IVF0019533 (gene) Melon (IVF77) v1

Overview
NameIVF0019533
Typegene
OrganismCucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
DescriptionE3 ubiquitin-protein ligase UPL4
Locationchr04: 30891351 .. 30900393 (-)
RNA-Seq ExpressionIVF0019533
SyntenyIVF0019533
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTTCATAGATATCTTTAAGCTCTGGCGTTCTCTCGCTCTGTTTTTTCATCATCGTCGACGTCAAAAGAGCTTTACAATTTCGATCACCGATAAAAGCATCTGCCGTCAGAGTTGCGTTCCGCCGTCGACGGAGAAGTTTTCTCCCTCACAAACTCCCGTATTTTTTCATCGTGCTTTTGGAATTTCGTGAGTCTTCGGTTTGCAGCGCTAAGGTACTAAGAAATATAACATAGTTTTCTCTTGTCTGATCGTAAATCCGTCTTTACATTCGTGATTTTTGATCTCGTTCTTTATCGCATTCGCTTTGGCTTTCGTATTTTTTGAATGATAAAAGAGGACTAGTTACGACTTACGAGCTTACTAGAGAAACTTAGTTATGTGCTCAAGATTGTCTAGCATATATGATAGATATTTTATTTGTCAATGGTGGTTTAATCCGTGGAGTTTTCGGGTCCTGTGTTAGTCTAGGAGCAGTAGAGGTTCGTTTATTTTTGTTCAAATTGACGGTGAACATAGTTTATTTTATATTTAGCTCTTATGGTTTGAGACAGACAATTCTCAGTGGAATGTTCCAATTCCTATCAGATTATCCTGTGAGTTGCGGATTGGAATGTTCACCTTTGAGTGCTTTTCAATCTATTGAAGTGGAGTTGTTAGTGTTGTGCTAAAATACTGTTTGTTATCGCGTCTTTTATTTAATGTTTTTCTTTCTTTGTAGTTTGGTTCTGATCATTGATAATATGTAAATTATTGAAGCACAAAAGTTGGTCAATCTTATGCAATGTTGTATCTCTTGATTATATAGGAGGGTGTTTACTCTATTAGAGCTAATAAATGATAAATGGGAAATCGTGGCCAAAAGCGTACTGAAATGGTGGATAGATTACCGGCTGATAAACGAGCATGCAGTTCATTGGAATTCAGACCAAGCTCATCAAATTCATCTATGCAAATGCACGTGACTTCGACCAATTCTAGTCCTGGCATCCATGATAATGACATGGACACTTCATCGTCTGCTTCTGCTTCTAGTCGTTCAGAGGGAGAACATGATAAGGATTCAGCCTACGGGTCTTGTGATTCTGATGATGCTGAACAGAAACATAGTGATCTTCGGAATTATCAGAGACAAAGATCATCAGGTGATCATGGACGATTTAAAAGGCTTTTGACAAGCTTGGGTGAAGAATCTGAGTCATCTGTACAAACAGAACTGCTGAGGGAACTATGTGAGGTTTTATCATTCTGCACGGAGAATTCACTATCGAGTATGACATCAGATTCGTTATCTATAATTCTTGTAAATCTGGTGAAACTTGATAGCGACTCAGATATAGTGCTATTGGCTTTAAGGGCTTTAACTTATTTATGTGATGCTTACCCCAGAGCATCTAGTTTTATAGTTCGTCATGGTGGAGTTCCTGCCTTCTGTAAGAGGCTGGGAGCTATTGAGTACTCGGATGTTGCTGAACAGGTGATACGGTCATGCACCCTTAATTGTTCTTTTTAGTTCATTTGTCAAATTATCTTTGAATGCTGAATAGTTTTGCTATATTTTGAAGGACTTTTTGTTTAAATGTAGTGTTTCCAAGCATTGGAAAAATATCACAGGAGCACCCTGTTGCATGCTTAGAGGGTGGTGCAGTTATGGCTGTTTTAACTTTTATTGATTTTTTTCCTACGATCATACAAGTAAGCCATTTCCCTTTCTGGATGTTAATTCAATCTCGACAGTATGGTTGAACTCGTGTTCCTATTATTTTGTTCCACGAAATCCCATGATTGTAAAGATCGAAAGTTTAAAGTTAATTTGTTTTGGATCAGAGAACGGCTCTTCGTATAGTGGTGAATGTCTGCAAAAAACTTCCTTCCGAGTGTCCACCAAATTTGATTGAGGCTGTTCCTATACTATGCAATCTCCTTCAATATGATGATGAGGAGGTTTGAATAAACTTACTTGAGTGCTTCATATTGCTTTATTGTATTTTATTTATAAATCTCTTCTTGTTATAATGTCATATTTCAGCTGGTAGAGAATGTGGCTCGATGCATGATTAAAATAGCTGAGTGCGTCCATCAATCCTCCGAGCTTTTGGATGGGCTTTGTCAGCATGGGCTGATTCAACATGCCATTCGCCTTATAAACTTGAATAGCCGGACAACTTTGTCCCAGACCATTTACAATGTAAGTAAAGAATCATACAAGTAAGGGACCAATGGTTTAATATTTTGTGTATTTTTATTTTAGTACAAGGCAGAGGAACGTTTCCCTGTAGCATCTACTACCATTTTTAGGGAAGAATGGTGAAATGACCATCAACTTGTTTTGCAGGATTTGCTTGGTGTACTTATCAAACTTGCTTCAGGATCCATTGTTGCTTTTGAAACCCTTTATGAGCTAAACATTAGCAACACTTTGAAGGACATATTGTCTGCTTACAATCTCTCCCATGGGGTGTCTTCTTCGTGTGCTGTAGTTGATGGGCAGCGGAACCAGGTATTAGACTATGAACATGTACTTTTTTGAAACCATGGAAAGACCGACTGTGCAGTGTTAATCTTTTGTAAAAAATAATTCTAGGAAACTTACAAGTGAGACTTCCCCCTTATTACTTATTCTGAAAAACACTTCACAGAAATCTTTTCTTCCAAATTGCTTACTAGAACTGTCTTTTTAAAGTTTCTAGTCATCTTTTTTAATCATGCATCGGCAATATTTCATGTAAACCTCCTCTTGGGGTTTAGGAGTGTTTTATGCTTCTTTCTCATTTCATTCATCAATTACATTTTCTAAGTTCTCTGCACAAATAAAAGTGATAGTTCAATAGCTCATAATTGGCTTACATCGGGTCTAGAATTCAAATTTCATTCTGCCTTTGTCTTCCCCTCCAGGTTTGTAGGGTCTGTTTGTGTGTGTGTGTGTGTTTTAAATAAAAATTTTATGGGGATTTACTGTGGTTGGCTTTTGCCCAGGTATGTGAAGTCCTGAAATTGCTCAACGAGCTTCTCCCAACTGAAGATGCGAAGACAGAACAATTGTCTGAAAAAGTGTCTTTCTTAGTCGGTAATCCCAAACAACTGCAGAAATTTGGCTTGGATATACTTCCTTTGCTTGTTCAGGTGGTGATTATTATAAGTATAAGCTAAAAAAAAAAAAGCTCTAGCCCCTACCATCTCCTATAACATCATATTTTCGCTGCTGCAGGTGGTTAGTTCTGGTGCAAACTTGTATGTCTGTTGCGGCTGCTTAACTATTATCTACAAGTTTGTCTGTTTGGGTGAATCCGACATGCTTGTTGAGTTACTTGAGAATTCCAACATTTCTAGGTGAATGATGCCTGATATATATATATATATATATATATATATATATTTGTGTGTGTGTGTATAATGGTATTTTCTAATTGCTTTCAGAAAGTCTAATGATCTATTCTATCTAATTTGTCAGTTTCCTGGCTGGAGTGTTTACTCGAAAAGACCACCACGTGCTGATGTTAGGTTTAAAAATCACTGAGATTATCCTGCAAAAGCTCGCTAGTATATTTTTGAAATCATTTGTCAAGGAGGGTGTCTATTTTGCTATTGATGCACTGATATCCCCGGAGAAGTATAAACAGTTGATTTTCCCAGTGTTTACTGGTGTCCACTCATCATTTGATTCCTGTCAAAAGTCTTCAAGAGAACATGGGAAATGCTTATGTTATGCCTTTTCGAGTAGCTGTTTCCCCTCTGGGTCAGAAACAGGCAGCTGCAAGCTTGACAAGGATTCTGTTTATAGTCTTGCAAATCATATAAGAAACATTTATTTTGCTGAAGATTTATGTGACACCGATGAAGGAGTAACTGATATTCTTCAAAATCTTCGAACTTTTTCTGGGGCCCTAGATGATCTACTAAACTTGTCCTTAATTAAGGACACTCCAGCTCAGGATGAAGAAAAATTGTATGCTTTGTTAGCTGAGATTATGTCAAAGCTTAAGTGTGGCGAGCCCATATCCACCTTTGAATTTATTGAAAGTGGAATAGTCAAGTCATTTATAAATTACATAACTAATGGTCAGTATCTGAGGAAAAAGGAAGAAGCACAAACTATATCTAGACAATTCTCAATTATAGAAAGAAGATTTGAGGCTTTTGCAAGGCTACTCTTATCTAGTTCTGACCACCCATCAGTAAATTTACCAGTTTTGGCATTGATAAGGAAATTACAAATCTCACTTTCGTCTTTAGAAAACTTTCCTGTTATTTCAAGCCAGGGATTCAAACATAGGAACTATTTTGCTACGGTTCCAAATGCTCGGTGCGTACCACATCCTTGTGTGAAAGTTCGGTTTGTGAGGGGAGATGGAGAAACAAAACTATGTGACATTACCGGGGATATCCTGACTGTTGACCCGTTTTCTTCTTTAGCTGCCATTGAAGGGTTTCTATGGCGCAAAGTTAGCACAAAAAAAACTGAGCAGTCCCCTGAAGAGACTCTGAGGGAGCATCAAATCAAACTACTATCAAATATACGTTCTTGCTTGGGTGTTAACTCAGAGTTAGTGGGGTCTGACATCATGTCCACAGATTTGCCCGAAGTTCAGGTATGTTCCCGTTTTCTTTTTGAAATATTGATGATGTTGAAAGAATTATGAGCTTACTCTTAAATTGCACAATACTTGAAGTAAGGTTATTTGCCATGTAGTGTGGAGGTCTGTTATCTTTAATGTTTATTTATTTATTTATTTTAAATTCAGCGAGATATGGCTAACATACCTAAAGTATTTGGTTTGAGTTTAGGTGTTTTAAGAGAGAGAATAATATCTGATAGCAACTTATGTTGTAACTTGATCGTTGTATAAGTATATATATAGAACTGTGTATAAGATGGATCAGTTCTAGAATTTGAAGCCTCATCCATTGAAAATTTCAATCCAGTCCTTGTGGATTCAACTTTTGCCATTTTTCTACTTCCAAGTAAACTAGGAAAAATATTATGGTTTTATGTGAAAGATTCTTGTTCTGTCTTTCAGTACCTGCTTAATCCATGAATGAAGCATCGATCATATTCTGGACTATGGTAATGTGTGCAGTCCTAAAGTATGCACAAATATTTGTAATTATTCAATAGGCAACGTTATTCTTAGTTGGACTCATTAATTTGTATAAGCTTGTATTCCTTCATTCTTATTTGGAGCCCGTTTTTAAAATTATTTCTATGTGCTTGTATTCTTTCATGCTCAATGATAGTAGTTGTTTCTATTAAATAAAAGGAAATTTGCACACTCCTGATATTATGTTAACCTTGTTTTGGTACAGGTACCTGCAGAAGTCGGTGCTGATGAGAAATCACAGTCTTCTCCTTCTTGCAGTAAGAAAGGCACGGCTCCCAGATTGCTTCTTTACCTTGAGGGAAAGCAGCTGGAGCCCACCATGACAATTTACCAGGCAATTCTCCAACAACACATCAAAGAAAATGAAACTATATCTGGCACGAAAGTATGGAGCCATGTATATACAATAATGTATAGAAGCGCTGGTGAAGTGGAGGACAACACTTGTAACCAATTGTTTTGTGCATCCGACAAAGCTCCAACATTGCAGTTTTCTTCATTTTTCTGTGACATTCTAGATTGTGTTCTGCCTTCTGACTTGGCAAAAGGAAGTCCGGCTTATGATGTTTTATTTCTTCTAAGGAGCATAGAGGGCATGAACAGGATGGCATTTCACATTATGTCACATGAAAGAATTCGTGCTTTTGCCGAAGGAAAAATTGATACCTTGGACAATATAAAGCTATCAGTTCCAACGGTGTCACAGAATGAATTTGTGAATAGCAAACTGACTGAAAAACTTGAACAGCAGATGAGGGATTCTTCTGCTGTTTCTGTCGGTGGCATGCCTTTATGGTGTAAGGAGCTTATGGATTCATGCCCTTTCTTATTTAGTTTCGAGGCAAGGCGCAAGTACTTTCGGATAGTAGTATTTGGCATGCCACAATACCAGCTACATGTGAGATCACACAGTGACTTGGGGACCTCAAATGACGGACGGTCAAGTTCTGGTGGCTTGCCTCGTAAGAAGGTATTAGTACACCGCGACCAAATTCTAGATTCTGCTTCCAAAATGATGGATCAGTATGCACATCAGAAAGTTCTCCTTGAGGTGGAATATGATGAAGAAGTTGGCACTGGGCTTGGTCCAACATTAGAATTCTATACTCTTGTCAGTCGTGAATTTCAGAAGTATGGCCTTGGAATGTGGAGAGGGGATCATGATGCATTTATATCTGGAAAGAGGCTGAACATTGAGGGTAGAGAAACTACTGAATCTCCATTTGGGCTTTTTCCCCGTCCATGGCCATCAACTCTGGACACAGATAAGCTACATCTTCCCGAAGTAATGAAAAAGTTCGTACTTCTAGGACAAATTGTGGCCAAGGCCATTCAAGATGGTAGGGTATTGGATATCTACTTCTCCAAAAGCTTCTATAAACTAATACTTGGGCAGGTACCTGCATGAACTTCTTTCAAGTGCTTTCTCTCATCTTTTTCATATTCCATTACTTGATTGTCTTATTTATTCTTGTAGGAGTTGAGTATTTATGATATCCAATCATTTGATCCAGAGCTCGGTACTGTTCTGCTAGAGTTTCAATCTTTGGTCAACAGAAATAAAATATTGGGAACTGTTTACGAAGAGAACTCATCATCCAAACTCGAATTTTCTTATCATAATACCAATATTGAGGATCTTTGCCTTGATTTTACTCTTCCTGGTTATCCTGATTATTTATTGACTTCGTCTCAAGATAACTCAATGGTAATGCCAATTTTCACTGTGATTATGGAATTTTCTTTTTTGTGGTTGGACTAAATAACACAATACATTTTGACCTTTTCCGCAGGTAAATGCAAAAAATTTGGAGGATTATGTCTCTCTTGTTGCAGATGCTACTTTATATTCTGGAATTTCAAGACAAATAGAAGCATTTAAGTCTGGATTTAATCAGGTTAGCTTCGCTTTGCCCATCTTACACAAGATTATACATCTCATTTTTATTTAGTTGTTCTAGTTAGCACTTGCTGAATAAAAATGATTCTTTATTCCCATTATCTTTATGTTGCATAGCTAAAAGTTTATGGAATTCAGTTCATGTTCTGATATAGCTTTTTTATTTAATCAATGTCTTACTTGAAATATTATCAACTACTTCAATTTCTAGACGTGATATGCTTTATAGCACAATAGAAGGAACAGATGAACTGCAATATTAAAATGCAAGAAACTTTTGAATAAAATTTCATGAGCGTTCTTGTCCTTAAAAGAGAGATGCTCACCAACTTGGTTGGTTTAAAGACAAATTTCCAATTACCATAGAAAATACACTTTTCCCTTGAACATTTATCGGTAACTGATAATCGAGTACTTCTTTTCTTATTGTCAAACCTCAGGTTTTCCCCATAGAACATCTTCAAGTATTTACTGCAGAAGAATTAGAGCGTTTAATATGCGGAGAACAAGACATTTGGGCCGTACGTATATCAGCTAATTATCACATTTACTTAAAATTATTAACCATTAGCTTAGTTCTTTTTAATCTTTTGATGCAGTTGAGTGATCTCCTTGACAATATGAAGTTTGACCATGGATATACATCCAGTAGCCCCTCCATTATTCATGTTAGTTTCTAAAAGGAATCCTACATATTACTTTGTTCAAAATGATTCTTAGTTGCAAGATTTTCACCTTTTTTTTTTTTTTTTAATTTCAGCTTCTTGAAATTATTCAAGAGTTCGACAACAAACAGCAACGAGCATTTCTACAGTTCGTTACCGGCGCGCCGAGGCTTCCTTCTGGTGGCTTTGCATCTCTCAATCCAAAGTTAACCATCGTCCGAAAGGTTTTTATTATCAACAAATTTTACTCAAATGTTATATTCAACTTTGACTTTTTTTACTAACTTCTTTGTTGGGATATTTTTTACACAGCATTCTAGCAATTTGGTCGATTACGACTTACCAAGTGTGATGACCTGTGCAAACTACCTCAAGTTGCCTCCTTACTCTTCAAAGGTAACAACTTTTCTTACAAAATGATCTTGAAATAGTTATGATTCCAAACACGTGATTTCAAGGCATGTTTGGACATAATTCTGAAAGGGTTATGATTTATCCATCATTTTTAAAATCATTCCAAAATATGATTTTAATTATTCAAAACCAATCTTGATGATATCAAAATTGGATTTGAAAGGGTAAAATTCAATACCAAATGAATTCTAATTAATTAAATGCGTATTTCAAAGTGATTGTAACGATTTCAAAATCATTTTCAAACATGCATTTAATCCTTTCAAAATCACTCCCAAACATGTATCAACTTCTCTAATAAACCAATTGCCCTTTCAATTTCCATTAGAACAAAAACATTAACTTTCAAAATTGGTTTCTATCTGTTCAGGAGATAATGAAAGAGAAGCTGTTATATGCTATAACGGAAGGACAGGGCTCGTTTCATCTTTCGTAGCCTTAAATTCCCATCTTCAGTAGAAAGTGAAATTGGTTAATCAATAGAGTAGCTATAGCTGCTGTATAGATTTTTTTCATAAAAAAAAAAAGTAAAAAAAGCAAAAAAAGCAAAGAAAAAAGAAGGGGTAAAAATGGATGAAGAAGTGCAGATTGTGGTGGTTGTGGATAAGGAAGGCTTTCTTCTTCTTCTTCTTCTTCATCTGATTCTGGTGGGTAAAGTTGCAATGTACATAAATCCTATGTACATAGAAAATAGAAGATGGCTGTACAGGAAAATGGTGGGCTTTAGATTTATTGGCATTCGATGGCATCGTCTCTTTTCTCTTTTTCTTTTCTTTTTTTTTTTTTTTTAACCCAATGCTCACATTGTAAATATCTCCTTAGCTAATTAACTTCTAGTTCTCTTTTTTTTGTACATAACATCTTTGTGACTTGGGCTTTGATTTAGTCAATTTTCCGACTAAACCATGCATTTATTTTCTCAGCAGTTGAGAAATAGGCCAAGTTTATTTATTTATTTACTGCATCA

mRNA sequence

CTTTCATAGATATCTTTAAGCTCTGGCGTTCTCTCGCTCTGTTTTTTCATCATCGTCGACGTCAAAAGAGCTTTACAATTTCGATCACCGATAAAAGCATCTGCCGTCAGAGTTGCGTTCCGCCGTCGACGGAGAAGTTTTCTCCCTCACAAACTCCCGTATTTTTTCATCGTGCTTTTGGAATTTCGTGAGTCTTCGGTTTGCAGCGCTAAGGAGGGTGTTTACTCTATTAGAGCTAATAAATGATAAATGGGAAATCGTGGCCAAAAGCGTACTGAAATGGTGGATAGATTACCGGCTGATAAACGAGCATGCAGTTCATTGGAATTCAGACCAAGCTCATCAAATTCATCTATGCAAATGCACGTGACTTCGACCAATTCTAGTCCTGGCATCCATGATAATGACATGGACACTTCATCGTCTGCTTCTGCTTCTAGTCGTTCAGAGGGAGAACATGATAAGGATTCAGCCTACGGGTCTTGTGATTCTGATGATGCTGAACAGAAACATAGTGATCTTCGGAATTATCAGAGACAAAGATCATCAGGTGATCATGGACGATTTAAAAGGCTTTTGACAAGCTTGGGTGAAGAATCTGAGTCATCTGTACAAACAGAACTGCTGAGGGAACTATGTGAGGTTTTATCATTCTGCACGGAGAATTCACTATCGAGTATGACATCAGATTCGTTATCTATAATTCTTGTAAATCTGGTGAAACTTGATAGCGACTCAGATATAGTGCTATTGGCTTTAAGGGCTTTAACTTATTTATGTGATGCTTACCCCAGAGCATCTAGTTTTATAGTTCGTCATGGTGGAGTTCCTGCCTTCTGTAAGAGGCTGGGAGCTATTGAGTACTCGGATGTTGCTGAACAGTGTTTCCAAGCATTGGAAAAATATCACAGGAGCACCCTGTTGCATGCTTAGAGGGTGGTGCAGTTATGGCTGTTTTAACTTTTATTGATTTTTTTCCTACGATCATACAAAGAACGGCTCTTCGTATAGTGGTGAATGTCTGCAAAAAACTTCCTTCCGAGTGTCCACCAAATTTGATTGAGGCTGTTCCTATACTATGCAATCTCCTTCAATATGATGATGAGGAGCTGGTAGAGAATGTGGCTCGATGCATGATTAAAATAGCTGAGTGCGTCCATCAATCCTCCGAGCTTTTGGATGGGCTTTGTCAGCATGGGCTGATTCAACATGCCATTCGCCTTATAAACTTGAATAGCCGGACAACTTTGTCCCAGACCATTTACAATGATTTGCTTGGTGTACTTATCAAACTTGCTTCAGGATCCATTGTTGCTTTTGAAACCCTTTATGAGCTAAACATTAGCAACACTTTGAAGGACATATTGTCTGCTTACAATCTCTCCCATGGGGTGTCTTCTTCGTGTGCTGTAGTTGATGGGCAGCGGAACCAGGTATGTGAAGTCCTGAAATTGCTCAACGAGCTTCTCCCAACTGAAGATGCGAAGACAGAACAATTGTCTGAAAAAGTGTCTTTCTTAGTCGGTAATCCCAAACAACTGCAGAAATTTGGCTTGGATATACTTCCTTTGCTTGTTCAGGTGGTTAGTTCTGGTGCAAACTTGTATGTCTGTTGCGGCTGCTTAACTATTATCTACAAGTTTGTCTGTTTGGGTGAATCCGACATGCTTGTTGAGTTACTTGAGAATTCCAACATTTCTAGTTTCCTGGCTGGAGTGTTTACTCGAAAAGACCACCACGTGCTGATGTTAGGTTTAAAAATCACTGAGATTATCCTGCAAAAGCTCGCTAGTATATTTTTGAAATCATTTGTCAAGGAGGGTGTCTATTTTGCTATTGATGCACTGATATCCCCGGAGAAGTATAAACAGTTGATTTTCCCAGTGTTTACTGGTGTCCACTCATCATTTGATTCCTGTCAAAAGTCTTCAAGAGAACATGGGAAATGCTTATGTTATGCCTTTTCGAGTAGCTGTTTCCCCTCTGGGTCAGAAACAGGCAGCTGCAAGCTTGACAAGGATTCTGTTTATAGTCTTGCAAATCATATAAGAAACATTTATTTTGCTGAAGATTTATGTGACACCGATGAAGGAGTAACTGATATTCTTCAAAATCTTCGAACTTTTTCTGGGGCCCTAGATGATCTACTAAACTTGTCCTTAATTAAGGACACTCCAGCTCAGGATGAAGAAAAATTGTATGCTTTGTTAGCTGAGATTATGTCAAAGCTTAAGTGTGGCGAGCCCATATCCACCTTTGAATTTATTGAAAGTGGAATAGTCAAGTCATTTATAAATTACATAACTAATGGTCAGTATCTGAGGAAAAAGGAAGAAGCACAAACTATATCTAGACAATTCTCAATTATAGAAAGAAGATTTGAGGCTTTTGCAAGGCTACTCTTATCTAGTTCTGACCACCCATCAGTAAATTTACCAGTTTTGGCATTGATAAGGAAATTACAAATCTCACTTTCGTCTTTAGAAAACTTTCCTGTTATTTCAAGCCAGGGATTCAAACATAGGAACTATTTTGCTACGGTTCCAAATGCTCGGTGCGTACCACATCCTTGTGTGAAAGTTCGGTTTGTGAGGGGAGATGGAGAAACAAAACTATGTGACATTACCGGGGATATCCTGACTGTTGACCCGTTTTCTTCTTTAGCTGCCATTGAAGGGTTTCTATGGCGCAAAGTTAGCACAAAAAAAACTGAGCAGTCCCCTGAAGAGACTCTGAGGGAGCATCAAATCAAACTACTATCAAATATACGTTCTTGCTTGGGTGTTAACTCAGAGTTAGTGGGGTCTGACATCATGTCCACAGATTTGCCCGAAGTTCAGGTACCTGCAGAAGTCGGTGCTGATGAGAAATCACAGTCTTCTCCTTCTTGCAGTAAGAAAGGCACGGCTCCCAGATTGCTTCTTTACCTTGAGGGAAAGCAGCTGGAGCCCACCATGACAATTTACCAGGCAATTCTCCAACAACACATCAAAGAAAATGAAACTATATCTGGCACGAAAGTATGGAGCCATGTATATACAATAATGTATAGAAGCGCTGGTGAAGTGGAGGACAACACTTGTAACCAATTGTTTTGTGCATCCGACAAAGCTCCAACATTGCAGTTTTCTTCATTTTTCTGTGACATTCTAGATTGTGTTCTGCCTTCTGACTTGGCAAAAGGAAGTCCGGCTTATGATGTTTTATTTCTTCTAAGGAGCATAGAGGGCATGAACAGGATGGCATTTCACATTATGTCACATGAAAGAATTCGTGCTTTTGCCGAAGGAAAAATTGATACCTTGGACAATATAAAGCTATCAGTTCCAACGGTGTCACAGAATGAATTTGTGAATAGCAAACTGACTGAAAAACTTGAACAGCAGATGAGGGATTCTTCTGCTGTTTCTGTCGGTGGCATGCCTTTATGGTGTAAGGAGCTTATGGATTCATGCCCTTTCTTATTTAGTTTCGAGGCAAGGCGCAAGTACTTTCGGATAGTAGTATTTGGCATGCCACAATACCAGCTACATGTGAGATCACACAGTGACTTGGGGACCTCAAATGACGGACGGTCAAGTTCTGGTGGCTTGCCTCGTAAGAAGGTATTAGTACACCGCGACCAAATTCTAGATTCTGCTTCCAAAATGATGGATCAGTATGCACATCAGAAAGTTCTCCTTGAGGTGGAATATGATGAAGAAGTTGGCACTGGGCTTGGTCCAACATTAGAATTCTATACTCTTGTCAGTCGTGAATTTCAGAAGTATGGCCTTGGAATGTGGAGAGGGGATCATGATGCATTTATATCTGGAAAGAGGCTGAACATTGAGGGTAGAGAAACTACTGAATCTCCATTTGGGCTTTTTCCCCGTCCATGGCCATCAACTCTGGACACAGATAAGCTACATCTTCCCGAAGTAATGAAAAAGTTCGTACTTCTAGGACAAATTGTGGCCAAGGCCATTCAAGATGGTAGGGTATTGGATATCTACTTCTCCAAAAGCTTCTATAAACTAATACTTGGGCAGGAGTTGAGTATTTATGATATCCAATCATTTGATCCAGAGCTCGGTACTGTTCTGCTAGAGTTTCAATCTTTGGTCAACAGAAATAAAATATTGGGAACTGTTTACGAAGAGAACTCATCATCCAAACTCGAATTTTCTTATCATAATACCAATATTGAGGATCTTTGCCTTGATTTTACTCTTCCTGGTTATCCTGATTATTTATTGACTTCGTCTCAAGATAACTCAATGGTAAATGCAAAAAATTTGGAGGATTATGTCTCTCTTGTTGCAGATGCTACTTTATATTCTGGAATTTCAAGACAAATAGAAGCATTTAAGTCTGGATTTAATCAGGTTTTCCCCATAGAACATCTTCAAGTATTTACTGCAGAAGAATTAGAGCGTTTAATATGCGGAGAACAAGACATTTGGGCCTTGAGTGATCTCCTTGACAATATGAAGTTTGACCATGGATATACATCCAGTAGCCCCTCCATTATTCATCTTCTTGAAATTATTCAAGAGTTCGACAACAAACAGCAACGAGCATTTCTACAGTTCGTTACCGGCGCGCCGAGGCTTCCTTCTGGTGGCTTTGCATCTCTCAATCCAAAGTTAACCATCGTCCGAAAGCATTCTAGCAATTTGGTCGATTACGACTTACCAAGTGTGATGACCTGTGCAAACTACCTCAAGTTGCCTCCTTACTCTTCAAAGGAGATAATGAAAGAGAAGCTGTTATATGCTATAACGGAAGGACAGGGCTCGTTTCATCTTTCGTAGCCTTAAATTCCCATCTTCAGTAGAAAGTGAAATTGGTTAATCAATAGAGTAGCTATAGCTGCTGTATAGATTTTTTTCATAAAAAAAAAAAGTAAAAAAAGCAAAAAAAGCAAAGAAAAAAGAAGGGGTAAAAATGGATGAAGAAGTGCAGATTGTGGTGGTTGTGGATAAGGAAGGCTTTCTTCTTCTTCTTCTTCTTCATCTGATTCTGGTGGGTAAAGTTGCAATGTACATAAATCCTATGTACATAGAAAATAGAAGATGGCTGTACAGGAAAATGGTGGGCTTTAGATTTATTGGCATTCGATGGCATCGTCTCTTTTCTCTTTTTCTTTTCTTTTTTTTTTTTTTTTAACCCAATGCTCACATTGTAAATATCTCCTTAGCTAATTAACTTCTAGTTCTCTTTTTTTTGTACATAACATCTTTGTGACTTGGGCTTTGATTTAGTCAATTTTCCGACTAAACCATGCATTTATTTTCTCAGCAGTTGAGAAATAGGCCAAGTTTATTTATTTATTTACTGCATCA

Coding sequence (CDS)

ATGGCTGTTTTAACTTTTATTGATTTTTTTCCTACGATCATACAAAGAACGGCTCTTCGTATAGTGGTGAATGTCTGCAAAAAACTTCCTTCCGAGTGTCCACCAAATTTGATTGAGGCTGTTCCTATACTATGCAATCTCCTTCAATATGATGATGAGGAGCTGGTAGAGAATGTGGCTCGATGCATGATTAAAATAGCTGAGTGCGTCCATCAATCCTCCGAGCTTTTGGATGGGCTTTGTCAGCATGGGCTGATTCAACATGCCATTCGCCTTATAAACTTGAATAGCCGGACAACTTTGTCCCAGACCATTTACAATGATTTGCTTGGTGTACTTATCAAACTTGCTTCAGGATCCATTGTTGCTTTTGAAACCCTTTATGAGCTAAACATTAGCAACACTTTGAAGGACATATTGTCTGCTTACAATCTCTCCCATGGGGTGTCTTCTTCGTGTGCTGTAGTTGATGGGCAGCGGAACCAGGTATGTGAAGTCCTGAAATTGCTCAACGAGCTTCTCCCAACTGAAGATGCGAAGACAGAACAATTGTCTGAAAAAGTGTCTTTCTTAGTCGGTAATCCCAAACAACTGCAGAAATTTGGCTTGGATATACTTCCTTTGCTTGTTCAGGTGGTTAGTTCTGGTGCAAACTTGTATGTCTGTTGCGGCTGCTTAACTATTATCTACAAGTTTGTCTGTTTGGGTGAATCCGACATGCTTGTTGAGTTACTTGAGAATTCCAACATTTCTAGTTTCCTGGCTGGAGTGTTTACTCGAAAAGACCACCACGTGCTGATGTTAGGTTTAAAAATCACTGAGATTATCCTGCAAAAGCTCGCTAGTATATTTTTGAAATCATTTGTCAAGGAGGGTGTCTATTTTGCTATTGATGCACTGATATCCCCGGAGAAGTATAAACAGTTGATTTTCCCAGTGTTTACTGGTGTCCACTCATCATTTGATTCCTGTCAAAAGTCTTCAAGAGAACATGGGAAATGCTTATGTTATGCCTTTTCGAGTAGCTGTTTCCCCTCTGGGTCAGAAACAGGCAGCTGCAAGCTTGACAAGGATTCTGTTTATAGTCTTGCAAATCATATAAGAAACATTTATTTTGCTGAAGATTTATGTGACACCGATGAAGGAGTAACTGATATTCTTCAAAATCTTCGAACTTTTTCTGGGGCCCTAGATGATCTACTAAACTTGTCCTTAATTAAGGACACTCCAGCTCAGGATGAAGAAAAATTGTATGCTTTGTTAGCTGAGATTATGTCAAAGCTTAAGTGTGGCGAGCCCATATCCACCTTTGAATTTATTGAAAGTGGAATAGTCAAGTCATTTATAAATTACATAACTAATGGTCAGTATCTGAGGAAAAAGGAAGAAGCACAAACTATATCTAGACAATTCTCAATTATAGAAAGAAGATTTGAGGCTTTTGCAAGGCTACTCTTATCTAGTTCTGACCACCCATCAGTAAATTTACCAGTTTTGGCATTGATAAGGAAATTACAAATCTCACTTTCGTCTTTAGAAAACTTTCCTGTTATTTCAAGCCAGGGATTCAAACATAGGAACTATTTTGCTACGGTTCCAAATGCTCGGTGCGTACCACATCCTTGTGTGAAAGTTCGGTTTGTGAGGGGAGATGGAGAAACAAAACTATGTGACATTACCGGGGATATCCTGACTGTTGACCCGTTTTCTTCTTTAGCTGCCATTGAAGGGTTTCTATGGCGCAAAGTTAGCACAAAAAAAACTGAGCAGTCCCCTGAAGAGACTCTGAGGGAGCATCAAATCAAACTACTATCAAATATACGTTCTTGCTTGGGTGTTAACTCAGAGTTAGTGGGGTCTGACATCATGTCCACAGATTTGCCCGAAGTTCAGGTACCTGCAGAAGTCGGTGCTGATGAGAAATCACAGTCTTCTCCTTCTTGCAGTAAGAAAGGCACGGCTCCCAGATTGCTTCTTTACCTTGAGGGAAAGCAGCTGGAGCCCACCATGACAATTTACCAGGCAATTCTCCAACAACACATCAAAGAAAATGAAACTATATCTGGCACGAAAGTATGGAGCCATGTATATACAATAATGTATAGAAGCGCTGGTGAAGTGGAGGACAACACTTGTAACCAATTGTTTTGTGCATCCGACAAAGCTCCAACATTGCAGTTTTCTTCATTTTTCTGTGACATTCTAGATTGTGTTCTGCCTTCTGACTTGGCAAAAGGAAGTCCGGCTTATGATGTTTTATTTCTTCTAAGGAGCATAGAGGGCATGAACAGGATGGCATTTCACATTATGTCACATGAAAGAATTCGTGCTTTTGCCGAAGGAAAAATTGATACCTTGGACAATATAAAGCTATCAGTTCCAACGGTGTCACAGAATGAATTTGTGAATAGCAAACTGACTGAAAAACTTGAACAGCAGATGAGGGATTCTTCTGCTGTTTCTGTCGGTGGCATGCCTTTATGGTGTAAGGAGCTTATGGATTCATGCCCTTTCTTATTTAGTTTCGAGGCAAGGCGCAAGTACTTTCGGATAGTAGTATTTGGCATGCCACAATACCAGCTACATGTGAGATCACACAGTGACTTGGGGACCTCAAATGACGGACGGTCAAGTTCTGGTGGCTTGCCTCGTAAGAAGGTATTAGTACACCGCGACCAAATTCTAGATTCTGCTTCCAAAATGATGGATCAGTATGCACATCAGAAAGTTCTCCTTGAGGTGGAATATGATGAAGAAGTTGGCACTGGGCTTGGTCCAACATTAGAATTCTATACTCTTGTCAGTCGTGAATTTCAGAAGTATGGCCTTGGAATGTGGAGAGGGGATCATGATGCATTTATATCTGGAAAGAGGCTGAACATTGAGGGTAGAGAAACTACTGAATCTCCATTTGGGCTTTTTCCCCGTCCATGGCCATCAACTCTGGACACAGATAAGCTACATCTTCCCGAAGTAATGAAAAAGTTCGTACTTCTAGGACAAATTGTGGCCAAGGCCATTCAAGATGGTAGGGTATTGGATATCTACTTCTCCAAAAGCTTCTATAAACTAATACTTGGGCAGGAGTTGAGTATTTATGATATCCAATCATTTGATCCAGAGCTCGGTACTGTTCTGCTAGAGTTTCAATCTTTGGTCAACAGAAATAAAATATTGGGAACTGTTTACGAAGAGAACTCATCATCCAAACTCGAATTTTCTTATCATAATACCAATATTGAGGATCTTTGCCTTGATTTTACTCTTCCTGGTTATCCTGATTATTTATTGACTTCGTCTCAAGATAACTCAATGGTAAATGCAAAAAATTTGGAGGATTATGTCTCTCTTGTTGCAGATGCTACTTTATATTCTGGAATTTCAAGACAAATAGAAGCATTTAAGTCTGGATTTAATCAGGTTTTCCCCATAGAACATCTTCAAGTATTTACTGCAGAAGAATTAGAGCGTTTAATATGCGGAGAACAAGACATTTGGGCCTTGAGTGATCTCCTTGACAATATGAAGTTTGACCATGGATATACATCCAGTAGCCCCTCCATTATTCATCTTCTTGAAATTATTCAAGAGTTCGACAACAAACAGCAACGAGCATTTCTACAGTTCGTTACCGGCGCGCCGAGGCTTCCTTCTGGTGGCTTTGCATCTCTCAATCCAAAGTTAACCATCGTCCGAAAGCATTCTAGCAATTTGGTCGATTACGACTTACCAAGTGTGATGACCTGTGCAAACTACCTCAAGTTGCCTCCTTACTCTTCAAAGGAGATAATGAAAGAGAAGCTGTTATATGCTATAACGGAAGGACAGGGCTCGTTTCATCTTTCGTAG

Protein sequence

MAVLTFIDFFPTIIQRTALRIVVNVCKKLPSECPPNLIEAVPILCNLLQYDDEELVENVARCMIKIAECVHQSSELLDGLCQHGLIQHAIRLINLNSRTTLSQTIYNDLLGVLIKLASGSIVAFETLYELNISNTLKDILSAYNLSHGVSSSCAVVDGQRNQVCEVLKLLNELLPTEDAKTEQLSEKVSFLVGNPKQLQKFGLDILPLLVQVVSSGANLYVCCGCLTIIYKFVCLGESDMLVELLENSNISSFLAGVFTRKDHHVLMLGLKITEIILQKLASIFLKSFVKEGVYFAIDALISPEKYKQLIFPVFTGVHSSFDSCQKSSREHGKCLCYAFSSSCFPSGSETGSCKLDKDSVYSLANHIRNIYFAEDLCDTDEGVTDILQNLRTFSGALDDLLNLSLIKDTPAQDEEKLYALLAEIMSKLKCGEPISTFEFIESGIVKSFINYITNGQYLRKKEEAQTISRQFSIIERRFEAFARLLLSSSDHPSVNLPVLALIRKLQISLSSLENFPVISSQGFKHRNYFATVPNARCVPHPCVKVRFVRGDGETKLCDITGDILTVDPFSSLAAIEGFLWRKVSTKKTEQSPEETLREHQIKLLSNIRSCLGVNSELVGSDIMSTDLPEVQVPAEVGADEKSQSSPSCSKKGTAPRLLLYLEGKQLEPTMTIYQAILQQHIKENETISGTKVWSHVYTIMYRSAGEVEDNTCNQLFCASDKAPTLQFSSFFCDILDCVLPSDLAKGSPAYDVLFLLRSIEGMNRMAFHIMSHERIRAFAEGKIDTLDNIKLSVPTVSQNEFVNSKLTEKLEQQMRDSSAVSVGGMPLWCKELMDSCPFLFSFEARRKYFRIVVFGMPQYQLHVRSHSDLGTSNDGRSSSGGLPRKKVLVHRDQILDSASKMMDQYAHQKVLLEVEYDEEVGTGLGPTLEFYTLVSREFQKYGLGMWRGDHDAFISGKRLNIEGRETTESPFGLFPRPWPSTLDTDKLHLPEVMKKFVLLGQIVAKAIQDGRVLDIYFSKSFYKLILGQELSIYDIQSFDPELGTVLLEFQSLVNRNKILGTVYEENSSSKLEFSYHNTNIEDLCLDFTLPGYPDYLLTSSQDNSMVNAKNLEDYVSLVADATLYSGISRQIEAFKSGFNQVFPIEHLQVFTAEELERLICGEQDIWALSDLLDNMKFDHGYTSSSPSIIHLLEIIQEFDNKQQRAFLQFVTGAPRLPSGGFASLNPKLTIVRKHSSNLVDYDLPSVMTCANYLKLPPYSSKEIMKEKLLYAITEGQGSFHLS
Homology
BLAST of IVF0019533 vs. ExPASy Swiss-Prot
Match: Q9LYZ7 (E3 ubiquitin-protein ligase UPL4 OS=Arabidopsis thaliana OX=3702 GN=UPL4 PE=3 SV=1)

HSP 1 Score: 1206.0 bits (3119), Expect = 0.0e+00
Identity = 654/1297 (50.42%), Postives = 881/1297 (67.93%), Query Frame = 0

Query: 1    MAVLTFIDFFPTIIQRTALRIVVNVCKKLPSECPPNLIEAVPILCNLLQYDDEELVENVA 60
            MAVL+FIDFF T IQR A+  VVN+CK+L SE P   ++AVPILC LLQY+D +LVENVA
Sbjct: 239  MAVLSFIDFFSTSIQRVAISTVVNICKQLSSESPSPFMDAVPILCTLLQYEDRQLVENVA 298

Query: 61   RCMIKIAECVHQSSELLDGLCQHGLIQHAIRLINLNSRTTLSQTIYNDLLGVLIKLASGS 120
             C+ KIA+   +S  +LD LC+HGLI  +  L+NLNSRTTLSQ +YN ++G+L KL+SGS
Sbjct: 299  ICLTKIADQASESPAMLDQLCRHGLINESTHLLNLNSRTTLSQPVYNGVIGMLRKLSSGS 358

Query: 121  IVAFETLYELNISNTLKDILSAYNLSHGVSSSCAVVDGQRNQVCEVLKLLNELLPTEDAK 180
             +AF TLYELNI  +LK+I+S Y++SH VSS+   ++   NQV EVLKL+ ELLP    +
Sbjct: 359  ALAFRTLYELNIGYSLKEIMSTYDISHSVSST-HPINACSNQVHEVLKLVIELLPASPVE 418

Query: 181  TEQL-SEKVSFLVGNPKQLQKFGLDILPLLVQVVSSGANLYVCCGCLTIIYKFVCLGESD 240
              QL SEK SFLV  P  LQ+FG D+LP+++QV++SGAN+YV  GCL+ I+K  CL +S 
Sbjct: 419  DNQLASEKESFLVNQPDLLQQFGRDMLPVMIQVLNSGANVYVSYGCLSAIHKLTCLSKSG 478

Query: 241  MLVELLENSNISSFLAGVFTRKDHHVLMLGLKITEIILQKLASIFLKSFVKEGVYFAIDA 300
             +VELL+N+N+SS LAG+ +RKDHHV+++ L++ E++L+K    FL SF+KEGV+FAI+A
Sbjct: 479  DIVELLKNTNMSSVLAGILSRKDHHVIVVALQVAEVLLEKYRDTFLNSFIKEGVFFAIEA 538

Query: 301  LISPEKYKQLIFPVFTGVHSSFDSCQKS-SREHGKCLCYAFSSSCFPSGSETGSCKLDKD 360
            L+S ++ +Q           S D  QK  ++E  KCLC +F  S     S + +CK++KD
Sbjct: 539  LLSSDRGQQ--------NQGSADLSQKPVTKEIVKCLCQSFERSL---SSSSQTCKIEKD 598

Query: 361  SVYSLANHIRNIYFAEDLCDTDEGVTDILQNLRTFSGALDDLLNLSLIKDTPAQDEEKLY 420
            SVY LA  I+  +F  ++ ++++G+TD+LQNL+  S AL +L+ + +  D     +EK +
Sbjct: 599  SVYVLATRIKEGFFGPEVFNSEKGLTDVLQNLKNLSVALSELMTVPI--DAHVLHDEKFF 658

Query: 421  ALLAEIMSKLKCGEPISTFEFIESGIVKSFINYITNGQYLRKKEEAQTISRQFSIIERRF 480
            ++  +IM +L   E +STFEFIESG+VKS  +Y++NG Y RK  +          I +RF
Sbjct: 659  SIWNQIMERLNGRESVSTFEFIESGVVKSLASYLSNGLYQRKLSKGGPECDSLPFIGKRF 718

Query: 481  EAFARLLLSSSDHPSVNLPVLALIRKLQISLSSLENFPVISSQGFKHRNYFATVPNARCV 540
            E F RLL S  +  S       LI+KLQ SLSSLENFP++ SQ  K +N FA +PN RC 
Sbjct: 719  EVFTRLLWSDGEATS-----SLLIQKLQNSLSSLENFPIVLSQFLKQKNSFAAIPNGRCT 778

Query: 541  PHPCVKVRFVRGDGETKLCDITGDILTVDPFSSLAAIEGFLWRKVSTKKTE--QSPEETL 600
             +PC+KVRF++ +GET L D + D +TVDP   L A++ +LW KV+ +  +  ++ ++ +
Sbjct: 779  SYPCLKVRFLKAEGETSLRDYSQDFVTVDPLCYLDAVDQYLWPKVNIEPIDSVEAKDQAI 838

Query: 601  REHQIKLLSNIRSCLGVNSELVGSDIMSTDLPEV---QVPAEV---GADEKSQSSPSCSK 660
                 +L S   SC   +S  +  D  S+D  ++   QV  +    G    S S  S  K
Sbjct: 839  ECQSSQLQSTSISCQAESSSPMEIDSESSDASQLQGSQVEDQTQLPGQQNASSSETSSEK 898

Query: 661  KGTAPRLLLYLEGKQLEPTMTIYQAILQQHIKENETISGTKVWSHVYTIMYRSAGEVEDN 720
            +   PRLL  LEG +L+ ++T+YQAIL   +K     +     S  + I Y  + ++ D+
Sbjct: 899  EDAVPRLLFRLEGLELDRSLTVYQAILLHKLKSESEATNDSKLSGPHNITYERSAQLGDS 958

Query: 721  TCNQLFCASDKAPTLQFSSFFCDILDCVLPSDLA---KGS--PAYDVLFLLRSIEGMNRM 780
              N LF      P       +   L  +    LA   KGS  P YD+LFLL+S+EGMNR 
Sbjct: 959  REN-LF-----PPGSMEDDEYRPFLSYLFTHRLALRLKGSSHPPYDILFLLKSLEGMNRF 1018

Query: 781  AFHIMSHERIRAFAEGKIDTLDNIKLSVPTVSQNEFVNSKLTEKLEQQMRDSSAVSVGGM 840
             FH++S ERI AF EG+++ LD++++ V  V  +EFV+SKLTEKLEQQ+RDS AVS  G+
Sbjct: 1019 LFHLISLERINAFGEGRLENLDDLRVQVRPVPHSEFVSSKLTEKLEQQLRDSFAVSTCGL 1078

Query: 841  PLWCKELMDSCPFLFSFEARRKYFRIVVFGMPQYQLHVRSHSDLGTSNDGRSSSGGLPRK 900
            P W  +LMDSCP LFSFEA+ KYFR+  FG  + + H +  S      + R  +G LPRK
Sbjct: 1079 PPWFNDLMDSCPCLFSFEAKSKYFRLAAFGSQKIRHHPQHLSSSNVHGEARPVTGSLPRK 1138

Query: 901  KVLVHRDQILDSASKMMDQYAHQKVLLEVEYDEEVGTGLGPTLEFYTLVSREFQKYGLGM 960
            K L  R+ IL+SA+KMM+ Y +QKV++EVEY EEVGTGLGPTLEFYTLVSR FQ   LGM
Sbjct: 1139 KFLACRENILESAAKMMELYGNQKVVIEVEYSEEVGTGLGPTLEFYTLVSRAFQNPDLGM 1198

Query: 961  WRGDHDAFISGKRLNIEGRETTESPFGLFPRPWPSTLDTDKLHLPEVMKKFVLLGQIVAK 1020
            WR D  +FI GK +   G     S  GLFPRPW  T  T      +V++KFVLLG +VAK
Sbjct: 1199 WRND-CSFIVGKPVEHSG--VLASSSGLFPRPWSGTSTTS-----DVLQKFVLLGTVVAK 1258

Query: 1021 AIQDGRVLDIYFSKSFYKLILGQELSIYDIQSFDPELGTVLLEFQSLVNRNKILGTVYEE 1080
            A+QDGRVLD+  SK+FYKLILGQELS +DI   DPEL   L+E Q+LV R K+    + +
Sbjct: 1259 ALQDGRVLDLPLSKAFYKLILGQELSSFDIHFVDPELCKTLVELQALVRRKKLFAEAHGD 1318

Query: 1081 NSSSKLEFSYHNTNIEDLCLDFTLPGYPDYLLTSSQDNSMVNAKNLEDYVSLVADATLYS 1140
            + ++K + S+H T IEDLCL+F LPGY DY L     N MVN  NLE+Y+  + +AT+ +
Sbjct: 1319 SGAAKCDLSFHGTKIEDLCLEFALPGYTDYDLAPYSANDMVNLDNLEEYIKGIVNATVCN 1378

Query: 1141 GISRQIEAFKSGFNQVFPIEHLQVFTAEELERLICGEQDIWALSDLLDNMKFDHGYTSSS 1200
            GI +Q+EAF+SGFNQVF IEHL++F  EELE ++CGE D+++++++LD++KFDHGYTSSS
Sbjct: 1379 GIQKQVEAFRSGFNQVFSIEHLRIFNEEELETMLCGECDLFSMNEVLDHIKFDHGYTSSS 1438

Query: 1201 PSIIHLLEIIQEFDNKQQRAFLQFVTGAPRLPSGGFASLNPKLTIVRKHSSNLVDYDLPS 1260
            P + +LL+I+ EFD +QQRAFLQFVTG+PRLP GG ASL+PKLTIVRKH S+  D DLPS
Sbjct: 1439 PPVEYLLQILHEFDREQQRAFLQFVTGSPRLPHGGLASLSPKLTIVRKHGSDSSDTDLPS 1498

Query: 1261 VMTCANYLKLPPYSSKEIMKEKLLYAITEGQGSFHLS 1283
            VMTCANYLKLPPYSSKE MKEKL+YAITEGQGSFHLS
Sbjct: 1499 VMTCANYLKLPPYSSKEKMKEKLIYAITEGQGSFHLS 1502

BLAST of IVF0019533 vs. ExPASy Swiss-Prot
Match: Q6WWW4 (E3 ubiquitin-protein ligase UPL3 OS=Arabidopsis thaliana OX=3702 GN=UPL3 PE=1 SV=1)

HSP 1 Score: 802.7 bits (2072), Expect = 5.9e-231
Identity = 557/1574 (35.39%), Postives = 805/1574 (51.14%), Query Frame = 0

Query: 1    MAVLTFIDFFPTIIQRTALRIVVNVCKKLPSECPPNLIEAVPILCNLLQYDDEELVENVA 60
            MAVL+++DFF T +QR AL    N+CKKLPS+    ++EAVP+L NLLQY D +++E  +
Sbjct: 323  MAVLSYLDFFSTGVQRVALSTAANMCKKLPSDASDYVMEAVPLLTNLLQYHDSKVLEYAS 382

Query: 61   RCMIKIAECVHQSSELLDGLCQHGLIQHAIRLINLNS----RTTLSQTIYNDLLGVLIKL 120
             C+ +IAE      E LD LC HGL+  A  LI+ ++    + +LS + Y  L+ +L   
Sbjct: 383  ICLTRIAEAFAPYPEKLDELCNHGLVTQAASLISTSNSGGGQASLSVSTYTGLIRLLSTC 442

Query: 121  ASGSIVAFETLYELNISNTLKDILSAYNLSHGVSSSCAV---VDGQRNQVCEVLKLLNEL 180
            ASGS + F TL  L IS+ LKDIL    L  GVS++ +V   +    +Q+ E++ L NEL
Sbjct: 443  ASGSPLGFRTLLLLGISSILKDIL----LGSGVSANASVSPALSRPADQIYEIVNLANEL 502

Query: 181  L---------------------------PTEDAKTE---QLSEKVSFLVGNPKQLQKFGL 240
            L                           P+   K E   ++S +   L   P+ LQ+FGL
Sbjct: 503  LPPLPEGVISLPTSTNALVKGSCQKKSSPSTSGKQEDILKISPREKLLGDQPELLQQFGL 562

Query: 241  DILPLLVQVVSSGANLYVCCGCLTIIYKFVCLGESDMLVELLENSNISSFLAGVFTRKDH 300
            D+LP+LVQ+  S  N  +   CL++I K +    S+M+  L+ ++NISSFLAGV   KD 
Sbjct: 563  DLLPVLVQIYGSSVNGTIRHKCLSVIGKLMYFSSSEMIQSLIGDTNISSFLAGVLAWKDP 622

Query: 301  HVLMLGLKITEIILQKLASIFLKSFVKEGVYFAIDALI--------SPEKYKQLIFP--- 360
             VL+  L++ EI+++KL   F K FV+EGV  A+D L+        SP        P   
Sbjct: 623  QVLVPALQVAEILMEKLPETFSKVFVREGVVHAVDQLVLVGKPSHASPTDKDNDCVPGSA 682

Query: 361  ---VFTGVHSSFDSCQKSSREHGKCLCYAFSSSCFPSGSETGSCKLDKDSVYSLANHIRN 420
                +    S+ +S    S E          ++     + T S  L +++V S A   ++
Sbjct: 683  RSRRYRRRSSNANSDGNQSEEPKNPASLTIGANHNSLDTPTASFML-RETVSSCAKAFKD 742

Query: 421  IYFAEDLCDTDEGVTDILQNLRT----FSGALDD---------LLNLSLIKDTPAQDEEK 480
             YF  D  D D GVTD L +L+      +  +DD           +   + D  A  EE 
Sbjct: 743  KYFPSDGGDVDVGVTDDLLHLKNLCTKLTAGIDDHKVKGKGKSKASGPFLGDFSASKEEY 802

Query: 481  LYALLAEIMSKLKCGEPISTFEFIESGIVKSFINYITNGQYLRKKEEAQTISRQFSIIER 540
            L  +++EI+ ++  G+ +STFEFI SG+V + +NY + G + ++K     + +      R
Sbjct: 803  LIGVISEILGEISKGDGVSTFEFIGSGVVAALLNYFSCGYFSKEKISELNLPKLRQEGLR 862

Query: 541  RFEAFARLLLSSSDHPSVNLPVLALIRKLQISLSSLENFPVISSQGFKHRNYFATVPNA- 600
            RF+AF  + L    +     P+  LI+KLQ +LSSLE FPV+ S   +  +  A + +  
Sbjct: 863  RFKAFLEVALPFDGNEGKVPPMTVLIQKLQNALSSLERFPVVLSHPSRSLSGSARLSSGL 922

Query: 601  RCVPHPCVKVRFVRGDGETKLCDITGDILTVDPFSSLAAIEGFLWRKVS----------- 660
              + HP +K+R  R  GE  L D + +I+ +DP +SLAA+E FLW +V            
Sbjct: 923  SALAHP-LKLRLCRASGEKTLRDYSSNIVLIDPLASLAAVEEFLWPRVQRSESALKPAAP 982

Query: 661  ---------------TKKTEQSPEETLREHQIKLLSNIR--------------------- 720
                           +  +  +P  T R H  +  S I                      
Sbjct: 983  IGNTEPGTLPSGAGVSSPSSSTPASTTRRHSSRSRSAINIGDTSKKDPVHEKGTSSSKGK 1042

Query: 721  ------------------------------------------------SCLGVNSELV-- 780
                                                            S + ++  LV  
Sbjct: 1043 GKGVMKPAQADKGPQTRSNAQKRAVLDKDTQMKPASGDSSSEDEELEISPVDIDDALVIE 1102

Query: 781  -----------GSDIMSTDLP--------EVQVPAEVGAD-----EKSQSSPSCSKKGTA 840
                         D++   LP        +V++   V  D      +  +  S    G A
Sbjct: 1103 EDDISDDEDDDNEDVLDDSLPMCTPDKVHDVKLADSVDDDGLATSGRQMNPASGGTSGAA 1162

Query: 841  ----------------------------------------------------------PR 900
                                                                       +
Sbjct: 1163 AARASDSIDTGIGNSYGSRGALSFAAAAMAGLGAASGRGIRGSRDLHGRTLNRSSDEPSK 1222

Query: 901  LLLYLEGKQLEPTMTIYQAILQQHI---KENETISGTKV-------WSHVYTIMYR---- 960
            L+    GKQL   +TIYQA+ +Q +    +++   G+ +       ++ +YTIMY+    
Sbjct: 1223 LIFTAAGKQLSRHLTIYQAVQRQLMLDEDDDDRFGGSDLVSSDGSRFNDIYTIMYQRPDS 1282

Query: 961  -----SAGEVEDNTCNQLFCASDKAPTLQFSSFFCDILDCV----LPSDLAKGSPAYDVL 1020
                 S G     T ++   ++    +++  S    +LD +    LP DL K +  Y+VL
Sbjct: 1283 QVNRLSVGGASSTTPSKSTKSATTNSSVESQSHRASLLDSILQGELPCDLEKSNSTYNVL 1342

Query: 1021 FLLRSIEGMNRMAFHIMSHERIRAFAEGKIDTLDNIKLSVPTVSQNEFVNSKLTEKLEQQ 1080
             LLR +EG+N++   + +      FAEGKI +LD++  +   V  +EFVNSKLT KL +Q
Sbjct: 1343 ALLRVLEGLNQLCPRLRAQTLSDRFAEGKITSLDDLSTTAAKVPLDEFVNSKLTPKLARQ 1402

Query: 1081 MRDSSAVSVGGMPLWCKELMDSCPFLFSFEARRKYFRIVVFGMPQ--YQLHVRSHSD-LG 1140
            ++D+ A+  G +P WC +L  +CPFLF F+ RR+YF    FG+ +   +L  +  +D  G
Sbjct: 1403 IQDALALCSGSLPSWCYQLTRACPFLFPFQTRRQYFYSTAFGLSRALNRLQQQQGADGSG 1462

Query: 1141 TSNDGRSSSGGLPRKKVLVHRDQILDSASKMMDQYAHQKVLLEVEYDEEVGTGLGPTLEF 1200
            ++N+     G L R+KV V R++ILDSA+K+M+ Y+ QK +LEVEY  EVGTGLGPTLEF
Sbjct: 1463 STNEREMRIGRLQRQKVRVSRNRILDSAAKVMEMYSSQKAVLEVEYFGEVGTGLGPTLEF 1522

Query: 1201 YTLVSREFQKYGLGMWR---GDHDAFISGK------RLNIEGRETTESPFGLFPRPWPST 1260
            YTL+S + QK  LGMWR   GD  +   G+      + +   R+   +P GLFPRPWPST
Sbjct: 1523 YTLLSHDLQKASLGMWRSSSGDKVSMQIGRDEIEDGKPSAANRDIVLAPLGLFPRPWPST 1582

Query: 1261 LD-TDKLHLPEVMKKFVLLGQIVAKAIQDGRVLDIYFSKSFYKLILGQELSIYDIQSFDP 1283
             D ++     +V++ F LLG+++AKA+QDGR+LD+  S +FYKLILGQEL ++DI  FD 
Sbjct: 1583 ADISEGGQFHKVIEYFRLLGRVMAKALQDGRLLDVPLSTAFYKLILGQELDLHDIVLFDA 1642

BLAST of IVF0019533 vs. ExPASy Swiss-Prot
Match: F1RCR6 (E3 ubiquitin-protein ligase TRIP12 OS=Danio rerio OX=7955 GN=trip12 PE=3 SV=1)

HSP 1 Score: 439.9 bits (1130), Expect = 1.0e-121
Identity = 407/1511 (26.94%), Postives = 648/1511 (42.89%), Query Frame = 0

Query: 4    LTFIDFFPTIIQRTALRIVVNVCKKLPSECPPNLIEAVPILCNLLQYDDEELVENVARCM 63
            L +++FF    QR AL I  N C+ +  +    + +++P+L   L + D++ VE+   C 
Sbjct: 607  LLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCF 666

Query: 64   IKIAECVHQSSELLDGLCQHGLIQHAIRLINLNSRTTLSQTIYNDLLGVLIKLASGSIVA 123
             ++ +       LL  +    L+ +  +L+ L +   LS  ++  ++ +   + S     
Sbjct: 667  ARLVDNFQHEENLLQQVASRDLLTNIQQLLVL-TPPVLSSGMFIMVVRMFSLMCSNCPCL 726

Query: 124  FETLYELNISNTLKDILSAYNLSHGVSSSCAVVDGQRNQVCEVLKLLNELLP-------- 183
               L + NI+ TL+ +L   + +        +V     ++ E+  L+ EL+P        
Sbjct: 727  AVQLMKQNIAETLRFLLCGAS-NGSCQEQIDLVPRSPQELYELTSLICELMPCLPREGIF 786

Query: 184  ------------------------------------------TEDAKTEQLSEKVSFLVG 243
                                                       ED  T +  ++    + 
Sbjct: 787  AVDAMLKKGSAQTTEGAIWQWRDDRGLWHPYNRIDSRIIEQINEDTGTARAIQRKPNPLA 846

Query: 244  NPK--------------QLQK--------FGLDILPLLVQVVSSGANLYVCCGCLTIIYK 303
            NP               QL K        F   +  +L +V SS A   V   CL  I +
Sbjct: 847  NPNTGGHLEVRREDARAQLMKEDPELAKCFIKTLFGVLYEVYSSSAGPAVRHKCLRAILR 906

Query: 304  FVCLGESDMLVELLENSNISSFLAGVFTRKDHHVLMLGLKITEIILQKLASIFLKSFVKE 363
             +   ++++L ++L N  +SS +A + + +D  +++  L++ EI++QKL  +F   F +E
Sbjct: 907  IIYFADAELLKDVLRNHAVSSHIASMLSSQDLKIVVGSLQMAEILMQKLPDVFSVYFRRE 966

Query: 364  GVYFAIDALISPEKY--------------------------------KQLIFPVFTGVHS 423
            GV   +  L   E +                                  L  P F   HS
Sbjct: 967  GVMHQVKNLAESEVFLTSPPKACTSGTASLCTTTITTATTTAASNVTPDLGSPSFQ--HS 1026

Query: 424  SFDSCQ------------------------------------------------KSS--- 483
              DS                                                  KSS   
Sbjct: 1027 MDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLA 1086

Query: 484  ----REHGKCLCYAFSSSCFPS--GSETGSCKL-DKDSVYSLANHIR-----------NI 543
                +  GK    A S++  PS     +G  ++  KDSV +  + I+             
Sbjct: 1087 SLNPKTWGKLGTQANSANSEPSRTAGVSGLARVPPKDSVSNNRDKIKAWIKEQASKFVER 1146

Query: 544  YFAEDLCDTDEGVTDILQNLRTFSGALDDLLNLSLIKDTPAQDEEKLYALLAEIMSKLKC 603
            YF  +  D      ++LQ L T +    + LNL +  D+  +  E++ ++++E       
Sbjct: 1147 YFNSESVDGSNPALNVLQRLCTAT----EQLNLQM--DSGVECLEEISSIVSE------- 1206

Query: 604  GEPISTFEFIESGIVKSFINYITNGQYLRKKEEAQTISRQFSIIERRFEAFARLLLSSSD 663
               +S+FE   SG+VK  + Y+T+        E  TISR    I+R    F    +   +
Sbjct: 1207 -SDVSSFEIQHSGLVKQLLLYLTS------NSERDTISRD-ERIKRFLHVFFGCPIPGQE 1266

Query: 664  -----HPSVNLPVLALIRKLQISLSSLENFPV----------ISSQGFKHRNYFATVPNA 723
                  P+ N P+LAL+ K+   LS +E FPV            S+G +   +F T    
Sbjct: 1267 PPGRLDPTENGPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGNGSRGSQALKFFNT---- 1326

Query: 724  RCVPHPCVKVRFVRGDGETKLCDITGDILTVDPFSSLAAIEGFLWRKVSTKKTEQSPEET 783
                H  +K +  R    T +    G  + +DP + + AIE +L  +   +  E+  +  
Sbjct: 1327 ----HQ-LKCQLQRHPDCTNVKQWKGGPVKIDPLALVQAIERYLVVRGYGRIREEDED-- 1386

Query: 784  LREHQIKLLSNIRSCLGVNSELVGSDIMSTDLPEVQVPAEVGADEKSQSSPSCSKKGTAP 843
                               S+  GSD       E+         ++S ++   +      
Sbjct: 1387 -------------------SDDDGSD------DEI---------DESLAAQFLNSGSVRH 1446

Query: 844  RLLLYLEGKQLEPTMTIYQAILQQHIKENETISGTK----------VWSHVYTIMYRSAG 903
            RL  Y+    L   MT+YQA+ Q  ++  E    T           +W+  +T+ Y+   
Sbjct: 1447 RLQFYIGEHLLPYNMTVYQAVRQFSLQAEEERESTDDEANPLGRAGIWTKTHTVWYKPVR 1506

Query: 904  EVEDNTCNQLFCASDKAPTL----------------------QFSSFFCDILDCVLPSDL 963
            E E+   + +     +A T                         ++     L C  P  +
Sbjct: 1507 EDEEGCKDAVGGKRGRAQTAPTKTSPRNAKKQDELWHEGVCPSVANPLETYLICDPPEGI 1566

Query: 964  AKGSPAYDVLFLLRSIEGMNRMAFHIMSHERIRAFAEGKIDTLDNIKLSVPTVSQNEFVN 1023
                P+ +V+ LLR +  ++R  F++  +   +                +PT    EF+N
Sbjct: 1567 TFDDPSMEVILLLRVLHSISRYWFYLYDNAACKEI--------------IPT---GEFIN 1626

Query: 1024 SKLTEKLEQQMRDSSAVSVGGMPLWCKELMDSCPFLFSFEARRKYFRIVVFGMP---QYQ 1083
            SKLT K  +Q++D   +  G +P W  EL  +CPF F F+ R+  F +  F      Q  
Sbjct: 1627 SKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRL 1686

Query: 1084 LHVRSHSDLGTSNDGRSSSGGLPRKKVLVHRDQILDSASKMMDQYAHQKVLLEVEYDEEV 1143
            L      +   S D R +   L RKK  ++RD++L  A  +M      + +LE++Y+ EV
Sbjct: 1687 LDTNPEINQSDSQDSRVAP-RLDRKKRTINRDELLKQAESVMQDLGSSRAMLEIQYENEV 1746

Query: 1144 GTGLGPTLEFYTLVSREFQKYGLGMWRGDHDAFISGKRLNIEGRETTESPFGLFPRPWPS 1203
            GTGLGPT EFY LVS+E Q+  LG+WRG+ +  +S  + + EG +   S  GLF  P+  
Sbjct: 1747 GTGLGPTQEFYALVSQELQRADLGLWRGE-EVTLSNPKGSQEGTKYMFSSRGLFAVPFGR 1806

Query: 1204 TLDTDKLHLPEVMKKFVLLGQIVAKAIQDGRVLDIYFSKSFYKLILGQELSI--YDIQSF 1263
            T  T   H+ ++  KF  LG+++AKAI D R+LD+     FYK +L  E SI  +D+ + 
Sbjct: 1807 T--TKPAHIAKIKMKFRFLGKLMAKAIMDFRLLDLPLGLPFYKWMLRHESSISSHDLVNI 1866

Query: 1264 DPELGTVLLEFQSLVNRNKIL---GTVYEENSSSKLE-FSYHNTNIEDLCLDFTLPGYPD 1283
            DP +   +   + ++ + K +    +   E     LE  + +  ++EDL LDFTLPG+P+
Sbjct: 1867 DPGVAKSIQHLEDIIRQKKRIEQDRSHTRETLQQALESLNMNGCSVEDLGLDFTLPGFPN 1926

BLAST of IVF0019533 vs. ExPASy Swiss-Prot
Match: Q14669 (E3 ubiquitin-protein ligase TRIP12 OS=Homo sapiens OX=9606 GN=TRIP12 PE=1 SV=1)

HSP 1 Score: 435.6 bits (1119), Expect = 1.9e-120
Identity = 406/1530 (26.54%), Postives = 649/1530 (42.42%), Query Frame = 0

Query: 4    LTFIDFFPTIIQRTALRIVVNVCKKLPSECPPNLIEAVPILCNLLQYDDEELVENVARCM 63
            L +++FF    QR AL I  N C+ +  +    + +++P+L   L + D++ VE+   C 
Sbjct: 567  LLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCF 626

Query: 64   IKIAECVHQSSELLDGLCQHGLIQHAIRLINLNSRTTLSQTIYNDLLGVLIKLASGSIVA 123
             ++ +       LL  +    L+ +  +L+ + +   LS  ++  ++ +   + S     
Sbjct: 627  ARLVDNFQHEENLLQQVASKDLLTNVQQLL-VVTPPILSSGMFIMVVRMFSLMCSNCPTL 686

Query: 124  FETLYELNISNTLKDILSAYNLSHGVSSSCAVVDGQRNQVCEVLKLLNELLP-------- 183
               L + NI+ TL  +L   + +        +V     ++ E+  L+ EL+P        
Sbjct: 687  AVQLMKQNIAETLHFLLCGAS-NGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIF 746

Query: 184  ---------------------------------TEDAKTEQLSE---------------- 243
                                              +    EQ++E                
Sbjct: 747  AVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEQINEDTGTARAIQRKPNPLA 806

Query: 244  ---------------KVSFLVGNPKQLQKFGLDILPLLVQVVSSGANLYVCCGCLTIIYK 303
                           +   +  +P+  + F   +  +L +V SS A   V   CL  I +
Sbjct: 807  NSNTSGYSESKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILR 866

Query: 304  FVCLGESDMLVELLENSNISSFLAGVFTRKDHHVLMLGLKITEIILQKLASIFLKSFVKE 363
             +   ++++L ++L+N  +SS +A + + +D  +++  L++ EI++QKL  IF   F +E
Sbjct: 867  IIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRRE 926

Query: 364  GVYFAIDAL--------------------------------------------------- 423
            GV   +  L                                                   
Sbjct: 927  GVMHQVKHLAESESLLTSPPKACTNGSGSMGSTTSVSSGTATAATHAAADLGSPSLQHSR 986

Query: 424  -----ISPE--------------------KY----------KQLIFPVFTGVHSSFDSCQ 483
                 +SP+                    KY           Q   P  T    S     
Sbjct: 987  DDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLAS 1046

Query: 484  KSSREHGKCLCYAFSSSCFPSGSETGSCKLDKDSVYSLANHIRNI--------------Y 543
             + +  G+    + S++  P+ +  GS      S  +++N+   I              Y
Sbjct: 1047 LNPKTWGRLSTQSNSNNIEPARTAGGSGLARAASKDTISNNREKIKGWIKEQAHKFVERY 1106

Query: 544  FAEDLCDTDEGVTDILQNLRTFSGALDDLLNLSLIKDTPAQDEEKLYALLAEIMSKLKCG 603
            F+ +  D      ++LQ L     A  + LNL +  D  A+   ++ ++++E        
Sbjct: 1107 FSSENMDGSNPALNVLQRL----CAATEQLNLQV--DGGAECLVEIRSIVSE-------- 1166

Query: 604  EPISTFEFIESGIVKSFINYITNGQYLRKKEEAQTISRQFSIIERRFEAFARLLLSS--- 663
              +S+FE   SG VK  + Y+T+      K E   +SR     E R + F  +  SS   
Sbjct: 1167 SDVSSFEIQHSGFVKQLLLYLTS------KSEKDAVSR-----EIRLKRFLHVFFSSPLP 1226

Query: 664  ------SDHPSVNLPVLALIRKLQISLSSLENFPV---------------ISSQGFKHRN 723
                     P  N P+LAL+ K+   LS +E FPV                 ++G +   
Sbjct: 1227 GEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALK 1286

Query: 724  YFATVP-NARCVPHP-CVKVRFVRGDGETKLCDITGDILTVDPFSSLAAIEGFLWRKVST 783
            +F T     +   HP C  V+  +G G  K          +DP + + AIE +L      
Sbjct: 1287 FFNTHQLKCQLQRHPDCANVKQWKG-GPVK----------IDPLALVQAIERYL------ 1346

Query: 784  KKTEQSPEETLREHQIKLLSNIRSCLGVNSELVGSDIMSTDLPEVQVPAEVGADEKSQSS 843
                                 +R    V  +   SD             + G+DE+   S
Sbjct: 1347 --------------------VVRGYGRVREDDEDSD-------------DDGSDEEIDES 1406

Query: 844  PSCS--KKGTAPRLLLYLEGKQLEP-TMTIYQAILQQHIK----------ENETISGTKV 903
             +      G     L +  G+ L P  MT+YQA+ Q  I+          E+  +    +
Sbjct: 1407 LAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGI 1466

Query: 904  WSHVYTIMYRSAGEVEDNTCNQLFCASDK------APTLQ-----------FSSFFCDIL 963
            W+  +TI Y+   E E++  +   C   K      APT             +    C  +
Sbjct: 1467 WTKTHTIWYKPVREDEESNKD---CVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSV 1526

Query: 964  DCVL--------PSDLAKGSPAYDVLFLLRSIEGMNRMAFHIMSHERIRAFAEGKIDTLD 1023
               L        P ++    P+ DV+ LLR +  ++R  +++  +   +           
Sbjct: 1527 SNPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEI--------- 1586

Query: 1024 NIKLSVPTVSQNEFVNSKLTEKLEQQMRDSSAVSVGGMPLWCKELMDSCPFLFSFEARRK 1083
                 +PT   +EF+NSKLT K  +Q++D   +  G +P W  EL  +CPF F F+ R+ 
Sbjct: 1587 -----IPT---SEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQM 1646

Query: 1084 YFRIVVFGMP---QYQLHVRSHSDLGTSNDGRSSSGGLPRKKVLVHRDQILDSASKMMDQ 1143
             F +  F      Q  L      +   S D R +   L RKK  V+R+++L  A  +M  
Sbjct: 1647 LFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAP-RLDRKKRTVNREELLKQAESVMQD 1706

Query: 1144 YAHQKVLLEVEYDEEVGTGLGPTLEFYTLVSREFQKYGLGMWRGDHDAFISGKRLNIEGR 1203
                + +LE++Y+ EVGTGLGPTLEFY LVS+E Q+  LG+WRG+ +  +S  + + EG 
Sbjct: 1707 LGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGE-EVTLSNPKGSQEGT 1766

Query: 1204 ETTESPFGLFPRPWPSTLDTDKLHLPEVMKKFVLLGQIVAKAIQDGRVLDIYFSKSFYKL 1263
            +  ++  GLF  P+  T      H+ +V  KF  LG+++AKAI D R++D+     FYK 
Sbjct: 1767 KYIQNLQGLFALPFGRT--AKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKW 1826

Query: 1264 ILGQELSI--YDIQSFDPELGTVLLEFQSLVNRNKILGTVYEENSSSKLEFSY------- 1283
            +L QE S+  +D+   DP +   +   + +V + K L    ++ S +K    Y       
Sbjct: 1827 MLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRL---EQDKSQTKESLQYALETLTM 1886

BLAST of IVF0019533 vs. ExPASy Swiss-Prot
Match: E1B7Q7 (E3 ubiquitin-protein ligase TRIP12 OS=Bos taurus OX=9913 GN=TRIP12 PE=2 SV=2)

HSP 1 Score: 433.7 bits (1114), Expect = 7.1e-120
Identity = 405/1530 (26.47%), Postives = 649/1530 (42.42%), Query Frame = 0

Query: 4    LTFIDFFPTIIQRTALRIVVNVCKKLPSECPPNLIEAVPILCNLLQYDDEELVENVARCM 63
            L +++FF    QR AL I  N C+ +  +    + +++P+L   L + D++ VE+   C 
Sbjct: 567  LLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCF 626

Query: 64   IKIAECVHQSSELLDGLCQHGLIQHAIRLINLNSRTTLSQTIYNDLLGVLIKLASGSIVA 123
             ++ +       LL  +    L+ +  +L+ + +   LS  ++  ++ +   + S     
Sbjct: 627  ARLVDNFQHEENLLQQVASKDLLTNVQQLL-VVTPPILSSGMFIMVVRMFSLMCSNCPTL 686

Query: 124  FETLYELNISNTLKDILSAYNLSHGVSSSCAVVDGQRNQVCEVLKLLNELLP-------- 183
               L + NI+ TL  +L   + +        +V     ++ E+  L+ EL+P        
Sbjct: 687  AVQLMKQNIAETLHFLLCGAS-NGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIF 746

Query: 184  ---------------------------------TEDAKTEQLSE---------------- 243
                                              +    EQ++E                
Sbjct: 747  AVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEQINEDTGTARAIQRKPNPLA 806

Query: 244  ---------------KVSFLVGNPKQLQKFGLDILPLLVQVVSSGANLYVCCGCLTIIYK 303
                           +   +  +P+  + F   +  +L +V SS A   V   CL  I +
Sbjct: 807  NTNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILR 866

Query: 304  FVCLGESDMLVELLENSNISSFLAGVFTRKDHHVLMLGLKITEIILQKLASIFLKSFVKE 363
             +   ++++L ++L+N  +SS +A + + +D  +++  L++ EI++QKL  IF   F +E
Sbjct: 867  IIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRRE 926

Query: 364  GVYFAIDAL--------------------------------------------------- 423
            GV   +  L                                                   
Sbjct: 927  GVMHQVKHLAESESLLTSPPKACTNGSGSLGSTPSVNSGTATAATNASADLGSPSLQHSR 986

Query: 424  -----ISPE--------------------KY----------KQLIFPVFTGVHSSFDSCQ 483
                 +SP+                    KY           Q   P  T    S     
Sbjct: 987  DDSLDLSPQGRLSDVLKRKRLPKRGSRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLAS 1046

Query: 484  KSSREHGKCLCYAFSSSCFPSGSETGSCKLDKDSVYSLANHIRNI--------------Y 543
             + +  G+    + S++  P+ +   S      S  +++N+   I              Y
Sbjct: 1047 LNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERY 1106

Query: 544  FAEDLCDTDEGVTDILQNLRTFSGALDDLLNLSLIKDTPAQDEEKLYALLAEIMSKLKCG 603
            F+ +  D      ++LQ L     A  + LNL +  D  A+   ++ ++++E        
Sbjct: 1107 FSSENMDGSNPALNVLQRL----CAATEQLNLQV--DGGAECLVEIRSIVSE-------- 1166

Query: 604  EPISTFEFIESGIVKSFINYITNGQYLRKKEEAQTISRQFSIIERRFEAFARLLLSS--- 663
              +S+FE   SG VK  + Y+T+      K E   +SR     E R + F  +  SS   
Sbjct: 1167 SDVSSFEIQHSGFVKQLLLYLTS------KSEKDAVSR-----EIRLKRFLHVFFSSPLP 1226

Query: 664  ------SDHPSVNLPVLALIRKLQISLSSLENFPV---------------ISSQGFKHRN 723
                     P  N P+LAL+ K+   LS +E FPV                 ++G +   
Sbjct: 1227 GEEPIERVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALK 1286

Query: 724  YFATVP-NARCVPHP-CVKVRFVRGDGETKLCDITGDILTVDPFSSLAAIEGFLWRKVST 783
            +F T     +   HP C  V+  +G G  K          +DP + + AIE +L      
Sbjct: 1287 FFNTHQLKCQLQRHPDCANVKQWKG-GPVK----------IDPLALVQAIERYL------ 1346

Query: 784  KKTEQSPEETLREHQIKLLSNIRSCLGVNSELVGSDIMSTDLPEVQVPAEVGADEKSQSS 843
                                 +R    V  +   SD             + G+DE+   S
Sbjct: 1347 --------------------VVRGYGRVREDDEDSD-------------DDGSDEEIDES 1406

Query: 844  PSCS--KKGTAPRLLLYLEGKQLEP-TMTIYQAILQQHIK----------ENETISGTKV 903
             +      G     L +  G+ L P  MT+YQA+ Q  I+          E+  +    +
Sbjct: 1407 LAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGI 1466

Query: 904  WSHVYTIMYRSAGEVEDNTCNQLFCASDK------APTLQ-----------FSSFFCDIL 963
            W+  +TI Y+   E E++  +   C   K      APT             ++   C  +
Sbjct: 1467 WTKTHTIWYKPVREDEESNKD---CVGGKRGRAQTAPTKTSPRNAKKHDELWNDGVCPSV 1526

Query: 964  DCVL--------PSDLAKGSPAYDVLFLLRSIEGMNRMAFHIMSHERIRAFAEGKIDTLD 1023
               L        P ++    P+ DV+ LLR +  ++R  +++  +   +           
Sbjct: 1527 SNPLEVYLIPTAPENITFEDPSLDVILLLRVLHAVSRYWYYLYDNAMCKEI--------- 1586

Query: 1024 NIKLSVPTVSQNEFVNSKLTEKLEQQMRDSSAVSVGGMPLWCKELMDSCPFLFSFEARRK 1083
                 +PT   +EF+NSKLT K  +Q++D   +  G +P W  EL  +CPF F F+ R+ 
Sbjct: 1587 -----IPT---SEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQM 1646

Query: 1084 YFRIVVFGMP---QYQLHVRSHSDLGTSNDGRSSSGGLPRKKVLVHRDQILDSASKMMDQ 1143
             F +  F      Q  L      +   S D R +   L RKK  V+R+++L  A  +M  
Sbjct: 1647 LFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAP-RLDRKKRTVNREELLKQAESVMQD 1706

Query: 1144 YAHQKVLLEVEYDEEVGTGLGPTLEFYTLVSREFQKYGLGMWRGDHDAFISGKRLNIEGR 1203
                + +LE++Y+ EVGTGLGPTLEFY LVS+E Q+  LG+WRG+ +  +S  + + EG 
Sbjct: 1707 LGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGE-EVTLSNPKGSQEGT 1766

Query: 1204 ETTESPFGLFPRPWPSTLDTDKLHLPEVMKKFVLLGQIVAKAIQDGRVLDIYFSKSFYKL 1263
            +  ++  GLF  P+  T      H+ +V  KF  LG+++AKAI D R++D+     FYK 
Sbjct: 1767 KYIQNLQGLFALPFGRT--AKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKW 1826

Query: 1264 ILGQELSI--YDIQSFDPELGTVLLEFQSLVNRNKILGTVYEENSSSKLEFSY------- 1283
            +L QE S+  +D+   DP +   +   + +V + K L    ++ S +K    Y       
Sbjct: 1827 MLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRL---EQDKSQTKESLQYALETLTM 1886

BLAST of IVF0019533 vs. ExPASy TrEMBL
Match: A0A1S3B665 (E3 ubiquitin-protein ligase UPL4 OS=Cucumis melo OX=3656 GN=LOC103486449 PE=4 SV=1)

HSP 1 Score: 2538.1 bits (6577), Expect = 0.0e+00
Identity = 1282/1282 (100.00%), Postives = 1282/1282 (100.00%), Query Frame = 0

Query: 1    MAVLTFIDFFPTIIQRTALRIVVNVCKKLPSECPPNLIEAVPILCNLLQYDDEELVENVA 60
            MAVLTFIDFFPTIIQRTALRIVVNVCKKLPSECPPNLIEAVPILCNLLQYDDEELVENVA
Sbjct: 234  MAVLTFIDFFPTIIQRTALRIVVNVCKKLPSECPPNLIEAVPILCNLLQYDDEELVENVA 293

Query: 61   RCMIKIAECVHQSSELLDGLCQHGLIQHAIRLINLNSRTTLSQTIYNDLLGVLIKLASGS 120
            RCMIKIAECVHQSSELLDGLCQHGLIQHAIRLINLNSRTTLSQTIYNDLLGVLIKLASGS
Sbjct: 294  RCMIKIAECVHQSSELLDGLCQHGLIQHAIRLINLNSRTTLSQTIYNDLLGVLIKLASGS 353

Query: 121  IVAFETLYELNISNTLKDILSAYNLSHGVSSSCAVVDGQRNQVCEVLKLLNELLPTEDAK 180
            IVAFETLYELNISNTLKDILSAYNLSHGVSSSCAVVDGQRNQVCEVLKLLNELLPTEDAK
Sbjct: 354  IVAFETLYELNISNTLKDILSAYNLSHGVSSSCAVVDGQRNQVCEVLKLLNELLPTEDAK 413

Query: 181  TEQLSEKVSFLVGNPKQLQKFGLDILPLLVQVVSSGANLYVCCGCLTIIYKFVCLGESDM 240
            TEQLSEKVSFLVGNPKQLQKFGLDILPLLVQVVSSGANLYVCCGCLTIIYKFVCLGESDM
Sbjct: 414  TEQLSEKVSFLVGNPKQLQKFGLDILPLLVQVVSSGANLYVCCGCLTIIYKFVCLGESDM 473

Query: 241  LVELLENSNISSFLAGVFTRKDHHVLMLGLKITEIILQKLASIFLKSFVKEGVYFAIDAL 300
            LVELLENSNISSFLAGVFTRKDHHVLMLGLKITEIILQKLASIFLKSFVKEGVYFAIDAL
Sbjct: 474  LVELLENSNISSFLAGVFTRKDHHVLMLGLKITEIILQKLASIFLKSFVKEGVYFAIDAL 533

Query: 301  ISPEKYKQLIFPVFTGVHSSFDSCQKSSREHGKCLCYAFSSSCFPSGSETGSCKLDKDSV 360
            ISPEKYKQLIFPVFTGVHSSFDSCQKSSREHGKCLCYAFSSSCFPSGSETGSCKLDKDSV
Sbjct: 534  ISPEKYKQLIFPVFTGVHSSFDSCQKSSREHGKCLCYAFSSSCFPSGSETGSCKLDKDSV 593

Query: 361  YSLANHIRNIYFAEDLCDTDEGVTDILQNLRTFSGALDDLLNLSLIKDTPAQDEEKLYAL 420
            YSLANHIRNIYFAEDLCDTDEGVTDILQNLRTFSGALDDLLNLSLIKDTPAQDEEKLYAL
Sbjct: 594  YSLANHIRNIYFAEDLCDTDEGVTDILQNLRTFSGALDDLLNLSLIKDTPAQDEEKLYAL 653

Query: 421  LAEIMSKLKCGEPISTFEFIESGIVKSFINYITNGQYLRKKEEAQTISRQFSIIERRFEA 480
            LAEIMSKLKCGEPISTFEFIESGIVKSFINYITNGQYLRKKEEAQTISRQFSIIERRFEA
Sbjct: 654  LAEIMSKLKCGEPISTFEFIESGIVKSFINYITNGQYLRKKEEAQTISRQFSIIERRFEA 713

Query: 481  FARLLLSSSDHPSVNLPVLALIRKLQISLSSLENFPVISSQGFKHRNYFATVPNARCVPH 540
            FARLLLSSSDHPSVNLPVLALIRKLQISLSSLENFPVISSQGFKHRNYFATVPNARCVPH
Sbjct: 714  FARLLLSSSDHPSVNLPVLALIRKLQISLSSLENFPVISSQGFKHRNYFATVPNARCVPH 773

Query: 541  PCVKVRFVRGDGETKLCDITGDILTVDPFSSLAAIEGFLWRKVSTKKTEQSPEETLREHQ 600
            PCVKVRFVRGDGETKLCDITGDILTVDPFSSLAAIEGFLWRKVSTKKTEQSPEETLREHQ
Sbjct: 774  PCVKVRFVRGDGETKLCDITGDILTVDPFSSLAAIEGFLWRKVSTKKTEQSPEETLREHQ 833

Query: 601  IKLLSNIRSCLGVNSELVGSDIMSTDLPEVQVPAEVGADEKSQSSPSCSKKGTAPRLLLY 660
            IKLLSNIRSCLGVNSELVGSDIMSTDLPEVQVPAEVGADEKSQSSPSCSKKGTAPRLLLY
Sbjct: 834  IKLLSNIRSCLGVNSELVGSDIMSTDLPEVQVPAEVGADEKSQSSPSCSKKGTAPRLLLY 893

Query: 661  LEGKQLEPTMTIYQAILQQHIKENETISGTKVWSHVYTIMYRSAGEVEDNTCNQLFCASD 720
            LEGKQLEPTMTIYQAILQQHIKENETISGTKVWSHVYTIMYRSAGEVEDNTCNQLFCASD
Sbjct: 894  LEGKQLEPTMTIYQAILQQHIKENETISGTKVWSHVYTIMYRSAGEVEDNTCNQLFCASD 953

Query: 721  KAPTLQFSSFFCDILDCVLPSDLAKGSPAYDVLFLLRSIEGMNRMAFHIMSHERIRAFAE 780
            KAPTLQFSSFFCDILDCVLPSDLAKGSPAYDVLFLLRSIEGMNRMAFHIMSHERIRAFAE
Sbjct: 954  KAPTLQFSSFFCDILDCVLPSDLAKGSPAYDVLFLLRSIEGMNRMAFHIMSHERIRAFAE 1013

Query: 781  GKIDTLDNIKLSVPTVSQNEFVNSKLTEKLEQQMRDSSAVSVGGMPLWCKELMDSCPFLF 840
            GKIDTLDNIKLSVPTVSQNEFVNSKLTEKLEQQMRDSSAVSVGGMPLWCKELMDSCPFLF
Sbjct: 1014 GKIDTLDNIKLSVPTVSQNEFVNSKLTEKLEQQMRDSSAVSVGGMPLWCKELMDSCPFLF 1073

Query: 841  SFEARRKYFRIVVFGMPQYQLHVRSHSDLGTSNDGRSSSGGLPRKKVLVHRDQILDSASK 900
            SFEARRKYFRIVVFGMPQYQLHVRSHSDLGTSNDGRSSSGGLPRKKVLVHRDQILDSASK
Sbjct: 1074 SFEARRKYFRIVVFGMPQYQLHVRSHSDLGTSNDGRSSSGGLPRKKVLVHRDQILDSASK 1133

Query: 901  MMDQYAHQKVLLEVEYDEEVGTGLGPTLEFYTLVSREFQKYGLGMWRGDHDAFISGKRLN 960
            MMDQYAHQKVLLEVEYDEEVGTGLGPTLEFYTLVSREFQKYGLGMWRGDHDAFISGKRLN
Sbjct: 1134 MMDQYAHQKVLLEVEYDEEVGTGLGPTLEFYTLVSREFQKYGLGMWRGDHDAFISGKRLN 1193

Query: 961  IEGRETTESPFGLFPRPWPSTLDTDKLHLPEVMKKFVLLGQIVAKAIQDGRVLDIYFSKS 1020
            IEGRETTESPFGLFPRPWPSTLDTDKLHLPEVMKKFVLLGQIVAKAIQDGRVLDIYFSKS
Sbjct: 1194 IEGRETTESPFGLFPRPWPSTLDTDKLHLPEVMKKFVLLGQIVAKAIQDGRVLDIYFSKS 1253

Query: 1021 FYKLILGQELSIYDIQSFDPELGTVLLEFQSLVNRNKILGTVYEENSSSKLEFSYHNTNI 1080
            FYKLILGQELSIYDIQSFDPELGTVLLEFQSLVNRNKILGTVYEENSSSKLEFSYHNTNI
Sbjct: 1254 FYKLILGQELSIYDIQSFDPELGTVLLEFQSLVNRNKILGTVYEENSSSKLEFSYHNTNI 1313

Query: 1081 EDLCLDFTLPGYPDYLLTSSQDNSMVNAKNLEDYVSLVADATLYSGISRQIEAFKSGFNQ 1140
            EDLCLDFTLPGYPDYLLTSSQDNSMVNAKNLEDYVSLVADATLYSGISRQIEAFKSGFNQ
Sbjct: 1314 EDLCLDFTLPGYPDYLLTSSQDNSMVNAKNLEDYVSLVADATLYSGISRQIEAFKSGFNQ 1373

Query: 1141 VFPIEHLQVFTAEELERLICGEQDIWALSDLLDNMKFDHGYTSSSPSIIHLLEIIQEFDN 1200
            VFPIEHLQVFTAEELERLICGEQDIWALSDLLDNMKFDHGYTSSSPSIIHLLEIIQEFDN
Sbjct: 1374 VFPIEHLQVFTAEELERLICGEQDIWALSDLLDNMKFDHGYTSSSPSIIHLLEIIQEFDN 1433

Query: 1201 KQQRAFLQFVTGAPRLPSGGFASLNPKLTIVRKHSSNLVDYDLPSVMTCANYLKLPPYSS 1260
            KQQRAFLQFVTGAPRLPSGGFASLNPKLTIVRKHSSNLVDYDLPSVMTCANYLKLPPYSS
Sbjct: 1434 KQQRAFLQFVTGAPRLPSGGFASLNPKLTIVRKHSSNLVDYDLPSVMTCANYLKLPPYSS 1493

Query: 1261 KEIMKEKLLYAITEGQGSFHLS 1283
            KEIMKEKLLYAITEGQGSFHLS
Sbjct: 1494 KEIMKEKLLYAITEGQGSFHLS 1515

BLAST of IVF0019533 vs. ExPASy TrEMBL
Match: A0A5D3DN91 (E3 ubiquitin-protein ligase UPL4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G002270 PE=4 SV=1)

HSP 1 Score: 2533.4 bits (6565), Expect = 0.0e+00
Identity = 1282/1283 (99.92%), Postives = 1282/1283 (99.92%), Query Frame = 0

Query: 1    MAVLTFIDFFPTIIQRTALRIVVNVCKKLPSECPPNLIEAVPILCNLLQYDDEELVENVA 60
            MAVLTFIDFFPTIIQRTALRIVVNVCKKLPSECPPNLIEAVPILCNLLQYDDEELVENVA
Sbjct: 234  MAVLTFIDFFPTIIQRTALRIVVNVCKKLPSECPPNLIEAVPILCNLLQYDDEELVENVA 293

Query: 61   RCMIKIAECVHQSSELLDGLCQHGLIQHAIRLINLNSRTTLSQTIYNDLLGVLIKLASGS 120
            RCMIKIAECVHQSSELLDGLCQHGLIQHAIRLINLNSRTTLSQTIYNDLLGVLIKLASGS
Sbjct: 294  RCMIKIAECVHQSSELLDGLCQHGLIQHAIRLINLNSRTTLSQTIYNDLLGVLIKLASGS 353

Query: 121  IVAFETLYELNISNTLKDILSAYNLSHGVSSSCAVVDGQRNQVCEVLKLLNELLPTEDAK 180
            IVAFETLYELNISNTLKDILSAYNLSHGVSSSCAVVDGQRNQVCEVLKLLNELLPTEDAK
Sbjct: 354  IVAFETLYELNISNTLKDILSAYNLSHGVSSSCAVVDGQRNQVCEVLKLLNELLPTEDAK 413

Query: 181  TEQLSEKVSFLVGNPKQLQKFGLDILPLLVQ-VVSSGANLYVCCGCLTIIYKFVCLGESD 240
            TEQLSEKVSFLVGNPKQLQKFGLDILPLLVQ VVSSGANLYVCCGCLTIIYKFVCLGESD
Sbjct: 414  TEQLSEKVSFLVGNPKQLQKFGLDILPLLVQVVVSSGANLYVCCGCLTIIYKFVCLGESD 473

Query: 241  MLVELLENSNISSFLAGVFTRKDHHVLMLGLKITEIILQKLASIFLKSFVKEGVYFAIDA 300
            MLVELLENSNISSFLAGVFTRKDHHVLMLGLKITEIILQKLASIFLKSFVKEGVYFAIDA
Sbjct: 474  MLVELLENSNISSFLAGVFTRKDHHVLMLGLKITEIILQKLASIFLKSFVKEGVYFAIDA 533

Query: 301  LISPEKYKQLIFPVFTGVHSSFDSCQKSSREHGKCLCYAFSSSCFPSGSETGSCKLDKDS 360
            LISPEKYKQLIFPVFTGVHSSFDSCQKSSREHGKCLCYAFSSSCFPSGSETGSCKLDKDS
Sbjct: 534  LISPEKYKQLIFPVFTGVHSSFDSCQKSSREHGKCLCYAFSSSCFPSGSETGSCKLDKDS 593

Query: 361  VYSLANHIRNIYFAEDLCDTDEGVTDILQNLRTFSGALDDLLNLSLIKDTPAQDEEKLYA 420
            VYSLANHIRNIYFAEDLCDTDEGVTDILQNLRTFSGALDDLLNLSLIKDTPAQDEEKLYA
Sbjct: 594  VYSLANHIRNIYFAEDLCDTDEGVTDILQNLRTFSGALDDLLNLSLIKDTPAQDEEKLYA 653

Query: 421  LLAEIMSKLKCGEPISTFEFIESGIVKSFINYITNGQYLRKKEEAQTISRQFSIIERRFE 480
            LLAEIMSKLKCGEPISTFEFIESGIVKSFINYITNGQYLRKKEEAQTISRQFSIIERRFE
Sbjct: 654  LLAEIMSKLKCGEPISTFEFIESGIVKSFINYITNGQYLRKKEEAQTISRQFSIIERRFE 713

Query: 481  AFARLLLSSSDHPSVNLPVLALIRKLQISLSSLENFPVISSQGFKHRNYFATVPNARCVP 540
            AFARLLLSSSDHPSVNLPVLALIRKLQISLSSLENFPVISSQGFKHRNYFATVPNARCVP
Sbjct: 714  AFARLLLSSSDHPSVNLPVLALIRKLQISLSSLENFPVISSQGFKHRNYFATVPNARCVP 773

Query: 541  HPCVKVRFVRGDGETKLCDITGDILTVDPFSSLAAIEGFLWRKVSTKKTEQSPEETLREH 600
            HPCVKVRFVRGDGETKLCDITGDILTVDPFSSLAAIEGFLWRKVSTKKTEQSPEETLREH
Sbjct: 774  HPCVKVRFVRGDGETKLCDITGDILTVDPFSSLAAIEGFLWRKVSTKKTEQSPEETLREH 833

Query: 601  QIKLLSNIRSCLGVNSELVGSDIMSTDLPEVQVPAEVGADEKSQSSPSCSKKGTAPRLLL 660
            QIKLLSNIRSCLGVNSELVGSDIMSTDLPEVQVPAEVGADEKSQSSPSCSKKGTAPRLLL
Sbjct: 834  QIKLLSNIRSCLGVNSELVGSDIMSTDLPEVQVPAEVGADEKSQSSPSCSKKGTAPRLLL 893

Query: 661  YLEGKQLEPTMTIYQAILQQHIKENETISGTKVWSHVYTIMYRSAGEVEDNTCNQLFCAS 720
            YLEGKQLEPTMTIYQAILQQHIKENETISGTKVWSHVYTIMYRSAGEVEDNTCNQLFCAS
Sbjct: 894  YLEGKQLEPTMTIYQAILQQHIKENETISGTKVWSHVYTIMYRSAGEVEDNTCNQLFCAS 953

Query: 721  DKAPTLQFSSFFCDILDCVLPSDLAKGSPAYDVLFLLRSIEGMNRMAFHIMSHERIRAFA 780
            DKAPTLQFSSFFCDILDCVLPSDLAKGSPAYDVLFLLRSIEGMNRMAFHIMSHERIRAFA
Sbjct: 954  DKAPTLQFSSFFCDILDCVLPSDLAKGSPAYDVLFLLRSIEGMNRMAFHIMSHERIRAFA 1013

Query: 781  EGKIDTLDNIKLSVPTVSQNEFVNSKLTEKLEQQMRDSSAVSVGGMPLWCKELMDSCPFL 840
            EGKIDTLDNIKLSVPTVSQNEFVNSKLTEKLEQQMRDSSAVSVGGMPLWCKELMDSCPFL
Sbjct: 1014 EGKIDTLDNIKLSVPTVSQNEFVNSKLTEKLEQQMRDSSAVSVGGMPLWCKELMDSCPFL 1073

Query: 841  FSFEARRKYFRIVVFGMPQYQLHVRSHSDLGTSNDGRSSSGGLPRKKVLVHRDQILDSAS 900
            FSFEARRKYFRIVVFGMPQYQLHVRSHSDLGTSNDGRSSSGGLPRKKVLVHRDQILDSAS
Sbjct: 1074 FSFEARRKYFRIVVFGMPQYQLHVRSHSDLGTSNDGRSSSGGLPRKKVLVHRDQILDSAS 1133

Query: 901  KMMDQYAHQKVLLEVEYDEEVGTGLGPTLEFYTLVSREFQKYGLGMWRGDHDAFISGKRL 960
            KMMDQYAHQKVLLEVEYDEEVGTGLGPTLEFYTLVSREFQKYGLGMWRGDHDAFISGKRL
Sbjct: 1134 KMMDQYAHQKVLLEVEYDEEVGTGLGPTLEFYTLVSREFQKYGLGMWRGDHDAFISGKRL 1193

Query: 961  NIEGRETTESPFGLFPRPWPSTLDTDKLHLPEVMKKFVLLGQIVAKAIQDGRVLDIYFSK 1020
            NIEGRETTESPFGLFPRPWPSTLDTDKLHLPEVMKKFVLLGQIVAKAIQDGRVLDIYFSK
Sbjct: 1194 NIEGRETTESPFGLFPRPWPSTLDTDKLHLPEVMKKFVLLGQIVAKAIQDGRVLDIYFSK 1253

Query: 1021 SFYKLILGQELSIYDIQSFDPELGTVLLEFQSLVNRNKILGTVYEENSSSKLEFSYHNTN 1080
            SFYKLILGQELSIYDIQSFDPELGTVLLEFQSLVNRNKILGTVYEENSSSKLEFSYHNTN
Sbjct: 1254 SFYKLILGQELSIYDIQSFDPELGTVLLEFQSLVNRNKILGTVYEENSSSKLEFSYHNTN 1313

Query: 1081 IEDLCLDFTLPGYPDYLLTSSQDNSMVNAKNLEDYVSLVADATLYSGISRQIEAFKSGFN 1140
            IEDLCLDFTLPGYPDYLLTSSQDNSMVNAKNLEDYVSLVADATLYSGISRQIEAFKSGFN
Sbjct: 1314 IEDLCLDFTLPGYPDYLLTSSQDNSMVNAKNLEDYVSLVADATLYSGISRQIEAFKSGFN 1373

Query: 1141 QVFPIEHLQVFTAEELERLICGEQDIWALSDLLDNMKFDHGYTSSSPSIIHLLEIIQEFD 1200
            QVFPIEHLQVFTAEELERLICGEQDIWALSDLLDNMKFDHGYTSSSPSIIHLLEIIQEFD
Sbjct: 1374 QVFPIEHLQVFTAEELERLICGEQDIWALSDLLDNMKFDHGYTSSSPSIIHLLEIIQEFD 1433

Query: 1201 NKQQRAFLQFVTGAPRLPSGGFASLNPKLTIVRKHSSNLVDYDLPSVMTCANYLKLPPYS 1260
            NKQQRAFLQFVTGAPRLPSGGFASLNPKLTIVRKHSSNLVDYDLPSVMTCANYLKLPPYS
Sbjct: 1434 NKQQRAFLQFVTGAPRLPSGGFASLNPKLTIVRKHSSNLVDYDLPSVMTCANYLKLPPYS 1493

Query: 1261 SKEIMKEKLLYAITEGQGSFHLS 1283
            SKEIMKEKLLYAITEGQGSFHLS
Sbjct: 1494 SKEIMKEKLLYAITEGQGSFHLS 1516

BLAST of IVF0019533 vs. ExPASy TrEMBL
Match: A0A5A7TQD3 (E3 ubiquitin-protein ligase UPL4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold236G003840 PE=4 SV=1)

HSP 1 Score: 2523.8 bits (6540), Expect = 0.0e+00
Identity = 1282/1308 (98.01%), Postives = 1282/1308 (98.01%), Query Frame = 0

Query: 1    MAVLTFIDFFPTIIQRTALRIVVNVCKKLPSECPPNLIEAVPILCNLLQYDDEELVENVA 60
            MAVLTFIDFFPTIIQRTALRIVVNVCKKLPSECPPNLIEAVPILCNLLQYDDEELVENVA
Sbjct: 224  MAVLTFIDFFPTIIQRTALRIVVNVCKKLPSECPPNLIEAVPILCNLLQYDDEELVENVA 283

Query: 61   RCMIKIAECVHQSSELLDGLCQHGLIQHAIRLINLNSRTTLSQTIYNDLLGVLIKLASGS 120
            RCMIKIAECVHQSSELLDGLCQHGLIQHAIRLINLNSRTTLSQTIYNDLLGVLIKLASGS
Sbjct: 284  RCMIKIAECVHQSSELLDGLCQHGLIQHAIRLINLNSRTTLSQTIYNDLLGVLIKLASGS 343

Query: 121  IVAFETLYELNISNTLKDILSAYNLSHGVSSSCAVVDGQRNQVCEVLKLLNELLPTEDAK 180
            IVAFETLYELNISNTLKDILSAYNLSHGVSSSCAVVDGQRNQVCEVLKLLNELLPTEDAK
Sbjct: 344  IVAFETLYELNISNTLKDILSAYNLSHGVSSSCAVVDGQRNQVCEVLKLLNELLPTEDAK 403

Query: 181  TEQLSEKVSFLVGNPKQLQKFGLDILPLLVQVVSSGANLYVCCGCLTIIYKFVCLGESDM 240
            TEQLSEKVSFLVGNPKQLQKFGLDILPLLVQVVSSGANLYVCCGCLTIIYKFVCLGESDM
Sbjct: 404  TEQLSEKVSFLVGNPKQLQKFGLDILPLLVQVVSSGANLYVCCGCLTIIYKFVCLGESDM 463

Query: 241  LVELLENSNISSFLAGVFTRKDHHVLMLGLKITEIILQKLASIFLKSFVKEGVYFAIDAL 300
            LVELLENSNISSFLAGVFTRKDHHVLMLGLKITEIILQKLASIFLKSFVKEGVYFAIDAL
Sbjct: 464  LVELLENSNISSFLAGVFTRKDHHVLMLGLKITEIILQKLASIFLKSFVKEGVYFAIDAL 523

Query: 301  ISPEKYKQLIFPVFTGVHSSFDSCQKSSREHGKCLCYAFSSSCFPSGSETGSCKLDKDSV 360
            ISPEKYKQLIFPVFTGVHSSFDSCQKSSREHGKCLCYAFSSSCFPSGSETGSCKLDKDSV
Sbjct: 524  ISPEKYKQLIFPVFTGVHSSFDSCQKSSREHGKCLCYAFSSSCFPSGSETGSCKLDKDSV 583

Query: 361  YSLANHIRNIYFAEDLCDTDEGVTDILQNLRTFSGALDDLLNLSLIKDTPAQDEEKLYAL 420
            YSLANHIRNIYFAEDLCDTDEGVTDILQNLRTFSGALDDLLNLSLIKDTPAQDEEKLYAL
Sbjct: 584  YSLANHIRNIYFAEDLCDTDEGVTDILQNLRTFSGALDDLLNLSLIKDTPAQDEEKLYAL 643

Query: 421  LAEIMSKLKCGEPISTFEFIESGIVKSFINYITNGQYLRKKEEAQTISRQFSIIERRFEA 480
            LAEIMSKLKCGEPISTFEFIESGIVKSFINYITNGQYLRKKEEAQTISRQFSIIERRFEA
Sbjct: 644  LAEIMSKLKCGEPISTFEFIESGIVKSFINYITNGQYLRKKEEAQTISRQFSIIERRFEA 703

Query: 481  FARLLLSSSDHPSVNLPVLALIRKLQISLSSLENFPVISSQGFKHRNYFATVPNARCVPH 540
            FARLLLSSSDHPSVNLPVLALIRKLQISLSSLENFPVISSQGFKHRNYFATVPNARCVPH
Sbjct: 704  FARLLLSSSDHPSVNLPVLALIRKLQISLSSLENFPVISSQGFKHRNYFATVPNARCVPH 763

Query: 541  PCVKVRFVRGDGETKLCDITGDILTVDPFSSLAAIEGFLWRKVSTKKTEQSPEETLREHQ 600
            PCVKVRFVRGDGETKLCDITGDILTVDPFSSLAAIEGFLWRKVSTKKTEQSPEETLREHQ
Sbjct: 764  PCVKVRFVRGDGETKLCDITGDILTVDPFSSLAAIEGFLWRKVSTKKTEQSPEETLREHQ 823

Query: 601  IKLLSNIRSCLGVNSELVGSDIMSTDLPEVQVPAEVGADEKSQSSPSCSKKGTAPRLLLY 660
            IKLLSNIRSCLGVNSELVGSDIMSTDLPEVQVPAEVGADEKSQSSPSCSKKGTAPRLLLY
Sbjct: 824  IKLLSNIRSCLGVNSELVGSDIMSTDLPEVQVPAEVGADEKSQSSPSCSKKGTAPRLLLY 883

Query: 661  LEGKQLEPTMTIYQAILQQHIKENETISGTKVWSHVYTIMYRSAGEVEDNTCNQLFCASD 720
            LEGKQLEPTMTIYQAILQQHIKENETISGTKVWSHVYTIMYRSAGEVEDNTCNQLFCASD
Sbjct: 884  LEGKQLEPTMTIYQAILQQHIKENETISGTKVWSHVYTIMYRSAGEVEDNTCNQLFCASD 943

Query: 721  KAPTLQFSSFFCDILDCVLPSDLAKGSPAYDVLFLLRSIEGMNRMAFHIMSHERIRAFAE 780
            KAPTLQFSSFFCDILDCVLPSDLAKGSPAYDVLFLLRSIEGMNRMAFHIMSHERIRAFAE
Sbjct: 944  KAPTLQFSSFFCDILDCVLPSDLAKGSPAYDVLFLLRSIEGMNRMAFHIMSHERIRAFAE 1003

Query: 781  GKIDTLDNIKLSVPTVSQNEFVNSKLTEKLEQQMRDSSAVSVGGMPLWCKELMDSCPFLF 840
            GKIDTLDNIKLSVPTVSQNEFVNSKLTEKLEQQMRDSSAVSVGGMPLWCKELMDSCPFLF
Sbjct: 1004 GKIDTLDNIKLSVPTVSQNEFVNSKLTEKLEQQMRDSSAVSVGGMPLWCKELMDSCPFLF 1063

Query: 841  SFEARRKYFRIVVFGMPQYQLHVRSHSDLGTSNDGRSSSGGLPRKKVLVHRDQILDSASK 900
            SFEARRKYFRIVVFGMPQYQLHVRSHSDLGTSNDGRSSSGGLPRKKVLVHRDQILDSASK
Sbjct: 1064 SFEARRKYFRIVVFGMPQYQLHVRSHSDLGTSNDGRSSSGGLPRKKVLVHRDQILDSASK 1123

Query: 901  MMDQYAHQKVLLEVEYDEEVGTGLGPTLEFYTLVSREFQKYGLGMWRGDHDAFISGKRLN 960
            MMDQYAHQKVLLEVEYDEEVGTGLGPTLEFYTLVSREFQKYGLGMWRGDHDAFISGKRLN
Sbjct: 1124 MMDQYAHQKVLLEVEYDEEVGTGLGPTLEFYTLVSREFQKYGLGMWRGDHDAFISGKRLN 1183

Query: 961  IEGRETTESPFGLFPRPWPSTLDTDKLHLPEVMKKFVLLGQIVAKAIQDGRVLDIYFSKS 1020
            IEGRETTESPFGLFPRPWPSTLDTDKLHLPEVMKKFVLLGQIVAKAIQDGRVLDIYFSKS
Sbjct: 1184 IEGRETTESPFGLFPRPWPSTLDTDKLHLPEVMKKFVLLGQIVAKAIQDGRVLDIYFSKS 1243

Query: 1021 FYKLILGQELSIYDIQSFDPELGTVLLEFQSLVNRNKILGTVYEENSSSKLEFSYHNTNI 1080
            FYKLILGQELSIYDIQSFDPELGTVLLEFQSLVNRNKILGTVYEENSSSKLEFSYHNTNI
Sbjct: 1244 FYKLILGQELSIYDIQSFDPELGTVLLEFQSLVNRNKILGTVYEENSSSKLEFSYHNTNI 1303

Query: 1081 EDLCLDFTLPGYPDYLLTSSQDNSMVNAKNLEDYVSLVADATLYSGISRQIEAFKSGFNQ 1140
            EDLCLDFTLPGYPDYLLTSSQDNSMVNAKNLEDYVSLVADATLYSGISRQIEAFKSGFNQ
Sbjct: 1304 EDLCLDFTLPGYPDYLLTSSQDNSMVNAKNLEDYVSLVADATLYSGISRQIEAFKSGFNQ 1363

Query: 1141 VFPIEHLQVFTAEELERLICGEQDIWA--------------------------LSDLLDN 1200
            VFPIEHLQVFTAEELERLICGEQDIWA                          LSDLLDN
Sbjct: 1364 VFPIEHLQVFTAEELERLICGEQDIWAVRISANYHIYLKLLTISLVLFNLLMQLSDLLDN 1423

Query: 1201 MKFDHGYTSSSPSIIHLLEIIQEFDNKQQRAFLQFVTGAPRLPSGGFASLNPKLTIVRKH 1260
            MKFDHGYTSSSPSIIHLLEIIQEFDNKQQRAFLQFVTGAPRLPSGGFASLNPKLTIVRKH
Sbjct: 1424 MKFDHGYTSSSPSIIHLLEIIQEFDNKQQRAFLQFVTGAPRLPSGGFASLNPKLTIVRKH 1483

Query: 1261 SSNLVDYDLPSVMTCANYLKLPPYSSKEIMKEKLLYAITEGQGSFHLS 1283
            SSNLVDYDLPSVMTCANYLKLPPYSSKEIMKEKLLYAITEGQGSFHLS
Sbjct: 1484 SSNLVDYDLPSVMTCANYLKLPPYSSKEIMKEKLLYAITEGQGSFHLS 1531

BLAST of IVF0019533 vs. ExPASy TrEMBL
Match: A0A0A0LDW7 (HECT domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G736800 PE=4 SV=1)

HSP 1 Score: 2396.7 bits (6210), Expect = 0.0e+00
Identity = 1218/1283 (94.93%), Postives = 1242/1283 (96.80%), Query Frame = 0

Query: 1    MAVLTFIDFFPTIIQRTALRIVVNVCKKLPSECPPNLIEAVPILCNLLQYDDEELVENVA 60
            MAVLTFIDFFPTIIQRTALRIVVNVCKKLPSECP NLIEAVPILCNLLQYDDEELVENVA
Sbjct: 234  MAVLTFIDFFPTIIQRTALRIVVNVCKKLPSECPQNLIEAVPILCNLLQYDDEELVENVA 293

Query: 61   RCMIKIAECVHQSSELLDGLCQHGLIQHAIRLINLNSRTTLSQTIYNDLLGVLIKLASGS 120
            RCMIKIAECVHQSSELLDGLCQHGLIQHAIRLINLNSRTTLSQTIYNDLLGVLIKLASGS
Sbjct: 294  RCMIKIAECVHQSSELLDGLCQHGLIQHAIRLINLNSRTTLSQTIYNDLLGVLIKLASGS 353

Query: 121  IVAFETLYELNISNTLKDILSAYNLSHGVSSSCAVVDGQRNQVCEVLKLLNELLPTEDAK 180
            IVAFETLYELNISNTLKDILSAYNLSHGVSSSCAVVDGQRNQVCEVLKLLNELLPTEDAK
Sbjct: 354  IVAFETLYELNISNTLKDILSAYNLSHGVSSSCAVVDGQRNQVCEVLKLLNELLPTEDAK 413

Query: 181  TEQLSEKVSFLVGNPKQLQKFGLDILPLLVQVVSSGANLYVCCGCLTIIYKFVCLGESDM 240
            TEQLSEKVSFLV NPKQLQKFGLDILPLLVQVVSSGANLYVCCGCLTIIYKFVCLGESDM
Sbjct: 414  TEQLSEKVSFLVSNPKQLQKFGLDILPLLVQVVSSGANLYVCCGCLTIIYKFVCLGESDM 473

Query: 241  LVELLENSNISSFLAGVFTRKDHHVLMLGLKITEIILQKLASIFLKSFVKEGVYFAIDAL 300
            LVELLENSNISSFLAGVFTRKDHHVLMLGLKITEIILQKLAS FLKSFVKEGVYF+IDAL
Sbjct: 474  LVELLENSNISSFLAGVFTRKDHHVLMLGLKITEIILQKLASTFLKSFVKEGVYFSIDAL 533

Query: 301  ISPEKYKQLIFPVFTGVHSSFDSCQKSSREHGKCLCYAFSSSCFPSGSETGSCKLDKDSV 360
            ISP+KYKQLIFPVFTGVHSSF SCQKSSREHG+CLCYAFSSSCFPS SETGSCKLDKDSV
Sbjct: 534  ISPDKYKQLIFPVFTGVHSSFGSCQKSSREHGRCLCYAFSSSCFPSVSETGSCKLDKDSV 593

Query: 361  YSLANHIRNIYFAEDLCDTDEGVTDILQNLRTFSGALDDLLNLSLIKDTPAQDEEKLYAL 420
            YSLANHIR+IYFAEDLCDTDEGVTDILQNLRTFSGALDDLLNLSLIKDTPAQDEEKLYAL
Sbjct: 594  YSLANHIRSIYFAEDLCDTDEGVTDILQNLRTFSGALDDLLNLSLIKDTPAQDEEKLYAL 653

Query: 421  LAEIMSKLKCGEPISTFEFIESGIVKSFINYITNGQYLRKKEEAQTISRQFSIIERRFEA 480
            LAEIMSKLKCGEPISTFEFIESGIVKSFINYITNGQYLRKK E+Q ISR FSIIERRFEA
Sbjct: 654  LAEIMSKLKCGEPISTFEFIESGIVKSFINYITNGQYLRKKGESQPISRHFSIIERRFEA 713

Query: 481  FARLLLSSSDHPSVNLPVLALIRKLQISLSSLENFPV-ISSQGFKHRNYFATVPNARCVP 540
            FARLLLSSSDHPSVNLPVLALIRKLQISLSSLENF V ISSQGFKHRNYF TVPNARCVP
Sbjct: 714  FARLLLSSSDHPSVNLPVLALIRKLQISLSSLENFHVIISSQGFKHRNYFVTVPNARCVP 773

Query: 541  HPCVKVRFVRGDGETKLCDITGDILTVDPFSSLAAIEGFLWRKVSTKKTEQSPEETLREH 600
            HPCVKVRFVRGDGET LCDI GDIL VDPFSSL AIEGFLW KVS++KTEQSPE+TLREH
Sbjct: 774  HPCVKVRFVRGDGETDLCDINGDILNVDPFSSLTAIEGFLWPKVSSQKTEQSPEDTLREH 833

Query: 601  QIKLLSNIRSCLGVNSELVGSDIMSTDLPEVQVPAEVGADEKSQSSPSCSKKGTAPRLLL 660
            QIKLL          S+LVGSDIMSTDLPEVQVPAEV ADEKSQ S SCSKKGTAPRLLL
Sbjct: 834  QIKLL----------SKLVGSDIMSTDLPEVQVPAEVSADEKSQCSASCSKKGTAPRLLL 893

Query: 661  YLEGKQLEPTMTIYQAILQQHIKENETISGTKVWSHVYTIMYRSAGEVEDNTCNQLFCAS 720
            YLEGKQLEPT++IYQAILQQHIKENETISG K+WS VYTIMYRSAGEVED+TCNQLFCAS
Sbjct: 894  YLEGKQLEPTLSIYQAILQQHIKENETISGIKIWSQVYTIMYRSAGEVEDSTCNQLFCAS 953

Query: 721  DKAPTLQFSSFFCDILDCVLPSDLAKGSPAYDVLFLLRSIEGMNRMAFHIMSHERIRAFA 780
            DKA  LQFSSFFCDILDCVLPSDLAKGSPAYDVLFLLRSIEGMNRMAFHIMSHERIRAFA
Sbjct: 954  DKALKLQFSSFFCDILDCVLPSDLAKGSPAYDVLFLLRSIEGMNRMAFHIMSHERIRAFA 1013

Query: 781  EGKIDTLDNIKLSVPTVSQNEFVNSKLTEKLEQQMRDSSAVSVGGMPLWCKELMDSCPFL 840
            +GKIDTLDNIKLSVP+VSQNEFVNSKLTEKLEQQMRD SAVSVGGMPLWCKELMDSCPFL
Sbjct: 1014 DGKIDTLDNIKLSVPSVSQNEFVNSKLTEKLEQQMRDFSAVSVGGMPLWCKELMDSCPFL 1073

Query: 841  FSFEARRKYFRIVVFGMPQYQLHVRSHSDLGTSNDGRSSSGGLPRKKVLVHRDQILDSAS 900
            FSFEARRKYFRIVVFGMPQYQLH RSHSD GTSNDGRSSSGGLPRKKVLVHR QILDSAS
Sbjct: 1074 FSFEARRKYFRIVVFGMPQYQLHARSHSDFGTSNDGRSSSGGLPRKKVLVHRSQILDSAS 1133

Query: 901  KMMDQYAHQKVLLEVEYDEEVGTGLGPTLEFYTLVSREFQKYGLGMWRGDHDAFISGKRL 960
            KMM+QYA+QKVLLEVEYDEEVGTGLGPTLEFYTLVSREFQK GLGMWRGDHDAFISGKRL
Sbjct: 1134 KMMNQYANQKVLLEVEYDEEVGTGLGPTLEFYTLVSREFQKNGLGMWRGDHDAFISGKRL 1193

Query: 961  NIEGRETTESPFGLFPRPWPSTLDTDKLHLPEVMKKFVLLGQIVAKAIQDGRVLDIYFSK 1020
            NIE RETTESPFGLFPRPWPSTLDTDKLHLPEVMKKFVLLGQIVAKAIQD RVLDIYFSK
Sbjct: 1194 NIEDRETTESPFGLFPRPWPSTLDTDKLHLPEVMKKFVLLGQIVAKAIQDCRVLDIYFSK 1253

Query: 1021 SFYKLILGQELSIYDIQSFDPELGTVLLEFQSLVNRNKILGTVYEENSSSKLEFSYHNTN 1080
            +FYKLILGQELSIYDIQSFDPELGTVLLEFQ+LVNRNK+LG+VYEENSSSKLEFSYHNTN
Sbjct: 1254 AFYKLILGQELSIYDIQSFDPELGTVLLEFQALVNRNKLLGSVYEENSSSKLEFSYHNTN 1313

Query: 1081 IEDLCLDFTLPGYPDYLLTSSQDNSMVNAKNLEDYVSLVADATLYSGISRQIEAFKSGFN 1140
            IEDLCLDFTLPGYPDYLLTSSQDNSMVNAKNLE+YVSLVADATL SGISRQIEAFKSGFN
Sbjct: 1314 IEDLCLDFTLPGYPDYLLTSSQDNSMVNAKNLENYVSLVADATLCSGISRQIEAFKSGFN 1373

Query: 1141 QVFPIEHLQVFTAEELERLICGEQDIWALSDLLDNMKFDHGYTSSSPSIIHLLEIIQEFD 1200
            QVFPIEHLQVFTAEELERLICGEQDIWALSDLLDNMKFDHGYTSSSPSI+HLLEIIQ+FD
Sbjct: 1374 QVFPIEHLQVFTAEELERLICGEQDIWALSDLLDNMKFDHGYTSSSPSIVHLLEIIQDFD 1433

Query: 1201 NKQQRAFLQFVTGAPRLPSGGFASLNPKLTIVRKHSSNLVDYDLPSVMTCANYLKLPPYS 1260
            NKQQRAFLQFVTGAPRLPSGGFASLNPKLTIVRKHSSNLVDYDLPSVMTCANYLKLPPYS
Sbjct: 1434 NKQQRAFLQFVTGAPRLPSGGFASLNPKLTIVRKHSSNLVDYDLPSVMTCANYLKLPPYS 1493

Query: 1261 SKEIMKEKLLYAITEGQGSFHLS 1283
            SKEIMKEKLLYAITEGQGSFHLS
Sbjct: 1494 SKEIMKEKLLYAITEGQGSFHLS 1506

BLAST of IVF0019533 vs. ExPASy TrEMBL
Match: A0A6J1J7K5 (E3 ubiquitin-protein ligase UPL4-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111482087 PE=4 SV=1)

HSP 1 Score: 2197.9 bits (5694), Expect = 0.0e+00
Identity = 1112/1283 (86.67%), Postives = 1183/1283 (92.21%), Query Frame = 0

Query: 1    MAVLTFIDFFPTIIQRTALRIVVNVCKKLPSECPPNLIEAVPILCNLLQYDDEELVENVA 60
            MAVLTFIDFF + IQRTALR VVN+CKKLPSECP  LIEAVPILCNL Q+DD ELVENVA
Sbjct: 234  MAVLTFIDFFSSSIQRTALRTVVNICKKLPSECPLTLIEAVPILCNLFQHDDGELVENVA 293

Query: 61   RCMIKIAECVHQSSELLDGLCQHGLIQHAIRLINLNSRTTLSQTIYNDLLGVLIKLASGS 120
            RCMIKIAEC HQS E+LDGLCQHGLIQ  IRLINLNSRT+LSQTIYNDLLG+L+KL+SGS
Sbjct: 294  RCMIKIAECAHQSCEILDGLCQHGLIQQVIRLINLNSRTSLSQTIYNDLLGILVKLSSGS 353

Query: 121  IVAFETLYELNISNTLKDILSAYNLSHGVSSSCAVVDGQRNQVCEVLKLLNELLPTEDAK 180
            IVAF+TLYEL+ISNTLK+ILS YNLSHG+ SSCAVVDGQRNQVCEVLKLLNELLPTEDAK
Sbjct: 354  IVAFKTLYELDISNTLKEILSVYNLSHGM-SSCAVVDGQRNQVCEVLKLLNELLPTEDAK 413

Query: 181  TEQLSEKVSFLVGNPKQLQKFGLDILPLLVQVVSSGANLYVCCGCLTIIYKFVCLGESDM 240
             EQLSEKVSFL  NPKQLQKFGLDILPLLVQVVSSGA LYVCCGCLTIIYKF+CLGESDM
Sbjct: 414  AEQLSEKVSFLAINPKQLQKFGLDILPLLVQVVSSGAKLYVCCGCLTIIYKFICLGESDM 473

Query: 241  LVELLENSNISSFLAGVFTRKDHHVLMLGLKITEIILQKLASIFLKSFVKEGVYFAIDAL 300
            LVELL+N+NISSFL GVFTRKDHHVLML LKITEIILQKL+SIFLKSFVKEGVYFAIDAL
Sbjct: 474  LVELLQNANISSFLVGVFTRKDHHVLMLALKITEIILQKLSSIFLKSFVKEGVYFAIDAL 533

Query: 301  ISPEKYKQLIFPVFTGVHSSFDSCQKSSREHGKCLCYAFSSSCFPSGSETGSCKLDKDSV 360
            I+PEKYKQLIFPVF GVH SF SCQKS+RE+G+CLCYAFSS CF S +ETGSCKLDKDSV
Sbjct: 534  ITPEKYKQLIFPVFAGVHPSFGSCQKSTRENGRCLCYAFSSGCFSSVAETGSCKLDKDSV 593

Query: 361  YSLANHIRNIYFAEDLCDTDEGVTDILQNLRTFSGALDDLLNLSLIKDTPAQDEEKLYAL 420
            YSLANHIRN YF ++LCDTD+GV+DILQNL TFSGALDDLLNLSLIKDTPAQDEEK Y L
Sbjct: 594  YSLANHIRNNYFTDELCDTDKGVSDILQNLLTFSGALDDLLNLSLIKDTPAQDEEKFYTL 653

Query: 421  LAEIMSKLKCGEPISTFEFIESGIVKSFINYITNGQYLRKKEEAQTISRQFSIIERRFEA 480
            LAEIMSKLKCGEPISTFEFIESGIVKSFINY+TNGQYLRKK E QTISRQFSI+ERRFEA
Sbjct: 654  LAEIMSKLKCGEPISTFEFIESGIVKSFINYLTNGQYLRKKGEPQTISRQFSIMERRFEA 713

Query: 481  FARLLLSSSDHPSVNLPVLALIRKLQISLSSLENFPVISSQGFKHRNYFATVPNARCVPH 540
            FARLL SSSDHPSVNLPV  L+RKLQISLSSLENFPVISSQGFKHRNYFATVPN RC+PH
Sbjct: 714  FARLLFSSSDHPSVNLPVRVLLRKLQISLSSLENFPVISSQGFKHRNYFATVPNGRCIPH 773

Query: 541  PCVKVRFVRGDGETKLCDITGDILTVDPFSSLAAIEGFLWRKVSTKKTEQSPE-ETLREH 600
            PCVKVRFVRGDGET LCDITGDILTVDPFSSL AIEGFLW KV+ KK EQS E ++LREH
Sbjct: 774  PCVKVRFVRGDGETDLCDITGDILTVDPFSSLNAIEGFLWPKVTRKKAEQSSEADSLREH 833

Query: 601  QIKLLSNIRSCLGVNSELVGSDIMSTDLPEVQVPAEVGADEKSQSSPSCSKKGTAPRLLL 660
            Q+K LSN+ S  GVN ELVGS+  S+DLPE++V  EV  DEKSQ S S SKKGT P+LLL
Sbjct: 834  QVKPLSNVCSYFGVNPELVGSNSKSSDLPEIEVSVEVSTDEKSQGSASSSKKGTKPKLLL 893

Query: 661  YLEGKQLEPTMTIYQAILQQHIKENETISGTKVWSHVYTIMYRSAGEVEDNTCNQLFCAS 720
            YLEGKQLEPT+T+YQ ILQQHIKENE ISGTKVWS VYTIMY+SAGEVEDN+CNQ F AS
Sbjct: 894  YLEGKQLEPTLTLYQTILQQHIKENEAISGTKVWSQVYTIMYKSAGEVEDNSCNQFFSAS 953

Query: 721  DKAPTLQFSSFFCDILDCVLPSDLAKGSPAYDVLFLLRSIEGMNRMAFHIMSHERIRAFA 780
            DK  TL FSSFFC ILDC LPSDLAK SP YDVLFLLR IEGMNRMAFHIMSHERIRAF+
Sbjct: 954  DKGATLHFSSFFCGILDCDLPSDLAKESPVYDVLFLLRIIEGMNRMAFHIMSHERIRAFS 1013

Query: 781  EGKIDTLDNIKLSVPTVSQNEFVNSKLTEKLEQQMRDSSAVSVGGMPLWCKELMDSCPFL 840
            EG+I TL+NIKLSVP+VSQNEFVNSKLTEKLEQQMRD SAVS+GGMPLWCKELMDSCPFL
Sbjct: 1014 EGRISTLNNIKLSVPSVSQNEFVNSKLTEKLEQQMRDFSAVSIGGMPLWCKELMDSCPFL 1073

Query: 841  FSFEARRKYFRIVVFGMPQYQLHVRSHSDLGTSNDGRSSSGGLPRKKVLVHRDQILDSAS 900
            FSFEARRKYFRIVVFGMPQ Q +VRS+SDLGTSN  RSSSGG PRKK LV R+QIL SA+
Sbjct: 1074 FSFEARRKYFRIVVFGMPQ-QPYVRSYSDLGTSNGVRSSSGGFPRKKALVLRNQILLSAA 1133

Query: 901  KMMDQYAHQKVLLEVEYDEEVGTGLGPTLEFYTLVSREFQKYGLGMWRGDHDAFISGKRL 960
            KMMDQYAHQKVLLEVEYDEEVGTGLGPTLEFYTLVS+EFQKYGLGMWRGDHDAFI GK L
Sbjct: 1134 KMMDQYAHQKVLLEVEYDEEVGTGLGPTLEFYTLVSQEFQKYGLGMWRGDHDAFIPGKSL 1193

Query: 961  NIEGRETTESPFGLFPRPWPSTLDTDKLHLPEVMKKFVLLGQIVAKAIQDGRVLDIYFSK 1020
            NIEGRET ESPFGLFPRPW ST+D  +L   EV+KKF L+GQIVAKAIQDGRV+DIYFSK
Sbjct: 1194 NIEGRETIESPFGLFPRPWLSTVDIGELQFSEVIKKFTLMGQIVAKAIQDGRVMDIYFSK 1253

Query: 1021 SFYKLILGQELSIYDIQSFDPELGTVLLEFQSLVNRNKILGTVYEENSSSKLEFSYHNTN 1080
            +FYKLILGQE+SIYDIQSFDPELGTVLLEFQ+LVNR+K+L +V EENSSSKLEF YH+TN
Sbjct: 1254 AFYKLILGQEVSIYDIQSFDPELGTVLLEFQALVNRSKLLESVCEENSSSKLEFCYHDTN 1313

Query: 1081 IEDLCLDFTLPGYPDYLLTSSQDNSMVNAKNLEDYVSLVADATLYSGISRQIEAFKSGFN 1140
            IEDLCLDFTLPGYPD  LTSSQDNSMVN KNLEDYVSLVADATLYSGIS QIEAFKSGFN
Sbjct: 1314 IEDLCLDFTLPGYPDCRLTSSQDNSMVNTKNLEDYVSLVADATLYSGISTQIEAFKSGFN 1373

Query: 1141 QVFPIEHLQVFTAEELERLICGEQDIWALSDLLDNMKFDHGYTSSSPSIIHLLEIIQEFD 1200
            QVFPIEHLQVFTAEELERLICGEQD WALSDLLDN+KFDHGYT+SSPSII+LLEIIQ+FD
Sbjct: 1374 QVFPIEHLQVFTAEELERLICGEQDSWALSDLLDNIKFDHGYTASSPSIINLLEIIQDFD 1433

Query: 1201 NKQQRAFLQFVTGAPRLPSGGFASLNPKLTIVRKHSSNLVDYDLPSVMTCANYLKLPPYS 1260
            N+QQRAFLQFVTGAPRLPSGGFASLNPKLTIVRKHSSN+VD DLPSVMTCANYLKLPPYS
Sbjct: 1434 NEQQRAFLQFVTGAPRLPSGGFASLNPKLTIVRKHSSNMVDSDLPSVMTCANYLKLPPYS 1493

Query: 1261 SKEIMKEKLLYAITEGQGSFHLS 1283
            SKEIMKEKLLYAITEGQGSFHLS
Sbjct: 1494 SKEIMKEKLLYAITEGQGSFHLS 1514

BLAST of IVF0019533 vs. NCBI nr
Match: XP_008442639.1 (PREDICTED: E3 ubiquitin-protein ligase UPL4 [Cucumis melo])

HSP 1 Score: 2534 bits (6569), Expect = 0.0
Identity = 1282/1282 (100.00%), Postives = 1282/1282 (100.00%), Query Frame = 0

Query: 1    MAVLTFIDFFPTIIQRTALRIVVNVCKKLPSECPPNLIEAVPILCNLLQYDDEELVENVA 60
            MAVLTFIDFFPTIIQRTALRIVVNVCKKLPSECPPNLIEAVPILCNLLQYDDEELVENVA
Sbjct: 234  MAVLTFIDFFPTIIQRTALRIVVNVCKKLPSECPPNLIEAVPILCNLLQYDDEELVENVA 293

Query: 61   RCMIKIAECVHQSSELLDGLCQHGLIQHAIRLINLNSRTTLSQTIYNDLLGVLIKLASGS 120
            RCMIKIAECVHQSSELLDGLCQHGLIQHAIRLINLNSRTTLSQTIYNDLLGVLIKLASGS
Sbjct: 294  RCMIKIAECVHQSSELLDGLCQHGLIQHAIRLINLNSRTTLSQTIYNDLLGVLIKLASGS 353

Query: 121  IVAFETLYELNISNTLKDILSAYNLSHGVSSSCAVVDGQRNQVCEVLKLLNELLPTEDAK 180
            IVAFETLYELNISNTLKDILSAYNLSHGVSSSCAVVDGQRNQVCEVLKLLNELLPTEDAK
Sbjct: 354  IVAFETLYELNISNTLKDILSAYNLSHGVSSSCAVVDGQRNQVCEVLKLLNELLPTEDAK 413

Query: 181  TEQLSEKVSFLVGNPKQLQKFGLDILPLLVQVVSSGANLYVCCGCLTIIYKFVCLGESDM 240
            TEQLSEKVSFLVGNPKQLQKFGLDILPLLVQVVSSGANLYVCCGCLTIIYKFVCLGESDM
Sbjct: 414  TEQLSEKVSFLVGNPKQLQKFGLDILPLLVQVVSSGANLYVCCGCLTIIYKFVCLGESDM 473

Query: 241  LVELLENSNISSFLAGVFTRKDHHVLMLGLKITEIILQKLASIFLKSFVKEGVYFAIDAL 300
            LVELLENSNISSFLAGVFTRKDHHVLMLGLKITEIILQKLASIFLKSFVKEGVYFAIDAL
Sbjct: 474  LVELLENSNISSFLAGVFTRKDHHVLMLGLKITEIILQKLASIFLKSFVKEGVYFAIDAL 533

Query: 301  ISPEKYKQLIFPVFTGVHSSFDSCQKSSREHGKCLCYAFSSSCFPSGSETGSCKLDKDSV 360
            ISPEKYKQLIFPVFTGVHSSFDSCQKSSREHGKCLCYAFSSSCFPSGSETGSCKLDKDSV
Sbjct: 534  ISPEKYKQLIFPVFTGVHSSFDSCQKSSREHGKCLCYAFSSSCFPSGSETGSCKLDKDSV 593

Query: 361  YSLANHIRNIYFAEDLCDTDEGVTDILQNLRTFSGALDDLLNLSLIKDTPAQDEEKLYAL 420
            YSLANHIRNIYFAEDLCDTDEGVTDILQNLRTFSGALDDLLNLSLIKDTPAQDEEKLYAL
Sbjct: 594  YSLANHIRNIYFAEDLCDTDEGVTDILQNLRTFSGALDDLLNLSLIKDTPAQDEEKLYAL 653

Query: 421  LAEIMSKLKCGEPISTFEFIESGIVKSFINYITNGQYLRKKEEAQTISRQFSIIERRFEA 480
            LAEIMSKLKCGEPISTFEFIESGIVKSFINYITNGQYLRKKEEAQTISRQFSIIERRFEA
Sbjct: 654  LAEIMSKLKCGEPISTFEFIESGIVKSFINYITNGQYLRKKEEAQTISRQFSIIERRFEA 713

Query: 481  FARLLLSSSDHPSVNLPVLALIRKLQISLSSLENFPVISSQGFKHRNYFATVPNARCVPH 540
            FARLLLSSSDHPSVNLPVLALIRKLQISLSSLENFPVISSQGFKHRNYFATVPNARCVPH
Sbjct: 714  FARLLLSSSDHPSVNLPVLALIRKLQISLSSLENFPVISSQGFKHRNYFATVPNARCVPH 773

Query: 541  PCVKVRFVRGDGETKLCDITGDILTVDPFSSLAAIEGFLWRKVSTKKTEQSPEETLREHQ 600
            PCVKVRFVRGDGETKLCDITGDILTVDPFSSLAAIEGFLWRKVSTKKTEQSPEETLREHQ
Sbjct: 774  PCVKVRFVRGDGETKLCDITGDILTVDPFSSLAAIEGFLWRKVSTKKTEQSPEETLREHQ 833

Query: 601  IKLLSNIRSCLGVNSELVGSDIMSTDLPEVQVPAEVGADEKSQSSPSCSKKGTAPRLLLY 660
            IKLLSNIRSCLGVNSELVGSDIMSTDLPEVQVPAEVGADEKSQSSPSCSKKGTAPRLLLY
Sbjct: 834  IKLLSNIRSCLGVNSELVGSDIMSTDLPEVQVPAEVGADEKSQSSPSCSKKGTAPRLLLY 893

Query: 661  LEGKQLEPTMTIYQAILQQHIKENETISGTKVWSHVYTIMYRSAGEVEDNTCNQLFCASD 720
            LEGKQLEPTMTIYQAILQQHIKENETISGTKVWSHVYTIMYRSAGEVEDNTCNQLFCASD
Sbjct: 894  LEGKQLEPTMTIYQAILQQHIKENETISGTKVWSHVYTIMYRSAGEVEDNTCNQLFCASD 953

Query: 721  KAPTLQFSSFFCDILDCVLPSDLAKGSPAYDVLFLLRSIEGMNRMAFHIMSHERIRAFAE 780
            KAPTLQFSSFFCDILDCVLPSDLAKGSPAYDVLFLLRSIEGMNRMAFHIMSHERIRAFAE
Sbjct: 954  KAPTLQFSSFFCDILDCVLPSDLAKGSPAYDVLFLLRSIEGMNRMAFHIMSHERIRAFAE 1013

Query: 781  GKIDTLDNIKLSVPTVSQNEFVNSKLTEKLEQQMRDSSAVSVGGMPLWCKELMDSCPFLF 840
            GKIDTLDNIKLSVPTVSQNEFVNSKLTEKLEQQMRDSSAVSVGGMPLWCKELMDSCPFLF
Sbjct: 1014 GKIDTLDNIKLSVPTVSQNEFVNSKLTEKLEQQMRDSSAVSVGGMPLWCKELMDSCPFLF 1073

Query: 841  SFEARRKYFRIVVFGMPQYQLHVRSHSDLGTSNDGRSSSGGLPRKKVLVHRDQILDSASK 900
            SFEARRKYFRIVVFGMPQYQLHVRSHSDLGTSNDGRSSSGGLPRKKVLVHRDQILDSASK
Sbjct: 1074 SFEARRKYFRIVVFGMPQYQLHVRSHSDLGTSNDGRSSSGGLPRKKVLVHRDQILDSASK 1133

Query: 901  MMDQYAHQKVLLEVEYDEEVGTGLGPTLEFYTLVSREFQKYGLGMWRGDHDAFISGKRLN 960
            MMDQYAHQKVLLEVEYDEEVGTGLGPTLEFYTLVSREFQKYGLGMWRGDHDAFISGKRLN
Sbjct: 1134 MMDQYAHQKVLLEVEYDEEVGTGLGPTLEFYTLVSREFQKYGLGMWRGDHDAFISGKRLN 1193

Query: 961  IEGRETTESPFGLFPRPWPSTLDTDKLHLPEVMKKFVLLGQIVAKAIQDGRVLDIYFSKS 1020
            IEGRETTESPFGLFPRPWPSTLDTDKLHLPEVMKKFVLLGQIVAKAIQDGRVLDIYFSKS
Sbjct: 1194 IEGRETTESPFGLFPRPWPSTLDTDKLHLPEVMKKFVLLGQIVAKAIQDGRVLDIYFSKS 1253

Query: 1021 FYKLILGQELSIYDIQSFDPELGTVLLEFQSLVNRNKILGTVYEENSSSKLEFSYHNTNI 1080
            FYKLILGQELSIYDIQSFDPELGTVLLEFQSLVNRNKILGTVYEENSSSKLEFSYHNTNI
Sbjct: 1254 FYKLILGQELSIYDIQSFDPELGTVLLEFQSLVNRNKILGTVYEENSSSKLEFSYHNTNI 1313

Query: 1081 EDLCLDFTLPGYPDYLLTSSQDNSMVNAKNLEDYVSLVADATLYSGISRQIEAFKSGFNQ 1140
            EDLCLDFTLPGYPDYLLTSSQDNSMVNAKNLEDYVSLVADATLYSGISRQIEAFKSGFNQ
Sbjct: 1314 EDLCLDFTLPGYPDYLLTSSQDNSMVNAKNLEDYVSLVADATLYSGISRQIEAFKSGFNQ 1373

Query: 1141 VFPIEHLQVFTAEELERLICGEQDIWALSDLLDNMKFDHGYTSSSPSIIHLLEIIQEFDN 1200
            VFPIEHLQVFTAEELERLICGEQDIWALSDLLDNMKFDHGYTSSSPSIIHLLEIIQEFDN
Sbjct: 1374 VFPIEHLQVFTAEELERLICGEQDIWALSDLLDNMKFDHGYTSSSPSIIHLLEIIQEFDN 1433

Query: 1201 KQQRAFLQFVTGAPRLPSGGFASLNPKLTIVRKHSSNLVDYDLPSVMTCANYLKLPPYSS 1260
            KQQRAFLQFVTGAPRLPSGGFASLNPKLTIVRKHSSNLVDYDLPSVMTCANYLKLPPYSS
Sbjct: 1434 KQQRAFLQFVTGAPRLPSGGFASLNPKLTIVRKHSSNLVDYDLPSVMTCANYLKLPPYSS 1493

Query: 1261 KEIMKEKLLYAITEGQGSFHLS 1282
            KEIMKEKLLYAITEGQGSFHLS
Sbjct: 1494 KEIMKEKLLYAITEGQGSFHLS 1515

BLAST of IVF0019533 vs. NCBI nr
Match: TYK25103.1 (E3 ubiquitin-protein ligase UPL4 [Cucumis melo var. makuwa])

HSP 1 Score: 2530 bits (6557), Expect = 0.0
Identity = 1282/1283 (99.92%), Postives = 1282/1283 (99.92%), Query Frame = 0

Query: 1    MAVLTFIDFFPTIIQRTALRIVVNVCKKLPSECPPNLIEAVPILCNLLQYDDEELVENVA 60
            MAVLTFIDFFPTIIQRTALRIVVNVCKKLPSECPPNLIEAVPILCNLLQYDDEELVENVA
Sbjct: 234  MAVLTFIDFFPTIIQRTALRIVVNVCKKLPSECPPNLIEAVPILCNLLQYDDEELVENVA 293

Query: 61   RCMIKIAECVHQSSELLDGLCQHGLIQHAIRLINLNSRTTLSQTIYNDLLGVLIKLASGS 120
            RCMIKIAECVHQSSELLDGLCQHGLIQHAIRLINLNSRTTLSQTIYNDLLGVLIKLASGS
Sbjct: 294  RCMIKIAECVHQSSELLDGLCQHGLIQHAIRLINLNSRTTLSQTIYNDLLGVLIKLASGS 353

Query: 121  IVAFETLYELNISNTLKDILSAYNLSHGVSSSCAVVDGQRNQVCEVLKLLNELLPTEDAK 180
            IVAFETLYELNISNTLKDILSAYNLSHGVSSSCAVVDGQRNQVCEVLKLLNELLPTEDAK
Sbjct: 354  IVAFETLYELNISNTLKDILSAYNLSHGVSSSCAVVDGQRNQVCEVLKLLNELLPTEDAK 413

Query: 181  TEQLSEKVSFLVGNPKQLQKFGLDILPLLVQVV-SSGANLYVCCGCLTIIYKFVCLGESD 240
            TEQLSEKVSFLVGNPKQLQKFGLDILPLLVQVV SSGANLYVCCGCLTIIYKFVCLGESD
Sbjct: 414  TEQLSEKVSFLVGNPKQLQKFGLDILPLLVQVVVSSGANLYVCCGCLTIIYKFVCLGESD 473

Query: 241  MLVELLENSNISSFLAGVFTRKDHHVLMLGLKITEIILQKLASIFLKSFVKEGVYFAIDA 300
            MLVELLENSNISSFLAGVFTRKDHHVLMLGLKITEIILQKLASIFLKSFVKEGVYFAIDA
Sbjct: 474  MLVELLENSNISSFLAGVFTRKDHHVLMLGLKITEIILQKLASIFLKSFVKEGVYFAIDA 533

Query: 301  LISPEKYKQLIFPVFTGVHSSFDSCQKSSREHGKCLCYAFSSSCFPSGSETGSCKLDKDS 360
            LISPEKYKQLIFPVFTGVHSSFDSCQKSSREHGKCLCYAFSSSCFPSGSETGSCKLDKDS
Sbjct: 534  LISPEKYKQLIFPVFTGVHSSFDSCQKSSREHGKCLCYAFSSSCFPSGSETGSCKLDKDS 593

Query: 361  VYSLANHIRNIYFAEDLCDTDEGVTDILQNLRTFSGALDDLLNLSLIKDTPAQDEEKLYA 420
            VYSLANHIRNIYFAEDLCDTDEGVTDILQNLRTFSGALDDLLNLSLIKDTPAQDEEKLYA
Sbjct: 594  VYSLANHIRNIYFAEDLCDTDEGVTDILQNLRTFSGALDDLLNLSLIKDTPAQDEEKLYA 653

Query: 421  LLAEIMSKLKCGEPISTFEFIESGIVKSFINYITNGQYLRKKEEAQTISRQFSIIERRFE 480
            LLAEIMSKLKCGEPISTFEFIESGIVKSFINYITNGQYLRKKEEAQTISRQFSIIERRFE
Sbjct: 654  LLAEIMSKLKCGEPISTFEFIESGIVKSFINYITNGQYLRKKEEAQTISRQFSIIERRFE 713

Query: 481  AFARLLLSSSDHPSVNLPVLALIRKLQISLSSLENFPVISSQGFKHRNYFATVPNARCVP 540
            AFARLLLSSSDHPSVNLPVLALIRKLQISLSSLENFPVISSQGFKHRNYFATVPNARCVP
Sbjct: 714  AFARLLLSSSDHPSVNLPVLALIRKLQISLSSLENFPVISSQGFKHRNYFATVPNARCVP 773

Query: 541  HPCVKVRFVRGDGETKLCDITGDILTVDPFSSLAAIEGFLWRKVSTKKTEQSPEETLREH 600
            HPCVKVRFVRGDGETKLCDITGDILTVDPFSSLAAIEGFLWRKVSTKKTEQSPEETLREH
Sbjct: 774  HPCVKVRFVRGDGETKLCDITGDILTVDPFSSLAAIEGFLWRKVSTKKTEQSPEETLREH 833

Query: 601  QIKLLSNIRSCLGVNSELVGSDIMSTDLPEVQVPAEVGADEKSQSSPSCSKKGTAPRLLL 660
            QIKLLSNIRSCLGVNSELVGSDIMSTDLPEVQVPAEVGADEKSQSSPSCSKKGTAPRLLL
Sbjct: 834  QIKLLSNIRSCLGVNSELVGSDIMSTDLPEVQVPAEVGADEKSQSSPSCSKKGTAPRLLL 893

Query: 661  YLEGKQLEPTMTIYQAILQQHIKENETISGTKVWSHVYTIMYRSAGEVEDNTCNQLFCAS 720
            YLEGKQLEPTMTIYQAILQQHIKENETISGTKVWSHVYTIMYRSAGEVEDNTCNQLFCAS
Sbjct: 894  YLEGKQLEPTMTIYQAILQQHIKENETISGTKVWSHVYTIMYRSAGEVEDNTCNQLFCAS 953

Query: 721  DKAPTLQFSSFFCDILDCVLPSDLAKGSPAYDVLFLLRSIEGMNRMAFHIMSHERIRAFA 780
            DKAPTLQFSSFFCDILDCVLPSDLAKGSPAYDVLFLLRSIEGMNRMAFHIMSHERIRAFA
Sbjct: 954  DKAPTLQFSSFFCDILDCVLPSDLAKGSPAYDVLFLLRSIEGMNRMAFHIMSHERIRAFA 1013

Query: 781  EGKIDTLDNIKLSVPTVSQNEFVNSKLTEKLEQQMRDSSAVSVGGMPLWCKELMDSCPFL 840
            EGKIDTLDNIKLSVPTVSQNEFVNSKLTEKLEQQMRDSSAVSVGGMPLWCKELMDSCPFL
Sbjct: 1014 EGKIDTLDNIKLSVPTVSQNEFVNSKLTEKLEQQMRDSSAVSVGGMPLWCKELMDSCPFL 1073

Query: 841  FSFEARRKYFRIVVFGMPQYQLHVRSHSDLGTSNDGRSSSGGLPRKKVLVHRDQILDSAS 900
            FSFEARRKYFRIVVFGMPQYQLHVRSHSDLGTSNDGRSSSGGLPRKKVLVHRDQILDSAS
Sbjct: 1074 FSFEARRKYFRIVVFGMPQYQLHVRSHSDLGTSNDGRSSSGGLPRKKVLVHRDQILDSAS 1133

Query: 901  KMMDQYAHQKVLLEVEYDEEVGTGLGPTLEFYTLVSREFQKYGLGMWRGDHDAFISGKRL 960
            KMMDQYAHQKVLLEVEYDEEVGTGLGPTLEFYTLVSREFQKYGLGMWRGDHDAFISGKRL
Sbjct: 1134 KMMDQYAHQKVLLEVEYDEEVGTGLGPTLEFYTLVSREFQKYGLGMWRGDHDAFISGKRL 1193

Query: 961  NIEGRETTESPFGLFPRPWPSTLDTDKLHLPEVMKKFVLLGQIVAKAIQDGRVLDIYFSK 1020
            NIEGRETTESPFGLFPRPWPSTLDTDKLHLPEVMKKFVLLGQIVAKAIQDGRVLDIYFSK
Sbjct: 1194 NIEGRETTESPFGLFPRPWPSTLDTDKLHLPEVMKKFVLLGQIVAKAIQDGRVLDIYFSK 1253

Query: 1021 SFYKLILGQELSIYDIQSFDPELGTVLLEFQSLVNRNKILGTVYEENSSSKLEFSYHNTN 1080
            SFYKLILGQELSIYDIQSFDPELGTVLLEFQSLVNRNKILGTVYEENSSSKLEFSYHNTN
Sbjct: 1254 SFYKLILGQELSIYDIQSFDPELGTVLLEFQSLVNRNKILGTVYEENSSSKLEFSYHNTN 1313

Query: 1081 IEDLCLDFTLPGYPDYLLTSSQDNSMVNAKNLEDYVSLVADATLYSGISRQIEAFKSGFN 1140
            IEDLCLDFTLPGYPDYLLTSSQDNSMVNAKNLEDYVSLVADATLYSGISRQIEAFKSGFN
Sbjct: 1314 IEDLCLDFTLPGYPDYLLTSSQDNSMVNAKNLEDYVSLVADATLYSGISRQIEAFKSGFN 1373

Query: 1141 QVFPIEHLQVFTAEELERLICGEQDIWALSDLLDNMKFDHGYTSSSPSIIHLLEIIQEFD 1200
            QVFPIEHLQVFTAEELERLICGEQDIWALSDLLDNMKFDHGYTSSSPSIIHLLEIIQEFD
Sbjct: 1374 QVFPIEHLQVFTAEELERLICGEQDIWALSDLLDNMKFDHGYTSSSPSIIHLLEIIQEFD 1433

Query: 1201 NKQQRAFLQFVTGAPRLPSGGFASLNPKLTIVRKHSSNLVDYDLPSVMTCANYLKLPPYS 1260
            NKQQRAFLQFVTGAPRLPSGGFASLNPKLTIVRKHSSNLVDYDLPSVMTCANYLKLPPYS
Sbjct: 1434 NKQQRAFLQFVTGAPRLPSGGFASLNPKLTIVRKHSSNLVDYDLPSVMTCANYLKLPPYS 1493

Query: 1261 SKEIMKEKLLYAITEGQGSFHLS 1282
            SKEIMKEKLLYAITEGQGSFHLS
Sbjct: 1494 SKEIMKEKLLYAITEGQGSFHLS 1516

BLAST of IVF0019533 vs. NCBI nr
Match: KAA0044037.1 (E3 ubiquitin-protein ligase UPL4 [Cucumis melo var. makuwa])

HSP 1 Score: 2520 bits (6532), Expect = 0.0
Identity = 1282/1308 (98.01%), Postives = 1282/1308 (98.01%), Query Frame = 0

Query: 1    MAVLTFIDFFPTIIQRTALRIVVNVCKKLPSECPPNLIEAVPILCNLLQYDDEELVENVA 60
            MAVLTFIDFFPTIIQRTALRIVVNVCKKLPSECPPNLIEAVPILCNLLQYDDEELVENVA
Sbjct: 224  MAVLTFIDFFPTIIQRTALRIVVNVCKKLPSECPPNLIEAVPILCNLLQYDDEELVENVA 283

Query: 61   RCMIKIAECVHQSSELLDGLCQHGLIQHAIRLINLNSRTTLSQTIYNDLLGVLIKLASGS 120
            RCMIKIAECVHQSSELLDGLCQHGLIQHAIRLINLNSRTTLSQTIYNDLLGVLIKLASGS
Sbjct: 284  RCMIKIAECVHQSSELLDGLCQHGLIQHAIRLINLNSRTTLSQTIYNDLLGVLIKLASGS 343

Query: 121  IVAFETLYELNISNTLKDILSAYNLSHGVSSSCAVVDGQRNQVCEVLKLLNELLPTEDAK 180
            IVAFETLYELNISNTLKDILSAYNLSHGVSSSCAVVDGQRNQVCEVLKLLNELLPTEDAK
Sbjct: 344  IVAFETLYELNISNTLKDILSAYNLSHGVSSSCAVVDGQRNQVCEVLKLLNELLPTEDAK 403

Query: 181  TEQLSEKVSFLVGNPKQLQKFGLDILPLLVQVVSSGANLYVCCGCLTIIYKFVCLGESDM 240
            TEQLSEKVSFLVGNPKQLQKFGLDILPLLVQVVSSGANLYVCCGCLTIIYKFVCLGESDM
Sbjct: 404  TEQLSEKVSFLVGNPKQLQKFGLDILPLLVQVVSSGANLYVCCGCLTIIYKFVCLGESDM 463

Query: 241  LVELLENSNISSFLAGVFTRKDHHVLMLGLKITEIILQKLASIFLKSFVKEGVYFAIDAL 300
            LVELLENSNISSFLAGVFTRKDHHVLMLGLKITEIILQKLASIFLKSFVKEGVYFAIDAL
Sbjct: 464  LVELLENSNISSFLAGVFTRKDHHVLMLGLKITEIILQKLASIFLKSFVKEGVYFAIDAL 523

Query: 301  ISPEKYKQLIFPVFTGVHSSFDSCQKSSREHGKCLCYAFSSSCFPSGSETGSCKLDKDSV 360
            ISPEKYKQLIFPVFTGVHSSFDSCQKSSREHGKCLCYAFSSSCFPSGSETGSCKLDKDSV
Sbjct: 524  ISPEKYKQLIFPVFTGVHSSFDSCQKSSREHGKCLCYAFSSSCFPSGSETGSCKLDKDSV 583

Query: 361  YSLANHIRNIYFAEDLCDTDEGVTDILQNLRTFSGALDDLLNLSLIKDTPAQDEEKLYAL 420
            YSLANHIRNIYFAEDLCDTDEGVTDILQNLRTFSGALDDLLNLSLIKDTPAQDEEKLYAL
Sbjct: 584  YSLANHIRNIYFAEDLCDTDEGVTDILQNLRTFSGALDDLLNLSLIKDTPAQDEEKLYAL 643

Query: 421  LAEIMSKLKCGEPISTFEFIESGIVKSFINYITNGQYLRKKEEAQTISRQFSIIERRFEA 480
            LAEIMSKLKCGEPISTFEFIESGIVKSFINYITNGQYLRKKEEAQTISRQFSIIERRFEA
Sbjct: 644  LAEIMSKLKCGEPISTFEFIESGIVKSFINYITNGQYLRKKEEAQTISRQFSIIERRFEA 703

Query: 481  FARLLLSSSDHPSVNLPVLALIRKLQISLSSLENFPVISSQGFKHRNYFATVPNARCVPH 540
            FARLLLSSSDHPSVNLPVLALIRKLQISLSSLENFPVISSQGFKHRNYFATVPNARCVPH
Sbjct: 704  FARLLLSSSDHPSVNLPVLALIRKLQISLSSLENFPVISSQGFKHRNYFATVPNARCVPH 763

Query: 541  PCVKVRFVRGDGETKLCDITGDILTVDPFSSLAAIEGFLWRKVSTKKTEQSPEETLREHQ 600
            PCVKVRFVRGDGETKLCDITGDILTVDPFSSLAAIEGFLWRKVSTKKTEQSPEETLREHQ
Sbjct: 764  PCVKVRFVRGDGETKLCDITGDILTVDPFSSLAAIEGFLWRKVSTKKTEQSPEETLREHQ 823

Query: 601  IKLLSNIRSCLGVNSELVGSDIMSTDLPEVQVPAEVGADEKSQSSPSCSKKGTAPRLLLY 660
            IKLLSNIRSCLGVNSELVGSDIMSTDLPEVQVPAEVGADEKSQSSPSCSKKGTAPRLLLY
Sbjct: 824  IKLLSNIRSCLGVNSELVGSDIMSTDLPEVQVPAEVGADEKSQSSPSCSKKGTAPRLLLY 883

Query: 661  LEGKQLEPTMTIYQAILQQHIKENETISGTKVWSHVYTIMYRSAGEVEDNTCNQLFCASD 720
            LEGKQLEPTMTIYQAILQQHIKENETISGTKVWSHVYTIMYRSAGEVEDNTCNQLFCASD
Sbjct: 884  LEGKQLEPTMTIYQAILQQHIKENETISGTKVWSHVYTIMYRSAGEVEDNTCNQLFCASD 943

Query: 721  KAPTLQFSSFFCDILDCVLPSDLAKGSPAYDVLFLLRSIEGMNRMAFHIMSHERIRAFAE 780
            KAPTLQFSSFFCDILDCVLPSDLAKGSPAYDVLFLLRSIEGMNRMAFHIMSHERIRAFAE
Sbjct: 944  KAPTLQFSSFFCDILDCVLPSDLAKGSPAYDVLFLLRSIEGMNRMAFHIMSHERIRAFAE 1003

Query: 781  GKIDTLDNIKLSVPTVSQNEFVNSKLTEKLEQQMRDSSAVSVGGMPLWCKELMDSCPFLF 840
            GKIDTLDNIKLSVPTVSQNEFVNSKLTEKLEQQMRDSSAVSVGGMPLWCKELMDSCPFLF
Sbjct: 1004 GKIDTLDNIKLSVPTVSQNEFVNSKLTEKLEQQMRDSSAVSVGGMPLWCKELMDSCPFLF 1063

Query: 841  SFEARRKYFRIVVFGMPQYQLHVRSHSDLGTSNDGRSSSGGLPRKKVLVHRDQILDSASK 900
            SFEARRKYFRIVVFGMPQYQLHVRSHSDLGTSNDGRSSSGGLPRKKVLVHRDQILDSASK
Sbjct: 1064 SFEARRKYFRIVVFGMPQYQLHVRSHSDLGTSNDGRSSSGGLPRKKVLVHRDQILDSASK 1123

Query: 901  MMDQYAHQKVLLEVEYDEEVGTGLGPTLEFYTLVSREFQKYGLGMWRGDHDAFISGKRLN 960
            MMDQYAHQKVLLEVEYDEEVGTGLGPTLEFYTLVSREFQKYGLGMWRGDHDAFISGKRLN
Sbjct: 1124 MMDQYAHQKVLLEVEYDEEVGTGLGPTLEFYTLVSREFQKYGLGMWRGDHDAFISGKRLN 1183

Query: 961  IEGRETTESPFGLFPRPWPSTLDTDKLHLPEVMKKFVLLGQIVAKAIQDGRVLDIYFSKS 1020
            IEGRETTESPFGLFPRPWPSTLDTDKLHLPEVMKKFVLLGQIVAKAIQDGRVLDIYFSKS
Sbjct: 1184 IEGRETTESPFGLFPRPWPSTLDTDKLHLPEVMKKFVLLGQIVAKAIQDGRVLDIYFSKS 1243

Query: 1021 FYKLILGQELSIYDIQSFDPELGTVLLEFQSLVNRNKILGTVYEENSSSKLEFSYHNTNI 1080
            FYKLILGQELSIYDIQSFDPELGTVLLEFQSLVNRNKILGTVYEENSSSKLEFSYHNTNI
Sbjct: 1244 FYKLILGQELSIYDIQSFDPELGTVLLEFQSLVNRNKILGTVYEENSSSKLEFSYHNTNI 1303

Query: 1081 EDLCLDFTLPGYPDYLLTSSQDNSMVNAKNLEDYVSLVADATLYSGISRQIEAFKSGFNQ 1140
            EDLCLDFTLPGYPDYLLTSSQDNSMVNAKNLEDYVSLVADATLYSGISRQIEAFKSGFNQ
Sbjct: 1304 EDLCLDFTLPGYPDYLLTSSQDNSMVNAKNLEDYVSLVADATLYSGISRQIEAFKSGFNQ 1363

Query: 1141 VFPIEHLQVFTAEELERLICGEQDIWA--------------------------LSDLLDN 1200
            VFPIEHLQVFTAEELERLICGEQDIWA                          LSDLLDN
Sbjct: 1364 VFPIEHLQVFTAEELERLICGEQDIWAVRISANYHIYLKLLTISLVLFNLLMQLSDLLDN 1423

Query: 1201 MKFDHGYTSSSPSIIHLLEIIQEFDNKQQRAFLQFVTGAPRLPSGGFASLNPKLTIVRKH 1260
            MKFDHGYTSSSPSIIHLLEIIQEFDNKQQRAFLQFVTGAPRLPSGGFASLNPKLTIVRKH
Sbjct: 1424 MKFDHGYTSSSPSIIHLLEIIQEFDNKQQRAFLQFVTGAPRLPSGGFASLNPKLTIVRKH 1483

Query: 1261 SSNLVDYDLPSVMTCANYLKLPPYSSKEIMKEKLLYAITEGQGSFHLS 1282
            SSNLVDYDLPSVMTCANYLKLPPYSSKEIMKEKLLYAITEGQGSFHLS
Sbjct: 1484 SSNLVDYDLPSVMTCANYLKLPPYSSKEIMKEKLLYAITEGQGSFHLS 1531

BLAST of IVF0019533 vs. NCBI nr
Match: XP_004137960.2 (E3 ubiquitin-protein ligase UPL4 [Cucumis sativus] >KGN58932.1 hypothetical protein Csa_001282 [Cucumis sativus])

HSP 1 Score: 2393 bits (6202), Expect = 0.0
Identity = 1218/1283 (94.93%), Postives = 1242/1283 (96.80%), Query Frame = 0

Query: 1    MAVLTFIDFFPTIIQRTALRIVVNVCKKLPSECPPNLIEAVPILCNLLQYDDEELVENVA 60
            MAVLTFIDFFPTIIQRTALRIVVNVCKKLPSECP NLIEAVPILCNLLQYDDEELVENVA
Sbjct: 234  MAVLTFIDFFPTIIQRTALRIVVNVCKKLPSECPQNLIEAVPILCNLLQYDDEELVENVA 293

Query: 61   RCMIKIAECVHQSSELLDGLCQHGLIQHAIRLINLNSRTTLSQTIYNDLLGVLIKLASGS 120
            RCMIKIAECVHQSSELLDGLCQHGLIQHAIRLINLNSRTTLSQTIYNDLLGVLIKLASGS
Sbjct: 294  RCMIKIAECVHQSSELLDGLCQHGLIQHAIRLINLNSRTTLSQTIYNDLLGVLIKLASGS 353

Query: 121  IVAFETLYELNISNTLKDILSAYNLSHGVSSSCAVVDGQRNQVCEVLKLLNELLPTEDAK 180
            IVAFETLYELNISNTLKDILSAYNLSHGVSSSCAVVDGQRNQVCEVLKLLNELLPTEDAK
Sbjct: 354  IVAFETLYELNISNTLKDILSAYNLSHGVSSSCAVVDGQRNQVCEVLKLLNELLPTEDAK 413

Query: 181  TEQLSEKVSFLVGNPKQLQKFGLDILPLLVQVVSSGANLYVCCGCLTIIYKFVCLGESDM 240
            TEQLSEKVSFLV NPKQLQKFGLDILPLLVQVVSSGANLYVCCGCLTIIYKFVCLGESDM
Sbjct: 414  TEQLSEKVSFLVSNPKQLQKFGLDILPLLVQVVSSGANLYVCCGCLTIIYKFVCLGESDM 473

Query: 241  LVELLENSNISSFLAGVFTRKDHHVLMLGLKITEIILQKLASIFLKSFVKEGVYFAIDAL 300
            LVELLENSNISSFLAGVFTRKDHHVLMLGLKITEIILQKLAS FLKSFVKEGVYF+IDAL
Sbjct: 474  LVELLENSNISSFLAGVFTRKDHHVLMLGLKITEIILQKLASTFLKSFVKEGVYFSIDAL 533

Query: 301  ISPEKYKQLIFPVFTGVHSSFDSCQKSSREHGKCLCYAFSSSCFPSGSETGSCKLDKDSV 360
            ISP+KYKQLIFPVFTGVHSSF SCQKSSREHG+CLCYAFSSSCFPS SETGSCKLDKDSV
Sbjct: 534  ISPDKYKQLIFPVFTGVHSSFGSCQKSSREHGRCLCYAFSSSCFPSVSETGSCKLDKDSV 593

Query: 361  YSLANHIRNIYFAEDLCDTDEGVTDILQNLRTFSGALDDLLNLSLIKDTPAQDEEKLYAL 420
            YSLANHIR+IYFAEDLCDTDEGVTDILQNLRTFSGALDDLLNLSLIKDTPAQDEEKLYAL
Sbjct: 594  YSLANHIRSIYFAEDLCDTDEGVTDILQNLRTFSGALDDLLNLSLIKDTPAQDEEKLYAL 653

Query: 421  LAEIMSKLKCGEPISTFEFIESGIVKSFINYITNGQYLRKKEEAQTISRQFSIIERRFEA 480
            LAEIMSKLKCGEPISTFEFIESGIVKSFINYITNGQYLRKK E+Q ISR FSIIERRFEA
Sbjct: 654  LAEIMSKLKCGEPISTFEFIESGIVKSFINYITNGQYLRKKGESQPISRHFSIIERRFEA 713

Query: 481  FARLLLSSSDHPSVNLPVLALIRKLQISLSSLENFPVI-SSQGFKHRNYFATVPNARCVP 540
            FARLLLSSSDHPSVNLPVLALIRKLQISLSSLENF VI SSQGFKHRNYF TVPNARCVP
Sbjct: 714  FARLLLSSSDHPSVNLPVLALIRKLQISLSSLENFHVIISSQGFKHRNYFVTVPNARCVP 773

Query: 541  HPCVKVRFVRGDGETKLCDITGDILTVDPFSSLAAIEGFLWRKVSTKKTEQSPEETLREH 600
            HPCVKVRFVRGDGET LCDI GDIL VDPFSSL AIEGFLW KVS++KTEQSPE+TLREH
Sbjct: 774  HPCVKVRFVRGDGETDLCDINGDILNVDPFSSLTAIEGFLWPKVSSQKTEQSPEDTLREH 833

Query: 601  QIKLLSNIRSCLGVNSELVGSDIMSTDLPEVQVPAEVGADEKSQSSPSCSKKGTAPRLLL 660
            QIKLLS          +LVGSDIMSTDLPEVQVPAEV ADEKSQ S SCSKKGTAPRLLL
Sbjct: 834  QIKLLS----------KLVGSDIMSTDLPEVQVPAEVSADEKSQCSASCSKKGTAPRLLL 893

Query: 661  YLEGKQLEPTMTIYQAILQQHIKENETISGTKVWSHVYTIMYRSAGEVEDNTCNQLFCAS 720
            YLEGKQLEPT++IYQAILQQHIKENETISG K+WS VYTIMYRSAGEVED+TCNQLFCAS
Sbjct: 894  YLEGKQLEPTLSIYQAILQQHIKENETISGIKIWSQVYTIMYRSAGEVEDSTCNQLFCAS 953

Query: 721  DKAPTLQFSSFFCDILDCVLPSDLAKGSPAYDVLFLLRSIEGMNRMAFHIMSHERIRAFA 780
            DKA  LQFSSFFCDILDCVLPSDLAKGSPAYDVLFLLRSIEGMNRMAFHIMSHERIRAFA
Sbjct: 954  DKALKLQFSSFFCDILDCVLPSDLAKGSPAYDVLFLLRSIEGMNRMAFHIMSHERIRAFA 1013

Query: 781  EGKIDTLDNIKLSVPTVSQNEFVNSKLTEKLEQQMRDSSAVSVGGMPLWCKELMDSCPFL 840
            +GKIDTLDNIKLSVP+VSQNEFVNSKLTEKLEQQMRD SAVSVGGMPLWCKELMDSCPFL
Sbjct: 1014 DGKIDTLDNIKLSVPSVSQNEFVNSKLTEKLEQQMRDFSAVSVGGMPLWCKELMDSCPFL 1073

Query: 841  FSFEARRKYFRIVVFGMPQYQLHVRSHSDLGTSNDGRSSSGGLPRKKVLVHRDQILDSAS 900
            FSFEARRKYFRIVVFGMPQYQLH RSHSD GTSNDGRSSSGGLPRKKVLVHR QILDSAS
Sbjct: 1074 FSFEARRKYFRIVVFGMPQYQLHARSHSDFGTSNDGRSSSGGLPRKKVLVHRSQILDSAS 1133

Query: 901  KMMDQYAHQKVLLEVEYDEEVGTGLGPTLEFYTLVSREFQKYGLGMWRGDHDAFISGKRL 960
            KMM+QYA+QKVLLEVEYDEEVGTGLGPTLEFYTLVSREFQK GLGMWRGDHDAFISGKRL
Sbjct: 1134 KMMNQYANQKVLLEVEYDEEVGTGLGPTLEFYTLVSREFQKNGLGMWRGDHDAFISGKRL 1193

Query: 961  NIEGRETTESPFGLFPRPWPSTLDTDKLHLPEVMKKFVLLGQIVAKAIQDGRVLDIYFSK 1020
            NIE RETTESPFGLFPRPWPSTLDTDKLHLPEVMKKFVLLGQIVAKAIQD RVLDIYFSK
Sbjct: 1194 NIEDRETTESPFGLFPRPWPSTLDTDKLHLPEVMKKFVLLGQIVAKAIQDCRVLDIYFSK 1253

Query: 1021 SFYKLILGQELSIYDIQSFDPELGTVLLEFQSLVNRNKILGTVYEENSSSKLEFSYHNTN 1080
            +FYKLILGQELSIYDIQSFDPELGTVLLEFQ+LVNRNK+LG+VYEENSSSKLEFSYHNTN
Sbjct: 1254 AFYKLILGQELSIYDIQSFDPELGTVLLEFQALVNRNKLLGSVYEENSSSKLEFSYHNTN 1313

Query: 1081 IEDLCLDFTLPGYPDYLLTSSQDNSMVNAKNLEDYVSLVADATLYSGISRQIEAFKSGFN 1140
            IEDLCLDFTLPGYPDYLLTSSQDNSMVNAKNLE+YVSLVADATL SGISRQIEAFKSGFN
Sbjct: 1314 IEDLCLDFTLPGYPDYLLTSSQDNSMVNAKNLENYVSLVADATLCSGISRQIEAFKSGFN 1373

Query: 1141 QVFPIEHLQVFTAEELERLICGEQDIWALSDLLDNMKFDHGYTSSSPSIIHLLEIIQEFD 1200
            QVFPIEHLQVFTAEELERLICGEQDIWALSDLLDNMKFDHGYTSSSPSI+HLLEIIQ+FD
Sbjct: 1374 QVFPIEHLQVFTAEELERLICGEQDIWALSDLLDNMKFDHGYTSSSPSIVHLLEIIQDFD 1433

Query: 1201 NKQQRAFLQFVTGAPRLPSGGFASLNPKLTIVRKHSSNLVDYDLPSVMTCANYLKLPPYS 1260
            NKQQRAFLQFVTGAPRLPSGGFASLNPKLTIVRKHSSNLVDYDLPSVMTCANYLKLPPYS
Sbjct: 1434 NKQQRAFLQFVTGAPRLPSGGFASLNPKLTIVRKHSSNLVDYDLPSVMTCANYLKLPPYS 1493

Query: 1261 SKEIMKEKLLYAITEGQGSFHLS 1282
            SKEIMKEKLLYAITEGQGSFHLS
Sbjct: 1494 SKEIMKEKLLYAITEGQGSFHLS 1506

BLAST of IVF0019533 vs. NCBI nr
Match: XP_038904018.1 (E3 ubiquitin-protein ligase UPL4 [Benincasa hispida])

HSP 1 Score: 2329 bits (6036), Expect = 0.0
Identity = 1173/1283 (91.43%), Postives = 1222/1283 (95.25%), Query Frame = 0

Query: 1    MAVLTFIDFFPTIIQRTALRIVVNVCKKLPSECPPNLIEAVPILCNLLQYDDEELVENVA 60
            MAVLTFIDFFPTIIQRTALRIVVNVCKKLPSECPPNLIEAVPILCNLLQYDD ELVENVA
Sbjct: 234  MAVLTFIDFFPTIIQRTALRIVVNVCKKLPSECPPNLIEAVPILCNLLQYDDGELVENVA 293

Query: 61   RCMIKIAECVHQSSELLDGLCQHGLIQHAIRLINLNSRTTLSQTIYNDLLGVLIKLASGS 120
            RCMIKIAEC  QS ELLDGLC+HGLIQ AIRLINLNSRTTLSQT+YNDLLGVLIKL+SGS
Sbjct: 294  RCMIKIAECTQQSCELLDGLCRHGLIQQAIRLINLNSRTTLSQTVYNDLLGVLIKLSSGS 353

Query: 121  IVAFETLYELNISNTLKDILSAYNLSHGVSSSCAVVDGQRNQVCEVLKLLNELLPTEDAK 180
            IVAFETLYELNISNTLKDILS YNLSHGVSSSCAVVDGQRNQVCEVL LLNELLPTEDAK
Sbjct: 354  IVAFETLYELNISNTLKDILSVYNLSHGVSSSCAVVDGQRNQVCEVLTLLNELLPTEDAK 413

Query: 181  TEQLSEKVSFLVGNPKQLQKFGLDILPLLVQVVSSGANLYVCCGCLTIIYKFVCLGESDM 240
            TEQLSEKVSFL  NPKQLQKFGLDILPLLVQVVSSGANLYVCCGCLTIIYKFVCLGESDM
Sbjct: 414  TEQLSEKVSFLASNPKQLQKFGLDILPLLVQVVSSGANLYVCCGCLTIIYKFVCLGESDM 473

Query: 241  LVELLENSNISSFLAGVFTRKDHHVLMLGLKITEIILQKLASIFLKSFVKEGVYFAIDAL 300
            LVELL+N+NISSFLAGVFTRKDHHVLML LKITEIILQKL+SIFLKSFVKEGVYFAIDAL
Sbjct: 474  LVELLQNANISSFLAGVFTRKDHHVLMLALKITEIILQKLSSIFLKSFVKEGVYFAIDAL 533

Query: 301  ISPEKYKQLIFPVFTGVHSSFDSCQKSSREHGKCLCYAFSSSCFPSGSETGSCKLDKDSV 360
            I+PEKYKQLIFPVFTGVHSSF SCQKSSRE+G+CLCYAFSSSCF S +ETGSCKLDKDSV
Sbjct: 534  ITPEKYKQLIFPVFTGVHSSFGSCQKSSREYGRCLCYAFSSSCFSSAAETGSCKLDKDSV 593

Query: 361  YSLANHIRNIYFAEDLCDTDEGVTDILQNLRTFSGALDDLLNLSLIKDTPAQDEEKLYAL 420
            YSLANHIRN YF E+LCDTD+GVTDILQNLRTFS ALDDLL+LSLIKDTPAQDEEK YAL
Sbjct: 594  YSLANHIRNKYFTEELCDTDKGVTDILQNLRTFSAALDDLLSLSLIKDTPAQDEEKFYAL 653

Query: 421  LAEIMSKLKCGEPISTFEFIESGIVKSFINYITNGQYLRKKEEAQTISRQFSIIERRFEA 480
            LAEIMSKLKCGEPISTFEFIESGIVKSFINYITNGQYL KK E QTISRQFS+IERRFEA
Sbjct: 654  LAEIMSKLKCGEPISTFEFIESGIVKSFINYITNGQYLIKKGEPQTISRQFSVIERRFEA 713

Query: 481  FARLLLSSSDHPSVNLPVLALIRKLQISLSSLENFPVISSQGFKHRNYFATVPNARCVPH 540
            FARLLLSSSDHPSV LP+L LIRKLQISLSSLENFPVI SQGFKHRNYFATVPNARC+PH
Sbjct: 714  FARLLLSSSDHPSVKLPILVLIRKLQISLSSLENFPVILSQGFKHRNYFATVPNARCIPH 773

Query: 541  PCVKVRFVRGDGETKLCDITGDILTVDPFSSLAAIEGFLWRKVSTKKTEQSPE-ETLREH 600
            PC+KVRFVRGDGET LCDITGDIL+VDPFSSL AIEGFLW KV TKK EQS E ++LREH
Sbjct: 774  PCLKVRFVRGDGETDLCDITGDILSVDPFSSLNAIEGFLWPKVGTKKAEQSSEADSLREH 833

Query: 601  QIKLLSNIRSCLGVNSELVGSDIMSTDLPEVQVPAEVGADEKSQSSPSCSKKGTAPRLLL 660
            Q+KLLS++RSCLGVNSE V SD MSTDLPE+QV AEV  DEKSQ S SCSKKGT P+L L
Sbjct: 834  QVKLLSDVRSCLGVNSEFVESDNMSTDLPEIQVSAEVSVDEKSQCSASCSKKGTTPKLFL 893

Query: 661  YLEGKQLEPTMTIYQAILQQHIKENETISGTKVWSHVYTIMYRSAGEVEDNTCNQLFCAS 720
            YLEGKQLEPT+T+YQAILQQHIKENETISGTKVWS VYTI Y+S GEVEDN+CNQLF AS
Sbjct: 894  YLEGKQLEPTLTLYQAILQQHIKENETISGTKVWSRVYTITYKSTGEVEDNSCNQLFSAS 953

Query: 721  DKAPTLQFSSFFCDILDCVLPSDLAKGSPAYDVLFLLRSIEGMNRMAFHIMSHERIRAFA 780
            DKAP LQFSSFFC ILDCVLPSDLAKGSPAYDVLFLLRSIEGMNRMAFHIMSHERIRAF+
Sbjct: 954  DKAPMLQFSSFFCGILDCVLPSDLAKGSPAYDVLFLLRSIEGMNRMAFHIMSHERIRAFS 1013

Query: 781  EGKIDTLDNIKLSVPTVSQNEFVNSKLTEKLEQQMRDSSAVSVGGMPLWCKELMDSCPFL 840
            EG+I+TLDNIKLSVP+VSQNEFVNSKLTEKLEQQMRD SAVS+GGMPLWCKELMDSCPFL
Sbjct: 1014 EGRINTLDNIKLSVPSVSQNEFVNSKLTEKLEQQMRDFSAVSIGGMPLWCKELMDSCPFL 1073

Query: 841  FSFEARRKYFRIVVFGMPQYQLHVRSHSDLGTSNDGRSSSGGLPRKKVLVHRDQILDSAS 900
            FSFEARRKYFRIVVFGMPQYQLHVRSHSDLGTSND RSSSGGLPRKKVLVHR+QILDSA+
Sbjct: 1074 FSFEARRKYFRIVVFGMPQYQLHVRSHSDLGTSNDVRSSSGGLPRKKVLVHRNQILDSAA 1133

Query: 901  KMMDQYAHQKVLLEVEYDEEVGTGLGPTLEFYTLVSREFQKYGLGMWRGDHDAFISGKRL 960
            KMM+QYAHQKVLLEVEYDEEVGTGLGPTLEFYTLVSREFQKYGLGMWRGDHDAFIS K L
Sbjct: 1134 KMMNQYAHQKVLLEVEYDEEVGTGLGPTLEFYTLVSREFQKYGLGMWRGDHDAFISKKSL 1193

Query: 961  NIEGRETTESPFGLFPRPWPSTLDTDKLHLPEVMKKFVLLGQIVAKAIQDGRVLDIYFSK 1020
            NIEGRET  SPFGLFPRPWPSTLDTD+LH  EVMKKFVLLGQIVAKAIQDGRVLDIYFSK
Sbjct: 1194 NIEGRETIGSPFGLFPRPWPSTLDTDELHFSEVMKKFVLLGQIVAKAIQDGRVLDIYFSK 1253

Query: 1021 SFYKLILGQELSIYDIQSFDPELGTVLLEFQSLVNRNKILGTVYEENSSSKLEFSYHNTN 1080
            +FYKLILGQE+SIYDIQSFDPELGT+LLEF++LVNR+K+L +VYEENSSSKLE  YHNTN
Sbjct: 1254 TFYKLILGQEVSIYDIQSFDPELGTILLEFEALVNRSKLLESVYEENSSSKLELYYHNTN 1313

Query: 1081 IEDLCLDFTLPGYPDYLLTSSQDNSMVNAKNLEDYVSLVADATLYSGISRQIEAFKSGFN 1140
            IEDLCLDFTLPGYPDYLLTSSQDNSMVN KNLEDYVSLV DATL SGISRQIEAFKSGFN
Sbjct: 1314 IEDLCLDFTLPGYPDYLLTSSQDNSMVNTKNLEDYVSLVTDATLCSGISRQIEAFKSGFN 1373

Query: 1141 QVFPIEHLQVFTAEELERLICGEQDIWALSDLLDNMKFDHGYTSSSPSIIHLLEIIQEFD 1200
            QVFPIEHLQVFTAEELERLICGEQD WALS+LLDNMKFDHGYTSSSPSIIHLLEIIQEFD
Sbjct: 1374 QVFPIEHLQVFTAEELERLICGEQDSWALSELLDNMKFDHGYTSSSPSIIHLLEIIQEFD 1433

Query: 1201 NKQQRAFLQFVTGAPRLPSGGFASLNPKLTIVRKHSSNLVDYDLPSVMTCANYLKLPPYS 1260
            N+QQRAFLQFVTGAPRLPSGGFASLNPKLTIVRKHS+NLVDYDLPSVMTCANYLKLPPYS
Sbjct: 1434 NEQQRAFLQFVTGAPRLPSGGFASLNPKLTIVRKHSNNLVDYDLPSVMTCANYLKLPPYS 1493

Query: 1261 SKEIMKEKLLYAITEGQGSFHLS 1282
            SKEIMKEKLLYAITEGQGSFHLS
Sbjct: 1494 SKEIMKEKLLYAITEGQGSFHLS 1516

BLAST of IVF0019533 vs. TAIR 10
Match: AT5G02880.1 (ubiquitin-protein ligase 4 )

HSP 1 Score: 1206.0 bits (3119), Expect = 0.0e+00
Identity = 654/1297 (50.42%), Postives = 881/1297 (67.93%), Query Frame = 0

Query: 1    MAVLTFIDFFPTIIQRTALRIVVNVCKKLPSECPPNLIEAVPILCNLLQYDDEELVENVA 60
            MAVL+FIDFF T IQR A+  VVN+CK+L SE P   ++AVPILC LLQY+D +LVENVA
Sbjct: 239  MAVLSFIDFFSTSIQRVAISTVVNICKQLSSESPSPFMDAVPILCTLLQYEDRQLVENVA 298

Query: 61   RCMIKIAECVHQSSELLDGLCQHGLIQHAIRLINLNSRTTLSQTIYNDLLGVLIKLASGS 120
             C+ KIA+   +S  +LD LC+HGLI  +  L+NLNSRTTLSQ +YN ++G+L KL+SGS
Sbjct: 299  ICLTKIADQASESPAMLDQLCRHGLINESTHLLNLNSRTTLSQPVYNGVIGMLRKLSSGS 358

Query: 121  IVAFETLYELNISNTLKDILSAYNLSHGVSSSCAVVDGQRNQVCEVLKLLNELLPTEDAK 180
             +AF TLYELNI  +LK+I+S Y++SH VSS+   ++   NQV EVLKL+ ELLP    +
Sbjct: 359  ALAFRTLYELNIGYSLKEIMSTYDISHSVSST-HPINACSNQVHEVLKLVIELLPASPVE 418

Query: 181  TEQL-SEKVSFLVGNPKQLQKFGLDILPLLVQVVSSGANLYVCCGCLTIIYKFVCLGESD 240
              QL SEK SFLV  P  LQ+FG D+LP+++QV++SGAN+YV  GCL+ I+K  CL +S 
Sbjct: 419  DNQLASEKESFLVNQPDLLQQFGRDMLPVMIQVLNSGANVYVSYGCLSAIHKLTCLSKSG 478

Query: 241  MLVELLENSNISSFLAGVFTRKDHHVLMLGLKITEIILQKLASIFLKSFVKEGVYFAIDA 300
             +VELL+N+N+SS LAG+ +RKDHHV+++ L++ E++L+K    FL SF+KEGV+FAI+A
Sbjct: 479  DIVELLKNTNMSSVLAGILSRKDHHVIVVALQVAEVLLEKYRDTFLNSFIKEGVFFAIEA 538

Query: 301  LISPEKYKQLIFPVFTGVHSSFDSCQKS-SREHGKCLCYAFSSSCFPSGSETGSCKLDKD 360
            L+S ++ +Q           S D  QK  ++E  KCLC +F  S     S + +CK++KD
Sbjct: 539  LLSSDRGQQ--------NQGSADLSQKPVTKEIVKCLCQSFERSL---SSSSQTCKIEKD 598

Query: 361  SVYSLANHIRNIYFAEDLCDTDEGVTDILQNLRTFSGALDDLLNLSLIKDTPAQDEEKLY 420
            SVY LA  I+  +F  ++ ++++G+TD+LQNL+  S AL +L+ + +  D     +EK +
Sbjct: 599  SVYVLATRIKEGFFGPEVFNSEKGLTDVLQNLKNLSVALSELMTVPI--DAHVLHDEKFF 658

Query: 421  ALLAEIMSKLKCGEPISTFEFIESGIVKSFINYITNGQYLRKKEEAQTISRQFSIIERRF 480
            ++  +IM +L   E +STFEFIESG+VKS  +Y++NG Y RK  +          I +RF
Sbjct: 659  SIWNQIMERLNGRESVSTFEFIESGVVKSLASYLSNGLYQRKLSKGGPECDSLPFIGKRF 718

Query: 481  EAFARLLLSSSDHPSVNLPVLALIRKLQISLSSLENFPVISSQGFKHRNYFATVPNARCV 540
            E F RLL S  +  S       LI+KLQ SLSSLENFP++ SQ  K +N FA +PN RC 
Sbjct: 719  EVFTRLLWSDGEATS-----SLLIQKLQNSLSSLENFPIVLSQFLKQKNSFAAIPNGRCT 778

Query: 541  PHPCVKVRFVRGDGETKLCDITGDILTVDPFSSLAAIEGFLWRKVSTKKTE--QSPEETL 600
             +PC+KVRF++ +GET L D + D +TVDP   L A++ +LW KV+ +  +  ++ ++ +
Sbjct: 779  SYPCLKVRFLKAEGETSLRDYSQDFVTVDPLCYLDAVDQYLWPKVNIEPIDSVEAKDQAI 838

Query: 601  REHQIKLLSNIRSCLGVNSELVGSDIMSTDLPEV---QVPAEV---GADEKSQSSPSCSK 660
                 +L S   SC   +S  +  D  S+D  ++   QV  +    G    S S  S  K
Sbjct: 839  ECQSSQLQSTSISCQAESSSPMEIDSESSDASQLQGSQVEDQTQLPGQQNASSSETSSEK 898

Query: 661  KGTAPRLLLYLEGKQLEPTMTIYQAILQQHIKENETISGTKVWSHVYTIMYRSAGEVEDN 720
            +   PRLL  LEG +L+ ++T+YQAIL   +K     +     S  + I Y  + ++ D+
Sbjct: 899  EDAVPRLLFRLEGLELDRSLTVYQAILLHKLKSESEATNDSKLSGPHNITYERSAQLGDS 958

Query: 721  TCNQLFCASDKAPTLQFSSFFCDILDCVLPSDLA---KGS--PAYDVLFLLRSIEGMNRM 780
              N LF      P       +   L  +    LA   KGS  P YD+LFLL+S+EGMNR 
Sbjct: 959  REN-LF-----PPGSMEDDEYRPFLSYLFTHRLALRLKGSSHPPYDILFLLKSLEGMNRF 1018

Query: 781  AFHIMSHERIRAFAEGKIDTLDNIKLSVPTVSQNEFVNSKLTEKLEQQMRDSSAVSVGGM 840
             FH++S ERI AF EG+++ LD++++ V  V  +EFV+SKLTEKLEQQ+RDS AVS  G+
Sbjct: 1019 LFHLISLERINAFGEGRLENLDDLRVQVRPVPHSEFVSSKLTEKLEQQLRDSFAVSTCGL 1078

Query: 841  PLWCKELMDSCPFLFSFEARRKYFRIVVFGMPQYQLHVRSHSDLGTSNDGRSSSGGLPRK 900
            P W  +LMDSCP LFSFEA+ KYFR+  FG  + + H +  S      + R  +G LPRK
Sbjct: 1079 PPWFNDLMDSCPCLFSFEAKSKYFRLAAFGSQKIRHHPQHLSSSNVHGEARPVTGSLPRK 1138

Query: 901  KVLVHRDQILDSASKMMDQYAHQKVLLEVEYDEEVGTGLGPTLEFYTLVSREFQKYGLGM 960
            K L  R+ IL+SA+KMM+ Y +QKV++EVEY EEVGTGLGPTLEFYTLVSR FQ   LGM
Sbjct: 1139 KFLACRENILESAAKMMELYGNQKVVIEVEYSEEVGTGLGPTLEFYTLVSRAFQNPDLGM 1198

Query: 961  WRGDHDAFISGKRLNIEGRETTESPFGLFPRPWPSTLDTDKLHLPEVMKKFVLLGQIVAK 1020
            WR D  +FI GK +   G     S  GLFPRPW  T  T      +V++KFVLLG +VAK
Sbjct: 1199 WRND-CSFIVGKPVEHSG--VLASSSGLFPRPWSGTSTTS-----DVLQKFVLLGTVVAK 1258

Query: 1021 AIQDGRVLDIYFSKSFYKLILGQELSIYDIQSFDPELGTVLLEFQSLVNRNKILGTVYEE 1080
            A+QDGRVLD+  SK+FYKLILGQELS +DI   DPEL   L+E Q+LV R K+    + +
Sbjct: 1259 ALQDGRVLDLPLSKAFYKLILGQELSSFDIHFVDPELCKTLVELQALVRRKKLFAEAHGD 1318

Query: 1081 NSSSKLEFSYHNTNIEDLCLDFTLPGYPDYLLTSSQDNSMVNAKNLEDYVSLVADATLYS 1140
            + ++K + S+H T IEDLCL+F LPGY DY L     N MVN  NLE+Y+  + +AT+ +
Sbjct: 1319 SGAAKCDLSFHGTKIEDLCLEFALPGYTDYDLAPYSANDMVNLDNLEEYIKGIVNATVCN 1378

Query: 1141 GISRQIEAFKSGFNQVFPIEHLQVFTAEELERLICGEQDIWALSDLLDNMKFDHGYTSSS 1200
            GI +Q+EAF+SGFNQVF IEHL++F  EELE ++CGE D+++++++LD++KFDHGYTSSS
Sbjct: 1379 GIQKQVEAFRSGFNQVFSIEHLRIFNEEELETMLCGECDLFSMNEVLDHIKFDHGYTSSS 1438

Query: 1201 PSIIHLLEIIQEFDNKQQRAFLQFVTGAPRLPSGGFASLNPKLTIVRKHSSNLVDYDLPS 1260
            P + +LL+I+ EFD +QQRAFLQFVTG+PRLP GG ASL+PKLTIVRKH S+  D DLPS
Sbjct: 1439 PPVEYLLQILHEFDREQQRAFLQFVTGSPRLPHGGLASLSPKLTIVRKHGSDSSDTDLPS 1498

Query: 1261 VMTCANYLKLPPYSSKEIMKEKLLYAITEGQGSFHLS 1283
            VMTCANYLKLPPYSSKE MKEKL+YAITEGQGSFHLS
Sbjct: 1499 VMTCANYLKLPPYSSKEKMKEKLIYAITEGQGSFHLS 1502

BLAST of IVF0019533 vs. TAIR 10
Match: AT4G38600.1 (HEAT repeat ;HECT-domain (ubiquitin-transferase) )

HSP 1 Score: 802.7 bits (2072), Expect = 4.2e-232
Identity = 557/1574 (35.39%), Postives = 805/1574 (51.14%), Query Frame = 0

Query: 1    MAVLTFIDFFPTIIQRTALRIVVNVCKKLPSECPPNLIEAVPILCNLLQYDDEELVENVA 60
            MAVL+++DFF T +QR AL    N+CKKLPS+    ++EAVP+L NLLQY D +++E  +
Sbjct: 323  MAVLSYLDFFSTGVQRVALSTAANMCKKLPSDASDYVMEAVPLLTNLLQYHDSKVLEYAS 382

Query: 61   RCMIKIAECVHQSSELLDGLCQHGLIQHAIRLINLNS----RTTLSQTIYNDLLGVLIKL 120
             C+ +IAE      E LD LC HGL+  A  LI+ ++    + +LS + Y  L+ +L   
Sbjct: 383  ICLTRIAEAFAPYPEKLDELCNHGLVTQAASLISTSNSGGGQASLSVSTYTGLIRLLSTC 442

Query: 121  ASGSIVAFETLYELNISNTLKDILSAYNLSHGVSSSCAV---VDGQRNQVCEVLKLLNEL 180
            ASGS + F TL  L IS+ LKDIL    L  GVS++ +V   +    +Q+ E++ L NEL
Sbjct: 443  ASGSPLGFRTLLLLGISSILKDIL----LGSGVSANASVSPALSRPADQIYEIVNLANEL 502

Query: 181  L---------------------------PTEDAKTE---QLSEKVSFLVGNPKQLQKFGL 240
            L                           P+   K E   ++S +   L   P+ LQ+FGL
Sbjct: 503  LPPLPEGVISLPTSTNALVKGSCQKKSSPSTSGKQEDILKISPREKLLGDQPELLQQFGL 562

Query: 241  DILPLLVQVVSSGANLYVCCGCLTIIYKFVCLGESDMLVELLENSNISSFLAGVFTRKDH 300
            D+LP+LVQ+  S  N  +   CL++I K +    S+M+  L+ ++NISSFLAGV   KD 
Sbjct: 563  DLLPVLVQIYGSSVNGTIRHKCLSVIGKLMYFSSSEMIQSLIGDTNISSFLAGVLAWKDP 622

Query: 301  HVLMLGLKITEIILQKLASIFLKSFVKEGVYFAIDALI--------SPEKYKQLIFP--- 360
             VL+  L++ EI+++KL   F K FV+EGV  A+D L+        SP        P   
Sbjct: 623  QVLVPALQVAEILMEKLPETFSKVFVREGVVHAVDQLVLVGKPSHASPTDKDNDCVPGSA 682

Query: 361  ---VFTGVHSSFDSCQKSSREHGKCLCYAFSSSCFPSGSETGSCKLDKDSVYSLANHIRN 420
                +    S+ +S    S E          ++     + T S  L +++V S A   ++
Sbjct: 683  RSRRYRRRSSNANSDGNQSEEPKNPASLTIGANHNSLDTPTASFML-RETVSSCAKAFKD 742

Query: 421  IYFAEDLCDTDEGVTDILQNLRT----FSGALDD---------LLNLSLIKDTPAQDEEK 480
             YF  D  D D GVTD L +L+      +  +DD           +   + D  A  EE 
Sbjct: 743  KYFPSDGGDVDVGVTDDLLHLKNLCTKLTAGIDDHKVKGKGKSKASGPFLGDFSASKEEY 802

Query: 481  LYALLAEIMSKLKCGEPISTFEFIESGIVKSFINYITNGQYLRKKEEAQTISRQFSIIER 540
            L  +++EI+ ++  G+ +STFEFI SG+V + +NY + G + ++K     + +      R
Sbjct: 803  LIGVISEILGEISKGDGVSTFEFIGSGVVAALLNYFSCGYFSKEKISELNLPKLRQEGLR 862

Query: 541  RFEAFARLLLSSSDHPSVNLPVLALIRKLQISLSSLENFPVISSQGFKHRNYFATVPNA- 600
            RF+AF  + L    +     P+  LI+KLQ +LSSLE FPV+ S   +  +  A + +  
Sbjct: 863  RFKAFLEVALPFDGNEGKVPPMTVLIQKLQNALSSLERFPVVLSHPSRSLSGSARLSSGL 922

Query: 601  RCVPHPCVKVRFVRGDGETKLCDITGDILTVDPFSSLAAIEGFLWRKVS----------- 660
              + HP +K+R  R  GE  L D + +I+ +DP +SLAA+E FLW +V            
Sbjct: 923  SALAHP-LKLRLCRASGEKTLRDYSSNIVLIDPLASLAAVEEFLWPRVQRSESALKPAAP 982

Query: 661  ---------------TKKTEQSPEETLREHQIKLLSNIR--------------------- 720
                           +  +  +P  T R H  +  S I                      
Sbjct: 983  IGNTEPGTLPSGAGVSSPSSSTPASTTRRHSSRSRSAINIGDTSKKDPVHEKGTSSSKGK 1042

Query: 721  ------------------------------------------------SCLGVNSELV-- 780
                                                            S + ++  LV  
Sbjct: 1043 GKGVMKPAQADKGPQTRSNAQKRAVLDKDTQMKPASGDSSSEDEELEISPVDIDDALVIE 1102

Query: 781  -----------GSDIMSTDLP--------EVQVPAEVGAD-----EKSQSSPSCSKKGTA 840
                         D++   LP        +V++   V  D      +  +  S    G A
Sbjct: 1103 EDDISDDEDDDNEDVLDDSLPMCTPDKVHDVKLADSVDDDGLATSGRQMNPASGGTSGAA 1162

Query: 841  ----------------------------------------------------------PR 900
                                                                       +
Sbjct: 1163 AARASDSIDTGIGNSYGSRGALSFAAAAMAGLGAASGRGIRGSRDLHGRTLNRSSDEPSK 1222

Query: 901  LLLYLEGKQLEPTMTIYQAILQQHI---KENETISGTKV-------WSHVYTIMYR---- 960
            L+    GKQL   +TIYQA+ +Q +    +++   G+ +       ++ +YTIMY+    
Sbjct: 1223 LIFTAAGKQLSRHLTIYQAVQRQLMLDEDDDDRFGGSDLVSSDGSRFNDIYTIMYQRPDS 1282

Query: 961  -----SAGEVEDNTCNQLFCASDKAPTLQFSSFFCDILDCV----LPSDLAKGSPAYDVL 1020
                 S G     T ++   ++    +++  S    +LD +    LP DL K +  Y+VL
Sbjct: 1283 QVNRLSVGGASSTTPSKSTKSATTNSSVESQSHRASLLDSILQGELPCDLEKSNSTYNVL 1342

Query: 1021 FLLRSIEGMNRMAFHIMSHERIRAFAEGKIDTLDNIKLSVPTVSQNEFVNSKLTEKLEQQ 1080
             LLR +EG+N++   + +      FAEGKI +LD++  +   V  +EFVNSKLT KL +Q
Sbjct: 1343 ALLRVLEGLNQLCPRLRAQTLSDRFAEGKITSLDDLSTTAAKVPLDEFVNSKLTPKLARQ 1402

Query: 1081 MRDSSAVSVGGMPLWCKELMDSCPFLFSFEARRKYFRIVVFGMPQ--YQLHVRSHSD-LG 1140
            ++D+ A+  G +P WC +L  +CPFLF F+ RR+YF    FG+ +   +L  +  +D  G
Sbjct: 1403 IQDALALCSGSLPSWCYQLTRACPFLFPFQTRRQYFYSTAFGLSRALNRLQQQQGADGSG 1462

Query: 1141 TSNDGRSSSGGLPRKKVLVHRDQILDSASKMMDQYAHQKVLLEVEYDEEVGTGLGPTLEF 1200
            ++N+     G L R+KV V R++ILDSA+K+M+ Y+ QK +LEVEY  EVGTGLGPTLEF
Sbjct: 1463 STNEREMRIGRLQRQKVRVSRNRILDSAAKVMEMYSSQKAVLEVEYFGEVGTGLGPTLEF 1522

Query: 1201 YTLVSREFQKYGLGMWR---GDHDAFISGK------RLNIEGRETTESPFGLFPRPWPST 1260
            YTL+S + QK  LGMWR   GD  +   G+      + +   R+   +P GLFPRPWPST
Sbjct: 1523 YTLLSHDLQKASLGMWRSSSGDKVSMQIGRDEIEDGKPSAANRDIVLAPLGLFPRPWPST 1582

Query: 1261 LD-TDKLHLPEVMKKFVLLGQIVAKAIQDGRVLDIYFSKSFYKLILGQELSIYDIQSFDP 1283
             D ++     +V++ F LLG+++AKA+QDGR+LD+  S +FYKLILGQEL ++DI  FD 
Sbjct: 1583 ADISEGGQFHKVIEYFRLLGRVMAKALQDGRLLDVPLSTAFYKLILGQELDLHDIVLFDA 1642

BLAST of IVF0019533 vs. TAIR 10
Match: AT4G38600.2 (HEAT repeat ;HECT-domain (ubiquitin-transferase) )

HSP 1 Score: 800.4 bits (2066), Expect = 2.1e-231
Identity = 556/1560 (35.64%), Postives = 804/1560 (51.54%), Query Frame = 0

Query: 1    MAVLTFIDFFPTIIQRTALRIVVNVCKKLPSECPPNLIEAVPILCNLLQYDDEELVENVA 60
            MAVL+++DFF T +QR AL    N+CKKLPS+    ++EAVP+L NLLQY D +++E  +
Sbjct: 250  MAVLSYLDFFSTGVQRVALSTAANMCKKLPSDASDYVMEAVPLLTNLLQYHDSKVLEYAS 309

Query: 61   RCMIKIAECVHQSSELLDGLCQHGLIQHAIRLINLNS----RTTLSQTIYNDLLGVLIKL 120
             C+ +IAE      E LD LC HGL+  A  LI+ ++    + +LS + Y  L+ +L   
Sbjct: 310  ICLTRIAEAFAPYPEKLDELCNHGLVTQAASLISTSNSGGGQASLSVSTYTGLIRLLSTC 369

Query: 121  ASGSIVAFETLYELNISNTLKDILSAYNLSHGVSSSCAV---VDGQRNQVCEVLKLLNEL 180
            ASGS + F TL  L IS+ LKDIL    L  GVS++ +V   +    +Q+ E++ L NEL
Sbjct: 370  ASGSPLGFRTLLLLGISSILKDIL----LGSGVSANASVSPALSRPADQIYEIVNLANEL 429

Query: 181  L---------------------------PTEDAKTE---QLSEKVSFLVGNPKQLQKFGL 240
            L                           P+   K E   ++S +   L   P+ LQ+FGL
Sbjct: 430  LPPLPEGVISLPTSTNALVKGSCQKKSSPSTSGKQEDILKISPREKLLGDQPELLQQFGL 489

Query: 241  DILPLLVQVVSSGANLYVCCGCLTIIYKFVCLGESDMLVELLENSNISSFLAGVFTRKDH 300
            D+LP+LVQ+  S  N  +   CL++I K +    S+M+  L+ ++NISSFLAGV   KD 
Sbjct: 490  DLLPVLVQIYGSSVNGTIRHKCLSVIGKLMYFSSSEMIQSLIGDTNISSFLAGVLAWKDP 549

Query: 301  HVLMLGLKITEIILQKLASIFLKSFVKEGVYFAIDALI--------SPEKYKQLIFP--- 360
             VL+  L++ EI+++KL   F K FV+EGV  A+D L+        SP        P   
Sbjct: 550  QVLVPALQVAEILMEKLPETFSKVFVREGVVHAVDQLVLVGKPSHASPTDKDNDCVPGSA 609

Query: 361  ---VFTGVHSSFDSCQKSSREHGKCLCYAFSSSCFPSGSETGSCKLDKDSVYSLANHIRN 420
                +    S+ +S    S E          ++     + T S  L +++V S A   ++
Sbjct: 610  RSRRYRRRSSNANSDGNQSEEPKNPASLTIGANHNSLDTPTASFML-RETVSSCAKAFKD 669

Query: 421  IYFAEDLCDTDEGVTDILQNLRT----FSGALDD---------LLNLSLIKDTPAQDEEK 480
             YF  D  D D GVTD L +L+      +  +DD           +   + D  A  EE 
Sbjct: 670  KYFPSDGGDVDVGVTDDLLHLKNLCTKLTAGIDDHKVKGKGKSKASGPFLGDFSASKEEY 729

Query: 481  LYALLAEIMSKLKCGEPISTFEFIESGIVKSFINYITNGQYLRKKEEAQTISRQFSIIER 540
            L  +++EI+ ++  G+ +STFEFI SG+V + +NY + G + ++K     + +      R
Sbjct: 730  LIGVISEILGEISKGDGVSTFEFIGSGVVAALLNYFSCGYFSKEKISELNLPKLRQEGLR 789

Query: 541  RFEAFARLLLSSSDHPSVNLPVLALIRKLQISLSSLENFPVISSQGFKHRNYFATVPNA- 600
            RF+AF  + L    +     P+  LI+KLQ +LSSLE FPV+ S   +  +  A + +  
Sbjct: 790  RFKAFLEVALPFDGNEGKVPPMTVLIQKLQNALSSLERFPVVLSHPSRSLSGSARLSSGL 849

Query: 601  RCVPHPCVKVRFVRGDGETKLCDITGDILTVDPFSSLAAIEGFLWRKVS----------- 660
              + HP +K+R  R  GE  L D + +I+ +DP +SLAA+E FLW +V            
Sbjct: 850  SALAHP-LKLRLCRASGEKTLRDYSSNIVLIDPLASLAAVEEFLWPRVQRSESALKPAAP 909

Query: 661  ---------------TKKTEQSPEETLREHQIKLLSNIRSCLGV---------------N 720
                           +  +  +P  T R H     S  RS + +               +
Sbjct: 910  IGNTEPGTLPSGAGVSSPSSSTPASTTRRHS----SRSRSAINIGDTSKKDPVHEKGTSS 969

Query: 721  SELVGSDIM----STDLPEVQVPAEVGADEKSQSSP------------------------ 780
            S+  G  +M    +   P+ +  A+  ADE+ + SP                        
Sbjct: 970  SKGKGKGVMKPAQADKGPQTRSNAQKRADEELEISPVDIDDALVIEEDDISDDEDDDNED 1029

Query: 781  --------------------------------------SCSKKGTA-------------- 840
                                                  S    G A              
Sbjct: 1030 VLDDSLPMCTPDKVHDVKLADSVDDDGLATSGRQMNPASGGTSGAAAARASDSIDTGIGN 1089

Query: 841  --------------------------------------------PRLLLYLEGKQLEPTM 900
                                                         +L+    GKQL   +
Sbjct: 1090 SYGSRGALSFAAAAMAGLGAASGRGIRGSRDLHGRTLNRSSDEPSKLIFTAAGKQLSRHL 1149

Query: 901  TIYQAILQQHI---KENETISGTKV-------WSHVYTIMYR---------SAGEVEDNT 960
            TIYQA+ +Q +    +++   G+ +       ++ +YTIMY+         S G     T
Sbjct: 1150 TIYQAVQRQLMLDEDDDDRFGGSDLVSSDGSRFNDIYTIMYQRPDSQVNRLSVGGASSTT 1209

Query: 961  CNQLFCASDKAPTLQFSSFFCDILDCV----LPSDLAKGSPAYDVLFLLRSIEGMNRMAF 1020
             ++   ++    +++  S    +LD +    LP DL K +  Y+VL LLR +EG+N++  
Sbjct: 1210 PSKSTKSATTNSSVESQSHRASLLDSILQGELPCDLEKSNSTYNVLALLRVLEGLNQLCP 1269

Query: 1021 HIMSHERIRAFAEGKIDTLDNIKLSVPTVSQNEFVNSKLTEKLEQQMRDSSAVSVGGMPL 1080
             + +      FAEGKI +LD++  +   V  +EFVNSKLT KL +Q++D+ A+  G +P 
Sbjct: 1270 RLRAQTLSDRFAEGKITSLDDLSTTAAKVPLDEFVNSKLTPKLARQIQDALALCSGSLPS 1329

Query: 1081 WCKELMDSCPFLFSFEARRKYFRIVVFGMPQ--YQLHVRSHSD-LGTSNDGRSSSGGLPR 1140
            WC +L  +CPFLF F+ RR+YF    FG+ +   +L  +  +D  G++N+     G L R
Sbjct: 1330 WCYQLTRACPFLFPFQTRRQYFYSTAFGLSRALNRLQQQQGADGSGSTNEREMRIGRLQR 1389

Query: 1141 KKVLVHRDQILDSASKMMDQYAHQKVLLEVEYDEEVGTGLGPTLEFYTLVSREFQKYGLG 1200
            +KV V R++ILDSA+K+M+ Y+ QK +LEVEY  EVGTGLGPTLEFYTL+S + QK  LG
Sbjct: 1390 QKVRVSRNRILDSAAKVMEMYSSQKAVLEVEYFGEVGTGLGPTLEFYTLLSHDLQKASLG 1449

Query: 1201 MWR---GDHDAFISGK------RLNIEGRETTESPFGLFPRPWPSTLD-TDKLHLPEVMK 1260
            MWR   GD  +   G+      + +   R+   +P GLFPRPWPST D ++     +V++
Sbjct: 1450 MWRSSSGDKVSMQIGRDEIEDGKPSAANRDIVLAPLGLFPRPWPSTADISEGGQFHKVIE 1509

Query: 1261 KFVLLGQIVAKAIQDGRVLDIYFSKSFYKLILGQELSIYDIQSFDPELGTVLLEFQSLVN 1283
             F LLG+++AKA+QDGR+LD+  S +FYKLILGQEL ++DI  FD ELG  L E + +V 
Sbjct: 1510 YFRLLGRVMAKALQDGRLLDVPLSTAFYKLILGQELDLHDIVLFDAELGKTLQELRVVVA 1569

BLAST of IVF0019533 vs. TAIR 10
Match: AT1G70320.1 (ubiquitin-protein ligase 2 )

HSP 1 Score: 155.2 bits (391), Expect = 3.5e-37
Identity = 104/300 (34.67%), Postives = 155/300 (51.67%), Query Frame = 0

Query: 985  DKLHLPEVMKKFVLLGQIVAKAIQDGRVLDIYFSKSFYKLILGQELSIYDIQSFDPELGT 1044
            + ++  E +  F  +G++VAKA+ DG++LD+YF++SFYK ILG +++ +DI++ DP+   
Sbjct: 3374 NSVYQTEHLSYFKFVGRMVAKALFDGQLLDVYFTRSFYKHILGVKVTYHDIEAVDPDY-- 3433

Query: 1045 VLLEFQSLVNRNKILGTVYEENSSSKLEFSYHNTNIEDLCLDFTLPGYPDYLLTSSQDNS 1104
                        K L  + E + S  L+ ++     E+  + +      DY L     N 
Sbjct: 3434 -----------YKNLKWLLENDVSDILDLTFSMDADEEKHILYEKTEVTDYELKPGGRNI 3493

Query: 1105 MVNAKNLEDYVSLVADATLYSGISRQIEAFKSGFNQVFPIEHLQVFTAEELERLICGEQD 1164
             V  +   +YV LVAD  L S I  QI AF  G N++ P E + +F  +ELE LI G  +
Sbjct: 3494 RVTEETKHEYVDLVADHILTSAIRPQINAFLEGLNELIPRELVSIFNDKELELLISGLPE 3553

Query: 1165 IWALSDLLDNMKFDHGYTSSSPSIIHLLEIIQEFDNKQQRAFLQFVTGAPRLPSGGFASL 1224
            I    DL  N ++   YT  SP I    E+++ F  +    FLQFVTG  ++P  GF +L
Sbjct: 3554 I-DFDDLKANTEYT-SYTVGSPVIRWFWEVVKAFSKEDMARFLQFVTGTSKVPLEGFKAL 3613

Query: 1225 N-----PKLTIVRKHSSNLVDYDLPSVMTCANYLKLPPYSSKEIMKEKLLYAITEGQGSF 1280
                   +L I + + S      LPS  TC N L LP Y SKE ++E+LL AI E    F
Sbjct: 3614 QGISGPQRLQIHKAYGS---PERLPSAHTCFNQLDLPEYQSKEQVQERLLLAIHEANEGF 3655

BLAST of IVF0019533 vs. TAIR 10
Match: AT1G55860.1 (ubiquitin-protein ligase 1 )

HSP 1 Score: 154.5 bits (389), Expect = 5.9e-37
Identity = 103/300 (34.33%), Postives = 155/300 (51.67%), Query Frame = 0

Query: 985  DKLHLPEVMKKFVLLGQIVAKAIQDGRVLDIYFSKSFYKLILGQELSIYDIQSFDPELGT 1044
            + ++  E +  F  +G++VAKA+ DG++LD+YF++SFYK ILG +++ +DI++ DP+   
Sbjct: 3646 NSVYQTEHLSYFKFVGRMVAKALFDGQLLDVYFTRSFYKHILGVKVTYHDIEAVDPDY-- 3705

Query: 1045 VLLEFQSLVNRNKILGTVYEENSSSKLEFSYHNTNIEDLCLDFTLPGYPDYLLTSSQDNS 1104
                        K L  + E + S  L+ ++     E+  + +      DY L     N 
Sbjct: 3706 -----------YKNLKWLLENDVSDILDLTFSMDADEEKHILYEKTEVTDYELKPGGRNI 3765

Query: 1105 MVNAKNLEDYVSLVADATLYSGISRQIEAFKSGFNQVFPIEHLQVFTAEELERLICGEQD 1164
             V  +   +YV LVA   L + I  QI AF  GFN++ P E + +F  +ELE LI G  +
Sbjct: 3766 RVTEETKHEYVDLVAGHILTNAIRPQINAFLEGFNELIPRELVSIFNDKELELLISGLPE 3825

Query: 1165 IWALSDLLDNMKFDHGYTSSSPSIIHLLEIIQEFDNKQQRAFLQFVTGAPRLPSGGFASL 1224
            I    DL  N ++   YT+ SP I    E+++ F  +    FLQFVTG  ++P  GF +L
Sbjct: 3826 I-DFDDLKANTEYT-SYTAGSPVIHWFWEVVKAFSKEDMARFLQFVTGTSKVPLEGFKAL 3885

Query: 1225 N-----PKLTIVRKHSSNLVDYDLPSVMTCANYLKLPPYSSKEIMKEKLLYAITEGQGSF 1280
                   +L I   H +      LPS  TC N L LP Y SKE ++E+LL AI E    F
Sbjct: 3886 QGISGPQRLQI---HKAYGAPERLPSAHTCFNQLDLPEYQSKEQLQERLLLAIHEASEGF 3927

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LYZ70.0e+0050.42E3 ubiquitin-protein ligase UPL4 OS=Arabidopsis thaliana OX=3702 GN=UPL4 PE=3 SV... [more]
Q6WWW45.9e-23135.39E3 ubiquitin-protein ligase UPL3 OS=Arabidopsis thaliana OX=3702 GN=UPL3 PE=1 SV... [more]
F1RCR61.0e-12126.94E3 ubiquitin-protein ligase TRIP12 OS=Danio rerio OX=7955 GN=trip12 PE=3 SV=1[more]
Q146691.9e-12026.54E3 ubiquitin-protein ligase TRIP12 OS=Homo sapiens OX=9606 GN=TRIP12 PE=1 SV=1[more]
E1B7Q77.1e-12026.47E3 ubiquitin-protein ligase TRIP12 OS=Bos taurus OX=9913 GN=TRIP12 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
A0A1S3B6650.0e+00100.00E3 ubiquitin-protein ligase UPL4 OS=Cucumis melo OX=3656 GN=LOC103486449 PE=4 SV... [more]
A0A5D3DN910.0e+0099.92E3 ubiquitin-protein ligase UPL4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A5A7TQD30.0e+0098.01E3 ubiquitin-protein ligase UPL4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27... [more]
A0A0A0LDW70.0e+0094.93HECT domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G736800 PE=4 S... [more]
A0A6J1J7K50.0e+0086.67E3 ubiquitin-protein ligase UPL4-like isoform X1 OS=Cucurbita maxima OX=3661 GN=... [more]
Match NameE-valueIdentityDescription
XP_008442639.10.0100.00PREDICTED: E3 ubiquitin-protein ligase UPL4 [Cucumis melo][more]
TYK25103.10.099.92E3 ubiquitin-protein ligase UPL4 [Cucumis melo var. makuwa][more]
KAA0044037.10.098.01E3 ubiquitin-protein ligase UPL4 [Cucumis melo var. makuwa][more]
XP_004137960.20.094.93E3 ubiquitin-protein ligase UPL4 [Cucumis sativus] >KGN58932.1 hypothetical prot... [more]
XP_038904018.10.091.43E3 ubiquitin-protein ligase UPL4 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT5G02880.10.0e+0050.42ubiquitin-protein ligase 4 [more]
AT4G38600.14.2e-23235.39HEAT repeat ;HECT-domain (ubiquitin-transferase) [more]
AT4G38600.22.1e-23135.64HEAT repeat ;HECT-domain (ubiquitin-transferase) [more]
AT1G70320.13.5e-3734.67ubiquitin-protein ligase 2 [more]
AT1G55860.15.9e-3734.33ubiquitin-protein ligase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000569HECT domainSMARTSM00119hect_3coord: 895..1282
e-value: 3.9E-96
score: 335.4
IPR000569HECT domainPFAMPF00632HECTcoord: 933..1282
e-value: 2.2E-83
score: 280.3
IPR000569HECT domainPROSITEPS50237HECTcoord: 902..1282
score: 63.186672
IPR000569HECT domainCDDcd00078HECTccoord: 886..1280
e-value: 2.48377E-113
score: 356.875
IPR011989Armadillo-like helicalGENE3D1.25.10.10coord: 1..307
e-value: 6.3E-21
score: 76.6
NoneNo IPR availableGENE3D3.30.2410.10Hect, E3 ligase catalytic domaincoord: 1166..1280
e-value: 4.6E-34
score: 119.1
NoneNo IPR availableGENE3D3.90.1750.10Hect, E3 ligase catalytic domainscoord: 829..1058
e-value: 1.4E-38
score: 134.3
NoneNo IPR availablePANTHERPTHR45670E3 UBIQUITIN-PROTEIN LIGASE TRIP12coord: 1..1282
NoneNo IPR availablePANTHERPTHR45670:SF10E3 UBIQUITIN-PROTEIN LIGASE UPL4coord: 1..1282
IPR016024Armadillo-type foldSUPERFAMILY48371ARM repeatcoord: 8..287
IPR016024Armadillo-type foldSUPERFAMILY48371ARM repeatcoord: 192..503
IPR035983HECT, E3 ligase catalytic domainSUPERFAMILY56204Hect, E3 ligase catalytic domaincoord: 882..1275

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
IVF0019533.1IVF0019533.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
molecular_function GO:0004842 ubiquitin-protein transferase activity