IVF0018661 (gene) Melon (IVF77) v1

Overview
NameIVF0018661
Typegene
OrganismCucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
DescriptionAlpha-amylase
Locationchr07: 500298 .. 504531 (-)
RNA-Seq ExpressionIVF0018661
SyntenyIVF0018661
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGTGACCATGATAATGTATGCTTCTTGTTTAATTTGGAAATTGTTTCCTTGATGAGTTTTCTGCATCAGTGTGATATGCTTTTAGTTATAAATTTGTCGGAATTAGAATATAGATTCTGATATACAAATTACTTATTTATGTTGGAATTCAGGTTTTCGATGAAAACTACGAACAGACTGATACTGGTATGGTTTTTTCTTTGTAAATTTCACATCATGGTATGCTCACGTCGATCTTAAGTGTTTGTCAAAAGATCATACCTCTCTTGGAAGTGACTTCAAACTCTAATACATTTGCTTGGTCTAACTAACTAAAGAGAATGTTTGATGATGCTCAAAATTTGACTCAGGGATAAGAAATTTGAAAAGAAATATATGCTTCTTAGGTGTGAGTACGTTGCAATATTTGCTTTTAGTTTACATAAAATTTGTGGTTTATGAAAAGGATTGAAACACGTGGATTTGAAATCAGAGCTGGATTTCTGCTACGTAAATTTTCTTTTGTTTGAAATTATTTTATCGTTTTAATAATTTCAGTGTTTCTTTATGGTCACCTGTAGGGGCAGTGATACGCAATGGAAGAGAAATCCTTTTCCAGGTAGAGGTTACAATTTCAGCTCTCTGATTTTTCTCTAGAGCAGAGGTCAGCAGGAAGGAAAATATTATCTTGCTCTTAATTGAGCCGAAGTCTCGGAAACAGAAAAAGAAAAAAATGTTTGCCGTTTGAAATTCATGCTCGATGTTGTTGATTCTTTTAATGTAAATCGGCTAGAATTTGAGGTGGAAGGACTTCGGGACTGTCAATGTTTTGGATCAAATCTCCAGGTCATGCTAAACTTATGGGAGAAATTTTGCACCCATAATTTCTATTGTAATTACAGGCATTCAACTGGGAATCTCATAAATGTGATTGGTCGGGAAATCTAGAGTGTAAAGTTCATGATATCGCAAAAAGTGGATTTACCTCTGCATGGTTGCCTCCTCCAACTCATTCCATTGCTCCTCAAGGTCAAAGGAACAAATTCACTTGAATTTTTATGTTCCTTTCACATATATGCGTGCATAACTCAATATTTTCTTCACAATTTCAGGTTACCTCCCTCAGAACCTTTACTCCCTAAACTCTTCATACGGTTCTGAGGACATGCTGAAAGCTTTACTTCTCAAAATGAAGCAGTGCAAGGTTAGAGCAATGGCTGACATAGTCATCAATCATCGTGTTGGAACTGTTGTAGGACATGGAGGAATGCATAATCGCTTTGATGGAATACTATTACCATGGGATGAGCATGCTGTTACCTCGTGTTCTGGTGGCCTGGTATTTTTCTGTTACAAATTCCAAGTGAGCTCATAATTATAAGAAAACCTTTGGTCTTTAGTCTGTGGTATCTGTTCTAGGGCAATCGTAGCACTGGTGACAACTTCCATGGGGTTCCAAACATTGATCATACCCAACCTTTTGTCCGTAGAGATATCATAGCATGGCTGAAGTGGCTACGCAGTAGTGTTGGATTTCAGGATTTTCGTTTTGATTTTGCAAGGGGGTAATATTGTCCTTCTCTGTTTGTGTGTGTGTGTAAATATATTGTGGGGAGGCTGGGGCGTTACAGAGGTTCAAGGATAAATTGTTCTGAAAATGACAAGATCCCTCCTCTGTTTCAAAATTTCACTGTATCATCTTCTCAACATTCGGCTTGTCTTTTCTTGTAAGGAGTTCTTCAATGGATTATAACATACATTTGCCGTCTGCTTTTTTTTTTTTTTTTGATGCTTTAACTCAGTTTTTCTGCAAAATTCGTGAAAGAATATATTGAAGCAGCAAAGCCAATGTTTTCTATTGGGGAATATTGGGATTCTTGTAATTATAATGGCCATGACTTGGACTATAATCAAGGTAACATGATGATGCATTATGTTGATAAATTGAAATCATGTTTTCAAGTTTCAGGATTTTTAGTTTTTTTGAATGGTCTTGTCTGTGAATAATATCCTTTGTTCGTCTTATCAATTCATGTGTTCCATAGTGATGATTACAGCTCTAAATGCAGATGGACATAGACAGCGGATAATAAATTGGATTGATGGCACAGGACAACTTTCAGCTGCTTTTGATTTTACAACTAAGGGAGTCCTTCAGGTATTGGTGTTTTGTGAGGCCATTGCTAACTTCCTTCCCTCCTAAAAATTGTAATACTACGGAGCTCAAATCTTAATTACTTAAAATAGAAAGGATGAAGTGACAGCTATGTCATGGGTTTTTTCCCCTCTAGATCAGTAGATCCACAACATTTGACATTTTACAACTCTTTCTATTTGCTTCTTTTCTCCTTCTTTATTAAGTGAAGTTTGGGTTTTGTTAATATTTGCTCTTGCTAGTACATTGCTTGACTAACATGCATTTGTTTCCTTGTTTGCTTAGTAATAAACATAGTATAATTAATTTTGCTTGTAGGAAGCTGTTAAAGGACAATTATGGCGTCTGTGTGACTGTCGAGGAAAGCCACCCGGTGTGATGGGATGGTGGCCTTCAAGGGCCGTGACATTCTTAGATAACCATGATACTGGTTCAACACAGGTACCATGTCTTCAAATCTGTTCGTGAAATTATTGGTTGACAAATTTCAACATTTGTTTTCATTGCTCATTGTGCATGTGCCTTTTAAAATTGTCTCAACTTGACCGCGTATCCATGTCCTTTTATTTGGATTAATATTTGACAACACGTATTTGGAAGTCATGCCCTGTATTGGGGATTGAGTGTGACCAGACCATACATGAAAATGTCATAGGAAGCAAGGGAGAAAGGAAGTATTGATGCATACCTGAATGAAGTTGTAATGTCTGGTTGTTCTACACGGCTTCTATATGGCCAAATATGATGCCATCAGAACAGCTACTGGACTCTGTTCCATTTTTCTATTTTTCTATTATCTTTTGTAAGATTCGTATGTGTTTTTTGCAACATGTAACTGAGATTTATTGGCCAAGCTTTCTTTCATATAGGTTTGTTCAAATTGAACTAATTAAAATGTAAATAGTTCTAGGCTTCATCCAACTCTGCATCTTATTGAGTAGATAGCTTTAGATTATTATTCGGAAACTCTTAATTAAATTTTTTTCCTAGGTTGAAAGCAAGCATCAAGTGTGCTTTCAAGTATAGATATTGACTTGAGCAGATCATGACAATTAATGCAACTCGACATGCTTGGCAAATGAGCTATATAATTCTTCATATGTATTTTCTTGAAACATTTATAGAAGGTGAAATTGTACCATTAGAATGTTGGTGACTCCACTAACCTCAGCGTACCATTGTTCTTGCAGGCCCATTGGCCCTTTCCTGCCAACCATATCATGGAGGTATGCAAGTGTGGCATATCATTCCATTTGCAATCTCTTTATTGCTTATAGTCTTATTCTTGCTGCTGTGGCTTCATCGTGGTTTTTGGAAGCTCTCAATTGTTTGGTCCAATTATTCGTTGTGTAAAGTCTAATTATCCCCCATCAGGTGGTCCTCATTGTTCCCTGTGTGTTTTGGAAGCTTTCAATTGTTTGTTCCAATTGATCCTATGTATGACTGTCTATACATTTGAAGTTAGTTTCCTACTTTTAGCTGCAAATTCTATGGCTATAACTGTTCAGTATAAATTTAAAAAAAGAAAAAAGAAAAAAAAAAAAAAACTGTTCAGCATACTAAGCAATCAATCTTTGATGTTTCTGGGTGAATTTCGTTGAAAGAGCTTTATAATTACTTTTATGCTTGGCCTAGAGTCGTCACTTGTCTTCCAGTAAAAAAAATTCCGTCATGTTTCTATTTTCTGATTCCTGGAGCTTTGGTTATTCCCTGCAGGGCTATGCATATATTCTCACACACCCAGGGATACCAACAGTATTTTATGACCACTTTTACGATTGGGGTGACTCAGTGCACGACGAGATAGTAAAATTAGTATGTGGCATAGTCAATAATGCTTCAATAGAGTTTGTTATCTTCTATTCAAGCAAACTTCTCATAGTACTTAAAATTTTATTTTTCTTGCAGATAGACATTCGAAAACATCAAGACATCAACAGTCGGTCATCTGTGAAGATCCTCGAGGCACGATCAGATCTTTATTCTGCAATAATTGGGGAGAACTTGTGCATGAAAATCGGAGATGGTTCTTGGTGTCCTGGTACGGAATGGACTCTTGCAACCTATGGATGCAGATATGCAGTGTGGCAGAAATAA

mRNA sequence

ATGGGTGACCATGATAATGTTTTCGATGAAAACTACGAACAGACTGATACTGGGGCAGTGATACGCAATGGAAGAGAAATCCTTTTCCAGGCATTCAACTGGGAATCTCATAAATGTGATTGGTCGGGAAATCTAGAGTGTAAAGTTCATGATATCGCAAAAAGTGGATTTACCTCTGCATGGTTGCCTCCTCCAACTCATTCCATTGCTCCTCAAGGTTACCTCCCTCAGAACCTTTACTCCCTAAACTCTTCATACGGTTCTGAGGACATGCTGAAAGCTTTACTTCTCAAAATGAAGCAGTGCAAGGTTAGAGCAATGGCTGACATAGTCATCAATCATCGTGTTGGAACTGTTGTAGGACATGGAGGAATGCATAATCGCTTTGATGGAATACTATTACCATGGGATGAGCATGCTGTTACCTCGTGTTCTGGTGGCCTGGGCAATCGTAGCACTGGTGACAACTTCCATGGGGTTCCAAACATTGATCATACCCAACCTTTTGTCCGTAGAGATATCATAGCATGGCTGAAGTGGCTACGCAGTAGTGTTGGATTTCAGGATTTTCGTTTTGATTTTGCAAGGGGTTTTTCTGCAAAATTCGTGAAAGAATATATTGAAGCAGCAAAGCCAATGTTTTCTATTGGGGAATATTGGGATTCTTGTAATTATAATGGCCATGACTTGGACTATAATCAAGATGGACATAGACAGCGGATAATAAATTGGATTGATGGCACAGGACAACTTTCAGCTGCTTTTGATTTTACAACTAAGGGAGTCCTTCAGGAAGCTGTTAAAGGACAATTATGGCGTCTGTGTGACTGTCGAGGAAAGCCACCCGGTGTGATGGGATGGTGGCCTTCAAGGGCCGTGACATTCTTAGATAACCATGATACTGGTTCAACACAGGCCCATTGGCCCTTTCCTGCCAACCATATCATGGAGGGCTATGCATATATTCTCACACACCCAGGGATACCAACAGTATTTTATGACCACTTTTACGATTGGGGTGACTCAGTGCACGACGAGATAGTAAAATTAATAGACATTCGAAAACATCAAGACATCAACAGTCGGTCATCTGTGAAGATCCTCGAGGCACGATCAGATCTTTATTCTGCAATAATTGGGGAGAACTTGTGCATGAAAATCGGAGATGGTTCTTGGTGTCCTGGTACGGAATGGACTCTTGCAACCTATGGATGCAGATATGCAGTGTGGCAGAAATAA

Coding sequence (CDS)

ATGGGTGACCATGATAATGTTTTCGATGAAAACTACGAACAGACTGATACTGGGGCAGTGATACGCAATGGAAGAGAAATCCTTTTCCAGGCATTCAACTGGGAATCTCATAAATGTGATTGGTCGGGAAATCTAGAGTGTAAAGTTCATGATATCGCAAAAAGTGGATTTACCTCTGCATGGTTGCCTCCTCCAACTCATTCCATTGCTCCTCAAGGTTACCTCCCTCAGAACCTTTACTCCCTAAACTCTTCATACGGTTCTGAGGACATGCTGAAAGCTTTACTTCTCAAAATGAAGCAGTGCAAGGTTAGAGCAATGGCTGACATAGTCATCAATCATCGTGTTGGAACTGTTGTAGGACATGGAGGAATGCATAATCGCTTTGATGGAATACTATTACCATGGGATGAGCATGCTGTTACCTCGTGTTCTGGTGGCCTGGGCAATCGTAGCACTGGTGACAACTTCCATGGGGTTCCAAACATTGATCATACCCAACCTTTTGTCCGTAGAGATATCATAGCATGGCTGAAGTGGCTACGCAGTAGTGTTGGATTTCAGGATTTTCGTTTTGATTTTGCAAGGGGTTTTTCTGCAAAATTCGTGAAAGAATATATTGAAGCAGCAAAGCCAATGTTTTCTATTGGGGAATATTGGGATTCTTGTAATTATAATGGCCATGACTTGGACTATAATCAAGATGGACATAGACAGCGGATAATAAATTGGATTGATGGCACAGGACAACTTTCAGCTGCTTTTGATTTTACAACTAAGGGAGTCCTTCAGGAAGCTGTTAAAGGACAATTATGGCGTCTGTGTGACTGTCGAGGAAAGCCACCCGGTGTGATGGGATGGTGGCCTTCAAGGGCCGTGACATTCTTAGATAACCATGATACTGGTTCAACACAGGCCCATTGGCCCTTTCCTGCCAACCATATCATGGAGGGCTATGCATATATTCTCACACACCCAGGGATACCAACAGTATTTTATGACCACTTTTACGATTGGGGTGACTCAGTGCACGACGAGATAGTAAAATTAATAGACATTCGAAAACATCAAGACATCAACAGTCGGTCATCTGTGAAGATCCTCGAGGCACGATCAGATCTTTATTCTGCAATAATTGGGGAGAACTTGTGCATGAAAATCGGAGATGGTTCTTGGTGTCCTGGTACGGAATGGACTCTTGCAACCTATGGATGCAGATATGCAGTGTGGCAGAAATAA

Protein sequence

MGDHDNVFDENYEQTDTGAVIRNGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSAWLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVVGHGGMHNRFDGILLPWDEHAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKWLRSSVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQDGHRQRIINWIDGTGQLSAAFDFTTKGVLQEAVKGQLWRLCDCRGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIDIRKHQDINSRSSVKILEARSDLYSAIIGENLCMKIGDGSWCPGTEWTLATYGCRYAVWQK
Homology
BLAST of IVF0018661 vs. ExPASy Swiss-Prot
Match: Q8LFG1 (Probable alpha-amylase 2 OS=Arabidopsis thaliana OX=3702 GN=AMY2 PE=2 SV=1)

HSP 1 Score: 731.1 bits (1886), Expect = 7.0e-210
Identity = 326/413 (78.93%), Postives = 375/413 (90.80%), Query Frame = 0

Query: 1   MGDHDNVFDENYEQTDTGAVIRNGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSA 60
           MG ++NVFDE  +QTD G VIR+GRE++ QA+NWESHK DW  NL+ KV DIAKSGFTSA
Sbjct: 1   MGYYNNVFDECNDQTDIGRVIRDGREVILQAYNWESHKYDWWRNLDGKVPDIAKSGFTSA 60

Query: 61  WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVV 120
           WLPPP+ S+AP+GYLPQ+LYSLNS+YGSE +LK+LL KMKQ KVRAMADIVINHRVGT  
Sbjct: 61  WLPPPSQSLAPEGYLPQDLYSLNSAYGSEHLLKSLLRKMKQYKVRAMADIVINHRVGTTR 120

Query: 121 GHGGMHNRFDGILLPWDEHAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 180
           GHGGM+NR+DGI LPWDEHAVTSC+GGLGNRSTGDNF+GVPN+DHTQ FVR+DII WL+W
Sbjct: 121 GHGGMYNRYDGISLPWDEHAVTSCTGGLGNRSTGDNFNGVPNVDHTQHFVRKDIIGWLRW 180

Query: 181 LRSSVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQDGHRQR 240
           LR++VGFQDFRFDFARG+SA +VKEYI AAKP+FS+GE WDSCNYNGH LDYNQD HRQR
Sbjct: 181 LRNTVGFQDFRFDFARGYSANYVKEYIGAAKPLFSVGECWDSCNYNGHGLDYNQDSHRQR 240

Query: 241 IINWIDGTGQLSAAFDFTTKGVLQEAVKGQLWRLCDCRGKPPGVMGWWPSRAVTFLDNHD 300
           II+WID TGQ+SAAFDFTTKG+LQEAVKGQ WRLCD +GKPPGVMGWWPSRAVTFLDNHD
Sbjct: 241 IISWIDATGQISAAFDFTTKGILQEAVKGQYWRLCDAQGKPPGVMGWWPSRAVTFLDNHD 300

Query: 301 TGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIDIRKHQDIN 360
           TGSTQAHWPFP++H+MEGYAYILTHPGIP VFYDHFYDWG S+HD+IVKLIDIR+ QDI+
Sbjct: 301 TGSTQAHWPFPSHHVMEGYAYILTHPGIPCVFYDHFYDWGSSIHDQIVKLIDIRRRQDIH 360

Query: 361 SRSSVKILEARSDLYSAIIGENLCMKIGDGSWCP-GTEWTLATYGCRYAVWQK 413
           SRS+V++L+A S+LY+AI+GE +CMK+GDGSWCP G +WTLAT G RYAVW K
Sbjct: 361 SRSTVRVLKAESNLYAAIVGEKICMKLGDGSWCPSGRDWTLATSGHRYAVWHK 413

BLAST of IVF0018661 vs. ExPASy Swiss-Prot
Match: Q94A41 (Alpha-amylase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=AMY3 PE=1 SV=1)

HSP 1 Score: 421.8 bits (1083), Expect = 9.0e-117
Identity = 198/397 (49.87%), Postives = 264/397 (66.50%), Query Frame = 0

Query: 23  NGREILFQAFNWESHKCD-WSGNLECKVHDIAKSGFTSAWLPPPTHSIAPQGYLPQNLYS 82
           +G EIL Q FNWES+K   W   L+ K  ++A  GFT  WLPPPT S++P+GY+P++LY+
Sbjct: 493 SGFEILCQGFNWESNKSGRWYLELQEKADELASLGFTVLWLPPPTESVSPEGYMPKDLYN 552

Query: 83  LNSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVVGHGGMHNRFDGILLPWDEHAV 142
           LNS YG+ D LK  + K  +  ++ + D V+NHR        G+ N F G  L WD+ AV
Sbjct: 553 LNSRYGTIDELKDTVKKFHKVGIKVLGDAVLNHRCAHFKNQNGVWNLFGG-RLNWDDRAV 612

Query: 143 TSCS---GGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKWLRSSVGFQDFRFDFARGF 202
            +      G GN+S+GDNFH  PNIDH+Q FVR+DI  WL W+   VG+  +R DF RGF
Sbjct: 613 VADDPHFQGRGNKSSGDNFHAAPNIDHSQDFVRKDIKEWLCWMMEEVGYDGWRLDFVRGF 672

Query: 203 SAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQDGHRQRIINWIDGTGQLSAAFDFT 262
              +VK+Y++A+KP F++GEYWDS +Y   ++DYNQD HRQRI++WI+ T   + AFD T
Sbjct: 673 WGGYVKDYMDASKPYFAVGEYWDSLSYTYGEMDYNQDAHRQRIVDWINATSGAAGAFDVT 732

Query: 263 TKGVLQEAV-KGQLWRLCDCRGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPANHIME 322
           TKG+L  A+ K + WRL D +GKPPGV+GWWPSRAVTF++NHDTGSTQ HW FP    M+
Sbjct: 733 TKGILHTALQKCEYWRLSDPKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPEGKEMQ 792

Query: 323 GYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIDIRKHQDINSRSSVKILEARSDLYSA 382
           GYAYILTHPG P VF+DH +      H EI  L+ +R  Q ++ RS V I ++  D+Y+A
Sbjct: 793 GYAYILTHPGTPAVFFDHIF---SDYHSEIAALLSLRNRQKLHCRSEVNIDKSERDVYAA 852

Query: 383 IIGENLCMKIGDGSWCP---GTEWTLATYGCRYAVWQ 412
           II E + MKIG G + P      W++A  G  Y VW+
Sbjct: 853 IIDEKVAMKIGPGHYEPPNGSQNWSVAVEGRDYKVWE 885

BLAST of IVF0018661 vs. ExPASy Swiss-Prot
Match: P27934 (Alpha-amylase isozyme 3E OS=Oryza sativa subsp. japonica OX=39947 GN=AMY1.4 PE=2 SV=1)

HSP 1 Score: 379.8 bits (974), Expect = 3.9e-104
Identity = 184/402 (45.77%), Postives = 254/402 (63.18%), Query Frame = 0

Query: 26  EILFQAFNWESHKCD--WSGNLECKVHDIAKSGFTSAWLPPPTHSIAPQGYLPQNLYSLN 85
           ++LFQ FNWES +    W   L  KV +IA +G T  WLPPP+HS++PQGY+P  LY L+
Sbjct: 26  QVLFQGFNWESWRKQGGWYNFLHEKVEEIASTGATHVWLPPPSHSVSPQGYMPGRLYDLD 85

Query: 86  SS-YGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVVGHGGMHNRFDGIL----LPWDE 145
           +S YG+E  LK+L+       V  +ADIVINHR        G++  F+G      L W  
Sbjct: 86  ASKYGTEAELKSLIEAFHDKNVECLADIVINHRCADYKDSRGVYCVFEGGTPDGRLDWGP 145

Query: 146 HAV----TSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKWLRSSVGFQDFRFDF 205
             +    T  S G G+R TG  F   P+IDH  P V+R++  WL WLR+ +GF  +R DF
Sbjct: 146 DMICSDDTQYSNGRGHRDTGAGFGAAPDIDHLNPRVQRELTDWLNWLRTDLGFDGWRLDF 205

Query: 206 ARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDL-DYNQDGHRQRIINWIDGTGQLSA 265
           A+G+SA   + Y++   P F +GE W S  YNG      NQD  RQ ++NW++G G+ + 
Sbjct: 206 AKGYSAPLARIYVDNTNPTFVVGEIWSSLIYNGDGKPSTNQDADRQELVNWVEGVGKPAT 265

Query: 266 AFDFTTKGVLQEAVKGQLWRLCDCRGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPAN 325
           AFDFTTKG+LQ AV+G+LWRL D  GK PG+MGW P +AVTF+DNHDTGSTQ+ WPFP++
Sbjct: 266 AFDFTTKGILQAAVQGELWRLHDGNGKAPGLMGWMPDQAVTFVDNHDTGSTQSLWPFPSD 325

Query: 326 HIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIDIRKHQDINSRSSVKILEARSD 385
            +M+GYAYILTHPGIP +FYDH +DW  ++  EI  L +IR    I++ S++ IL+A  D
Sbjct: 326 KVMQGYAYILTHPGIPCIFYDHVFDW--NLQHEIATLAEIRSRNGIHAESTLDILKAEGD 385

Query: 386 LYSAIIGENLCMKIG---DGSWCPGTEWTLATYGCRYAVWQK 413
           +Y A+I   +  K+G   D      +++ +  +G  Y VW+K
Sbjct: 386 IYVAMIDGKVITKLGPRYDAGGIIPSDFHVVAHGNDYCVWEK 425

BLAST of IVF0018661 vs. ExPASy Swiss-Prot
Match: P17859 (Alpha-amylase OS=Vigna mungo OX=3915 GN=AMY1.1 PE=2 SV=1)

HSP 1 Score: 378.6 bits (971), Expect = 8.8e-104
Identity = 184/401 (45.89%), Postives = 259/401 (64.59%), Query Frame = 0

Query: 27  ILFQAFNWE-SHKCDWSGNLECKVHDIAKSGFTSAWLPPPTHSIAPQGYLPQNLYSLNSS 86
           +LFQ FNWE S K  W  +L+  + D+A +G T  WLPPP+ S++P+GYLP  LY L++S
Sbjct: 25  LLFQGFNWESSKKGGWYNSLKNSIPDLANAGITHVWLPPPSQSVSPEGYLPGRLYDLDAS 84

Query: 87  -YGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVVGHGGMHNRFDG----ILLPWDEHA 146
            YGS++ LK+L+    +  ++ +ADIVINHR        G++  F+G        W    
Sbjct: 85  KYGSKNELKSLIAAFHEKGIKCLADIVINHRTAERKDGRGIYCIFEGGTPDSRQDWGPSF 144

Query: 147 V----TSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKWLRSSVGFQDFRFDFAR 206
           +    T+ S G GN  +G+ +   P+IDH  P V+R++  W+ WL++ +GF  +RFDF +
Sbjct: 145 ICRDDTAYSDGTGNNDSGEGYDAAPDIDHLNPQVQRELSEWMNWLKTEIGFDGWRFDFVK 204

Query: 207 GFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHD--LDYNQDGHRQRIINWIDGTGQLSAA 266
           G++    K Y+E  KP F++GE WDS +Y G D   +YNQD HR  ++NW++  G    A
Sbjct: 205 GYAPSISKIYMEQTKPDFAVGEKWDSISY-GQDGKPNYNQDSHRGALVNWVESAGGAITA 264

Query: 267 FDFTTKGVLQEAVKGQLWRLCDCRGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPANH 326
           FDFTTKG+LQ AV+G+LWRL D  GKPPG++G  P  AVTF+DNHDTGSTQ  WPFP++ 
Sbjct: 265 FDFTTKGILQAAVQGELWRLIDPNGKPPGMIGVKPENAVTFIDNHDTGSTQRLWPFPSDK 324

Query: 327 IMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIDIRKHQDINSRSSVKILEARSDL 386
           +M+GYAYILTHPG P++FYDHF+DWG  + ++I KL  IR    IN +S+VKI+ +  DL
Sbjct: 325 VMQGYAYILTHPGTPSIFYDHFFDWG--LKEQIAKLSSIRLRNGINEKSTVKIMASEGDL 384

Query: 387 YSAIIGENLCMKIGD----GSWCPGTEWTLATYGCRYAVWQ 412
           Y A I   + +KIG     G+  P +   +AT G  YAVW+
Sbjct: 385 YVAKIDNKIMVKIGPKMDLGNLIP-SNLHVATSGQDYAVWE 421

BLAST of IVF0018661 vs. ExPASy Swiss-Prot
Match: P27932 (Alpha-amylase isozyme 3A OS=Oryza sativa subsp. japonica OX=39947 GN=AMY1.2 PE=2 SV=2)

HSP 1 Score: 376.3 bits (965), Expect = 4.4e-103
Identity = 183/403 (45.41%), Postives = 258/403 (64.02%), Query Frame = 0

Query: 26  EILFQAFNWESHKCD--WSGNLECKVHDIAKSGFTSAWLPPPTHSIAPQGYLPQNLYSLN 85
           +ILFQ FNW+S K    W   L+ +V DIA +G T  WLPPPTHS++PQGY+P  LY LN
Sbjct: 29  QILFQGFNWDSWKKQGGWYNMLKDQVGDIASAGVTHVWLPPPTHSVSPQGYMPGRLYDLN 88

Query: 86  SS-YGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVVGHGGMHNRFDG----ILLPWDE 145
           +S YG++  LK+L+       ++ +ADIV+NHR        G++  F G      L W  
Sbjct: 89  ASKYGTKAELKSLIAAFHAKGIKCVADIVVNHRCADDKDGRGVYCIFKGGGPRGCLDWGP 148

Query: 146 HAV----TSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKWLRSSVGFQDFRFDF 205
             +    T  S G G+R TG +F   P+IDH  P V+R++  WL+WLR  VGF  +R DF
Sbjct: 149 SMICCDDTQYSDGTGHRDTGADFAAAPDIDHLNPLVQRELSDWLRWLRRDVGFDGWRLDF 208

Query: 206 ARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLD-YNQDGHRQRIINWIDGTGQLSA 265
           A+G+SA   + Y++ A+P F + E W+S +Y+G      NQDG RQ ++NW+   G  + 
Sbjct: 209 AKGYSAAVARTYVQNARPSFVVAEIWNSLSYDGDGKPAANQDGQRQELVNWVKQVGGPAT 268

Query: 266 AFDFTTKGVLQEAVKGQLWRLCDCRGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPAN 325
           AFDFTTKG+LQ AV+G+LWR+ D  GK PG++GW+P +AVTF+DNHDTGSTQ  WPFP++
Sbjct: 269 AFDFTTKGILQSAVQGELWRMRDKDGKAPGMIGWYPEKAVTFVDNHDTGSTQRMWPFPSD 328

Query: 326 HIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIDIRKHQDINSRSSVKILEARSD 385
            ++ GYAYILTHPG+P +FYDH +DW  ++  EI  L   RK   IN+ S +++L A SD
Sbjct: 329 KVILGYAYILTHPGVPCIFYDHVFDW--NLKQEINALAATRKRNGINAGSKLRVLAAESD 388

Query: 386 LYSAIIGENLCMKIGD----GSWCPGTEWTLATYGCRYAVWQK 413
           +Y A++ E +  KIG     G+  P +++ +  +G  Y VW+K
Sbjct: 389 MYVAMVDERVITKIGPRIDVGNIIP-SDFHIVAHGNDYCVWEK 428

BLAST of IVF0018661 vs. ExPASy TrEMBL
Match: A0A5A7VG02 (Alpha-amylase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold255G003440 PE=3 SV=1)

HSP 1 Score: 899.0 bits (2322), Expect = 7.1e-258
Identity = 412/412 (100.00%), Postives = 412/412 (100.00%), Query Frame = 0

Query: 1   MGDHDNVFDENYEQTDTGAVIRNGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSA 60
           MGDHDNVFDENYEQTDTGAVIRNGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSA
Sbjct: 56  MGDHDNVFDENYEQTDTGAVIRNGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSA 115

Query: 61  WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVV 120
           WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVV
Sbjct: 116 WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVV 175

Query: 121 GHGGMHNRFDGILLPWDEHAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 180
           GHGGMHNRFDGILLPWDEHAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW
Sbjct: 176 GHGGMHNRFDGILLPWDEHAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 235

Query: 181 LRSSVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQDGHRQR 240
           LRSSVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQDGHRQR
Sbjct: 236 LRSSVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQDGHRQR 295

Query: 241 IINWIDGTGQLSAAFDFTTKGVLQEAVKGQLWRLCDCRGKPPGVMGWWPSRAVTFLDNHD 300
           IINWIDGTGQLSAAFDFTTKGVLQEAVKGQLWRLCDCRGKPPGVMGWWPSRAVTFLDNHD
Sbjct: 296 IINWIDGTGQLSAAFDFTTKGVLQEAVKGQLWRLCDCRGKPPGVMGWWPSRAVTFLDNHD 355

Query: 301 TGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIDIRKHQDIN 360
           TGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIDIRKHQDIN
Sbjct: 356 TGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIDIRKHQDIN 415

Query: 361 SRSSVKILEARSDLYSAIIGENLCMKIGDGSWCPGTEWTLATYGCRYAVWQK 413
           SRSSVKILEARSDLYSAIIGENLCMKIGDGSWCPGTEWTLATYGCRYAVWQK
Sbjct: 416 SRSSVKILEARSDLYSAIIGENLCMKIGDGSWCPGTEWTLATYGCRYAVWQK 467

BLAST of IVF0018661 vs. ExPASy TrEMBL
Match: A0A1S3BW59 (Alpha-amylase OS=Cucumis melo OX=3656 GN=LOC103493790 PE=3 SV=1)

HSP 1 Score: 899.0 bits (2322), Expect = 7.1e-258
Identity = 412/412 (100.00%), Postives = 412/412 (100.00%), Query Frame = 0

Query: 1   MGDHDNVFDENYEQTDTGAVIRNGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSA 60
           MGDHDNVFDENYEQTDTGAVIRNGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSA
Sbjct: 26  MGDHDNVFDENYEQTDTGAVIRNGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSA 85

Query: 61  WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVV 120
           WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVV
Sbjct: 86  WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVV 145

Query: 121 GHGGMHNRFDGILLPWDEHAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 180
           GHGGMHNRFDGILLPWDEHAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW
Sbjct: 146 GHGGMHNRFDGILLPWDEHAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 205

Query: 181 LRSSVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQDGHRQR 240
           LRSSVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQDGHRQR
Sbjct: 206 LRSSVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQDGHRQR 265

Query: 241 IINWIDGTGQLSAAFDFTTKGVLQEAVKGQLWRLCDCRGKPPGVMGWWPSRAVTFLDNHD 300
           IINWIDGTGQLSAAFDFTTKGVLQEAVKGQLWRLCDCRGKPPGVMGWWPSRAVTFLDNHD
Sbjct: 266 IINWIDGTGQLSAAFDFTTKGVLQEAVKGQLWRLCDCRGKPPGVMGWWPSRAVTFLDNHD 325

Query: 301 TGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIDIRKHQDIN 360
           TGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIDIRKHQDIN
Sbjct: 326 TGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIDIRKHQDIN 385

Query: 361 SRSSVKILEARSDLYSAIIGENLCMKIGDGSWCPGTEWTLATYGCRYAVWQK 413
           SRSSVKILEARSDLYSAIIGENLCMKIGDGSWCPGTEWTLATYGCRYAVWQK
Sbjct: 386 SRSSVKILEARSDLYSAIIGENLCMKIGDGSWCPGTEWTLATYGCRYAVWQK 437

BLAST of IVF0018661 vs. ExPASy TrEMBL
Match: A0A5D3D8R2 (Alpha-amylase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G00850 PE=3 SV=1)

HSP 1 Score: 893.3 bits (2307), Expect = 3.9e-256
Identity = 412/416 (99.04%), Postives = 412/416 (99.04%), Query Frame = 0

Query: 1   MGDHDNVFDENYEQTDTGAVIRNGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSA 60
           MGDHDNVFDENYEQTDTGAVIRNGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSA
Sbjct: 56  MGDHDNVFDENYEQTDTGAVIRNGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSA 115

Query: 61  WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVV 120
           WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVV
Sbjct: 116 WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVV 175

Query: 121 GHGGMHNRFDGILLPWDEHAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 180
           GHGGMHNRFDGILLPWDEHAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW
Sbjct: 176 GHGGMHNRFDGILLPWDEHAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 235

Query: 181 LRSSVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQ----DG 240
           LRSSVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQ    DG
Sbjct: 236 LRSSVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQALNADG 295

Query: 241 HRQRIINWIDGTGQLSAAFDFTTKGVLQEAVKGQLWRLCDCRGKPPGVMGWWPSRAVTFL 300
           HRQRIINWIDGTGQLSAAFDFTTKGVLQEAVKGQLWRLCDCRGKPPGVMGWWPSRAVTFL
Sbjct: 296 HRQRIINWIDGTGQLSAAFDFTTKGVLQEAVKGQLWRLCDCRGKPPGVMGWWPSRAVTFL 355

Query: 301 DNHDTGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIDIRKH 360
           DNHDTGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIDIRKH
Sbjct: 356 DNHDTGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIDIRKH 415

Query: 361 QDINSRSSVKILEARSDLYSAIIGENLCMKIGDGSWCPGTEWTLATYGCRYAVWQK 413
           QDINSRSSVKILEARSDLYSAIIGENLCMKIGDGSWCPGTEWTLATYGCRYAVWQK
Sbjct: 416 QDINSRSSVKILEARSDLYSAIIGENLCMKIGDGSWCPGTEWTLATYGCRYAVWQK 471

BLAST of IVF0018661 vs. ExPASy TrEMBL
Match: E5GCD3 (Alpha-amylase OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1)

HSP 1 Score: 893.3 bits (2307), Expect = 3.9e-256
Identity = 412/416 (99.04%), Postives = 412/416 (99.04%), Query Frame = 0

Query: 1   MGDHDNVFDENYEQTDTGAVIRNGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSA 60
           MGDHDNVFDENYEQTDTGAVIRNGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSA
Sbjct: 1   MGDHDNVFDENYEQTDTGAVIRNGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSA 60

Query: 61  WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVV 120
           WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVV
Sbjct: 61  WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVV 120

Query: 121 GHGGMHNRFDGILLPWDEHAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 180
           GHGGMHNRFDGILLPWDEHAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW
Sbjct: 121 GHGGMHNRFDGILLPWDEHAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 180

Query: 181 LRSSVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQ----DG 240
           LRSSVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQ    DG
Sbjct: 181 LRSSVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQALNADG 240

Query: 241 HRQRIINWIDGTGQLSAAFDFTTKGVLQEAVKGQLWRLCDCRGKPPGVMGWWPSRAVTFL 300
           HRQRIINWIDGTGQLSAAFDFTTKGVLQEAVKGQLWRLCDCRGKPPGVMGWWPSRAVTFL
Sbjct: 241 HRQRIINWIDGTGQLSAAFDFTTKGVLQEAVKGQLWRLCDCRGKPPGVMGWWPSRAVTFL 300

Query: 301 DNHDTGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIDIRKH 360
           DNHDTGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIDIRKH
Sbjct: 301 DNHDTGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIDIRKH 360

Query: 361 QDINSRSSVKILEARSDLYSAIIGENLCMKIGDGSWCPGTEWTLATYGCRYAVWQK 413
           QDINSRSSVKILEARSDLYSAIIGENLCMKIGDGSWCPGTEWTLATYGCRYAVWQK
Sbjct: 361 QDINSRSSVKILEARSDLYSAIIGENLCMKIGDGSWCPGTEWTLATYGCRYAVWQK 416

BLAST of IVF0018661 vs. ExPASy TrEMBL
Match: A0A1S4DZE2 (Alpha-amylase OS=Cucumis melo OX=3656 GN=LOC103493790 PE=3 SV=1)

HSP 1 Score: 891.7 bits (2303), Expect = 1.1e-255
Identity = 412/420 (98.10%), Postives = 412/420 (98.10%), Query Frame = 0

Query: 1   MGDHDNVFDENYEQTDTGAVIRNGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSA 60
           MGDHDNVFDENYEQTDTGAVIRNGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSA
Sbjct: 1   MGDHDNVFDENYEQTDTGAVIRNGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSA 60

Query: 61  WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVV 120
           WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVV
Sbjct: 61  WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVV 120

Query: 121 GHGGMHNRFDGILLPWDEHAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 180
           GHGGMHNRFDGILLPWDEHAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW
Sbjct: 121 GHGGMHNRFDGILLPWDEHAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 180

Query: 181 LRSSVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQ------ 240
           LRSSVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQ      
Sbjct: 181 LRSSVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQVMITAL 240

Query: 241 --DGHRQRIINWIDGTGQLSAAFDFTTKGVLQEAVKGQLWRLCDCRGKPPGVMGWWPSRA 300
             DGHRQRIINWIDGTGQLSAAFDFTTKGVLQEAVKGQLWRLCDCRGKPPGVMGWWPSRA
Sbjct: 241 NADGHRQRIINWIDGTGQLSAAFDFTTKGVLQEAVKGQLWRLCDCRGKPPGVMGWWPSRA 300

Query: 301 VTFLDNHDTGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLID 360
           VTFLDNHDTGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLID
Sbjct: 301 VTFLDNHDTGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLID 360

Query: 361 IRKHQDINSRSSVKILEARSDLYSAIIGENLCMKIGDGSWCPGTEWTLATYGCRYAVWQK 413
           IRKHQDINSRSSVKILEARSDLYSAIIGENLCMKIGDGSWCPGTEWTLATYGCRYAVWQK
Sbjct: 361 IRKHQDINSRSSVKILEARSDLYSAIIGENLCMKIGDGSWCPGTEWTLATYGCRYAVWQK 420

BLAST of IVF0018661 vs. NCBI nr
Match: KAA0064621.1 (putative alpha-amylase 2 isoform X2 [Cucumis melo var. makuwa])

HSP 1 Score: 895 bits (2314), Expect = 0.0
Identity = 412/412 (100.00%), Postives = 412/412 (100.00%), Query Frame = 0

Query: 1   MGDHDNVFDENYEQTDTGAVIRNGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSA 60
           MGDHDNVFDENYEQTDTGAVIRNGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSA
Sbjct: 56  MGDHDNVFDENYEQTDTGAVIRNGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSA 115

Query: 61  WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVV 120
           WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVV
Sbjct: 116 WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVV 175

Query: 121 GHGGMHNRFDGILLPWDEHAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 180
           GHGGMHNRFDGILLPWDEHAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW
Sbjct: 176 GHGGMHNRFDGILLPWDEHAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 235

Query: 181 LRSSVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQDGHRQR 240
           LRSSVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQDGHRQR
Sbjct: 236 LRSSVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQDGHRQR 295

Query: 241 IINWIDGTGQLSAAFDFTTKGVLQEAVKGQLWRLCDCRGKPPGVMGWWPSRAVTFLDNHD 300
           IINWIDGTGQLSAAFDFTTKGVLQEAVKGQLWRLCDCRGKPPGVMGWWPSRAVTFLDNHD
Sbjct: 296 IINWIDGTGQLSAAFDFTTKGVLQEAVKGQLWRLCDCRGKPPGVMGWWPSRAVTFLDNHD 355

Query: 301 TGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIDIRKHQDIN 360
           TGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIDIRKHQDIN
Sbjct: 356 TGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIDIRKHQDIN 415

Query: 361 SRSSVKILEARSDLYSAIIGENLCMKIGDGSWCPGTEWTLATYGCRYAVWQK 412
           SRSSVKILEARSDLYSAIIGENLCMKIGDGSWCPGTEWTLATYGCRYAVWQK
Sbjct: 416 SRSSVKILEARSDLYSAIIGENLCMKIGDGSWCPGTEWTLATYGCRYAVWQK 467

BLAST of IVF0018661 vs. NCBI nr
Match: XP_008452915.1 (PREDICTED: probable alpha-amylase 2 isoform X2 [Cucumis melo])

HSP 1 Score: 895 bits (2314), Expect = 0.0
Identity = 412/412 (100.00%), Postives = 412/412 (100.00%), Query Frame = 0

Query: 1   MGDHDNVFDENYEQTDTGAVIRNGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSA 60
           MGDHDNVFDENYEQTDTGAVIRNGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSA
Sbjct: 26  MGDHDNVFDENYEQTDTGAVIRNGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSA 85

Query: 61  WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVV 120
           WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVV
Sbjct: 86  WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVV 145

Query: 121 GHGGMHNRFDGILLPWDEHAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 180
           GHGGMHNRFDGILLPWDEHAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW
Sbjct: 146 GHGGMHNRFDGILLPWDEHAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 205

Query: 181 LRSSVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQDGHRQR 240
           LRSSVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQDGHRQR
Sbjct: 206 LRSSVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQDGHRQR 265

Query: 241 IINWIDGTGQLSAAFDFTTKGVLQEAVKGQLWRLCDCRGKPPGVMGWWPSRAVTFLDNHD 300
           IINWIDGTGQLSAAFDFTTKGVLQEAVKGQLWRLCDCRGKPPGVMGWWPSRAVTFLDNHD
Sbjct: 266 IINWIDGTGQLSAAFDFTTKGVLQEAVKGQLWRLCDCRGKPPGVMGWWPSRAVTFLDNHD 325

Query: 301 TGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIDIRKHQDIN 360
           TGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIDIRKHQDIN
Sbjct: 326 TGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIDIRKHQDIN 385

Query: 361 SRSSVKILEARSDLYSAIIGENLCMKIGDGSWCPGTEWTLATYGCRYAVWQK 412
           SRSSVKILEARSDLYSAIIGENLCMKIGDGSWCPGTEWTLATYGCRYAVWQK
Sbjct: 386 SRSSVKILEARSDLYSAIIGENLCMKIGDGSWCPGTEWTLATYGCRYAVWQK 437

BLAST of IVF0018661 vs. NCBI nr
Match: ADN34132.1 (cytosolic alpha-amylase [Cucumis melo subsp. melo])

HSP 1 Score: 890 bits (2299), Expect = 0.0
Identity = 412/416 (99.04%), Postives = 412/416 (99.04%), Query Frame = 0

Query: 1   MGDHDNVFDENYEQTDTGAVIRNGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSA 60
           MGDHDNVFDENYEQTDTGAVIRNGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSA
Sbjct: 1   MGDHDNVFDENYEQTDTGAVIRNGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSA 60

Query: 61  WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVV 120
           WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVV
Sbjct: 61  WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVV 120

Query: 121 GHGGMHNRFDGILLPWDEHAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 180
           GHGGMHNRFDGILLPWDEHAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW
Sbjct: 121 GHGGMHNRFDGILLPWDEHAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 180

Query: 181 LRSSVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQ----DG 240
           LRSSVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQ    DG
Sbjct: 181 LRSSVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQALNADG 240

Query: 241 HRQRIINWIDGTGQLSAAFDFTTKGVLQEAVKGQLWRLCDCRGKPPGVMGWWPSRAVTFL 300
           HRQRIINWIDGTGQLSAAFDFTTKGVLQEAVKGQLWRLCDCRGKPPGVMGWWPSRAVTFL
Sbjct: 241 HRQRIINWIDGTGQLSAAFDFTTKGVLQEAVKGQLWRLCDCRGKPPGVMGWWPSRAVTFL 300

Query: 301 DNHDTGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIDIRKH 360
           DNHDTGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIDIRKH
Sbjct: 301 DNHDTGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIDIRKH 360

Query: 361 QDINSRSSVKILEARSDLYSAIIGENLCMKIGDGSWCPGTEWTLATYGCRYAVWQK 412
           QDINSRSSVKILEARSDLYSAIIGENLCMKIGDGSWCPGTEWTLATYGCRYAVWQK
Sbjct: 361 QDINSRSSVKILEARSDLYSAIIGENLCMKIGDGSWCPGTEWTLATYGCRYAVWQK 416

BLAST of IVF0018661 vs. NCBI nr
Match: TYK19971.1 (putative alpha-amylase 2 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 890 bits (2299), Expect = 0.0
Identity = 412/416 (99.04%), Postives = 412/416 (99.04%), Query Frame = 0

Query: 1   MGDHDNVFDENYEQTDTGAVIRNGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSA 60
           MGDHDNVFDENYEQTDTGAVIRNGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSA
Sbjct: 56  MGDHDNVFDENYEQTDTGAVIRNGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSA 115

Query: 61  WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVV 120
           WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVV
Sbjct: 116 WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVV 175

Query: 121 GHGGMHNRFDGILLPWDEHAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 180
           GHGGMHNRFDGILLPWDEHAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW
Sbjct: 176 GHGGMHNRFDGILLPWDEHAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 235

Query: 181 LRSSVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQ----DG 240
           LRSSVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQ    DG
Sbjct: 236 LRSSVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQALNADG 295

Query: 241 HRQRIINWIDGTGQLSAAFDFTTKGVLQEAVKGQLWRLCDCRGKPPGVMGWWPSRAVTFL 300
           HRQRIINWIDGTGQLSAAFDFTTKGVLQEAVKGQLWRLCDCRGKPPGVMGWWPSRAVTFL
Sbjct: 296 HRQRIINWIDGTGQLSAAFDFTTKGVLQEAVKGQLWRLCDCRGKPPGVMGWWPSRAVTFL 355

Query: 301 DNHDTGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIDIRKH 360
           DNHDTGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIDIRKH
Sbjct: 356 DNHDTGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIDIRKH 415

Query: 361 QDINSRSSVKILEARSDLYSAIIGENLCMKIGDGSWCPGTEWTLATYGCRYAVWQK 412
           QDINSRSSVKILEARSDLYSAIIGENLCMKIGDGSWCPGTEWTLATYGCRYAVWQK
Sbjct: 416 QDINSRSSVKILEARSDLYSAIIGENLCMKIGDGSWCPGTEWTLATYGCRYAVWQK 471

BLAST of IVF0018661 vs. NCBI nr
Match: XP_016901343.1 (PREDICTED: probable alpha-amylase 2 isoform X1 [Cucumis melo])

HSP 1 Score: 888 bits (2295), Expect = 0.0
Identity = 412/420 (98.10%), Postives = 412/420 (98.10%), Query Frame = 0

Query: 1   MGDHDNVFDENYEQTDTGAVIRNGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSA 60
           MGDHDNVFDENYEQTDTGAVIRNGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSA
Sbjct: 26  MGDHDNVFDENYEQTDTGAVIRNGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSA 85

Query: 61  WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVV 120
           WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVV
Sbjct: 86  WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVV 145

Query: 121 GHGGMHNRFDGILLPWDEHAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 180
           GHGGMHNRFDGILLPWDEHAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW
Sbjct: 146 GHGGMHNRFDGILLPWDEHAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 205

Query: 181 LRSSVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQ------ 240
           LRSSVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQ      
Sbjct: 206 LRSSVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQVMITAL 265

Query: 241 --DGHRQRIINWIDGTGQLSAAFDFTTKGVLQEAVKGQLWRLCDCRGKPPGVMGWWPSRA 300
             DGHRQRIINWIDGTGQLSAAFDFTTKGVLQEAVKGQLWRLCDCRGKPPGVMGWWPSRA
Sbjct: 266 NADGHRQRIINWIDGTGQLSAAFDFTTKGVLQEAVKGQLWRLCDCRGKPPGVMGWWPSRA 325

Query: 301 VTFLDNHDTGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLID 360
           VTFLDNHDTGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLID
Sbjct: 326 VTFLDNHDTGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLID 385

Query: 361 IRKHQDINSRSSVKILEARSDLYSAIIGENLCMKIGDGSWCPGTEWTLATYGCRYAVWQK 412
           IRKHQDINSRSSVKILEARSDLYSAIIGENLCMKIGDGSWCPGTEWTLATYGCRYAVWQK
Sbjct: 386 IRKHQDINSRSSVKILEARSDLYSAIIGENLCMKIGDGSWCPGTEWTLATYGCRYAVWQK 445

BLAST of IVF0018661 vs. TAIR 10
Match: AT1G76130.1 (alpha-amylase-like 2 )

HSP 1 Score: 731.1 bits (1886), Expect = 4.9e-211
Identity = 326/413 (78.93%), Postives = 375/413 (90.80%), Query Frame = 0

Query: 1   MGDHDNVFDENYEQTDTGAVIRNGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSA 60
           MG ++NVFDE  +QTD G VIR+GRE++ QA+NWESHK DW  NL+ KV DIAKSGFTSA
Sbjct: 1   MGYYNNVFDECNDQTDIGRVIRDGREVILQAYNWESHKYDWWRNLDGKVPDIAKSGFTSA 60

Query: 61  WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVV 120
           WLPPP+ S+AP+GYLPQ+LYSLNS+YGSE +LK+LL KMKQ KVRAMADIVINHRVGT  
Sbjct: 61  WLPPPSQSLAPEGYLPQDLYSLNSAYGSEHLLKSLLRKMKQYKVRAMADIVINHRVGTTR 120

Query: 121 GHGGMHNRFDGILLPWDEHAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 180
           GHGGM+NR+DGI LPWDEHAVTSC+GGLGNRSTGDNF+GVPN+DHTQ FVR+DII WL+W
Sbjct: 121 GHGGMYNRYDGISLPWDEHAVTSCTGGLGNRSTGDNFNGVPNVDHTQHFVRKDIIGWLRW 180

Query: 181 LRSSVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQDGHRQR 240
           LR++VGFQDFRFDFARG+SA +VKEYI AAKP+FS+GE WDSCNYNGH LDYNQD HRQR
Sbjct: 181 LRNTVGFQDFRFDFARGYSANYVKEYIGAAKPLFSVGECWDSCNYNGHGLDYNQDSHRQR 240

Query: 241 IINWIDGTGQLSAAFDFTTKGVLQEAVKGQLWRLCDCRGKPPGVMGWWPSRAVTFLDNHD 300
           II+WID TGQ+SAAFDFTTKG+LQEAVKGQ WRLCD +GKPPGVMGWWPSRAVTFLDNHD
Sbjct: 241 IISWIDATGQISAAFDFTTKGILQEAVKGQYWRLCDAQGKPPGVMGWWPSRAVTFLDNHD 300

Query: 301 TGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIDIRKHQDIN 360
           TGSTQAHWPFP++H+MEGYAYILTHPGIP VFYDHFYDWG S+HD+IVKLIDIR+ QDI+
Sbjct: 301 TGSTQAHWPFPSHHVMEGYAYILTHPGIPCVFYDHFYDWGSSIHDQIVKLIDIRRRQDIH 360

Query: 361 SRSSVKILEARSDLYSAIIGENLCMKIGDGSWCP-GTEWTLATYGCRYAVWQK 413
           SRS+V++L+A S+LY+AI+GE +CMK+GDGSWCP G +WTLAT G RYAVW K
Sbjct: 361 SRSTVRVLKAESNLYAAIVGEKICMKLGDGSWCPSGRDWTLATSGHRYAVWHK 413

BLAST of IVF0018661 vs. TAIR 10
Match: AT1G69830.1 (alpha-amylase-like 3 )

HSP 1 Score: 421.8 bits (1083), Expect = 6.4e-118
Identity = 198/397 (49.87%), Postives = 264/397 (66.50%), Query Frame = 0

Query: 23  NGREILFQAFNWESHKCD-WSGNLECKVHDIAKSGFTSAWLPPPTHSIAPQGYLPQNLYS 82
           +G EIL Q FNWES+K   W   L+ K  ++A  GFT  WLPPPT S++P+GY+P++LY+
Sbjct: 493 SGFEILCQGFNWESNKSGRWYLELQEKADELASLGFTVLWLPPPTESVSPEGYMPKDLYN 552

Query: 83  LNSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVVGHGGMHNRFDGILLPWDEHAV 142
           LNS YG+ D LK  + K  +  ++ + D V+NHR        G+ N F G  L WD+ AV
Sbjct: 553 LNSRYGTIDELKDTVKKFHKVGIKVLGDAVLNHRCAHFKNQNGVWNLFGG-RLNWDDRAV 612

Query: 143 TSCS---GGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKWLRSSVGFQDFRFDFARGF 202
            +      G GN+S+GDNFH  PNIDH+Q FVR+DI  WL W+   VG+  +R DF RGF
Sbjct: 613 VADDPHFQGRGNKSSGDNFHAAPNIDHSQDFVRKDIKEWLCWMMEEVGYDGWRLDFVRGF 672

Query: 203 SAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQDGHRQRIINWIDGTGQLSAAFDFT 262
              +VK+Y++A+KP F++GEYWDS +Y   ++DYNQD HRQRI++WI+ T   + AFD T
Sbjct: 673 WGGYVKDYMDASKPYFAVGEYWDSLSYTYGEMDYNQDAHRQRIVDWINATSGAAGAFDVT 732

Query: 263 TKGVLQEAV-KGQLWRLCDCRGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPANHIME 322
           TKG+L  A+ K + WRL D +GKPPGV+GWWPSRAVTF++NHDTGSTQ HW FP    M+
Sbjct: 733 TKGILHTALQKCEYWRLSDPKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPEGKEMQ 792

Query: 323 GYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIDIRKHQDINSRSSVKILEARSDLYSA 382
           GYAYILTHPG P VF+DH +      H EI  L+ +R  Q ++ RS V I ++  D+Y+A
Sbjct: 793 GYAYILTHPGTPAVFFDHIF---SDYHSEIAALLSLRNRQKLHCRSEVNIDKSERDVYAA 852

Query: 383 IIGENLCMKIGDGSWCP---GTEWTLATYGCRYAVWQ 412
           II E + MKIG G + P      W++A  G  Y VW+
Sbjct: 853 IIDEKVAMKIGPGHYEPPNGSQNWSVAVEGRDYKVWE 885

BLAST of IVF0018661 vs. TAIR 10
Match: AT4G25000.1 (alpha-amylase-like )

HSP 1 Score: 351.3 bits (900), Expect = 1.1e-96
Identity = 182/402 (45.27%), Postives = 250/402 (62.19%), Query Frame = 0

Query: 27  ILFQAFNWESHKCD--WSGNLECKVHDIAKSGFTSAWLPPPTHSIAPQGYLPQNLYSLNS 86
           +LFQ+FNWES K +  +  +L   + DIA +G T  WLPPP+ S+AP+GYLP  LY LNS
Sbjct: 27  LLFQSFNWESWKKEGGFYNSLHNSIDDIANAGITHLWLPPPSQSVAPEGYLPGKLYDLNS 86

Query: 87  S-YGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVVGHGGMHNRFDG----ILLPWDEH 146
           S YGSE  LK+L+  + Q  ++A+ADIVINHR          +  F+G      L WD  
Sbjct: 87  SKYGSEAELKSLIKALNQKGIKALADIVINHRTAERKDDKCGYCYFEGGTSDDRLDWDPS 146

Query: 147 AVTSCS---GGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKWLRSSVGFQDFRFDFAR 206
            V        G GN  TG +F G P+IDH  P V++++  W+ WL++ +GF  +RFD+ R
Sbjct: 147 FVCRNDPKFPGTGNLDTGGDFDGAPDIDHLNPRVQKELSEWMNWLKTEIGFHGWRFDYVR 206

Query: 207 GFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGH-DLDYNQDGHRQRIINWI-DGTGQLSAA 266
           G+++   K Y++   P F++GE WD   Y G   LDY+Q+ HR  +  WI +  G +  A
Sbjct: 207 GYASSITKLYVQNTSPDFAVGEKWDDMKYGGDGKLDYDQNEHRSGLKQWIEEAGGGVLTA 266

Query: 267 FDFTTKGVLQEAVKGQLWRLCDCRGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPANH 326
           FDFTTKG+LQ AVKG+LWRL D +GKPPG++G  P  AVTF+DNHDT  T   W FP++ 
Sbjct: 267 FDFTTKGILQSAVKGELWRLKDSQGKPPGMIGIMPGNAVTFIDNHDTFRT---WVFPSDK 326

Query: 327 IMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIDIRKHQDINSRSSVKILEARSDL 386
           ++ GY YILTHPG P +FY+H+ +WG  + + I KL+ IR    I S SSV I  A +DL
Sbjct: 327 VLLGYVYILTHPGTPCIFYNHYIEWG--LKESISKLVAIRNKNGIGSTSSVTIKAAEADL 386

Query: 387 YSAIIGENLCMKIGD----GSWCPGTEWTLATYGCRYAVWQK 413
           Y A+I + + MKIG     G+  P + + LA  G  +AVW+K
Sbjct: 387 YLAMIDDKVIMKIGPKQDVGTLVP-SNFALAYSGLDFAVWEK 422

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8LFG17.0e-21078.93Probable alpha-amylase 2 OS=Arabidopsis thaliana OX=3702 GN=AMY2 PE=2 SV=1[more]
Q94A419.0e-11749.87Alpha-amylase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=AMY3 PE=1 SV=1[more]
P279343.9e-10445.77Alpha-amylase isozyme 3E OS=Oryza sativa subsp. japonica OX=39947 GN=AMY1.4 PE=2... [more]
P178598.8e-10445.89Alpha-amylase OS=Vigna mungo OX=3915 GN=AMY1.1 PE=2 SV=1[more]
P279324.4e-10345.41Alpha-amylase isozyme 3A OS=Oryza sativa subsp. japonica OX=39947 GN=AMY1.2 PE=2... [more]
Match NameE-valueIdentityDescription
A0A5A7VG027.1e-258100.00Alpha-amylase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold255G003440... [more]
A0A1S3BW597.1e-258100.00Alpha-amylase OS=Cucumis melo OX=3656 GN=LOC103493790 PE=3 SV=1[more]
A0A5D3D8R23.9e-25699.04Alpha-amylase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G00850 ... [more]
E5GCD33.9e-25699.04Alpha-amylase OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1[more]
A0A1S4DZE21.1e-25598.10Alpha-amylase OS=Cucumis melo OX=3656 GN=LOC103493790 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
KAA0064621.10.0100.00putative alpha-amylase 2 isoform X2 [Cucumis melo var. makuwa][more]
XP_008452915.10.0100.00PREDICTED: probable alpha-amylase 2 isoform X2 [Cucumis melo][more]
ADN34132.10.099.04cytosolic alpha-amylase [Cucumis melo subsp. melo][more]
TYK19971.10.099.04putative alpha-amylase 2 isoform X1 [Cucumis melo var. makuwa][more]
XP_016901343.10.098.10PREDICTED: probable alpha-amylase 2 isoform X1 [Cucumis melo][more]
Match NameE-valueIdentityDescription
AT1G76130.14.9e-21178.93alpha-amylase-like 2 [more]
AT1G69830.16.4e-11849.87alpha-amylase-like 3 [more]
AT4G25000.11.1e-9645.27alpha-amylase-like [more]
InterPro
Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006046Alpha amylasePRINTSPR00110ALPHAAMYLASEcoord: 103..114
score: 50.74
coord: 187..198
score: 35.54
coord: 290..302
score: 53.54
IPR012850Alpha-amylase, C-terminal beta-sheetSMARTSM00810alpha-amyl_c2coord: 355..412
e-value: 6.8E-25
score: 98.7
IPR012850Alpha-amylase, C-terminal beta-sheetPFAMPF07821Alpha-amyl_C2coord: 357..411
e-value: 1.2E-19
score: 70.3
IPR006047Glycosyl hydrolase, family 13, catalytic domainSMARTSM00642aamycoord: 26..366
e-value: 1.4E-18
score: 77.7
IPR006047Glycosyl hydrolase, family 13, catalytic domainPFAMPF00128Alpha-amylasecoord: 50..114
e-value: 2.5E-8
score: 33.8
IPR013780Glycosyl hydrolase, all-betaGENE3D2.60.40.1180coord: 359..412
e-value: 1.3E-17
score: 65.6
IPR013775Alpha-amylase, plantPIRSFPIRSF001028Alpha-amylase_plantcoord: 6..412
e-value: 9.1E-179
score: 592.7
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 26..358
e-value: 1.3E-116
score: 391.8
NoneNo IPR availablePIRSRPIRSR001021-2PIRSR001021-2coord: 161..358
e-value: 3.8E-17
score: 60.1
NoneNo IPR availablePANTHERPTHR43447ALPHA-AMYLASEcoord: 22..412
NoneNo IPR availablePANTHERPTHR43447:SF18ALPHA-AMYLASE 2-RELATEDcoord: 22..412
NoneNo IPR availableCDDcd11314AmyAc_arch_bac_plant_AmyAcoord: 27..365
e-value: 1.29838E-161
score: 454.757
NoneNo IPR availableSUPERFAMILY51011Glycosyl hydrolase domaincoord: 359..412
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 40..345

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
IVF0018661.2IVF0018661.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
molecular_function GO:0004556 alpha-amylase activity
molecular_function GO:0103025 alpha-amylase activity (releasing maltohexaose)
molecular_function GO:0005509 calcium ion binding
molecular_function GO:0003824 catalytic activity
molecular_function GO:0043169 cation binding