - 2334 (source: NCBI BLink). )

HSP 1 Score: 300.8 bits (769), Expect = 1.5e-81
Identity = 194/408 (47.55%), Postives = 258/408 (63.24%), Query Frame = 0

Query: 2   MNSDMSPPRRTRVRNDTPSPSNELKPPEPGEEGEDFSPPRRRPRKPPSSLEHDEKPTNST 61
           ++SDMSPPR+ + RND+PSP      PE     ED SPPRRR    PS  E   K ++S 
Sbjct: 173 LDSDMSPPRKRKARNDSPSPE-----PEAKYLSEDLSPPRRRHVHSPSR-ESSRKRSDSV 232

Query: 62  YPNIDLSPPRQ----------------------RRKRYHTPSPEPEATRTRSVSPQSDMS 121
             + DLSPPR+                      RR+RYH+PSPEP    ++S    +D+S
Sbjct: 233 ELDDDLSPPRRKRDLHGSPVSDVKKKSNDLSPPRRRRYHSPSPEPARRSSKSFGSNADLS 292

Query: 122 PPRRSDRKASKASLGGNHKVDLSPPRRRASDYVGDEHISRGS--DLSPPRKQRKDVRGDR 181
           PP R+       ++  +   DLSP R+ A      E++SR S  D SPPR+ R+D    +
Sbjct: 293 PPGRN------INMKDSQDSDLSPQRKAADLRRSSENVSRSSNFDSSPPRRPRRDSSPPQ 352

Query: 182 SLLDKHSQNHVTDASQESRTDL-SPHRKSKKNYQFQFKEMNPS-ASSNADPVYRDKIKGD 241
              ++     +      S  D+ S +RK K++ + +FK M+      NA+ V+RDKI G 
Sbjct: 353 ISKEQRKTGLI------SGKDIGSEYRKKKEDEKLRFKNMDSELTGQNAEAVFRDKITGK 412

Query: 242 RISKEEFLKAR-GKIEEKPKEIKLEWGKGLAQKREAEAELMELELEKDRPFARSRNDAEL 301
           RISKEE+LK++  K+ EKPKEIKLEWGKGLAQKREAEA L ELELEKD+PFAR+R+D EL
Sbjct: 413 RISKEEYLKSKQKKVIEKPKEIKLEWGKGLAQKREAEARLQELELEKDKPFARTRDDPEL 472

Query: 302 DSMLRDRLRWGDPMAHLVKKRQSEMALPDLGDNEKMKESGFMIPQDIPPHSWLKRGLDAA 361
           D M+++R+R+GDPMAHLVKKR+ E  L DLGD+E+MK+SGF+IPQ +P HSWL R L+AA
Sbjct: 473 DQMMKERVRFGDPMAHLVKKRKYETTLVDLGDDEEMKKSGFIIPQSVPKHSWLTRRLEAA 532

Query: 362 PNRYGIRPEDIGMGLIVATVRFEKQMFKRMNEKRATEREAYLWSVSDM 383
            NRYGI+P     G +  +   EK + K+ NE++ATE EAYLWSV+DM
Sbjct: 533 SNRYGIKPGRHWDG-VDRSNGTEKDLIKKTNERKATEIEAYLWSVADM 561

BLAST of IVF0022541 vs. TAIR 10
Match: AT1G31870.2 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: cytosol, nucleus; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF2050, pre-mRNA-splicing factor (InterPro:IPR018609); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink). )

HSP 1 Score: 300.8 bits (769), Expect = 1.5e-81
Identity = 194/408 (47.55%), Postives = 258/408 (63.24%), Query Frame = 0

Query: 2   MNSDMSPPRRTRVRNDTPSPSNELKPPEPGEEGEDFSPPRRRPRKPPSSLEHDEKPTNST 61
           ++SDMSPPR+ + RND+PSP      PE     ED SPPRRR    PS  E   K ++S 
Sbjct: 173 LDSDMSPPRKRKARNDSPSPE-----PEAKYLSEDLSPPRRRHVHSPSR-ESSRKRSDSV 232

Query: 62  YPNIDLSPPRQ----------------------RRKRYHTPSPEPEATRTRSVSPQSDMS 121
             + DLSPPR+                      RR+RYH+PSPEP    ++S    +D+S
Sbjct: 233 ELDDDLSPPRRKRDLHGSPVSDVKKKSNDLSPPRRRRYHSPSPEPARRSSKSFGSNADLS 292

Query: 122 PPRRSDRKASKASLGGNHKVDLSPPRRRASDYVGDEHISRGS--DLSPPRKQRKDVRGDR 181
           PP R+       ++  +   DLSP R+ A      E++SR S  D SPPR+ R+D    +
Sbjct: 293 PPGRN------INMKDSQDSDLSPQRKAADLRRSSENVSRSSNFDSSPPRRPRRDSSPPQ 352

Query: 182 SLLDKHSQNHVTDASQESRTDL-SPHRKSKKNYQFQFKEMNPS-ASSNADPVYRDKIKGD 241
              ++     +      S  D+ S +RK K++ + +FK M+      NA+ V+RDKI G 
Sbjct: 353 ISKEQRKTGLI------SGKDIGSEYRKKKEDEKLRFKNMDSELTGQNAEAVFRDKITGK 412

Query: 242 RISKEEFLKAR-GKIEEKPKEIKLEWGKGLAQKREAEAELMELELEKDRPFARSRNDAEL 301
           RISKEE+LK++  K+ EKPKEIKLEWGKGLAQKREAEA L ELELEKD+PFAR+R+D EL
Sbjct: 413 RISKEEYLKSKQKKVIEKPKEIKLEWGKGLAQKREAEARLQELELEKDKPFARTRDDPEL 472

Query: 302 DSMLRDRLRWGDPMAHLVKKRQSEMALPDLGDNEKMKESGFMIPQDIPPHSWLKRGLDAA 361
           D M+++R+R+GDPMAHLVKKR+ E  L DLGD+E+MK+SGF+IPQ +P HSWL R L+AA
Sbjct: 473 DQMMKERVRFGDPMAHLVKKRKYETTLVDLGDDEEMKKSGFIIPQSVPKHSWLTRRLEAA 532

Query: 362 PNRYGIRPEDIGMGLIVATVRFEKQMFKRMNEKRATEREAYLWSVSDM 383
            NRYGI+P     G +  +   EK + K+ NE++ATE EAYLWSV+DM
Sbjct: 533 SNRYGIKPGRHWDG-VDRSNGTEKDLIKKTNERKATEIEAYLWSVADM 561