IVF0020566 (gene) Melon (IVF77) v1

Overview


Name	IVF0020566
Type	gene
Organism	Cucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
Description	Photosystem I reaction centre subunit N
Location	chr01: 914463 .. 915922 (-)
RNA-Seq Expression	IVF0020566
Synteny	IVF0020566

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAATTTTGTTATATGTGACCAAGAAAGCCTCATCTCTTGTTGAACCTCATCCTCAAACTCAAAGAAAAAAAACTACCACTCTCCTCCCCATGAGTTCCATAGGCCAAAGCATTCTGATGGCCCTCGCCGTCACTCTCAACAAATTTGCTTCTTCCAACGTTCAATCCGTTCAAAGAAACAAAGCCACCGCCACCGTTTCTTCACCAATTGGAAGAAGAGACCTCCTCTTGTCCACTGTCGCCCCCGCCTCCACCGCCGCCGCCGCCGCCGCGGTCGACTCCAGAACTGAGCTGCTAAAAAGTGGTGCATTCTTCTCTAGCTAGCTTCGTTCTTCCTTTTGGGAGTTTTAATTTCTCTTCTTTCATCAATTTTGCGTTTTACTTTTGAAAATTGTTATTTGTTTCTTTGTTATCTTCCCCAAATATCCATTTTCTTCTTCTTCTTCTTCTCCTGAAGTCTAATTTTGGGCGAAGCATCCAACTGAGTTCATCTGTTTTCGTTATCAATTTACAAATTTTTTGCTCTTTTCTAGAATATCGCAATTTTATTTATTTATTTATTTTGAAAAATAATTTCAAATTTATTAAAATTTTGTTTTTATTTCTTTCCATCCAAAAAAAAAAAATCTTTTTTTAACAATTTTATGTTCTAAAATGGTTTTAGTGAATTATTCAACACTTAGCAAATGAATTTTTTTTTTTAATTACAAAAGTTGGGTTTTAAATATGTTATGTATTAAAAAGAAAATTCATATTGTAGAGCATTAAAAACAATTATAATGTACTTGTAATTAAAAAAAAAAAGATTTCTATTTTTAAAATAAATAATTATATTGAATAGTGTTATATAAAATATTTGTAAGTATTGTTTATATGTTAAATAATTGATTGATTTTTTATGTGTGTGTGTGTATATATATATATATAGATTTTTAGAGTTTTGGAAATTGGAAATTTAGTTCCTAATAATATGTTTAGTGTTTGACAATTAGTATCTAATAATTATTACAATTTCCTCTAATTATTGACTTTTGTATTTAGGGTACCTCAAGAAGTCTGAAGAAAACAAAGAAAAGAATGACAAGGAGGTAATTTTTAATTTAATAATTTAACCTATTTTTCTAATTGATTAAAAATGTTAAAATAATCCTTATTTTCTTTTTTGAAAAACGATTCAAATGTGATCGTTTGCGGTTTTAGTTACATCGTAAGATATACGATTAAAGATAACATGATTAATTCTTTTGATACAAAATTAACTCTATAATTTTAGCAGAGATTGGAAAGTTACTACAAGCGAAACTACAAAGATTATTTTGAGTTTGTTGAAGGATCAGTGAAGAATAAGAACGAACTTTCGGAAGCTGAAAAAGGTATTGTCGAGTGGCTTAAACGAAATAAATAAAAGAGTATACTTTTTCATTTTTAGTTACCATAAATATAACAATAGTCTTAGTTCAT

mRNA sequence

AAATTTTGTTATATGTGACCAAGAAAGCCTCATCTCTTGTTGAACCTCATCCTCAAACTCAAAGAAAAAAAACTACCACTCTCCTCCCCATGAGTTCCATAGGCCAAAGCATTCTGATGGCCCTCGCCGTCACTCTCAACAAATTTGCTTCTTCCAACGTTCAATCCGTTCAAAGAAACAAAGCCACCGCCACCGTTTCTTCACCAATTGGAAGAAGAGACCTCCTCTTGTCCACTGTCGCCCCCGCCTCCACCGCCGCCGCCGCCGCCGCGGTCGACTCCAGAACTGAGCTGCTAAAAAGGTACCTCAAGAAGTCTGAAGAAAACAAAGAAAAGAATGACAAGGAGAGATTGGAAAGTTACTACAAGCGAAACTACAAAGATTATTTTGAGTTTGTTGAAGGATCAGTGAAGAATAAGAACGAACTTTCGGAAGCTGAAAAAGGTATTGTCGAGTGGCTTAAACGAAATAAATAAAAGAGTATACTTTTTCATTTTTAGTTACCATAAATATAACAATAGTCTTAGTTCAT

Coding sequence (CDS)

ATGAGTTCCATAGGCCAAAGCATTCTGATGGCCCTCGCCGTCACTCTCAACAAATTTGCTTCTTCCAACGTTCAATCCGTTCAAAGAAACAAAGCCACCGCCACCGTTTCTTCACCAATTGGAAGAAGAGACCTCCTCTTGTCCACTGTCGCCCCCGCCTCCACCGCCGCCGCCGCCGCCGCGGTCGACTCCAGAACTGAGCTGCTAAAAAGGTACCTCAAGAAGTCTGAAGAAAACAAAGAAAAGAATGACAAGGAGAGATTGGAAAGTTACTACAAGCGAAACTACAAAGATTATTTTGAGTTTGTTGAAGGATCAGTGAAGAATAAGAACGAACTTTCGGAAGCTGAAAAAGGTATTGTCGAGTGGCTTAAACGAAATAAATAA

Protein sequence

MSSIGQSILMALAVTLNKFASSNVQSVQRNKATATVSSPIGRRDLLLSTVAPASTAAAAAAVDSRTELLKRYLKKSEENKEKNDKERLESYYKRNYKDYFEFVEGSVKNKNELSEAEKGIVEWLKRNK

Homology

[Close]

BLAST of IVF0020566 vs. ExPASy TrEMBL
Match: A0A1S3C176 (uncharacterized protein LOC103495319 OS=Cucumis melo OX=3656 GN=LOC103495319 PE=3 SV=1)

HSP 1 Score: 229.2 bits (583), Expect = 9.9e-57
Identity = 128/128 (100.00%), Postives = 128/128 (100.00%), Query Frame = 0


Query: 1   MSSIGQSILMALAVTLNKFASSNVQSVQRNKATATVSSPIGRRDLLLSTVAPASTAAAAA 60
           MSSIGQSILMALAVTLNKFASSNVQSVQRNKATATVSSPIGRRDLLLSTVAPASTAAAAA
Sbjct: 1   MSSIGQSILMALAVTLNKFASSNVQSVQRNKATATVSSPIGRRDLLLSTVAPASTAAAAA 60

Query: 61  AVDSRTELLKRYLKKSEENKEKNDKERLESYYKRNYKDYFEFVEGSVKNKNELSEAEKGI 120
           AVDSRTELLKRYLKKSEENKEKNDKERLESYYKRNYKDYFEFVEGSVKNKNELSEAEKGI
Sbjct: 61  AVDSRTELLKRYLKKSEENKEKNDKERLESYYKRNYKDYFEFVEGSVKNKNELSEAEKGI 120

Query: 121 VEWLKRNK 129
           VEWLKRNK
Sbjct: 121 VEWLKRNK 128

[Close]

BLAST of IVF0020566 vs. ExPASy TrEMBL
Match: A0A6J1JNZ7 (uncharacterized protein LOC111486201 OS=Cucurbita maxima OX=3661 GN=LOC111486201 PE=3 SV=1)

HSP 1 Score: 179.9 bits (455), Expect = 6.9e-42
Identity = 107/135 (79.26%), Postives = 115/135 (85.19%), Query Frame = 0


Query: 1   MSSIGQSILMALAVTLNKFASSNVQSVQRNK-------ATATVSSPIGRRDLLLSTVAPA 60
           MSSIGQ+ILMALA+TLN+FASSNVQSVQRNK       AT + SS I RR LLLS     
Sbjct: 1   MSSIGQNILMALALTLNQFASSNVQSVQRNKPKTPPTTATTSASSDIQRRGLLLS----- 60

Query: 61  STAAAAAAVDSRTELLKRYLKKSEENKEKNDKERLESYYKRNYKDYFEFVEGSVKNKNEL 120
             AA AAAVDSRTELLKRYLKKSEENKEKNDKERLES+YKRNYKDYFEFVEGS+KNK+EL
Sbjct: 61  --AAVAAAVDSRTELLKRYLKKSEENKEKNDKERLESFYKRNYKDYFEFVEGSLKNKDEL 120

Query: 121 SEAEKGIVEWLKRNK 129
           SEAEKGI+EWLKRNK
Sbjct: 121 SEAEKGIIEWLKRNK 128

[Close]

BLAST of IVF0020566 vs. ExPASy TrEMBL
Match: A0A6J1D574 (uncharacterized protein LOC111017388 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111017388 PE=3 SV=1)

HSP 1 Score: 178.3 bits (451), Expect = 2.0e-41
Identity = 101/128 (78.91%), Postives = 111/128 (86.72%), Query Frame = 0


Query: 1   MSSIGQSILMALAVTLNKFASSNVQSVQRNKATATVSSPIGRRDLLLSTVAPASTAAAAA 60
           MSSIGQSILMALAVT+NKFASSNVQSV RN++ A  +S IGRR LL S V     AAA A
Sbjct: 1   MSSIGQSILMALAVTVNKFASSNVQSVHRNQSAAAAASDIGRRGLLFSAV-----AAAVA 60

Query: 61  AVDSRTELLKRYLKKSEENKEKNDKERLESYYKRNYKDYFEFVEGSVKNKNELSEAEKGI 120
            VDSRTELLKRYLKKSE+NKEKNDKERL+SYYKRNYKDYFEFVEGSV+NK+ELSE EK I
Sbjct: 61  PVDSRTELLKRYLKKSEQNKEKNDKERLDSYYKRNYKDYFEFVEGSVRNKSELSETEKDI 120

Query: 121 VEWLKRNK 129
           +EWL+RNK
Sbjct: 121 IEWLRRNK 123

[Close]

BLAST of IVF0020566 vs. ExPASy TrEMBL
Match: A0A6J1EM63 (uncharacterized protein LOC111434615 OS=Cucurbita moschata OX=3662 GN=LOC111434615 PE=3 SV=1)

HSP 1 Score: 176.4 bits (446), Expect = 7.6e-41
Identity = 106/140 (75.71%), Postives = 115/140 (82.14%), Query Frame = 0


Query: 1   MSSIGQSILMALAVTLNKFASSNVQSVQRNK------------ATATVSSPIGRRDLLLS 60
           MSSIGQ+ILMALA+TLN+FASSNVQSVQRNK            A+ + SS I RR LLLS
Sbjct: 1   MSSIGQNILMALALTLNQFASSNVQSVQRNKPKTPPTTTATTSASTSASSDIQRRGLLLS 60

Query: 61  TVAPASTAAAAAAVDSRTELLKRYLKKSEENKEKNDKERLESYYKRNYKDYFEFVEGSVK 120
                  AA AAAVDSRTELLKRYLKKSEENKEKNDKERLES+YKRNYKDYFEFVEGS+K
Sbjct: 61  -------AAVAAAVDSRTELLKRYLKKSEENKEKNDKERLESFYKRNYKDYFEFVEGSLK 120

Query: 121 NKNELSEAEKGIVEWLKRNK 129
           NK+ELSEAEKGI+EWLKRNK
Sbjct: 121 NKDELSEAEKGIIEWLKRNK 133

[Close]

BLAST of IVF0020566 vs. ExPASy TrEMBL
Match: A0A2I4HLI8 (uncharacterized protein LOC109019213 OS=Juglans regia OX=51240 GN=LOC109019213 PE=3 SV=1)

HSP 1 Score: 153.3 bits (386), Expect = 6.9e-34
Identity = 92/135 (68.15%), Postives = 107/135 (79.26%), Query Frame = 0


Query: 1   MSSIGQSILMALAVTLNKFASSNVQSVQR------NKATATVSSPIGRRDLLLSTVAPAS 60
           MSSIGQSILMAL VT+N+FASSNVQ+V R      +  T T +S IGRR LLLST+    
Sbjct: 1   MSSIGQSILMALTVTVNRFASSNVQAVHRRERKTPSSTTTTTTSDIGRRCLLLSTL---- 60

Query: 61  TAAAAAAVDSRTELLKRYLKKSEENKEKNDKERLESYYKRNYKDYFEFVEGSVK-NKNEL 120
             AA    DSRT+LLK+YLKKSEENK KNDKERL+SYYKRNYKDYFEFVEG+ K N+ +L
Sbjct: 61  -VAAPQVADSRTDLLKQYLKKSEENKSKNDKERLDSYYKRNYKDYFEFVEGASKGNQEQL 120

Query: 121 SEAEKGIVEWLKRNK 129
           SEAEKGI++WL+RNK
Sbjct: 121 SEAEKGIIDWLQRNK 130

[Close]

BLAST of IVF0020566 vs. NCBI nr
Match: XP_008455049.1 (PREDICTED: uncharacterized protein LOC103495319 [Cucumis melo])

HSP 1 Score: 228 bits (580), Expect = 1.17e-74
Identity = 128/128 (100.00%), Postives = 128/128 (100.00%), Query Frame = 0


Query: 1   MSSIGQSILMALAVTLNKFASSNVQSVQRNKATATVSSPIGRRDLLLSTVAPASTAAAAA 60
           MSSIGQSILMALAVTLNKFASSNVQSVQRNKATATVSSPIGRRDLLLSTVAPASTAAAAA
Sbjct: 1   MSSIGQSILMALAVTLNKFASSNVQSVQRNKATATVSSPIGRRDLLLSTVAPASTAAAAA 60

Query: 61  AVDSRTELLKRYLKKSEENKEKNDKERLESYYKRNYKDYFEFVEGSVKNKNELSEAEKGI 120
           AVDSRTELLKRYLKKSEENKEKNDKERLESYYKRNYKDYFEFVEGSVKNKNELSEAEKGI
Sbjct: 61  AVDSRTELLKRYLKKSEENKEKNDKERLESYYKRNYKDYFEFVEGSVKNKNELSEAEKGI 120

Query: 121 VEWLKRNK 128
           VEWLKRNK
Sbjct: 121 VEWLKRNK 128

[Close]

BLAST of IVF0020566 vs. NCBI nr
Match: XP_004136934.1 (uncharacterized protein LOC101214221 [Cucumis sativus] >KAE8645839.1 hypothetical protein Csa_017171 [Cucumis sativus])

HSP 1 Score: 208 bits (529), Expect = 7.23e-67
Identity = 122/130 (93.85%), Postives = 125/130 (96.15%), Query Frame = 0


Query: 1   MSSIGQSILMALAVTLNKFASSNVQSVQRNKATAT--VSSPIGRRDLLLSTVAPASTAAA 60
           MSSIGQSILMALAVTLNKFASSNVQSVQRNKATAT  VSSPIGRR LLLST+APAS AAA
Sbjct: 1   MSSIGQSILMALAVTLNKFASSNVQSVQRNKATATATVSSPIGRRSLLLSTLAPAS-AAA 60

Query: 61  AAAVDSRTELLKRYLKKSEENKEKNDKERLESYYKRNYKDYFEFVEGSVKNKNELSEAEK 120
           A+ VDSRTELLKRYLKKSEENKEKNDKERLESYYKRNYKDYFEFVEGSVKNKNELSEAEK
Sbjct: 61  ASTVDSRTELLKRYLKKSEENKEKNDKERLESYYKRNYKDYFEFVEGSVKNKNELSEAEK 120

Query: 121 GIVEWLKRNK 128
           GIVEWLKR+K
Sbjct: 121 GIVEWLKRSK 129

[Close]

BLAST of IVF0020566 vs. NCBI nr
Match: XP_038887440.1 (uncharacterized protein LOC120077574 [Benincasa hispida])

HSP 1 Score: 191 bits (484), Expect = 5.94e-60
Identity = 114/134 (85.07%), Postives = 117/134 (87.31%), Query Frame = 0


Query: 1   MSSIGQSILMALAVTLNKFASSNVQSVQRNKA------TATVSSPIGRRDLLLSTVAPAS 60
           MSSIGQSILMALAVTLNKFASSNVQSVQRN+A      TAT  S IGRR LLLS VA A+
Sbjct: 1   MSSIGQSILMALAVTLNKFASSNVQSVQRNQANKPRTATATTGSTIGRRGLLLSAVA-AA 60

Query: 61  TAAAAAAVDSRTELLKRYLKKSEENKEKNDKERLESYYKRNYKDYFEFVEGSVKNKNELS 120
            A    AVDSRTELLKRYLKKSEENKEKNDKERLESYYKRNYKDYFEFVEGSVKNKNELS
Sbjct: 61  AATPEEAVDSRTELLKRYLKKSEENKEKNDKERLESYYKRNYKDYFEFVEGSVKNKNELS 120

Query: 121 EAEKGIVEWLKRNK 128
           EAEKGI+EWLKRNK
Sbjct: 121 EAEKGIIEWLKRNK 133

[Close]

BLAST of IVF0020566 vs. NCBI nr
Match: XP_022989008.1 (uncharacterized protein LOC111486201 [Cucurbita maxima])

HSP 1 Score: 178 bits (452), Expect = 3.79e-55
Identity = 107/135 (79.26%), Postives = 115/135 (85.19%), Query Frame = 0


Query: 1   MSSIGQSILMALAVTLNKFASSNVQSVQRNK-------ATATVSSPIGRRDLLLSTVAPA 60
           MSSIGQ+ILMALA+TLN+FASSNVQSVQRNK       AT + SS I RR LLLS     
Sbjct: 1   MSSIGQNILMALALTLNQFASSNVQSVQRNKPKTPPTTATTSASSDIQRRGLLLS----- 60

Query: 61  STAAAAAAVDSRTELLKRYLKKSEENKEKNDKERLESYYKRNYKDYFEFVEGSVKNKNEL 120
             AA AAAVDSRTELLKRYLKKSEENKEKNDKERLES+YKRNYKDYFEFVEGS+KNK+EL
Sbjct: 61  --AAVAAAVDSRTELLKRYLKKSEENKEKNDKERLESFYKRNYKDYFEFVEGSLKNKDEL 120

Query: 121 SEAEKGIVEWLKRNK 128
           SEAEKGI+EWLKRNK
Sbjct: 121 SEAEKGIIEWLKRNK 128

[Close]

BLAST of IVF0020566 vs. NCBI nr
Match: XP_022148828.1 (uncharacterized protein LOC111017388 isoform X1 [Momordica charantia])

HSP 1 Score: 177 bits (448), Expect = 1.31e-54
Identity = 101/128 (78.91%), Postives = 111/128 (86.72%), Query Frame = 0


Query: 1   MSSIGQSILMALAVTLNKFASSNVQSVQRNKATATVSSPIGRRDLLLSTVAPASTAAAAA 60
           MSSIGQSILMALAVT+NKFASSNVQSV RN++ A  +S IGRR LL S VA     AA A
Sbjct: 1   MSSIGQSILMALAVTVNKFASSNVQSVHRNQSAAAAASDIGRRGLLFSAVA-----AAVA 60

Query: 61  AVDSRTELLKRYLKKSEENKEKNDKERLESYYKRNYKDYFEFVEGSVKNKNELSEAEKGI 120
            VDSRTELLKRYLKKSE+NKEKNDKERL+SYYKRNYKDYFEFVEGSV+NK+ELSE EK I
Sbjct: 61  PVDSRTELLKRYLKKSEQNKEKNDKERLDSYYKRNYKDYFEFVEGSVRNKSELSETEKDI 120

Query: 121 VEWLKRNK 128
           +EWL+RNK
Sbjct: 121 IEWLRRNK 123

[Close]

BLAST of IVF0020566 vs. TAIR 10
Match: AT1G49975.1 (INVOLVED IN: photosynthesis; LOCATED IN: photosystem I, chloroplast, thylakoid membrane; EXPRESSED IN: 20 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Photosystem I reaction centre subunit N (InterPro:IPR008796); Has 34 Blast hits to 34 proteins in 14 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 34; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 136.3 bits (342), Expect = 1.7e-32
Identity = 85/134 (63.43%), Postives = 105/134 (78.36%), Query Frame = 0


Query: 1   MSSIGQSILMALAVTLNKFASSNVQSVQRN--KATATVSSP--IGRRDLLLSTVAPASTA 60
           MSSI QSILMAL VT+NK+ASSNVQ+V+RN  K  +  + P  +GRR++L S     ST+
Sbjct: 1   MSSISQSILMALTVTVNKYASSNVQAVRRNDTKRHSLTAPPADLGRRNILFS-----STS 60

Query: 61  AAAAAVDSRTELLKRYLKKSEENKEKNDKERLESYYKRNYKDYFEFVEGSVKNKN--ELS 120
             AAA+ S  +LL++YLKK+EENK KNDKERL+S+YKRNYKDYFEFVEGS+K K   ELS
Sbjct: 61  FIAAALTSSDQLLQKYLKKTEENKAKNDKERLDSFYKRNYKDYFEFVEGSIKGKTEAELS 120

Query: 121 EAEKGIVEWLKRNK 129
           E+EK I+EWLK NK
Sbjct: 121 ESEKRILEWLKANK 129

The following BLAST results are available for this feature:

BLAST of IVF0020566 vs.


Match Name	E-value	Identity	Description

BLAST of IVF0020566 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3C176	9.9e-57	100.00	uncharacterized protein LOC103495319 OS=Cucumis melo OX=3656 GN=LOC103495319 PE=...	[more]
A0A6J1JNZ7	6.9e-42	79.26	uncharacterized protein LOC111486201 OS=Cucurbita maxima OX=3661 GN=LOC111486201...	[more]
A0A6J1D574	2.0e-41	78.91	uncharacterized protein LOC111017388 isoform X1 OS=Momordica charantia OX=3673 G...	[more]
A0A6J1EM63	7.6e-41	75.71	uncharacterized protein LOC111434615 OS=Cucurbita moschata OX=3662 GN=LOC1114346...	[more]
A0A2I4HLI8	6.9e-34	68.15	uncharacterized protein LOC109019213 OS=Juglans regia OX=51240 GN=LOC109019213 P...	[more]

BLAST of IVF0020566 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008455049.1	1.17e-74	100.00	PREDICTED: uncharacterized protein LOC103495319 [Cucumis melo]	[more]
XP_004136934.1	7.23e-67	93.85	uncharacterized protein LOC101214221 [Cucumis sativus] >KAE8645839.1 hypothetica...	[more]
XP_038887440.1	5.94e-60	85.07	uncharacterized protein LOC120077574 [Benincasa hispida]	[more]
XP_022989008.1	3.79e-55	79.26	uncharacterized protein LOC111486201 [Cucurbita maxima]	[more]
XP_022148828.1	1.31e-54	78.91	uncharacterized protein LOC111017388 isoform X1 [Momordica charantia]	[more]

BLAST of IVF0020566 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G49975.1	1.7e-32	63.43	INVOLVED IN: photosynthesis; LOCATED IN: photosystem I, chloroplast, thylakoid m...	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 69..89
None	No IPR available	PANTHER	PTHR36327	UNNAMED PRODUCT	coord: 13..128
IPR008796	Photosystem I reaction centre subunit N, chloroplastic	PFAM	PF05479	PsaN	coord: 27..92 e-value: 9.4E-7 score: 29.1

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
IVF0020566.1	IVF0020566.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0015979	photosynthesis
cellular_component	GO:0009507	chloroplast
cellular_component	GO:0009522	photosystem I