IVF0018138 (gene) Melon (IVF77) v1

Overview


Name	IVF0018138
Type	gene
Organism	Cucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
Description	acyl-CoA--sterol O-acyltransferase 1-like
Location	chr04: 22707940 .. 22709082 (+)
RNA-Seq Expression	IVF0018138
Synteny	IVF0018138

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAGAGAAAAAAAAAAATGGAGGGAGAAATCGTAAACTTAACCAAGGTATGGTTAACCGTATGTATCTCTCTCTCTTACTGCCACGCCACCGGCAAACTCCTCAATCCCGGCGTCGCCCGCTTCCTCGCCATCGCCCCTATCGTCCTCCTCTTCTTCCTCCTCCCTCTCCAACTTACTTCCATCCACTTCGGCGGCGCCACCTGCTTCTTCGTCACTTGGCTCGCCACCTTCAAGCTCCTCCTCTTCTCTGCCGGCGACGGCCCTCTCTCCACTACCCCCCCGCTTTCTCTCCGCCGATTCATAGCCATATCTTGTTTGCCCATCAAGATCCTGGAACACCCCTCGGAGAAATCGGCCCCTACCAATAATCGAGCGCAAACCCTTCACGAAAAACCCAAATCGCTTCTCAACTACTCGATTAGAGCCCTGATCGTTGTCCTGATGGTGAAATTGTACGATTACTCTGAGTTTTTACACCCAAAACTTGTTCTGTTCATTTATAGTTGGCACGTTTACCTTCTCCTTGAGATTATTCTGGCGATTACCCAGTTTTTTGGTCGTAACCTGCTCGGAATCGAGCTGGAACCGCAATTTCGGGATCCGTACTATTCGACTTCGCTTCAGGATTTTTGGGGAAGGCGATGGAATCTGATGGCGACGAATATTCTTCAGCCGGCGGTTTACAAGCCGACGGTGAAGATGGCTGCTAAGGTAATAGGGAGGATGTGGGCGCCGTTGCCGGGGGTGATGGCGACGTTTTTGGTGTCGGCAATGATGCACGAACTGATATTTTATTATTTGGGGAGAATGAGGCCGGACTGGGAGATCACGTGGTTCTTTGTTATACATGGAATTGCTTTGACGGTGGAGATTATGATGAAGAAGATGTTGCCTGAGAAGCGGAGGTTACCGGCGAAGGTGTCGGTGCCGGTGACTCTAGTTTTCATATTCTCGACGGCAATTTGGTTGTTTTTTCCGCAGTTCGTGAGACTGAAGTTGGACGTGAGAGCGTTTGAAGAATACGCGGCGTTGGCCAATTTTGTGAAGAAAGTCGCTTTCTCGTTGGTCGGTTGAGTTTTCTTTTACTTTCTTTCGGGGTCGGTCACGGCCATTGTTTGGTGGTTATTAATTGTATTAGATCCG

mRNA sequence

AAGAGAAAAAAAAAAATGGAGGGAGAAATCGTAAACTTAACCAAGGTATGGTTAACCGTATGTATCTCTCTCTCTTACTGCCACGCCACCGGCAAACTCCTCAATCCCGGCGTCGCCCGCTTCCTCGCCATCGCCCCTATCGTCCTCCTCTTCTTCCTCCTCCCTCTCCAACTTACTTCCATCCACTTCGGCGGCGCCACCTGCTTCTTCGTCACTTGGCTCGCCACCTTCAAGCTCCTCCTCTTCTCTGCCGGCGACGGCCCTCTCTCCACTACCCCCCCGCTTTCTCTCCGCCGATTCATAGCCATATCTTGTTTGCCCATCAAGATCCTGGAACACCCCTCGGAGAAATCGGCCCCTACCAATAATCGAGCGCAAACCCTTCACGAAAAACCCAAATCGCTTCTCAACTACTCGATTAGAGCCCTGATCGTTGTCCTGATGGTGAAATTGTACGATTACTCTGAGTTTTTACACCCAAAACTTGTTCTGTTCATTTATAGTTGGCACGTTTACCTTCTCCTTGAGATTATTCTGGCGATTACCCAGTTTTTTGGTCGTAACCTGCTCGGAATCGAGCTGGAACCGCAATTTCGGGATCCGTACTATTCGACTTCGCTTCAGGATTTTTGGGGAAGGCGATGGAATCTGATGGCGACGAATATTCTTCAGCCGGCGGTTTACAAGCCGACGGTGAAGATGGCTGCTAAGGTAATAGGGAGGATGTGGGCGCCGTTGCCGGGGGTGATGGCGACGTTTTTGGTGTCGGCAATGATGCACGAACTGATATTTTATTATTTGGGGAGAATGAGGCCGGACTGGGAGATCACGTGGTTCTTTGTTATACATGGAATTGCTTTGACGGTGGAGATTATGATGAAGAAGATGTTGCCTGAGAAGCGGAGGTTACCGGCGAAGGTGTCGGTGCCGGTGACTCTAGTTTTCATATTCTCGACGGCAATTTGGTTGTTTTTTCCGCAGTTCGTGAGACTGAAGTTGGACGTGAGAGCGTTTGAAGAATACGCGGCGTTGGCCAATTTTGTGAAGAAAGTCGCTTTCTCGTTGGTCGGTTGAGTTTTCTTTTACTTTCTTTCGGGGTCGGTCACGGCCATTGTTTGGTGGTTATTAATTGTATTAGATCCG

Coding sequence (CDS)

ATGGAGGGAGAAATCGTAAACTTAACCAAGGTATGGTTAACCGTATGTATCTCTCTCTCTTACTGCCACGCCACCGGCAAACTCCTCAATCCCGGCGTCGCCCGCTTCCTCGCCATCGCCCCTATCGTCCTCCTCTTCTTCCTCCTCCCTCTCCAACTTACTTCCATCCACTTCGGCGGCGCCACCTGCTTCTTCGTCACTTGGCTCGCCACCTTCAAGCTCCTCCTCTTCTCTGCCGGCGACGGCCCTCTCTCCACTACCCCCCCGCTTTCTCTCCGCCGATTCATAGCCATATCTTGTTTGCCCATCAAGATCCTGGAACACCCCTCGGAGAAATCGGCCCCTACCAATAATCGAGCGCAAACCCTTCACGAAAAACCCAAATCGCTTCTCAACTACTCGATTAGAGCCCTGATCGTTGTCCTGATGGTGAAATTGTACGATTACTCTGAGTTTTTACACCCAAAACTTGTTCTGTTCATTTATAGTTGGCACGTTTACCTTCTCCTTGAGATTATTCTGGCGATTACCCAGTTTTTTGGTCGTAACCTGCTCGGAATCGAGCTGGAACCGCAATTTCGGGATCCGTACTATTCGACTTCGCTTCAGGATTTTTGGGGAAGGCGATGGAATCTGATGGCGACGAATATTCTTCAGCCGGCGGTTTACAAGCCGACGGTGAAGATGGCTGCTAAGGTAATAGGGAGGATGTGGGCGCCGTTGCCGGGGGTGATGGCGACGTTTTTGGTGTCGGCAATGATGCACGAACTGATATTTTATTATTTGGGGAGAATGAGGCCGGACTGGGAGATCACGTGGTTCTTTGTTATACATGGAATTGCTTTGACGGTGGAGATTATGATGAAGAAGATGTTGCCTGAGAAGCGGAGGTTACCGGCGAAGGTGTCGGTGCCGGTGACTCTAGTTTTCATATTCTCGACGGCAATTTGGTTGTTTTTTCCGCAGTTCGTGAGACTGAAGTTGGACGTGAGAGCGTTTGAAGAATACGCGGCGTTGGCCAATTTTGTGAAGAAAGTCGCTTTCTCGTTGGTCGGTTGA

Protein sequence

MEGEIVNLTKVWLTVCISLSYCHATGKLLNPGVARFLAIAPIVLLFFLLPLQLTSIHFGGATCFFVTWLATFKLLLFSAGDGPLSTTPPLSLRRFIAISCLPIKILEHPSEKSAPTNNRAQTLHEKPKSLLNYSIRALIVVLMVKLYDYSEFLHPKLVLFIYSWHVYLLLEIILAITQFFGRNLLGIELEPQFRDPYYSTSLQDFWGRRWNLMATNILQPAVYKPTVKMAAKVIGRMWAPLPGVMATFLVSAMMHELIFYYLGRMRPDWEITWFFVIHGIALTVEIMMKKMLPEKRRLPAKVSVPVTLVFIFSTAIWLFFPQFVRLKLDVRAFEEYAALANFVKKVAFSLVG

Homology

[Close]

BLAST of IVF0018138 vs. ExPASy Swiss-Prot
Match: Q9SV07 (Acyl-CoA--sterol O-acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=ASAT1 PE=1 SV=1)

HSP 1 Score: 295.0 bits (754), Expect = 1.1e-78
Identity = 160/344 (46.51%), Postives = 224/344 (65.12%), Query Frame = 0


Query: 5   IVNLTKVWLTVCISLSYCHATGKLLNPGVARFLAIAPIVLLFFLLPLQLTSIHFGGATCF 64
           + +  K W  V ISL Y     KL+  G+ R +   P+ L+FF++P  + S+H  G T F
Sbjct: 1   MASFIKAWGLVIISLCYTFFIAKLVPKGIKRLILFFPVFLIFFIVPFLIYSLHLLGITAF 60

Query: 65  FVTWLATFKLLLFSAGDGPLSTT-PPLSLRRFIAISCLPIKILEHPSEKSAPTNNRAQTL 124
           F+ WLA FKLLLF+ G GPLS+   PLSL  F+A+SCLPIKI   P     PT   +   
Sbjct: 61  FIAWLANFKLLLFALGRGPLSSNHKPLSLPIFLAVSCLPIKIQLSPK----PTKTHS--- 120

Query: 125 HE-KPKSLLNYSIRALIVVLMVKLYDYSEFLHPKLVLFIYSWHVYLLLEIILAITQFFGR 184
           HE   +  L Y+I+A+ VVL++K Y+YS  L  K+VL +Y+ H+Y  LEIILA T    R
Sbjct: 121 HEGSTEGPLIYTIKAVFVVLIIKAYEYSTKLPEKVVLTLYAIHIYFALEIILAATAAAVR 180

Query: 185 NLLGIELEPQFRDPYYSTSLQDFWGRRWNLMATNILQPAVYKPTVKMAAKVIGRMWAPLP 244
            +  +ELEPQF  PY +TSLQDFWGRRWNLM T IL+P VY+P++++ + V+G  ++ + 
Sbjct: 181 AMSDLELEPQFNKPYLATSLQDFWGRRWNLMVTGILRPTVYEPSLQLFS-VLGPNYSQIL 240

Query: 245 GVMATFLVSAMMHELIFYYLGRMRPDWEITWFFVIHGIALTVEIMMKKMLPEKRRLPAKV 304
               TF+VS +MHELIF+Y+GR+RPDW++ WFF+I+G   TVEI +KK +  + R P  +
Sbjct: 241 AAFGTFVVSGIMHELIFFYMGRLRPDWKMMWFFLINGFCTTVEIAIKKTINGRWRFPKAI 300

Query: 305 SVPVTLVFIFSTAIWLFFPQFVRLKLDVRAFEEYAALANFVKKV 347
           S  +TL F+  TA+WLF P+F R  +  +A +EYAA+  F  +V
Sbjct: 301 SQVLTLTFVMVTALWLFLPEFNRCNIVEKALDEYAAIGAFAVEV 336

[Close]

BLAST of IVF0018138 vs. ExPASy Swiss-Prot
Match: Q9FJ76 (Probable long-chain-alcohol O-fatty-acyltransferase 5 OS=Arabidopsis thaliana OX=3702 GN=AT5 PE=2 SV=1)

HSP 1 Score: 254.6 bits (649), Expect = 1.6e-66
Identity = 142/347 (40.92%), Postives = 208/347 (59.94%), Query Frame = 0


Query: 1   MEGEIVNLTKVWLTVCISLSYCHATGKLLNPGVARFLAIAPIVLLFFLLPLQLTSIHFGG 60
           M+ E+ NL KVW++  IS+SYC+     +  G  RFL+++P++ LF +LPL  +S+H   
Sbjct: 1   MDEELKNLIKVWVSAIISISYCYYIPPRIKSGAPRFLSVSPVLALFLVLPLFFSSLHLSL 60

Query: 61  ATCFFVTWLATFKLLLFSAGDGPLSTTPPLSLRRFIAISCLPIKILEHPSEKSAPTNNRA 120
            T FF+TWLA FKL+LFS   GPL    P +  RF   +C PIK+ ++P  +        
Sbjct: 61  ITAFFLTWLANFKLILFSFDKGPLIPI-PTNFPRFFCFTCFPIKVQQNPKSQ-------- 120

Query: 121 QTLHEKPKSLLNYSIRALIVVLMVKLYDYSEFLHPKLVLFIYSWHVYLLLEIILAITQFF 180
              +  PK  L ++I+  I  +++ LY Y + L P ++L +Y  H+YL +EIIL   +  
Sbjct: 121 ---NHLPK--LVFAIKLAIFAVLLHLYSYRQNLSPTILLGLYFVHLYLEIEIILTFVKVV 180

Query: 181 GRNLLGIELEPQFRDPYYSTSLQDFWGRRWNLMATNILQPAVYKPTVKMAAKVIGRMWAP 240
               LG +LEPQ   PY +TSLQDFWGRRWNLM   IL+PAVY P  +++ + +   WA 
Sbjct: 181 VFISLGCDLEPQSNKPYLATSLQDFWGRRWNLMVPAILRPAVYAPMRRVSERKMSSGWAL 240

Query: 241 LPGVMATFLVSAMMHELIFYYLGRMRPDWEITWFFVIHGIALTVEIMMKK--MLPEKRRL 300
            PG++A F+VS ++HEL+F+YL R  P  E+T FFV+HG+   VE+ +KK   + ++ RL
Sbjct: 241 FPGILAAFIVSGLVHELLFFYLIREMPTGEVTLFFVLHGVCTAVELAVKKKTTVAQRWRL 300

Query: 301 PAKVSVPVTLVFIFSTAIWLFFPQFVRLKLDVRAFEEYAALANFVKK 346
              VS  +T+ F+F T  WLF PQ  R  +  R   E   L  F+K+
Sbjct: 301 SPGVSRVLTVGFVFVTGGWLFTPQLKRSGVMERFTSEAVLLVEFIKR 333

[Close]

BLAST of IVF0018138 vs. ExPASy Swiss-Prot
Match: Q9XGY6 (Long-chain-alcohol O-fatty-acyltransferase OS=Simmondsia chinensis OX=3999 PE=1 SV=1)

HSP 1 Score: 252.7 bits (644), Expect = 6.2e-66
Identity = 139/361 (38.50%), Postives = 211/361 (58.45%), Query Frame = 0


Query: 1   MEGEIVNLTKVWLTVCISLSYCHATGKLL-NPGVARFLAIAPIVLLFFLLPLQLTSIHFG 60
           +E E+   ++VW++   +  YC     +  + G  R L + P+VLLF  LPL+L+S H G
Sbjct: 3   VEKELKTFSEVWISAIAAACYCRFVPAVAPHGGALRLLLLLPVVLLFIFLPLRLSSFHLG 62

Query: 61  GATCFFVTWLATFKLLLFSAGDGPLSTTPPLSLRRFIAISCLPIKILEHPSEKSAPTNNR 120
           G T  ++ WLA FKLLLF+   GPLS  P LSL  FI+ + LPIK  + PS         
Sbjct: 63  GPTALYLVWLANFKLLLFAFHLGPLS-NPSLSLLHFISTTLLPIKFRDDPSND------- 122

Query: 121 AQTLHEKPKSLLNYSIRALIVVL--------MVKLYDYSEFLHPKLVLFIYSWHVYLLLE 180
               HEK K  L++  R +++ +        ++K+Y++ + L   ++  +Y +H YL  E
Sbjct: 123 ----HEKNKRTLSFEWRKVVLFVAKLVFFAGILKIYEFRKDLPHFVISVLYCFHFYLGTE 182

Query: 181 IILAITQFFGRNLLGIELEPQFRDPYYSTSLQDFWGRRWNLMATNILQPAVYKPTVKMAA 240
           I LA +    R  LG++L PQF +PY +TSLQDFWGRRWNLM ++IL    Y+P  ++ +
Sbjct: 183 ITLAASAVIARATLGLDLYPQFNEPYLATSLQDFWGRRWNLMVSDILGLTTYQPVRRVLS 242

Query: 241 KVIGRMWAPLPGVMATFLVSAMMHELIFYYLGRMRPDWEITWFFVIHGIALTVEIMMKKM 300
           + +   W     ++  F VS +MHE+ F+YL R RP WE+T FFV+HG+   VE+++KK 
Sbjct: 243 RWVRLRWEVAGAMLVAFTVSGLMHEVFFFYLTRARPSWEVTGFFVLHGVCTAVEMVVKKA 302

Query: 301 LPEKRRLPAKVSVPVTLVFIFSTAIWLFFPQFVRLKLDVRAFEEYAALANFVKKVAFSLV 353
           +  K RL  +VS  +T+ F+  T  WLF PQ VR  +D++  +EY  + N+ +K    L+
Sbjct: 303 VSGKVRLRREVSGALTVGFVMVTGGWLFLPQLVRHGVDLKTIDEYPVMFNYTQKKLMGLL 351

[Close]

BLAST of IVF0018138 vs. ExPASy Swiss-Prot
Match: Q9FJ75 (Probable long-chain-alcohol O-fatty-acyltransferase 4 OS=Arabidopsis thaliana OX=3702 GN=AT4 PE=2 SV=1)

HSP 1 Score: 251.1 bits (640), Expect = 1.8e-65
Identity = 141/351 (40.17%), Postives = 208/351 (59.26%), Query Frame = 0


Query: 1   MEGEIVNLTKVWLTVCISLSYCHATGKLLNPGVARFLAIAPIVLLFFLLPLQLTSIHFGG 60
           ME E+++L KVW+   IS+SYC+ T   +  GV R L++ P+ +LF +LPL ++S+HF G
Sbjct: 1   MEEELMSLIKVWVYAIISISYCYYTSTRIKSGVFRLLSVLPVCVLFLVLPLFVSSVHFSG 60

Query: 61  ATCFFVTWLATFKLLLFSAGDGPLSTTPPLSLRRFIAISCLPIKILEHPSEKSAPTNNRA 120
           +T FF++WLA FKL+LFS   GPL   P  +L RF+  +C PIK+ ++P  +        
Sbjct: 61  STAFFLSWLANFKLILFSFDQGPLFPVPS-NLSRFVCFTCFPIKLQQNPKPQ-------- 120

Query: 121 QTLHEKPKSLLNYSIRALIVVLMVKLYDYSEFLHPKLVLFIYSWHVYLLLEIILAITQFF 180
              ++ PK    ++++     +++ +Y+Y + + P ++L +YS H+YL  EI+LA  +  
Sbjct: 121 ---NQMPK--WGFAVKLAFFGVLLHMYEYKQHMSPTVLLVLYSLHIYLEYEILLAPLKVL 180

Query: 181 GRNLLGIELEPQFRDPYYSTSLQDFWGRRWNLMATNILQPAVYKPTVKMAAKVIGRMWAP 240
               L  +LEP F +PY STSLQDFWGRRWNLM   IL+PAVY P  +MA + +    A 
Sbjct: 181 LSISLWCDLEPHFNEPYLSTSLQDFWGRRWNLMVPAILRPAVYLPVRQMAGRKMNSDQAL 240

Query: 241 LPGVMATFLVSAMMHELIFYYLGRMRPDWEITWFFVIHGIALTVEIMMKK-MLPEKRRLP 300
             GV A+FLVS ++HELIF+Y  R  P  E+T FFV+HG+    E   K+  L  + ++ 
Sbjct: 241 FLGVFASFLVSGVVHELIFFYFTRESPTGEVTLFFVLHGVCTAAECAAKRTRLVRRWKVS 300

Query: 301 AKVSVPVTLVFIFSTAIWLFFPQFVRLKLDVRAFEEYAALANFVKKVAFSL 351
             VS  +T+ F+  T  WLFFP   R  +  R  +E      FVK   F L
Sbjct: 301 QMVSRLLTVGFVVMTGGWLFFPHLARSGMIERLADEAFLFIGFVKHKFFYL 337

[Close]

BLAST of IVF0018138 vs. ExPASy Swiss-Prot
Match: Q9FJ73 (Probable long-chain-alcohol O-fatty-acyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=AT2 PE=3 SV=1)

HSP 1 Score: 248.8 bits (634), Expect = 9.0e-65
Identity = 145/349 (41.55%), Postives = 209/349 (59.89%), Query Frame = 0


Query: 1   MEGEIVNLTKVWLTVCISLSYCHATGKLLNPGVARFLAIAPIVLLFFLLPLQLTSIHFGG 60
           ME E+ NL KVW++  IS+SYC+     ++ GV R L++ PI ++F LLPL  +S+HF  
Sbjct: 1   MEEELRNLIKVWISALISISYCYYISSKISKGVLRLLSLLPIFIIFLLLPLFFSSVHFCV 60

Query: 61  ATCFFVTWLATFKLLLFSAGDGPLSTTPPLSLRRFIAISCLPIKILEHPSEKSAPTNNRA 120
            + FF TWLA FKL LF+    PLS  P  +L RF   +C PIKI ++PS      +NR 
Sbjct: 61  ISGFFFTWLANFKLFLFAFDQEPLSPLPS-NLTRFFCFACFPIKINKNPS------SNR- 120

Query: 121 QTLHEKPKSLLNYSIRALIVVLMVKLY--DYSEFLHPKLVLFIYSWHVYLLLEIILAITQ 180
             +H KP S    + + LI   ++ +Y  +Y   L     L +++ HVYL  E+IL    
Sbjct: 121 --IHNKPMSKWVLAFKLLIFSFLLHVYRNNYDSGLSRFAFLALFTIHVYLEAELILVFVG 180

Query: 181 FFGRNLLGIELEPQFRDPYYSTSLQDFWGRRWNLMATNILQPAVYKPTVKMAAKVIGRMW 240
                LLG E+EP F DPY +TSLQ+FW RRWNLM   +L+PAV+ P  +  A ++G   
Sbjct: 181 ALMSMLLGCEMEPVFNDPYLATSLQEFWSRRWNLMVPAVLRPAVHIPVQRFCAPLLGLHR 240

Query: 241 APLPGVMATFLVSAMMHELIFYYLGRMRPDWEITWFFVIHGIALTVEIMMKKM--LPEKR 300
           A   G++ATF+VS +MHELI++Y+ R  P WE+T FF++HG+   +EI MK+M  LP  R
Sbjct: 241 AFYAGMLATFIVSGLMHELIYFYVIRKSPTWEVTCFFLLHGVVTCLEIAMKRMRWLPTPR 300

Query: 301 RLPAKVSVPVTLVFIFSTAIWLFFPQFVRLKLDVRAFEEYAALANFVKK 346
           R  + +++    VF+  TA WLF+PQ +R  +  R   E   + + VK+
Sbjct: 301 RAVSGLAI---TVFLLVTAGWLFYPQMLRNDVHKRVISECLLVIDVVKR 336

[Close]

BLAST of IVF0018138 vs. ExPASy TrEMBL
Match: A0A0A0L9S2 (MBOAT_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G684690 PE=3 SV=1)

HSP 1 Score: 653.7 bits (1685), Expect = 4.5e-184
Identity = 329/352 (93.47%), Postives = 340/352 (96.59%), Query Frame = 0


Query: 1   MEGEIVNLTKVWLTVCISLSYCHATGKLLNPGVARFLAIAPIVLLFFLLPLQLTSIHFGG 60
           ME EI+NLTKVWLTV ISL YCHAT KLLNPG ARFLAIAP+VL+FFLLPLQLTSIHFGG
Sbjct: 28  MEAEIINLTKVWLTVFISLGYCHATAKLLNPGPARFLAIAPVVLIFFLLPLQLTSIHFGG 87

Query: 61  ATCFFVTWLATFKLLLFSAGDGPLSTTPPLSLRRFIAISCLPIKILEHPSEKSAPTNNRA 120
           ATCFFVTWLATFKLLL +AGDGPLSTTPPL L RFIAISCLPIKILE+PSEKSAP NNRA
Sbjct: 88  ATCFFVTWLATFKLLLLTAGDGPLSTTPPLPLHRFIAISCLPIKILENPSEKSAPKNNRA 147

Query: 121 QTLHEKPKSLLNYSIRALIVVLMVKLYDYSEFLHPKLVLFIYSWHVYLLLEIILAITQFF 180
           +TL+EKPKS LNYSIRALIVVLMVKLYDYS F HPKLVLFIYSWHVYLLLEIILAITQFF
Sbjct: 148 ETLNEKPKSFLNYSIRALIVVLMVKLYDYSHFFHPKLVLFIYSWHVYLLLEIILAITQFF 207

Query: 181 GRNLLGIELEPQFRDPYYSTSLQDFWGRRWNLMATNILQPAVYKPTVKMAAKVIGRMWAP 240
           GRNLLGIELEPQFRDPYYSTSLQDFWGRRWNLMATNILQPAVYKPTVK+AA+VIGRMWAP
Sbjct: 208 GRNLLGIELEPQFRDPYYSTSLQDFWGRRWNLMATNILQPAVYKPTVKIAARVIGRMWAP 267

Query: 241 LPGVMATFLVSAMMHELIFYYLGRMRPDWEITWFFVIHGIALTVEIMMKKMLPEKRRLPA 300
           LPGVMATFLVSAMMHELIFYYLGRMRP+WEITWFFVIHGIALTVEIMMKKMLPEKRRLPA
Sbjct: 268 LPGVMATFLVSAMMHELIFYYLGRMRPNWEITWFFVIHGIALTVEIMMKKMLPEKRRLPA 327

Query: 301 KVSVPVTLVFIFSTAIWLFFPQFVRLKLDVRAFEEYAALANFVKKVAFSLVG 353
           KVSVPVTLVFIFSTAIWLFFPQFVRL+LDVRAFEEYAALANFVKKVAFSLVG
Sbjct: 328 KVSVPVTLVFIFSTAIWLFFPQFVRLRLDVRAFEEYAALANFVKKVAFSLVG 379

[Close]

BLAST of IVF0018138 vs. ExPASy TrEMBL
Match: A0A6J1J5C4 (acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111481453 PE=3 SV=1)

HSP 1 Score: 579.7 bits (1493), Expect = 8.2e-162
Identity = 280/351 (79.77%), Postives = 320/351 (91.17%), Query Frame = 0


Query: 1   MEGEIVNLTKVWLTVCISLSYCHATGKLLNPGVARFLAIAPIVLLFFLLPLQLTSIHFGG 60
           MEGEI+N T VWL VC+SL YCH  GK+L+PG  RFLAI+P+VLLFFLLPLQLTS+H GG
Sbjct: 1   MEGEIINFTMVWLIVCMSLCYCHTAGKILHPGAGRFLAISPVVLLFFLLPLQLTSVHLGG 60

Query: 61  ATCFFVTWLATFKLLLFSAGDGPLSTTPPLSLRRFIAISCLPIKILEHPSEKSAPTNNRA 120
           AT FF+ WLA FKLLLF+AG+GPLS TPPL LRRF+A+SCLPIKIL++PSEK +P N+  
Sbjct: 61  ATAFFIAWLANFKLLLFAAGNGPLSATPPLPLRRFLALSCLPIKILQNPSEKQSPKNHH- 120

Query: 121 QTLHEKPKSLLNYSIRALIVVLMVKLYDYSEFLHPKLVLFIYSWHVYLLLEIILAITQFF 180
           +TL+++PKS LNY I+ALIV LMVKLYDYSEF+HPK++LFIYSWHVY  LEIILAITQFF
Sbjct: 121 ETLNQEPKSFLNYWIKALIVALMVKLYDYSEFIHPKVILFIYSWHVYFFLEIILAITQFF 180

Query: 181 GRNLLGIELEPQFRDPYYSTSLQDFWGRRWNLMATNILQPAVYKPTVKMAAKVIGRMWAP 240
           GR LLGIELEPQF DPY+STSLQDFWGRRWNLMATNILQP VYKPTVK+AAKV+GR WAP
Sbjct: 181 GRTLLGIELEPQFHDPYFSTSLQDFWGRRWNLMATNILQPTVYKPTVKIAAKVVGRKWAP 240

Query: 241 LPGVMATFLVSAMMHELIFYYLGRMRPDWEITWFFVIHGIALTVEIMMKKMLPEKRRLPA 300
           LPGVMATFLVSA+MHELIFYYLGRM+P+WE+TWFFVIHG ALTVEI++KK+LPEKRRLPA
Sbjct: 241 LPGVMATFLVSAIMHELIFYYLGRMKPNWELTWFFVIHGFALTVEILVKKVLPEKRRLPA 300

Query: 301 KVSVPVTLVFIFSTAIWLFFPQFVRLKLDVRAFEEYAALANFVKKVAFSLV 352
            VSVPVTLVFIFSTAIWLFFPQF+RLK+DV+AFEEYAAL +F+KK+AF LV
Sbjct: 301 AVSVPVTLVFIFSTAIWLFFPQFMRLKIDVKAFEEYAALVSFMKKLAFPLV 350

[Close]

BLAST of IVF0018138 vs. ExPASy TrEMBL
Match: A0A6J1GBE5 (acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111452458 PE=3 SV=1)

HSP 1 Score: 578.9 bits (1491), Expect = 1.4e-161
Identity = 290/352 (82.39%), Postives = 316/352 (89.77%), Query Frame = 0


Query: 1   MEGEIVNLTKVWLTVCISLSYCHATGKLLNPGVARFLAIAPIVLLFFLLPLQLTSIHFGG 60
           MEGEI+N  KVWLTVCISL YCHATGKLL PG  RFLA+APIV+LFFLLPLQLTSIH GG
Sbjct: 1   MEGEILNFIKVWLTVCISLCYCHATGKLLAPGPGRFLAVAPIVVLFFLLPLQLTSIHLGG 60

Query: 61  ATCFFVTWLATFKLLLFSAGDGPLSTTPPLSLRRFIAISCLPIKILEHPSEKSAPTNNRA 120
           +T FF+ WLA FKLLLF+AGDGPLS  PPL LRRF+A+SCLPIKIL+  +EKS P NNR+
Sbjct: 61  STSFFIAWLANFKLLLFAAGDGPLSAEPPLPLRRFLALSCLPIKILQ--NEKSCPNNNRS 120

Query: 121 QTLHEKPKSLLNYSIRALIVVLMVKLYDYSEFLHPKLVLFIYSWHVYLLLEIILAITQFF 180
            T   KPKS LNYSI+A+IV LMVKLYDYSEF+HPK+VLFIYSWH+Y LLEIILAITQFF
Sbjct: 121 DTT-LKPKSFLNYSIKAVIVALMVKLYDYSEFIHPKVVLFIYSWHLYFLLEIILAITQFF 180

Query: 181 GRNLLGIELEPQFRDPYYSTSLQDFWGRRWNLMATNILQPAVYKPTVKMAAKVIGRMWAP 240
           GRN+LGIELEPQF+DPY+STSLQDFWGRRWNLMATNILQP VYKP+VK+AAKVIGR WAP
Sbjct: 181 GRNVLGIELEPQFQDPYFSTSLQDFWGRRWNLMATNILQPTVYKPSVKIAAKVIGRKWAP 240

Query: 241 LPGVMATFLVSAMMHELIFYYLGRMRPDWEITWFFVIHGIALTVEIMMKKMLPEKRRLPA 300
           LP VMATFLVSAMMHELIFYY+GRMRP+WE TWFFVIHG+ALTVEIM+KK+LPEK RLP 
Sbjct: 241 LPAVMATFLVSAMMHELIFYYMGRMRPNWECTWFFVIHGVALTVEIMVKKVLPEKWRLPP 300

Query: 301 KVSVPVTLVFIFSTAIWLFFPQFVRLKLDVRAFEEYAALANFVKKVAFSLVG 353
            VSVPVTL FIF TAIWLFFPQFVRLKLDVRAFEEYAAL  FVK VAFSLVG
Sbjct: 301 AVSVPVTLAFIFPTAIWLFFPQFVRLKLDVRAFEEYAALVTFVKNVAFSLVG 349

[Close]

BLAST of IVF0018138 vs. ExPASy TrEMBL
Match: A0A6J1KAA2 (acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111493091 PE=3 SV=1)

HSP 1 Score: 575.9 bits (1483), Expect = 1.2e-160
Identity = 287/352 (81.53%), Postives = 315/352 (89.49%), Query Frame = 0


Query: 1   MEGEIVNLTKVWLTVCISLSYCHATGKLLNPGVARFLAIAPIVLLFFLLPLQLTSIHFGG 60
           MEGEI+N  KVWLTVCISL YCHATGKL+ PG  RFLA+APIV+LFF LPLQLTSIH GG
Sbjct: 1   MEGEILNFIKVWLTVCISLCYCHATGKLIPPGAGRFLAVAPIVVLFFFLPLQLTSIHLGG 60

Query: 61  ATCFFVTWLATFKLLLFSAGDGPLSTTPPLSLRRFIAISCLPIKILEHPSEKSAPTNNRA 120
           +T FF+ WLA FKLLLF+AGDGPLS  PPL LRRF+A+SCLPIKIL+  +EKS P NNR+
Sbjct: 61  STSFFIAWLANFKLLLFTAGDGPLSAEPPLPLRRFLALSCLPIKILQ--NEKSCPNNNRS 120

Query: 121 QTLHEKPKSLLNYSIRALIVVLMVKLYDYSEFLHPKLVLFIYSWHVYLLLEIILAITQFF 180
            T   KPKS LNYSI+A+IV LMVKLYDYSEF+HPK+VLFIYSWH+Y LLEIILAITQFF
Sbjct: 121 DTT-LKPKSFLNYSIKAVIVALMVKLYDYSEFIHPKVVLFIYSWHLYFLLEIILAITQFF 180

Query: 181 GRNLLGIELEPQFRDPYYSTSLQDFWGRRWNLMATNILQPAVYKPTVKMAAKVIGRMWAP 240
           GRN+LGIELEPQF+DPY+STSLQDFWGRRWNLMATNILQP VYKP+VK+AAKVIGR WAP
Sbjct: 181 GRNVLGIELEPQFQDPYFSTSLQDFWGRRWNLMATNILQPTVYKPSVKIAAKVIGRKWAP 240

Query: 241 LPGVMATFLVSAMMHELIFYYLGRMRPDWEITWFFVIHGIALTVEIMMKKMLPEKRRLPA 300
           LP VMATFLVSAMMHELIF+Y+GRMRP+WE TWFFVIHG+ALTVEIM+KK+LPEK RLP 
Sbjct: 241 LPAVMATFLVSAMMHELIFFYMGRMRPNWECTWFFVIHGVALTVEIMVKKVLPEKWRLPP 300

Query: 301 KVSVPVTLVFIFSTAIWLFFPQFVRLKLDVRAFEEYAALANFVKKVAFSLVG 353
            VSVPVTL FIF TAIWLFFPQFVRLKLDVRAFEEYAAL  FVK VAFSLVG
Sbjct: 301 AVSVPVTLAFIFPTAIWLFFPQFVRLKLDVRAFEEYAALVTFVKNVAFSLVG 349

[Close]

BLAST of IVF0018138 vs. ExPASy TrEMBL
Match: A0A6J1F6Y6 (acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111441427 PE=3 SV=1)

HSP 1 Score: 575.1 bits (1481), Expect = 2.0e-160
Identity = 276/351 (78.63%), Postives = 318/351 (90.60%), Query Frame = 0


Query: 1   MEGEIVNLTKVWLTVCISLSYCHATGKLLNPGVARFLAIAPIVLLFFLLPLQLTSIHFGG 60
           MEGE++N   VWL VC+SL YCH  GK+L PG  RFLAIAP+V+LFFLLPLQLTS+H GG
Sbjct: 1   MEGEVINFIMVWLIVCMSLCYCHTAGKILPPGAGRFLAIAPVVVLFFLLPLQLTSVHLGG 60

Query: 61  ATCFFVTWLATFKLLLFSAGDGPLSTTPPLSLRRFIAISCLPIKILEHPSEKSAPTNNRA 120
           +T FF+ WLA FKLLLF+AG+GPLS TPPL LRRF+A+SCLPIKIL++PSEK +P N+  
Sbjct: 61  STAFFIAWLANFKLLLFTAGNGPLSATPPLPLRRFLALSCLPIKILQNPSEKQSPKNHH- 120

Query: 121 QTLHEKPKSLLNYSIRALIVVLMVKLYDYSEFLHPKLVLFIYSWHVYLLLEIILAITQFF 180
           +TL+++PKS LNY I+ALIV LMVKLYDYSEF+HPK++LFIYSWHVY  LEIILAITQFF
Sbjct: 121 ETLNQEPKSFLNYWIKALIVALMVKLYDYSEFIHPKVILFIYSWHVYFFLEIILAITQFF 180

Query: 181 GRNLLGIELEPQFRDPYYSTSLQDFWGRRWNLMATNILQPAVYKPTVKMAAKVIGRMWAP 240
           GR LLGIELEPQF DPY+STSLQDFWGRRWNLMATNILQP +YKPTVK+AAKV+GR WAP
Sbjct: 181 GRTLLGIELEPQFHDPYFSTSLQDFWGRRWNLMATNILQPTIYKPTVKIAAKVVGRKWAP 240

Query: 241 LPGVMATFLVSAMMHELIFYYLGRMRPDWEITWFFVIHGIALTVEIMMKKMLPEKRRLPA 300
           LPGVMATFLVSA+MHELIFYYLGRM+P+WE+TWFFVIHG ALTVEI++KK+LPEKRRLPA
Sbjct: 241 LPGVMATFLVSAIMHELIFYYLGRMKPNWELTWFFVIHGFALTVEILVKKVLPEKRRLPA 300

Query: 301 KVSVPVTLVFIFSTAIWLFFPQFVRLKLDVRAFEEYAALANFVKKVAFSLV 352
            VSVPVTLVFIFSTAIWLFFPQF+RLK+DV+AFEEYAAL +F+KK+AF LV
Sbjct: 301 AVSVPVTLVFIFSTAIWLFFPQFMRLKIDVKAFEEYAALVSFIKKLAFPLV 350

[Close]

BLAST of IVF0018138 vs. NCBI nr
Match: XP_004149740.1 (acyl-CoA--sterol O-acyltransferase 1 [Cucumis sativus])

HSP 1 Score: 651 bits (1680), Expect = 6.55e-235
Identity = 329/352 (93.47%), Postives = 340/352 (96.59%), Query Frame = 0


Query: 1   MEGEIVNLTKVWLTVCISLSYCHATGKLLNPGVARFLAIAPIVLLFFLLPLQLTSIHFGG 60
           ME EI+NLTKVWLTV ISL YCHAT KLLNPG ARFLAIAP+VL+FFLLPLQLTSIHFGG
Sbjct: 1   MEAEIINLTKVWLTVFISLGYCHATAKLLNPGPARFLAIAPVVLIFFLLPLQLTSIHFGG 60

Query: 61  ATCFFVTWLATFKLLLFSAGDGPLSTTPPLSLRRFIAISCLPIKILEHPSEKSAPTNNRA 120
           ATCFFVTWLATFKLLL +AGDGPLSTTPPL L RFIAISCLPIKILE+PSEKSAP NNRA
Sbjct: 61  ATCFFVTWLATFKLLLLTAGDGPLSTTPPLPLHRFIAISCLPIKILENPSEKSAPKNNRA 120

Query: 121 QTLHEKPKSLLNYSIRALIVVLMVKLYDYSEFLHPKLVLFIYSWHVYLLLEIILAITQFF 180
           +TL+EKPKS LNYSIRALIVVLMVKLYDYS F HPKLVLFIYSWHVYLLLEIILAITQFF
Sbjct: 121 ETLNEKPKSFLNYSIRALIVVLMVKLYDYSHFFHPKLVLFIYSWHVYLLLEIILAITQFF 180

Query: 181 GRNLLGIELEPQFRDPYYSTSLQDFWGRRWNLMATNILQPAVYKPTVKMAAKVIGRMWAP 240
           GRNLLGIELEPQFRDPYYSTSLQDFWGRRWNLMATNILQPAVYKPTVK+AA+VIGRMWAP
Sbjct: 181 GRNLLGIELEPQFRDPYYSTSLQDFWGRRWNLMATNILQPAVYKPTVKIAARVIGRMWAP 240

Query: 241 LPGVMATFLVSAMMHELIFYYLGRMRPDWEITWFFVIHGIALTVEIMMKKMLPEKRRLPA 300
           LPGVMATFLVSAMMHELIFYYLGRMRP+WEITWFFVIHGIALTVEIMMKKMLPEKRRLPA
Sbjct: 241 LPGVMATFLVSAMMHELIFYYLGRMRPNWEITWFFVIHGIALTVEIMMKKMLPEKRRLPA 300

Query: 301 KVSVPVTLVFIFSTAIWLFFPQFVRLKLDVRAFEEYAALANFVKKVAFSLVG 352
           KVSVPVTLVFIFSTAIWLFFPQFVRL+LDVRAFEEYAALANFVKKVAFSLVG
Sbjct: 301 KVSVPVTLVFIFSTAIWLFFPQFVRLRLDVRAFEEYAALANFVKKVAFSLVG 352

[Close]

BLAST of IVF0018138 vs. NCBI nr
Match: KGN58563.1 (hypothetical protein Csa_002202 [Cucumis sativus])

HSP 1 Score: 651 bits (1680), Expect = 1.79e-234
Identity = 329/352 (93.47%), Postives = 340/352 (96.59%), Query Frame = 0


Query: 1   MEGEIVNLTKVWLTVCISLSYCHATGKLLNPGVARFLAIAPIVLLFFLLPLQLTSIHFGG 60
           ME EI+NLTKVWLTV ISL YCHAT KLLNPG ARFLAIAP+VL+FFLLPLQLTSIHFGG
Sbjct: 28  MEAEIINLTKVWLTVFISLGYCHATAKLLNPGPARFLAIAPVVLIFFLLPLQLTSIHFGG 87

Query: 61  ATCFFVTWLATFKLLLFSAGDGPLSTTPPLSLRRFIAISCLPIKILEHPSEKSAPTNNRA 120
           ATCFFVTWLATFKLLL +AGDGPLSTTPPL L RFIAISCLPIKILE+PSEKSAP NNRA
Sbjct: 88  ATCFFVTWLATFKLLLLTAGDGPLSTTPPLPLHRFIAISCLPIKILENPSEKSAPKNNRA 147

Query: 121 QTLHEKPKSLLNYSIRALIVVLMVKLYDYSEFLHPKLVLFIYSWHVYLLLEIILAITQFF 180
           +TL+EKPKS LNYSIRALIVVLMVKLYDYS F HPKLVLFIYSWHVYLLLEIILAITQFF
Sbjct: 148 ETLNEKPKSFLNYSIRALIVVLMVKLYDYSHFFHPKLVLFIYSWHVYLLLEIILAITQFF 207

Query: 181 GRNLLGIELEPQFRDPYYSTSLQDFWGRRWNLMATNILQPAVYKPTVKMAAKVIGRMWAP 240
           GRNLLGIELEPQFRDPYYSTSLQDFWGRRWNLMATNILQPAVYKPTVK+AA+VIGRMWAP
Sbjct: 208 GRNLLGIELEPQFRDPYYSTSLQDFWGRRWNLMATNILQPAVYKPTVKIAARVIGRMWAP 267

Query: 241 LPGVMATFLVSAMMHELIFYYLGRMRPDWEITWFFVIHGIALTVEIMMKKMLPEKRRLPA 300
           LPGVMATFLVSAMMHELIFYYLGRMRP+WEITWFFVIHGIALTVEIMMKKMLPEKRRLPA
Sbjct: 268 LPGVMATFLVSAMMHELIFYYLGRMRPNWEITWFFVIHGIALTVEIMMKKMLPEKRRLPA 327

Query: 301 KVSVPVTLVFIFSTAIWLFFPQFVRLKLDVRAFEEYAALANFVKKVAFSLVG 352
           KVSVPVTLVFIFSTAIWLFFPQFVRL+LDVRAFEEYAALANFVKKVAFSLVG
Sbjct: 328 KVSVPVTLVFIFSTAIWLFFPQFVRLRLDVRAFEEYAALANFVKKVAFSLVG 379

[Close]

BLAST of IVF0018138 vs. NCBI nr
Match: XP_023527814.1 (acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 578 bits (1489), Expect = 7.92e-206
Identity = 280/351 (79.77%), Postives = 319/351 (90.88%), Query Frame = 0


Query: 1   MEGEIVNLTKVWLTVCISLSYCHATGKLLNPGVARFLAIAPIVLLFFLLPLQLTSIHFGG 60
           MEGEI+N T VWL VC+SL YCH  GK+L PG  RFLAIAP+VLLFFLLPLQLTS+H GG
Sbjct: 1   MEGEIINFTMVWLIVCMSLCYCHTAGKILPPGAGRFLAIAPVVLLFFLLPLQLTSVHLGG 60

Query: 61  ATCFFVTWLATFKLLLFSAGDGPLSTTPPLSLRRFIAISCLPIKILEHPSEKSAPTNNRA 120
           AT FF+ WLA FKLLLF+AG+GPLS TPPL LRRF+A+SCLPIKIL++PSEK +P N+  
Sbjct: 61  ATAFFIAWLANFKLLLFAAGNGPLSATPPLPLRRFLALSCLPIKILQNPSEKQSPKNHH- 120

Query: 121 QTLHEKPKSLLNYSIRALIVVLMVKLYDYSEFLHPKLVLFIYSWHVYLLLEIILAITQFF 180
           +TL+++PKS LNY I+ALIV LMVKLYDYSEF+HPK++LFIYSWHVY  LEIILAITQFF
Sbjct: 121 ETLNQEPKSFLNYWIKALIVALMVKLYDYSEFIHPKVILFIYSWHVYFFLEIILAITQFF 180

Query: 181 GRNLLGIELEPQFRDPYYSTSLQDFWGRRWNLMATNILQPAVYKPTVKMAAKVIGRMWAP 240
           GR LLGIELEPQF DPY+STSLQDFWGRRWNLMATNILQP +YKPTVK+AAKV+GR WAP
Sbjct: 181 GRTLLGIELEPQFHDPYFSTSLQDFWGRRWNLMATNILQPTIYKPTVKIAAKVVGRKWAP 240

Query: 241 LPGVMATFLVSAMMHELIFYYLGRMRPDWEITWFFVIHGIALTVEIMMKKMLPEKRRLPA 300
           LPGVMATFLVSA+MHELIFYYLGRM+P+WE+TWFFVIHG ALTVEI++KK+LPEKRRLPA
Sbjct: 241 LPGVMATFLVSAIMHELIFYYLGRMKPNWELTWFFVIHGFALTVEILVKKVLPEKRRLPA 300

Query: 301 KVSVPVTLVFIFSTAIWLFFPQFVRLKLDVRAFEEYAALANFVKKVAFSLV 351
            VSVPVTLVFIFSTAIWLFFPQF+RLK+DV+AFEEYAAL +F+KK+AF LV
Sbjct: 301 AVSVPVTLVFIFSTAIWLFFPQFMRLKIDVKAFEEYAALVSFIKKLAFPLV 350

[Close]

BLAST of IVF0018138 vs. NCBI nr
Match: XP_022982638.1 (acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita maxima])

HSP 1 Score: 577 bits (1488), Expect = 1.12e-205
Identity = 280/351 (79.77%), Postives = 320/351 (91.17%), Query Frame = 0


Query: 1   MEGEIVNLTKVWLTVCISLSYCHATGKLLNPGVARFLAIAPIVLLFFLLPLQLTSIHFGG 60
           MEGEI+N T VWL VC+SL YCH  GK+L+PG  RFLAI+P+VLLFFLLPLQLTS+H GG
Sbjct: 1   MEGEIINFTMVWLIVCMSLCYCHTAGKILHPGAGRFLAISPVVLLFFLLPLQLTSVHLGG 60

Query: 61  ATCFFVTWLATFKLLLFSAGDGPLSTTPPLSLRRFIAISCLPIKILEHPSEKSAPTNNRA 120
           AT FF+ WLA FKLLLF+AG+GPLS TPPL LRRF+A+SCLPIKIL++PSEK +P N+  
Sbjct: 61  ATAFFIAWLANFKLLLFAAGNGPLSATPPLPLRRFLALSCLPIKILQNPSEKQSPKNHH- 120

Query: 121 QTLHEKPKSLLNYSIRALIVVLMVKLYDYSEFLHPKLVLFIYSWHVYLLLEIILAITQFF 180
           +TL+++PKS LNY I+ALIV LMVKLYDYSEF+HPK++LFIYSWHVY  LEIILAITQFF
Sbjct: 121 ETLNQEPKSFLNYWIKALIVALMVKLYDYSEFIHPKVILFIYSWHVYFFLEIILAITQFF 180

Query: 181 GRNLLGIELEPQFRDPYYSTSLQDFWGRRWNLMATNILQPAVYKPTVKMAAKVIGRMWAP 240
           GR LLGIELEPQF DPY+STSLQDFWGRRWNLMATNILQP VYKPTVK+AAKV+GR WAP
Sbjct: 181 GRTLLGIELEPQFHDPYFSTSLQDFWGRRWNLMATNILQPTVYKPTVKIAAKVVGRKWAP 240

Query: 241 LPGVMATFLVSAMMHELIFYYLGRMRPDWEITWFFVIHGIALTVEIMMKKMLPEKRRLPA 300
           LPGVMATFLVSA+MHELIFYYLGRM+P+WE+TWFFVIHG ALTVEI++KK+LPEKRRLPA
Sbjct: 241 LPGVMATFLVSAIMHELIFYYLGRMKPNWELTWFFVIHGFALTVEILVKKVLPEKRRLPA 300

Query: 301 KVSVPVTLVFIFSTAIWLFFPQFVRLKLDVRAFEEYAALANFVKKVAFSLV 351
            VSVPVTLVFIFSTAIWLFFPQF+RLK+DV+AFEEYAAL +F+KK+AF LV
Sbjct: 301 AVSVPVTLVFIFSTAIWLFFPQFMRLKIDVKAFEEYAALVSFMKKLAFPLV 350

[Close]

BLAST of IVF0018138 vs. NCBI nr
Match: XP_022948970.1 (acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita moschata] >KAG6606895.1 Acyl-CoA--sterol O-acyltransferase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 576 bits (1485), Expect = 2.99e-205
Identity = 290/352 (82.39%), Postives = 316/352 (89.77%), Query Frame = 0


Query: 1   MEGEIVNLTKVWLTVCISLSYCHATGKLLNPGVARFLAIAPIVLLFFLLPLQLTSIHFGG 60
           MEGEI+N  KVWLTVCISL YCHATGKLL PG  RFLA+APIV+LFFLLPLQLTSIH GG
Sbjct: 1   MEGEILNFIKVWLTVCISLCYCHATGKLLAPGPGRFLAVAPIVVLFFLLPLQLTSIHLGG 60

Query: 61  ATCFFVTWLATFKLLLFSAGDGPLSTTPPLSLRRFIAISCLPIKILEHPSEKSAPTNNRA 120
           +T FF+ WLA FKLLLF+AGDGPLS  PPL LRRF+A+SCLPIKIL++  EKS P NNR+
Sbjct: 61  STSFFIAWLANFKLLLFAAGDGPLSAEPPLPLRRFLALSCLPIKILQN--EKSCPNNNRS 120

Query: 121 QTLHEKPKSLLNYSIRALIVVLMVKLYDYSEFLHPKLVLFIYSWHVYLLLEIILAITQFF 180
            T   KPKS LNYSI+A+IV LMVKLYDYSEF+HPK+VLFIYSWH+Y LLEIILAITQFF
Sbjct: 121 DTTL-KPKSFLNYSIKAVIVALMVKLYDYSEFIHPKVVLFIYSWHLYFLLEIILAITQFF 180

Query: 181 GRNLLGIELEPQFRDPYYSTSLQDFWGRRWNLMATNILQPAVYKPTVKMAAKVIGRMWAP 240
           GRN+LGIELEPQF+DPY+STSLQDFWGRRWNLMATNILQP VYKP+VK+AAKVIGR WAP
Sbjct: 181 GRNVLGIELEPQFQDPYFSTSLQDFWGRRWNLMATNILQPTVYKPSVKIAAKVIGRKWAP 240

Query: 241 LPGVMATFLVSAMMHELIFYYLGRMRPDWEITWFFVIHGIALTVEIMMKKMLPEKRRLPA 300
           LP VMATFLVSAMMHELIFYY+GRMRP+WE TWFFVIHG+ALTVEIM+KK+LPEK RLP 
Sbjct: 241 LPAVMATFLVSAMMHELIFYYMGRMRPNWECTWFFVIHGVALTVEIMVKKVLPEKWRLPP 300

Query: 301 KVSVPVTLVFIFSTAIWLFFPQFVRLKLDVRAFEEYAALANFVKKVAFSLVG 352
            VSVPVTL FIF TAIWLFFPQFVRLKLDVRAFEEYAAL  FVK VAFSLVG
Sbjct: 301 AVSVPVTLAFIFPTAIWLFFPQFVRLKLDVRAFEEYAALVTFVKNVAFSLVG 349

[Close]

BLAST of IVF0018138 vs. TAIR 10
Match: AT3G51970.1 (acyl-CoA sterol acyl transferase 1 )

HSP 1 Score: 295.0 bits (754), Expect = 7.7e-80
Identity = 160/344 (46.51%), Postives = 224/344 (65.12%), Query Frame = 0


Query: 5   IVNLTKVWLTVCISLSYCHATGKLLNPGVARFLAIAPIVLLFFLLPLQLTSIHFGGATCF 64
           + +  K W  V ISL Y     KL+  G+ R +   P+ L+FF++P  + S+H  G T F
Sbjct: 1   MASFIKAWGLVIISLCYTFFIAKLVPKGIKRLILFFPVFLIFFIVPFLIYSLHLLGITAF 60

Query: 65  FVTWLATFKLLLFSAGDGPLSTT-PPLSLRRFIAISCLPIKILEHPSEKSAPTNNRAQTL 124
           F+ WLA FKLLLF+ G GPLS+   PLSL  F+A+SCLPIKI   P     PT   +   
Sbjct: 61  FIAWLANFKLLLFALGRGPLSSNHKPLSLPIFLAVSCLPIKIQLSPK----PTKTHS--- 120

Query: 125 HE-KPKSLLNYSIRALIVVLMVKLYDYSEFLHPKLVLFIYSWHVYLLLEIILAITQFFGR 184
           HE   +  L Y+I+A+ VVL++K Y+YS  L  K+VL +Y+ H+Y  LEIILA T    R
Sbjct: 121 HEGSTEGPLIYTIKAVFVVLIIKAYEYSTKLPEKVVLTLYAIHIYFALEIILAATAAAVR 180

Query: 185 NLLGIELEPQFRDPYYSTSLQDFWGRRWNLMATNILQPAVYKPTVKMAAKVIGRMWAPLP 244
            +  +ELEPQF  PY +TSLQDFWGRRWNLM T IL+P VY+P++++ + V+G  ++ + 
Sbjct: 181 AMSDLELEPQFNKPYLATSLQDFWGRRWNLMVTGILRPTVYEPSLQLFS-VLGPNYSQIL 240

Query: 245 GVMATFLVSAMMHELIFYYLGRMRPDWEITWFFVIHGIALTVEIMMKKMLPEKRRLPAKV 304
               TF+VS +MHELIF+Y+GR+RPDW++ WFF+I+G   TVEI +KK +  + R P  +
Sbjct: 241 AAFGTFVVSGIMHELIFFYMGRLRPDWKMMWFFLINGFCTTVEIAIKKTINGRWRFPKAI 300

Query: 305 SVPVTLVFIFSTAIWLFFPQFVRLKLDVRAFEEYAALANFVKKV 347
           S  +TL F+  TA+WLF P+F R  +  +A +EYAA+  F  +V
Sbjct: 301 SQVLTLTFVMVTALWLFLPEFNRCNIVEKALDEYAAIGAFAVEV 336

[Close]

BLAST of IVF0018138 vs. TAIR 10
Match: AT5G55340.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 254.6 bits (649), Expect = 1.2e-67
Identity = 142/347 (40.92%), Postives = 208/347 (59.94%), Query Frame = 0


Query: 1   MEGEIVNLTKVWLTVCISLSYCHATGKLLNPGVARFLAIAPIVLLFFLLPLQLTSIHFGG 60
           M+ E+ NL KVW++  IS+SYC+     +  G  RFL+++P++ LF +LPL  +S+H   
Sbjct: 1   MDEELKNLIKVWVSAIISISYCYYIPPRIKSGAPRFLSVSPVLALFLVLPLFFSSLHLSL 60

Query: 61  ATCFFVTWLATFKLLLFSAGDGPLSTTPPLSLRRFIAISCLPIKILEHPSEKSAPTNNRA 120
            T FF+TWLA FKL+LFS   GPL    P +  RF   +C PIK+ ++P  +        
Sbjct: 61  ITAFFLTWLANFKLILFSFDKGPLIPI-PTNFPRFFCFTCFPIKVQQNPKSQ-------- 120

Query: 121 QTLHEKPKSLLNYSIRALIVVLMVKLYDYSEFLHPKLVLFIYSWHVYLLLEIILAITQFF 180
              +  PK  L ++I+  I  +++ LY Y + L P ++L +Y  H+YL +EIIL   +  
Sbjct: 121 ---NHLPK--LVFAIKLAIFAVLLHLYSYRQNLSPTILLGLYFVHLYLEIEIILTFVKVV 180

Query: 181 GRNLLGIELEPQFRDPYYSTSLQDFWGRRWNLMATNILQPAVYKPTVKMAAKVIGRMWAP 240
               LG +LEPQ   PY +TSLQDFWGRRWNLM   IL+PAVY P  +++ + +   WA 
Sbjct: 181 VFISLGCDLEPQSNKPYLATSLQDFWGRRWNLMVPAILRPAVYAPMRRVSERKMSSGWAL 240

Query: 241 LPGVMATFLVSAMMHELIFYYLGRMRPDWEITWFFVIHGIALTVEIMMKK--MLPEKRRL 300
            PG++A F+VS ++HEL+F+YL R  P  E+T FFV+HG+   VE+ +KK   + ++ RL
Sbjct: 241 FPGILAAFIVSGLVHELLFFYLIREMPTGEVTLFFVLHGVCTAVELAVKKKTTVAQRWRL 300

Query: 301 PAKVSVPVTLVFIFSTAIWLFFPQFVRLKLDVRAFEEYAALANFVKK 346
              VS  +T+ F+F T  WLF PQ  R  +  R   E   L  F+K+
Sbjct: 301 SPGVSRVLTVGFVFVTGGWLFTPQLKRSGVMERFTSEAVLLVEFIKR 333

[Close]

BLAST of IVF0018138 vs. TAIR 10
Match: AT5G55350.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 251.1 bits (640), Expect = 1.3e-66
Identity = 141/351 (40.17%), Postives = 208/351 (59.26%), Query Frame = 0


Query: 1   MEGEIVNLTKVWLTVCISLSYCHATGKLLNPGVARFLAIAPIVLLFFLLPLQLTSIHFGG 60
           ME E+++L KVW+   IS+SYC+ T   +  GV R L++ P+ +LF +LPL ++S+HF G
Sbjct: 1   MEEELMSLIKVWVYAIISISYCYYTSTRIKSGVFRLLSVLPVCVLFLVLPLFVSSVHFSG 60

Query: 61  ATCFFVTWLATFKLLLFSAGDGPLSTTPPLSLRRFIAISCLPIKILEHPSEKSAPTNNRA 120
           +T FF++WLA FKL+LFS   GPL   P  +L RF+  +C PIK+ ++P  +        
Sbjct: 61  STAFFLSWLANFKLILFSFDQGPLFPVPS-NLSRFVCFTCFPIKLQQNPKPQ-------- 120

Query: 121 QTLHEKPKSLLNYSIRALIVVLMVKLYDYSEFLHPKLVLFIYSWHVYLLLEIILAITQFF 180
              ++ PK    ++++     +++ +Y+Y + + P ++L +YS H+YL  EI+LA  +  
Sbjct: 121 ---NQMPK--WGFAVKLAFFGVLLHMYEYKQHMSPTVLLVLYSLHIYLEYEILLAPLKVL 180

Query: 181 GRNLLGIELEPQFRDPYYSTSLQDFWGRRWNLMATNILQPAVYKPTVKMAAKVIGRMWAP 240
               L  +LEP F +PY STSLQDFWGRRWNLM   IL+PAVY P  +MA + +    A 
Sbjct: 181 LSISLWCDLEPHFNEPYLSTSLQDFWGRRWNLMVPAILRPAVYLPVRQMAGRKMNSDQAL 240

Query: 241 LPGVMATFLVSAMMHELIFYYLGRMRPDWEITWFFVIHGIALTVEIMMKK-MLPEKRRLP 300
             GV A+FLVS ++HELIF+Y  R  P  E+T FFV+HG+    E   K+  L  + ++ 
Sbjct: 241 FLGVFASFLVSGVVHELIFFYFTRESPTGEVTLFFVLHGVCTAAECAAKRTRLVRRWKVS 300

Query: 301 AKVSVPVTLVFIFSTAIWLFFPQFVRLKLDVRAFEEYAALANFVKKVAFSL 351
             VS  +T+ F+  T  WLFFP   R  +  R  +E      FVK   F L
Sbjct: 301 QMVSRLLTVGFVVMTGGWLFFPHLARSGMIERLADEAFLFIGFVKHKFFYL 337

[Close]

BLAST of IVF0018138 vs. TAIR 10
Match: AT5G55370.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 248.8 bits (634), Expect = 6.4e-66
Identity = 145/349 (41.55%), Postives = 209/349 (59.89%), Query Frame = 0


Query: 1   MEGEIVNLTKVWLTVCISLSYCHATGKLLNPGVARFLAIAPIVLLFFLLPLQLTSIHFGG 60
           ME E+ NL KVW++  IS+SYC+     ++ GV R L++ PI ++F LLPL  +S+HF  
Sbjct: 1   MEEELRNLIKVWISALISISYCYYISSKISKGVLRLLSLLPIFIIFLLLPLFFSSVHFCV 60

Query: 61  ATCFFVTWLATFKLLLFSAGDGPLSTTPPLSLRRFIAISCLPIKILEHPSEKSAPTNNRA 120
            + FF TWLA FKL LF+    PLS  P  +L RF   +C PIKI ++PS      +NR 
Sbjct: 61  ISGFFFTWLANFKLFLFAFDQEPLSPLPS-NLTRFFCFACFPIKINKNPS------SNR- 120

Query: 121 QTLHEKPKSLLNYSIRALIVVLMVKLY--DYSEFLHPKLVLFIYSWHVYLLLEIILAITQ 180
             +H KP S    + + LI   ++ +Y  +Y   L     L +++ HVYL  E+IL    
Sbjct: 121 --IHNKPMSKWVLAFKLLIFSFLLHVYRNNYDSGLSRFAFLALFTIHVYLEAELILVFVG 180

Query: 181 FFGRNLLGIELEPQFRDPYYSTSLQDFWGRRWNLMATNILQPAVYKPTVKMAAKVIGRMW 240
                LLG E+EP F DPY +TSLQ+FW RRWNLM   +L+PAV+ P  +  A ++G   
Sbjct: 181 ALMSMLLGCEMEPVFNDPYLATSLQEFWSRRWNLMVPAVLRPAVHIPVQRFCAPLLGLHR 240

Query: 241 APLPGVMATFLVSAMMHELIFYYLGRMRPDWEITWFFVIHGIALTVEIMMKKM--LPEKR 300
           A   G++ATF+VS +MHELI++Y+ R  P WE+T FF++HG+   +EI MK+M  LP  R
Sbjct: 241 AFYAGMLATFIVSGLMHELIYFYVIRKSPTWEVTCFFLLHGVVTCLEIAMKRMRWLPTPR 300

Query: 301 RLPAKVSVPVTLVFIFSTAIWLFFPQFVRLKLDVRAFEEYAALANFVKK 346
           R  + +++    VF+  TA WLF+PQ +R  +  R   E   + + VK+
Sbjct: 301 RAVSGLAI---TVFLLVTAGWLFYPQMLRNDVHKRVISECLLVIDVVKR 336

[Close]

BLAST of IVF0018138 vs. TAIR 10
Match: AT5G55380.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 247.7 bits (631), Expect = 1.4e-65
Identity = 138/347 (39.77%), Postives = 204/347 (58.79%), Query Frame = 0


Query: 1   MEGEIVNLTKVWLTVCISLSYCHATGKLLNPGVARFLAIAPIVLLFFLLPLQLTSIHFGG 60
           ME +  NL +VW++  ISLSYC+     L+ GV R L+I P+ +LF +LPL L+ +HF  
Sbjct: 1   MEEKFRNLIEVWISALISLSYCYYISSKLSKGVLRLLSILPVCILFLVLPLFLSCVHFCA 60

Query: 61  ATCFFVTWLATFKLLLFSAGDGPLSTTPPLSLRRFIAISCLPIKILEHPSEKSAPTNNRA 120
            +  F++WLA FKLLLF+  +GPL   PP  L RFI  +CLPIKI + PS  + P     
Sbjct: 61  ISVLFLSWLANFKLLLFAFDEGPLFPLPP-KLSRFICFACLPIKIRQDPSPNAIP----- 120

Query: 121 QTLHEKPKSLLNYSIRALIVVLMVKLYDYSEFLHPKLVLFIYSWHVYLLLEIILAITQFF 180
             LH KP      +++ L++ +++ +Y+Y + L   +VL +Y  H+YL +E++L      
Sbjct: 121 -NLHPKPMPKWVLAVKILVLGVLLHVYEYRDGLPRFVVLALYCLHIYLEVELVLVFVGAV 180

Query: 181 GRNLLGIELEPQFRDPYYSTSLQDFWGRRWNLMATNILQPAVYKPTVKMAAKVIGRMWAP 240
              LLG  +EP F +PY +TSLQDFW RRWNLM + +L+  V+ P  +   +++    A 
Sbjct: 181 VSTLLGCNIEPVFNEPYLATSLQDFWSRRWNLMVSAVLRSTVHIPVQRFFKRILSPDGAM 240

Query: 241 LPGVMATFLVSAMMHELIFYYLGRMRPDWEITWFFVIHGIALTVEIMMKK---MLPEKRR 300
             GVMA+F VS +MHEL+++Y+ R  P WE+T FFV+HG A   EI +K+   + P  R 
Sbjct: 241 FAGVMASFFVSGLMHELLYFYMIRKPPTWEVTCFFVLHGAATATEIAVKRTQWLRPPHR- 300

Query: 301 LPAKVSVPVTLVFIFSTAIWLFFPQFVRLKLDVRAFEEYAALANFVK 345
               VS  V L F+  T +WLF  Q +R  +  +A  E   + +  K
Sbjct: 301 ---AVSGLVVLTFVSVTGVWLFLAQVLRNNVHEKAIGECLLVLDLAK 336

The following BLAST results are available for this feature:

BLAST of IVF0018138 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SV07	1.1e-78	46.51	Acyl-CoA--sterol O-acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=ASAT1 PE...	[more]
Q9FJ76	1.6e-66	40.92	Probable long-chain-alcohol O-fatty-acyltransferase 5 OS=Arabidopsis thaliana OX...	[more]
Q9XGY6	6.2e-66	38.50	Long-chain-alcohol O-fatty-acyltransferase OS=Simmondsia chinensis OX=3999 PE=1 ...	[more]
Q9FJ75	1.8e-65	40.17	Probable long-chain-alcohol O-fatty-acyltransferase 4 OS=Arabidopsis thaliana OX...	[more]
Q9FJ73	9.0e-65	41.55	Probable long-chain-alcohol O-fatty-acyltransferase 2 OS=Arabidopsis thaliana OX...	[more]

BLAST of IVF0018138 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L9S2	4.5e-184	93.47	MBOAT_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G684690 PE=...	[more]
A0A6J1J5C4	8.2e-162	79.77	acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC1114...	[more]
A0A6J1GBE5	1.4e-161	82.39	acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita moschata OX=3662 GN=LOC11...	[more]
A0A6J1KAA2	1.2e-160	81.53	acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC1114...	[more]
A0A6J1F6Y6	2.0e-160	78.63	acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita moschata OX=3662 GN=LOC11...	[more]

BLAST of IVF0018138 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004149740.1	6.55e-235	93.47	acyl-CoA--sterol O-acyltransferase 1 [Cucumis sativus]	[more]
KGN58563.1	1.79e-234	93.47	hypothetical protein Csa_002202 [Cucumis sativus]	[more]
XP_023527814.1	7.92e-206	79.77	acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita pepo subsp. pepo]	[more]
XP_022982638.1	1.12e-205	79.77	acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita maxima]	[more]
XP_022948970.1	2.99e-205	82.39	acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita moschata] >KAG6606895.1 Acy...	[more]

BLAST of IVF0018138 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G51970.1	7.7e-80	46.51	acyl-CoA sterol acyl transferase 1	[more]
AT5G55340.1	1.2e-67	40.92	MBOAT (membrane bound O-acyl transferase) family protein	[more]
AT5G55350.1	1.3e-66	40.17	MBOAT (membrane bound O-acyl transferase) family protein	[more]
AT5G55370.1	6.4e-66	41.55	MBOAT (membrane bound O-acyl transferase) family protein	[more]
AT5G55380.1	1.4e-65	39.77	MBOAT (membrane bound O-acyl transferase) family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR032805	Wax synthase domain	PFAM	PF13813	MBOAT_2	coord: 190..276 e-value: 4.6E-15 score: 55.6
IPR017088	Wax synthase, Magnoliopsida	PIRSF	PIRSF037006	Wax_synthase	coord: 1..352 e-value: 1.2E-117 score: 391.0
IPR044851	Wax synthase	PANTHER	PTHR31595	LONG-CHAIN-ALCOHOL O-FATTY-ACYLTRANSFERASE 3-RELATED	coord: 2..347
None	No IPR available	PANTHER	PTHR31595:SF46	ACYL-COA--STEROL O-ACYLTRANSFERASE 1	coord: 2..347

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
IVF0018138.1	IVF0018138.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006629	lipid metabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0008374	O-acyltransferase activity