IVF0018306 (gene) Melon (IVF77) v1

Overview
NameIVF0018306
Typegene
OrganismCucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
DescriptionWerner Syndrome-like exonuclease
Locationtig00000135: 137903 .. 140634 (+)
RNA-Seq ExpressionIVF0018306
SyntenyIVF0018306
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAATTTCCGGGCAGTAGCAACAGTGGCCATTCTTCGACGGAGCTCTCTGCGTGCTCCTCATCTTTTCCGTCGTGGTCGTTCTCCTCATCGGAGTTTGATCAGCCGTTTACAGAGCAACAACTCCAGGCAATCGAGGCCATTGAAGCTGCTTATCAATCCACCAGTGCCAAAAAACGACGACCAAATTCCACACTCGACGAAAACGGGGTCTCATGCGTTTCTCCCAACACCGGCCGTCGACTGCCTCGTTCCATTTTTTCTCAGCAGTCTCCACATTTTTCTCCCCTGTCATCCTGTCGAGGTATTTTGTTATAAAATGTATTGTAGCAGATGCGGGGAATTTGGGGAACCATGGCTTGTGGTTTTCGAACTATCTTTTGGGGATCTAGGGTTTTTAATTCTGTGCTGAAATTGGAACTACGGGTATGTCTGTTCTGTTTTGGGTTTTAGTAAACTCGAAGATGAGGTTTCCTGCATTGAGTTATGGTGGCCGAATTATTTACAGTAGAACTGTAAGTGAGGTCGATAGAGCGTCCAGGGAGCTAGCGAAGAAAATCAACTCCACGAGGAAAGGAATGGAGCAAATTACTATTGGGTTCGACATCGAGTGGAGACCGTCATTTAAAAGAGGTCAGCAGTTGATTTGATTGAACAAACTTGCACGGGAATTTTGAAGTTTCATCCACTACTAGAAAATGCAAATGCTTCTTTCTGTTTCTTGTTACACATTCCTCTCATCTGTTGCTATTTCGCCAAACAATTGATTCGATTACGGTTTTCTTTATTCAAAAGTATTTTAGTTCTGTTTCTCGTAGGAACCAAATCTGTAGTGTCTTCAAGGAACCAGAAATATTATTTGGATTTCCTTCTCAATGTGATGATTCTTGGTTCATTGAACTTTTCTTTTTGTTAAAAGTTTCCCCTCAAGAAATTTATGCTTGACAACAGAACAAATGCATGAAAATGTTCATTTCGCCATCAATTAACTTCGATCTTGCACAACCTTATTTGATAAGATTGAAATTGTTGCTGGCTCTTATGATCTGCTAGAAGTTATTCTTGGAGAGAGCCATGAGGATAGCTCAAGTGGTCGAAACATATATATTTAACCAAAAAGTTAAAGGTTTGAATCTCCTAGCTCCCACATATTGCCGATCTAAAAGCAAAAGAAAAAGAGAAAGAAAGTGAGTCATTGTTTCTTTCTTGCGGTATTTGCTGATTGTTTAATGAAATTACGTCTGAGAATTAGACCGTGATTCTTGATTTCCCCAGTTCTTTGTGACTATGTTGCTTTATCTGGTTTTTGTGGTTAAACTCAAATCTTTGGTTGGCTGGTGGCTGGTAACTCAACAGAACTTAAGCTATTTACTCCGTTCATTCTTACAACCTGTTTAATTGGTCATGGCTTCCGCAAAACCTGTTGATCAAAACTAGAATTTCTACTTCTTTGACTCTTCTGAGATGAATTTATATGCAGGTGTTCCACCCGGAAAAGCAGCAGTTATGCAACTGTGCCTGGAAAATTCTGAATGCCATGTGATGCATATCATTCATTCGGGAATACCTCAAAGTTTGCAGGCTCTTCTAGAGGATGACACACTCAACAAGGTTTGAAACATCATTATAGTTGCTTCTATAAGTTTATTTTCATATATTACTTAGAAGTTTTAAGCCATTTTAAATCAACCATGTTGAATAAACTTCAGGCTGGAGTTGGGATTGCCAGTGATGCTTCAAAAGTTTTTTAAAGATTATAACGTGTCTATGAAACAATTGGACGAAATTTCGGATCTAGCTAATCAAAAGCTTGCTGGAGTTCCCAAAAAATGGGGTCTTCGGGCTCTAACTGAGACGCTTATATCTAAAGAGGTACTTTACTACTACTTTTGTTTGTATGAGTTAAGCTGTTTGAATTGAAATATTTCTTAAATGTAATGAAGTAAAAAGTTTCGGAAATGTGTTGGAATTCTTTTTGAAGTACTTAAAAAAGAGTTTTTAAATTTTGAAAAATGAAGTAAACAGAGATTTTTTTTTTTTTTGTTTTTTTTTTTTTTGGATTTTGAAAAAGAATCCAAATGTTTCTTCAAAGAAAGTGAAACCAGGTAATAAAATTGAGACAAAACAAGTTTAAATTTAAAAAATCAAGTCATGGTTAACAGAGATGTAAATTTTCTGCTTGAACAATTTTCAAACATTCCAACCCTTGTACTAGTTACTTCTTAGATGCTGAAAAACAATTTTAATTTTTGAAACTTTTGTAATTCTCTCTTCTTCATCCATAACTCCCAATACCATACTCTTATATTCATATTAATGAAGTAAATGTTCATGGAGATCCTATGCTCAGAGATATTTACAATTTGCAAATCAAAGTTTCATCTTTACAATTGAACATTGTAGCTTCGAAAGCCCGACAGAATCAGACTTGGGAACTGGGAGGTGGCTGTGTTGTCAAAGGAGCAGCTGCACTATGCTGCCACAGACGCATTTGCTTCTTGGTATTTACACGAGGTATTATCAAATGACCCTTTTTTTCTCTTTTTATAGTAATTAATTCAGAATTTCATCAAATCTTCATAATCAAAAAAGATGTTTGAAATTACCTTTTCAATTACTTAAAAACTATTGTTCATTCCAAGAGTTCTTTATCATTATAACAATCTTAACCTCAGATCTTAAAAGGATTTCCACATGTTGAAAAAGTAGCAGATTCAGAGCCAATAGCGTGA

mRNA sequence

ATGGAATTTCCGGGCAGTAGCAACAGTGGCCATTCTTCGACGGAGCTCTCTGCGTGCTCCTCATCTTTTCCGTCGTGGTCGTTCTCCTCATCGGAGTTTGATCAGCCGTTTACAGAGCAACAACTCCAGGCAATCGAGGCCATTGAAGCTGCTTATCAATCCACCAGTGCCAAAAAACGACGACCAAATTCCACACTCGACGAAAACGGGGTCTCATGCGTTTCTCCCAACACCGGCCGTCGACTGCCTCGTTCCATTTTTTCTCAGCAGTCTCCACATTTTTCTCCCCTGTCATCCTGTCGAGTAAACTCGAAGATGAGGTTTCCTGCATTGAGTTATGGTGGCCGAATTATTTACAGTAGAACTGTAAGTGAGGTCGATAGAGCGTCCAGGGAGCTAGCGAAGAAAATCAACTCCACGAGGAAAGGAATGGAGCAAATTACTATTGGGTTCGACATCGAGTGGAGACCGTCATTTAAAAGAGGTGTTCCACCCGGAAAAGCAGCAGTTATGCAACTGTGCCTGGAAAATTCTGAATGCCATGTGATGCATATCATTCATTCGGGAATACCTCAAAGTTTGCAGGCTCTTCTAGAGGATGACACACTCAACAAGTTGGGATTGCCAGTGATGCTTCAAAAGTTTTTTAAAGATTATAACGTGTCTATGAAACAATTGGACGAAATTTCGGATCTAGCTAATCAAAAGCTTGCTGGAGTTCCCAAAAAATGGGGTCTTCGGGCTCTAACTGAGACGCTTATATCTAAAGAGCTTCGAAAGCCCGACAGAATCAGACTTGGGAACTGGGAGGTGGCTGTGTTGTCAAAGGAGCAGCTGCACTATGCTGCCACAGACGCATTTGCTTCTTGGTATTTACACGAGATCTTAAAAGGATTTCCACATGTTGAAAAAGTAGCAGATTCAGAGCCAATAGCGTGA

Coding sequence (CDS)

ATGGAATTTCCGGGCAGTAGCAACAGTGGCCATTCTTCGACGGAGCTCTCTGCGTGCTCCTCATCTTTTCCGTCGTGGTCGTTCTCCTCATCGGAGTTTGATCAGCCGTTTACAGAGCAACAACTCCAGGCAATCGAGGCCATTGAAGCTGCTTATCAATCCACCAGTGCCAAAAAACGACGACCAAATTCCACACTCGACGAAAACGGGGTCTCATGCGTTTCTCCCAACACCGGCCGTCGACTGCCTCGTTCCATTTTTTCTCAGCAGTCTCCACATTTTTCTCCCCTGTCATCCTGTCGAGTAAACTCGAAGATGAGGTTTCCTGCATTGAGTTATGGTGGCCGAATTATTTACAGTAGAACTGTAAGTGAGGTCGATAGAGCGTCCAGGGAGCTAGCGAAGAAAATCAACTCCACGAGGAAAGGAATGGAGCAAATTACTATTGGGTTCGACATCGAGTGGAGACCGTCATTTAAAAGAGGTGTTCCACCCGGAAAAGCAGCAGTTATGCAACTGTGCCTGGAAAATTCTGAATGCCATGTGATGCATATCATTCATTCGGGAATACCTCAAAGTTTGCAGGCTCTTCTAGAGGATGACACACTCAACAAGTTGGGATTGCCAGTGATGCTTCAAAAGTTTTTTAAAGATTATAACGTGTCTATGAAACAATTGGACGAAATTTCGGATCTAGCTAATCAAAAGCTTGCTGGAGTTCCCAAAAAATGGGGTCTTCGGGCTCTAACTGAGACGCTTATATCTAAAGAGCTTCGAAAGCCCGACAGAATCAGACTTGGGAACTGGGAGGTGGCTGTGTTGTCAAAGGAGCAGCTGCACTATGCTGCCACAGACGCATTTGCTTCTTGGTATTTACACGAGATCTTAAAAGGATTTCCACATGTTGAAAAAGTAGCAGATTCAGAGCCAATAGCGTGA

Protein sequence

MEFPGSSNSGHSSTELSACSSSFPSWSFSSSEFDQPFTEQQLQAIEAIEAAYQSTSAKKRRPNSTLDENGVSCVSPNTGRRLPRSIFSQQSPHFSPLSSCRVNSKMRFPALSYGGRIIYSRTVSEVDRASRELAKKINSTRKGMEQITIGFDIEWRPSFKRGVPPGKAAVMQLCLENSECHVMHIIHSGIPQSLQALLEDDTLNKLGLPVMLQKFFKDYNVSMKQLDEISDLANQKLAGVPKKWGLRALTETLISKELRKPDRIRLGNWEVAVLSKEQLHYAATDAFASWYLHEILKGFPHVEKVADSEPIA
Homology
BLAST of IVF0018306 vs. ExPASy Swiss-Prot
Match: Q84LH3 (Werner Syndrome-like exonuclease OS=Arabidopsis thaliana OX=3702 GN=WEX PE=1 SV=1)

HSP 1 Score: 263.8 bits (673), Expect = 2.4e-69
Identity = 142/284 (50.00%), Postives = 192/284 (67.61%), Query Frame = 0

Query: 29  SSSEFDQPFTEQQLQAIEAIEAAY----QSTSAKKRRPNSTL------DENGVSCVSPNT 88
           SS+  D  FTE++L AI+AIEA+Y     S+S+    P           E   + +  N 
Sbjct: 3   SSNWIDDAFTEEELLAIDAIEASYNFSRSSSSSSSAAPTVQATTSVHGHEEDPNQIPNNI 62

Query: 89  GRRLPRSIFSQQSPHFSPLSSCRVNSKMRFPALSYGGRIIYSRTVSEVDRASRELAKKIN 148
            R+LPRSI S  S    PLS CR  +   FPA+ +GGRI+YS+T +EVD+ + +L K ++
Sbjct: 63  RRQLPRSITSSTSYKRFPLSRCRARN---FPAMRFGGRILYSKTATEVDKRAMQLIKVLD 122

Query: 149 STRKGMEQITIGFDIEWRPSFKRGVPPGKAAVMQLCLENSECHVMHIIHSGIPQSLQALL 208
           + R       +G DIEWRPSF++GV PGK A +Q+C++++ C VMHI HSGIPQSLQ L+
Sbjct: 123 TKRDESGIAFVGLDIEWRPSFRKGVLPGKVATVQICVDSNYCDVMHIFHSGIPQSLQHLI 182

Query: 209 EDDTLNKLGLPV--MLQKFFKDYNVSMKQLDEISDLANQKLAGVPKKWGLRALTETLISK 268
           ED TL K+G+ +     K F DY VS+K ++++SDLANQK+ G  KKWGL +LTETL+ K
Sbjct: 183 EDSTLVKVGIGIDGDSVKLFHDYGVSIKDVEDLSDLANQKIGG-DKKWGLASLTETLVCK 242

Query: 269 ELRKPDRIRLGNWEVAVLSKEQLHYAATDAFASWYLHEILKGFP 301
           EL KP+RIRLGNWE   LSK+QL YAATDA+ASW+L+++LK  P
Sbjct: 243 ELLKPNRIRLGNWEFYPLSKQQLQYAATDAYASWHLYKVLKDLP 282

BLAST of IVF0018306 vs. ExPASy Swiss-Prot
Match: O93530 (Werner syndrome ATP-dependent helicase homolog OS=Xenopus laevis OX=8355 GN=wrn PE=2 SV=1)

HSP 1 Score: 122.1 bits (305), Expect = 1.1e-26
Identity = 66/205 (32.20%), Postives = 117/205 (57.07%), Query Frame = 0

Query: 98  SSCRVN-SKMRFPALSYGGRIIYSRTVSEVDRASRELAKKINSTRKGMEQITIGFDIEWR 157
           S C+ N  +   P + + G I+YS   ++    S ++   +      +E+  +GFDIEW 
Sbjct: 27  SFCKKNILEDNLPFMKFNGSIVYSYESNDCSLLSEDIRSSL------LEEDVLGFDIEWP 86

Query: 158 PSFKRGVPPGKAAVMQLCLENSECHVMHII-HSGIPQSLQALLEDDTLNKLGLPVM--LQ 217
           P + +G   GK A++Q+C+   +C++ HI   +G P+ L+ LLED+++ K+G+ +     
Sbjct: 87  PVYTKG-KTGKVALIQVCVSEKKCYLFHISPMAGFPKGLKRLLEDESVRKVGVGIEGDQW 146

Query: 218 KFFKDYNVSMKQLDEISDLANQKLAGVPKKWGLRALTETLISKELRKPDRIRLGNWEVAV 277
           K   DY + +K   E+S++ANQKL    +KW    L + L  ++L K    R  NW++ +
Sbjct: 147 KLMSDYELKLKGFIELSEMANQKLR-CKEKWTFNGLIKHLFKEQLYKRKSYRCSNWDIFL 206

Query: 278 LSKEQLHYAATDAFASWYLHEILKG 299
           L+++Q  YAATDA+A   +++ L+G
Sbjct: 207 LTEDQKLYAATDAYAGLLIYKKLEG 223

BLAST of IVF0018306 vs. ExPASy Swiss-Prot
Match: Q14191 (Werner syndrome ATP-dependent helicase OS=Homo sapiens OX=9606 GN=WRN PE=1 SV=2)

HSP 1 Score: 117.9 bits (294), Expect = 2.1e-25
Identity = 63/192 (32.81%), Postives = 110/192 (57.29%), Query Frame = 0

Query: 109 PALSYGGRIIYSRTVSEVDRASRELAKKINSTRKGMEQITIGFDIEWRPSFKRGVPPGKA 168
           P L + G I+YS   S+    S +++  ++      +   +GFD+EW P + RG   GK 
Sbjct: 45  PFLEFTGSIVYSYDASDCSFLSEDISMSLS------DGDVVGFDMEWPPLYNRG-KLGKV 104

Query: 169 AVMQLCLENSECHVMHIIHSGI-PQSLQALLEDDTLNKLGLPVM--LQKFFKDYNVSMKQ 228
           A++QLC+  S+C++ H+    + PQ L+ LLE+  + K G+ +     K  +D+++ +K 
Sbjct: 105 ALIQLCVSESKCYLFHVSSMSVFPQGLKMLLENKAVKKAGVGIEGDQWKLLRDFDIKLKN 164

Query: 229 LDEISDLANQKLAGVPKKWGLRALTETLISKELRKPDRIRLGNWEVAVLSKEQLHYAATD 288
             E++D+AN+KL    + W L +L + L+ K+L K   IR  NW    L+++Q  YAATD
Sbjct: 165 FVELTDVANKKLK-CTETWSLNSLVKHLLGKQLLKDKSIRCSNWSKFPLTEDQKLYAATD 224

Query: 289 AFASWYLHEILK 298
           A+A + ++  L+
Sbjct: 225 AYAGFIIYRNLE 228

BLAST of IVF0018306 vs. ExPASy Swiss-Prot
Match: O09053 (Werner syndrome ATP-dependent helicase homolog OS=Mus musculus OX=10090 GN=Wrn PE=1 SV=3)

HSP 1 Score: 113.6 bits (283), Expect = 4.0e-24
Identity = 62/191 (32.46%), Postives = 109/191 (57.07%), Query Frame = 0

Query: 109 PALSYGGRIIYSRTVSEVDRASRELAKKINSTRKGMEQITIGFDIEWRPSFKRGVPPGKA 168
           P L + G I+YS   S+    S +++ +++      +   +GFD+EW P +K G    + 
Sbjct: 39  PFLEFPGSIVYSYEASDCSFLSEDISMRLS------DGDVVGFDMEWPPIYKPG-KRSRV 98

Query: 169 AVMQLCLENSECHVMHIIHSGI-PQSLQALLEDDTLNKLGLPVM--LQKFFKDYNVSMKQ 228
           AV+QLC+  S+C++ HI    + PQ L+ LLE+ ++ K G+ +     K  +D++V ++ 
Sbjct: 99  AVIQLCVSESKCYLFHISSMSVFPQGLKMLLENKSIKKAGVGIEGDQWKLLRDFDVKLES 158

Query: 229 LDEISDLANQKLAGVPKKWGLRALTETLISKELRKPDRIRLGNWEVAVLSKEQLHYAATD 288
             E++D+AN+KL    + W L  L + ++ K+L K   IR  NW    L+++Q  YAATD
Sbjct: 159 FVELTDVANEKLK-CAETWSLNGLVKHVLGKQLLKDKSIRCSNWSNFPLTEDQKLYAATD 218

Query: 289 AFASWYLHEIL 297
           A+A   +++ L
Sbjct: 219 AYAGLIIYQKL 221

BLAST of IVF0018306 vs. ExPASy Swiss-Prot
Match: Q9VGN7 (Exonuclease 3'-5' domain-containing protein 2 OS=Drosophila melanogaster OX=7227 GN=Exd2 PE=2 SV=2)

HSP 1 Score: 65.9 bits (159), Expect = 9.5e-10
Identity = 49/151 (32.45%), Postives = 73/151 (48.34%), Query Frame = 0

Query: 149 IGFDIEWRPSFKRGVPPGKAAVMQLCLENSECHVMHIIH-SGIPQSLQALLEDDTLNKLG 208
           +GFD EW      G      A++QL      C +  + H   IPQ L+ LLEDD++ K+G
Sbjct: 80  LGFDCEW---ITVGGSRRPVALLQLSSHRGLCALFRLCHMKQIPQDLRELLEDDSVIKVG 139

Query: 209 LPVM--LQKFFKDYNVSMKQLDEISDLANQKLAGVPKKWGLRALTETLISKELRKPDRIR 268
           +       K   DY V +    ++  L    +AG  K  GL  L++T ++  L K  R+ 
Sbjct: 140 VAPQEDAMKLSHDYGVGVASTLDLRFLC--VMAG-HKPEGLGKLSKTHLNYTLDKHWRLA 199

Query: 269 LGNWEVAVLSKEQLHYAATDAFASWYLHEIL 297
             NWE   L  +QL YAA DA  +  +++ L
Sbjct: 200 CSNWEAKTLEPKQLDYAANDALMAVAIYQKL 224

BLAST of IVF0018306 vs. ExPASy TrEMBL
Match: A0A1S3AWQ6 (Werner Syndrome-like exonuclease OS=Cucumis melo OX=3656 GN=LOC103483463 PE=4 SV=1)

HSP 1 Score: 599.7 bits (1545), Expect = 6.8e-168
Identity = 304/314 (96.82%), Postives = 306/314 (97.45%), Query Frame = 0

Query: 1   MEFPGSSNSGHSSTELSACSSSFPSWSFSSSEFDQPFTEQQLQAIEAIEAAYQSTSAKKR 60
           MEFPGSSNSGHSSTELSACSSSFPSWSFSSSEFDQPFTEQQLQAIEAIEAAYQSTSAKKR
Sbjct: 1   MEFPGSSNSGHSSTELSACSSSFPSWSFSSSEFDQPFTEQQLQAIEAIEAAYQSTSAKKR 60

Query: 61  RPNSTLDENGVSCVSPNTGRRLPRSIFSQQSPHFSPLSSCRVNSKMRFPALSYGGRIIYS 120
           RPNSTLDENGVSCVSPNTGRRLPRSIFSQQSPHFSPLSSCRVNSKMRFPALSYGGRIIYS
Sbjct: 61  RPNSTLDENGVSCVSPNTGRRLPRSIFSQQSPHFSPLSSCRVNSKMRFPALSYGGRIIYS 120

Query: 121 RTVSEVDRASRELAKKINSTRKGMEQITIGFDIEWRPSFKRGVPPGKAAVMQLCLENSEC 180
           RTVSEVDRASRELAKKINSTRKGMEQITIGFDIEWRPSFKRGVPPGKAAVMQLCLENSEC
Sbjct: 121 RTVSEVDRASRELAKKINSTRKGMEQITIGFDIEWRPSFKRGVPPGKAAVMQLCLENSEC 180

Query: 181 HVMHIIHSGIPQSLQALLEDDTLNKLGLPVM--LQKFFKDYNVSMKQLDEISDLANQKLA 240
           HVMHIIHSGIPQSLQALLEDDTLNK G+ +     K FKDYNVSMKQLDEISDLANQKLA
Sbjct: 181 HVMHIIHSGIPQSLQALLEDDTLNKAGVGIASDASKVFKDYNVSMKQLDEISDLANQKLA 240

Query: 241 GVPKKWGLRALTETLISKELRKPDRIRLGNWEVAVLSKEQLHYAATDAFASWYLHEILKG 300
           GVPKKWGLRALTETLISKELRKPDRIRLGNWEVAVLSKEQLHYAATDAFASWYLHEILKG
Sbjct: 241 GVPKKWGLRALTETLISKELRKPDRIRLGNWEVAVLSKEQLHYAATDAFASWYLHEILKG 300

Query: 301 FPHVEKVADSEPIA 313
           FPHVEKVADSEPIA
Sbjct: 301 FPHVEKVADSEPIA 314

BLAST of IVF0018306 vs. ExPASy TrEMBL
Match: A0A5A7TZU7 (Werner Syndrome-like exonuclease OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G002920 PE=4 SV=1)

HSP 1 Score: 587.8 bits (1514), Expect = 2.7e-164
Identity = 298/308 (96.75%), Postives = 300/308 (97.40%), Query Frame = 0

Query: 7   SNSGHSSTELSACSSSFPSWSFSSSEFDQPFTEQQLQAIEAIEAAYQSTSAKKRRPNSTL 66
           SNSGHSSTELSACSSSFPSWSFSSSEFDQPFTEQQLQAIEAIEAAYQSTSAKKRRPNSTL
Sbjct: 32  SNSGHSSTELSACSSSFPSWSFSSSEFDQPFTEQQLQAIEAIEAAYQSTSAKKRRPNSTL 91

Query: 67  DENGVSCVSPNTGRRLPRSIFSQQSPHFSPLSSCRVNSKMRFPALSYGGRIIYSRTVSEV 126
           DENGVSCVSPNTGRRLPRSIFSQQSPHFSPLSSCRVNSKMRFPALSYGGRIIYSRTVSEV
Sbjct: 92  DENGVSCVSPNTGRRLPRSIFSQQSPHFSPLSSCRVNSKMRFPALSYGGRIIYSRTVSEV 151

Query: 127 DRASRELAKKINSTRKGMEQITIGFDIEWRPSFKRGVPPGKAAVMQLCLENSECHVMHII 186
           DRASRELAKKINSTRKGMEQITIGFDIEWRPSFKRGVPPGKAAVMQLCLENSECHVMHII
Sbjct: 152 DRASRELAKKINSTRKGMEQITIGFDIEWRPSFKRGVPPGKAAVMQLCLENSECHVMHII 211

Query: 187 HSGIPQSLQALLEDDTLNKLGLPVM--LQKFFKDYNVSMKQLDEISDLANQKLAGVPKKW 246
           HSGIPQSLQALLEDDTLNK G+ +     K FKDYNVSMKQLDEISDLANQKLAGVPKKW
Sbjct: 212 HSGIPQSLQALLEDDTLNKAGVGIASDASKVFKDYNVSMKQLDEISDLANQKLAGVPKKW 271

Query: 247 GLRALTETLISKELRKPDRIRLGNWEVAVLSKEQLHYAATDAFASWYLHEILKGFPHVEK 306
           GLRALTETLISKELRKPDRIRLGNWEVAVLSKEQLHYAATDAFASWYLHEILKGFPHVEK
Sbjct: 272 GLRALTETLISKELRKPDRIRLGNWEVAVLSKEQLHYAATDAFASWYLHEILKGFPHVEK 331

Query: 307 VADSEPIA 313
           VADSEPIA
Sbjct: 332 VADSEPIA 339

BLAST of IVF0018306 vs. ExPASy TrEMBL
Match: A0A0A0L9B9 (3'-5' exonuclease domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G129680 PE=4 SV=1)

HSP 1 Score: 553.9 bits (1426), Expect = 4.3e-154
Identity = 282/314 (89.81%), Postives = 294/314 (93.63%), Query Frame = 0

Query: 1   MEFPGSSNSGHSSTELSACSSSFPSWSFSSSEFDQPFTEQQLQAIEAIEAAYQSTSAKKR 60
           MEF GSSNS HSSTELSACSSSFP WSFS+SEFDQPFTEQQLQAIEAIEAAYQSTSAKKR
Sbjct: 1   MEFRGSSNSSHSSTELSACSSSFPPWSFSASEFDQPFTEQQLQAIEAIEAAYQSTSAKKR 60

Query: 61  RPNSTLDENGVSCVSPNTGRRLPRSIFSQQSPHFSPLSSCRVNSKMRFPALSYGGRIIYS 120
           RPNST D N VSCVSP+TGRRLPRSIFS QSP FSPLS CRVNSKMRFPAL+YGGRIIYS
Sbjct: 61  RPNSTPDGNEVSCVSPDTGRRLPRSIFSHQSPRFSPLSPCRVNSKMRFPALNYGGRIIYS 120

Query: 121 RTVSEVDRASRELAKKINSTRKGMEQITIGFDIEWRPSFKRGVPPGKAAVMQLCLENSEC 180
           RTVSEVDRASRELAKKINSTRK M+QITIGFDIEWRPSFKRGVPPGKAAVMQLCLENSEC
Sbjct: 121 RTVSEVDRASRELAKKINSTRKAMDQITIGFDIEWRPSFKRGVPPGKAAVMQLCLENSEC 180

Query: 181 HVMHIIHSGIPQSLQALLEDDTLNKLGLPVM--LQKFFKDYNVSMKQLDEISDLANQKLA 240
           HVMHIIHSGIPQSLQALLEDDTL+K G+ +     K FK+YNVS+K L+EISDLANQKLA
Sbjct: 181 HVMHIIHSGIPQSLQALLEDDTLSKAGVGIASDASKVFKEYNVSVKPLNEISDLANQKLA 240

Query: 241 GVPKKWGLRALTETLISKELRKPDRIRLGNWEVAVLSKEQLHYAATDAFASWYLHEILKG 300
           GVPKKWGLRALTETLISKEL+KPDRIRLGNWEVAVLSK+QL YAATDAFASWYLHEILKG
Sbjct: 241 GVPKKWGLRALTETLISKELQKPDRIRLGNWEVAVLSKDQLQYAATDAFASWYLHEILKG 300

Query: 301 FPHVEKVADSEPIA 313
           FPHVEKVADSEPIA
Sbjct: 301 FPHVEKVADSEPIA 314

BLAST of IVF0018306 vs. ExPASy TrEMBL
Match: A0A6J1G5L3 (Werner Syndrome-like exonuclease OS=Cucurbita moschata OX=3662 GN=LOC111451092 PE=4 SV=1)

HSP 1 Score: 474.6 bits (1220), Expect = 3.3e-130
Identity = 244/311 (78.46%), Postives = 269/311 (86.50%), Query Frame = 0

Query: 1   MEFPGSSNSGHSSTELSACSSSFPSWSFSSSEFDQPFTEQQLQAIEAIEAAYQSTSAKKR 60
           MEFP SSN   +STELS CSS   S+SFS SEFDQPFTEQQLQAIEAIEAAYQ+T+AK+R
Sbjct: 1   MEFPCSSNRAGASTELSVCSSPSSSYSFSLSEFDQPFTEQQLQAIEAIEAAYQATTAKRR 60

Query: 61  RPNSTLDENGVSCVSPNTGRRLPRSIFSQQSPHFSPLSSCRVNSKMRFPALSYGGRIIYS 120
           R      EN V CVSPN+GRRLPRSI S QSPH SPLS CRV SKMRFPAL +GGRI+YS
Sbjct: 61  R------ENEVLCVSPNSGRRLPRSISSLQSPHSSPLSPCRVTSKMRFPALKFGGRIVYS 120

Query: 121 RTVSEVDRASRELAKKINSTRKGMEQITIGFDIEWRPSFKRGVPPGKAAVMQLCLENSEC 180
           RT SEVDRASRELAKKINS RKGM+QITIGFDIEW+P+F+RGVPPGKAAVMQLCL++S+C
Sbjct: 121 RTASEVDRASRELAKKINSMRKGMDQITIGFDIEWKPTFRRGVPPGKAAVMQLCLDHSQC 180

Query: 181 HVMHIIHSGIPQSLQALLEDDTLNKLGLPVM--LQKFFKDYNVSMKQLDEISDLANQKLA 240
           HVMHIIHSGIP+SLQALLEDDTLNK G+ ++    K FKDYNVS+K +DEIS+LANQKLA
Sbjct: 181 HVMHIIHSGIPRSLQALLEDDTLNKAGVGIVNDAMKVFKDYNVSIKAVDEISNLANQKLA 240

Query: 241 GVPKKWGLRALTETLISKELRKPDRIRLGNWEVAVLSKEQLHYAATDAFASWYLHEILKG 300
           GVPKKW L +LTE LISKEL KP +IRLGNWEVAVLSKEQLHYAATDAFASWYLHEILKG
Sbjct: 241 GVPKKWSLGSLTEALISKELPKPSKIRLGNWEVAVLSKEQLHYAATDAFASWYLHEILKG 300

Query: 301 FPHVEKVADSE 310
           FP  +  ADSE
Sbjct: 301 FPDPQAAADSE 305

BLAST of IVF0018306 vs. ExPASy TrEMBL
Match: A0A6J1IC67 (Werner Syndrome-like exonuclease OS=Cucurbita maxima OX=3661 GN=LOC111473470 PE=4 SV=1)

HSP 1 Score: 461.8 bits (1187), Expect = 2.2e-126
Identity = 239/312 (76.60%), Postives = 268/312 (85.90%), Query Frame = 0

Query: 1   MEFPGSSNSGHSSTELSACSSSFPSWSFSSSEFDQPFTEQQLQAIEAIEAAYQSTSAKKR 60
           MEFP SSN   +STELS CSS   S+SFS SEFDQPFTEQQLQAIEAIEA YQ+T+AK+R
Sbjct: 1   MEFPCSSNRAGASTELSVCSSPSSSYSFSVSEFDQPFTEQQLQAIEAIEAEYQATTAKRR 60

Query: 61  RPNSTLDENGVSCVSPNTGRRLPRSIFSQQSPHFSPLSSCRVNSKMRFPALSYGGRIIYS 120
           R      EN V CVSPN+GRRLPRSI S QSPH SPLS CRV S MRFPAL +GGRI+YS
Sbjct: 61  R------ENEVLCVSPNSGRRLPRSISSLQSPHSSPLSPCRVTSMMRFPALKFGGRIVYS 120

Query: 121 RTVSEVDRASRELAKKINSTRKGMEQITIGFDIEWRPSFKRGVPPGKAAVMQLCLENSEC 180
           RT SEVDRASRELAKKINS RKGM+QITIGFDIEW+P+F+RGVPPGKAAVMQLCL++S+C
Sbjct: 121 RTASEVDRASRELAKKINSMRKGMDQITIGFDIEWKPTFRRGVPPGKAAVMQLCLDHSQC 180

Query: 181 HVMHIIHSGIPQSLQALLEDDTLNKLGLPVM--LQKFFKDYNVSMKQLDEISDLANQKL- 240
           HVMHIIHSGIP+SLQALLED+TL+K G+ ++    K FKDYNVS++ +DEIS+LANQKL 
Sbjct: 181 HVMHIIHSGIPRSLQALLEDNTLSKAGVGIVNDAMKVFKDYNVSIRAVDEISNLANQKLA 240

Query: 241 AGVPKKWGLRALTETLISKELRKPDRIRLGNWEVAVLSKEQLHYAATDAFASWYLHEILK 300
           AGVPKKW L +LTE LISKEL KP +IRLGNWEVA+LSKEQLHYAATDAFASWYLHEILK
Sbjct: 241 AGVPKKWSLGSLTEALISKELPKPSKIRLGNWEVAMLSKEQLHYAATDAFASWYLHEILK 300

Query: 301 GFPHVEKVADSE 310
           GFP  +K ADSE
Sbjct: 301 GFPDPQKAADSE 306

BLAST of IVF0018306 vs. NCBI nr
Match: XP_008438323.1 (PREDICTED: Werner Syndrome-like exonuclease [Cucumis melo])

HSP 1 Score: 599 bits (1545), Expect = 1.20e-215
Identity = 304/314 (96.82%), Postives = 306/314 (97.45%), Query Frame = 0

Query: 1   MEFPGSSNSGHSSTELSACSSSFPSWSFSSSEFDQPFTEQQLQAIEAIEAAYQSTSAKKR 60
           MEFPGSSNSGHSSTELSACSSSFPSWSFSSSEFDQPFTEQQLQAIEAIEAAYQSTSAKKR
Sbjct: 1   MEFPGSSNSGHSSTELSACSSSFPSWSFSSSEFDQPFTEQQLQAIEAIEAAYQSTSAKKR 60

Query: 61  RPNSTLDENGVSCVSPNTGRRLPRSIFSQQSPHFSPLSSCRVNSKMRFPALSYGGRIIYS 120
           RPNSTLDENGVSCVSPNTGRRLPRSIFSQQSPHFSPLSSCRVNSKMRFPALSYGGRIIYS
Sbjct: 61  RPNSTLDENGVSCVSPNTGRRLPRSIFSQQSPHFSPLSSCRVNSKMRFPALSYGGRIIYS 120

Query: 121 RTVSEVDRASRELAKKINSTRKGMEQITIGFDIEWRPSFKRGVPPGKAAVMQLCLENSEC 180
           RTVSEVDRASRELAKKINSTRKGMEQITIGFDIEWRPSFKRGVPPGKAAVMQLCLENSEC
Sbjct: 121 RTVSEVDRASRELAKKINSTRKGMEQITIGFDIEWRPSFKRGVPPGKAAVMQLCLENSEC 180

Query: 181 HVMHIIHSGIPQSLQALLEDDTLNKLGLPVM--LQKFFKDYNVSMKQLDEISDLANQKLA 240
           HVMHIIHSGIPQSLQALLEDDTLNK G+ +     K FKDYNVSMKQLDEISDLANQKLA
Sbjct: 181 HVMHIIHSGIPQSLQALLEDDTLNKAGVGIASDASKVFKDYNVSMKQLDEISDLANQKLA 240

Query: 241 GVPKKWGLRALTETLISKELRKPDRIRLGNWEVAVLSKEQLHYAATDAFASWYLHEILKG 300
           GVPKKWGLRALTETLISKELRKPDRIRLGNWEVAVLSKEQLHYAATDAFASWYLHEILKG
Sbjct: 241 GVPKKWGLRALTETLISKELRKPDRIRLGNWEVAVLSKEQLHYAATDAFASWYLHEILKG 300

Query: 301 FPHVEKVADSEPIA 312
           FPHVEKVADSEPIA
Sbjct: 301 FPHVEKVADSEPIA 314

BLAST of IVF0018306 vs. NCBI nr
Match: KAA0049133.1 (Werner Syndrome-like exonuclease [Cucumis melo var. makuwa] >TYK17428.1 Werner Syndrome-like exonuclease [Cucumis melo var. makuwa])

HSP 1 Score: 587 bits (1513), Expect = 2.27e-210
Identity = 298/308 (96.75%), Postives = 300/308 (97.40%), Query Frame = 0

Query: 7   SNSGHSSTELSACSSSFPSWSFSSSEFDQPFTEQQLQAIEAIEAAYQSTSAKKRRPNSTL 66
           SNSGHSSTELSACSSSFPSWSFSSSEFDQPFTEQQLQAIEAIEAAYQSTSAKKRRPNSTL
Sbjct: 32  SNSGHSSTELSACSSSFPSWSFSSSEFDQPFTEQQLQAIEAIEAAYQSTSAKKRRPNSTL 91

Query: 67  DENGVSCVSPNTGRRLPRSIFSQQSPHFSPLSSCRVNSKMRFPALSYGGRIIYSRTVSEV 126
           DENGVSCVSPNTGRRLPRSIFSQQSPHFSPLSSCRVNSKMRFPALSYGGRIIYSRTVSEV
Sbjct: 92  DENGVSCVSPNTGRRLPRSIFSQQSPHFSPLSSCRVNSKMRFPALSYGGRIIYSRTVSEV 151

Query: 127 DRASRELAKKINSTRKGMEQITIGFDIEWRPSFKRGVPPGKAAVMQLCLENSECHVMHII 186
           DRASRELAKKINSTRKGMEQITIGFDIEWRPSFKRGVPPGKAAVMQLCLENSECHVMHII
Sbjct: 152 DRASRELAKKINSTRKGMEQITIGFDIEWRPSFKRGVPPGKAAVMQLCLENSECHVMHII 211

Query: 187 HSGIPQSLQALLEDDTLNKLGLPVM--LQKFFKDYNVSMKQLDEISDLANQKLAGVPKKW 246
           HSGIPQSLQALLEDDTLNK G+ +     K FKDYNVSMKQLDEISDLANQKLAGVPKKW
Sbjct: 212 HSGIPQSLQALLEDDTLNKAGVGIASDASKVFKDYNVSMKQLDEISDLANQKLAGVPKKW 271

Query: 247 GLRALTETLISKELRKPDRIRLGNWEVAVLSKEQLHYAATDAFASWYLHEILKGFPHVEK 306
           GLRALTETLISKELRKPDRIRLGNWEVAVLSKEQLHYAATDAFASWYLHEILKGFPHVEK
Sbjct: 272 GLRALTETLISKELRKPDRIRLGNWEVAVLSKEQLHYAATDAFASWYLHEILKGFPHVEK 331

Query: 307 VADSEPIA 312
           VADSEPIA
Sbjct: 332 VADSEPIA 339

BLAST of IVF0018306 vs. NCBI nr
Match: XP_004133981.1 (Werner Syndrome-like exonuclease isoform X1 [Cucumis sativus] >KGN56711.1 hypothetical protein Csa_010757 [Cucumis sativus])

HSP 1 Score: 553 bits (1426), Expect = 1.63e-197
Identity = 282/314 (89.81%), Postives = 294/314 (93.63%), Query Frame = 0

Query: 1   MEFPGSSNSGHSSTELSACSSSFPSWSFSSSEFDQPFTEQQLQAIEAIEAAYQSTSAKKR 60
           MEF GSSNS HSSTELSACSSSFP WSFS+SEFDQPFTEQQLQAIEAIEAAYQSTSAKKR
Sbjct: 1   MEFRGSSNSSHSSTELSACSSSFPPWSFSASEFDQPFTEQQLQAIEAIEAAYQSTSAKKR 60

Query: 61  RPNSTLDENGVSCVSPNTGRRLPRSIFSQQSPHFSPLSSCRVNSKMRFPALSYGGRIIYS 120
           RPNST D N VSCVSP+TGRRLPRSIFS QSP FSPLS CRVNSKMRFPAL+YGGRIIYS
Sbjct: 61  RPNSTPDGNEVSCVSPDTGRRLPRSIFSHQSPRFSPLSPCRVNSKMRFPALNYGGRIIYS 120

Query: 121 RTVSEVDRASRELAKKINSTRKGMEQITIGFDIEWRPSFKRGVPPGKAAVMQLCLENSEC 180
           RTVSEVDRASRELAKKINSTRK M+QITIGFDIEWRPSFKRGVPPGKAAVMQLCLENSEC
Sbjct: 121 RTVSEVDRASRELAKKINSTRKAMDQITIGFDIEWRPSFKRGVPPGKAAVMQLCLENSEC 180

Query: 181 HVMHIIHSGIPQSLQALLEDDTLNKLGLPVM--LQKFFKDYNVSMKQLDEISDLANQKLA 240
           HVMHIIHSGIPQSLQALLEDDTL+K G+ +     K FK+YNVS+K L+EISDLANQKLA
Sbjct: 181 HVMHIIHSGIPQSLQALLEDDTLSKAGVGIASDASKVFKEYNVSVKPLNEISDLANQKLA 240

Query: 241 GVPKKWGLRALTETLISKELRKPDRIRLGNWEVAVLSKEQLHYAATDAFASWYLHEILKG 300
           GVPKKWGLRALTETLISKEL+KPDRIRLGNWEVAVLSK+QL YAATDAFASWYLHEILKG
Sbjct: 241 GVPKKWGLRALTETLISKELQKPDRIRLGNWEVAVLSKDQLQYAATDAFASWYLHEILKG 300

Query: 301 FPHVEKVADSEPIA 312
           FPHVEKVADSEPIA
Sbjct: 301 FPHVEKVADSEPIA 314

BLAST of IVF0018306 vs. NCBI nr
Match: XP_031738583.1 (Werner Syndrome-like exonuclease isoform X2 [Cucumis sativus])

HSP 1 Score: 520 bits (1338), Expect = 2.49e-184
Identity = 265/297 (89.23%), Postives = 277/297 (93.27%), Query Frame = 0

Query: 1   MEFPGSSNSGHSSTELSACSSSFPSWSFSSSEFDQPFTEQQLQAIEAIEAAYQSTSAKKR 60
           MEF GSSNS HSSTELSACSSSFP WSFS+SEFDQPFTEQQLQAIEAIEAAYQSTSAKKR
Sbjct: 1   MEFRGSSNSSHSSTELSACSSSFPPWSFSASEFDQPFTEQQLQAIEAIEAAYQSTSAKKR 60

Query: 61  RPNSTLDENGVSCVSPNTGRRLPRSIFSQQSPHFSPLSSCRVNSKMRFPALSYGGRIIYS 120
           RPNST D N VSCVSP+TGRRLPRSIFS QSP FSPLS CRVNSKMRFPAL+YGGRIIYS
Sbjct: 61  RPNSTPDGNEVSCVSPDTGRRLPRSIFSHQSPRFSPLSPCRVNSKMRFPALNYGGRIIYS 120

Query: 121 RTVSEVDRASRELAKKINSTRKGMEQITIGFDIEWRPSFKRGVPPGKAAVMQLCLENSEC 180
           RTVSEVDRASRELAKKINSTRK M+QITIGFDIEWRPSFKRGVPPGKAAVMQLCLENSEC
Sbjct: 121 RTVSEVDRASRELAKKINSTRKAMDQITIGFDIEWRPSFKRGVPPGKAAVMQLCLENSEC 180

Query: 181 HVMHIIHSGIPQSLQALLEDDTLNKLGLPVM--LQKFFKDYNVSMKQLDEISDLANQKLA 240
           HVMHIIHSGIPQSLQALLEDDTL+K G+ +     K FK+YNVS+K L+EISDLANQKLA
Sbjct: 181 HVMHIIHSGIPQSLQALLEDDTLSKAGVGIASDASKVFKEYNVSVKPLNEISDLANQKLA 240

Query: 241 GVPKKWGLRALTETLISKELRKPDRIRLGNWEVAVLSKEQLHYAATDAFASWYLHEI 295
           GVPKKWGLRALTETLISKEL+KPDRIRLGNWEVAVLSK+QL YAATDAFASWYLHEI
Sbjct: 241 GVPKKWGLRALTETLISKELQKPDRIRLGNWEVAVLSKDQLQYAATDAFASWYLHEI 297

BLAST of IVF0018306 vs. NCBI nr
Match: XP_038897657.1 (Werner Syndrome-like exonuclease [Benincasa hispida])

HSP 1 Score: 508 bits (1307), Expect = 2.11e-179
Identity = 258/313 (82.43%), Postives = 281/313 (89.78%), Query Frame = 0

Query: 1   MEFPGSSNSGHSSTELSACSSSFPSWSFSSSEFDQPFTEQQLQAIEAIEAAYQSTSAKKR 60
           MEF  SSN GHSST+LS CSSS  SWSFS+SEFDQPFTEQQLQ IEAIEAAYQSTSAKKR
Sbjct: 1   MEFHCSSNGGHSSTKLSVCSSS-SSWSFSASEFDQPFTEQQLQEIEAIEAAYQSTSAKKR 60

Query: 61  RPNSTLDENGVSCVSPNTGRRLPRSIFSQQSPHFSPLSSCRVNSKMRFPALSYGGRIIYS 120
           RPNST DEN VSCVSP+TGRRLPRSI S QSPH SPLS CRVNSKMRFPAL++GGRIIYS
Sbjct: 61  RPNSTPDENEVSCVSPDTGRRLPRSILSHQSPHSSPLSPCRVNSKMRFPALNFGGRIIYS 120

Query: 121 RTVSEVDRASRELAKKINSTRKGMEQITIGFDIEWRPSFKRGVPPGKAAVMQLCLENSEC 180
           RT SEVDRASRELAKKINS RKGM+QITIGFDIEW+P+F+RGVPPGKAAVMQLCL+NS+C
Sbjct: 121 RTWSEVDRASRELAKKINSMRKGMDQITIGFDIEWKPTFRRGVPPGKAAVMQLCLDNSQC 180

Query: 181 HVMHIIHSGIPQSLQALLEDDTLNKLGLPVM--LQKFFKDYNVSMKQLDEISDLANQKLA 240
           HVMHIIHSGIPQSLQALLEDDTL K G+ ++    K  KDYNVS+K +DEISD+ANQKLA
Sbjct: 181 HVMHIIHSGIPQSLQALLEDDTLTKAGVGIVNDATKVLKDYNVSIKAVDEISDIANQKLA 240

Query: 241 GVPKKWGLRALTETLISKELRKPDRIRLGNWEVAVLSKEQLHYAATDAFASWYLHEILKG 300
           G P+KW L +LTETLISKEL+KP++IRLGNWEVAVLS EQL YAATDAFASWYLHEILKG
Sbjct: 241 GFPRKWSLGSLTETLISKELQKPNKIRLGNWEVAVLSTEQLQYAATDAFASWYLHEILKG 300

Query: 301 FPHVEKVADSEPI 311
           FPH +KVAD EPI
Sbjct: 301 FPHAQKVADPEPI 312

BLAST of IVF0018306 vs. TAIR 10
Match: AT4G13870.2 (Werner syndrome-like exonuclease )

HSP 1 Score: 263.8 bits (673), Expect = 1.7e-70
Identity = 142/284 (50.00%), Postives = 192/284 (67.61%), Query Frame = 0

Query: 29  SSSEFDQPFTEQQLQAIEAIEAAY----QSTSAKKRRPNSTL------DENGVSCVSPNT 88
           SS+  D  FTE++L AI+AIEA+Y     S+S+    P           E   + +  N 
Sbjct: 3   SSNWIDDAFTEEELLAIDAIEASYNFSRSSSSSSSAAPTVQATTSVHGHEEDPNQIPNNI 62

Query: 89  GRRLPRSIFSQQSPHFSPLSSCRVNSKMRFPALSYGGRIIYSRTVSEVDRASRELAKKIN 148
            R+LPRSI S  S    PLS CR  +   FPA+ +GGRI+YS+T +EVD+ + +L K ++
Sbjct: 63  RRQLPRSITSSTSYKRFPLSRCRARN---FPAMRFGGRILYSKTATEVDKRAMQLIKVLD 122

Query: 149 STRKGMEQITIGFDIEWRPSFKRGVPPGKAAVMQLCLENSECHVMHIIHSGIPQSLQALL 208
           + R       +G DIEWRPSF++GV PGK A +Q+C++++ C VMHI HSGIPQSLQ L+
Sbjct: 123 TKRDESGIAFVGLDIEWRPSFRKGVLPGKVATVQICVDSNYCDVMHIFHSGIPQSLQHLI 182

Query: 209 EDDTLNKLGLPV--MLQKFFKDYNVSMKQLDEISDLANQKLAGVPKKWGLRALTETLISK 268
           ED TL K+G+ +     K F DY VS+K ++++SDLANQK+ G  KKWGL +LTETL+ K
Sbjct: 183 EDSTLVKVGIGIDGDSVKLFHDYGVSIKDVEDLSDLANQKIGG-DKKWGLASLTETLVCK 242

Query: 269 ELRKPDRIRLGNWEVAVLSKEQLHYAATDAFASWYLHEILKGFP 301
           EL KP+RIRLGNWE   LSK+QL YAATDA+ASW+L+++LK  P
Sbjct: 243 ELLKPNRIRLGNWEFYPLSKQQLQYAATDAYASWHLYKVLKDLP 282

BLAST of IVF0018306 vs. TAIR 10
Match: AT4G13870.1 (Werner syndrome-like exonuclease )

HSP 1 Score: 256.9 bits (655), Expect = 2.1e-68
Identity = 140/280 (50.00%), Postives = 189/280 (67.50%), Query Frame = 0

Query: 29  SSSEFDQPFTEQQLQAIEAIEAAY----QSTSAKKRRPNSTL------DENGVSCVSPNT 88
           SS+  D  FTE++L AI+AIEA+Y     S+S+    P           E   + +  N 
Sbjct: 3   SSNWIDDAFTEEELLAIDAIEASYNFSRSSSSSSSAAPTVQATTSVHGHEEDPNQIPNNI 62

Query: 89  GRRLPRSIFSQQSPHFSPLSSCRVNSKMRFPALSYGGRIIYSRTVSEVDRASRELAKKIN 148
            R+LPRSI S  S    PLS CR  +   FPA+ +GGRI+YS+T +EVD+ + +L K ++
Sbjct: 63  RRQLPRSITSSTSYKRFPLSRCRARN---FPAMRFGGRILYSKTATEVDKRAMQLIKVLD 122

Query: 149 STRKGMEQITIGFDIEWRPSFKRGVPPGKAAVMQLCLENSECHVMHIIHSGIPQSLQALL 208
           + R       +G DIEWRPSF++GV PGK A +Q+C++++ C VMHI HSGIPQSLQ L+
Sbjct: 123 TKRDESGIAFVGLDIEWRPSFRKGVLPGKVATVQICVDSNYCDVMHIFHSGIPQSLQHLI 182

Query: 209 EDDTLNKLGLPV--MLQKFFKDYNVSMKQLDEISDLANQKLAGVPKKWGLRALTETLISK 268
           ED TL K+G+ +     K F DY VS+K ++++SDLANQK+ G  KKWGL +LTETL+ K
Sbjct: 183 EDSTLVKVGIGIDGDSVKLFHDYGVSIKDVEDLSDLANQKIGG-DKKWGLASLTETLVCK 242

Query: 269 ELRKPDRIRLGNWEVAVLSKEQLHYAATDAFASWYLHEIL 297
           EL KP+RIRLGNWE   LSK+QL YAATDA+ASW+L++ L
Sbjct: 243 ELLKPNRIRLGNWEFYPLSKQQLQYAATDAYASWHLYKDL 278

BLAST of IVF0018306 vs. TAIR 10
Match: AT4G13885.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 71.2 bits (173), Expect = 1.6e-12
Identity = 31/43 (72.09%), Postives = 37/43 (86.05%), Query Frame = 0

Query: 258 LRKPDRIRLGNWEVAVLSKEQLHYAATDAFASWYLHEILKGFP 301
           L KP+RIRLGNWEV  LSK+QL YAATDA+ASW+LH++LK  P
Sbjct: 215 LLKPNRIRLGNWEVHPLSKQQLQYAATDAYASWHLHQVLKDLP 257

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q84LH32.4e-6950.00Werner Syndrome-like exonuclease OS=Arabidopsis thaliana OX=3702 GN=WEX PE=1 SV=... [more]
O935301.1e-2632.20Werner syndrome ATP-dependent helicase homolog OS=Xenopus laevis OX=8355 GN=wrn ... [more]
Q141912.1e-2532.81Werner syndrome ATP-dependent helicase OS=Homo sapiens OX=9606 GN=WRN PE=1 SV=2[more]
O090534.0e-2432.46Werner syndrome ATP-dependent helicase homolog OS=Mus musculus OX=10090 GN=Wrn P... [more]
Q9VGN79.5e-1032.45Exonuclease 3'-5' domain-containing protein 2 OS=Drosophila melanogaster OX=7227... [more]
Match NameE-valueIdentityDescription
A0A1S3AWQ66.8e-16896.82Werner Syndrome-like exonuclease OS=Cucumis melo OX=3656 GN=LOC103483463 PE=4 SV... [more]
A0A5A7TZU72.7e-16496.75Werner Syndrome-like exonuclease OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A0A0L9B94.3e-15489.813'-5' exonuclease domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G... [more]
A0A6J1G5L33.3e-13078.46Werner Syndrome-like exonuclease OS=Cucurbita moschata OX=3662 GN=LOC111451092 P... [more]
A0A6J1IC672.2e-12676.60Werner Syndrome-like exonuclease OS=Cucurbita maxima OX=3661 GN=LOC111473470 PE=... [more]
Match NameE-valueIdentityDescription
XP_008438323.11.20e-21596.82PREDICTED: Werner Syndrome-like exonuclease [Cucumis melo][more]
KAA0049133.12.27e-21096.75Werner Syndrome-like exonuclease [Cucumis melo var. makuwa] >TYK17428.1 Werner S... [more]
XP_004133981.11.63e-19789.81Werner Syndrome-like exonuclease isoform X1 [Cucumis sativus] >KGN56711.1 hypoth... [more]
XP_031738583.12.49e-18489.23Werner Syndrome-like exonuclease isoform X2 [Cucumis sativus][more]
XP_038897657.12.11e-17982.43Werner Syndrome-like exonuclease [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT4G13870.21.7e-7050.00Werner syndrome-like exonuclease [more]
AT4G13870.12.1e-6850.00Werner syndrome-like exonuclease [more]
AT4G13885.11.6e-1272.09Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR0025623'-5' exonuclease domainSMARTSM0047435exoneu6coord: 117..301
e-value: 2.3E-12
score: 57.1
IPR0025623'-5' exonuclease domainPFAMPF01612DNA_pol_A_exo1coord: 134..297
e-value: 2.8E-10
score: 40.2
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 100..299
e-value: 3.0E-45
score: 155.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 53..77
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..31
NoneNo IPR availablePANTHERPTHR136203-5 EXONUCLEASEcoord: 46..304
NoneNo IPR availablePANTHERPTHR13620:SF45POLYNUCLEOTIDYL TRANSFERASE, RIBONUCLEASE H-LIKE SUPERFAMILY PROTEIN-RELATEDcoord: 46..304
NoneNo IPR availableCDDcd06141WRN_exocoord: 121..297
e-value: 4.91721E-48
score: 156.97
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 73..298

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
IVF0018306.1IVF0018306.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0090305 nucleic acid phosphodiester bond hydrolysis
biological_process GO:0006139 nucleobase-containing compound metabolic process
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005634 nucleus
molecular_function GO:0008408 3'-5' exonuclease activity
molecular_function GO:0003676 nucleic acid binding