IVF0022540 (gene) Melon (IVF77) v1

BLAST of IVF0022540 vs. ExPASy Swiss-Prot
Match: Q9FHK4 (Protein ENHANCED DISEASE RESISTANCE 4 OS=Arabidopsis thaliana OX=3702 GN=EDR4 PE=1 SV=1)

HSP 1 Score: 94.0 bits (232), Expect = 1.1e-17
Identity = 92/291 (31.62%), Postives = 123/291 (42.27%), Query Frame = 0

Query: 750  YPGQHSF-------GMEH-FPENFPHSRQLLPSEHWHNQGAR----MPHIDNDYYS---- 809
            YP  HS        GM H F +   H+    P      QG+R    M      +YS    
Sbjct: 372  YPRAHSLQMPSYAGGMNHDFVDYMYHNN---PRARGQGQGSRISGEMGRNHGGWYSGQLH 431

Query: 810  -QYNSCASSPQHFLTQLSARG------IHMQSDHLNHRNHGRNH------LREKNHFTKH 869
              Y+S ++SPQ  + Q           +    DH  +R+ G +H      LRE+    K 
Sbjct: 432  NSYSSYSASPQRPMEQPEYHPRWRREIVSDVEDHQRNRHAGHHHELQTRRLRERQRVAKR 491

Query: 870  HLRPMAGGTPFVTCYYCLKLLQIPAEFLLVKRRKR-LKCGHCSKVLEFSLQSRTHIVPCV 929
            H+RP AGG PFV+CY C + LQ+P +FL+ KR+   L+CG C+ VL FSLQSR H+VP V
Sbjct: 492  HVRPTAGGAPFVSCYSCSENLQLPVDFLIFKRKHHLLRCGTCTTVLRFSLQSRNHLVPAV 551

Query: 930  QSVSEPPPYEAHEHDDYDLAMGKSGSREIGDSIVLPHSSHQDMEKEHSSQNKFAHIKKCY 989
                                                  +H      +S+    + I K  
Sbjct: 552  --------------------------------------THDINANRNSNSTSESPIDKA- 611

Query: 990  QSGDPSSPAYKADKLSSEV--GKFSTKSNSPLHQLMGYSSPSQVFKGLDAP 1009
                PS P    +KL S V   +      SPLH+LMGYS+ SQVFK    P
Sbjct: 612  ----PSKP----EKLRSSVQDEELPVARGSPLHRLMGYSTVSQVFKVSQRP 612

HSP 2 Score: 63.5 bits (153), Expect = 1.5e-08
Identity = 49/149 (32.89%), Postives = 72/149 (48.32%), Query Frame = 0

Query: 1   MATGLTTKIRLVKCPRCRRLLPELPDIPVYKCGGCDAILVAKSQRDS-SHNPELGSQNRD 60
           MA+    KIRLV+CP+C ++L E  D+PVY+CGGC AIL AK +  + S  P  G   R 
Sbjct: 1   MASQTGQKIRLVRCPKCLKILQEDEDVPVYQCGGCSAILQAKRRNIAPSSTPSAGETERA 60

Query: 61  SAQRREVDPPSEGNQASISNPEAVIPPHGESNLVLRNGRGTNESREWSSENLVHSALSVQ 120
            A   +   P   N +S S  + V+P     ++     +G N S E + + L    LS  
Sbjct: 61  QANEPQ-SVPETNNVSSSSGQDTVLPSSPGRSVDQEYEKGRNASMESTEKELDDLELS-- 120

Query: 121 QRNARSDDETNHENGELSDGDLLKAEEAS 149
             N    +E   +   L D +  + E+ S
Sbjct: 121 --NGDGTNEIQEQECSLGDSEKNEREDNS 144

BLAST of IVF0022540 vs. ExPASy TrEMBL
Match: A0A1S3C426 (uncharacterized protein LOC103496635 OS=Cucumis melo OX=3656 GN=LOC103496635 PE=4 SV=1)

HSP 1 Score: 2014.6 bits (5218), Expect = 0.0e+00
Identity = 1014/1028 (98.64%), Postives = 1015/1028 (98.74%), Query Frame = 0

Query: 1    MATGLTTKIRLVKCPRCRRLLPELPDIPVYKCGGCDAILVAKSQRDSSHNPELGSQNRDS 60
            MATGLTTKIRLVKCPRCRRLLPELPDIPVYKCGGCDAILVAKSQRDSSHNPELGSQNRDS
Sbjct: 1    MATGLTTKIRLVKCPRCRRLLPELPDIPVYKCGGCDAILVAKSQRDSSHNPELGSQNRDS 60

Query: 61   AQRREVDPPSEGNQASISNPEAVIPPHGESNLVLRNGRGTNESREWSSENLVHSALSVQQ 120
            AQRREVDPPSEGNQASISNPEAVIPPHGESNLVLRNGRGTNESREWSSENLVHSALSVQQ
Sbjct: 61   AQRREVDPPSEGNQASISNPEAVIPPHGESNLVLRNGRGTNESREWSSENLVHSALSVQQ 120

Query: 121  RNARSDDETNHENGELSDGDLLKAEEASISSSSHEAIIPSSGECVIDPDAEEDQDEFGGS 180
            RNARSDDETNHENGELSDGDLLKAEEASISSSSHEAIIPSSGECVIDPDAEEDQDEFGGS
Sbjct: 121  RNARSDDETNHENGELSDGDLLKAEEASISSSSHEAIIPSSGECVIDPDAEEDQDEFGGS 180

Query: 181  SSEQLVRRKLGERQTYARTNNDSHAVNDGPLGFPSRDRSGEEATYHDNGKLPCRDAPEQE 240
            SSEQLVRRKLGERQTYARTNNDSHAVNDGPLGFPSRDRSGEEATYHDNGKLPCRDAPEQE
Sbjct: 181  SSEQLVRRKLGERQTYARTNNDSHAVNDGPLGFPSRDRSGEEATYHDNGKLPCRDAPEQE 240

Query: 241  EYCISSPEGTIPSSEECFTDLNDVKGQKECGDISQEQLAHIKSGECLKNSGSYNESPACH 300
            EYCISSPEGTIPSSEECFTDLNDVKGQKECGDISQEQLAHIKSGECLKNSGSYNESPACH
Sbjct: 241  EYCISSPEGTIPSSEECFTDLNDVKGQKECGDISQEQLAHIKSGECLKNSGSYNESPACH 300

Query: 301  GERPSCSNQSYSRIEFASHGKANLSGRDPTKVMETSTSSHEVMTIPFSGGSVLDPNDEKD 360
            GERPSCSNQSYSRIEFASHGKANLSGRDPTKVMETSTSSHEVMTIPFSGGSVLDPNDEKD
Sbjct: 301  GERPSCSNQSYSRIEFASHGKANLSGRDPTKVMETSTSSHEVMTIPFSGGSVLDPNDEKD 360

Query: 361  QKEEGNHNEQLVQIPFTDSLACHGKQSGSSDQVSSANEVANHENDKPSSVDHSEDEIVDQ 420
            QKEEGNHNEQLVQIPFTDSLACHGKQSGSSDQVSSANEVANHENDKPSSVDHSEDEIVDQ
Sbjct: 361  QKEEGNHNEQLVQIPFTDSLACHGKQSGSSDQVSSANEVANHENDKPSSVDHSEDEIVDQ 420

Query: 421  ILLSGEESPKADSKEIHSDLESSSISNAHVVTSISPKILAPLAQRGEAPLKITSHDSDHQ 480
            ILLSGEESPKADSKEIHSDLESSSISNAHVVTSISPKILAPLAQRGEAPLKITSHDSDHQ
Sbjct: 421  ILLSGEESPKADSKEIHSDLESSSISNAHVVTSISPKILAPLAQRGEAPLKITSHDSDHQ 480

Query: 481  TPVENIACMEVNQCSELSGGLPGVARFLTTKRSFACDGSVSSYDGMDDQFSDHDKRSLQN 540
            TPVENIACMEVNQCSELSGGLPGVARFLTTKRSFACDGSVSSYDGMDDQFSDHDKRSLQN
Sbjct: 481  TPVENIACMEVNQCSELSGGLPGVARFLTTKRSFACDGSVSSYDGMDDQFSDHDKRSLQN 540

Query: 541  NHHASTFLTTVERRRREESLMNNNAVARDSEVPIEGRNYKEISSHEKHYGIEYHERNQND 600
            NHHASTFLTTVERRRREESLMNNNAVARDSEVPIEGRNYKEISSHEKHYGIEYHERNQND
Sbjct: 541  NHHASTFLTTVERRRREESLMNNNAVARDSEVPIEGRNYKEISSHEKHYGIEYHERNQND 600

Query: 601  VLQHRRQDIPMQSRSRLRREKYQSKLSLLGTNHLGGYESGSASSSVFDEPHDSRMHLSE- 660
            VLQHRRQDIPMQSRSRLRREKYQSKLSLLGTNHLGGYESGSASSSVFDEPHDSRMHLSE 
Sbjct: 601  VLQHRRQDIPMQSRSRLRREKYQSKLSLLGTNHLGGYESGSASSSVFDEPHDSRMHLSEN 660

Query: 661  -----------ILMVYELQDQLEKSCNLNGNASERLSMGSVQRDGWVPMHYNHQIPQEES 720
                       + MVYELQDQLEKSCNLNGNASERLSMGSVQRDGWVPMHYNHQIPQEES
Sbjct: 661  FVDHDEDKARLLRMVYELQDQLEKSCNLNGNASERLSMGSVQRDGWVPMHYNHQIPQEES 720

Query: 721  WHDSEYPSYYRRNGPQINYSGQHPLSRMTSAVKAVSGPQVNYPGQHSFGMEHFPENFPHS 780
            WHDSEYPSYYRRNGPQINYSGQHPLSRMTSAVKAVSGPQVNYPGQHSFGMEHFPENFPHS
Sbjct: 721  WHDSEYPSYYRRNGPQINYSGQHPLSRMTSAVKAVSGPQVNYPGQHSFGMEHFPENFPHS 780

Query: 781  RQLLPSEHWHNQGARMPHIDNDYYSQYNSCASSPQHFLTQLSARGIHMQSDHLNHRNHGR 840
            RQLLPSEHWHNQGARMPHIDNDYYSQYNSCASSPQHFLTQLSARGIHMQSDHLNHRNHGR
Sbjct: 781  RQLLPSEHWHNQGARMPHIDNDYYSQYNSCASSPQHFLTQLSARGIHMQSDHLNHRNHGR 840

Query: 841  NHLREKNHFTKHHLRPMAGGTPFVTCYYCLKLLQIPAEFLLVKRRKRLKCGHCSKVLEFS 900
            NHLREKNHFTKHHLRPMAGGTPFVTCYYCLKLLQIPAEFLLVKRRKRLKCGHCSKVLEFS
Sbjct: 841  NHLREKNHFTKHHLRPMAGGTPFVTCYYCLKLLQIPAEFLLVKRRKRLKCGHCSKVLEFS 900

Query: 901  LQSRTHIVPCVQSVSEPPPYEAHEHDDYDLAMGKSGSREIGDSIVLPHSSHQDMEKEHSS 960
            LQSRTHIVPCVQSVSEPPPYEAHEHDDYDLAMGKSGSREIGDSIVLPHSSHQDMEKEHSS
Sbjct: 901  LQSRTHIVPCVQSVSEPPPYEAHEHDDYDLAMGKSGSREIGDSIVLPHSSHQDMEKEHSS 960

Query: 961  QNKFAHIKKCYQSGDPSSPAYKADKLSSEVGKFSTKSNSPLHQLMGYSSPSQVFKGLDAP 1017
            QNKFAHIKKCYQSGDPSSPAYKADKLSSEVGKFSTKSNSPLHQLMGYSSPSQVFKGLDAP
Sbjct: 961  QNKFAHIKKCYQSGDPSSPAYKADKLSSEVGKFSTKSNSPLHQLMGYSSPSQVFKGLDAP 1020

BLAST of IVF0022540 vs. ExPASy TrEMBL
Match: A0A5A7UEI7 (DUF3133 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold409G002290 PE=4 SV=1)

HSP 1 Score: 2006.1 bits (5196), Expect = 0.0e+00
Identity = 1014/1039 (97.59%), Postives = 1015/1039 (97.69%), Query Frame = 0

Query: 1    MATGLTTKIRLVKCPRCRRLLPELPDIPVYKCGGCDAILV-----------AKSQRDSSH 60
            MATGLTTKIRLVKCPRCRRLLPELPDIPVYKCGGCDAILV           AKSQRDSSH
Sbjct: 1    MATGLTTKIRLVKCPRCRRLLPELPDIPVYKCGGCDAILVVLIGFILTLHSAKSQRDSSH 60

Query: 61   NPELGSQNRDSAQRREVDPPSEGNQASISNPEAVIPPHGESNLVLRNGRGTNESREWSSE 120
            NPELGSQNRDSAQRREVDPPSEGNQASISNPEAVIPPHGESNLVLRNGRGTNESREWSSE
Sbjct: 61   NPELGSQNRDSAQRREVDPPSEGNQASISNPEAVIPPHGESNLVLRNGRGTNESREWSSE 120

Query: 121  NLVHSALSVQQRNARSDDETNHENGELSDGDLLKAEEASISSSSHEAIIPSSGECVIDPD 180
            NLVHSALSVQQRNARSDDETNHENGELSDGDLLKAEEASISSSSHEAIIPSSGECVIDPD
Sbjct: 121  NLVHSALSVQQRNARSDDETNHENGELSDGDLLKAEEASISSSSHEAIIPSSGECVIDPD 180

Query: 181  AEEDQDEFGGSSSEQLVRRKLGERQTYARTNNDSHAVNDGPLGFPSRDRSGEEATYHDNG 240
            AEEDQDEFGGSSSEQLVRRKLGERQTYARTNNDSHAVNDGPLGFPSRDRSGEEATYHDNG
Sbjct: 181  AEEDQDEFGGSSSEQLVRRKLGERQTYARTNNDSHAVNDGPLGFPSRDRSGEEATYHDNG 240

Query: 241  KLPCRDAPEQEEYCISSPEGTIPSSEECFTDLNDVKGQKECGDISQEQLAHIKSGECLKN 300
            KLPCRDAPEQEEYCISSPEGTIPSSEECFTDLNDVKGQKECGDISQEQLAHIKSGECLKN
Sbjct: 241  KLPCRDAPEQEEYCISSPEGTIPSSEECFTDLNDVKGQKECGDISQEQLAHIKSGECLKN 300

Query: 301  SGSYNESPACHGERPSCSNQSYSRIEFASHGKANLSGRDPTKVMETSTSSHEVMTIPFSG 360
            SGSYNESPACHGERPSCSNQSYSRIEFASHGKANLSGRDPTKVMETSTSSHEVMTIPFSG
Sbjct: 301  SGSYNESPACHGERPSCSNQSYSRIEFASHGKANLSGRDPTKVMETSTSSHEVMTIPFSG 360

Query: 361  GSVLDPNDEKDQKEEGNHNEQLVQIPFTDSLACHGKQSGSSDQVSSANEVANHENDKPSS 420
            GSVLDPNDEKDQKEEGNHNEQLVQIPFTDSLACHGKQSGSSDQVSSANEVANHENDKPSS
Sbjct: 361  GSVLDPNDEKDQKEEGNHNEQLVQIPFTDSLACHGKQSGSSDQVSSANEVANHENDKPSS 420

Query: 421  VDHSEDEIVDQILLSGEESPKADSKEIHSDLESSSISNAHVVTSISPKILAPLAQRGEAP 480
            VDHSEDEIVDQILLSGEESPKADSKEIHSDLESSSISNAHVVTSISPKILAPLAQRGEAP
Sbjct: 421  VDHSEDEIVDQILLSGEESPKADSKEIHSDLESSSISNAHVVTSISPKILAPLAQRGEAP 480

Query: 481  LKITSHDSDHQTPVENIACMEVNQCSELSGGLPGVARFLTTKRSFACDGSVSSYDGMDDQ 540
            LKITSHDSDHQTPVENIACMEVNQCSELSGGLPGVARFLTTKRSFACDGSVSSYDGMDDQ
Sbjct: 481  LKITSHDSDHQTPVENIACMEVNQCSELSGGLPGVARFLTTKRSFACDGSVSSYDGMDDQ 540

Query: 541  FSDHDKRSLQNNHHASTFLTTVERRRREESLMNNNAVARDSEVPIEGRNYKEISSHEKHY 600
            FSDHDKRSLQNNHHASTFLTTVERRRREESLMNNNAVARDSEVPIEGRNYKEISSHEKHY
Sbjct: 541  FSDHDKRSLQNNHHASTFLTTVERRRREESLMNNNAVARDSEVPIEGRNYKEISSHEKHY 600

Query: 601  GIEYHERNQNDVLQHRRQDIPMQSRSRLRREKYQSKLSLLGTNHLGGYESGSASSSVFDE 660
            GIEYHERNQNDVLQHRRQDIPMQSRSRLRREKYQSKLSLLGTNHLGGYESGSASSSVFDE
Sbjct: 601  GIEYHERNQNDVLQHRRQDIPMQSRSRLRREKYQSKLSLLGTNHLGGYESGSASSSVFDE 660

Query: 661  PHDSRMHLSE------------ILMVYELQDQLEKSCNLNGNASERLSMGSVQRDGWVPM 720
            PHDSRMHLSE            + MVYELQDQLEKSCNLNGNASERLSMGSVQRDGWVPM
Sbjct: 661  PHDSRMHLSENFVDHDEDKARLLRMVYELQDQLEKSCNLNGNASERLSMGSVQRDGWVPM 720

Query: 721  HYNHQIPQEESWHDSEYPSYYRRNGPQINYSGQHPLSRMTSAVKAVSGPQVNYPGQHSFG 780
            HYNHQIPQEESWHDSEYPSYYRRNGPQINYSGQHPLSRMTSAVKAVSGPQVNYPGQHSFG
Sbjct: 721  HYNHQIPQEESWHDSEYPSYYRRNGPQINYSGQHPLSRMTSAVKAVSGPQVNYPGQHSFG 780

Query: 781  MEHFPENFPHSRQLLPSEHWHNQGARMPHIDNDYYSQYNSCASSPQHFLTQLSARGIHMQ 840
            MEHFPENFPHSRQLLPSEHWHNQGARMPHIDNDYYSQYNSCASSPQHFLTQLSARGIHMQ
Sbjct: 781  MEHFPENFPHSRQLLPSEHWHNQGARMPHIDNDYYSQYNSCASSPQHFLTQLSARGIHMQ 840

Query: 841  SDHLNHRNHGRNHLREKNHFTKHHLRPMAGGTPFVTCYYCLKLLQIPAEFLLVKRRKRLK 900
            SDHLNHRNHGRNHLREKNHFTKHHLRPMAGGTPFVTCYYCLKLLQIPAEFLLVKRRKRLK
Sbjct: 841  SDHLNHRNHGRNHLREKNHFTKHHLRPMAGGTPFVTCYYCLKLLQIPAEFLLVKRRKRLK 900

Query: 901  CGHCSKVLEFSLQSRTHIVPCVQSVSEPPPYEAHEHDDYDLAMGKSGSREIGDSIVLPHS 960
            CGHCSKVLEFSLQSRTHIVPCVQSVSEPPPYEAHEHDDYDLAMGKSGSREIGDSIVLPHS
Sbjct: 901  CGHCSKVLEFSLQSRTHIVPCVQSVSEPPPYEAHEHDDYDLAMGKSGSREIGDSIVLPHS 960

Query: 961  SHQDMEKEHSSQNKFAHIKKCYQSGDPSSPAYKADKLSSEVGKFSTKSNSPLHQLMGYSS 1017
            SHQDMEKEHSSQNKFAHIKKCYQSGDPSSPAYKADKLSSEVGKFSTKSNSPLHQLMGYSS
Sbjct: 961  SHQDMEKEHSSQNKFAHIKKCYQSGDPSSPAYKADKLSSEVGKFSTKSNSPLHQLMGYSS 1020

BLAST of IVF0022540 vs. ExPASy TrEMBL
Match: A0A0A0KAI3 (zinc_ribbon_12 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G014640 PE=4 SV=1)

HSP 1 Score: 1821.6 bits (4717), Expect = 0.0e+00
Identity = 931/1032 (90.21%), Postives = 971/1032 (94.09%), Query Frame = 0

Query: 1    MATGLTTKIRLVKCPRCRRLLPELPDIPVYKCGGCDAILVAKSQRDSSHNPELGSQNRDS 60
            MATGLT KIRLVKCPRCRRLLPELPDIPVYKCGGCDAILVAKSQRDSSHN ELGSQNRDS
Sbjct: 1    MATGLTAKIRLVKCPRCRRLLPELPDIPVYKCGGCDAILVAKSQRDSSHNTELGSQNRDS 60

Query: 61   AQRREVDPPSEGNQASISNPEAVIPPHGESNLVLRNGRGTNESREWSSENLVHSALSVQQ 120
            AQR EV PPSEGNQASISN EAVIP HGESNLVL N R TNESREWSSENLVHSALSVQQ
Sbjct: 61   AQRHEVHPPSEGNQASISNHEAVIPRHGESNLVLSNERDTNESREWSSENLVHSALSVQQ 120

Query: 121  RNARSDDETNHENGELSDGDLLKAEEASISSSSHEAIIPSSGECVIDPDAEEDQDEFGGS 180
            RNARSDDETNHENGELSDGDLLKA+EASISSSSHEAIIPSSGECVIDPDAEEDQDEFGGS
Sbjct: 121  RNARSDDETNHENGELSDGDLLKADEASISSSSHEAIIPSSGECVIDPDAEEDQDEFGGS 180

Query: 181  SSEQLVRRKLGERQTYARTNNDSHAVNDGPLGFPSRDRSGEEATYHDNGKLPCRDAPEQE 240
            SSEQLVRRKLGE QT+AR  NDSHAVNDGPLGFPSRD SGEEAT+HDN KLPCRDAPEQE
Sbjct: 181  SSEQLVRRKLGECQTHAR--NDSHAVNDGPLGFPSRDCSGEEATHHDNRKLPCRDAPEQE 240

Query: 241  EYCISSPEGTIPSSEECFTDLNDVKGQKECGDISQEQLAHIKSGECLKNSGSYNESPACH 300
            +YC+SSPEGTIPSSEECFTD NDVKGQKECGDISQEQLAHIKS ECLK+SGSYNESPACH
Sbjct: 241  DYCMSSPEGTIPSSEECFTDPNDVKGQKECGDISQEQLAHIKSTECLKDSGSYNESPACH 300

Query: 301  GERPSCSNQSYSRIEFASHGKANLSGRDPTKVMETSTSSHEVMTIPFSGGSVLDPNDEKD 360
            GE+PSCSNQSYSRIEF+SHGKANLSG+DPTKV ETSTSSHEVMTIPFSG SV+DPNDE+D
Sbjct: 301  GEQPSCSNQSYSRIEFSSHGKANLSGQDPTKVTETSTSSHEVMTIPFSGESVVDPNDERD 360

Query: 361  QK-EEGNHNEQLVQIPFTDSLACHGKQSGSSDQVSSANEVANHENDKPSSVDHSEDEIVD 420
            QK EEGNH+EQLVQIP TDSLACHG+QSG SDQVSSANE+A+ ENDKPSSVDHSEDEIVD
Sbjct: 361  QKEEEGNHSEQLVQIPLTDSLACHGEQSGYSDQVSSANEIASRENDKPSSVDHSEDEIVD 420

Query: 421  QILLSGEESPKA-DSKEIHSDLESSSISNAHVVTSISPKILAPLAQRGEAPLKITSHDSD 480
            QILLSGEESPK  DSKEI SDLESSSISNAHV TSISPKILAPLA+RGEAPLKITSHDSD
Sbjct: 421  QILLSGEESPKLDDSKEIRSDLESSSISNAHVGTSISPKILAPLAERGEAPLKITSHDSD 480

Query: 481  HQTPVENIACMEVNQCSELSGGLPGVARFLTTKRSFACDGSVSSYDGMDDQFSDHDKRSL 540
            HQ PVE+ ACMEVNQCS+LSG + G+A+ LTTK SFACDGSVSSYDGMDDQF DH KRSL
Sbjct: 481  HQIPVESFACMEVNQCSKLSGDISGMAKILTTKHSFACDGSVSSYDGMDDQFLDHHKRSL 540

Query: 541  QNNHHASTFLTTVERRRREESLMNNNAVARDSEVPIEGRNYKEISSHEKHYGIEYHERNQ 600
            QNNHHA+TFLT VERRRRE+SLMN+NAVARDSEVPIEGRN +E SSHEKHYGIEYHERNQ
Sbjct: 541  QNNHHAATFLTAVERRRREQSLMNSNAVARDSEVPIEGRNSRETSSHEKHYGIEYHERNQ 600

Query: 601  NDVLQHRRQDIPMQSRSRLRREKYQSKLSLLGTNHLGGYESGSASSSVFDEPHDSRMHLS 660
            NDVL+HRRQDIPMQSRSRLRREKYQSKLSL GTNHLGGYESGSASSSVFDEPHDSRMHLS
Sbjct: 601  NDVLEHRRQDIPMQSRSRLRREKYQSKLSLHGTNHLGGYESGSASSSVFDEPHDSRMHLS 660

Query: 661  E------------ILMVYELQDQLEKSCNLNGNASERLSMGSVQRDGWVPMHYNHQIPQE 720
            E            + MVYELQDQLEKSCNLNGNASER+SMGSVQRDGW PM+Y+HQIPQE
Sbjct: 661  ENFVDQDEDKARLLRMVYELQDQLEKSCNLNGNASERVSMGSVQRDGWAPMYYDHQIPQE 720

Query: 721  ESWHDSEYPSYYRRNGPQINYSGQHPLSRMTSAVKAVSGPQVNYPGQHSFGMEHFPENFP 780
            ESWHDSEYPS++RRNGPQINYSGQHPLSRMTSAVK VSGPQVNYPGQHSFGMEHFPENFP
Sbjct: 721  ESWHDSEYPSFHRRNGPQINYSGQHPLSRMTSAVKVVSGPQVNYPGQHSFGMEHFPENFP 780

Query: 781  HSRQLLPSEHWHNQGARMPHIDNDYYSQYNSCASSPQHFL-TQLSARGIHMQSDHLNHRN 840
            HSRQLLPSEHWHNQGARMPHIDNDYYSQY+SCASSPQHFL TQLSARGIHMQSDHLNHRN
Sbjct: 781  HSRQLLPSEHWHNQGARMPHIDNDYYSQYSSCASSPQHFLSTQLSARGIHMQSDHLNHRN 840

Query: 841  HGRNHLREKNHFTKHHLRPMAGGTPFVTCYYCLKLLQIPAEFLLVKRR-KRLKCGHCSKV 900
            HGRNHLREKNHF KHHLRPMAGG PFVTCYYCLKLLQIPAEFLLVKRR KRLKCGHCSKV
Sbjct: 841  HGRNHLREKNHFAKHHLRPMAGGAPFVTCYYCLKLLQIPAEFLLVKRRCKRLKCGHCSKV 900

Query: 901  LEFSLQSRTHIVPCVQSVSEPPPYEAHEHDDYDLAMGKSGSREIGDSIVLPHSSHQDMEK 960
            LEFSLQSRTHIVP VQSV+EPPPYEAHEHD+Y LAMGKSGSREIGD+IVLPHSSHQDMEK
Sbjct: 901  LEFSLQSRTHIVPYVQSVAEPPPYEAHEHDNYALAMGKSGSREIGDTIVLPHSSHQDMEK 960

Query: 961  EHSSQNKFAHIKKCYQSGDPSSPAYKADKLSSEVGKFSTKSNSPLHQLMGYSSPSQVFKG 1017
            EHSS+NKF HIK+ YQSGDPSSPAYKADKLSSEVGKFSTKSNSPLH+LMGYSSPSQVFKG
Sbjct: 961  EHSSRNKFEHIKRSYQSGDPSSPAYKADKLSSEVGKFSTKSNSPLHRLMGYSSPSQVFKG 1020

BLAST of IVF0022540 vs. ExPASy TrEMBL
Match: A0A6J1K0X7 (protein ENHANCED DISEASE RESISTANCE 4-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111489161 PE=4 SV=1)

HSP 1 Score: 1184.1 bits (3062), Expect = 0.0e+00
Identity = 677/1046 (64.72%), Postives = 761/1046 (72.75%), Query Frame = 0

Query: 1    MATGLTTKIRLVKCPRCRRLLPELPDIPVYKCGGCDAILVAKSQRDSSHNPELGSQNRDS 60
            MATGLT KIRLVKCPRC RLLPELPDIPVYKCGGCDAILVAKS+R+SSH+ ELGSQNRDS
Sbjct: 1    MATGLTAKIRLVKCPRCHRLLPELPDIPVYKCGGCDAILVAKSRRESSHDTELGSQNRDS 60

Query: 61   AQRREVDPPSEGNQASISNPEAVIPPHGESNLVLRNGRGTNESREWSSENLVHSALSVQQ 120
            AQRREV P S   Q SISN EA+IPPH ES   L N R  NES E S+E LVHS+LSV Q
Sbjct: 61   AQRREVGPLSVERQPSISNHEAIIPPHDESTSGLNNDRDANESGERSNEELVHSSLSV-Q 120

Query: 121  RNARSDDETNHENGELSDGDLLKAEEASISSSSHEAIIPSSGECVIDPDAEEDQDEFGGS 180
            RNAR+DD ++HENGELSDGDLLK E ASISSSS EAII SSGE V+DPD EE QD+ G S
Sbjct: 121  RNARNDDGSHHENGELSDGDLLKVEVASISSSSPEAIISSSGEYVVDPDVEEVQDKSGDS 180

Query: 181  SSEQLVRRKLGERQTYARTNNDSHAVNDGPLGFPSRDRSGEEATYHDNGKLPCRDAPEQE 240
              EQLV +KL ERQ+YA  NN+S A N  P GF ++    EE TYH+N            
Sbjct: 181  CREQLVHKKLDERQSYAGNNNESRAGNGKPSGFSNQVYYEEECTYHENS----------- 240

Query: 241  EYCISSPEGTIPSSEECFTDLNDVKGQKECGDISQEQLAHIKSGECLKNSGSYNESPACH 300
               ISS E TIPSS +CF D ND K +KE    + EQLAHIKS ECLKN+ S  ES ACH
Sbjct: 241  ---ISSHEATIPSSGKCFIDPNDAKDRKES---AGEQLAHIKSSECLKNARSNGESQACH 300

Query: 301  GERPSCSNQSYSRIEFASHGKANLSGRDPTKVMETSTSSHEVMTIPFSGGSVLDPNDEKD 360
             E+ SCSNQSYSRIEFASHG   LSG DPTK METSTSSHEV+T+PFSG SVLD ND+KD
Sbjct: 301  DEQLSCSNQSYSRIEFASHGNDELSGPDPTKAMETSTSSHEVITVPFSGESVLDQNDDKD 360

Query: 361  QK-EEGNHNEQLVQIPFTDSLACHGKQSGSSDQVSSANEVANHENDKPSSVDHSEDEIVD 420
            QK EE NH+EQLVQ+                    S NE  N ENDK SS D SEDE +D
Sbjct: 361  QKEEEDNHSEQLVQV--------------------SLNEGVNRENDKLSSRDPSEDERLD 420

Query: 421  QILLSGEESPKADS-KEIHSDLESSSIS-----------NAHVVTSISPKILAPLAQRGE 480
            +ILLSGE SP+AD  KEIHS+LE+S IS           +  V TSIS K L+PLA+R E
Sbjct: 421  EILLSGEGSPEADDRKEIHSNLETSGISIAVATSSIIYPDTKVGTSISSKNLSPLAERAE 480

Query: 481  APLKITSHDSDHQTPVENIACMEVNQCSELSGGLPGVARFLTTKRSFACDGSVSSYDGMD 540
            AP +  SHD DH  PVEN  CMEVN+CSE SG LPG+ + LTTK  FA       YDGMD
Sbjct: 481  APEETISHDFDHHIPVENFGCMEVNRCSEPSGALPGMVKSLTTKSYFA-------YDGMD 540

Query: 541  DQFSDHDKRSLQNNHHASTFLTTVERRRREESLMNNNAVARDSEVPIEGRNYKEISSHEK 600
            D+FSD  +RSL+N H A  FL+TVE  RR+ESLM NNAV RDS++PIE  + +EI  HEK
Sbjct: 541  DRFSDRHRRSLKNTHEAVNFLSTVEGPRRQESLMKNNAVVRDSDIPIEAGSSQEILPHEK 600

Query: 601  HYGIEYHERNQNDVLQHRRQDIPMQSRSRLRREKYQSKLSLLGTNHLGGYESGSASSSVF 660
            HY I Y ERNQND+LQHRR  I MQSRSRLRREKYQSK SLL +N  G  E+GSAS+S  
Sbjct: 601  HYDIAYLERNQNDMLQHRRHGISMQSRSRLRREKYQSKSSLLRSNLEGDRENGSASNSTI 660

Query: 661  DEPHDSRMHLSE------------ILMVYELQDQLEKSCNLNGNASERLSMGSVQRDGWV 720
            + PHDSRMH SE            + MVYELQDQLEKSCNLN N   R+S GSVQ+D W 
Sbjct: 661  NGPHDSRMHSSENFVDPDEEKVRLLRMVYELQDQLEKSCNLNTNTGGRVSTGSVQKDTWA 720

Query: 721  PMHYNHQIPQEESWHDSEYPSYYRRNGPQINYSGQHPLSRMTSAVKAVSGPQVNYPGQHS 780
            PM+ N QI QEESWH+SE+P Y+RR+GPQ NY GQH LSRMTSA KAVSGPQ N      
Sbjct: 721  PMYNNQQISQEESWHESEHPYYFRRSGPQTNYPGQHSLSRMTSAAKAVSGPQNN-----C 780

Query: 781  FGMEHFPENFPHSRQLLPSEHWHNQGARMPHIDNDYYSQYNSCASSPQHF-LTQLSARGI 840
            F ME FP+N P   Q   SE WHN+GARM H+D+DYYSQYNSCASSPQHF  TQL ARGI
Sbjct: 781  FSMEQFPDNIPPLMQRRSSEVWHNRGARMAHMDHDYYSQYNSCASSPQHFHCTQLPARGI 840

Query: 841  HMQSDHLNHRNHGRNHLREKNHFTKHHLRPMAGGTPFVTCYYCLKLLQIPAEFLLVKRR- 900
             MQSD  +H N   N+LREK H  KHHLRPMAGG PF+TCY+CLKLLQIPAEFLLVKR  
Sbjct: 841  QMQSDQSSHGNLEMNYLREKRHLAKHHLRPMAGGAPFITCYHCLKLLQIPAEFLLVKRPC 900

Query: 901  KRLKCGHCSKVLEFSLQSRTHIVPCVQSVSEPPPYEAHEHDDYDLAMGKSGSREIGDSIV 960
             RLKCGHCSKVLEFSL +RTHIVP +Q+V+EPPPYEA EH+DY L +G  GSREI DSIV
Sbjct: 901  NRLKCGHCSKVLEFSLWNRTHIVPYIQNVAEPPPYEAEEHNDYALTIGNRGSREIDDSIV 960

Query: 961  LPHSSHQDMEKEHSSQ---NKFAHIKKCYQSGDPSSPAYKADKLSSEVGKFSTKSNSPLH 1017
            LPHSSH+ MEKE SS+   NKF  +KK YQSGDP          SS+VGK ST+S SPLH
Sbjct: 961  LPHSSHRGMEKELSSKWSSNKFESLKKSYQSGDP----------SSKVGKLSTESVSPLH 986

BLAST of IVF0022540 vs. ExPASy TrEMBL
Match: A0A6J1BXK6 (uncharacterized protein LOC111006528 OS=Momordica charantia OX=3673 GN=LOC111006528 PE=4 SV=1)

HSP 1 Score: 1179.9 bits (3051), Expect = 0.0e+00
Identity = 676/1060 (63.77%), Postives = 777/1060 (73.30%), Query Frame = 0

Query: 1    MATGLTTKIRLVKCPRCRRLLPELPDIPVYKCGGCDAILVAKSQRDSSHNPELGSQNRDS 60
            MATGLT KIRLVKCPRCRRLLPELPDIPVYKCGGC A+L AK++RDSSH+ ELGSQNRDS
Sbjct: 1    MATGLTAKIRLVKCPRCRRLLPELPDIPVYKCGGCGAVLQAKNRRDSSHDTELGSQNRDS 60

Query: 61   AQRREVDPPSEGNQASISNPEAVIPPHGESNLVLRNGRGTNESREWSSENLVHSALSVQQ 120
            AQR EV+PPSE  + SIS+  A+IP  GESNL L N R  NES EW SE LVH+ LS Q 
Sbjct: 61   AQRHEVNPPSEDREPSISSHGAIIPLRGESNLELNNERDRNESGEWGSERLVHTTLSEQL 120

Query: 121  RNARSDDETNHENGELSDGDLLKAEEASISSSSHEAIIPSSGECVIDPDAEEDQDEFGGS 180
             NAR++ E++HENGELSDGDLLKAEEASISSSSHEAIIPSSGECVIDPD E+DQDE   S
Sbjct: 121  MNARNNGESHHENGELSDGDLLKAEEASISSSSHEAIIPSSGECVIDPDVEKDQDE---S 180

Query: 181  SSEQLVRRKLGERQTYARTNNDSHAVNDGPLGFPSRDRSGEEATYHDNGKL--PCRDAPE 240
             SEQLVRRKLGERQ YAR N+   A +DG  GFP++    EE +YH+NGKL  P RD   
Sbjct: 181  GSEQLVRRKLGERQKYARNNDKPRASDDGRSGFPNQVYYEEECSYHENGKLPIPSRDPSV 240

Query: 241  QEEYCISSPEGTIPSSEECFTDLNDVKGQK---ECGDISQEQLAHIKSGECLKNSGSYNE 300
             E   ISSPE T+ SS ECF D ND+K QK   E GD S EQLA I S ECL N+GS +E
Sbjct: 241  GEADSISSPEATMSSSGECFIDPNDIKVQKDQNESGDCSLEQLADINSSECLNNAGSNDE 300

Query: 301  SPACHGERPSCSNQSYSRIEFASHGKANLSGRDPTKVMETSTSSHEVMTIPFSGGSVLDP 360
            S A H E+P  SNQ YSRIEFASHG A LSG+ PT+  ETSTSSHEV+TIPFSG S++DP
Sbjct: 301  SRAFHDEQPGGSNQFYSRIEFASHGNAKLSGQGPTEEEETSTSSHEVITIPFSGESIVDP 360

Query: 361  NDEKDQKE--EGNHNEQLVQIPF------------TDSLACHGKQSGSSDQVSSANEVAN 420
            NDEKD+KE  E N +EQLVQI              + + ACH  QSG SD+    N VA 
Sbjct: 361  NDEKDRKEGDEYNRSEQLVQITSSKHQKNASNGDESPAPACHDGQSGFSDKAFFVNGVAT 420

Query: 421  HENDKPSSVDHSEDEIVDQILLSGEESPKADS-KEIHSDLESSSISN-----------AH 480
              N+K  S D SE   +D+ILL GEESP+AD  K+IHS+LE S ISN             
Sbjct: 421  CGNEKLPSGDSSES--IDEILLLGEESPEADDRKKIHSNLECSGISNEVAKSSIITADTQ 480

Query: 481  VVTSISPKILAPLAQRGEAPLKITSHDSDHQTPVENIACMEVNQCSELSGGLPGVARFLT 540
            V TSIS KILAPL++R + P +  SHD DH+  V+N  C EVNQC+E S  L G+ +  T
Sbjct: 481  VGTSISSKILAPLSERVKEPEETISHDLDHRNSVDNFECSEVNQCAEPSDALLGMVKSPT 540

Query: 541  TKRSFACDGSVSSYDGMDDQFSDHDKRSLQNNHHASTFLTTVERRRREESLMNNNAVARD 600
            TK S A DGSVSSYDGMDDQ  DH + S +N H  S FLT VE  +REESL+N+NAVARD
Sbjct: 541  TKGSPAYDGSVSSYDGMDDQILDHRRCSSRNIHGTSNFLTAVEGPKREESLVNSNAVARD 600

Query: 601  SEVPIEGRNYKEISSHEKHYGIEYHERNQNDVLQHRRQDIPMQSRSRLRREKYQSKLSLL 660
            SE+PIE R+  +  S EKH+ IEYHER+QN     RR D  MQ+RSRLRREKY  KLSLL
Sbjct: 601  SEIPIEARSSWKNLSREKHHDIEYHERSQN-----RRHDFSMQNRSRLRREKYH-KLSLL 660

Query: 661  GTNHLGGYESGSASSSVFDEPHDSRMHLSE------------ILMVYELQDQLEKSCNLN 720
            G +  GGYE+GS S S+FDE  DSR+H S+            + MVYELQD+LEKSCNLN
Sbjct: 661  GRDCHGGYENGSTSGSMFDESCDSRLHSSDNFADHDEEKVRLLRMVYELQDKLEKSCNLN 720

Query: 721  GNASERLSMGSVQRDGWVPMHYNHQIPQEESWHDSEYPSYYRRNGPQINYSGQHPLSRMT 780
            GN S R+SMGS Q+D W P++Y+HQIPQEESWHDSEYPSY +RN  + +Y G + LSRMT
Sbjct: 721  GNGSGRVSMGSAQKDAWAPVYYDHQIPQEESWHDSEYPSYSQRNERRTSYPGHNSLSRMT 780

Query: 781  SAVKAVSGPQVNYPGQHSFGMEHFPENFPHSRQLLPSEHWHNQGARMPHIDNDYYSQYNS 840
            S  K VSGPQ+N      FGMEHFP+N PHS QLLPSE WHN+G+RM HID+DYYS Y+S
Sbjct: 781  SGAKTVSGPQMN-----RFGMEHFPDNCPHSMQLLPSERWHNRGSRMAHIDHDYYSSYSS 840

Query: 841  CASSPQHFL-TQLSARGIHMQSDHLNHRNHGRNHLREKNHFTKHHLRPMAGGTPFVTCYY 900
            C SSPQHFL TQLSARG HMQS HL+HRNH RN+LREKN   KHHLRPMAGG PF+TCYY
Sbjct: 841  CPSSPQHFLSTQLSARGSHMQS-HLSHRNHERNYLREKNRVVKHHLRPMAGGAPFITCYY 900

Query: 901  CLKLLQIPAEFLLVKRR-KRLKCGHCSKVLEFSLQSRTHIVPCVQSVSEPPPYEAHEHDD 960
            CLKLLQIPAEFLL+KRR  +LKCGHCSK+LEFSL+SRTHIVP     +E PP E +E DD
Sbjct: 901  CLKLLQIPAEFLLLKRRCNQLKCGHCSKILEFSLESRTHIVPYAHRAAELPPSETNECDD 960

Query: 961  YDLAMGKSGSREIGDSIVLPHSSHQDMEKEHSSQNKFAHIKKCYQSGDPSSPAYKADKLS 1016
            Y LA+GKSGSREI DSIV PH   +DM+       KF ++K  YQSG+PSS AYKADK S
Sbjct: 961  YALAIGKSGSREIDDSIVFPHPPRRDMDM------KFKNLKTGYQSGEPSSHAYKADKYS 1020

BLAST of IVF0022540 vs. NCBI nr
Match: XP_008456794.1 (PREDICTED: uncharacterized protein LOC103496635 [Cucumis melo] >XP_016901993.1 PREDICTED: uncharacterized protein LOC103496635 [Cucumis melo])

HSP 1 Score: 2023 bits (5241), Expect = 0.0
Identity = 1014/1028 (98.64%), Postives = 1015/1028 (98.74%), Query Frame = 0

Query: 1    MATGLTTKIRLVKCPRCRRLLPELPDIPVYKCGGCDAILVAKSQRDSSHNPELGSQNRDS 60
            MATGLTTKIRLVKCPRCRRLLPELPDIPVYKCGGCDAILVAKSQRDSSHNPELGSQNRDS
Sbjct: 1    MATGLTTKIRLVKCPRCRRLLPELPDIPVYKCGGCDAILVAKSQRDSSHNPELGSQNRDS 60

Query: 61   AQRREVDPPSEGNQASISNPEAVIPPHGESNLVLRNGRGTNESREWSSENLVHSALSVQQ 120
            AQRREVDPPSEGNQASISNPEAVIPPHGESNLVLRNGRGTNESREWSSENLVHSALSVQQ
Sbjct: 61   AQRREVDPPSEGNQASISNPEAVIPPHGESNLVLRNGRGTNESREWSSENLVHSALSVQQ 120

Query: 121  RNARSDDETNHENGELSDGDLLKAEEASISSSSHEAIIPSSGECVIDPDAEEDQDEFGGS 180
            RNARSDDETNHENGELSDGDLLKAEEASISSSSHEAIIPSSGECVIDPDAEEDQDEFGGS
Sbjct: 121  RNARSDDETNHENGELSDGDLLKAEEASISSSSHEAIIPSSGECVIDPDAEEDQDEFGGS 180

Query: 181  SSEQLVRRKLGERQTYARTNNDSHAVNDGPLGFPSRDRSGEEATYHDNGKLPCRDAPEQE 240
            SSEQLVRRKLGERQTYARTNNDSHAVNDGPLGFPSRDRSGEEATYHDNGKLPCRDAPEQE
Sbjct: 181  SSEQLVRRKLGERQTYARTNNDSHAVNDGPLGFPSRDRSGEEATYHDNGKLPCRDAPEQE 240

Query: 241  EYCISSPEGTIPSSEECFTDLNDVKGQKECGDISQEQLAHIKSGECLKNSGSYNESPACH 300
            EYCISSPEGTIPSSEECFTDLNDVKGQKECGDISQEQLAHIKSGECLKNSGSYNESPACH
Sbjct: 241  EYCISSPEGTIPSSEECFTDLNDVKGQKECGDISQEQLAHIKSGECLKNSGSYNESPACH 300

Query: 301  GERPSCSNQSYSRIEFASHGKANLSGRDPTKVMETSTSSHEVMTIPFSGGSVLDPNDEKD 360
            GERPSCSNQSYSRIEFASHGKANLSGRDPTKVMETSTSSHEVMTIPFSGGSVLDPNDEKD
Sbjct: 301  GERPSCSNQSYSRIEFASHGKANLSGRDPTKVMETSTSSHEVMTIPFSGGSVLDPNDEKD 360

Query: 361  QKEEGNHNEQLVQIPFTDSLACHGKQSGSSDQVSSANEVANHENDKPSSVDHSEDEIVDQ 420
            QKEEGNHNEQLVQIPFTDSLACHGKQSGSSDQVSSANEVANHENDKPSSVDHSEDEIVDQ
Sbjct: 361  QKEEGNHNEQLVQIPFTDSLACHGKQSGSSDQVSSANEVANHENDKPSSVDHSEDEIVDQ 420

Query: 421  ILLSGEESPKADSKEIHSDLESSSISNAHVVTSISPKILAPLAQRGEAPLKITSHDSDHQ 480
            ILLSGEESPKADSKEIHSDLESSSISNAHVVTSISPKILAPLAQRGEAPLKITSHDSDHQ
Sbjct: 421  ILLSGEESPKADSKEIHSDLESSSISNAHVVTSISPKILAPLAQRGEAPLKITSHDSDHQ 480

Query: 481  TPVENIACMEVNQCSELSGGLPGVARFLTTKRSFACDGSVSSYDGMDDQFSDHDKRSLQN 540
            TPVENIACMEVNQCSELSGGLPGVARFLTTKRSFACDGSVSSYDGMDDQFSDHDKRSLQN
Sbjct: 481  TPVENIACMEVNQCSELSGGLPGVARFLTTKRSFACDGSVSSYDGMDDQFSDHDKRSLQN 540

Query: 541  NHHASTFLTTVERRRREESLMNNNAVARDSEVPIEGRNYKEISSHEKHYGIEYHERNQND 600
            NHHASTFLTTVERRRREESLMNNNAVARDSEVPIEGRNYKEISSHEKHYGIEYHERNQND
Sbjct: 541  NHHASTFLTTVERRRREESLMNNNAVARDSEVPIEGRNYKEISSHEKHYGIEYHERNQND 600

Query: 601  VLQHRRQDIPMQSRSRLRREKYQSKLSLLGTNHLGGYESGSASSSVFDEPHDSRMHLSE- 660
            VLQHRRQDIPMQSRSRLRREKYQSKLSLLGTNHLGGYESGSASSSVFDEPHDSRMHLSE 
Sbjct: 601  VLQHRRQDIPMQSRSRLRREKYQSKLSLLGTNHLGGYESGSASSSVFDEPHDSRMHLSEN 660

Query: 661  -----------ILMVYELQDQLEKSCNLNGNASERLSMGSVQRDGWVPMHYNHQIPQEES 720
                       + MVYELQDQLEKSCNLNGNASERLSMGSVQRDGWVPMHYNHQIPQEES
Sbjct: 661  FVDHDEDKARLLRMVYELQDQLEKSCNLNGNASERLSMGSVQRDGWVPMHYNHQIPQEES 720

Query: 721  WHDSEYPSYYRRNGPQINYSGQHPLSRMTSAVKAVSGPQVNYPGQHSFGMEHFPENFPHS 780
            WHDSEYPSYYRRNGPQINYSGQHPLSRMTSAVKAVSGPQVNYPGQHSFGMEHFPENFPHS
Sbjct: 721  WHDSEYPSYYRRNGPQINYSGQHPLSRMTSAVKAVSGPQVNYPGQHSFGMEHFPENFPHS 780

Query: 781  RQLLPSEHWHNQGARMPHIDNDYYSQYNSCASSPQHFLTQLSARGIHMQSDHLNHRNHGR 840
            RQLLPSEHWHNQGARMPHIDNDYYSQYNSCASSPQHFLTQLSARGIHMQSDHLNHRNHGR
Sbjct: 781  RQLLPSEHWHNQGARMPHIDNDYYSQYNSCASSPQHFLTQLSARGIHMQSDHLNHRNHGR 840

Query: 841  NHLREKNHFTKHHLRPMAGGTPFVTCYYCLKLLQIPAEFLLVKRRKRLKCGHCSKVLEFS 900
            NHLREKNHFTKHHLRPMAGGTPFVTCYYCLKLLQIPAEFLLVKRRKRLKCGHCSKVLEFS
Sbjct: 841  NHLREKNHFTKHHLRPMAGGTPFVTCYYCLKLLQIPAEFLLVKRRKRLKCGHCSKVLEFS 900

Query: 901  LQSRTHIVPCVQSVSEPPPYEAHEHDDYDLAMGKSGSREIGDSIVLPHSSHQDMEKEHSS 960
            LQSRTHIVPCVQSVSEPPPYEAHEHDDYDLAMGKSGSREIGDSIVLPHSSHQDMEKEHSS
Sbjct: 901  LQSRTHIVPCVQSVSEPPPYEAHEHDDYDLAMGKSGSREIGDSIVLPHSSHQDMEKEHSS 960

Query: 961  QNKFAHIKKCYQSGDPSSPAYKADKLSSEVGKFSTKSNSPLHQLMGYSSPSQVFKGLDAP 1016
            QNKFAHIKKCYQSGDPSSPAYKADKLSSEVGKFSTKSNSPLHQLMGYSSPSQVFKGLDAP
Sbjct: 961  QNKFAHIKKCYQSGDPSSPAYKADKLSSEVGKFSTKSNSPLHQLMGYSSPSQVFKGLDAP 1020

BLAST of IVF0022540 vs. NCBI nr
Match: KAA0051961.1 (DUF3133 domain-containing protein [Cucumis melo var. makuwa] >TYK04592.1 DUF3133 domain-containing protein [Cucumis melo var. makuwa])

HSP 1 Score: 2014 bits (5219), Expect = 0.0
Identity = 1014/1039 (97.59%), Postives = 1015/1039 (97.69%), Query Frame = 0

Query: 1    MATGLTTKIRLVKCPRCRRLLPELPDIPVYKCGGCDAILV-----------AKSQRDSSH 60
            MATGLTTKIRLVKCPRCRRLLPELPDIPVYKCGGCDAILV           AKSQRDSSH
Sbjct: 1    MATGLTTKIRLVKCPRCRRLLPELPDIPVYKCGGCDAILVVLIGFILTLHSAKSQRDSSH 60

Query: 61   NPELGSQNRDSAQRREVDPPSEGNQASISNPEAVIPPHGESNLVLRNGRGTNESREWSSE 120
            NPELGSQNRDSAQRREVDPPSEGNQASISNPEAVIPPHGESNLVLRNGRGTNESREWSSE
Sbjct: 61   NPELGSQNRDSAQRREVDPPSEGNQASISNPEAVIPPHGESNLVLRNGRGTNESREWSSE 120

Query: 121  NLVHSALSVQQRNARSDDETNHENGELSDGDLLKAEEASISSSSHEAIIPSSGECVIDPD 180
            NLVHSALSVQQRNARSDDETNHENGELSDGDLLKAEEASISSSSHEAIIPSSGECVIDPD
Sbjct: 121  NLVHSALSVQQRNARSDDETNHENGELSDGDLLKAEEASISSSSHEAIIPSSGECVIDPD 180

Query: 181  AEEDQDEFGGSSSEQLVRRKLGERQTYARTNNDSHAVNDGPLGFPSRDRSGEEATYHDNG 240
            AEEDQDEFGGSSSEQLVRRKLGERQTYARTNNDSHAVNDGPLGFPSRDRSGEEATYHDNG
Sbjct: 181  AEEDQDEFGGSSSEQLVRRKLGERQTYARTNNDSHAVNDGPLGFPSRDRSGEEATYHDNG 240

Query: 241  KLPCRDAPEQEEYCISSPEGTIPSSEECFTDLNDVKGQKECGDISQEQLAHIKSGECLKN 300
            KLPCRDAPEQEEYCISSPEGTIPSSEECFTDLNDVKGQKECGDISQEQLAHIKSGECLKN
Sbjct: 241  KLPCRDAPEQEEYCISSPEGTIPSSEECFTDLNDVKGQKECGDISQEQLAHIKSGECLKN 300

Query: 301  SGSYNESPACHGERPSCSNQSYSRIEFASHGKANLSGRDPTKVMETSTSSHEVMTIPFSG 360
            SGSYNESPACHGERPSCSNQSYSRIEFASHGKANLSGRDPTKVMETSTSSHEVMTIPFSG
Sbjct: 301  SGSYNESPACHGERPSCSNQSYSRIEFASHGKANLSGRDPTKVMETSTSSHEVMTIPFSG 360

Query: 361  GSVLDPNDEKDQKEEGNHNEQLVQIPFTDSLACHGKQSGSSDQVSSANEVANHENDKPSS 420
            GSVLDPNDEKDQKEEGNHNEQLVQIPFTDSLACHGKQSGSSDQVSSANEVANHENDKPSS
Sbjct: 361  GSVLDPNDEKDQKEEGNHNEQLVQIPFTDSLACHGKQSGSSDQVSSANEVANHENDKPSS 420

Query: 421  VDHSEDEIVDQILLSGEESPKADSKEIHSDLESSSISNAHVVTSISPKILAPLAQRGEAP 480
            VDHSEDEIVDQILLSGEESPKADSKEIHSDLESSSISNAHVVTSISPKILAPLAQRGEAP
Sbjct: 421  VDHSEDEIVDQILLSGEESPKADSKEIHSDLESSSISNAHVVTSISPKILAPLAQRGEAP 480

Query: 481  LKITSHDSDHQTPVENIACMEVNQCSELSGGLPGVARFLTTKRSFACDGSVSSYDGMDDQ 540
            LKITSHDSDHQTPVENIACMEVNQCSELSGGLPGVARFLTTKRSFACDGSVSSYDGMDDQ
Sbjct: 481  LKITSHDSDHQTPVENIACMEVNQCSELSGGLPGVARFLTTKRSFACDGSVSSYDGMDDQ 540

Query: 541  FSDHDKRSLQNNHHASTFLTTVERRRREESLMNNNAVARDSEVPIEGRNYKEISSHEKHY 600
            FSDHDKRSLQNNHHASTFLTTVERRRREESLMNNNAVARDSEVPIEGRNYKEISSHEKHY
Sbjct: 541  FSDHDKRSLQNNHHASTFLTTVERRRREESLMNNNAVARDSEVPIEGRNYKEISSHEKHY 600

Query: 601  GIEYHERNQNDVLQHRRQDIPMQSRSRLRREKYQSKLSLLGTNHLGGYESGSASSSVFDE 660
            GIEYHERNQNDVLQHRRQDIPMQSRSRLRREKYQSKLSLLGTNHLGGYESGSASSSVFDE
Sbjct: 601  GIEYHERNQNDVLQHRRQDIPMQSRSRLRREKYQSKLSLLGTNHLGGYESGSASSSVFDE 660

Query: 661  PHDSRMHLSE------------ILMVYELQDQLEKSCNLNGNASERLSMGSVQRDGWVPM 720
            PHDSRMHLSE            + MVYELQDQLEKSCNLNGNASERLSMGSVQRDGWVPM
Sbjct: 661  PHDSRMHLSENFVDHDEDKARLLRMVYELQDQLEKSCNLNGNASERLSMGSVQRDGWVPM 720

Query: 721  HYNHQIPQEESWHDSEYPSYYRRNGPQINYSGQHPLSRMTSAVKAVSGPQVNYPGQHSFG 780
            HYNHQIPQEESWHDSEYPSYYRRNGPQINYSGQHPLSRMTSAVKAVSGPQVNYPGQHSFG
Sbjct: 721  HYNHQIPQEESWHDSEYPSYYRRNGPQINYSGQHPLSRMTSAVKAVSGPQVNYPGQHSFG 780

Query: 781  MEHFPENFPHSRQLLPSEHWHNQGARMPHIDNDYYSQYNSCASSPQHFLTQLSARGIHMQ 840
            MEHFPENFPHSRQLLPSEHWHNQGARMPHIDNDYYSQYNSCASSPQHFLTQLSARGIHMQ
Sbjct: 781  MEHFPENFPHSRQLLPSEHWHNQGARMPHIDNDYYSQYNSCASSPQHFLTQLSARGIHMQ 840

Query: 841  SDHLNHRNHGRNHLREKNHFTKHHLRPMAGGTPFVTCYYCLKLLQIPAEFLLVKRRKRLK 900
            SDHLNHRNHGRNHLREKNHFTKHHLRPMAGGTPFVTCYYCLKLLQIPAEFLLVKRRKRLK
Sbjct: 841  SDHLNHRNHGRNHLREKNHFTKHHLRPMAGGTPFVTCYYCLKLLQIPAEFLLVKRRKRLK 900

Query: 901  CGHCSKVLEFSLQSRTHIVPCVQSVSEPPPYEAHEHDDYDLAMGKSGSREIGDSIVLPHS 960
            CGHCSKVLEFSLQSRTHIVPCVQSVSEPPPYEAHEHDDYDLAMGKSGSREIGDSIVLPHS
Sbjct: 901  CGHCSKVLEFSLQSRTHIVPCVQSVSEPPPYEAHEHDDYDLAMGKSGSREIGDSIVLPHS 960

Query: 961  SHQDMEKEHSSQNKFAHIKKCYQSGDPSSPAYKADKLSSEVGKFSTKSNSPLHQLMGYSS 1016
            SHQDMEKEHSSQNKFAHIKKCYQSGDPSSPAYKADKLSSEVGKFSTKSNSPLHQLMGYSS
Sbjct: 961  SHQDMEKEHSSQNKFAHIKKCYQSGDPSSPAYKADKLSSEVGKFSTKSNSPLHQLMGYSS 1020

BLAST of IVF0022540 vs. NCBI nr
Match: XP_011656443.1 (uncharacterized protein LOC105435747 [Cucumis sativus] >XP_031743129.1 uncharacterized protein LOC105435747 [Cucumis sativus] >KGN45844.1 hypothetical protein Csa_005007 [Cucumis sativus])

HSP 1 Score: 1830 bits (4740), Expect = 0.0
Identity = 931/1032 (90.21%), Postives = 971/1032 (94.09%), Query Frame = 0

Query: 1    MATGLTTKIRLVKCPRCRRLLPELPDIPVYKCGGCDAILVAKSQRDSSHNPELGSQNRDS 60
            MATGLT KIRLVKCPRCRRLLPELPDIPVYKCGGCDAILVAKSQRDSSHN ELGSQNRDS
Sbjct: 1    MATGLTAKIRLVKCPRCRRLLPELPDIPVYKCGGCDAILVAKSQRDSSHNTELGSQNRDS 60

Query: 61   AQRREVDPPSEGNQASISNPEAVIPPHGESNLVLRNGRGTNESREWSSENLVHSALSVQQ 120
            AQR EV PPSEGNQASISN EAVIP HGESNLVL N R TNESREWSSENLVHSALSVQQ
Sbjct: 61   AQRHEVHPPSEGNQASISNHEAVIPRHGESNLVLSNERDTNESREWSSENLVHSALSVQQ 120

Query: 121  RNARSDDETNHENGELSDGDLLKAEEASISSSSHEAIIPSSGECVIDPDAEEDQDEFGGS 180
            RNARSDDETNHENGELSDGDLLKA+EASISSSSHEAIIPSSGECVIDPDAEEDQDEFGGS
Sbjct: 121  RNARSDDETNHENGELSDGDLLKADEASISSSSHEAIIPSSGECVIDPDAEEDQDEFGGS 180

Query: 181  SSEQLVRRKLGERQTYARTNNDSHAVNDGPLGFPSRDRSGEEATYHDNGKLPCRDAPEQE 240
            SSEQLVRRKLGE QT+AR  NDSHAVNDGPLGFPSRD SGEEAT+HDN KLPCRDAPEQE
Sbjct: 181  SSEQLVRRKLGECQTHAR--NDSHAVNDGPLGFPSRDCSGEEATHHDNRKLPCRDAPEQE 240

Query: 241  EYCISSPEGTIPSSEECFTDLNDVKGQKECGDISQEQLAHIKSGECLKNSGSYNESPACH 300
            +YC+SSPEGTIPSSEECFTD NDVKGQKECGDISQEQLAHIKS ECLK+SGSYNESPACH
Sbjct: 241  DYCMSSPEGTIPSSEECFTDPNDVKGQKECGDISQEQLAHIKSTECLKDSGSYNESPACH 300

Query: 301  GERPSCSNQSYSRIEFASHGKANLSGRDPTKVMETSTSSHEVMTIPFSGGSVLDPNDEKD 360
            GE+PSCSNQSYSRIEF+SHGKANLSG+DPTKV ETSTSSHEVMTIPFSG SV+DPNDE+D
Sbjct: 301  GEQPSCSNQSYSRIEFSSHGKANLSGQDPTKVTETSTSSHEVMTIPFSGESVVDPNDERD 360

Query: 361  QKEE-GNHNEQLVQIPFTDSLACHGKQSGSSDQVSSANEVANHENDKPSSVDHSEDEIVD 420
            QKEE GNH+EQLVQIP TDSLACHG+QSG SDQVSSANE+A+ ENDKPSSVDHSEDEIVD
Sbjct: 361  QKEEEGNHSEQLVQIPLTDSLACHGEQSGYSDQVSSANEIASRENDKPSSVDHSEDEIVD 420

Query: 421  QILLSGEESPKAD-SKEIHSDLESSSISNAHVVTSISPKILAPLAQRGEAPLKITSHDSD 480
            QILLSGEESPK D SKEI SDLESSSISNAHV TSISPKILAPLA+RGEAPLKITSHDSD
Sbjct: 421  QILLSGEESPKLDDSKEIRSDLESSSISNAHVGTSISPKILAPLAERGEAPLKITSHDSD 480

Query: 481  HQTPVENIACMEVNQCSELSGGLPGVARFLTTKRSFACDGSVSSYDGMDDQFSDHDKRSL 540
            HQ PVE+ ACMEVNQCS+LSG + G+A+ LTTK SFACDGSVSSYDGMDDQF DH KRSL
Sbjct: 481  HQIPVESFACMEVNQCSKLSGDISGMAKILTTKHSFACDGSVSSYDGMDDQFLDHHKRSL 540

Query: 541  QNNHHASTFLTTVERRRREESLMNNNAVARDSEVPIEGRNYKEISSHEKHYGIEYHERNQ 600
            QNNHHA+TFLT VERRRRE+SLMN+NAVARDSEVPIEGRN +E SSHEKHYGIEYHERNQ
Sbjct: 541  QNNHHAATFLTAVERRRREQSLMNSNAVARDSEVPIEGRNSRETSSHEKHYGIEYHERNQ 600

Query: 601  NDVLQHRRQDIPMQSRSRLRREKYQSKLSLLGTNHLGGYESGSASSSVFDEPHDSRMHLS 660
            NDVL+HRRQDIPMQSRSRLRREKYQSKLSL GTNHLGGYESGSASSSVFDEPHDSRMHLS
Sbjct: 601  NDVLEHRRQDIPMQSRSRLRREKYQSKLSLHGTNHLGGYESGSASSSVFDEPHDSRMHLS 660

Query: 661  E------------ILMVYELQDQLEKSCNLNGNASERLSMGSVQRDGWVPMHYNHQIPQE 720
            E            + MVYELQDQLEKSCNLNGNASER+SMGSVQRDGW PM+Y+HQIPQE
Sbjct: 661  ENFVDQDEDKARLLRMVYELQDQLEKSCNLNGNASERVSMGSVQRDGWAPMYYDHQIPQE 720

Query: 721  ESWHDSEYPSYYRRNGPQINYSGQHPLSRMTSAVKAVSGPQVNYPGQHSFGMEHFPENFP 780
            ESWHDSEYPS++RRNGPQINYSGQHPLSRMTSAVK VSGPQVNYPGQHSFGMEHFPENFP
Sbjct: 721  ESWHDSEYPSFHRRNGPQINYSGQHPLSRMTSAVKVVSGPQVNYPGQHSFGMEHFPENFP 780

Query: 781  HSRQLLPSEHWHNQGARMPHIDNDYYSQYNSCASSPQHFL-TQLSARGIHMQSDHLNHRN 840
            HSRQLLPSEHWHNQGARMPHIDNDYYSQY+SCASSPQHFL TQLSARGIHMQSDHLNHRN
Sbjct: 781  HSRQLLPSEHWHNQGARMPHIDNDYYSQYSSCASSPQHFLSTQLSARGIHMQSDHLNHRN 840

Query: 841  HGRNHLREKNHFTKHHLRPMAGGTPFVTCYYCLKLLQIPAEFLLVKRR-KRLKCGHCSKV 900
            HGRNHLREKNHF KHHLRPMAGG PFVTCYYCLKLLQIPAEFLLVKRR KRLKCGHCSKV
Sbjct: 841  HGRNHLREKNHFAKHHLRPMAGGAPFVTCYYCLKLLQIPAEFLLVKRRCKRLKCGHCSKV 900

Query: 901  LEFSLQSRTHIVPCVQSVSEPPPYEAHEHDDYDLAMGKSGSREIGDSIVLPHSSHQDMEK 960
            LEFSLQSRTHIVP VQSV+EPPPYEAHEHD+Y LAMGKSGSREIGD+IVLPHSSHQDMEK
Sbjct: 901  LEFSLQSRTHIVPYVQSVAEPPPYEAHEHDNYALAMGKSGSREIGDTIVLPHSSHQDMEK 960

Query: 961  EHSSQNKFAHIKKCYQSGDPSSPAYKADKLSSEVGKFSTKSNSPLHQLMGYSSPSQVFKG 1016
            EHSS+NKF HIK+ YQSGDPSSPAYKADKLSSEVGKFSTKSNSPLH+LMGYSSPSQVFKG
Sbjct: 961  EHSSRNKFEHIKRSYQSGDPSSPAYKADKLSSEVGKFSTKSNSPLHRLMGYSSPSQVFKG 1020

BLAST of IVF0022540 vs. NCBI nr
Match: XP_038884663.1 (uncharacterized protein LOC120075389 [Benincasa hispida] >XP_038884664.1 uncharacterized protein LOC120075389 [Benincasa hispida] >XP_038884665.1 uncharacterized protein LOC120075389 [Benincasa hispida])

HSP 1 Score: 1568 bits (4059), Expect = 0.0
Identity = 825/1048 (78.72%), Postives = 891/1048 (85.02%), Query Frame = 0

Query: 1    MATGLTTKIRLVKCPRCRRLLPELPDIPVYKCGGCDAILVAKSQRDSSHNPELGSQNRDS 60
            MAT LT KIRLVKCPRCRRLLPELPDIPVYKCGGCDAILVA S+RDSSHN ELGSQNR S
Sbjct: 1    MATELTAKIRLVKCPRCRRLLPELPDIPVYKCGGCDAILVATSRRDSSHNTELGSQNRGS 60

Query: 61   AQRREVDPPSEGNQASISNPEAVIPPHGESNLVLRNGRGTNESREWSSENLVHSALSVQQ 120
             QRREVDPPSE  Q  ISN EA+IP HGESNL L NGR TNES E SSENLVHS LSVQQ
Sbjct: 61   TQRREVDPPSEDTQTGISNHEAIIPHHGESNLGLNNGRDTNESGELSSENLVHSTLSVQQ 120

Query: 121  RNARSDDETNHENGELSDGDLLKAEEASISSSSHEAIIPSSGECVIDPDAEEDQDEFGGS 180
            RN R+DDE++HENGELSDGDLLKAEEASISSSSHEAIIPSSGECVIDPDAEEDQDEFGGS
Sbjct: 121  RNTRNDDESHHENGELSDGDLLKAEEASISSSSHEAIIPSSGECVIDPDAEEDQDEFGGS 180

Query: 181  SSEQLVRRKLGERQTYARTNNDSHAVNDGPLGFPSRDRSGEEATYHDNGKLPCRDAPEQE 240
            SSEQLVRRKL ERQTYAR +N S A ND P GF ++  S EE+   DNGKLPCRDAPEQE
Sbjct: 181  SSEQLVRRKLVERQTYARNDNKSDAGNDRPSGFSNQVGSEEESANRDNGKLPCRDAPEQE 240

Query: 241  EYCISSPEGTIPSSEECFTDLNDVKGQKECGDISQEQLAHIKSGECLKNSGSYNESPACH 300
            EYCISSPE TIPSS ECF D NDVKG KEC D+SQ+QLAH+K+ ECLKN+GS +ES ACH
Sbjct: 241  EYCISSPEATIPSSGECFIDPNDVKGLKECSDVSQQQLAHVKTSECLKNAGSNDESLACH 300

Query: 301  GERPSCSNQSYSRIEFASHGKANLSGRDPTKVMETSTSSHEVMTIPFSGGSVLDPNDEKD 360
              +P CSNQSYSRIEFASHG A+LSGRDPTK +ETSTS HEVMTIPFSG S LDPN EKD
Sbjct: 301  DNQPRCSNQSYSRIEFASHGNADLSGRDPTKAVETSTSIHEVMTIPFSGESNLDPNYEKD 360

Query: 361  QKEEG-NHNEQLVQIPFTD----------SLACHGKQSGSSDQVSSANEVANHENDKPSS 420
            QKEE  +H+EQLVQ  F +          S A H +QSGSSDQVSS+NEVAN END+ SS
Sbjct: 361  QKEEEESHSEQLVQTSFGEHKKNASNIDGSPARHDEQSGSSDQVSSSNEVANRENDRHSS 420

Query: 421  VDHSEDEIVDQILLSGEESPKAD-SKEIHSDLESSSISNA---HVVTSISPKILAPLAQR 480
             D SEDEI+D+ILL GEESP+AD SKEIHS+LE S ISNA    V TSIS K LAPLA++
Sbjct: 421  GDCSEDEIIDEILLLGEESPEADDSKEIHSNLERSGISNALATQVGTSISSKNLAPLAEQ 480

Query: 481  GEAPLKITSHDSDHQTPVENIACMEVNQCSELSGGLPGVARFLTTKRSFACDGSVSSYDG 540
             EAP +  SH S+ + PVEN ACMEVNQCSE SG LPG+A+FLTTKRSFACD SV SYDG
Sbjct: 481  AEAPEETISHGSNRRIPVENFACMEVNQCSEPSGDLPGMAKFLTTKRSFACDASVPSYDG 540

Query: 541  MDDQFSDHDKRSLQNNHHASTFLTTVERRRREESLMNNNAVARDSEVPIEGRNYKEISSH 600
            MDDQF DH +RSLQNNH A+ FLTTVER RREESLMN+NA+ARD E+PIEGRN +EI SH
Sbjct: 541  MDDQFLDHRRRSLQNNHEAANFLTTVERTRREESLMNSNAMARDPEIPIEGRNSQEILSH 600

Query: 601  EKHYGIEYHERNQNDVLQHRRQDIPMQSRSRLRREKYQSKLSLLGTNHLGGYESGSASSS 660
            +KHYG+EY ERNQND+LQHRRQDI MQSRSRLRREKYQSKLSLLG N  GGYESGSASSS
Sbjct: 601  KKHYGVEYRERNQNDILQHRRQDISMQSRSRLRREKYQSKLSLLGGNCQGGYESGSASSS 660

Query: 661  VFDEPHDSRMHLSE------------ILMVYELQDQLEKSCNLNGNASERLSMGSVQRDG 720
             FDEPHDSRMH S+            + MVYELQDQLEKSCNLNGNASER+SMGSVQ+DG
Sbjct: 661  AFDEPHDSRMHSSDNFLDHDEDKVKLLRMVYELQDQLEKSCNLNGNASERVSMGSVQKDG 720

Query: 721  WVPMHYNHQIPQEESWHDSEYPSYYRRNGPQINYSGQHPLSRMTSAVKAVSGPQVNYPGQ 780
            WVPM+YNHQIPQEE WHDSEYPSYYRR+GPQ NY GQH LSRMTSAVKAVSGPQVNYPGQ
Sbjct: 721  WVPMYYNHQIPQEEIWHDSEYPSYYRRSGPQTNYPGQHSLSRMTSAVKAVSGPQVNYPGQ 780

Query: 781  HSFGMEHFPENFPHSRQLLPSEHWHNQGARMPHIDNDYYSQYNSCASSPQHFL-TQLSAR 840
            HSFGMEHFPENFPHSRQ+LPSEHWHNQGA MPHID+DYYSQY+SC+SSPQHF  TQLS R
Sbjct: 781  HSFGMEHFPENFPHSRQMLPSEHWHNQGAHMPHIDHDYYSQYSSCSSSPQHFRSTQLSTR 840

Query: 841  GIHMQSDHLNHRNHGRNHLREKNHFTKHHLRPMAGGTPFVTCYYCLKLLQIPAEFLLVKR 900
            GIHMQSD+L+HRNHGRN+LREKNH  KHHLRPMAGG PF+TCYYCLKLLQIPAEFLLVKR
Sbjct: 841  GIHMQSDYLSHRNHGRNYLREKNHLAKHHLRPMAGGAPFITCYYCLKLLQIPAEFLLVKR 900

Query: 901  R-KRLKCGHCSKVLEFSLQSRTHIVPCVQSVSEPPPYEAHEHDDYDLAMGKSGSREIGDS 960
            +  RLKCG+CSKVLEFSLQSRTHIVP VQSV+EPP +EA+EHDDY LA GK GSREI DS
Sbjct: 901  QCNRLKCGNCSKVLEFSLQSRTHIVPYVQSVAEPPSHEANEHDDYSLANGKRGSREIDDS 960

Query: 961  IVLPHSSHQDMEKE---HSSQNKFAHIKKCYQSGDPSSPAYKADKLSSEVGKFSTKSNSP 1016
             VLP SS QD +KE     SQNKF HIKK YQSGDPSS AY ADKLSSEVGKFSTKSNSP
Sbjct: 961  NVLPPSSQQDTKKELGSKQSQNKFEHIKKSYQSGDPSSHAYMADKLSSEVGKFSTKSNSP 1020

BLAST of IVF0022540 vs. NCBI nr
Match: XP_022993008.1 (protein ENHANCED DISEASE RESISTANCE 4-like isoform X1 [Cucurbita maxima] >XP_022993009.1 protein ENHANCED DISEASE RESISTANCE 4-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 1192 bits (3085), Expect = 0.0
Identity = 678/1046 (64.82%), Postives = 761/1046 (72.75%), Query Frame = 0

Query: 1    MATGLTTKIRLVKCPRCRRLLPELPDIPVYKCGGCDAILVAKSQRDSSHNPELGSQNRDS 60
            MATGLT KIRLVKCPRC RLLPELPDIPVYKCGGCDAILVAKS+R+SSH+ ELGSQNRDS
Sbjct: 1    MATGLTAKIRLVKCPRCHRLLPELPDIPVYKCGGCDAILVAKSRRESSHDTELGSQNRDS 60

Query: 61   AQRREVDPPSEGNQASISNPEAVIPPHGESNLVLRNGRGTNESREWSSENLVHSALSVQQ 120
            AQRREV P S   Q SISN EA+IPPH ES   L N R  NES E S+E LVHS+LSVQ 
Sbjct: 61   AQRREVGPLSVERQPSISNHEAIIPPHDESTSGLNNDRDANESGERSNEELVHSSLSVQ- 120

Query: 121  RNARSDDETNHENGELSDGDLLKAEEASISSSSHEAIIPSSGECVIDPDAEEDQDEFGGS 180
            RNAR+DD ++HENGELSDGDLLK E ASISSSS EAII SSGE V+DPD EE QD+ G S
Sbjct: 121  RNARNDDGSHHENGELSDGDLLKVEVASISSSSPEAIISSSGEYVVDPDVEEVQDKSGDS 180

Query: 181  SSEQLVRRKLGERQTYARTNNDSHAVNDGPLGFPSRDRSGEEATYHDNGKLPCRDAPEQE 240
              EQLV +KL ERQ+YA  NN+S A N  P GF ++    EE TYH+N            
Sbjct: 181  CREQLVHKKLDERQSYAGNNNESRAGNGKPSGFSNQVYYEEECTYHENS----------- 240

Query: 241  EYCISSPEGTIPSSEECFTDLNDVKGQKECGDISQEQLAHIKSGECLKNSGSYNESPACH 300
               ISS E TIPSS +CF D ND K +KE    + EQLAHIKS ECLKN+ S  ES ACH
Sbjct: 241  ---ISSHEATIPSSGKCFIDPNDAKDRKES---AGEQLAHIKSSECLKNARSNGESQACH 300

Query: 301  GERPSCSNQSYSRIEFASHGKANLSGRDPTKVMETSTSSHEVMTIPFSGGSVLDPNDEKD 360
             E+ SCSNQSYSRIEFASHG   LSG DPTK METSTSSHEV+T+PFSG SVLD ND+KD
Sbjct: 301  DEQLSCSNQSYSRIEFASHGNDELSGPDPTKAMETSTSSHEVITVPFSGESVLDQNDDKD 360

Query: 361  QKEE-GNHNEQLVQIPFTDSLACHGKQSGSSDQVSSANEVANHENDKPSSVDHSEDEIVD 420
            QKEE  NH+EQLVQ+                    S NE  N ENDK SS D SEDE +D
Sbjct: 361  QKEEEDNHSEQLVQV--------------------SLNEGVNRENDKLSSRDPSEDERLD 420

Query: 421  QILLSGEESPKADS-KEIHSDLESSSISNA-----------HVVTSISPKILAPLAQRGE 480
            +ILLSGE SP+AD  KEIHS+LE+S IS A            V TSIS K L+PLA+R E
Sbjct: 421  EILLSGEGSPEADDRKEIHSNLETSGISIAVATSSIIYPDTKVGTSISSKNLSPLAERAE 480

Query: 481  APLKITSHDSDHQTPVENIACMEVNQCSELSGGLPGVARFLTTKRSFACDGSVSSYDGMD 540
            AP +  SHD DH  PVEN  CMEVN+CSE SG LPG+ + LTTK  FA       YDGMD
Sbjct: 481  APEETISHDFDHHIPVENFGCMEVNRCSEPSGALPGMVKSLTTKSYFA-------YDGMD 540

Query: 541  DQFSDHDKRSLQNNHHASTFLTTVERRRREESLMNNNAVARDSEVPIEGRNYKEISSHEK 600
            D+FSD  +RSL+N H A  FL+TVE  RR+ESLM NNAV RDS++PIE  + +EI  HEK
Sbjct: 541  DRFSDRHRRSLKNTHEAVNFLSTVEGPRRQESLMKNNAVVRDSDIPIEAGSSQEILPHEK 600

Query: 601  HYGIEYHERNQNDVLQHRRQDIPMQSRSRLRREKYQSKLSLLGTNHLGGYESGSASSSVF 660
            HY I Y ERNQND+LQHRR  I MQSRSRLRREKYQSK SLL +N  G  E+GSAS+S  
Sbjct: 601  HYDIAYLERNQNDMLQHRRHGISMQSRSRLRREKYQSKSSLLRSNLEGDRENGSASNSTI 660

Query: 661  DEPHDSRMHLSE------------ILMVYELQDQLEKSCNLNGNASERLSMGSVQRDGWV 720
            + PHDSRMH SE            + MVYELQDQLEKSCNLN N   R+S GSVQ+D W 
Sbjct: 661  NGPHDSRMHSSENFVDPDEEKVRLLRMVYELQDQLEKSCNLNTNTGGRVSTGSVQKDTWA 720

Query: 721  PMHYNHQIPQEESWHDSEYPSYYRRNGPQINYSGQHPLSRMTSAVKAVSGPQVNYPGQHS 780
            PM+ N QI QEESWH+SE+P Y+RR+GPQ NY GQH LSRMTSA KAVSGPQ N      
Sbjct: 721  PMYNNQQISQEESWHESEHPYYFRRSGPQTNYPGQHSLSRMTSAAKAVSGPQNN-----C 780

Query: 781  FGMEHFPENFPHSRQLLPSEHWHNQGARMPHIDNDYYSQYNSCASSPQHF-LTQLSARGI 840
            F ME FP+N P   Q   SE WHN+GARM H+D+DYYSQYNSCASSPQHF  TQL ARGI
Sbjct: 781  FSMEQFPDNIPPLMQRRSSEVWHNRGARMAHMDHDYYSQYNSCASSPQHFHCTQLPARGI 840

Query: 841  HMQSDHLNHRNHGRNHLREKNHFTKHHLRPMAGGTPFVTCYYCLKLLQIPAEFLLVKRR- 900
             MQSD  +H N   N+LREK H  KHHLRPMAGG PF+TCY+CLKLLQIPAEFLLVKR  
Sbjct: 841  QMQSDQSSHGNLEMNYLREKRHLAKHHLRPMAGGAPFITCYHCLKLLQIPAEFLLVKRPC 900

Query: 901  KRLKCGHCSKVLEFSLQSRTHIVPCVQSVSEPPPYEAHEHDDYDLAMGKSGSREIGDSIV 960
             RLKCGHCSKVLEFSL +RTHIVP +Q+V+EPPPYEA EH+DY L +G  GSREI DSIV
Sbjct: 901  NRLKCGHCSKVLEFSLWNRTHIVPYIQNVAEPPPYEAEEHNDYALTIGNRGSREIDDSIV 960

Query: 961  LPHSSHQDMEKEHSSQ---NKFAHIKKCYQSGDPSSPAYKADKLSSEVGKFSTKSNSPLH 1016
            LPHSSH+ MEKE SS+   NKF  +KK YQSGDP          SS+VGK ST+S SPLH
Sbjct: 961  LPHSSHRGMEKELSSKWSSNKFESLKKSYQSGDP----------SSKVGKLSTESVSPLH 986

BLAST of IVF0022540 vs. TAIR 10
Match: AT5G05190.1 (Protein of unknown function (DUF3133) )

HSP 1 Score: 94.0 bits (232), Expect = 7.6e-19
Identity = 92/291 (31.62%), Postives = 123/291 (42.27%), Query Frame = 0

Query: 750  YPGQHSF-------GMEH-FPENFPHSRQLLPSEHWHNQGAR----MPHIDNDYYS---- 809
            YP  HS        GM H F +   H+    P      QG+R    M      +YS    
Sbjct: 372  YPRAHSLQMPSYAGGMNHDFVDYMYHNN---PRARGQGQGSRISGEMGRNHGGWYSGQLH 431

Query: 810  -QYNSCASSPQHFLTQLSARG------IHMQSDHLNHRNHGRNH------LREKNHFTKH 869
              Y+S ++SPQ  + Q           +    DH  +R+ G +H      LRE+    K 
Sbjct: 432  NSYSSYSASPQRPMEQPEYHPRWRREIVSDVEDHQRNRHAGHHHELQTRRLRERQRVAKR 491

Query: 870  HLRPMAGGTPFVTCYYCLKLLQIPAEFLLVKRRKR-LKCGHCSKVLEFSLQSRTHIVPCV 929
            H+RP AGG PFV+CY C + LQ+P +FL+ KR+   L+CG C+ VL FSLQSR H+VP V
Sbjct: 492  HVRPTAGGAPFVSCYSCSENLQLPVDFLIFKRKHHLLRCGTCTTVLRFSLQSRNHLVPAV 551

Query: 930  QSVSEPPPYEAHEHDDYDLAMGKSGSREIGDSIVLPHSSHQDMEKEHSSQNKFAHIKKCY 989
                                                  +H      +S+    + I K  
Sbjct: 552  --------------------------------------THDINANRNSNSTSESPIDKA- 611

Query: 990  QSGDPSSPAYKADKLSSEV--GKFSTKSNSPLHQLMGYSSPSQVFKGLDAP 1009
                PS P    +KL S V   +      SPLH+LMGYS+ SQVFK    P
Sbjct: 612  ----PSKP----EKLRSSVQDEELPVARGSPLHRLMGYSTVSQVFKVSQRP 612

HSP 2 Score: 63.5 bits (153), Expect = 1.1e-09
Identity = 49/149 (32.89%), Postives = 72/149 (48.32%), Query Frame = 0

Query: 1   MATGLTTKIRLVKCPRCRRLLPELPDIPVYKCGGCDAILVAKSQRDS-SHNPELGSQNRD 60
           MA+    KIRLV+CP+C ++L E  D+PVY+CGGC AIL AK +  + S  P  G   R 
Sbjct: 1   MASQTGQKIRLVRCPKCLKILQEDEDVPVYQCGGCSAILQAKRRNIAPSSTPSAGETERA 60

Query: 61  SAQRREVDPPSEGNQASISNPEAVIPPHGESNLVLRNGRGTNESREWSSENLVHSALSVQ 120
            A   +   P   N +S S  + V+P     ++     +G N S E + + L    LS  
Sbjct: 61  QANEPQ-SVPETNNVSSSSGQDTVLPSSPGRSVDQEYEKGRNASMESTEKELDDLELS-- 120

Query: 121 QRNARSDDETNHENGELSDGDLLKAEEAS 149
             N    +E   +   L D +  + E+ S
Sbjct: 121 --NGDGTNEIQEQECSLGDSEKNEREDNS 144

BLAST of IVF0022540 vs. TAIR 10
Match: AT2G46380.1 (Protein of unknown function (DUF3133) )

HSP 1 Score: 64.7 bits (156), Expect = 4.9e-10
Identity = 51/174 (29.31%), Postives = 81/174 (46.55%), Query Frame = 0

Query: 842 LRPMAGGTPFVTCYYCLKLLQIPAEFLLVKRRK-RLKCGHCSKVLEFSLQSRTHIVPCVQ 901
           ++P+AGG PF+ C  C KLL++P +     R+K R++CG CS V+++S   +  I+    
Sbjct: 378 IQPVAGGAPFINCINCFKLLKLPEKIDSATRKKQRMRCGACSCVIDYSFVDKKLILSTDP 437

Query: 902 SVSEPPPYEAHEH-----------DDYDLAMGK-----SGSREIGDSIVLPHSSHQDMEK 961
           + +  P  E H             DDYD A  +      GS ++  ++VL  ++ Q+M  
Sbjct: 438 ASARKP--ETHSRLRWETTANFSSDDYDNAAYEFHAMDRGSADVSTALVLISNTAQEM-- 497

Query: 962 EHSSQNKFAHIKKCYQSGDPSSPAYKADKLSSEVGKF-STKSNSPLHQLMGYSS 998
                          Q  D +SP+   D+LSS+          SPLH+   YSS
Sbjct: 498 ---------------QITDATSPSVSEDELSSDSSTVRKVNLASPLHKQFEYSS 532

HSP 2 Score: 52.4 bits (124), Expect = 2.5e-06
Identity = 21/44 (47.73%), Postives = 30/44 (68.18%), Query Frame = 0

Query: 1  MATGLTTKIRLVKCPRCRRLLPELPDIPVYKCGGCDAILVAKSQ 45
          MA   T + RLV+CP+C+ LL E  D P ++CGGC  +L AK++
Sbjct: 1  MAESSTIEARLVRCPKCKNLLQEPKDCPFFQCGGCGTVLCAKNK 44

BLAST of IVF0022540 vs. TAIR 10
Match: AT3G56410.1 (Protein of unknown function (DUF3133) )

HSP 1 Score: 63.9 bits (154), Expect = 8.4e-10
Identity = 57/202 (28.22%), Postives = 93/202 (46.04%), Query Frame = 0

Query: 699 YNHQIPQEESWHDSEYPSYYRRNGPQINYSGQHPLSRMTSAVKAVSGPQVNYPGQHSFGM 758
           Y +  P   S+  +   SYY +   Q     +    + +S    V+ P   +PG+ S G 
Sbjct: 187 YGYSSPFHGSYVSASEQSYYHQQPNQFEQYSREGWFQESS----VASP-TRFPGETSDGK 246

Query: 759 EHFPENFPHSRQLLPSEHWHNQGARMPHIDNDYYSQYNSCASSPQHFLTQLSARGIHMQS 818
            +   +      L     +    +  PH  +  YS+ +   ++  H  T         +S
Sbjct: 247 YYHRSSQSQLHDLQYHNLYEPSRSETPH--HSVYSERSYVPAAAPHRSTYSEHSVGISKS 306

Query: 819 DHLNHRNHGRN---HLREKNHFTKHHLRPMAGGTPFVTCYYCLKLLQIPAEFLLVKR-RK 878
           D  + ++  RN   ++RE+N   K H+ P AGG PF TC YCL+LLQ+P      KR R 
Sbjct: 307 DTSSEKSILRNKKRYVRERNPVVKRHILPSAGGAPFATCSYCLELLQLPQVSPQGKRQRY 366

Query: 879 RLKCGHCSKVLEFSLQSRTHIV 897
           +++CG CS VL+FS++ +   V
Sbjct: 367 QVRCGSCSGVLKFSIREKADTV 381

HSP 2 Score: 51.2 bits (121), Expect = 5.6e-06
Identity = 49/154 (31.82%), Postives = 75/154 (48.70%), Query Frame = 0

Query: 4   GLTTKIRLVKCPRCRRLLPELPDIPVYKCGGCDAILVAKSQRDSSHN-----PE--LGSQ 63
           GL+++ R+V+CP+C +LL E  D   YKCGGCD+IL AK      ++     PE  L SQ
Sbjct: 12  GLSSQSRIVRCPKCHKLLQEPLDATSYKCGGCDSILHAKRWEPDGNDHTNTIPEALLSSQ 71

Query: 64  NRDSAQRREVDPPSEGNQASISNPEAVIPPHGESNLVLRNGRGTNESREWSSENLVHSAL 123
           NR  +   EV+ P +G++  +         H E N    +   T+  R +  E +     
Sbjct: 72  NRSLS--AEVESPEDGSRTPMRT------THREYN----SRPSTSVERGYHPETVYKPET 131

Query: 124 SVQQRN--ARSDDETNHENGELSDGDLLKAEEAS 149
           S  +R    R+DD +     E  D D+  +E +S
Sbjct: 132 SDIRREWMRRTDDFS-----ETGDSDVFTSERSS 148

BLAST of IVF0022540 vs. TAIR 10
Match: AT3G56410.2 (Protein of unknown function (DUF3133) )

HSP 1 Score: 63.9 bits (154), Expect = 8.4e-10
Identity = 57/202 (28.22%), Postives = 93/202 (46.04%), Query Frame = 0

Query: 699 YNHQIPQEESWHDSEYPSYYRRNGPQINYSGQHPLSRMTSAVKAVSGPQVNYPGQHSFGM 758
           Y +  P   S+  +   SYY +   Q     +    + +S    V+ P   +PG+ S G 
Sbjct: 234 YGYSSPFHGSYVSASEQSYYHQQPNQFEQYSREGWFQESS----VASP-TRFPGETSDGK 293

Query: 759 EHFPENFPHSRQLLPSEHWHNQGARMPHIDNDYYSQYNSCASSPQHFLTQLSARGIHMQS 818
            +   +      L     +    +  PH  +  YS+ +   ++  H  T         +S
Sbjct: 294 YYHRSSQSQLHDLQYHNLYEPSRSETPH--HSVYSERSYVPAAAPHRSTYSEHSVGISKS 353

Query: 819 DHLNHRNHGRN---HLREKNHFTKHHLRPMAGGTPFVTCYYCLKLLQIPAEFLLVKR-RK 878
           D  + ++  RN   ++RE+N   K H+ P AGG PF TC YCL+LLQ+P      KR R 
Sbjct: 354 DTSSEKSILRNKKRYVRERNPVVKRHILPSAGGAPFATCSYCLELLQLPQVSPQGKRQRY 413

Query: 879 RLKCGHCSKVLEFSLQSRTHIV 897
           +++CG CS VL+FS++ +   V
Sbjct: 414 QVRCGSCSGVLKFSIREKADTV 428

HSP 2 Score: 51.2 bits (121), Expect = 5.6e-06
Identity = 49/154 (31.82%), Postives = 75/154 (48.70%), Query Frame = 0

Query: 4   GLTTKIRLVKCPRCRRLLPELPDIPVYKCGGCDAILVAKSQRDSSHN-----PE--LGSQ 63
           GL+++ R+V+CP+C +LL E  D   YKCGGCD+IL AK      ++     PE  L SQ
Sbjct: 59  GLSSQSRIVRCPKCHKLLQEPLDATSYKCGGCDSILHAKRWEPDGNDHTNTIPEALLSSQ 118

Query: 64  NRDSAQRREVDPPSEGNQASISNPEAVIPPHGESNLVLRNGRGTNESREWSSENLVHSAL 123
           NR  +   EV+ P +G++  +         H E N    +   T+  R +  E +     
Sbjct: 119 NRSLS--AEVESPEDGSRTPMRT------THREYN----SRPSTSVERGYHPETVYKPET 178

Query: 124 SVQQRN--ARSDDETNHENGELSDGDLLKAEEAS 149
           S  +R    R+DD +     E  D D+  +E +S
Sbjct: 179 SDIRREWMRRTDDFS-----ETGDSDVFTSERSS 195

BLAST of IVF0022540 vs. TAIR 10
Match: AT3G61670.1 (Protein of unknown function (DUF3133) )

HSP 1 Score: 48.5 bits (114), Expect = 3.6e-05
Identity = 29/86 (33.72%), Postives = 46/86 (53.49%), Query Frame = 0

Query: 6  TTKIRLVKCPRCRRLLPELPDIPVYKCGGCDAILVAKSQRDSSHNPELGSQNRDSAQRRE 65
          +TK+RLV+CP+C  LL E  D P ++CGGC  +L AK++           +  DS   + 
Sbjct: 4  STKVRLVRCPKCENLLSEPEDSPFFQCGGCFTVLRAKTK----------EREADSVSVKS 63

Query: 66 VDPPSEGNQASISNPEAVIPPHGESN 92
          V+  ++    S S+PE  I    E++
Sbjct: 64 VEDTAK--PVSASSPEKAILDSSETS 77

HSP 2 Score: 43.9 bits (102), Expect = 9.0e-04
Identity = 99/461 (21.48%), Postives = 189/461 (41.00%), Query Frame = 0

Query: 557 EESLMNNNAVARDSEVPIEGRNYK------EISSHEKHYGIEYHERNQNDVLQHRRQDIP 616
           E+++++++  + DS+VP    ++       E     K   +E   R+   +L   + D+ 
Sbjct: 67  EKAILDSSETSSDSDVPSLRHHHNVVPVDVESDPCSKPSSLEQGNRS---ILLGDKDDLK 126

Query: 617 MQS---------RSRLRREK---YQSKLSLLGTNHLGGYESGSASSSVF-DEPHDSRMHL 676
            QS         R R R  K    QS ++ L T+     E  S+S++ F D   + + HL
Sbjct: 127 SQSGRQQDSGWDRFRKRTTKRCDSQSVINRLSTSRHPCDEGTSSSANYFPDSLLEFQKHL 186

Query: 677 SE-------------ILMVYELQDQLEKSCNLNGNASERLSMGSVQRDGW---VPMHY-- 736
            +             +  + ++++QL +SCN+  + S+  +  S    G     PM +  
Sbjct: 187 KDQSNEAIEQDRAGLLRQLEKIKEQLVQSCNVATDKSKEQAPSSSSASGLNKAPPMRFHS 246

Query: 737 --NHQIPQEESWHDSEYP-SYYRRNGPQINYSGQHPLSRMTSAVKAVS-GPQVNYPGQHS 796
             NH +     +H  ++P +    N   +++S  HP             GP   + GQ+ 
Sbjct: 247 TGNHAVGGPSYYHQPQFPYNNNNINEAPMHHSLMHPSYGDPHRFPIHGRGPHPYFSGQYV 306

Query: 797 FG-------MEHFPE---NFPHSR----QLLPSEHWHNQGARMPH--IDNDYYSQYNSCA 856
                     + +P+   +F HS         +++W      +P    +  +Y   +   
Sbjct: 307 GNNNNGHDLFDAYPQQNGHFHHSSCSCYHCYDNKYWRGSAPVVPDAPYNAGFYPHESVMG 366

Query: 857 SSPQHFLTQLSARGI----HMQSDHLNHRNHGRNHLREKNHFT---KHHLRPMAGGTPFV 916
            +P H      +RG+       S+  + +    + +R           H+RP+AGG PF+
Sbjct: 367 FAPPHNPRTYGSRGLQPHGRWPSNFSDAQMDALSRIRPPKVVLSGGSRHIRPLAGGAPFI 426

Query: 917 TCYYCLKLLQIPAE-FLLVKRRKRLKCGHCSKVLEFSLQSRTHIVPCVQSVS---EPPPY 948
           TC  C +LLQ+P +     K++++++CG CS +++ S+ +   ++    + +   E    
Sbjct: 427 TCQNCFELLQLPKKPEAGTKKQQKVRCGACSCLIDLSVVNNKFVLSTNTASTRQGEARVA 486