IVF0020427 (gene) Melon (IVF77) v1

Overview


Name	IVF0020427
Type	gene
Organism	Cucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
Description	tRNA pseudouridine synthase A
Location	chr07: 507123 .. 509718 (+)
RNA-Seq Expression	IVF0020427
Synteny	IVF0020427

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GAAAAACTATTATTGAGGGGAAGTTACTAAAATCTGTTGGATGGGATGGTCTCTAAAACCCTAATGTCTACTTCACTCCATTTCCCTACCCGACTAGAATGGAAGAAGAACCGCCAACTGAAACCAGAAGGTCACCGCCACAACCGTTGATCGAAGAACCCGACCCCAAGAAGCTCAAAATGTCATCTACAACCACCACATCCGACGACGAAGATGTCAATTCCAACTCTGCGGCGCCCAAGAAACAAAGGTACAAGCGCCGCAAGGTCGCCATTTTCTTTGCCTACTGTGGCGTCGGTTACCAAGGGATGCAGAAGAACCCTGGCGCTAAAACCATTGAAGCTGACCTTGAAGAGGCCCTATATCTCGCCGGAGCCGTCCCGGAGCAGGACCGAGGGCTCTCGAAGCGCTATGATTGGGCCCGCTCGGCTCGCACTGATAAGGGCGTGAGCGCCGTGGGTCAGGTCGTTTCTGGTCGCTTCTACATCGATCCACCAGGTTTTGTTGATCGTCTCAATTCGAATCTTTCGCCTCAGATTCGGATTTTCGGGTACAAGCGCGTCACAGCGTCATTCAATGCGAAAAAGTTCTGCGATCGGAGAAGGTATGTATACCTAATCCCTGTCTTCGCTCTCGATCCCAATTGCCATCGTGATAGAGAAAGTGTGAAAGCTAGTTTAGGGTCTGATAACGAGCTTGTGAAGTGCTTAGAGTGCTCTGAACGCGGACGCAAGGTCGAAGGCTTGATGGGCAAGCGCAATTTCGAAGTAAAAACTGCGATAACTGAGTCAGACATTTCGTCGAACATTGTCAACGCCATTGAAGTTTCTGATGTTCAAGCAAATGGGGTTTTGGAGGACAGTGCCACGACCTCTGATTCTAACAAACAAACTAGCGTTTTAGTAGAAAACACTGCTACTGAACTCACTGTTGCAAGCGAAGGTATTGCAAAAAGTGAAAGCAGTGAAATGTCTGGATTAGGGAATGATTCAGAGATTGTTGTAAGCAAAGAGAGCGTTAATGGGAAAGAGAAACCTTCAGGGAGCTTCAAGTACGGCGAGGAGGAAAAGGCTAGGTTCAATAGGATTTTAAAACATTATGTGGGAACTCATAACTTCCACAATTTTACTACTAGAACAAAAGCAGAGGATCCTGCTGCTCGCCGATTCATTGTTTCGTTTGATGCTTCTACAACCGTCGTCGTTGAAGGCATTGAATTTGTGAAATGTGAAGTTGTAGGGCAGAGCTTCATGCTTCATCAGATCCGTAAGATGATGGGTCTTGCTGTGGCAATCATGAGGAACTGTGCTCCAGAGTCATTGATCGAGAAAGCTTTGCAAAAGTAAGTAATTTCCATTTTCGACAACGATTTGAATTGATCCAATTTGATTTTCATTTGTTATTAGAATAGAATAGAATTGGATTTCACCAACTTCTGGTAATCAAAATTCAAACACAGGGATGTAAATATCAATGTGCCCACTGCACCTGAGGTTGGGCTGTACTTAGATGAGTGCCTATTCACATCATATAATCAAAAGTGGAAAGACACTCATGAAGAGGTATCAATGAAAGCATACGAAGAGGTAGCAGAAGATTTCAAAATGAAACAAATATATTCTCACATTGCTTCCACAGAGCACAAAGATGGAGCCGTGGCCTTATGGTTGCATTCTCTAAATCATCGAAATTATCCAGATTTACGTGTCATTGATGAAGTTGCCAAAATTGCTAAGAACACCAGTGTTGACATGGAGACGCAAGCTGAAACTTAAAAGGTCTTCTTCCACCTTTGCTTTTATTTAAGCATTAACCGTCCATTTGGATTCTGAGCGGAAAAATACTTGTTTTTGATGATTTAGTGCTTGTTAAATTATAAACCCAATCAGATTATGCATAAATTTTGCTTAAGAAAGTGAGGCAAATCCATTAATTATGTTAAGTTCTAATTACTACCTATGAGTCATATTTTCCTTATAGAACCCTACTGACAAGGGTTATAATTTTTCTAGAAAACATAGGTCAACGAGAGTTGTTGTAACTTTGAGAATATTAGATTAGGGGTACTAGAGAGGAACTCTAAAATGTTTTTTGCCCAATTCATGTAGAAAATCTGCTCTCGAGATTGTATATCGTAGATGACCTTCAGCTTATATAACTTTTGGAACATCTAATGAAGAATATCTGCAGCAGTTCATTGCCTTCAGCTTAGATTTATTTGAGGAAAAAAGAATGGTTTCTTTCTTGCACTTTCTTTTCAATTGGGTCGTGCTCGAGTTTGTGAATAACTCAAGAAGCAGTAGACCACAAAAATTTGGAGAGGTCAAACACTTTGGTGTTTGACATAAAATTCTCACCTGACCATTTAGCAAGGATTTCTGGAATGCTGTCTGGCAAATCAAATTAGACGTTCTGATAGTCGTTTGAGAAACAATAAAGAATTGTCACGAAACATGTAAAAGTTTTTAAAATATGTTTATGTATTTTGTAAGGAGCAACATGTAAGCACTTTATTTGTTGTCCTTTTTCTTTTGTTATCCATTTAATCCATTAGTTTTGAACCTCCCCGTACTAATTAAAGGATTCTATTTCCATC

mRNA sequence

GAAAAACTATTATTGAGGGGAAGTTACTAAAATCTGTTGGATGGGATGGTCTCTAAAACCCTAATGTCTACTTCACTCCATTTCCCTACCCGACTAGAATGGAAGAAGAACCGCCAACTGAAACCAGAAGGTCACCGCCACAACCGTTGATCGAAGAACCCGACCCCAAGAAGCTCAAAATGTCATCTACAACCACCACATCCGACGACGAAGATGTCAATTCCAACTCTGCGGCGCCCAAGAAACAAAGGTACAAGCGCCGCAAGGTCGCCATTTTCTTTGCCTACTGTGGCGTCGGTTACCAAGGGATGCAGAAGAACCCTGGCGCTAAAACCATTGAAGCTGACCTTGAAGAGGCCCTATATCTCGCCGGAGCCGTCCCGGAGCAGGACCGAGGGCTCTCGAAGCGCTATGATTGGGCCCGCTCGGCTCGCACTGATAAGGGCGTGAGCGCCGTGGGTCAGGTCGTTTCTGGTCGCTTCTACATCGATCCACCAGGTTTTGTTGATCGTCTCAATTCGAATCTTTCGCCTCAGATTCGGATTTTCGGGTACAAGCGCGTCACAGCGTCATTCAATGCGAAAAAGTTCTGCGATCGGAGAAGGTATGTATACCTAATCCCTGTCTTCGCTCTCGATCCCAATTGCCATCGTGATAGAGAAAGTGTGAAAGCTAGTTTAGGGTCTGATAACGAGCTTGTGAAGTGCTTAGAGTGCTCTGAACGCGGACGCAAGGTCGAAGGCTTGATGGGCAAGCGCAATTTCGAAGTAAAAACTGCGATAACTGAGTCAGACATTTCGTCGAACATTGTCAACGCCATTGAAGTTTCTGATGTTCAAGCAAATGGGGTTTTGGAGGACAGTGCCACGACCTCTGATTCTAACAAACAAACTAGCGTTTTAGTAGAAAACACTGCTACTGAACTCACTGTTGCAAGCGAAGGTATTGCAAAAAGTGAAAGCAGTGAAATGTCTGGATTAGGGAATGATTCAGAGATTGTTGTAAGCAAAGAGAGCGTTAATGGGAAAGAGAAACCTTCAGGGAGCTTCAAGTACGGCGAGGAGGAAAAGGCTAGGTTCAATAGGATTTTAAAACATTATGTGGGAACTCATAACTTCCACAATTTTACTACTAGAACAAAAGCAGAGGATCCTGCTGCTCGCCGATTCATTGTTTCGTTTGATGCTTCTACAACCGTCGTCGTTGAAGGCATTGAATTTGTGAAATGTGAAGTTGTAGGGCAGAGCTTCATGCTTCATCAGATCCGTAAGATGATGGGTCTTGCTGTGGCAATCATGAGGAACTGTGCTCCAGAGTCATTGATCGAGAAAGCTTTGCAAAAGGATGTAAATATCAATGTGCCCACTGCACCTGAGGTTGGGCTGTACTTAGATGAGTGCCTATTCACATCATATAATCAAAAGTGGAAAGACACTCATGAAGAGGTATCAATGAAAGCATACGAAGAGGTAGCAGAAGATTTCAAAATGAAACAAATATATTCTCACATTGCTTCCACAGAGCACAAAGATGGAGCCGTGGCCTTATGGTTGCATTCTCTAAATCATCGAAATTATCCAGATTTACGTGTCATTGATGAAGTTGCCAAAATTGCTAAGAACACCAGTGTTGACATGGAGACGCAAGCTGAAACTTAAAAGGTCTTCTTCCACCTTTGCTTTTATTTAAGCATTAACCGTCCATTTGGATTCTGAGCGGAAAAATACTTGTTTTTGATGATTTAGTGCTTGTTAAATTATAAACCCAATCAGATTATGCATAAATTTTGCTTAAGAAAGTGAGGCAAATCCATTAATTATGTTAAGTTCTAATTACTACCTATGAGTCATATTTTCCTTATAGAACCCTACTGACAAGGGTTATAATTTTTCTAGAAAACATAGGTCAACGAGAGTTGTTGTAACTTTGAGAATATTAGATTAGGGGTACTAGAGAGGAACTCTAAAATGTTTTTTGCCCAATTCATGTAGAAAATCTGCTCTCGAGATTGTATATCGTAGATGACCTTCAGCTTATATAACTTTTGGAACATCTAATGAAGAATATCTGCAGCAGTTCATTGCCTTCAGCTTAGATTTATTTGAGGAAAAAAGAATGGTTTCTTTCTTGCACTTTCTTTTCAATTGGGTCGTGCTCGAGTTTGTGAATAACTCAAGAAGCAGTAGACCACAAAAATTTGGAGAGGTCAAACACTTTGGTGTTTGACATAAAATTCTCACCTGACCATTTAGCAAGGATTTCTGGAATGCTGTCTGGCAAATCAAATTAGACGTTCTGATAGTCGTTTGAGAAACAATAAAGAATTGTCACGAAACATGTAAAAGTTTTTAAAATATGTTTATGTATTTTGTAAGGAGCAACATGTAAGCACTTTATTTGTTGTCCTTTTTCTTTTGTTATCCATTTAATCCATTAGTTTTGAACCTCCCCGTACTAATTAAAGGATTCTATTTCCATC

Coding sequence (CDS)

ATGGAAGAAGAACCGCCAACTGAAACCAGAAGGTCACCGCCACAACCGTTGATCGAAGAACCCGACCCCAAGAAGCTCAAAATGTCATCTACAACCACCACATCCGACGACGAAGATGTCAATTCCAACTCTGCGGCGCCCAAGAAACAAAGGTACAAGCGCCGCAAGGTCGCCATTTTCTTTGCCTACTGTGGCGTCGGTTACCAAGGGATGCAGAAGAACCCTGGCGCTAAAACCATTGAAGCTGACCTTGAAGAGGCCCTATATCTCGCCGGAGCCGTCCCGGAGCAGGACCGAGGGCTCTCGAAGCGCTATGATTGGGCCCGCTCGGCTCGCACTGATAAGGGCGTGAGCGCCGTGGGTCAGGTCGTTTCTGGTCGCTTCTACATCGATCCACCAGGTTTTGTTGATCGTCTCAATTCGAATCTTTCGCCTCAGATTCGGATTTTCGGGTACAAGCGCGTCACAGCGTCATTCAATGCGAAAAAGTTCTGCGATCGGAGAAGGTATGTATACCTAATCCCTGTCTTCGCTCTCGATCCCAATTGCCATCGTGATAGAGAAAGTGTGAAAGCTAGTTTAGGGTCTGATAACGAGCTTGTGAAGTGCTTAGAGTGCTCTGAACGCGGACGCAAGGTCGAAGGCTTGATGGGCAAGCGCAATTTCGAAGTAAAAACTGCGATAACTGAGTCAGACATTTCGTCGAACATTGTCAACGCCATTGAAGTTTCTGATGTTCAAGCAAATGGGGTTTTGGAGGACAGTGCCACGACCTCTGATTCTAACAAACAAACTAGCGTTTTAGTAGAAAACACTGCTACTGAACTCACTGTTGCAAGCGAAGGTATTGCAAAAAGTGAAAGCAGTGAAATGTCTGGATTAGGGAATGATTCAGAGATTGTTGTAAGCAAAGAGAGCGTTAATGGGAAAGAGAAACCTTCAGGGAGCTTCAAGTACGGCGAGGAGGAAAAGGCTAGGTTCAATAGGATTTTAAAACATTATGTGGGAACTCATAACTTCCACAATTTTACTACTAGAACAAAAGCAGAGGATCCTGCTGCTCGCCGATTCATTGTTTCGTTTGATGCTTCTACAACCGTCGTCGTTGAAGGCATTGAATTTGTGAAATGTGAAGTTGTAGGGCAGAGCTTCATGCTTCATCAGATCCGTAAGATGATGGGTCTTGCTGTGGCAATCATGAGGAACTGTGCTCCAGAGTCATTGATCGAGAAAGCTTTGCAAAAGGATGTAAATATCAATGTGCCCACTGCACCTGAGGTTGGGCTGTACTTAGATGAGTGCCTATTCACATCATATAATCAAAAGTGGAAAGACACTCATGAAGAGGTATCAATGAAAGCATACGAAGAGGTAGCAGAAGATTTCAAAATGAAACAAATATATTCTCACATTGCTTCCACAGAGCACAAAGATGGAGCCGTGGCCTTATGGTTGCATTCTCTAAATCATCGAAATTATCCAGATTTACGTGTCATTGATGAAGTTGCCAAAATTGCTAAGAACACCAGTGTTGACATGGAGACGCAAGCTGAAACTTAA

Protein sequence

MEEEPPTETRRSPPQPLIEEPDPKKLKMSSTTTTSDDEDVNSNSAAPKKQRYKRRKVAIFFAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAVGQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALDPNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKTAITESDISSNIVNAIEVSDVQANGVLEDSATTSDSNKQTSVLVENTATELTVASEGIAKSESSEMSGLGNDSEIVVSKESVNGKEKPSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFIVSFDASTTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNINVPTAPEVGLYLDECLFTSYNQKWKDTHEEVSMKAYEEVAEDFKMKQIYSHIASTEHKDGAVALWLHSLNHRNYPDLRVIDEVAKIAKNTSVDMETQAET

Homology

[Close]

BLAST of IVF0020427 vs. ExPASy Swiss-Prot
Match: Q59S63 (tRNA pseudouridine synthase 1 OS=Candida albicans (strain SC5314 / ATCC MYA-2876) OX=237561 GN=PUS1 PE=3 SV=2)

HSP 1 Score: 212.2 bits (539), Expect = 1.4e-53
Identity = 161/484 (33.26%), Postives = 237/484 (48.97%), Query Frame = 0


Query: 4   EPPTETRRSPPQPLIEEPDPKKLK------MSSTTTTSDDEDVNSNSAAPKKQRYKRRKV 63
           E  T  R+S P P   E D K+LK           T   DE   +    PK  R  +RKV
Sbjct: 95  ESTTRVRKSSPSPSANENDKKRLKKEFQPRQKIEYTPLVDE---NGQPIPKAPRKPKRKV 154

Query: 64  AIFFAYCGVGYQGM--QKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDK 123
           A+   YCG GY GM  Q +P  KTIE D+ +A+  AGA+  ++    K+  + R+ARTDK
Sbjct: 155 AVMLGYCGTGYNGMQVQNDPNVKTIEKDIYDAMATAGAISAENAVDLKKSGFQRAARTDK 214

Query: 124 GVSAVGQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIP 183
           GV A G V+S +  I+ P  ++++N  L  QIRI+G +R T  F+ +K C  R Y YL+P
Sbjct: 215 GVHAAGNVISLKMIIEDPEIINKINDLLPKQIRIWGIQRTTKGFDCRKCCSSRIYEYLLP 274

Query: 184 VFALDPNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKTAITESDISS 243
            F+L P      +SV + L  + +L +  +  E  +  EG+    N  VK+ I  S I+ 
Sbjct: 275 TFSLLP---PKPKSVLSELVKEKKL-ENPDLFEDDQ--EGIDWWEN--VKSKILASGITQ 334

Query: 244 NIVNAIEVSDVQANGVLEDSATTSDSNKQTSVLVENTATELTVASEGIAKSESSEMSGLG 303
             +               DS T+S   +Q          +     +  A  E  ++S L 
Sbjct: 335 EQI---------------DSITSSYDEQQ---------QQQQQQQQQQADEEERDISELS 394

Query: 304 NDSEIVVSKESVNGKEKPSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAAR 363
               I   K   N   +   S++        F  ++K Y GTHNFHNFT     +D +A 
Sbjct: 395 FTKLIKQIKTIENQSRR---SYRISSSRLQHFREVMKQYEGTHNFHNFTVGKPFKDTSAN 454

Query: 364 RFIVSFDASTTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQK 423
           RF++    S   V+EG E++  ++ GQSFMLHQIRKM+ +A  ++R   P  +I    Q 
Sbjct: 455 RFMIKTIVSDPFVIEGTEWISIKIHGQSFMLHQIRKMIAMAALVVRLSLPCGIINNFFQ- 514

Query: 424 DVNINVPTAPEVGLYLDECLFTSYNQK-WKDTHEEVSMKAYEEVAEDFKMKQIYSHIAST 479
              IN+P AP +GL L+  +F  YN K  K  +E +    +++   +FKMK IY  I + 
Sbjct: 515 STKINIPKAPALGLLLENPVFDGYNIKLTKSDYEPIDFTKFDKEMNEFKMKYIYDKIYAE 539

[Close]

BLAST of IVF0020427 vs. ExPASy Swiss-Prot
Match: Q6CC39 (tRNA pseudouridine synthase 1 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) OX=284591 GN=PUS1 PE=3 SV=1)

HSP 1 Score: 199.1 bits (505), Expect = 1.2e-49
Identity = 138/431 (32.02%), Postives = 211/431 (48.96%), Query Frame = 0


Query: 51  RYKRRKVAIFFAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARS 110
           R  +RKVA    YCG GY GMQ NP  KTIE D+ +A   AGA+ + +    K+  + R+
Sbjct: 81  RQPKRKVACMIGYCGTGYHGMQLNPPQKTIEGDIFQAFVKAGAISQNNADDPKKSAFMRA 140

Query: 111 ARTDKGVSAVGQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRY 170
           ARTDKGV A G V+S +  I+    V+++NS+L  Q+R++G  R   +F  +K C  R Y
Sbjct: 141 ARTDKGVHAAGNVISLKMIIEDENIVEKINSHLPEQLRVWGVSRTNKAFECRKLCSSRVY 200

Query: 171 VYLIPVFAL---DPNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKTA 230
            YL+P ++     P      + +K     D E                  GK+ +E   A
Sbjct: 201 EYLMPTYSFLNPRPGTVMSEKLLKDGTSPDEE------------------GKKYWESVAA 260

Query: 231 ITESDISSNIVNAIEVSDVQANGVLEDSATTSDSNKQTSVLVENTATELTVASEGIAKSE 290
                            D+++ GV      + D   + + + E    E    +E   K++
Sbjct: 261 -----------------DLESQGV------SYDEWMKRACIDEIKGEETKEVAESEVKTD 320

Query: 291 SSEMSGLGNDSEIVVSKESVNGKEKPSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRT 350
           S        D+  +   ++V  + +    F+   E  A+   ILK Y GTHNFHNFT   
Sbjct: 321 SK------TDAATLEKIKAVERRHRE--EFRISGERLAKIREILKIYEGTHNFHNFTLGK 380

Query: 351 KAEDPAARRFIVSFDASTTVVVEGIEFVKCEVVGQSFMLHQIRKMMGL-AVAIMRNCAPE 410
             +DP+A R + S   S   +++G E+V  ++ GQSFMLHQIRKM+ + A+++  N  P+
Sbjct: 381 AFKDPSAMRTMKSLTCSDPFLIDGTEWVSIKIHGQSFMLHQIRKMISMVALSVRCNADPQ 440

Query: 411 SLIEKALQKDVNINVPTAPEVGLYLDECLFTSYNQKWKDT--HEEVSMKAYEEVAEDFKM 470
            LI +  +K   IN+P AP +GL L+  ++ SYN+K +     E V    + +  E FK 
Sbjct: 441 KLIPQTFEK-ARINIPKAPALGLLLERPVYDSYNKKLQGEFGREGVHFDNWNDQIEAFKH 461

Query: 471 KQIYSHIASTE 476
           K IY  I + E
Sbjct: 501 KFIYDKIYAEE 461

[Close]

BLAST of IVF0020427 vs. ExPASy Swiss-Prot
Match: Q9Y606 (tRNA pseudouridine synthase A OS=Homo sapiens OX=9606 GN=PUS1 PE=1 SV=3)

HSP 1 Score: 196.4 bits (498), Expect = 7.8e-49
Identity = 140/459 (30.50%), Postives = 201/459 (43.79%), Query Frame = 0


Query: 40  VNSNSAAPKKQRYKRRKVAIFFAYCGVGYQGMQKNPGA---KTIEADLEEALYLAGAVPE 99
           + S     ++++  +RK+ +  AY G GY GMQ+N G+   KTIE DL  AL  +G +PE
Sbjct: 69  LKSGGDEERREKPPKRKIVLLMAYSGKGYHGMQRNVGSSQFKTIEDDLVSALVRSGCIPE 128

Query: 100 QDRGLSKRYDWARSARTDKGVSAVGQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVT 159
                 ++  + R ARTDKGVSA GQVVS + ++     ++++NS+L   IRI G KRVT
Sbjct: 129 NHGEDMRKMSFQRCARTDKGVSAAGQVVSLKVWL-IDDILEKINSHLPSHIRILGLKRVT 188

Query: 160 ASFNAKKFCDRRRYVYLIPVFALDPNCHRDRESVKASLGSDNELVKCLECSERGRKVEGL 219
             FN+K  CD R Y YL+P FA     H+DR                             
Sbjct: 189 GGFNSKNRCDARTYCYLLPTFAF---AHKDR----------------------------- 248

Query: 220 MGKRNFEVKTAITESDISSNIVNAIEVSDVQANGVLEDSATTSDSNKQTSVLVENTATEL 279
                                       DVQ                             
Sbjct: 249 ----------------------------DVQ----------------------------- 308

Query: 280 TVASEGIAKSESSEMSGLGNDSEIVVSKESVNGKEKPSGSFKYGEEEKARFNRILKHYVG 339
                                                  +++   E   + NR+L  Y G
Sbjct: 309 -------------------------------------DETYRLSAETLQQVNRLLACYKG 368

Query: 340 THNFHNFTTRTKAEDPAARRFIVSFDASTTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLA 399
           THNFHNFT++   +DP+A R+I+        V EG+EF    V GQSFM+HQIRKM+GL 
Sbjct: 369 THNFHNFTSQKGPQDPSACRYILEMYCEEPFVREGLEFAVIRVKGQSFMMHQIRKMVGLV 399

Query: 400 VAIMRNCAPESLIEKALQKDVNINVPTAPEVGLYLDECLFTSYNQKWKD--THEEVSMKA 459
           VAI++  APES++E++   +  ++VP AP +GL L+   F  YNQ++ +   HE +    
Sbjct: 429 VAIVKGYAPESVLERSWGTE-KVDVPKAPGLGLVLERVHFEKYNQRFGNDGLHEPLDWAQ 399

Query: 460 YEEVAEDFKMKQIYSHIASTEHKDGAVALWLHSLNHRNY 494
            E     FK + IY  I  TE  + ++A WL +L   N+
Sbjct: 489 EEGKVAAFKEEHIYPTIIGTERDERSMAQWLSTLPIHNF 399

[Close]

BLAST of IVF0020427 vs. ExPASy Swiss-Prot
Match: Q9WU56 (tRNA pseudouridine synthase A OS=Mus musculus OX=10090 GN=Pus1 PE=1 SV=2)

HSP 1 Score: 195.3 bits (495), Expect = 1.7e-48
Identity = 150/495 (30.30%), Postives = 213/495 (43.03%), Query Frame = 0


Query: 5   PPTETRRSPPQPLIEEPDPK-KLKMSSTTTTSDDEDVNSNSAAPKKQRYKRRKVAIFFAY 64
           PP    + PP     +PD K +       T    + V         ++  +RK+ +  AY
Sbjct: 29  PPMAGNKVPPALASHQPDRKGRGGWVWEETEHPAKRVKGGEDEEPPRKLPKRKIVLLMAY 88

Query: 65  CGVGYQGMQKNPGA---KTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV 124
            G GY GMQ+N G+   +TIE DL  AL  AG +PE      ++  + R ARTDKGVSA 
Sbjct: 89  SGKGYHGMQRNLGSSQFRTIEDDLVSALVQAGCIPENHGTDMRKMSFQRCARTDKGVSAA 148

Query: 125 GQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD 184
           GQVVS + ++     +D++NS+L   IRI G KRVT  FN+K  CD R Y Y++P FA  
Sbjct: 149 GQVVSLKVWL-IDDILDKINSHLPSHIRILGLKRVTGGFNSKNKCDARTYCYMLPTFAF- 208

Query: 185 PNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKTAITESDISSNIVNA 244
              H+DR                                                     
Sbjct: 209 --AHKDR----------------------------------------------------- 268

Query: 245 IEVSDVQANGVLEDSATTSDSNKQTSVLVENTATELTVASEGIAKSESSEMSGLGNDSEI 304
               DVQ                                                     
Sbjct: 269 ----DVQ----------------------------------------------------- 328

Query: 305 VVSKESVNGKEKPSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFIVS 364
                          S++   E   + NR+L  Y GTHNFHNFT++    +P+ARR+I+ 
Sbjct: 329 -------------DESYRLSAETLQQVNRLLACYKGTHNFHNFTSQKGPREPSARRYILE 388

Query: 365 FDASTTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNIN 424
                  V EG+EF   +V GQSFM+HQIRKM+GL VAI++  APES++E++  ++  ++
Sbjct: 389 MYCEEPFVREGLEFAVIKVKGQSFMMHQIRKMVGLVVAIVKGYAPESVLERSWGEE-KVD 395

Query: 425 VPTAPEVGLYLDECLFTSYNQKW--KDTHEEVSMKAYEEVAEDFKMKQIYSHIASTEHKD 484
           VP AP +GL L+   F  YNQ++     HE +     E     FK + IY  I STE  +
Sbjct: 449 VPKAPGLGLVLERVHFEKYNQRFGGDGLHEPLDWTQEEGKVTAFKEQYIYPTIVSTERDE 395

Query: 485 GAVALWLHSLNHRNY 494
            ++A WL++L   N+
Sbjct: 509 RSMAQWLNTLPIHNF 395

[Close]

BLAST of IVF0020427 vs. ExPASy Swiss-Prot
Match: Q4KM92 (tRNA pseudouridine synthase A OS=Rattus norvegicus OX=10116 GN=Pus1 PE=1 SV=2)

HSP 1 Score: 194.1 bits (492), Expect = 3.9e-48
Identity = 150/495 (30.30%), Postives = 213/495 (43.03%), Query Frame = 0


Query: 5   PPTETRRSPPQPLIEEPDPK-KLKMSSTTTTSDDEDVNSNSAAPKKQRYKRRKVAIFFAY 64
           PP    + PP     +PD K +       T    + V         ++  +RK+ +  AY
Sbjct: 29  PPMAGNKVPPALPSHQPDRKGRGGWVWEETEHPAKRVKGGEDEEPPRKLPKRKIVLLMAY 88

Query: 65  CGVGYQGMQKNPGA---KTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV 124
            G GY GMQ+N G+   +TIE DL  AL  AG +PE      ++  + R ARTDKGVSA 
Sbjct: 89  SGKGYHGMQRNLGSSQFRTIEDDLVSALVQAGCIPENHGTDMRKMSFQRCARTDKGVSAA 148

Query: 125 GQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD 184
           GQVVS + ++     +D++NS+L   IRI G KRVT  FN+K  CD R Y Y++P FA  
Sbjct: 149 GQVVSLKVWL-IDDILDKINSHLPSHIRILGLKRVTGGFNSKNKCDARTYCYMLPTFAF- 208

Query: 185 PNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKTAITESDISSNIVNA 244
              H+DR                                                     
Sbjct: 209 --AHKDR----------------------------------------------------- 268

Query: 245 IEVSDVQANGVLEDSATTSDSNKQTSVLVENTATELTVASEGIAKSESSEMSGLGNDSEI 304
               DVQ                                                     
Sbjct: 269 ----DVQ----------------------------------------------------- 328

Query: 305 VVSKESVNGKEKPSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFIVS 364
                          S++   E   + NR+L  Y GTHNFHNFT++    +P+ARR+I+ 
Sbjct: 329 -------------DESYRLSAETLQQVNRLLGCYKGTHNFHNFTSQKGPREPSARRYILE 388

Query: 365 FDASTTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNIN 424
                  V EG+EF   +V GQSFM+HQIRKM+GL VAI++  APES++E++  ++  ++
Sbjct: 389 MYCEEPFVREGLEFAVIKVKGQSFMMHQIRKMVGLVVAIVKGYAPESVLERSWGEE-KVD 395

Query: 425 VPTAPEVGLYLDECLFTSYNQKW--KDTHEEVSMKAYEEVAEDFKMKQIYSHIASTEHKD 484
           VP AP +GL L+   F  YNQ++     HE +     E     FK + IY  I STE  +
Sbjct: 449 VPKAPGLGLVLERVHFEKYNQRFGSDGLHEPLDWAQEEGKVTAFKEQYIYPTIVSTERDE 395

Query: 485 GAVALWLHSLNHRNY 494
            ++A WL++L   N+
Sbjct: 509 RSMAQWLNTLPIHNF 395

[Close]

BLAST of IVF0020427 vs. ExPASy TrEMBL
Match: A0A1S3BV07 (tRNA pseudouridine synthase A, mitochondrial-like OS=Cucumis melo OX=3656 GN=LOC103493789 PE=3 SV=1)

HSP 1 Score: 1012.3 bits (2616), Expect = 7.3e-292
Identity = 519/519 (100.00%), Postives = 519/519 (100.00%), Query Frame = 0


Query: 1   MEEEPPTETRRSPPQPLIEEPDPKKLKMSSTTTTSDDEDVNSNSAAPKKQRYKRRKVAIF 60
           MEEEPPTETRRSPPQPLIEEPDPKKLKMSSTTTTSDDEDVNSNSAAPKKQRYKRRKVAIF
Sbjct: 1   MEEEPPTETRRSPPQPLIEEPDPKKLKMSSTTTTSDDEDVNSNSAAPKKQRYKRRKVAIF 60

Query: 61  FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV 120
           FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV
Sbjct: 61  FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV 120

Query: 121 GQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD 180
           GQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD
Sbjct: 121 GQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD 180

Query: 181 PNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKTAITESDISSNIVNA 240
           PNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKTAITESDISSNIVNA
Sbjct: 181 PNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKTAITESDISSNIVNA 240

Query: 241 IEVSDVQANGVLEDSATTSDSNKQTSVLVENTATELTVASEGIAKSESSEMSGLGNDSEI 300
           IEVSDVQANGVLEDSATTSDSNKQTSVLVENTATELTVASEGIAKSESSEMSGLGNDSEI
Sbjct: 241 IEVSDVQANGVLEDSATTSDSNKQTSVLVENTATELTVASEGIAKSESSEMSGLGNDSEI 300

Query: 301 VVSKESVNGKEKPSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFIVS 360
           VVSKESVNGKEKPSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFIVS
Sbjct: 301 VVSKESVNGKEKPSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFIVS 360

Query: 361 FDASTTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNIN 420
           FDASTTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNIN
Sbjct: 361 FDASTTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNIN 420

Query: 421 VPTAPEVGLYLDECLFTSYNQKWKDTHEEVSMKAYEEVAEDFKMKQIYSHIASTEHKDGA 480
           VPTAPEVGLYLDECLFTSYNQKWKDTHEEVSMKAYEEVAEDFKMKQIYSHIASTEHKDGA
Sbjct: 421 VPTAPEVGLYLDECLFTSYNQKWKDTHEEVSMKAYEEVAEDFKMKQIYSHIASTEHKDGA 480

Query: 481 VALWLHSLNHRNYPDLRVIDEVAKIAKNTSVDMETQAET 520
           VALWLHSLNHRNYPDLRVIDEVAKIAKNTSVDMETQAET
Sbjct: 481 VALWLHSLNHRNYPDLRVIDEVAKIAKNTSVDMETQAET 519

[Close]

BLAST of IVF0020427 vs. ExPASy TrEMBL
Match: A0A5A7VBT0 (tRNA pseudouridine synthase A OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G00860 PE=3 SV=1)

HSP 1 Score: 1012.3 bits (2616), Expect = 7.3e-292
Identity = 519/519 (100.00%), Postives = 519/519 (100.00%), Query Frame = 0


Query: 1   MEEEPPTETRRSPPQPLIEEPDPKKLKMSSTTTTSDDEDVNSNSAAPKKQRYKRRKVAIF 60
           MEEEPPTETRRSPPQPLIEEPDPKKLKMSSTTTTSDDEDVNSNSAAPKKQRYKRRKVAIF
Sbjct: 1   MEEEPPTETRRSPPQPLIEEPDPKKLKMSSTTTTSDDEDVNSNSAAPKKQRYKRRKVAIF 60

Query: 61  FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV 120
           FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV
Sbjct: 61  FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV 120

Query: 121 GQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD 180
           GQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD
Sbjct: 121 GQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD 180

Query: 181 PNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKTAITESDISSNIVNA 240
           PNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKTAITESDISSNIVNA
Sbjct: 181 PNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKTAITESDISSNIVNA 240

Query: 241 IEVSDVQANGVLEDSATTSDSNKQTSVLVENTATELTVASEGIAKSESSEMSGLGNDSEI 300
           IEVSDVQANGVLEDSATTSDSNKQTSVLVENTATELTVASEGIAKSESSEMSGLGNDSEI
Sbjct: 241 IEVSDVQANGVLEDSATTSDSNKQTSVLVENTATELTVASEGIAKSESSEMSGLGNDSEI 300

Query: 301 VVSKESVNGKEKPSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFIVS 360
           VVSKESVNGKEKPSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFIVS
Sbjct: 301 VVSKESVNGKEKPSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFIVS 360

Query: 361 FDASTTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNIN 420
           FDASTTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNIN
Sbjct: 361 FDASTTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNIN 420

Query: 421 VPTAPEVGLYLDECLFTSYNQKWKDTHEEVSMKAYEEVAEDFKMKQIYSHIASTEHKDGA 480
           VPTAPEVGLYLDECLFTSYNQKWKDTHEEVSMKAYEEVAEDFKMKQIYSHIASTEHKDGA
Sbjct: 421 VPTAPEVGLYLDECLFTSYNQKWKDTHEEVSMKAYEEVAEDFKMKQIYSHIASTEHKDGA 480

Query: 481 VALWLHSLNHRNYPDLRVIDEVAKIAKNTSVDMETQAET 520
           VALWLHSLNHRNYPDLRVIDEVAKIAKNTSVDMETQAET
Sbjct: 481 VALWLHSLNHRNYPDLRVIDEVAKIAKNTSVDMETQAET 519

[Close]

BLAST of IVF0020427 vs. ExPASy TrEMBL
Match: E5GCD2 (tRNA pseudouridine synthase family protein OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1)

HSP 1 Score: 1012.3 bits (2616), Expect = 7.3e-292
Identity = 519/519 (100.00%), Postives = 519/519 (100.00%), Query Frame = 0


Query: 1   MEEEPPTETRRSPPQPLIEEPDPKKLKMSSTTTTSDDEDVNSNSAAPKKQRYKRRKVAIF 60
           MEEEPPTETRRSPPQPLIEEPDPKKLKMSSTTTTSDDEDVNSNSAAPKKQRYKRRKVAIF
Sbjct: 1   MEEEPPTETRRSPPQPLIEEPDPKKLKMSSTTTTSDDEDVNSNSAAPKKQRYKRRKVAIF 60

Query: 61  FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV 120
           FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV
Sbjct: 61  FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV 120

Query: 121 GQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD 180
           GQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD
Sbjct: 121 GQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD 180

Query: 181 PNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKTAITESDISSNIVNA 240
           PNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKTAITESDISSNIVNA
Sbjct: 181 PNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKTAITESDISSNIVNA 240

Query: 241 IEVSDVQANGVLEDSATTSDSNKQTSVLVENTATELTVASEGIAKSESSEMSGLGNDSEI 300
           IEVSDVQANGVLEDSATTSDSNKQTSVLVENTATELTVASEGIAKSESSEMSGLGNDSEI
Sbjct: 241 IEVSDVQANGVLEDSATTSDSNKQTSVLVENTATELTVASEGIAKSESSEMSGLGNDSEI 300

Query: 301 VVSKESVNGKEKPSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFIVS 360
           VVSKESVNGKEKPSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFIVS
Sbjct: 301 VVSKESVNGKEKPSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFIVS 360

Query: 361 FDASTTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNIN 420
           FDASTTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNIN
Sbjct: 361 FDASTTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNIN 420

Query: 421 VPTAPEVGLYLDECLFTSYNQKWKDTHEEVSMKAYEEVAEDFKMKQIYSHIASTEHKDGA 480
           VPTAPEVGLYLDECLFTSYNQKWKDTHEEVSMKAYEEVAEDFKMKQIYSHIASTEHKDGA
Sbjct: 421 VPTAPEVGLYLDECLFTSYNQKWKDTHEEVSMKAYEEVAEDFKMKQIYSHIASTEHKDGA 480

Query: 481 VALWLHSLNHRNYPDLRVIDEVAKIAKNTSVDMETQAET 520
           VALWLHSLNHRNYPDLRVIDEVAKIAKNTSVDMETQAET
Sbjct: 481 VALWLHSLNHRNYPDLRVIDEVAKIAKNTSVDMETQAET 519

[Close]

BLAST of IVF0020427 vs. ExPASy TrEMBL
Match: A0A0A0L0J9 (PseudoU_synth_1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G652870 PE=3 SV=1)

HSP 1 Score: 987.3 bits (2551), Expect = 2.5e-284
Identity = 506/519 (97.50%), Postives = 509/519 (98.07%), Query Frame = 0


Query: 1   MEEEPPTETRRSPPQPLIEEPDPKKLKMSSTTTTSDDEDVNSNSAAPKKQRYKRRKVAIF 60
           MEEEPPTETRRSPPQPLIEEP PKKLKMSSTTTTSDDEDVNSNSAAPKKQRYKRRKVAIF
Sbjct: 1   MEEEPPTETRRSPPQPLIEEPQPKKLKMSSTTTTSDDEDVNSNSAAPKKQRYKRRKVAIF 60

Query: 61  FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV 120
           FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV
Sbjct: 61  FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV 120

Query: 121 GQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD 180
           GQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD
Sbjct: 121 GQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD 180

Query: 181 PNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKTAITESDISSNIVNA 240
           PNCHRDRESVKASLGSDNELVKCLECSERGRKVEGL+GKRNFEVKTAITESDISSN VNA
Sbjct: 181 PNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLIGKRNFEVKTAITESDISSNTVNA 240

Query: 241 IEVSDVQANGVLEDSATTSDSNKQTSVLVENTATELTVASEGIAKSESSEMSGLGNDSEI 300
           IEVSDVQANGVLEDSATTSDSNKQTSVLVENTATEL V SEG AKSESSEMSGLG+DSEI
Sbjct: 241 IEVSDVQANGVLEDSATTSDSNKQTSVLVENTATELNVGSEGTAKSESSEMSGLGSDSEI 300

Query: 301 VVSKESVNGKEKPSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFIVS 360
           VVSKESVNGKEK SGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFIVS
Sbjct: 301 VVSKESVNGKEKSSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFIVS 360

Query: 361 FDASTTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNIN 420
           FDAS  VVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNIN
Sbjct: 361 FDASAIVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNIN 420

Query: 421 VPTAPEVGLYLDECLFTSYNQKWKDTHEEVSMKAYEEVAEDFKMKQIYSHIASTEHKDGA 480
           VPTAPEVGLYLDECLFTSYNQKWKDTHEEVSMKAYEE AEDFKMKQIYSHIASTEHKDGA
Sbjct: 421 VPTAPEVGLYLDECLFTSYNQKWKDTHEEVSMKAYEEAAEDFKMKQIYSHIASTEHKDGA 480

Query: 481 VALWLHSLNHRNYPDLRVIDEVAKIAKNTSVDMETQAET 520
           VALWLHSLNHRNYPDLRVIDEVAKIAKN S+DMETQAET
Sbjct: 481 VALWLHSLNHRNYPDLRVIDEVAKIAKNASLDMETQAET 519

[Close]

BLAST of IVF0020427 vs. ExPASy TrEMBL
Match: A0A6J1IJ76 (tRNA pseudouridine synthase A OS=Cucurbita maxima OX=3661 GN=LOC111474781 PE=3 SV=1)

HSP 1 Score: 942.2 bits (2434), Expect = 9.3e-271
Identity = 480/519 (92.49%), Postives = 495/519 (95.38%), Query Frame = 0


Query: 1   MEEEPPTETRRSPPQPLIEEPDPKKLKMSSTTTTSDDEDVNSNSAAPKKQRYKRRKVAIF 60
           MEEEPPT+TRRSPPQP IEEP+PKKLKMSSTTTTSDDEDVNSNSAAPKKQRYKRRK+AIF
Sbjct: 1   MEEEPPTQTRRSPPQPSIEEPEPKKLKMSSTTTTSDDEDVNSNSAAPKKQRYKRRKIAIF 60

Query: 61  FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV 120
           FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV
Sbjct: 61  FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV 120

Query: 121 GQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD 180
           GQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD
Sbjct: 121 GQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD 180

Query: 181 PNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKTAITESDISSNIVNA 240
           PNCHRDRESVKASLGS NELVKCLECSERGRKVEGLMGKRNFEVKTAI+ESDIS N  NA
Sbjct: 181 PNCHRDRESVKASLGSGNELVKCLECSERGRKVEGLMGKRNFEVKTAISESDISPNFGNA 240

Query: 241 IEVSDVQANGVLEDSATTSDSNKQTSVLVENTATELTVASEGIAKSESSEMSGLGNDSEI 300
           IEVSDVQANG LED+AT SDS K+TSVLVEN+ TEL V SE IAKSE SEMSGLGNDSE 
Sbjct: 241 IEVSDVQANGALEDNATASDSIKETSVLVENSGTELNVGSEAIAKSEGSEMSGLGNDSEN 300

Query: 301 VVSKESVNGKEKPSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFIVS 360
           VVSK+SVN  EKPSGSFKYGEEEKARFNRILK YVGTHNFHNFTTRTKAEDPAARRFI+S
Sbjct: 301 VVSKDSVNRNEKPSGSFKYGEEEKARFNRILKKYVGTHNFHNFTTRTKAEDPAARRFIIS 360

Query: 361 FDASTTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNIN 420
           FDA+ TVVVEG+EFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQ+DVNIN
Sbjct: 361 FDAAITVVVEGVEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQQDVNIN 420

Query: 421 VPTAPEVGLYLDECLFTSYNQKWKDTHEEVSMKAYEEVAEDFKMKQIYSHIASTEHKDGA 480
           VPTAPEVGLYLDECLFTSYNQKWKD+HEEVSMKAYEEVAEDFKMK IYSHI STEHK+GA
Sbjct: 421 VPTAPEVGLYLDECLFTSYNQKWKDSHEEVSMKAYEEVAEDFKMKHIYSHIGSTEHKEGA 480

Query: 481 VALWLHSLNHRNYPDLRVIDEVAKIAKNTSVDMETQAET 520
           VALWLHSLNHRNYPDLRV DE A  AKNTSVD+ETQAET
Sbjct: 481 VALWLHSLNHRNYPDLRVADEAADNAKNTSVDIETQAET 519

[Close]

BLAST of IVF0020427 vs. NCBI nr
Match: XP_008452911.1 (PREDICTED: tRNA pseudouridine synthase A, mitochondrial-like [Cucumis melo] >XP_008452912.1 PREDICTED: tRNA pseudouridine synthase A, mitochondrial-like [Cucumis melo] >ADN34131.1 tRNA pseudouridine synthase family protein [Cucumis melo subsp. melo] >KAA0064620.1 tRNA pseudouridine synthase A [Cucumis melo var. makuwa] >TYK19972.1 tRNA pseudouridine synthase A [Cucumis melo var. makuwa])

HSP 1 Score: 1011 bits (2613), Expect = 0.0
Identity = 519/519 (100.00%), Postives = 519/519 (100.00%), Query Frame = 0


Query: 1   MEEEPPTETRRSPPQPLIEEPDPKKLKMSSTTTTSDDEDVNSNSAAPKKQRYKRRKVAIF 60
           MEEEPPTETRRSPPQPLIEEPDPKKLKMSSTTTTSDDEDVNSNSAAPKKQRYKRRKVAIF
Sbjct: 1   MEEEPPTETRRSPPQPLIEEPDPKKLKMSSTTTTSDDEDVNSNSAAPKKQRYKRRKVAIF 60

Query: 61  FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV 120
           FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV
Sbjct: 61  FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV 120

Query: 121 GQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD 180
           GQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD
Sbjct: 121 GQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD 180

Query: 181 PNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKTAITESDISSNIVNA 240
           PNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKTAITESDISSNIVNA
Sbjct: 181 PNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKTAITESDISSNIVNA 240

Query: 241 IEVSDVQANGVLEDSATTSDSNKQTSVLVENTATELTVASEGIAKSESSEMSGLGNDSEI 300
           IEVSDVQANGVLEDSATTSDSNKQTSVLVENTATELTVASEGIAKSESSEMSGLGNDSEI
Sbjct: 241 IEVSDVQANGVLEDSATTSDSNKQTSVLVENTATELTVASEGIAKSESSEMSGLGNDSEI 300

Query: 301 VVSKESVNGKEKPSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFIVS 360
           VVSKESVNGKEKPSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFIVS
Sbjct: 301 VVSKESVNGKEKPSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFIVS 360

Query: 361 FDASTTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNIN 420
           FDASTTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNIN
Sbjct: 361 FDASTTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNIN 420

Query: 421 VPTAPEVGLYLDECLFTSYNQKWKDTHEEVSMKAYEEVAEDFKMKQIYSHIASTEHKDGA 480
           VPTAPEVGLYLDECLFTSYNQKWKDTHEEVSMKAYEEVAEDFKMKQIYSHIASTEHKDGA
Sbjct: 421 VPTAPEVGLYLDECLFTSYNQKWKDTHEEVSMKAYEEVAEDFKMKQIYSHIASTEHKDGA 480

Query: 481 VALWLHSLNHRNYPDLRVIDEVAKIAKNTSVDMETQAET 519
           VALWLHSLNHRNYPDLRVIDEVAKIAKNTSVDMETQAET
Sbjct: 481 VALWLHSLNHRNYPDLRVIDEVAKIAKNTSVDMETQAET 519

[Close]

BLAST of IVF0020427 vs. NCBI nr
Match: XP_004145528.1 (tRNA pseudouridine synthase A [Cucumis sativus] >KGN55475.1 hypothetical protein Csa_012098 [Cucumis sativus])

HSP 1 Score: 986 bits (2548), Expect = 0.0
Identity = 506/519 (97.50%), Postives = 509/519 (98.07%), Query Frame = 0


Query: 1   MEEEPPTETRRSPPQPLIEEPDPKKLKMSSTTTTSDDEDVNSNSAAPKKQRYKRRKVAIF 60
           MEEEPPTETRRSPPQPLIEEP PKKLKMSSTTTTSDDEDVNSNSAAPKKQRYKRRKVAIF
Sbjct: 1   MEEEPPTETRRSPPQPLIEEPQPKKLKMSSTTTTSDDEDVNSNSAAPKKQRYKRRKVAIF 60

Query: 61  FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV 120
           FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV
Sbjct: 61  FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV 120

Query: 121 GQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD 180
           GQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD
Sbjct: 121 GQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD 180

Query: 181 PNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKTAITESDISSNIVNA 240
           PNCHRDRESVKASLGSDNELVKCLECSERGRKVEGL+GKRNFEVKTAITESDISSN VNA
Sbjct: 181 PNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLIGKRNFEVKTAITESDISSNTVNA 240

Query: 241 IEVSDVQANGVLEDSATTSDSNKQTSVLVENTATELTVASEGIAKSESSEMSGLGNDSEI 300
           IEVSDVQANGVLEDSATTSDSNKQTSVLVENTATEL V SEG AKSESSEMSGLG+DSEI
Sbjct: 241 IEVSDVQANGVLEDSATTSDSNKQTSVLVENTATELNVGSEGTAKSESSEMSGLGSDSEI 300

Query: 301 VVSKESVNGKEKPSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFIVS 360
           VVSKESVNGKEK SGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFIVS
Sbjct: 301 VVSKESVNGKEKSSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFIVS 360

Query: 361 FDASTTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNIN 420
           FDAS  VVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNIN
Sbjct: 361 FDASAIVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNIN 420

Query: 421 VPTAPEVGLYLDECLFTSYNQKWKDTHEEVSMKAYEEVAEDFKMKQIYSHIASTEHKDGA 480
           VPTAPEVGLYLDECLFTSYNQKWKDTHEEVSMKAYEE AEDFKMKQIYSHIASTEHKDGA
Sbjct: 421 VPTAPEVGLYLDECLFTSYNQKWKDTHEEVSMKAYEEAAEDFKMKQIYSHIASTEHKDGA 480

Query: 481 VALWLHSLNHRNYPDLRVIDEVAKIAKNTSVDMETQAET 519
           VALWLHSLNHRNYPDLRVIDEVAKIAKN S+DMETQAET
Sbjct: 481 VALWLHSLNHRNYPDLRVIDEVAKIAKNASLDMETQAET 519

[Close]

BLAST of IVF0020427 vs. NCBI nr
Match: XP_038899088.1 (tRNA pseudouridine synthase A [Benincasa hispida])

HSP 1 Score: 952 bits (2461), Expect = 0.0
Identity = 491/519 (94.61%), Postives = 499/519 (96.15%), Query Frame = 0


Query: 1   MEEEPPTETRRSPPQPLIEEPDPKKLKMSSTTTTSDDEDVNSNSAAPKKQRYKRRKVAIF 60
           MEEEPPTETR SPPQPLIEEPDPKKLKMSSTTTTSDDEDVNS+SAAPKKQRYKRRKVAIF
Sbjct: 1   MEEEPPTETRISPPQPLIEEPDPKKLKMSSTTTTSDDEDVNSSSAAPKKQRYKRRKVAIF 60

Query: 61  FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV 120
           FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV
Sbjct: 61  FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV 120

Query: 121 GQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD 180
           GQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD
Sbjct: 121 GQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD 180

Query: 181 PNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKTAITESDISSNIVNA 240
           PNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKTAI ES ISSN  NA
Sbjct: 181 PNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKTAIIESHISSNTGNA 240

Query: 241 IEVSDVQANGVLEDSATTSDSNKQTSVLVENTATELTVASEGIAKSESSEMSGLGNDSEI 300
           IEVSDVQ N VLED+AT SDSNKQ+SVLVEN+ TEL V SE  AKS  SEMSGLGNDSEI
Sbjct: 241 IEVSDVQENRVLEDNATISDSNKQSSVLVENSCTELNVESESTAKSVGSEMSGLGNDSEI 300

Query: 301 VVSKESVNGKEKPSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFIVS 360
           VVSKESVNG EKPSGSFKY EEEKARFNRILK+YVGTHNFHNFTTRTKAEDPAARRFIVS
Sbjct: 301 VVSKESVNGNEKPSGSFKYCEEEKARFNRILKNYVGTHNFHNFTTRTKAEDPAARRFIVS 360

Query: 361 FDASTTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNIN 420
           FDASTTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNIN
Sbjct: 361 FDASTTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNIN 420

Query: 421 VPTAPEVGLYLDECLFTSYNQKWKDTHEEVSMKAYEEVAEDFKMKQIYSHIASTEHKDGA 480
           VPTAPEVGLYLDECLFTSYNQKWKD+HEEVSMKAYEEVAEDFKMKQIYSHIASTEHKDGA
Sbjct: 421 VPTAPEVGLYLDECLFTSYNQKWKDSHEEVSMKAYEEVAEDFKMKQIYSHIASTEHKDGA 480

Query: 481 VALWLHSLNHRNYPDLRVIDEVAKIAKNTSVDMETQAET 519
           VALWLHSLNHRNYPDLR +DEVA+IA NTSV METQAET
Sbjct: 481 VALWLHSLNHRNYPDLRAVDEVAEIAMNTSVAMETQAET 519

[Close]

BLAST of IVF0020427 vs. NCBI nr
Match: XP_022975443.1 (tRNA pseudouridine synthase A [Cucurbita maxima])

HSP 1 Score: 941 bits (2431), Expect = 0.0
Identity = 480/519 (92.49%), Postives = 495/519 (95.38%), Query Frame = 0


Query: 1   MEEEPPTETRRSPPQPLIEEPDPKKLKMSSTTTTSDDEDVNSNSAAPKKQRYKRRKVAIF 60
           MEEEPPT+TRRSPPQP IEEP+PKKLKMSSTTTTSDDEDVNSNSAAPKKQRYKRRK+AIF
Sbjct: 1   MEEEPPTQTRRSPPQPSIEEPEPKKLKMSSTTTTSDDEDVNSNSAAPKKQRYKRRKIAIF 60

Query: 61  FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV 120
           FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV
Sbjct: 61  FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV 120

Query: 121 GQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD 180
           GQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD
Sbjct: 121 GQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD 180

Query: 181 PNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKTAITESDISSNIVNA 240
           PNCHRDRESVKASLGS NELVKCLECSERGRKVEGLMGKRNFEVKTAI+ESDIS N  NA
Sbjct: 181 PNCHRDRESVKASLGSGNELVKCLECSERGRKVEGLMGKRNFEVKTAISESDISPNFGNA 240

Query: 241 IEVSDVQANGVLEDSATTSDSNKQTSVLVENTATELTVASEGIAKSESSEMSGLGNDSEI 300
           IEVSDVQANG LED+AT SDS K+TSVLVEN+ TEL V SE IAKSE SEMSGLGNDSE 
Sbjct: 241 IEVSDVQANGALEDNATASDSIKETSVLVENSGTELNVGSEAIAKSEGSEMSGLGNDSEN 300

Query: 301 VVSKESVNGKEKPSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFIVS 360
           VVSK+SVN  EKPSGSFKYGEEEKARFNRILK YVGTHNFHNFTTRTKAEDPAARRFI+S
Sbjct: 301 VVSKDSVNRNEKPSGSFKYGEEEKARFNRILKKYVGTHNFHNFTTRTKAEDPAARRFIIS 360

Query: 361 FDASTTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNIN 420
           FDA+ TVVVEG+EFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQ+DVNIN
Sbjct: 361 FDAAITVVVEGVEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQQDVNIN 420

Query: 421 VPTAPEVGLYLDECLFTSYNQKWKDTHEEVSMKAYEEVAEDFKMKQIYSHIASTEHKDGA 480
           VPTAPEVGLYLDECLFTSYNQKWKD+HEEVSMKAYEEVAEDFKMK IYSHI STEHK+GA
Sbjct: 421 VPTAPEVGLYLDECLFTSYNQKWKDSHEEVSMKAYEEVAEDFKMKHIYSHIGSTEHKEGA 480

Query: 481 VALWLHSLNHRNYPDLRVIDEVAKIAKNTSVDMETQAET 519
           VALWLHSLNHRNYPDLRV DE A  AKNTSVD+ETQAET
Sbjct: 481 VALWLHSLNHRNYPDLRVADEAADNAKNTSVDIETQAET 519

[Close]

BLAST of IVF0020427 vs. NCBI nr
Match: KAG6591243.1 (hypothetical protein SDJN03_13589, partial [Cucurbita argyrosperma subsp. sororia] >KAG7024127.1 PUS1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 926 bits (2392), Expect = 0.0
Identity = 476/520 (91.54%), Postives = 491/520 (94.42%), Query Frame = 0


Query: 1   MEEEPPTETRRSPPQPLIEEPDPKKLKMSSTTTTSDDEDVNSNSAAPKKQRYKRRKVAIF 60
           MEEEPPT+TRRSPPQP IEEP+PKKLKMSSTTTTSDDEDVNSNSAAPKKQRYKRRK+AIF
Sbjct: 1   MEEEPPTQTRRSPPQPSIEEPEPKKLKMSSTTTTSDDEDVNSNSAAPKKQRYKRRKIAIF 60

Query: 61  FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV 120
           FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV
Sbjct: 61  FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV 120

Query: 121 GQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD 180
           GQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD
Sbjct: 121 GQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD 180

Query: 181 PNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKTAITESDISSNIVNA 240
           PNCHRDRESVKASLGS NELVKCLECSERGRKVEGLMGKRNFEVKTAI+ESDIS N  NA
Sbjct: 181 PNCHRDRESVKASLGSGNELVKCLECSERGRKVEGLMGKRNFEVKTAISESDISPNSGNA 240

Query: 241 IEVSDVQANGVLEDSATTSDSNKQTSVLVENTATELTVASEGIAKSESSEMSGLGNDSEI 300
           IEVSDVQANG LED+AT SDS K+TSVLVEN+ TEL V  E  AK E SEMSGLGNDSE 
Sbjct: 241 IEVSDVQANGALEDNATASDSIKETSVLVENSGTELNVGGEATAKIEGSEMSGLGNDSEN 300

Query: 301 VVSKESVNGKEKPSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFIVS 360
           V SKESVN  EK SGSF+YG+EEKARFNRILK YVGTHNFHNFTTRTKAEDPAARRFI+S
Sbjct: 301 VASKESVNRNEKSSGSFEYGKEEKARFNRILKKYVGTHNFHNFTTRTKAEDPAARRFIIS 360

Query: 361 FDASTTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNIN 420
           FDA+TTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQ+DVNIN
Sbjct: 361 FDAATTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQQDVNIN 420

Query: 421 VPTAPEVGLYLDECLFTSYNQKWKDTHEEVSMKAYEEVAEDFKMKQIYSHIASTEHKDGA 480
           VPTAPEVGLYLDECLFTSYNQKWKD+HEEVSMKAYEEVAEDFKMK IYSHI STEHK+GA
Sbjct: 421 VPTAPEVGLYLDECLFTSYNQKWKDSHEEVSMKAYEEVAEDFKMKHIYSHIGSTEHKEGA 480

Query: 481 VALWLHSLNHRNYPDLRVIDEVAKI-AKNTSVDMETQAET 519
           VALWLHSLNHRNYPDLRV DE A   AKNTSVD+ETQAET
Sbjct: 481 VALWLHSLNHRNYPDLRVADEAADDNAKNTSVDIETQAET 520

[Close]

BLAST of IVF0020427 vs. TAIR 10
Match: AT1G20370.1 (Pseudouridine synthase family protein )

HSP 1 Score: 546.6 bits (1407), Expect = 2.2e-155
Identity = 300/504 (59.52%), Postives = 369/504 (73.21%), Query Frame = 0


Query: 1   MEEEPPTETRRSPPQPLIEEPDPKKLKMSSTTTTSDDEDV---NSNSAAPKKQRYKRRKV 60
           ME + P   +         EPD KKLK+S+T   +D   V    SN A  +  +Y+RRKV
Sbjct: 1   MENQEPDIEKLPRDDVPDREPDHKKLKISTTGQETDSSAVTTGGSNVAERRAPKYRRRKV 60

Query: 61  AIFFAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGV 120
           AI FA+CGVGYQGMQKNPGAKTIE +LEEAL+ AGAVP+ DR   + Y+WARSARTDKGV
Sbjct: 61  AIVFAFCGVGYQGMQKNPGAKTIEGELEEALFHAGAVPDADRNKPRNYEWARSARTDKGV 120

Query: 121 SAVGQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVF 180
           SAVGQVVSGRFY+DPPGFV+RLNS L  QIR+FGYKRV  SF++KKFCDRRRYVYLIPVF
Sbjct: 121 SAVGQVVSGRFYVDPPGFVERLNSKLPDQIRVFGYKRVAPSFSSKKFCDRRRYVYLIPVF 180

Query: 181 ALDPNCHRDRESVKASLGSDNELVKCLECSERGRKVE-GLMGKRN--FEVKTAITESDIS 240
           ALDP  H + E V+  LG   E VKC+ECSE+G K+  G+MGK     + K+   +SDIS
Sbjct: 181 ALDPCVHGEAEMVRTDLG--YEYVKCVECSEKGYKIPLGVMGKDTDCCDTKSLEIQSDIS 240

Query: 241 SNIVNAIEVSDVQANGVLEDSATTSDSNKQTSVLVENTATELTVASEGIAKSESSEMSGL 300
           S+  +AI  +DV+    L  S  ++++N  + VL     + L+  +EG+ +S +     +
Sbjct: 241 SSTCDAIG-ADVKCE-TLSSSVPSAENNLNSEVLDGADVSALSSKAEGMEESNTLAKVEM 300

Query: 301 GN-DSEIVVSKESVNGKEKPSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPA 360
            N + E +   ES          F YGE+EK RFNRIL +YVG+HNFHNFTTRTKA DPA
Sbjct: 301 NNGEGEDMTKSES---------KFCYGEKEKERFNRILSYYVGSHNFHNFTTRTKAADPA 360

Query: 361 ARRFIVSFDASTTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKAL 420
           A R+I+SF+A+T + ++G +FVKCEVVGQSFMLHQIRKM+GLAVAIMRN APESLIE + 
Sbjct: 361 ANRYILSFNANTVINLDGKDFVKCEVVGQSFMLHQIRKMIGLAVAIMRNYAPESLIEASF 420

Query: 421 QKDVNINVPTAPEVGLYLDECLFTSYNQKWKDTHEEVSMKAYEEVAEDFKMKQIYSHIAS 480
           +KDV INVP APEVGLYLDEC FTSYN+++K +HEEVSM+ Y+EVAE+FK K +YSHI S
Sbjct: 421 KKDVRINVPMAPEVGLYLDECFFTSYNKRFKGSHEEVSMEEYKEVAEEFKWKYVYSHIGS 480

Query: 481 TEHKDGAVALWLHSLNHRNYPDLR 498
            E KDGAVA+WLHSLN RNYPDLR
Sbjct: 481 AEEKDGAVAIWLHSLNQRNYPDLR 491

[Close]

BLAST of IVF0020427 vs. TAIR 10
Match: AT1G76120.1 (Pseudouridine synthase family protein )

HSP 1 Score: 525.8 bits (1353), Expect = 4.0e-149
Identity = 284/470 (60.43%), Postives = 342/470 (72.77%), Query Frame = 0


Query: 31  TTTTSDDEDVNSNSAAPKKQRYKRRKVAIFFAYCGVGYQGMQKNPGAKTIEADLEEALYL 90
           TTTT ++ D++ +    + Q     KVAI FA+CGVGYQGMQKNPGAKTIE +LEEAL+ 
Sbjct: 3   TTTTDEEIDLSCDGGVTEPQ-----KVAIIFAFCGVGYQGMQKNPGAKTIEGELEEALFH 62

Query: 91  AGAVPEQDRGLSKRYDWARSARTDKGVSAVGQVVSGRFYIDPPGFVDRLNSNLSPQIRIF 150
           AGAVPE  RG  K YD+ARSARTDKGVSAVGQVVSGRF +DP GFV+RLNSNL  QIRIF
Sbjct: 63  AGAVPESIRGKPKLYDFARSARTDKGVSAVGQVVSGRFIVDPLGFVNRLNSNLPNQIRIF 122

Query: 151 GYKRVTASFNAKKFCDRRRYVYLIPVFALDPNCHRDRESVKASLGSDNELVKCLECSERG 210
           GYK VT SF++KKFCDRRRYVYL+PVFALDP  HRDRE+V ASLG   E VKC ECSERG
Sbjct: 123 GYKHVTPSFSSKKFCDRRRYVYLLPVFALDPISHRDRETVMASLGPGEEYVKCFECSERG 182

Query: 211 RKV-EGLMGK---RNFEVKTAITESDISSNIVNAIEVSDVQANGVLEDSATTSDSNKQTS 270
           RK+  GL+GK    NF  K+   +SDISSN  +A+  SD++   +  + A     + +  
Sbjct: 183 RKIPPGLVGKWKGTNFGTKSLDFQSDISSNNSSALR-SDIKIEALSSNLAGLCSVDVEVG 242

Query: 271 VLVENTATELTVASEGIAKSESSEMSGLGNDSEIVVSKESVNGKEKPSGSFKYGEEEKAR 330
            + E++    T +SE   KS+                             F YGE+EK R
Sbjct: 243 RIQEDSCKLNTNSSETKVKSK-----------------------------FCYGEKEKER 302

Query: 331 FNRILKHYVGTHNFHNFTTRTKAEDPAARRFIVSFDASTTVVVEGIEFVKCEVVGQSFML 390
           F+RIL  YVG++NFHNFTTRTKA+DP A R I+SF A+T + ++GI+F+KCEV+G+SFML
Sbjct: 303 FSRILSCYVGSYNFHNFTTRTKADDPTANRQIISFTANTVINLDGIDFIKCEVLGKSFML 362

Query: 391 HQIRKMMGLAVAIMRNCAPESLIEKALQKDVNINVPTAPEVGLYLDECLFTSYNQKWKDT 450
           HQIRKMMGLAVAIMRNCA ESLI+ A  KDVNI VP APEVGLYLDEC FTSYN+ ++D+
Sbjct: 363 HQIRKMMGLAVAIMRNCASESLIQSAFSKDVNITVPMAPEVGLYLDECFFTSYNRNFEDS 422

Query: 451 HEEVSMKAYEEVAEDFKMKQIYSHIASTEHKDGAVALWLHSLNHRNYPDL 497
           HEEVSM+AY+E AE FK+K IYSHI +TE K G +ALWLHSLN+RNYPDL
Sbjct: 423 HEEVSMEAYKEEAEAFKLKHIYSHIGATERKYGNMALWLHSLNYRNYPDL 437

[Close]

BLAST of IVF0020427 vs. TAIR 10
Match: AT1G76120.2 (Pseudouridine synthase family protein )

HSP 1 Score: 399.4 bits (1025), Expect = 4.3e-111
Identity = 224/389 (57.58%), Postives = 272/389 (69.92%), Query Frame = 0


Query: 31  TTTTSDDEDVNSNSAAPKKQRYKRRKVAIFFAYCGVGYQGMQKNPGAKTIEADLEEALYL 90
           TTTT ++ D++ +    + Q     KVAI FA+CGVGYQGMQKNPGAKTIE +LEEAL+ 
Sbjct: 3   TTTTDEEIDLSCDGGVTEPQ-----KVAIIFAFCGVGYQGMQKNPGAKTIEGELEEALFH 62

Query: 91  AGAVPEQDRGLSKRYDWARSARTDKGVSAVGQVVSGRFYIDPPGFVDRLNSNLSPQIRIF 150
           AGAVPE  RG  K YD+ARSARTDKGVSAVGQVVSGRF +DP GFV+RLNSNL  QIRIF
Sbjct: 63  AGAVPESIRGKPKLYDFARSARTDKGVSAVGQVVSGRFIVDPLGFVNRLNSNLPNQIRIF 122

Query: 151 GYKRVTASFNAKKFCDRRRYVYLIPVFALDPNCHRDRESVKASLGSDNELVKCLECSERG 210
           GYK VT SF++KKFCDRRRYVYL+PVFALDP  HRDRE+V ASLG   E VKC ECSERG
Sbjct: 123 GYKHVTPSFSSKKFCDRRRYVYLLPVFALDPISHRDRETVMASLGPGEEYVKCFECSERG 182

Query: 211 RKV-EGLMGK---RNFEVKTAITESDISSNIVNAIEVSDVQANGVLEDSATTSDSNKQTS 270
           RK+  GL+GK    NF  K+   +SDISSN  +A+  SD++   +  + A     + +  
Sbjct: 183 RKIPPGLVGKWKGTNFGTKSLDFQSDISSNNSSALR-SDIKIEALSSNLAGLCSVDVEVG 242

Query: 271 VLVENTATELTVASEGIAKSESSEMSGLGNDSEIVVSKESVNGKEKPSGSFKYGEEEKAR 330
            + E++    T +SE   KS+                             F YGE+EK R
Sbjct: 243 RIQEDSCKLNTNSSETKVKSK-----------------------------FCYGEKEKER 302

Query: 331 FNRILKHYVGTHNFHNFTTRTKAEDPAARRFIVSFDASTTVVVEGIEFVKCEVVGQSFML 390
           F+RIL  YVG++NFHNFTTRTKA+DP A R I+SF A+T + ++GI+F+KCEV+G+SFML
Sbjct: 303 FSRILSCYVGSYNFHNFTTRTKADDPTANRQIISFTANTVINLDGIDFIKCEVLGKSFML 356

Query: 391 HQIRKMMGLAVAIMRNCAPESLIEKALQK 416
           HQIRKMMGLAVAIMRNCA ESLI+ A  K
Sbjct: 363 HQIRKMMGLAVAIMRNCASESLIQSAFSK 356

[Close]

BLAST of IVF0020427 vs. TAIR 10
Match: AT2G30320.1 (Pseudouridine synthase family protein )

HSP 1 Score: 115.2 bits (287), Expect = 1.6e-25
Identity = 117/427 (27.40%), Postives = 180/427 (42.15%), Query Frame = 0


Query: 23  PKKLKMSST-----TTTSDDEDVNSNSAAPKKQRYKRRKVAIFFAYCGVGYQGMQ---KN 82
           P  L+ SS+     ++TS     + N  A K + Y+++KV I   Y G  Y+G+Q    +
Sbjct: 26  PALLRFSSSAAFQPSSTSLSPPSSDNFLADKWESYRKKKVVIRIGYVGTDYRGLQIQRDD 85

Query: 83  PGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAVGQVVSGRFYI---- 142
           P  KTIE +LE A+Y AG + + + G   +  WARS+RTDKGV ++   +S +  I    
Sbjct: 86  PSIKTIEGELEVAIYKAGGIRDSNYGDLHKIGWARSSRTDKGVHSLATSISLKMEIPETA 145

Query: 143 ---DPPGFV--DRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALDPNCHR 202
              DP G V    ++ +L   IR+F        F+ ++ C  R+Y YL+PV  L      
Sbjct: 146 WKDDPQGTVLAKCISKHLPENIRVFSVLPSNRRFDPRRECTLRKYSYLLPVDVL------ 205

Query: 203 DRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKTAITESDISSNIVNAIEVSD 262
               +K S  SD       + +E  ++ EG     N+  ++               E   
Sbjct: 206 ---GIKNSFTSDEIDYHITDFNEILKEFEGEYPFHNYTQRSRYRRKS---------EQKI 265

Query: 263 VQANGVLEDSATTSDSNKQTSVLVENTATELTVASEGIAKSESSEMSGLGNDSEIVVSKE 322
            Q NG             +    ++ + +E    +      E  E    G   E VV+ +
Sbjct: 266 KQRNG----------RPPREPKSIKASESEFREENHIEIGEEEEEKEVDGESDEHVVTPD 325

Query: 323 SVNGKEKPSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRT-KAEDPAARRFIVSFDAS 382
           S N +      + Y  +E  + +       G H    F  R+ K E+             
Sbjct: 326 SDNSQVYSRAKWLYEPDETDKIS-------GAHFRKVFRCRSGKLENSL----------- 385

Query: 383 TTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNINVPTA 432
                 G  FV+  + G+SFMLHQIRKM+G AVA+ R   P  +I  +L K   I +P A
Sbjct: 386 ------GFGFVEISIWGESFMLHQIRKMIGTAVAVKRELLPRDIIRLSLNKFTRIVLPLA 400

[Close]

BLAST of IVF0020427 vs. TAIR 10
Match: AT1G20290.1 (SWI-SNF-related chromatin binding protein )

HSP 1 Score: 68.2 bits (165), Expect = 2.3e-11
Identity = 36/62 (58.06%), Postives = 41/62 (66.13%), Query Frame = 0


Query: 437 TSYNQKWKDTHEEVSMKAYEEVAED-FKMKQIYSHIASTEHKDGAVALWLHSLNHRNYPD 496
           TS    W+    EVS+   +E   D FK K +YSHI S E KDGAVA+WLHSLN RNYPD
Sbjct: 77  TSARISWR----EVSVDDDQEQEHDQFKWKHVYSHIGSAEEKDGAVAIWLHSLNQRNYPD 134

Query: 497 LR 498
           LR
Sbjct: 137 LR 134

The following BLAST results are available for this feature:

BLAST of IVF0020427 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q59S63	1.4e-53	33.26	tRNA pseudouridine synthase 1 OS=Candida albicans (strain SC5314 / ATCC MYA-2876...	[more]
Q6CC39	1.2e-49	32.02	tRNA pseudouridine synthase 1 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) O...	[more]
Q9Y606	7.8e-49	30.50	tRNA pseudouridine synthase A OS=Homo sapiens OX=9606 GN=PUS1 PE=1 SV=3	[more]
Q9WU56	1.7e-48	30.30	tRNA pseudouridine synthase A OS=Mus musculus OX=10090 GN=Pus1 PE=1 SV=2	[more]
Q4KM92	3.9e-48	30.30	tRNA pseudouridine synthase A OS=Rattus norvegicus OX=10116 GN=Pus1 PE=1 SV=2	[more]

BLAST of IVF0020427 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3BV07	7.3e-292	100.00	tRNA pseudouridine synthase A, mitochondrial-like OS=Cucumis melo OX=3656 GN=LOC...	[more]
A0A5A7VBT0	7.3e-292	100.00	tRNA pseudouridine synthase A OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc...	[more]
E5GCD2	7.3e-292	100.00	tRNA pseudouridine synthase family protein OS=Cucumis melo subsp. melo OX=412675...	[more]
A0A0A0L0J9	2.5e-284	97.50	PseudoU_synth_1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G65...	[more]
A0A6J1IJ76	9.3e-271	92.49	tRNA pseudouridine synthase A OS=Cucurbita maxima OX=3661 GN=LOC111474781 PE=3 S...	[more]

BLAST of IVF0020427 vs. NCBI nr


Match Name	Identity	Description
XP_008452911.1	100.00	PREDICTED: tRNA pseudouridine synthase A, mitochondrial-like [Cucumis melo] >XP_...	[more]
XP_004145528.1	97.50	tRNA pseudouridine synthase A [Cucumis sativus] >KGN55475.1 hypothetical protein...	[more]
XP_038899088.1	94.61	tRNA pseudouridine synthase A [Benincasa hispida]	[more]
XP_022975443.1	92.49	tRNA pseudouridine synthase A [Cucurbita maxima]	[more]
KAG6591243.1	91.54	hypothetical protein SDJN03_13589, partial [Cucurbita argyrosperma subsp. sorori...	[more]

BLAST of IVF0020427 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G20370.1	2.2e-155	59.52	Pseudouridine synthase family protein	[more]
AT1G76120.1	4.0e-149	60.43	Pseudouridine synthase family protein	[more]
AT1G76120.2	4.3e-111	57.58	Pseudouridine synthase family protein	[more]
AT2G30320.1	1.6e-25	27.40	Pseudouridine synthase family protein	[more]
AT1G20290.1	2.3e-11	58.06	SWI-SNF-related chromatin binding protein	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR020095	Pseudouridine synthase I, TruA, C-terminal	GENE3D	3.30.70.660		coord: 298..499 e-value: 2.5E-55 score: 189.2
IPR020094	Pseudouridine synthase TruA/RsuA/RluB/E/F, N-terminal	GENE3D	3.30.70.580		coord: 45..165 e-value: 1.2E-28 score: 101.6
IPR020097	Pseudouridine synthase I, TruA, alpha/beta domain	PFAM	PF01416	PseudoU_synth_1	coord: 331..436 e-value: 2.0E-14 score: 54.0
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..49
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..26
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 27..47
None	No IPR available	PANTHER	PTHR11142:SF24	TRNA PSEUDOURIDINE SYNTHASE	coord: 31..497
IPR001406	Pseudouridine synthase I, TruA	PANTHER	PTHR11142	PSEUDOURIDYLATE SYNTHASE	coord: 31..497
IPR041708	Pseudouridine synthase PUS1/ PUS2-like	CDD	cd02568	PseudoU_synth_PUS1_PUS2	coord: 59..436 e-value: 2.27194E-106 score: 316.092
IPR020103	Pseudouridine synthase, catalytic domain superfamily	SUPERFAMILY	55120	Pseudouridine synthase	coord: 53..446

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
IVF0020427.1	IVF0020427.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:1990481	mRNA pseudouridine synthesis
biological_process	GO:0031119	tRNA pseudouridine synthesis
biological_process	GO:0001522	pseudouridine synthesis
biological_process	GO:0009451	RNA modification
cellular_component	GO:0005634	nucleus
molecular_function	GO:0009982	pseudouridine synthase activity
molecular_function	GO:0003723	RNA binding