IVF0020627 (gene) Melon (IVF77) v1

Overview
NameIVF0020627
Typegene
OrganismCucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
DescriptionRibonuclease H
Locationchr03: 5247510 .. 5247800 (-)
RNA-Seq ExpressionIVF0020627
SyntenyIVF0020627
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCGGTCACTTATAAAGATTGGCATGAAATGCTACCTTTCACATTGCATGGATATAGAATATCAGTTCGCACGTCAACAGGGGCAATCCCTTTTTCACTAGCGTATGGTTTCGAAGCAGTTTTACCTGTTGAAGTTGAGGTGCCATTTCTTAGGGTAATCCAAGAGGTAGAACTAGATGAAGCAAAGTGGACTCAAGTAAGGTACGAGCAATTGAATTTCATAGAAGAAAGGAGACTGACTGCACTATGTAGAGGACAGTTGAACCAAAAGAAAATAGCACGAGCGTAG

mRNA sequence

ATGTCGGTCACTTATAAAGATTGGCATGAAATGCTACCTTTCACATTGCATGGATATAGAATATCAGTTCGCACGTCAACAGGGGCAATCCCTTTTTCACTAGCGTATGGTTTCGAAGCAGTTTTACCTGTTGAAGTTGAGGTGCCATTTCTTAGGGTAATCCAAGAGGTAGAACTAGATGAAGCAAAGTGGACTCAAGTAAGGTACGAGCAATTGAATTTCATAGAAGAAAGGAGACTGACTGCACTATGTAGAGGACAGTTGAACCAAAAGAAAATAGCACGAGCGTAG

Coding sequence (CDS)

ATGTCGGTCACTTATAAAGATTGGCATGAAATGCTACCTTTCACATTGCATGGATATAGAATATCAGTTCGCACGTCAACAGGGGCAATCCCTTTTTCACTAGCGTATGGTTTCGAAGCAGTTTTACCTGTTGAAGTTGAGGTGCCATTTCTTAGGGTAATCCAAGAGGTAGAACTAGATGAAGCAAAGTGGACTCAAGTAAGGTACGAGCAATTGAATTTCATAGAAGAAAGGAGACTGACTGCACTATGTAGAGGACAGTTGAACCAAAAGAAAATAGCACGAGCGTAG

Protein sequence

MSVTYKDWHEMLPFTLHGYRISVRTSTGAIPFSLAYGFEAVLPVEVEVPFLRVIQEVELDEAKWTQVRYEQLNFIEERRLTALCRGQLNQKKIARA
Homology
BLAST of IVF0020627 vs. ExPASy TrEMBL
Match: A0A5A7SVL0 (Pol polyprotein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold845G00080 PE=4 SV=1)

HSP 1 Score: 176.8 bits (447), Expect = 4.4e-41
Identity = 87/96 (90.62%), Postives = 90/96 (93.75%), Query Frame = 0

Query: 1   MSVTYKDWHEMLPFTLHGYRISVRTSTGAIPFSLAYGFEAVLPVEVEVPFLRVIQEVELD 60
           MSVTY+DWHEMLPF LHGYRISVRTSTG+ PFSL YGFEAVLPVEVEVP LRVIQE+ELD
Sbjct: 40  MSVTYRDWHEMLPFALHGYRISVRTSTGSTPFSLVYGFEAVLPVEVEVPSLRVIQELELD 99

Query: 61  EAKWTQVRYEQLNFIEERRLTALCRGQLNQKKIARA 97
           EAKW QVRYEQLNFIEERRLTALCRGQL QKKIARA
Sbjct: 100 EAKWAQVRYEQLNFIEERRLTALCRGQLYQKKIARA 135

BLAST of IVF0020627 vs. ExPASy TrEMBL
Match: A0A5A7VAF2 (RNA-directed DNA polymerase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold1023G00050 PE=4 SV=1)

HSP 1 Score: 174.1 bits (440), Expect = 2.8e-40
Identity = 86/96 (89.58%), Postives = 90/96 (93.75%), Query Frame = 0

Query: 1   MSVTYKDWHEMLPFTLHGYRISVRTSTGAIPFSLAYGFEAVLPVEVEVPFLRVIQEVELD 60
           MSVTY+DWHEMLPF L+GYRISVRTSTG+ PFSL YGFEAVLPVEVEVP LRVIQE+ELD
Sbjct: 234 MSVTYRDWHEMLPFALNGYRISVRTSTGSTPFSLVYGFEAVLPVEVEVPSLRVIQELELD 293

Query: 61  EAKWTQVRYEQLNFIEERRLTALCRGQLNQKKIARA 97
           EAKW QVRYEQLNFIEERRLTALCRGQL QKKIARA
Sbjct: 294 EAKWAQVRYEQLNFIEERRLTALCRGQLYQKKIARA 329

BLAST of IVF0020627 vs. ExPASy TrEMBL
Match: A0A5A7TU03 (Pol polyprotein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold114G001560 PE=4 SV=1)

HSP 1 Score: 164.5 bits (415), Expect = 2.2e-37
Identity = 79/88 (89.77%), Postives = 83/88 (94.32%), Query Frame = 0

Query: 1  MSVTYKDWHEMLPFTLHGYRISVRTSTGAIPFSLAYGFEAVLPVEVEVPFLRVIQEVELD 60
          MSVTY+DWHEMLPF LHGYRISVRTSTG+ PFSL YGFEAVLPVEVEVP LRVIQE++LD
Sbjct: 1  MSVTYRDWHEMLPFALHGYRISVRTSTGSTPFSLVYGFEAVLPVEVEVPSLRVIQELKLD 60

Query: 61 EAKWTQVRYEQLNFIEERRLTALCRGQL 89
          EAKW QVRYEQLNFIEERRLTALCRGQL
Sbjct: 61 EAKWAQVRYEQLNFIEERRLTALCRGQL 88

BLAST of IVF0020627 vs. ExPASy TrEMBL
Match: A0A5A7UZT9 (Pol polyprotein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold35127G00010 PE=4 SV=1)

HSP 1 Score: 163.7 bits (413), Expect = 3.8e-37
Identity = 81/94 (86.17%), Postives = 85/94 (90.43%), Query Frame = 0

Query: 1  MSVTYKDWHEMLPFTLHGYRISVRTSTGAIPFSLAYGFEAVLPVEVEVPFLRVIQEVELD 60
          MSVTY+DWHEMLPF LHGYRISVRTSTG+ P SL YGFEAVLPVEVEV  LRVIQEVELD
Sbjct: 1  MSVTYRDWHEMLPFALHGYRISVRTSTGSTPLSLVYGFEAVLPVEVEVSSLRVIQEVELD 60

Query: 61 EAKWTQVRYEQLNFIEERRLTALCRGQLNQKKIA 95
          EAKW QVRY+QLNFIEER LTALC+GQL QKKIA
Sbjct: 61 EAKWAQVRYKQLNFIEERILTALCKGQLYQKKIA 94

BLAST of IVF0020627 vs. ExPASy TrEMBL
Match: A0A5D3CZI6 (Pol polyprotein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold637G00430 PE=4 SV=1)

HSP 1 Score: 156.0 bits (393), Expect = 8.0e-35
Identity = 78/96 (81.25%), Postives = 82/96 (85.42%), Query Frame = 0

Query: 1   MSVTYKDWHEMLPFTLHGYRISVRTSTGAIPFSLAYGFEAVLPVEVEVPFLRVIQEVELD 60
           M V Y+DWHEMLPF L+GYRISVRTSTGA  FSL YGFE +LP+EVEV  L VIQEVELD
Sbjct: 51  MFVAYRDWHEMLPFALYGYRISVRTSTGATAFSLVYGFETILPIEVEVLSLGVIQEVELD 110

Query: 61  EAKWTQVRYEQLNFIEERRLTALCRGQLNQKKIARA 97
           E KW QVRYEQLNFIEERRLTALCRGQL QKKIA A
Sbjct: 111 EEKWAQVRYEQLNFIEERRLTALCRGQLYQKKIAGA 146

BLAST of IVF0020627 vs. NCBI nr
Match: KAA0033219.1 (Pol polyprotein [Cucumis melo var. makuwa])

HSP 1 Score: 176 bits (447), Expect = 7.65e-54
Identity = 87/96 (90.62%), Postives = 90/96 (93.75%), Query Frame = 0

Query: 1   MSVTYKDWHEMLPFTLHGYRISVRTSTGAIPFSLAYGFEAVLPVEVEVPFLRVIQEVELD 60
           MSVTY+DWHEMLPF LHGYRISVRTSTG+ PFSL YGFEAVLPVEVEVP LRVIQE+ELD
Sbjct: 40  MSVTYRDWHEMLPFALHGYRISVRTSTGSTPFSLVYGFEAVLPVEVEVPSLRVIQELELD 99

Query: 61  EAKWTQVRYEQLNFIEERRLTALCRGQLNQKKIARA 96
           EAKW QVRYEQLNFIEERRLTALCRGQL QKKIARA
Sbjct: 100 EAKWAQVRYEQLNFIEERRLTALCRGQLYQKKIARA 135

BLAST of IVF0020627 vs. NCBI nr
Match: KAA0065292.1 (RNA-directed DNA polymerase [Cucumis melo var. makuwa])

HSP 1 Score: 174 bits (440), Expect = 1.57e-50
Identity = 86/96 (89.58%), Postives = 90/96 (93.75%), Query Frame = 0

Query: 1   MSVTYKDWHEMLPFTLHGYRISVRTSTGAIPFSLAYGFEAVLPVEVEVPFLRVIQEVELD 60
           MSVTY+DWHEMLPF L+GYRISVRTSTG+ PFSL YGFEAVLPVEVEVP LRVIQE+ELD
Sbjct: 234 MSVTYRDWHEMLPFALNGYRISVRTSTGSTPFSLVYGFEAVLPVEVEVPSLRVIQELELD 293

Query: 61  EAKWTQVRYEQLNFIEERRLTALCRGQLNQKKIARA 96
           EAKW QVRYEQLNFIEERRLTALCRGQL QKKIARA
Sbjct: 294 EAKWAQVRYEQLNFIEERRLTALCRGQLYQKKIARA 329

BLAST of IVF0020627 vs. NCBI nr
Match: KAA0046610.1 (Pol polyprotein [Cucumis melo var. makuwa])

HSP 1 Score: 164 bits (414), Expect = 1.90e-50
Identity = 79/88 (89.77%), Postives = 83/88 (94.32%), Query Frame = 0

Query: 1  MSVTYKDWHEMLPFTLHGYRISVRTSTGAIPFSLAYGFEAVLPVEVEVPFLRVIQEVELD 60
          MSVTY+DWHEMLPF LHGYRISVRTSTG+ PFSL YGFEAVLPVEVEVP LRVIQE++LD
Sbjct: 1  MSVTYRDWHEMLPFALHGYRISVRTSTGSTPFSLVYGFEAVLPVEVEVPSLRVIQELKLD 60

Query: 61 EAKWTQVRYEQLNFIEERRLTALCRGQL 88
          EAKW QVRYEQLNFIEERRLTALCRGQL
Sbjct: 61 EAKWAQVRYEQLNFIEERRLTALCRGQL 88

BLAST of IVF0020627 vs. NCBI nr
Match: KAA0060778.1 (Pol polyprotein [Cucumis melo var. makuwa])

HSP 1 Score: 163 bits (413), Expect = 3.15e-50
Identity = 81/94 (86.17%), Postives = 85/94 (90.43%), Query Frame = 0

Query: 1  MSVTYKDWHEMLPFTLHGYRISVRTSTGAIPFSLAYGFEAVLPVEVEVPFLRVIQEVELD 60
          MSVTY+DWHEMLPF LHGYRISVRTSTG+ P SL YGFEAVLPVEVEV  LRVIQEVELD
Sbjct: 1  MSVTYRDWHEMLPFALHGYRISVRTSTGSTPLSLVYGFEAVLPVEVEVSSLRVIQEVELD 60

Query: 61 EAKWTQVRYEQLNFIEERRLTALCRGQLNQKKIA 94
          EAKW QVRY+QLNFIEER LTALC+GQL QKKIA
Sbjct: 61 EAKWAQVRYKQLNFIEERILTALCKGQLYQKKIA 94

BLAST of IVF0020627 vs. NCBI nr
Match: TYK15866.1 (Pol polyprotein [Cucumis melo var. makuwa])

HSP 1 Score: 155 bits (393), Expect = 4.89e-46
Identity = 78/96 (81.25%), Postives = 82/96 (85.42%), Query Frame = 0

Query: 1   MSVTYKDWHEMLPFTLHGYRISVRTSTGAIPFSLAYGFEAVLPVEVEVPFLRVIQEVELD 60
           M V Y+DWHEMLPF L+GYRISVRTSTGA  FSL YGFE +LP+EVEV  L VIQEVELD
Sbjct: 51  MFVAYRDWHEMLPFALYGYRISVRTSTGATAFSLVYGFETILPIEVEVLSLGVIQEVELD 110

Query: 61  EAKWTQVRYEQLNFIEERRLTALCRGQLNQKKIARA 96
           E KW QVRYEQLNFIEERRLTALCRGQL QKKIA A
Sbjct: 111 EEKWAQVRYEQLNFIEERRLTALCRGQLYQKKIAGA 146

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A5A7SVL04.4e-4190.63Pol polyprotein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold845G0008... [more]
A0A5A7VAF22.8e-4089.58RNA-directed DNA polymerase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaf... [more]
A0A5A7TU032.2e-3789.77Pol polyprotein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold114G0015... [more]
A0A5A7UZT93.8e-3786.17Pol polyprotein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold35127G00... [more]
A0A5D3CZI68.0e-3581.25Pol polyprotein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold637G0043... [more]
Match NameE-valueIdentityDescription
KAA0033219.17.65e-5490.63Pol polyprotein [Cucumis melo var. makuwa][more]
KAA0065292.11.57e-5089.58RNA-directed DNA polymerase [Cucumis melo var. makuwa][more]
KAA0046610.11.90e-5089.77Pol polyprotein [Cucumis melo var. makuwa][more]
KAA0060778.13.15e-5086.17Pol polyprotein [Cucumis melo var. makuwa][more]
TYK15866.14.89e-4681.25Pol polyprotein [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 1..83
e-value: 8.5E-7
score: 30.4

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
IVF0020627.1IVF0020627.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015074 DNA integration
biological_process GO:0006310 DNA recombination
biological_process GO:0006278 RNA-dependent DNA biosynthetic process
biological_process GO:0090502 RNA phosphodiester bond hydrolysis, endonucleolytic
cellular_component GO:0030430 host cell cytoplasm
cellular_component GO:0005634 nucleus
molecular_function GO:0003887 DNA-directed DNA polymerase activity
molecular_function GO:0003723 RNA binding
molecular_function GO:0003964 RNA-directed DNA polymerase activity
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity
molecular_function GO:0003676 nucleic acid binding