IVF0022449 (gene) Melon (IVF77) v1

BLAST of IVF0022449 vs. ExPASy Swiss-Prot
Match: O22256 (Probable pectinesterase/pectinesterase inhibitor 20 OS=Arabidopsis thaliana OX=3702 GN=PME20 PE=2 SV=2)

HSP 1 Score: 626.3 bits (1614), Expect = 3.2e-178
Identity = 318/548 (58.03%), Postives = 403/548 (73.54%), Query Frame = 0

Query: 3   YLDSIYDSLTNIFPTKAICKLTSNPSYCITVLKQSRDGNIYDSGRFSIRRSLSKASRFLD 62
           ++ +   ++ N      IC+   +PSYC +VL  ++ G+IY  GR S+RRSLS+A RF+ 
Sbjct: 21  FISAQIPAIGNATSPSNICRFAPDPSYCRSVL-PNQPGDIYSYGRLSLRRSLSRARRFIS 80

Query: 63  LIEKHL-QNSSTLPKSIIGALKDCQYLAQLNMNFLSNSFRAVNGTDRKLTYSKADYIQSL 122
           +I+  L +      KS +GAL+DC++LA L M++L +S +  + T + L+ S+A+ + + 
Sbjct: 81  MIDAELDRKGKVAAKSTVGALEDCKFLASLTMDYLLSSSQTADST-KTLSLSRAEDVHTF 140

Query: 123 LSAILTNIDTCLDGLNTVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPRWNRNRTL 182
           LSA +TN  TCL+GL + AS + L  D    L + TK Y +SL LF+KGWVPR  R+R +
Sbjct: 141 LSAAITNEQTCLEGLKSTASENGLSGD----LFNDTKLYGVSLALFSKGWVPRRQRSRPI 200

Query: 183 EHPG---KKHLQFRKGPLPLRMSRHDRAVYNSVANRRKLSSSSDDGVLVNGIAVVSQDGE 242
             P    KK   FR G LPL+M+   RAVYN+V  RRKL  S  D V V+ I  V Q+G 
Sbjct: 201 WQPQARFKKFFGFRNGKLPLKMTERARAVYNTV-TRRKLLQSDADAVQVSDIVTVIQNGT 260

Query: 243 GDFLNITDAINAAPNNSLAANGYFLIYITAGVYQEYVSVPSKKKYLLMIGDGINQTIITG 302
           G+F  I  AI AAPN +  +NGYFLIY+TAG+Y+EYV VP  K+Y++MIGDGINQT+ITG
Sbjct: 261 GNFTTINAAIAAAPNKTDGSNGYFLIYVTAGLYEEYVEVPKNKRYVMMIGDGINQTVITG 320

Query: 303 NRSVADGWTTFNSATFAVAAEGFMAVNITFQNTAGAIKGQAVALRSGADMCVFYSCSFEG 362
           NRSV DGWTTFNSATF ++   F+ VNIT +NTAG  KGQAVALRSG D+ VFYSCSFE 
Sbjct: 321 NRSVVDGWTTFNSATFILSGPNFIGVNITIRNTAGPTKGQAVALRSGGDLSVFYSCSFEA 380

Query: 363 YQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPGPRQANMITAQGRSDP 422
           YQDTLYTHSLRQF+RECD+YGTVDFIFGNAAVV QNCN+YPR P   Q+N +TAQGR+DP
Sbjct: 381 YQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCNLYPRQPRKGQSNEVTAQGRTDP 440

Query: 423 NQNTGTSIHNCTIQATPDLAASSSHMNKTYLGRPWKQYSRTVYMQTFIDGFVNPKGWDPW 482
           NQNTGT+IH CTI+   DL A+S++  KTYLGRPWK+YSRTV MQT+IDGF+ P GW+ W
Sbjct: 441 NQNTGTAIHGCTIRPADDL-ATSNYTVKTYLGRPWKEYSRTVVMQTYIDGFLEPSGWNAW 500

Query: 483 SGEY-LSTLYYGEYNNTGGGSDTKNRVIWPGYHVINNATDAANFTISNFLVGDVWLPPTG 542
           SG++ LSTLYY EYNNTG GSDT NRV WPGYHVI NATDA+NFT++NFLVG+ W+  TG
Sbjct: 501 SGDFALSTLYYAEYNNTGPGSDTTNRVTWPGYHVI-NATDASNFTVTNFLVGEGWIGQTG 559

Query: 543 VPYTGGFV 546
           VP+ GG +
Sbjct: 561 VPFVGGLI 559

BLAST of IVF0022449 vs. ExPASy Swiss-Prot
Match: Q9SRX4 (Probable pectinesterase/pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PME7 PE=2 SV=1)

HSP 1 Score: 620.2 bits (1598), Expect = 2.3e-176
Identity = 322/568 (56.69%), Postives = 410/568 (72.18%), Query Frame = 0

Query: 3   YLDSIYDSLTNIFPTKAICKLTSNPSYCITVLKQSRDGNIYDSGRFSIRRSLSKASRFLD 62
           +L S+  S   +  +  ICK T +P YC +V   S+ GN+   G FSIR+SLS++ +F+ 
Sbjct: 14  FLQSVLASSQTLSNSSTICKTTPDPKYCKSVFPHSQ-GNVQQYGCFSIRKSLSQSRKFIR 73

Query: 63  LIEKHLQNSSTLPK-SIIGALKDCQYLAQLNMNFLSNSFRAVNGTDRK-----LTYSKAD 122
            ++++++ ++ L + ++I AL+DC++LA L M++L  SF  VN T  K     L++ KAD
Sbjct: 74  TVDRYIKRNAHLSQPAVIRALQDCRFLAGLTMDYLLTSFETVNDTSAKTSFKPLSFPKAD 133

Query: 123 YIQSLLSAILTNIDTCLDGLNTVASGS---SLEKDLLAPLIDCTKSYSLSLDLFTKGWVP 182
            IQ+LLSA LTN  TCL+GL T AS S   ++   +  PL++ TK   +SL LFTKGWVP
Sbjct: 134 DIQTLLSAALTNEQTCLEGLTTAASYSATWTVRTGVALPLVNDTKLLGVSLALFTKGWVP 193

Query: 183 R--------WNRNRTLEHPGKKHLQ-FRKGPLPLRMSRHDRAVYNSVANRRKLSSSS--- 242
           +        W + R+      K  + FR G LPL+M+   +AVY S++ R+     S   
Sbjct: 194 KKKKRAGFAWAQPRSGSSTHTKPFRLFRNGALPLKMTEKTKAVYESLSRRKLADGDSNGD 253

Query: 243 -DDG--VLVNGIAVVSQDGEGDFLNITDAINAAPNNSLAANGYFLIYITAGVYQEYVSVP 302
            DDG  VL++ I  VSQDG G+F NIT A+ AAPNN+  + G+FLIY+TAG+Y+EY+S+ 
Sbjct: 254 GDDGSMVLISDIVTVSQDGTGNFTNITAAVAAAPNNTDGSAGFFLIYVTAGIYEEYISIA 313

Query: 303 SKKKYLLMIGDGINQTIITGNRSVADGWTTFNSATFAVAAEGFMAVNITFQNTAGAIKGQ 362
             K+Y++MIGDGINQT++TGNRSV DGWTTFNSATFAV A  F+AVNITF+NTAG  K Q
Sbjct: 314 KNKRYMMMIGDGINQTVVTGNRSVVDGWTTFNSATFAVTAPNFVAVNITFRNTAGPEKHQ 373

Query: 363 AVALRSGADMCVFYSCSFEGYQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIY 422
           AVALRSGAD  +FYSCSFE YQDTLYTHSLRQF+RECD+YGTVDFIFGNAAVVFQNCN+Y
Sbjct: 374 AVALRSGADFSIFYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVFQNCNLY 433

Query: 423 PRLPGPRQANMITAQGRSDPNQNTGTSIHNCTIQATPDLAASSSHMNKTYLGRPWKQYSR 482
           PR P P Q N ITAQGRSDPNQNTGTSI NCTI+   DL  SS++  KTYLGRPWK+YSR
Sbjct: 434 PRKPMPNQFNAITAQGRSDPNQNTGTSIQNCTIKPADDL-VSSNYTVKTYLGRPWKEYSR 493

Query: 483 TVYMQTFIDGFVNPKGWDPWSGEY-LSTLYYGEYNNTGGGSDTKNRVIWPGYHVINNATD 542
           TVYMQ++IDGFV P GW  W+G++ LSTLYY EYNNTG GS+T NRV WPGYHVI N+TD
Sbjct: 494 TVYMQSYIDGFVEPVGWREWNGDFALSTLYYAEYNNTGPGSNTTNRVTWPGYHVI-NSTD 553

Query: 543 AANFTISNFLVGDVWLPPTGVPYTGGFV 546
           AANFT++   +   W+  TGVPYT G +
Sbjct: 554 AANFTVTGLFIEADWIWKTGVPYTSGLI 578

BLAST of IVF0022449 vs. ExPASy Swiss-Prot
Match: Q8RXK7 (Probable pectinesterase/pectinesterase inhibitor 41 OS=Arabidopsis thaliana OX=3702 GN=PME41 PE=2 SV=2)

HSP 1 Score: 618.6 bits (1594), Expect = 6.7e-176
Identity = 313/561 (55.79%), Postives = 412/561 (73.44%), Query Frame = 0

Query: 4   LDSIYDSLTNIFPTKA---ICKLTSNPSYCITVLKQSRDGNIYDSGRFSIRRSLSKASRF 63
           L +++ +   + P+ +   ICK T +P +C +V  Q+  G++ + GRFS+R+SL+++ +F
Sbjct: 15  LQTLFIASQTLLPSNSSSTICKTTPDPKFCKSVFPQTSQGDVREYGRFSLRKSLTQSRKF 74

Query: 64  LDLIEKHL-QNSSTLPKSIIGALKDCQYLAQLNMNFLSNSFRAVNGT--DRKLTYSKADY 123
              I+++L +N++ L +S +GAL+DC+YLA L  ++L  SF  VN T   + L++SKAD 
Sbjct: 75  TRTIDRYLKRNNALLSQSAVGALQDCRYLASLTTDYLITSFETVNITTSSKTLSFSKADE 134

Query: 124 IQSLLSAILTNIDTCLDGLNTVASGS-SLEKDLLAPLIDCTKSYSLSLDLFTKGWVPR-- 183
           IQ+LLSA LTN  TCLDG+NT AS S ++   +  PLI+ TK +S+SL LFTKGWVP+  
Sbjct: 135 IQTLLSAALTNEQTCLDGINTAASSSWTIRNGVALPLINDTKLFSVSLALFTKGWVPKKK 194

Query: 184 -------WNRNRTLEHPGKKHLQFRKGPLPLRMSRHDRAVYNSVANRRKLSSSSDD--GV 243
                  W   +      K    FR G LPL+M+ H RAVY S+ +RRKL+   +D   V
Sbjct: 195 KQVASYSWAHPKNTHSHTKPFRHFRNGALPLKMTEHTRAVYESL-SRRKLADDDNDVNTV 254

Query: 244 LVNGIAVVSQDGEGDFLNITDAINAAPNNSLAANGYFLIYITAGVYQEYVSVPSKKKYLL 303
           LV+ I  V+Q+G G+F  IT+A+N+APN +    GYF+IY+T+GVY+E V +   K+YL+
Sbjct: 255 LVSDIVTVNQNGTGNFTTITEAVNSAPNKTDGTAGYFVIYVTSGVYEENVVIAKNKRYLM 314

Query: 304 MIGDGINQTIITGNRSVADGWTTFNSATFAVAAEGFMAVNITFQNTAGAIKGQAVALRSG 363
           MIGDGIN+T++TGNR+V DGWTTFNSATFAV +  F+AVN+TF+NTAG  K QAVA+RS 
Sbjct: 315 MIGDGINRTVVTGNRNVVDGWTTFNSATFAVTSPNFVAVNMTFRNTAGPEKHQAVAMRSS 374

Query: 364 ADMCVFYSCSFEGYQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPGPR 423
           AD+ +FYSCSFE YQDTLYTHSLRQF+RECDIYGTVDFIFGNAAVVFQ+CN+YPR P   
Sbjct: 375 ADLSIFYSCSFEAYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVFQDCNLYPRQPMQN 434

Query: 424 QANMITAQGRSDPNQNTGTSIHNCTIQATPDLAASSSHMNKTYLGRPWKQYSRTVYMQTF 483
           Q N ITAQGR+DPNQNTG SIHNCTI+   DL  SS++  KTYLGRPWK+YSRTV+MQ++
Sbjct: 435 QFNAITAQGRTDPNQNTGISIHNCTIKPADDL-VSSNYTVKTYLGRPWKEYSRTVFMQSY 494

Query: 484 IDGFVNPKGWDPWSGEY-LSTLYYGEYNNTGGGSDTKNRVIWPGYHVINNATDAANFTIS 543
           ID  V P GW  W+G++ LSTLYY EYNNTG GS T +RV+WPGYHVI N+TDA NFT+ 
Sbjct: 495 IDEVVEPVGWREWNGDFALSTLYYAEYNNTGSGSSTTDRVVWPGYHVI-NSTDANNFTVE 554

Query: 544 NFLVGDVWLPPTGVPYTGGFV 546
           NFL+GD W+  +GVPY  G +
Sbjct: 555 NFLLGDGWMVQSGVPYISGLL 572

BLAST of IVF0022449 vs. ExPASy Swiss-Prot
Match: Q94CB1 (Probable pectinesterase/pectinesterase inhibitor 25 OS=Arabidopsis thaliana OX=3702 GN=PME25 PE=2 SV=1)

HSP 1 Score: 460.3 bits (1183), Expect = 3.0e-128
Identity = 254/540 (47.04%), Postives = 344/540 (63.70%), Query Frame = 0

Query: 21  CKLTSNPSYCITVLK--QSRDGNIYDSGRFSIRRSLSKASRFLDLIEKHLQNSSTLPKSI 80
           CK T  P  C T+L   +S   + Y  G+F+I++ L +ASR   +I  + +   + P S 
Sbjct: 83  CKSTPYPKLCRTILNAVKSSPSDPYRYGKFTIKQCLKQASRLSKVITSYARRVESKPGSA 142

Query: 81  ----IGALKDCQYLAQLNMNFLSNSFRAVNGTDRKLTYSKADYIQSLLSAILTNIDTCLD 140
               IGA+ DC  L++L++N+L      +  T + +T +  +++ SLLS ++TN  TCLD
Sbjct: 143 TAEEIGAVADCGELSELSVNYLETVTTELK-TAQVMTAALVEHVNSLLSGVVTNQQTCLD 202

Query: 141 GLNTVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPRWNRNRTLEHP--GKKHLQFR 200
           GL  V + S     + +P+ + T+ YS+SL L +        R +  +    G  +  +R
Sbjct: 203 GL--VEAKSGFAAAIGSPMGNLTRLYSISLGLVSHALNRNLKRFKASKGKILGGGNSTYR 262

Query: 201 KGPLPL------RMSRHDRAVYNSVANRRKLSSSSDDGVLVNGIAVVSQDGEGDFLNITD 260
           + PL        +   +D+    +  N  +L  +S   +LV+   +V      +F  ITD
Sbjct: 263 E-PLETLIKGLRKTCDNDKDCRKTSRNLGELGETSGGSILVSKAVIVGPFKSDNFTTITD 322

Query: 261 AINAAPNNSLAANGYFLIYITAGVYQEYVSVPSKKKYLLMIGDGINQTIITGNRSVADGW 320
           AI AAPNN+   +GYF+IY   GVY+EY+ VP  KK L+++GDGIN+TIITGN +V DGW
Sbjct: 323 AIAAAPNNTRPEDGYFVIYAREGVYEEYIVVPINKKNLMLMGDGINKTIITGNHNVVDGW 382

Query: 321 TTFNSATFAVAAEGFMAVNITFQNTAGAIKGQAVALRSGADMCVFYSCSFEGYQDTLYTH 380
           TT+N ++FAV  E FMAV++TF+NTAG  K QAVALR+ A+   FY CSFEGYQDTLY H
Sbjct: 383 TTYNCSSFAVVGERFMAVDVTFRNTAGPEKHQAVALRNNAEGSSFYRCSFEGYQDTLYVH 442

Query: 381 SLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPGPRQANMITAQGRSDPNQNTGTSI 440
           SLRQF+RECDIYGTVDFIFGNAA +FQNCNIY R P  +Q N ITA GR DPNQNTG SI
Sbjct: 443 SLRQFYRECDIYGTVDFIFGNAAAIFQNCNIYARKPMAKQKNAITAHGRLDPNQNTGISI 502

Query: 441 HNCTIQATPDLAASSSHMNKTYLGRPWKQYSRTVYMQTFIDGFVNPKGWDPWSGEY-LST 500
            NCTI+A PDLAA       T+LGRPWK YSRTV+MQ++I   V P GW  W+G   L T
Sbjct: 503 INCTIKAAPDLAAEPKSA-MTFLGRPWKPYSRTVFMQSYISDIVQPVGWLEWNGTIGLDT 562

Query: 501 LYYGEYNNTGGGSDTKNRVIWPGYHVINNATDAANFTISNFLVGDVWLPPTGVPYTGGFV 546
           +YYGEY+N G G++T  RV W GY+++N A +A NFT+ NF +GD WLP T +P+ GG +
Sbjct: 563 IYYGEYSNFGPGANTNQRVQWLGYNLLNLA-EAMNFTVYNFTMGDTWLPQTDIPFYGGLL 616

BLAST of IVF0022449 vs. ExPASy Swiss-Prot
Match: Q9FF77 (Probable pectinesterase/pectinesterase inhibitor 47 OS=Arabidopsis thaliana OX=3702 GN=PME47 PE=2 SV=1)

HSP 1 Score: 442.2 bits (1136), Expect = 8.5e-123
Identity = 248/549 (45.17%), Postives = 330/549 (60.11%), Query Frame = 0

Query: 16  PTKAICKLTSNPSYCITVLK--QSRDGNIYDSGRFSIRRSLSKASRFLDLIEKHLQNSST 75
           P    CK T  P  C T+L   +S   + Y  G+F++++ L +A R   +I +  Q    
Sbjct: 83  PPNIACKSTPYPKLCRTILSAVKSSPSDPYHYGKFTMKQCLKQARRLSKVINRFAQRVEA 142

Query: 76  LPKSI----IGALKDCQYLAQLNMNFLSNSFRAVNGTDRKLTYSKADYIQSLLSAILTNI 135
            P +     + A+ DC  LA+L++ +L      +   +  +T +  D + SLL  ++TN 
Sbjct: 143 DPGTSTVEEVSAVADCGELAELSVEYLETVTEELKAAE-LMTAALVDRVTSLLGGVVTNQ 202

Query: 136 DTCLDGLNTVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPRWNRNRTLEHPGKKHL 195
            TCLDGL    SG +    +  PL + T+ YS+SL L +       NRN    + G K  
Sbjct: 203 QTCLDGLVDAKSGFATA--IGTPLGNLTRLYSVSLGLVSHA----LNRN-LKRYKGSKGK 262

Query: 196 QFRKGPLPLR----------MSRHDRAVYNSVANRR--KLSSSSDDGVLVNGIAVVSQDG 255
            F  G  P+R              D+      ANR   +L  +S   +LV     V    
Sbjct: 263 IFGGGNKPVREPLETLIKVLRKTCDKGKDCRKANRNLGELGETSGGSILVREAVTVGPYE 322

Query: 256 EGDFLNITDAINAAPNNSLAANGYFLIYITAGVYQEYVSVPSKKKYLLMIGDGINQTIIT 315
             +F  IT+A+ AAPN++    GYF+IY  AG+Y+EYV + +KK+ +++IGDGIN+TII+
Sbjct: 323 TDNFPTITEAVAAAPNHTFPEQGYFVIYARAGLYEEYVVISNKKRNIMLIGDGINKTIIS 382

Query: 316 GNRSVADGWTTFNSATFAVAAEGFMAVNITFQNTAGAIKGQAVALRSGADMCVFYSCSFE 375
           GN S  DGWTT+NS+TFAV  + F+AV++TF+NTAG  K QAVA+R+ AD   FY CSFE
Sbjct: 383 GNHSFIDGWTTYNSSTFAVVGDRFVAVDVTFRNTAGPEKHQAVAVRNNADGSTFYRCSFE 442

Query: 376 GYQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPGPRQANMITAQGRSD 435
           GYQDTLY HSLRQF+RECDIYGT+DFIFGNAA +FQNCNIY R P   Q N +TA GR+D
Sbjct: 443 GYQDTLYVHSLRQFYRECDIYGTIDFIFGNAAAIFQNCNIYARKPMANQKNAVTAHGRTD 502

Query: 436 PNQNTGTSIHNCTIQATPDLAASSSHMNKTYLGRPWKQYSRTVYMQTFIDGFVNPKGWDP 495
           PNQ TG SI NCTI A PDLAA       T+LGRPWK YSRTVY+Q++I   V P GW  
Sbjct: 503 PNQKTGISIINCTIGAAPDLAADPK-STMTFLGRPWKPYSRTVYIQSYISDVVQPVGWLE 562

Query: 496 WSGEY-LSTLYYGEYNNTGGGSDTKNRVIWPGYHVINNATDAANFTISNFLVGDVWLPPT 546
           W+G   L T+ YGEY+N G G+DT  RV W GY ++ N   A NFT+ NF +GD WLP T
Sbjct: 563 WNGTTGLDTISYGEYDNFGPGADTSKRVQWSGYSLL-NLVQAMNFTVYNFTLGDTWLPQT 621

BLAST of IVF0022449 vs. ExPASy TrEMBL
Match: A0A1S3CQK8 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103503665 PE=3 SV=1)

HSP 1 Score: 1108.2 bits (2865), Expect = 0.0e+00
Identity = 545/545 (100.00%), Postives = 545/545 (100.00%), Query Frame = 0

Query: 1   MVYLDSIYDSLTNIFPTKAICKLTSNPSYCITVLKQSRDGNIYDSGRFSIRRSLSKASRF 60
           MVYLDSIYDSLTNIFPTKAICKLTSNPSYCITVLKQSRDGNIYDSGRFSIRRSLSKASRF
Sbjct: 1   MVYLDSIYDSLTNIFPTKAICKLTSNPSYCITVLKQSRDGNIYDSGRFSIRRSLSKASRF 60

Query: 61  LDLIEKHLQNSSTLPKSIIGALKDCQYLAQLNMNFLSNSFRAVNGTDRKLTYSKADYIQS 120
           LDLIEKHLQNSSTLPKSIIGALKDCQYLAQLNMNFLSNSFRAVNGTDRKLTYSKADYIQS
Sbjct: 61  LDLIEKHLQNSSTLPKSIIGALKDCQYLAQLNMNFLSNSFRAVNGTDRKLTYSKADYIQS 120

Query: 121 LLSAILTNIDTCLDGLNTVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPRWNRNRT 180
           LLSAILTNIDTCLDGLNTVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPRWNRNRT
Sbjct: 121 LLSAILTNIDTCLDGLNTVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPRWNRNRT 180

Query: 181 LEHPGKKHLQFRKGPLPLRMSRHDRAVYNSVANRRKLSSSSDDGVLVNGIAVVSQDGEGD 240
           LEHPGKKHLQFRKGPLPLRMSRHDRAVYNSVANRRKLSSSSDDGVLVNGIAVVSQDGEGD
Sbjct: 181 LEHPGKKHLQFRKGPLPLRMSRHDRAVYNSVANRRKLSSSSDDGVLVNGIAVVSQDGEGD 240

Query: 241 FLNITDAINAAPNNSLAANGYFLIYITAGVYQEYVSVPSKKKYLLMIGDGINQTIITGNR 300
           FLNITDAINAAPNNSLAANGYFLIYITAGVYQEYVSVPSKKKYLLMIGDGINQTIITGNR
Sbjct: 241 FLNITDAINAAPNNSLAANGYFLIYITAGVYQEYVSVPSKKKYLLMIGDGINQTIITGNR 300

Query: 301 SVADGWTTFNSATFAVAAEGFMAVNITFQNTAGAIKGQAVALRSGADMCVFYSCSFEGYQ 360
           SVADGWTTFNSATFAVAAEGFMAVNITFQNTAGAIKGQAVALRSGADMCVFYSCSFEGYQ
Sbjct: 301 SVADGWTTFNSATFAVAAEGFMAVNITFQNTAGAIKGQAVALRSGADMCVFYSCSFEGYQ 360

Query: 361 DTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPGPRQANMITAQGRSDPNQ 420
           DTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPGPRQANMITAQGRSDPNQ
Sbjct: 361 DTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPGPRQANMITAQGRSDPNQ 420

Query: 421 NTGTSIHNCTIQATPDLAASSSHMNKTYLGRPWKQYSRTVYMQTFIDGFVNPKGWDPWSG 480
           NTGTSIHNCTIQATPDLAASSSHMNKTYLGRPWKQYSRTVYMQTFIDGFVNPKGWDPWSG
Sbjct: 421 NTGTSIHNCTIQATPDLAASSSHMNKTYLGRPWKQYSRTVYMQTFIDGFVNPKGWDPWSG 480

Query: 481 EYLSTLYYGEYNNTGGGSDTKNRVIWPGYHVINNATDAANFTISNFLVGDVWLPPTGVPY 540
           EYLSTLYYGEYNNTGGGSDTKNRVIWPGYHVINNATDAANFTISNFLVGDVWLPPTGVPY
Sbjct: 481 EYLSTLYYGEYNNTGGGSDTKNRVIWPGYHVINNATDAANFTISNFLVGDVWLPPTGVPY 540

Query: 541 TGGFV 546
           TGGFV
Sbjct: 541 TGGFV 545

BLAST of IVF0022449 vs. ExPASy TrEMBL
Match: A0A5A7TAP2 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G005740 PE=3 SV=1)

HSP 1 Score: 1092.8 bits (2825), Expect = 0.0e+00
Identity = 537/537 (100.00%), Postives = 537/537 (100.00%), Query Frame = 0

Query: 9   DSLTNIFPTKAICKLTSNPSYCITVLKQSRDGNIYDSGRFSIRRSLSKASRFLDLIEKHL 68
           DSLTNIFPTKAICKLTSNPSYCITVLKQSRDGNIYDSGRFSIRRSLSKASRFLDLIEKHL
Sbjct: 26  DSLTNIFPTKAICKLTSNPSYCITVLKQSRDGNIYDSGRFSIRRSLSKASRFLDLIEKHL 85

Query: 69  QNSSTLPKSIIGALKDCQYLAQLNMNFLSNSFRAVNGTDRKLTYSKADYIQSLLSAILTN 128
           QNSSTLPKSIIGALKDCQYLAQLNMNFLSNSFRAVNGTDRKLTYSKADYIQSLLSAILTN
Sbjct: 86  QNSSTLPKSIIGALKDCQYLAQLNMNFLSNSFRAVNGTDRKLTYSKADYIQSLLSAILTN 145

Query: 129 IDTCLDGLNTVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPRWNRNRTLEHPGKKH 188
           IDTCLDGLNTVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPRWNRNRTLEHPGKKH
Sbjct: 146 IDTCLDGLNTVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPRWNRNRTLEHPGKKH 205

Query: 189 LQFRKGPLPLRMSRHDRAVYNSVANRRKLSSSSDDGVLVNGIAVVSQDGEGDFLNITDAI 248
           LQFRKGPLPLRMSRHDRAVYNSVANRRKLSSSSDDGVLVNGIAVVSQDGEGDFLNITDAI
Sbjct: 206 LQFRKGPLPLRMSRHDRAVYNSVANRRKLSSSSDDGVLVNGIAVVSQDGEGDFLNITDAI 265

Query: 249 NAAPNNSLAANGYFLIYITAGVYQEYVSVPSKKKYLLMIGDGINQTIITGNRSVADGWTT 308
           NAAPNNSLAANGYFLIYITAGVYQEYVSVPSKKKYLLMIGDGINQTIITGNRSVADGWTT
Sbjct: 266 NAAPNNSLAANGYFLIYITAGVYQEYVSVPSKKKYLLMIGDGINQTIITGNRSVADGWTT 325

Query: 309 FNSATFAVAAEGFMAVNITFQNTAGAIKGQAVALRSGADMCVFYSCSFEGYQDTLYTHSL 368
           FNSATFAVAAEGFMAVNITFQNTAGAIKGQAVALRSGADMCVFYSCSFEGYQDTLYTHSL
Sbjct: 326 FNSATFAVAAEGFMAVNITFQNTAGAIKGQAVALRSGADMCVFYSCSFEGYQDTLYTHSL 385

Query: 369 RQFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPGPRQANMITAQGRSDPNQNTGTSIHN 428
           RQFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPGPRQANMITAQGRSDPNQNTGTSIHN
Sbjct: 386 RQFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPGPRQANMITAQGRSDPNQNTGTSIHN 445

Query: 429 CTIQATPDLAASSSHMNKTYLGRPWKQYSRTVYMQTFIDGFVNPKGWDPWSGEYLSTLYY 488
           CTIQATPDLAASSSHMNKTYLGRPWKQYSRTVYMQTFIDGFVNPKGWDPWSGEYLSTLYY
Sbjct: 446 CTIQATPDLAASSSHMNKTYLGRPWKQYSRTVYMQTFIDGFVNPKGWDPWSGEYLSTLYY 505

Query: 489 GEYNNTGGGSDTKNRVIWPGYHVINNATDAANFTISNFLVGDVWLPPTGVPYTGGFV 546
           GEYNNTGGGSDTKNRVIWPGYHVINNATDAANFTISNFLVGDVWLPPTGVPYTGGFV
Sbjct: 506 GEYNNTGGGSDTKNRVIWPGYHVINNATDAANFTISNFLVGDVWLPPTGVPYTGGFV 562

BLAST of IVF0022449 vs. ExPASy TrEMBL
Match: A0A1S4E6G9 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103503665 PE=3 SV=1)

HSP 1 Score: 1092.8 bits (2825), Expect = 0.0e+00
Identity = 537/537 (100.00%), Postives = 537/537 (100.00%), Query Frame = 0

Query: 9   DSLTNIFPTKAICKLTSNPSYCITVLKQSRDGNIYDSGRFSIRRSLSKASRFLDLIEKHL 68
           DSLTNIFPTKAICKLTSNPSYCITVLKQSRDGNIYDSGRFSIRRSLSKASRFLDLIEKHL
Sbjct: 26  DSLTNIFPTKAICKLTSNPSYCITVLKQSRDGNIYDSGRFSIRRSLSKASRFLDLIEKHL 85

Query: 69  QNSSTLPKSIIGALKDCQYLAQLNMNFLSNSFRAVNGTDRKLTYSKADYIQSLLSAILTN 128
           QNSSTLPKSIIGALKDCQYLAQLNMNFLSNSFRAVNGTDRKLTYSKADYIQSLLSAILTN
Sbjct: 86  QNSSTLPKSIIGALKDCQYLAQLNMNFLSNSFRAVNGTDRKLTYSKADYIQSLLSAILTN 145

Query: 129 IDTCLDGLNTVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPRWNRNRTLEHPGKKH 188
           IDTCLDGLNTVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPRWNRNRTLEHPGKKH
Sbjct: 146 IDTCLDGLNTVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPRWNRNRTLEHPGKKH 205

Query: 189 LQFRKGPLPLRMSRHDRAVYNSVANRRKLSSSSDDGVLVNGIAVVSQDGEGDFLNITDAI 248
           LQFRKGPLPLRMSRHDRAVYNSVANRRKLSSSSDDGVLVNGIAVVSQDGEGDFLNITDAI
Sbjct: 206 LQFRKGPLPLRMSRHDRAVYNSVANRRKLSSSSDDGVLVNGIAVVSQDGEGDFLNITDAI 265

Query: 249 NAAPNNSLAANGYFLIYITAGVYQEYVSVPSKKKYLLMIGDGINQTIITGNRSVADGWTT 308
           NAAPNNSLAANGYFLIYITAGVYQEYVSVPSKKKYLLMIGDGINQTIITGNRSVADGWTT
Sbjct: 266 NAAPNNSLAANGYFLIYITAGVYQEYVSVPSKKKYLLMIGDGINQTIITGNRSVADGWTT 325

Query: 309 FNSATFAVAAEGFMAVNITFQNTAGAIKGQAVALRSGADMCVFYSCSFEGYQDTLYTHSL 368
           FNSATFAVAAEGFMAVNITFQNTAGAIKGQAVALRSGADMCVFYSCSFEGYQDTLYTHSL
Sbjct: 326 FNSATFAVAAEGFMAVNITFQNTAGAIKGQAVALRSGADMCVFYSCSFEGYQDTLYTHSL 385

Query: 369 RQFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPGPRQANMITAQGRSDPNQNTGTSIHN 428
           RQFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPGPRQANMITAQGRSDPNQNTGTSIHN
Sbjct: 386 RQFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPGPRQANMITAQGRSDPNQNTGTSIHN 445

Query: 429 CTIQATPDLAASSSHMNKTYLGRPWKQYSRTVYMQTFIDGFVNPKGWDPWSGEYLSTLYY 488
           CTIQATPDLAASSSHMNKTYLGRPWKQYSRTVYMQTFIDGFVNPKGWDPWSGEYLSTLYY
Sbjct: 446 CTIQATPDLAASSSHMNKTYLGRPWKQYSRTVYMQTFIDGFVNPKGWDPWSGEYLSTLYY 505

Query: 489 GEYNNTGGGSDTKNRVIWPGYHVINNATDAANFTISNFLVGDVWLPPTGVPYTGGFV 546
           GEYNNTGGGSDTKNRVIWPGYHVINNATDAANFTISNFLVGDVWLPPTGVPYTGGFV
Sbjct: 506 GEYNNTGGGSDTKNRVIWPGYHVINNATDAANFTISNFLVGDVWLPPTGVPYTGGFV 562

BLAST of IVF0022449 vs. ExPASy TrEMBL
Match: A0A0A0LHM0 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_3G890080 PE=3 SV=1)

HSP 1 Score: 1047.0 bits (2706), Expect = 2.8e-302
Identity = 514/537 (95.72%), Postives = 524/537 (97.58%), Query Frame = 0

Query: 9   DSLTNIFPTKAICKLTSNPSYCITVLKQSRDGNIYDSGRFSIRRSLSKASRFLDLIEKHL 68
           DSLTN FPTKAICKLTSNPSYCITVLKQSRDGNIYDSGRFSIRRSLSKA+RFLDLIEKHL
Sbjct: 29  DSLTNFFPTKAICKLTSNPSYCITVLKQSRDGNIYDSGRFSIRRSLSKATRFLDLIEKHL 88

Query: 69  QNSSTLPKSIIGALKDCQYLAQLNMNFLSNSFRAVNGTDRKLTYSKADYIQSLLSAILTN 128
           QNSSTLP SIIGALKDCQYLAQLNMNFLSNSFRAVNGTDRKLTYSKADYIQSLLSAILTN
Sbjct: 89  QNSSTLPNSIIGALKDCQYLAQLNMNFLSNSFRAVNGTDRKLTYSKADYIQSLLSAILTN 148

Query: 129 IDTCLDGLNTVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPRWNRNRTLEHPGKKH 188
           IDTCLDGLNTVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPR NRNRTLEHPGKKH
Sbjct: 149 IDTCLDGLNTVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPRRNRNRTLEHPGKKH 208

Query: 189 LQFRKGPLPLRMSRHDRAVYNSVANRRKLSSSSDDGVLVNGIAVVSQDGEGDFLNITDAI 248
           LQFRKGPLPLRMSRHDRAVYNSVANRRKLSSSSDDGVLVNG+ VVSQDG+GDFLNITDAI
Sbjct: 209 LQFRKGPLPLRMSRHDRAVYNSVANRRKLSSSSDDGVLVNGVVVVSQDGQGDFLNITDAI 268

Query: 249 NAAPNNSLAANGYFLIYITAGVYQEYVSVPSKKKYLLMIGDGINQTIITGNRSVADGWTT 308
           NAAPNNSLA++GYFLIYITAGVYQEYVSVPSKKKYLLMIGDGINQTIITGNRSVADGWTT
Sbjct: 269 NAAPNNSLASDGYFLIYITAGVYQEYVSVPSKKKYLLMIGDGINQTIITGNRSVADGWTT 328

Query: 309 FNSATFAVAAEGFMAVNITFQNTAGAIKGQAVALRSGADMCVFYSCSFEGYQDTLYTHSL 368
           FNSATFAVAAEGFMAVNIT QNTAGAIKGQAVALRSGADMCVFYSCSFEG+QDTLYTHSL
Sbjct: 329 FNSATFAVAAEGFMAVNITIQNTAGAIKGQAVALRSGADMCVFYSCSFEGFQDTLYTHSL 388

Query: 369 RQFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPGPRQANMITAQGRSDPNQNTGTSIHN 428
           RQFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLP P QANMITAQGRSDPNQNTGTSIHN
Sbjct: 389 RQFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPRPGQANMITAQGRSDPNQNTGTSIHN 448

Query: 429 CTIQATPDLAASSSHMNKTYLGRPWKQYSRTVYMQTFIDGFVNPKGWDPWSGEYLSTLYY 488
           CTI+ATP+LAASSS+MNKTYLGRPWKQYSRTVYMQTFIDGFVNPKGWDPW+GEYLSTLYY
Sbjct: 449 CTIRATPELAASSSYMNKTYLGRPWKQYSRTVYMQTFIDGFVNPKGWDPWTGEYLSTLYY 508

Query: 489 GEYNNTGGGSDTKNRVIWPGYHVINNATDAANFTISNFLVGDVWLPPTGVPYTGGFV 546
           GEYNNTGGGSDTKNRV W GYHVINN TDAANFTISNFLVGD WLPPT VPY GGFV
Sbjct: 509 GEYNNTGGGSDTKNRVTWAGYHVINNVTDAANFTISNFLVGDAWLPPTWVPYMGGFV 565

BLAST of IVF0022449 vs. ExPASy TrEMBL
Match: A0A6J1J323 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111480894 PE=3 SV=1)

HSP 1 Score: 971.1 bits (2509), Expect = 2.0e-279
Identity = 473/536 (88.25%), Postives = 506/536 (94.40%), Query Frame = 0

Query: 10  SLTNIFPTKAICKLTSNPSYCITVLKQSRDGNIYDSGRFSIRRSLSKASRFLDLIEKHLQ 69
           SL ++F  KAICK TSNPSYCI+VL++S  GN+Y+SGRFS+RRSL+KA+ FL+LIEKHLQ
Sbjct: 10  SLNDLFLAKAICKFTSNPSYCISVLQKS-SGNVYESGRFSMRRSLAKATTFLNLIEKHLQ 69

Query: 70  NSSTLPKSIIGALKDCQYLAQLNMNFLSNSFRAVNGTDRKLTYSKADYIQSLLSAILTNI 129
           NSSTLPKS+ G LKDCQYLAQLN+NFLSNSFRAVNGTD+KLTYSKADYIQSLLSAILTNI
Sbjct: 70  NSSTLPKSVTGGLKDCQYLAQLNINFLSNSFRAVNGTDKKLTYSKADYIQSLLSAILTNI 129

Query: 130 DTCLDGLNTVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPRWNRNRTLEHPGKKHL 189
           DTCLDGLNTVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPR NRNRT +HP  KHL
Sbjct: 130 DTCLDGLNTVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPR-NRNRTRDHPVTKHL 189

Query: 190 QFRKGPLPLRMSRHDRAVYNSVANRRKLSSSSDDGVLVNGIAVVSQDGEGDFLNITDAIN 249
           +FRKGPLPLRMS+HDRAVYNSVANRR+LSSSSDDG+LVNGI VVSQDG G+FL IT+A+N
Sbjct: 190 RFRKGPLPLRMSKHDRAVYNSVANRRRLSSSSDDGILVNGIVVVSQDGTGNFLTITEAVN 249

Query: 250 AAPNNSLAANGYFLIYITAGVYQEYVSVPSKKKYLLMIGDGINQTIITGNRSVADGWTTF 309
           AAPNNSLA NGYFLIYI AGVYQEYVS+PSKKKYLLMIGDGINQTIITGN+SVADGWTTF
Sbjct: 250 AAPNNSLAVNGYFLIYIAAGVYQEYVSIPSKKKYLLMIGDGINQTIITGNQSVADGWTTF 309

Query: 310 NSATFAVAAEGFMAVNITFQNTAGAIKGQAVALRSGADMCVFYSCSFEGYQDTLYTHSLR 369
           NSATFAVAAEGFMAVNITFQNTAGAIKGQAVA+RSGADM +FYSCSFEGYQDTLYTHSLR
Sbjct: 310 NSATFAVAAEGFMAVNITFQNTAGAIKGQAVAMRSGADMSIFYSCSFEGYQDTLYTHSLR 369

Query: 370 QFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPGPRQANMITAQGRSDPNQNTGTSIHNC 429
           QFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLP   Q NMITAQGRSDPNQNTGTSIHNC
Sbjct: 370 QFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPRSGQTNMITAQGRSDPNQNTGTSIHNC 429

Query: 430 TIQATPDLAASSSHMNKTYLGRPWKQYSRTVYMQTFIDGFVNPKGWDPWSGEYLSTLYYG 489
           TI+AT DLAAS S+MNKTYLGRPWKQYSRTVYMQTFIDGFVNP GWDPWSGEYLSTLYY 
Sbjct: 430 TIRATSDLAASKSYMNKTYLGRPWKQYSRTVYMQTFIDGFVNPAGWDPWSGEYLSTLYYA 489

Query: 490 EYNNTGGGSDTKNRVIWPGYHVINNATDAANFTISNFLVGDVWLPPTGVPYTGGFV 546
           EYNNTG GS+TKNRV WPGYHVI+NATDA+NFT+SNFLVGD WLPPTGVPYTGGF+
Sbjct: 490 EYNNTGAGSNTKNRVTWPGYHVISNATDASNFTVSNFLVGDAWLPPTGVPYTGGFI 543

BLAST of IVF0022449 vs. NCBI nr
Match: XP_008466170.1 (PREDICTED: probable pectinesterase/pectinesterase inhibitor 20 isoform X2 [Cucumis melo] >XP_008466171.1 PREDICTED: probable pectinesterase/pectinesterase inhibitor 20 isoform X2 [Cucumis melo])

HSP 1 Score: 1107 bits (2864), Expect = 0.0
Identity = 545/545 (100.00%), Postives = 545/545 (100.00%), Query Frame = 0

Query: 1   MVYLDSIYDSLTNIFPTKAICKLTSNPSYCITVLKQSRDGNIYDSGRFSIRRSLSKASRF 60
           MVYLDSIYDSLTNIFPTKAICKLTSNPSYCITVLKQSRDGNIYDSGRFSIRRSLSKASRF
Sbjct: 1   MVYLDSIYDSLTNIFPTKAICKLTSNPSYCITVLKQSRDGNIYDSGRFSIRRSLSKASRF 60

Query: 61  LDLIEKHLQNSSTLPKSIIGALKDCQYLAQLNMNFLSNSFRAVNGTDRKLTYSKADYIQS 120
           LDLIEKHLQNSSTLPKSIIGALKDCQYLAQLNMNFLSNSFRAVNGTDRKLTYSKADYIQS
Sbjct: 61  LDLIEKHLQNSSTLPKSIIGALKDCQYLAQLNMNFLSNSFRAVNGTDRKLTYSKADYIQS 120

Query: 121 LLSAILTNIDTCLDGLNTVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPRWNRNRT 180
           LLSAILTNIDTCLDGLNTVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPRWNRNRT
Sbjct: 121 LLSAILTNIDTCLDGLNTVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPRWNRNRT 180

Query: 181 LEHPGKKHLQFRKGPLPLRMSRHDRAVYNSVANRRKLSSSSDDGVLVNGIAVVSQDGEGD 240
           LEHPGKKHLQFRKGPLPLRMSRHDRAVYNSVANRRKLSSSSDDGVLVNGIAVVSQDGEGD
Sbjct: 181 LEHPGKKHLQFRKGPLPLRMSRHDRAVYNSVANRRKLSSSSDDGVLVNGIAVVSQDGEGD 240

Query: 241 FLNITDAINAAPNNSLAANGYFLIYITAGVYQEYVSVPSKKKYLLMIGDGINQTIITGNR 300
           FLNITDAINAAPNNSLAANGYFLIYITAGVYQEYVSVPSKKKYLLMIGDGINQTIITGNR
Sbjct: 241 FLNITDAINAAPNNSLAANGYFLIYITAGVYQEYVSVPSKKKYLLMIGDGINQTIITGNR 300

Query: 301 SVADGWTTFNSATFAVAAEGFMAVNITFQNTAGAIKGQAVALRSGADMCVFYSCSFEGYQ 360
           SVADGWTTFNSATFAVAAEGFMAVNITFQNTAGAIKGQAVALRSGADMCVFYSCSFEGYQ
Sbjct: 301 SVADGWTTFNSATFAVAAEGFMAVNITFQNTAGAIKGQAVALRSGADMCVFYSCSFEGYQ 360

Query: 361 DTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPGPRQANMITAQGRSDPNQ 420
           DTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPGPRQANMITAQGRSDPNQ
Sbjct: 361 DTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPGPRQANMITAQGRSDPNQ 420

Query: 421 NTGTSIHNCTIQATPDLAASSSHMNKTYLGRPWKQYSRTVYMQTFIDGFVNPKGWDPWSG 480
           NTGTSIHNCTIQATPDLAASSSHMNKTYLGRPWKQYSRTVYMQTFIDGFVNPKGWDPWSG
Sbjct: 421 NTGTSIHNCTIQATPDLAASSSHMNKTYLGRPWKQYSRTVYMQTFIDGFVNPKGWDPWSG 480

Query: 481 EYLSTLYYGEYNNTGGGSDTKNRVIWPGYHVINNATDAANFTISNFLVGDVWLPPTGVPY 540
           EYLSTLYYGEYNNTGGGSDTKNRVIWPGYHVINNATDAANFTISNFLVGDVWLPPTGVPY
Sbjct: 481 EYLSTLYYGEYNNTGGGSDTKNRVIWPGYHVINNATDAANFTISNFLVGDVWLPPTGVPY 540

Query: 541 TGGFV 545
           TGGFV
Sbjct: 541 TGGFV 545

BLAST of IVF0022449 vs. NCBI nr
Match: XP_016903565.1 (PREDICTED: probable pectinesterase/pectinesterase inhibitor 20 isoform X1 [Cucumis melo] >KAA0038671.1 putative pectinesterase/pectinesterase inhibitor 20 isoform X1 [Cucumis melo var. makuwa] >TYK31280.1 putative pectinesterase/pectinesterase inhibitor 20 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 1092 bits (2823), Expect = 0.0
Identity = 537/537 (100.00%), Postives = 537/537 (100.00%), Query Frame = 0

Query: 9   DSLTNIFPTKAICKLTSNPSYCITVLKQSRDGNIYDSGRFSIRRSLSKASRFLDLIEKHL 68
           DSLTNIFPTKAICKLTSNPSYCITVLKQSRDGNIYDSGRFSIRRSLSKASRFLDLIEKHL
Sbjct: 26  DSLTNIFPTKAICKLTSNPSYCITVLKQSRDGNIYDSGRFSIRRSLSKASRFLDLIEKHL 85

Query: 69  QNSSTLPKSIIGALKDCQYLAQLNMNFLSNSFRAVNGTDRKLTYSKADYIQSLLSAILTN 128
           QNSSTLPKSIIGALKDCQYLAQLNMNFLSNSFRAVNGTDRKLTYSKADYIQSLLSAILTN
Sbjct: 86  QNSSTLPKSIIGALKDCQYLAQLNMNFLSNSFRAVNGTDRKLTYSKADYIQSLLSAILTN 145

Query: 129 IDTCLDGLNTVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPRWNRNRTLEHPGKKH 188
           IDTCLDGLNTVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPRWNRNRTLEHPGKKH
Sbjct: 146 IDTCLDGLNTVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPRWNRNRTLEHPGKKH 205

Query: 189 LQFRKGPLPLRMSRHDRAVYNSVANRRKLSSSSDDGVLVNGIAVVSQDGEGDFLNITDAI 248
           LQFRKGPLPLRMSRHDRAVYNSVANRRKLSSSSDDGVLVNGIAVVSQDGEGDFLNITDAI
Sbjct: 206 LQFRKGPLPLRMSRHDRAVYNSVANRRKLSSSSDDGVLVNGIAVVSQDGEGDFLNITDAI 265

Query: 249 NAAPNNSLAANGYFLIYITAGVYQEYVSVPSKKKYLLMIGDGINQTIITGNRSVADGWTT 308
           NAAPNNSLAANGYFLIYITAGVYQEYVSVPSKKKYLLMIGDGINQTIITGNRSVADGWTT
Sbjct: 266 NAAPNNSLAANGYFLIYITAGVYQEYVSVPSKKKYLLMIGDGINQTIITGNRSVADGWTT 325

Query: 309 FNSATFAVAAEGFMAVNITFQNTAGAIKGQAVALRSGADMCVFYSCSFEGYQDTLYTHSL 368
           FNSATFAVAAEGFMAVNITFQNTAGAIKGQAVALRSGADMCVFYSCSFEGYQDTLYTHSL
Sbjct: 326 FNSATFAVAAEGFMAVNITFQNTAGAIKGQAVALRSGADMCVFYSCSFEGYQDTLYTHSL 385

Query: 369 RQFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPGPRQANMITAQGRSDPNQNTGTSIHN 428
           RQFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPGPRQANMITAQGRSDPNQNTGTSIHN
Sbjct: 386 RQFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPGPRQANMITAQGRSDPNQNTGTSIHN 445

Query: 429 CTIQATPDLAASSSHMNKTYLGRPWKQYSRTVYMQTFIDGFVNPKGWDPWSGEYLSTLYY 488
           CTIQATPDLAASSSHMNKTYLGRPWKQYSRTVYMQTFIDGFVNPKGWDPWSGEYLSTLYY
Sbjct: 446 CTIQATPDLAASSSHMNKTYLGRPWKQYSRTVYMQTFIDGFVNPKGWDPWSGEYLSTLYY 505

Query: 489 GEYNNTGGGSDTKNRVIWPGYHVINNATDAANFTISNFLVGDVWLPPTGVPYTGGFV 545
           GEYNNTGGGSDTKNRVIWPGYHVINNATDAANFTISNFLVGDVWLPPTGVPYTGGFV
Sbjct: 506 GEYNNTGGGSDTKNRVIWPGYHVINNATDAANFTISNFLVGDVWLPPTGVPYTGGFV 562

BLAST of IVF0022449 vs. NCBI nr
Match: XP_031738568.1 (probable pectinesterase/pectinesterase inhibitor 20 isoform X1 [Cucumis sativus] >XP_031738569.1 probable pectinesterase/pectinesterase inhibitor 20 isoform X1 [Cucumis sativus] >XP_031738570.1 probable pectinesterase/pectinesterase inhibitor 20 isoform X1 [Cucumis sativus] >KGN60252.1 hypothetical protein Csa_001115 [Cucumis sativus])

HSP 1 Score: 1046 bits (2704), Expect = 0.0
Identity = 514/537 (95.72%), Postives = 524/537 (97.58%), Query Frame = 0

Query: 9   DSLTNIFPTKAICKLTSNPSYCITVLKQSRDGNIYDSGRFSIRRSLSKASRFLDLIEKHL 68
           DSLTN FPTKAICKLTSNPSYCITVLKQSRDGNIYDSGRFSIRRSLSKA+RFLDLIEKHL
Sbjct: 29  DSLTNFFPTKAICKLTSNPSYCITVLKQSRDGNIYDSGRFSIRRSLSKATRFLDLIEKHL 88

Query: 69  QNSSTLPKSIIGALKDCQYLAQLNMNFLSNSFRAVNGTDRKLTYSKADYIQSLLSAILTN 128
           QNSSTLP SIIGALKDCQYLAQLNMNFLSNSFRAVNGTDRKLTYSKADYIQSLLSAILTN
Sbjct: 89  QNSSTLPNSIIGALKDCQYLAQLNMNFLSNSFRAVNGTDRKLTYSKADYIQSLLSAILTN 148

Query: 129 IDTCLDGLNTVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPRWNRNRTLEHPGKKH 188
           IDTCLDGLNTVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPR NRNRTLEHPGKKH
Sbjct: 149 IDTCLDGLNTVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPRRNRNRTLEHPGKKH 208

Query: 189 LQFRKGPLPLRMSRHDRAVYNSVANRRKLSSSSDDGVLVNGIAVVSQDGEGDFLNITDAI 248
           LQFRKGPLPLRMSRHDRAVYNSVANRRKLSSSSDDGVLVNG+ VVSQDG+GDFLNITDAI
Sbjct: 209 LQFRKGPLPLRMSRHDRAVYNSVANRRKLSSSSDDGVLVNGVVVVSQDGQGDFLNITDAI 268

Query: 249 NAAPNNSLAANGYFLIYITAGVYQEYVSVPSKKKYLLMIGDGINQTIITGNRSVADGWTT 308
           NAAPNNSLA++GYFLIYITAGVYQEYVSVPSKKKYLLMIGDGINQTIITGNRSVADGWTT
Sbjct: 269 NAAPNNSLASDGYFLIYITAGVYQEYVSVPSKKKYLLMIGDGINQTIITGNRSVADGWTT 328

Query: 309 FNSATFAVAAEGFMAVNITFQNTAGAIKGQAVALRSGADMCVFYSCSFEGYQDTLYTHSL 368
           FNSATFAVAAEGFMAVNIT QNTAGAIKGQAVALRSGADMCVFYSCSFEG+QDTLYTHSL
Sbjct: 329 FNSATFAVAAEGFMAVNITIQNTAGAIKGQAVALRSGADMCVFYSCSFEGFQDTLYTHSL 388

Query: 369 RQFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPGPRQANMITAQGRSDPNQNTGTSIHN 428
           RQFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLP P QANMITAQGRSDPNQNTGTSIHN
Sbjct: 389 RQFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPRPGQANMITAQGRSDPNQNTGTSIHN 448

Query: 429 CTIQATPDLAASSSHMNKTYLGRPWKQYSRTVYMQTFIDGFVNPKGWDPWSGEYLSTLYY 488
           CTI+ATP+LAASSS+MNKTYLGRPWKQYSRTVYMQTFIDGFVNPKGWDPW+GEYLSTLYY
Sbjct: 449 CTIRATPELAASSSYMNKTYLGRPWKQYSRTVYMQTFIDGFVNPKGWDPWTGEYLSTLYY 508

Query: 489 GEYNNTGGGSDTKNRVIWPGYHVINNATDAANFTISNFLVGDVWLPPTGVPYTGGFV 545
           GEYNNTGGGSDTKNRV W GYHVINN TDAANFTISNFLVGD WLPPT VPY GGFV
Sbjct: 509 GEYNNTGGGSDTKNRVTWAGYHVINNVTDAANFTISNFLVGDAWLPPTWVPYMGGFV 565

BLAST of IVF0022449 vs. NCBI nr
Match: XP_038898430.1 (probable pectinesterase/pectinesterase inhibitor 20 isoform X1 [Benincasa hispida] >XP_038898431.1 probable pectinesterase/pectinesterase inhibitor 20 isoform X1 [Benincasa hispida] >XP_038898432.1 probable pectinesterase/pectinesterase inhibitor 20 isoform X1 [Benincasa hispida])

HSP 1 Score: 1011 bits (2614), Expect = 0.0
Identity = 498/538 (92.57%), Postives = 514/538 (95.54%), Query Frame = 0

Query: 8   YDSLTNIFPTKAICKLTSNPSYCITVLKQSRDGNIYDSGRFSIRRSLSKASRFLDLIEKH 67
           YDSLTN  P+KAICK TSNPSYCITVLK+S  GNIYDSGRFSIRRSLSKASRF DLIEKH
Sbjct: 28  YDSLTNFPPSKAICKFTSNPSYCITVLKKSTGGNIYDSGRFSIRRSLSKASRFFDLIEKH 87

Query: 68  LQNSSTLPKSIIGALKDCQYLAQLNMNFLSNSFRAVNGTDRKLTYSKADYIQSLLSAILT 127
           LQNSSTLPKSI GAL DCQYLAQLN+NFLSNSFRAVNGTD+KLTYSKADYIQSLLSAILT
Sbjct: 88  LQNSSTLPKSITGALTDCQYLAQLNINFLSNSFRAVNGTDKKLTYSKADYIQSLLSAILT 147

Query: 128 NIDTCLDGLNTVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPRWNRNRTLEHPGKK 187
           NIDTCLDGL TVA+GSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPR NRNRTL+HPGKK
Sbjct: 148 NIDTCLDGLKTVAAGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPRRNRNRTLDHPGKK 207

Query: 188 HLQFRKGPLPLRMSRHDRAVYNSVANRRKLSSSSDDGVLVNGIAVVSQDGEGDFLNITDA 247
           HLQFRKGPLPLRMSR DRAVY+SVANRRKLSSSSDDGVLVNGIAVVSQDG+GDFLNITDA
Sbjct: 208 HLQFRKGPLPLRMSRRDRAVYDSVANRRKLSSSSDDGVLVNGIAVVSQDGKGDFLNITDA 267

Query: 248 INAAPNNSLAANGYFLIYITAGVYQEYVSVPSKKKYLLMIGDGINQTIITGNRSVADGWT 307
           INAAPNNSLAANGYFLIYI+AGVYQEYVS+PSKKKYLLMIGDGINQTIITGN SV DGWT
Sbjct: 268 INAAPNNSLAANGYFLIYISAGVYQEYVSIPSKKKYLLMIGDGINQTIITGNHSVVDGWT 327

Query: 308 TFNSATFAVAAEGFMAVNITFQNTAGAIKGQAVALRSGADMCVFYSCSFEGYQDTLYTHS 367
           TFNSATFAVAAEGFMAVNITFQNTAGAIKGQAVALRSGADMCVFYSCSFEGYQDTLYTHS
Sbjct: 328 TFNSATFAVAAEGFMAVNITFQNTAGAIKGQAVALRSGADMCVFYSCSFEGYQDTLYTHS 387

Query: 368 LRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPGPRQANMITAQGRSDPNQNTGTSIH 427
           LRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLP P QANMITAQGRSDPNQNTGTSIH
Sbjct: 388 LRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPRPGQANMITAQGRSDPNQNTGTSIH 447

Query: 428 NCTIQATPDLAASSSHMNKTYLGRPWKQYSRTVYMQTFIDGFVNPKGWDPWSGEYLSTLY 487
           NC I+AT DLAASSS+MNKTYLGRPWKQYSRTVYMQTFIDGFVNP GWDPW+GEYLSTLY
Sbjct: 448 NCAIRATSDLAASSSYMNKTYLGRPWKQYSRTVYMQTFIDGFVNPSGWDPWTGEYLSTLY 507

Query: 488 YGEYNNTGGGSDTKNRVIWPGYHVINNATDAANFTISNFLVGDVWLPPTGVPYTGGFV 545
           YGEYNNTG GS+TKNRV W GYHVINNATDAANFT+SNFLVGDVWLP T VPYTG F+
Sbjct: 508 YGEYNNTGDGSNTKNRVNWAGYHVINNATDAANFTVSNFLVGDVWLPQTCVPYTGAFI 565

BLAST of IVF0022449 vs. NCBI nr
Match: XP_023524144.1 (probable pectinesterase/pectinesterase inhibitor 20 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023524145.1 probable pectinesterase/pectinesterase inhibitor 20 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023524146.1 probable pectinesterase/pectinesterase inhibitor 20 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 974 bits (2518), Expect = 0.0
Identity = 474/536 (88.43%), Postives = 506/536 (94.40%), Query Frame = 0

Query: 10  SLTNIFPTKAICKLTSNPSYCITVLKQSRDGNIYDSGRFSIRRSLSKASRFLDLIEKHLQ 69
           SL ++FP KAICK TSNPSYCI+VL++S  GN+Y+SGRFS+RRSLSKAS FL+LIEKHLQ
Sbjct: 10  SLNDLFPAKAICKFTSNPSYCISVLQKS-SGNVYESGRFSMRRSLSKASAFLNLIEKHLQ 69

Query: 70  NSSTLPKSIIGALKDCQYLAQLNMNFLSNSFRAVNGTDRKLTYSKADYIQSLLSAILTNI 129
           NSSTLPKS+ G LKDCQYLAQLN+NFLSNSFRAVNGTD+KLTYSKADYIQSLLSAILTNI
Sbjct: 70  NSSTLPKSVTGGLKDCQYLAQLNINFLSNSFRAVNGTDKKLTYSKADYIQSLLSAILTNI 129

Query: 130 DTCLDGLNTVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPRWNRNRTLEHPGKKHL 189
           DTCLDGLNTVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPR NRNRT +HP  KHL
Sbjct: 130 DTCLDGLNTVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPR-NRNRTRDHPVTKHL 189

Query: 190 QFRKGPLPLRMSRHDRAVYNSVANRRKLSSSSDDGVLVNGIAVVSQDGEGDFLNITDAIN 249
           +FRKGPLPLRMS+HDR VYNSVANRR+LSSSSDDG+LVNGI VVSQDG G+FL IT+A+N
Sbjct: 190 RFRKGPLPLRMSKHDRVVYNSVANRRRLSSSSDDGILVNGIVVVSQDGTGNFLTITEAVN 249

Query: 250 AAPNNSLAANGYFLIYITAGVYQEYVSVPSKKKYLLMIGDGINQTIITGNRSVADGWTTF 309
           AAPNNSLA NGYFLIYI AGVYQEYVS+PSKKKYLLMIGDGINQTIITGN+SVADGWTTF
Sbjct: 250 AAPNNSLAVNGYFLIYIAAGVYQEYVSIPSKKKYLLMIGDGINQTIITGNQSVADGWTTF 309

Query: 310 NSATFAVAAEGFMAVNITFQNTAGAIKGQAVALRSGADMCVFYSCSFEGYQDTLYTHSLR 369
           NSATFAVAAEGFMAVNITFQNTAGAIKGQAVA+RSGADM +FYSCSFEGYQDTLYTHSLR
Sbjct: 310 NSATFAVAAEGFMAVNITFQNTAGAIKGQAVAMRSGADMSIFYSCSFEGYQDTLYTHSLR 369

Query: 370 QFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPGPRQANMITAQGRSDPNQNTGTSIHNC 429
           QFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLP   Q NMITAQGRSDPNQNTGTSIHNC
Sbjct: 370 QFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPRSGQTNMITAQGRSDPNQNTGTSIHNC 429

Query: 430 TIQATPDLAASSSHMNKTYLGRPWKQYSRTVYMQTFIDGFVNPKGWDPWSGEYLSTLYYG 489
           TI+AT DLAAS S+MNKTYLGRPWKQYSRTVYMQTFIDGF+NP GWDPWSGEYLSTLYY 
Sbjct: 430 TIRATSDLAASKSYMNKTYLGRPWKQYSRTVYMQTFIDGFINPAGWDPWSGEYLSTLYYA 489

Query: 490 EYNNTGGGSDTKNRVIWPGYHVINNATDAANFTISNFLVGDVWLPPTGVPYTGGFV 545
           EYNNTG GS+TKNRV WPGYHVI+NATDA+NFT+SNFLVGD WLPPTGVPYTGGF+
Sbjct: 490 EYNNTGAGSNTKNRVTWPGYHVISNATDASNFTVSNFLVGDAWLPPTGVPYTGGFI 543

BLAST of IVF0022449 vs. TAIR 10
Match: AT2G47550.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 626.3 bits (1614), Expect = 2.3e-179
Identity = 318/548 (58.03%), Postives = 403/548 (73.54%), Query Frame = 0

Query: 3   YLDSIYDSLTNIFPTKAICKLTSNPSYCITVLKQSRDGNIYDSGRFSIRRSLSKASRFLD 62
           ++ +   ++ N      IC+   +PSYC +VL  ++ G+IY  GR S+RRSLS+A RF+ 
Sbjct: 21  FISAQIPAIGNATSPSNICRFAPDPSYCRSVL-PNQPGDIYSYGRLSLRRSLSRARRFIS 80

Query: 63  LIEKHL-QNSSTLPKSIIGALKDCQYLAQLNMNFLSNSFRAVNGTDRKLTYSKADYIQSL 122
           +I+  L +      KS +GAL+DC++LA L M++L +S +  + T + L+ S+A+ + + 
Sbjct: 81  MIDAELDRKGKVAAKSTVGALEDCKFLASLTMDYLLSSSQTADST-KTLSLSRAEDVHTF 140

Query: 123 LSAILTNIDTCLDGLNTVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPRWNRNRTL 182
           LSA +TN  TCL+GL + AS + L  D    L + TK Y +SL LF+KGWVPR  R+R +
Sbjct: 141 LSAAITNEQTCLEGLKSTASENGLSGD----LFNDTKLYGVSLALFSKGWVPRRQRSRPI 200

Query: 183 EHPG---KKHLQFRKGPLPLRMSRHDRAVYNSVANRRKLSSSSDDGVLVNGIAVVSQDGE 242
             P    KK   FR G LPL+M+   RAVYN+V  RRKL  S  D V V+ I  V Q+G 
Sbjct: 201 WQPQARFKKFFGFRNGKLPLKMTERARAVYNTV-TRRKLLQSDADAVQVSDIVTVIQNGT 260

Query: 243 GDFLNITDAINAAPNNSLAANGYFLIYITAGVYQEYVSVPSKKKYLLMIGDGINQTIITG 302
           G+F  I  AI AAPN +  +NGYFLIY+TAG+Y+EYV VP  K+Y++MIGDGINQT+ITG
Sbjct: 261 GNFTTINAAIAAAPNKTDGSNGYFLIYVTAGLYEEYVEVPKNKRYVMMIGDGINQTVITG 320

Query: 303 NRSVADGWTTFNSATFAVAAEGFMAVNITFQNTAGAIKGQAVALRSGADMCVFYSCSFEG 362
           NRSV DGWTTFNSATF ++   F+ VNIT +NTAG  KGQAVALRSG D+ VFYSCSFE 
Sbjct: 321 NRSVVDGWTTFNSATFILSGPNFIGVNITIRNTAGPTKGQAVALRSGGDLSVFYSCSFEA 380

Query: 363 YQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPGPRQANMITAQGRSDP 422
           YQDTLYTHSLRQF+RECD+YGTVDFIFGNAAVV QNCN+YPR P   Q+N +TAQGR+DP
Sbjct: 381 YQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCNLYPRQPRKGQSNEVTAQGRTDP 440

Query: 423 NQNTGTSIHNCTIQATPDLAASSSHMNKTYLGRPWKQYSRTVYMQTFIDGFVNPKGWDPW 482
           NQNTGT+IH CTI+   DL A+S++  KTYLGRPWK+YSRTV MQT+IDGF+ P GW+ W
Sbjct: 441 NQNTGTAIHGCTIRPADDL-ATSNYTVKTYLGRPWKEYSRTVVMQTYIDGFLEPSGWNAW 500

Query: 483 SGEY-LSTLYYGEYNNTGGGSDTKNRVIWPGYHVINNATDAANFTISNFLVGDVWLPPTG 542
           SG++ LSTLYY EYNNTG GSDT NRV WPGYHVI NATDA+NFT++NFLVG+ W+  TG
Sbjct: 501 SGDFALSTLYYAEYNNTGPGSDTTNRVTWPGYHVI-NATDASNFTVTNFLVGEGWIGQTG 559

Query: 543 VPYTGGFV 546
           VP+ GG +
Sbjct: 561 VPFVGGLI 559

BLAST of IVF0022449 vs. TAIR 10
Match: AT1G02810.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 620.2 bits (1598), Expect = 1.6e-177
Identity = 322/568 (56.69%), Postives = 410/568 (72.18%), Query Frame = 0

Query: 3   YLDSIYDSLTNIFPTKAICKLTSNPSYCITVLKQSRDGNIYDSGRFSIRRSLSKASRFLD 62
           +L S+  S   +  +  ICK T +P YC +V   S+ GN+   G FSIR+SLS++ +F+ 
Sbjct: 14  FLQSVLASSQTLSNSSTICKTTPDPKYCKSVFPHSQ-GNVQQYGCFSIRKSLSQSRKFIR 73

Query: 63  LIEKHLQNSSTLPK-SIIGALKDCQYLAQLNMNFLSNSFRAVNGTDRK-----LTYSKAD 122
            ++++++ ++ L + ++I AL+DC++LA L M++L  SF  VN T  K     L++ KAD
Sbjct: 74  TVDRYIKRNAHLSQPAVIRALQDCRFLAGLTMDYLLTSFETVNDTSAKTSFKPLSFPKAD 133

Query: 123 YIQSLLSAILTNIDTCLDGLNTVASGS---SLEKDLLAPLIDCTKSYSLSLDLFTKGWVP 182
            IQ+LLSA LTN  TCL+GL T AS S   ++   +  PL++ TK   +SL LFTKGWVP
Sbjct: 134 DIQTLLSAALTNEQTCLEGLTTAASYSATWTVRTGVALPLVNDTKLLGVSLALFTKGWVP 193

Query: 183 R--------WNRNRTLEHPGKKHLQ-FRKGPLPLRMSRHDRAVYNSVANRRKLSSSS--- 242
           +        W + R+      K  + FR G LPL+M+   +AVY S++ R+     S   
Sbjct: 194 KKKKRAGFAWAQPRSGSSTHTKPFRLFRNGALPLKMTEKTKAVYESLSRRKLADGDSNGD 253

Query: 243 -DDG--VLVNGIAVVSQDGEGDFLNITDAINAAPNNSLAANGYFLIYITAGVYQEYVSVP 302
            DDG  VL++ I  VSQDG G+F NIT A+ AAPNN+  + G+FLIY+TAG+Y+EY+S+ 
Sbjct: 254 GDDGSMVLISDIVTVSQDGTGNFTNITAAVAAAPNNTDGSAGFFLIYVTAGIYEEYISIA 313

Query: 303 SKKKYLLMIGDGINQTIITGNRSVADGWTTFNSATFAVAAEGFMAVNITFQNTAGAIKGQ 362
             K+Y++MIGDGINQT++TGNRSV DGWTTFNSATFAV A  F+AVNITF+NTAG  K Q
Sbjct: 314 KNKRYMMMIGDGINQTVVTGNRSVVDGWTTFNSATFAVTAPNFVAVNITFRNTAGPEKHQ 373

Query: 363 AVALRSGADMCVFYSCSFEGYQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIY 422
           AVALRSGAD  +FYSCSFE YQDTLYTHSLRQF+RECD+YGTVDFIFGNAAVVFQNCN+Y
Sbjct: 374 AVALRSGADFSIFYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVFQNCNLY 433

Query: 423 PRLPGPRQANMITAQGRSDPNQNTGTSIHNCTIQATPDLAASSSHMNKTYLGRPWKQYSR 482
           PR P P Q N ITAQGRSDPNQNTGTSI NCTI+   DL  SS++  KTYLGRPWK+YSR
Sbjct: 434 PRKPMPNQFNAITAQGRSDPNQNTGTSIQNCTIKPADDL-VSSNYTVKTYLGRPWKEYSR 493

Query: 483 TVYMQTFIDGFVNPKGWDPWSGEY-LSTLYYGEYNNTGGGSDTKNRVIWPGYHVINNATD 542
           TVYMQ++IDGFV P GW  W+G++ LSTLYY EYNNTG GS+T NRV WPGYHVI N+TD
Sbjct: 494 TVYMQSYIDGFVEPVGWREWNGDFALSTLYYAEYNNTGPGSNTTNRVTWPGYHVI-NSTD 553

Query: 543 AANFTISNFLVGDVWLPPTGVPYTGGFV 546
           AANFT++   +   W+  TGVPYT G +
Sbjct: 554 AANFTVTGLFIEADWIWKTGVPYTSGLI 578

BLAST of IVF0022449 vs. TAIR 10
Match: AT4G02330.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 618.6 bits (1594), Expect = 4.7e-177
Identity = 313/561 (55.79%), Postives = 412/561 (73.44%), Query Frame = 0

Query: 4   LDSIYDSLTNIFPTKA---ICKLTSNPSYCITVLKQSRDGNIYDSGRFSIRRSLSKASRF 63
           L +++ +   + P+ +   ICK T +P +C +V  Q+  G++ + GRFS+R+SL+++ +F
Sbjct: 15  LQTLFIASQTLLPSNSSSTICKTTPDPKFCKSVFPQTSQGDVREYGRFSLRKSLTQSRKF 74

Query: 64  LDLIEKHL-QNSSTLPKSIIGALKDCQYLAQLNMNFLSNSFRAVNGT--DRKLTYSKADY 123
              I+++L +N++ L +S +GAL+DC+YLA L  ++L  SF  VN T   + L++SKAD 
Sbjct: 75  TRTIDRYLKRNNALLSQSAVGALQDCRYLASLTTDYLITSFETVNITTSSKTLSFSKADE 134

Query: 124 IQSLLSAILTNIDTCLDGLNTVASGS-SLEKDLLAPLIDCTKSYSLSLDLFTKGWVPR-- 183
           IQ+LLSA LTN  TCLDG+NT AS S ++   +  PLI+ TK +S+SL LFTKGWVP+  
Sbjct: 135 IQTLLSAALTNEQTCLDGINTAASSSWTIRNGVALPLINDTKLFSVSLALFTKGWVPKKK 194

Query: 184 -------WNRNRTLEHPGKKHLQFRKGPLPLRMSRHDRAVYNSVANRRKLSSSSDD--GV 243
                  W   +      K    FR G LPL+M+ H RAVY S+ +RRKL+   +D   V
Sbjct: 195 KQVASYSWAHPKNTHSHTKPFRHFRNGALPLKMTEHTRAVYESL-SRRKLADDDNDVNTV 254

Query: 244 LVNGIAVVSQDGEGDFLNITDAINAAPNNSLAANGYFLIYITAGVYQEYVSVPSKKKYLL 303
           LV+ I  V+Q+G G+F  IT+A+N+APN +    GYF+IY+T+GVY+E V +   K+YL+
Sbjct: 255 LVSDIVTVNQNGTGNFTTITEAVNSAPNKTDGTAGYFVIYVTSGVYEENVVIAKNKRYLM 314

Query: 304 MIGDGINQTIITGNRSVADGWTTFNSATFAVAAEGFMAVNITFQNTAGAIKGQAVALRSG 363
           MIGDGIN+T++TGNR+V DGWTTFNSATFAV +  F+AVN+TF+NTAG  K QAVA+RS 
Sbjct: 315 MIGDGINRTVVTGNRNVVDGWTTFNSATFAVTSPNFVAVNMTFRNTAGPEKHQAVAMRSS 374

Query: 364 ADMCVFYSCSFEGYQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPGPR 423
           AD+ +FYSCSFE YQDTLYTHSLRQF+RECDIYGTVDFIFGNAAVVFQ+CN+YPR P   
Sbjct: 375 ADLSIFYSCSFEAYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVFQDCNLYPRQPMQN 434

Query: 424 QANMITAQGRSDPNQNTGTSIHNCTIQATPDLAASSSHMNKTYLGRPWKQYSRTVYMQTF 483
           Q N ITAQGR+DPNQNTG SIHNCTI+   DL  SS++  KTYLGRPWK+YSRTV+MQ++
Sbjct: 435 QFNAITAQGRTDPNQNTGISIHNCTIKPADDL-VSSNYTVKTYLGRPWKEYSRTVFMQSY 494

Query: 484 IDGFVNPKGWDPWSGEY-LSTLYYGEYNNTGGGSDTKNRVIWPGYHVINNATDAANFTIS 543
           ID  V P GW  W+G++ LSTLYY EYNNTG GS T +RV+WPGYHVI N+TDA NFT+ 
Sbjct: 495 IDEVVEPVGWREWNGDFALSTLYYAEYNNTGSGSSTTDRVVWPGYHVI-NSTDANNFTVE 554

Query: 544 NFLVGDVWLPPTGVPYTGGFV 546
           NFL+GD W+  +GVPY  G +
Sbjct: 555 NFLLGDGWMVQSGVPYISGLL 572

BLAST of IVF0022449 vs. TAIR 10
Match: AT3G10720.2 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 460.3 bits (1183), Expect = 2.2e-129
Identity = 254/540 (47.04%), Postives = 344/540 (63.70%), Query Frame = 0

Query: 21  CKLTSNPSYCITVLK--QSRDGNIYDSGRFSIRRSLSKASRFLDLIEKHLQNSSTLPKSI 80
           CK T  P  C T+L   +S   + Y  G+F+I++ L +ASR   +I  + +   + P S 
Sbjct: 83  CKSTPYPKLCRTILNAVKSSPSDPYRYGKFTIKQCLKQASRLSKVITSYARRVESKPGSA 142

Query: 81  ----IGALKDCQYLAQLNMNFLSNSFRAVNGTDRKLTYSKADYIQSLLSAILTNIDTCLD 140
               IGA+ DC  L++L++N+L      +  T + +T +  +++ SLLS ++TN  TCLD
Sbjct: 143 TAEEIGAVADCGELSELSVNYLETVTTELK-TAQVMTAALVEHVNSLLSGVVTNQQTCLD 202

Query: 141 GLNTVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPRWNRNRTLEHP--GKKHLQFR 200
           GL  V + S     + +P+ + T+ YS+SL L +        R +  +    G  +  +R
Sbjct: 203 GL--VEAKSGFAAAIGSPMGNLTRLYSISLGLVSHALNRNLKRFKASKGKILGGGNSTYR 262

Query: 201 KGPLPL------RMSRHDRAVYNSVANRRKLSSSSDDGVLVNGIAVVSQDGEGDFLNITD 260
           + PL        +   +D+    +  N  +L  +S   +LV+   +V      +F  ITD
Sbjct: 263 E-PLETLIKGLRKTCDNDKDCRKTSRNLGELGETSGGSILVSKAVIVGPFKSDNFTTITD 322

Query: 261 AINAAPNNSLAANGYFLIYITAGVYQEYVSVPSKKKYLLMIGDGINQTIITGNRSVADGW 320
           AI AAPNN+   +GYF+IY   GVY+EY+ VP  KK L+++GDGIN+TIITGN +V DGW
Sbjct: 323 AIAAAPNNTRPEDGYFVIYAREGVYEEYIVVPINKKNLMLMGDGINKTIITGNHNVVDGW 382

Query: 321 TTFNSATFAVAAEGFMAVNITFQNTAGAIKGQAVALRSGADMCVFYSCSFEGYQDTLYTH 380
           TT+N ++FAV  E FMAV++TF+NTAG  K QAVALR+ A+   FY CSFEGYQDTLY H
Sbjct: 383 TTYNCSSFAVVGERFMAVDVTFRNTAGPEKHQAVALRNNAEGSSFYRCSFEGYQDTLYVH 442

Query: 381 SLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPGPRQANMITAQGRSDPNQNTGTSI 440
           SLRQF+RECDIYGTVDFIFGNAA +FQNCNIY R P  +Q N ITA GR DPNQNTG SI
Sbjct: 443 SLRQFYRECDIYGTVDFIFGNAAAIFQNCNIYARKPMAKQKNAITAHGRLDPNQNTGISI 502

Query: 441 HNCTIQATPDLAASSSHMNKTYLGRPWKQYSRTVYMQTFIDGFVNPKGWDPWSGEY-LST 500
            NCTI+A PDLAA       T+LGRPWK YSRTV+MQ++I   V P GW  W+G   L T
Sbjct: 503 INCTIKAAPDLAAEPKSA-MTFLGRPWKPYSRTVFMQSYISDIVQPVGWLEWNGTIGLDT 562

Query: 501 LYYGEYNNTGGGSDTKNRVIWPGYHVINNATDAANFTISNFLVGDVWLPPTGVPYTGGFV 546
           +YYGEY+N G G++T  RV W GY+++N A +A NFT+ NF +GD WLP T +P+ GG +
Sbjct: 563 IYYGEYSNFGPGANTNQRVQWLGYNLLNLA-EAMNFTVYNFTMGDTWLPQTDIPFYGGLL 616

BLAST of IVF0022449 vs. TAIR 10
Match: AT5G04970.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 442.2 bits (1136), Expect = 6.1e-124
Identity = 248/549 (45.17%), Postives = 330/549 (60.11%), Query Frame = 0

Query: 16  PTKAICKLTSNPSYCITVLK--QSRDGNIYDSGRFSIRRSLSKASRFLDLIEKHLQNSST 75
           P    CK T  P  C T+L   +S   + Y  G+F++++ L +A R   +I +  Q    
Sbjct: 83  PPNIACKSTPYPKLCRTILSAVKSSPSDPYHYGKFTMKQCLKQARRLSKVINRFAQRVEA 142

Query: 76  LPKSI----IGALKDCQYLAQLNMNFLSNSFRAVNGTDRKLTYSKADYIQSLLSAILTNI 135
            P +     + A+ DC  LA+L++ +L      +   +  +T +  D + SLL  ++TN 
Sbjct: 143 DPGTSTVEEVSAVADCGELAELSVEYLETVTEELKAAE-LMTAALVDRVTSLLGGVVTNQ 202

Query: 136 DTCLDGLNTVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPRWNRNRTLEHPGKKHL 195
            TCLDGL    SG +    +  PL + T+ YS+SL L +       NRN    + G K  
Sbjct: 203 QTCLDGLVDAKSGFATA--IGTPLGNLTRLYSVSLGLVSHA----LNRN-LKRYKGSKGK 262

Query: 196 QFRKGPLPLR----------MSRHDRAVYNSVANRR--KLSSSSDDGVLVNGIAVVSQDG 255
            F  G  P+R              D+      ANR   +L  +S   +LV     V    
Sbjct: 263 IFGGGNKPVREPLETLIKVLRKTCDKGKDCRKANRNLGELGETSGGSILVREAVTVGPYE 322

Query: 256 EGDFLNITDAINAAPNNSLAANGYFLIYITAGVYQEYVSVPSKKKYLLMIGDGINQTIIT 315
             +F  IT+A+ AAPN++    GYF+IY  AG+Y+EYV + +KK+ +++IGDGIN+TII+
Sbjct: 323 TDNFPTITEAVAAAPNHTFPEQGYFVIYARAGLYEEYVVISNKKRNIMLIGDGINKTIIS 382

Query: 316 GNRSVADGWTTFNSATFAVAAEGFMAVNITFQNTAGAIKGQAVALRSGADMCVFYSCSFE 375
           GN S  DGWTT+NS+TFAV  + F+AV++TF+NTAG  K QAVA+R+ AD   FY CSFE
Sbjct: 383 GNHSFIDGWTTYNSSTFAVVGDRFVAVDVTFRNTAGPEKHQAVAVRNNADGSTFYRCSFE 442

Query: 376 GYQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPGPRQANMITAQGRSD 435
           GYQDTLY HSLRQF+RECDIYGT+DFIFGNAA +FQNCNIY R P   Q N +TA GR+D
Sbjct: 443 GYQDTLYVHSLRQFYRECDIYGTIDFIFGNAAAIFQNCNIYARKPMANQKNAVTAHGRTD 502

Query: 436 PNQNTGTSIHNCTIQATPDLAASSSHMNKTYLGRPWKQYSRTVYMQTFIDGFVNPKGWDP 495
           PNQ TG SI NCTI A PDLAA       T+LGRPWK YSRTVY+Q++I   V P GW  
Sbjct: 503 PNQKTGISIINCTIGAAPDLAADPK-STMTFLGRPWKPYSRTVYIQSYISDVVQPVGWLE 562

Query: 496 WSGEY-LSTLYYGEYNNTGGGSDTKNRVIWPGYHVINNATDAANFTISNFLVGDVWLPPT 546
           W+G   L T+ YGEY+N G G+DT  RV W GY ++ N   A NFT+ NF +GD WLP T
Sbjct: 563 WNGTTGLDTISYGEYDNFGPGADTSKRVQWSGYSLL-NLVQAMNFTVYNFTLGDTWLPQT 621